Dr. Shobhit Singh - Academia.edu (original) (raw)
Papers by Dr. Shobhit Singh
SAE Technical Paper Series
International journal of scientific research, 2021
Aim:A prospective,randomized, double-blind,placebo-control study was carried out to compare intra... more Aim:A prospective,randomized, double-blind,placebo-control study was carried out to compare intravenous Esmolol and intravenous Nitroglycerin for attenuation of haemodynamic changes during laryngoscopy and intubation in patients posted for surgery under general anaesthesia. Material and Methods:The study was conducted on 90 patients of either sex divided in three groups (30 patients in each group),aged between 15-60 years. In Group C: 50 ml iv infusion of normal saline,Group E:100 micrograms/Kg/ml iv Esmolol infusion and Group N: 0.5 micrograms/Kg/ml iv Nitroglycerin infusion,(both drugs diluted to 50 ml) was given.Infusion in each group was started 5 minutes before induction and continued 5 minutes after intubation.Heart rate,systolic blood pressure,diastolic blood pressure,mean arterial pressure were recorded at 1,3,5 and 10 minutes post intubation and compared among groups. Results:Esmolol showed highly signicant(p<0.001) fall in heart rate at all time points post intubation. Decrease in SBP,DBP and MAP was more in Nitroglycerin group. Conclusion: We concluded that Esmolol was more effective in controlling the heamodynamic changes during laryngoscopy and intubation ,since no tachycardia was observed with Esmolol.
The utility of epigeic earthworm for successful degradation of organic wastes is well documented ... more The utility of epigeic earthworm for successful degradation of organic wastes is well documented for different industries such as paper and pulp. Compared to thermal composting, vermicomposting with earthworm often produces a product with a lower mass, lower processing time, and humus content; phytotoxicity is less likely; more N is released; fertilizer value is usually greater; and additional product (earthworms) which can have other uses is produced. Therefore, vermicomposting seems to be more appropriate and an efficient technology to convert industrial waste to a valuable community resources at low input basis. However, the composting efficiency and biology of only a few epigeic earthworm species has been studied, for example, Eisenia foetida. Compost is an excellent product; being homogenous and retaining most of the original nutrients and reduced levels of organic contaminants with respect to the starting material. It can be applied to soil to increase soil organic matter cont...
White Collar Crimes denote those anonymous, impersonal, indirect and difficult to detect variety ... more White Collar Crimes denote those anonymous, impersonal, indirect and difficult to detect variety of crimes which have been tagged as concentrated in the upper classes and many a times countermanding the requirement of mens rea. These differ from the conventional crimes in the genesis, pertinacity of intent and responsibility, pedagogics of criminology and penal philosophy as well as the pattern of law enforcement, trial procedure and sanction applied. Hence, this research paper pinpoints at length the characteristics, types, causes as well as the convolution involved in dealing with these crimes. The approach of Indian judiciary has also been traced down to uncover its leaning towards the repression of such crimes. Furthermore, the research paper diagnoses the fact that when there have been plurative white collar crimes in the nature of accounting scandals, corporate crimes, financial frauds, industrial espionage, instances of money laundering, mortgage fraud, securities fraud, terr...
Twenty-one tomato lines were evaluated under summer season in the field condition, the average da... more Twenty-one tomato lines were evaluated under summer season in the field condition, the average day and night temperature were 38.4 and 19.7°C, respectively. Several floral and fruit traits were adversely affected due to chronic high temperature stress which clearly indicated specific physiological processes of flower development were especiallysensitive tohigh temperaturestress. Flower drop are highly associated with styler exertion rate percentage. A significantincreasein flower drop(19.30%to74.36%), styler exertion (20.37% to 83.72%) and significantly decrease in pollen viability (14.78–74.55%) and fruit set truss−1 (15.45 to 75.37) were noted among the genotypes. On the basis of initial screening with respect to fruit set at high temperature stress, three lines i.e. CLN-2026, EC-538439 and EC-538148 emerged as high temperature tolerant genotypes. These lines, along with highly susceptible varieties i.e. Arka vikas, were critically observed for the stress tolerance traits. Most of the genotypes showed stigma exertion or tip burn under high temperature condition. Fruit number and fruit weight were the important yield components, which were severely affected under high temperature stress and ultimately yield was markedly reduced. Yield potential of the genotypes which was dependent on fruit set and fruit weight severely reduced under high temperature stress and yield varied from 386 g plant−1 (EC-538380) to 1832 g plant−1 (CLN-2026) in summer. On the basis of initial selection, it was concluded that the traits like fruit setting, pollen and yield attributes traits could be used in selection of high temperature tolerant genotypes for better fruit setting and tomato genotypes, i.e. CLN-2026 can be used as a source of high temperature tolerance genes for further breeding programs.
Dental Poster Journal, 2021
Most conventional dental materials have a neutral & passive existence in the oral environment. Th... more Most conventional dental materials have a neutral & passive existence in the oral environment. The majority of them were thought to be designed in a way that they could last for the more extended period in the oral cavity, permitting them to be utilized for a longer period 1. There is no single material in dentistry that is ideal and satisfies all the necessities of an ideal material 2. Science and innovation in the 21st century depends vigorously on the advancement of new materials that are relied upon to react to the changes in the environment and show their capacities as indicated by the ideal conditions 3. As the mission for an ideal remedial material proceeds, a more current age of materials was presented 4. Smart materials are a response to the necessity of environment amicable and responsive materials which modify their properties to perform explicit functions. These materials are named as 'Smart' as these materials uphold the rest of the tooth structure to the degree that more conservative cavity preparation can be completed 5. Depending on alterations in some external conditions, "smart" materials change their structure, composition, or their functions. Though, several stimuli can alter these materials including temperature, pH, moisture, electrical agents, chemical or biomedical stimuli, stress and magnetic fields, a key element of smart material incorporates a capacity to re-visitation of the original state after the stimulus has been removed 1. The various sorts of smart materials utilized in the field of dentistry are piezoelectric materials, shape memory alloys or polymers, pH delicate polymers, polymer gels, restorative materials such as smart ceramics (Cercon Zirconium), composites, impression materials, GIC, compomers, hybrid GIC, amorphous calcium phosphate releasing pit & fissure sealants, fluoridereleasing pit & fissure sealants etc. and others that have shown their own smart behavior such as smart suture, smart burs, etc 6. These are 'Biomimetic' in nature due to the properties of imitating normal tooth substance, for example, enamel or dentin 4. Their level of smartness is estimated as far as their "responsiveness" to natural upgrades and their "dexterity". These smart materials can undoubtedly detect the alterations in the oral cavity and react emphatically to these changes (are profoundly responsive and have an incredible ability to detect and react to any ecological change). Hence these materials are otherwise called "Responsive Materials" 7. It is the planning of biomaterials that animates the physical and mechanical properties of the lost tissues, consequently giving a chance to present and change treatment modalities for the infection. These advancements in material science have denoted the start of a period of bio-brilliant dentistry, a stage into what's to come 8. Today, it is one of the provoking assignments to produce new multifunctional materials that have insight at the material level. Material insight being arranged into three capacities: detecting changes in ecological conditions, handling the detected data lastly making judgment (impelling) by moving ceaselessly from or to the stimulus. This gathering of multipractical materials can have the ability to choose and execute explicit capacities smartly to react to changes in the neighbourhood condition 9. Biosmart dentistry is an interdisciplinary methodology and has the potential for changing ordinary dental practice by bringing up the intensity of modern science to take care of genuine clinical issues. These dynamic dental materials could potentially spearhead new groundbreaking dental therapies with a significantly enhanced clinical outcome of treatments 10. Furthermore, almost certainly the brilliant behavior of these biosmart materials holds a decent guarantee to the eventual fate of dentistry.
Dental Poster Journal, 2021
Regenerative dentistry is a gripping and developing field and has been recommended as a paradigm ... more Regenerative dentistry is a gripping and developing field and has been recommended as a paradigm shift in the management of oral wounds. The growing clinical interest to accelerate the healing kinetics using platelet derived products has led to the evolution of platelet derived concentrates. A new genera of platelet concentrates called "Platelet Rich Fibrin (PRF)" has been chronicled as an impetus in the therapeutic modality for dental diseases with its application in diverse disciplines in dentistry and more recently in Regenerative Endodontics and Endodontic Emergencies. Choukroun et al. first described PRF; has upped the ante in wound healing by providing 100% biocompatibility and acting as a natural scaffold enriched with growth factors for restoration and reconstitution of the lost architecture and function of tissues be it periodontal, periapical or be it bone regeneration. First application of PRF was done to improve bone healing in cases of implants 1. PRF is prepared by centrifuging a blood sample at 3000rpm for 10 min in a glass or glass coated plastic test tube. The resultant product consists of cellular plasma, PRF and RBCs 2. The middle layer of PRF clot is separated from the underlying RBCs and transferred on a sterile dish. The derived PRF is a concentrate of white blood cells, platelets, and fibrin 3. The fibrin matrix is composed of platelet cytokines, growth factors and cells which are released after a certain time and serve as a resorbable membrane. The mitogenic response in the periosteum for tissue repair is stimulated by the growth factors during normal wound healing process 4. These fibrin sealant properties and growth factors in platelets combined together provide an ideal base for healing of wounds and regeneration of tissues 2. It helps in accelerating the healing by prolonging the release of growth factors at the wound site, proliferation of fibroblasts and osteoblasts, promoting angiogenesis, inducing collagen synthesis, trapping the circulating stem cells and regulating the immunity, thus, making its application widespread 5. It is a biomodulator and has been shown to promote both hard and soft tissue healing without significant post-surgical inflammation. PRF has found its application in various fields of dentistry some of which are: management of extraction sockets, as a graft material for sinus elevations, for soft-tissue root coverage and for guided periodontal regeneration 6. Studies have proved it to be an excellent scaffolding material for management of diseased necrosed immature tooth for pulpal revival and tooth revitalization 7. Owing to its osteogenic potential, active research is also going on its application as a pulpotomy agent, and in surgical management of periapical lesion 8. These all above mentioned applications have become a reality owing to its advantage of being autologous in nature, easily accessible, presence of abundant growth factors, Increased healing kinetics of the grafted bone and economical as compared to the earlier used graft materials 9. Platelet concentrates thus have a great scope in the field of reconstructive and regenerative medicine and dentistry. It is newer, safer and simpler than the previous Platelet Rich Plasma concentrates hence they have found a wide and easy application in clinical dentistry practices of the contemporary era 6 .
African Journal of Microbiology Research, May 7, 2013
A total of 92 soybean nodulating rhizobia were isolated from different agro regions and screened ... more A total of 92 soybean nodulating rhizobia were isolated from different agro regions and screened for sensitivity against 8 rhizobiophages. Only 33 showed susceptibility with phage strains. Nineteen rhizobial isolates selected, including 9 isolates having high lytic behaviour and 10 resistant to all phages, were subjected to study on symbiotic effectiveness. Strains ALSR12, KNSR16, USDA123 and USDA94 showed highly significant interaction with host plant grown under axenic conditions. Strains USDA123 and ANSR12 had distinct lytic reaction with phage strain SR1 and SR2, respectively. Plaques formed by SR1 on USDA123 were quite clear with sharp margin, whereas central halo was surrounded by turbid concentric by SR2 on strain ALSR12. In view of generation time varying from 9.2 h (USDA94) to 12.6 h (ALSR12), they were marked as slow grower. Of the different carbon sources used, arabinose served as a potent carbon source for supporting highest growth of these strains. As compared to the other strain, ALSR12 was able to grow at 45°C and capable to tolerate a higher range of acidity and alkalinity. Strains ALSR12 and USDA123 showed highest resistance level with the antibiotics streptomycin and gentamicin, respectively. Ex-planta N 2-ase activity was determined only in USDA94 and ALSR12. This study provides means for conducting a systematic and scientific investigation towards obtaining a reliable understanding of the bradyrhizobial diversity in terms of their physiological behaviour.
Materials Chemistry and Physics, 2005
... nitrate) by a syringe. The color of the solutions changed to either yellow or goldenyellow du... more ... nitrate) by a syringe. The color of the solutions changed to either yellow or goldenyellow due to reduction of Ag + to Ag 0 . Stirring was continued under inert atmosphere at room temperature for another 15 min. The aliquots were ...
FEMS Microbiology Letters, 1994
Studies were carried out to examine the role of ammonium transport activity in the control of cae... more Studies were carried out to examine the role of ammonium transport activity in the control of caesium uptake and toxicity in Nostoc muscorum. The results showed a definite specific role of the ammonium-repressible/derepressible ammonium transport system of the cyanobacterium in caesium uptake, accumulation and toxicity. Furthermore, the results showed that N. muscorum can acquire resistance against diazotrophically-associated caesium toxicity when supplied with ammonium as a nitrogen source. In addition, alternatively, a mutant strain was Cs-resistant in the absence of any effect on NH~-transport, suggesting that Cs + resistance may be determined at more than one cellular site.
Contemporary Clinical Dentistry
Background: The orthodontic diagnosis and treatment planning are the fundamental goals during the... more Background: The orthodontic diagnosis and treatment planning are the fundamental goals during the mixed dentition period. Numerous methods have been proposed till date such as Nance method, Moyer's method, Staley–Kerber and Tanaka–Johnston's method, and Bernabé and Flores–Mir method. Aim: The aim of the study is to determine the mesiodistal widths of the lower permanent canines and premolars from Tanaka–Johnston, Moyers, and Bernabé E and Flores–Mir C mixed dentition analysis and to determine the correlation coefficients and the new prediction equation for Sri Ganganagar population. Setting and Design: A total of 3572 children were clinically examined from the contemporary population of Sri Ganganagar city and Outpatient Department of Pediatric and Preventive Dentistry in Sri Ganganagar, Rajasthan. A total of 150 individuals were randomly selected for the study based on the inclusion and exclusion criteria and were designated as “study samples. Materials and Methods: A total of 150 children aged 11–16 years of Sri Ganganagar city were randomly selected. The mesiodistal width of permanent incisors, canines, premolars, and molars was measured with the help of digital vernier caliper with an accuracy of ±0.01 mm. The measurements of canines and premolars were summed up and compared with those derived from Tanaka and Johnston equations, Moyers probability tables, and Bernabé and Flores-Mir equations. Statistical Analysis: The data obtained were subjected to statistical analysis using IBM SPSS Statistics Windows version “20.0” using Student's t-test, Pearson correlation coefficient, and Kruskal–Wallis test. Results: All the three methods overestimated the actual sum of permanent canine and premolars in both the arches in our population. The correlation coefficients and the new regression equations were derived for both maxilla and mandible in males and females for Sri Ganganagar population. Conclusion: The predicted values obtained from all the three methods overestimated the actual values.
Gastroenterology Report
Background Severe alcohol-associated hepatitis (SAH) patients with infections have a high short-t... more Background Severe alcohol-associated hepatitis (SAH) patients with infections have a high short-term mortality rate. Gut microbiota dysbiosis plays an important role in the pathogenesis of SAH. Preliminary studies have demonstrated long-term benefits with healthy donor fecal microbiota transplantation (FMT). Data on FMT compared with pentoxifylline for SAH and relevant gut microbial changes are lacking in literature. Methods From January 2019 to February 2021, retrospective analysis of a single hospital’s records revealed 47 SAH patients undergoing FMT (100 mL/day via nasoduodenal tube for 7 days) and 25 matched patients receiving pentoxifylline (400 mg/8 h for 28 days). The primary end point was a 6-month survival rate. Secondary end points included incidence of ascites, hepatic encephalopathy, infections, acute kidney injury, and gut microbiota changes between post-therapy groups. Biomarker discovery and network analysis were also performed to identify significant taxa of gut micr...
SAE Technical Paper Series
SAE Technical Paper Series
Earth Surface Processes and Landforms, 2022
Crop research, 2013
Field experiment was conducted on Agricultural Research Farm, Agra (U. P.) on a zinc deficient In... more Field experiment was conducted on Agricultural Research Farm, Agra (U. P.) on a zinc deficient Inceptisol soil to assess the effect of different rates, frequency and distribution of Zn in different fractions. Crop of wheat responded to applied Zn up to 22.5 kg/ha application of Zn at highest dose i. e. 30 kg ZnSO4/ha had effects up to wheat crop. For applied Zn on yield of wheat, a field experiment was undertaken to evaluate the effect of graded levels of zinc sulphate (0, 7.5, 15.0, 22.5 and 30.0 kg/ha) on the yield, content and uptake of nutrients in wheat. Different fractions of soil Zn were in dynamic equilibrium with each other. Depletion of water soluble, exchangeable and complexed forms of Zn occurred with a build-up organic, occluded and residual fractions of Zn. Depleted levels of readily available Zn in soil could be replenished by the other pools of soil Zn.
SAE Technical Paper Series
International journal of scientific research, 2021
Aim:A prospective,randomized, double-blind,placebo-control study was carried out to compare intra... more Aim:A prospective,randomized, double-blind,placebo-control study was carried out to compare intravenous Esmolol and intravenous Nitroglycerin for attenuation of haemodynamic changes during laryngoscopy and intubation in patients posted for surgery under general anaesthesia. Material and Methods:The study was conducted on 90 patients of either sex divided in three groups (30 patients in each group),aged between 15-60 years. In Group C: 50 ml iv infusion of normal saline,Group E:100 micrograms/Kg/ml iv Esmolol infusion and Group N: 0.5 micrograms/Kg/ml iv Nitroglycerin infusion,(both drugs diluted to 50 ml) was given.Infusion in each group was started 5 minutes before induction and continued 5 minutes after intubation.Heart rate,systolic blood pressure,diastolic blood pressure,mean arterial pressure were recorded at 1,3,5 and 10 minutes post intubation and compared among groups. Results:Esmolol showed highly signicant(p<0.001) fall in heart rate at all time points post intubation. Decrease in SBP,DBP and MAP was more in Nitroglycerin group. Conclusion: We concluded that Esmolol was more effective in controlling the heamodynamic changes during laryngoscopy and intubation ,since no tachycardia was observed with Esmolol.
The utility of epigeic earthworm for successful degradation of organic wastes is well documented ... more The utility of epigeic earthworm for successful degradation of organic wastes is well documented for different industries such as paper and pulp. Compared to thermal composting, vermicomposting with earthworm often produces a product with a lower mass, lower processing time, and humus content; phytotoxicity is less likely; more N is released; fertilizer value is usually greater; and additional product (earthworms) which can have other uses is produced. Therefore, vermicomposting seems to be more appropriate and an efficient technology to convert industrial waste to a valuable community resources at low input basis. However, the composting efficiency and biology of only a few epigeic earthworm species has been studied, for example, Eisenia foetida. Compost is an excellent product; being homogenous and retaining most of the original nutrients and reduced levels of organic contaminants with respect to the starting material. It can be applied to soil to increase soil organic matter cont...
White Collar Crimes denote those anonymous, impersonal, indirect and difficult to detect variety ... more White Collar Crimes denote those anonymous, impersonal, indirect and difficult to detect variety of crimes which have been tagged as concentrated in the upper classes and many a times countermanding the requirement of mens rea. These differ from the conventional crimes in the genesis, pertinacity of intent and responsibility, pedagogics of criminology and penal philosophy as well as the pattern of law enforcement, trial procedure and sanction applied. Hence, this research paper pinpoints at length the characteristics, types, causes as well as the convolution involved in dealing with these crimes. The approach of Indian judiciary has also been traced down to uncover its leaning towards the repression of such crimes. Furthermore, the research paper diagnoses the fact that when there have been plurative white collar crimes in the nature of accounting scandals, corporate crimes, financial frauds, industrial espionage, instances of money laundering, mortgage fraud, securities fraud, terr...
Twenty-one tomato lines were evaluated under summer season in the field condition, the average da... more Twenty-one tomato lines were evaluated under summer season in the field condition, the average day and night temperature were 38.4 and 19.7°C, respectively. Several floral and fruit traits were adversely affected due to chronic high temperature stress which clearly indicated specific physiological processes of flower development were especiallysensitive tohigh temperaturestress. Flower drop are highly associated with styler exertion rate percentage. A significantincreasein flower drop(19.30%to74.36%), styler exertion (20.37% to 83.72%) and significantly decrease in pollen viability (14.78–74.55%) and fruit set truss−1 (15.45 to 75.37) were noted among the genotypes. On the basis of initial screening with respect to fruit set at high temperature stress, three lines i.e. CLN-2026, EC-538439 and EC-538148 emerged as high temperature tolerant genotypes. These lines, along with highly susceptible varieties i.e. Arka vikas, were critically observed for the stress tolerance traits. Most of the genotypes showed stigma exertion or tip burn under high temperature condition. Fruit number and fruit weight were the important yield components, which were severely affected under high temperature stress and ultimately yield was markedly reduced. Yield potential of the genotypes which was dependent on fruit set and fruit weight severely reduced under high temperature stress and yield varied from 386 g plant−1 (EC-538380) to 1832 g plant−1 (CLN-2026) in summer. On the basis of initial selection, it was concluded that the traits like fruit setting, pollen and yield attributes traits could be used in selection of high temperature tolerant genotypes for better fruit setting and tomato genotypes, i.e. CLN-2026 can be used as a source of high temperature tolerance genes for further breeding programs.
Dental Poster Journal, 2021
Most conventional dental materials have a neutral & passive existence in the oral environment. Th... more Most conventional dental materials have a neutral & passive existence in the oral environment. The majority of them were thought to be designed in a way that they could last for the more extended period in the oral cavity, permitting them to be utilized for a longer period 1. There is no single material in dentistry that is ideal and satisfies all the necessities of an ideal material 2. Science and innovation in the 21st century depends vigorously on the advancement of new materials that are relied upon to react to the changes in the environment and show their capacities as indicated by the ideal conditions 3. As the mission for an ideal remedial material proceeds, a more current age of materials was presented 4. Smart materials are a response to the necessity of environment amicable and responsive materials which modify their properties to perform explicit functions. These materials are named as 'Smart' as these materials uphold the rest of the tooth structure to the degree that more conservative cavity preparation can be completed 5. Depending on alterations in some external conditions, "smart" materials change their structure, composition, or their functions. Though, several stimuli can alter these materials including temperature, pH, moisture, electrical agents, chemical or biomedical stimuli, stress and magnetic fields, a key element of smart material incorporates a capacity to re-visitation of the original state after the stimulus has been removed 1. The various sorts of smart materials utilized in the field of dentistry are piezoelectric materials, shape memory alloys or polymers, pH delicate polymers, polymer gels, restorative materials such as smart ceramics (Cercon Zirconium), composites, impression materials, GIC, compomers, hybrid GIC, amorphous calcium phosphate releasing pit & fissure sealants, fluoridereleasing pit & fissure sealants etc. and others that have shown their own smart behavior such as smart suture, smart burs, etc 6. These are 'Biomimetic' in nature due to the properties of imitating normal tooth substance, for example, enamel or dentin 4. Their level of smartness is estimated as far as their "responsiveness" to natural upgrades and their "dexterity". These smart materials can undoubtedly detect the alterations in the oral cavity and react emphatically to these changes (are profoundly responsive and have an incredible ability to detect and react to any ecological change). Hence these materials are otherwise called "Responsive Materials" 7. It is the planning of biomaterials that animates the physical and mechanical properties of the lost tissues, consequently giving a chance to present and change treatment modalities for the infection. These advancements in material science have denoted the start of a period of bio-brilliant dentistry, a stage into what's to come 8. Today, it is one of the provoking assignments to produce new multifunctional materials that have insight at the material level. Material insight being arranged into three capacities: detecting changes in ecological conditions, handling the detected data lastly making judgment (impelling) by moving ceaselessly from or to the stimulus. This gathering of multipractical materials can have the ability to choose and execute explicit capacities smartly to react to changes in the neighbourhood condition 9. Biosmart dentistry is an interdisciplinary methodology and has the potential for changing ordinary dental practice by bringing up the intensity of modern science to take care of genuine clinical issues. These dynamic dental materials could potentially spearhead new groundbreaking dental therapies with a significantly enhanced clinical outcome of treatments 10. Furthermore, almost certainly the brilliant behavior of these biosmart materials holds a decent guarantee to the eventual fate of dentistry.
Dental Poster Journal, 2021
Regenerative dentistry is a gripping and developing field and has been recommended as a paradigm ... more Regenerative dentistry is a gripping and developing field and has been recommended as a paradigm shift in the management of oral wounds. The growing clinical interest to accelerate the healing kinetics using platelet derived products has led to the evolution of platelet derived concentrates. A new genera of platelet concentrates called "Platelet Rich Fibrin (PRF)" has been chronicled as an impetus in the therapeutic modality for dental diseases with its application in diverse disciplines in dentistry and more recently in Regenerative Endodontics and Endodontic Emergencies. Choukroun et al. first described PRF; has upped the ante in wound healing by providing 100% biocompatibility and acting as a natural scaffold enriched with growth factors for restoration and reconstitution of the lost architecture and function of tissues be it periodontal, periapical or be it bone regeneration. First application of PRF was done to improve bone healing in cases of implants 1. PRF is prepared by centrifuging a blood sample at 3000rpm for 10 min in a glass or glass coated plastic test tube. The resultant product consists of cellular plasma, PRF and RBCs 2. The middle layer of PRF clot is separated from the underlying RBCs and transferred on a sterile dish. The derived PRF is a concentrate of white blood cells, platelets, and fibrin 3. The fibrin matrix is composed of platelet cytokines, growth factors and cells which are released after a certain time and serve as a resorbable membrane. The mitogenic response in the periosteum for tissue repair is stimulated by the growth factors during normal wound healing process 4. These fibrin sealant properties and growth factors in platelets combined together provide an ideal base for healing of wounds and regeneration of tissues 2. It helps in accelerating the healing by prolonging the release of growth factors at the wound site, proliferation of fibroblasts and osteoblasts, promoting angiogenesis, inducing collagen synthesis, trapping the circulating stem cells and regulating the immunity, thus, making its application widespread 5. It is a biomodulator and has been shown to promote both hard and soft tissue healing without significant post-surgical inflammation. PRF has found its application in various fields of dentistry some of which are: management of extraction sockets, as a graft material for sinus elevations, for soft-tissue root coverage and for guided periodontal regeneration 6. Studies have proved it to be an excellent scaffolding material for management of diseased necrosed immature tooth for pulpal revival and tooth revitalization 7. Owing to its osteogenic potential, active research is also going on its application as a pulpotomy agent, and in surgical management of periapical lesion 8. These all above mentioned applications have become a reality owing to its advantage of being autologous in nature, easily accessible, presence of abundant growth factors, Increased healing kinetics of the grafted bone and economical as compared to the earlier used graft materials 9. Platelet concentrates thus have a great scope in the field of reconstructive and regenerative medicine and dentistry. It is newer, safer and simpler than the previous Platelet Rich Plasma concentrates hence they have found a wide and easy application in clinical dentistry practices of the contemporary era 6 .
African Journal of Microbiology Research, May 7, 2013
A total of 92 soybean nodulating rhizobia were isolated from different agro regions and screened ... more A total of 92 soybean nodulating rhizobia were isolated from different agro regions and screened for sensitivity against 8 rhizobiophages. Only 33 showed susceptibility with phage strains. Nineteen rhizobial isolates selected, including 9 isolates having high lytic behaviour and 10 resistant to all phages, were subjected to study on symbiotic effectiveness. Strains ALSR12, KNSR16, USDA123 and USDA94 showed highly significant interaction with host plant grown under axenic conditions. Strains USDA123 and ANSR12 had distinct lytic reaction with phage strain SR1 and SR2, respectively. Plaques formed by SR1 on USDA123 were quite clear with sharp margin, whereas central halo was surrounded by turbid concentric by SR2 on strain ALSR12. In view of generation time varying from 9.2 h (USDA94) to 12.6 h (ALSR12), they were marked as slow grower. Of the different carbon sources used, arabinose served as a potent carbon source for supporting highest growth of these strains. As compared to the other strain, ALSR12 was able to grow at 45°C and capable to tolerate a higher range of acidity and alkalinity. Strains ALSR12 and USDA123 showed highest resistance level with the antibiotics streptomycin and gentamicin, respectively. Ex-planta N 2-ase activity was determined only in USDA94 and ALSR12. This study provides means for conducting a systematic and scientific investigation towards obtaining a reliable understanding of the bradyrhizobial diversity in terms of their physiological behaviour.
Materials Chemistry and Physics, 2005
... nitrate) by a syringe. The color of the solutions changed to either yellow or goldenyellow du... more ... nitrate) by a syringe. The color of the solutions changed to either yellow or goldenyellow due to reduction of Ag + to Ag 0 . Stirring was continued under inert atmosphere at room temperature for another 15 min. The aliquots were ...
FEMS Microbiology Letters, 1994
Studies were carried out to examine the role of ammonium transport activity in the control of cae... more Studies were carried out to examine the role of ammonium transport activity in the control of caesium uptake and toxicity in Nostoc muscorum. The results showed a definite specific role of the ammonium-repressible/derepressible ammonium transport system of the cyanobacterium in caesium uptake, accumulation and toxicity. Furthermore, the results showed that N. muscorum can acquire resistance against diazotrophically-associated caesium toxicity when supplied with ammonium as a nitrogen source. In addition, alternatively, a mutant strain was Cs-resistant in the absence of any effect on NH~-transport, suggesting that Cs + resistance may be determined at more than one cellular site.
Contemporary Clinical Dentistry
Background: The orthodontic diagnosis and treatment planning are the fundamental goals during the... more Background: The orthodontic diagnosis and treatment planning are the fundamental goals during the mixed dentition period. Numerous methods have been proposed till date such as Nance method, Moyer's method, Staley–Kerber and Tanaka–Johnston's method, and Bernabé and Flores–Mir method. Aim: The aim of the study is to determine the mesiodistal widths of the lower permanent canines and premolars from Tanaka–Johnston, Moyers, and Bernabé E and Flores–Mir C mixed dentition analysis and to determine the correlation coefficients and the new prediction equation for Sri Ganganagar population. Setting and Design: A total of 3572 children were clinically examined from the contemporary population of Sri Ganganagar city and Outpatient Department of Pediatric and Preventive Dentistry in Sri Ganganagar, Rajasthan. A total of 150 individuals were randomly selected for the study based on the inclusion and exclusion criteria and were designated as “study samples. Materials and Methods: A total of 150 children aged 11–16 years of Sri Ganganagar city were randomly selected. The mesiodistal width of permanent incisors, canines, premolars, and molars was measured with the help of digital vernier caliper with an accuracy of ±0.01 mm. The measurements of canines and premolars were summed up and compared with those derived from Tanaka and Johnston equations, Moyers probability tables, and Bernabé and Flores-Mir equations. Statistical Analysis: The data obtained were subjected to statistical analysis using IBM SPSS Statistics Windows version “20.0” using Student's t-test, Pearson correlation coefficient, and Kruskal–Wallis test. Results: All the three methods overestimated the actual sum of permanent canine and premolars in both the arches in our population. The correlation coefficients and the new regression equations were derived for both maxilla and mandible in males and females for Sri Ganganagar population. Conclusion: The predicted values obtained from all the three methods overestimated the actual values.
Gastroenterology Report
Background Severe alcohol-associated hepatitis (SAH) patients with infections have a high short-t... more Background Severe alcohol-associated hepatitis (SAH) patients with infections have a high short-term mortality rate. Gut microbiota dysbiosis plays an important role in the pathogenesis of SAH. Preliminary studies have demonstrated long-term benefits with healthy donor fecal microbiota transplantation (FMT). Data on FMT compared with pentoxifylline for SAH and relevant gut microbial changes are lacking in literature. Methods From January 2019 to February 2021, retrospective analysis of a single hospital’s records revealed 47 SAH patients undergoing FMT (100 mL/day via nasoduodenal tube for 7 days) and 25 matched patients receiving pentoxifylline (400 mg/8 h for 28 days). The primary end point was a 6-month survival rate. Secondary end points included incidence of ascites, hepatic encephalopathy, infections, acute kidney injury, and gut microbiota changes between post-therapy groups. Biomarker discovery and network analysis were also performed to identify significant taxa of gut micr...
SAE Technical Paper Series
SAE Technical Paper Series
Earth Surface Processes and Landforms, 2022
Crop research, 2013
Field experiment was conducted on Agricultural Research Farm, Agra (U. P.) on a zinc deficient In... more Field experiment was conducted on Agricultural Research Farm, Agra (U. P.) on a zinc deficient Inceptisol soil to assess the effect of different rates, frequency and distribution of Zn in different fractions. Crop of wheat responded to applied Zn up to 22.5 kg/ha application of Zn at highest dose i. e. 30 kg ZnSO4/ha had effects up to wheat crop. For applied Zn on yield of wheat, a field experiment was undertaken to evaluate the effect of graded levels of zinc sulphate (0, 7.5, 15.0, 22.5 and 30.0 kg/ha) on the yield, content and uptake of nutrients in wheat. Different fractions of soil Zn were in dynamic equilibrium with each other. Depletion of water soluble, exchangeable and complexed forms of Zn occurred with a build-up organic, occluded and residual fractions of Zn. Depleted levels of readily available Zn in soil could be replenished by the other pools of soil Zn.