Dr.Surapon Tangvarasittichai - Academia.edu (original) (raw)

Papers by Dr.Surapon Tangvarasittichai

Journal of the Medical Technologist Association of Thailand, Apr 1, 2015

Chronic kidney disease (CKD) is one of the major complications and the major causes of morbidity ... more Chronic kidney disease (CKD) is one of the major complications and the major causes of morbidity and mortality in type 2 diabetes (T2DM) patients. Tight control of glucose and lipid levels in blood can reduce or delay these diabetic complications. T2DM has been shown to have increased oxidative stress. Two hundred and ninety two T2DM patients participated in the present study. T2DM with eGFR < 60 mL/min/1.73 m 2 were found to have significantly higher levels of oxidative stress, uric acid and triglyceride in the blood, but lower levels of high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol(LDL-C) than those inT2DM with eGFR ≥ 60 mL/min/1.73 m 2 , but no significant differences were detected in total cholesterol. Oxidative stress was negatively correlated with eGFR and correlated with N-acetyl-β-D-glucosaminidase (NAG) (p<0.05). Oxidative stress may be the major component in pathogenesis of kidney complications and its progression to end stage renal disease. The results may suggest the combination use of drugs or dietary supplementation of antioxidants to protect or delay these diabetic complications.

Siriraj Medical Journal - สารศิริราช, 2009

IMPORTANCE Formulating exercise recommendations for patients with hypertrophic cardiomyopathy is ... more IMPORTANCE Formulating exercise recommendations for patients with hypertrophic cardiomyopathy is challenging because of concern about triggering ventricular arrhythmias and because a clinical benefit has not been previously established in this population. OBJECTIVE To determine whether moderate-intensity exercise training improves exercise capacity in adults with hypertrophic cardiomyopathy. DESIGN, SETTING, AND PARTICIPANTS A randomized clinical trial involving 136 patients with hypertrophic cardiomyopathy was conducted between April 2010 and October 2015 at 2 academic medical centers in the United States (University of Michigan Health System and Stanford University Medical Center). Date of last follow-up was November 2016. INTERVENTIONS Participants were randomly assigned to 16 weeks of moderate-intensity exercise training (n = 67) or usual activity (n = 69). MAIN OUTCOMES AND MEASURES The primary outcome measure was change in peak oxygen consumption from baseline to 16 weeks. RESULTS Among the 136 randomized participants (mean age, 50.4 [SD, 13.3] years; 42% women), 113 (83%) completed the study. At 16 weeks, the change in mean peak oxygen consumption was +1.35 (95% CI, 0.50 to 2.21) mL/kg/min among participants in the exercise training group and +0.08 (95% CI, −0.62 to 0.79) mL/kg/min among participants in the usual-activity group (between-group difference, 1.27 [95% CI, 0.17 to 2.37]; P = .02). There were no occurrences of sustained ventricular arrhythmia, sudden cardiac arrest, appropriate defibrillator shock, or death in either group. CONCLUSIONS AND RELEVANCE In this preliminary study involving patients with hypertrophic cardiomyopathy, moderate-intensity exercise compared with usual activity resulted in a statistically significant but small increase in exercise capacity at 16 weeks. Further research is needed to understand the clinical importance of this finding in patients with hypertrophic cardiomyopathy, as well as the long-term safety of exercise at moderate and higher levels of intensity.

วารสารสาธารณสุขมหาวิทยาลัยบูรพา, Dec 5, 2011

Cancer can produce many different symptoms, some subtle and some not at all subtle. Some symptoms... more Cancer can produce many different symptoms, some subtle and some not at all subtle. Some symptoms develop early in the course of cancer and are therefore important warning signs that should be evaluated by a doctor. Measurement of DNA concentration in the plasma of cancer patients and healthy subjects aimed to screening test for people in risk of cancer. There were 200 subjects, 100 patients with cancers and 100 healthy subjects. Nucleospin ® XS was used to extract DNA in plasma of both groups, and then Qubit fluorometer was used to measure DNA concentration. Our results show that there were significant associations between plasma DNA concentration of cancer patients. The concentration of plasma DNA in cancer patients; 31.0 (20.0-72.0) ng/ml were higher than plasma DNA concentration of healthy subjects; 75.0 (25.0-607.0) ng/ml. This study may be useful for further investigations in risk of cancer.

The Public Health Journal of Burapha University - วารสารสาธารณสุขมหาวิทยาลัยบูรพา, Jun 8, 2012

ABSTRACT Objective: Lipid hydroperoxide (LOOH) and malondialdehyde (MDA), products of lipid perox... more ABSTRACT Objective: Lipid hydroperoxide (LOOH) and malondialdehyde (MDA), products of lipid peroxidation, are occurring cellular processes and involved in cell adhesion and proliferation, inflammatory responses, aging, and death. Lipid peroxidation plays an important role in the premature development of atherosclerosis. We aim to determine the LOOH, MDA, and total antioxidant capacity (TAC) as the markers of oxidative stress in type 2 diabetes (T2D) patients. Materials and Methods: The study included 206 participants stratified as 123 T2D (with 37 males and 86 females) and 83 healthy controls (with 18 males and 65 females) randomly selected from Phitsanulok residents. MDA, LOOH, TAC levels and others biochemical markers were measured from the blood samples of these participants. Results: LOOH and MDA levels were significantly higher (P<0.05), and the TAC was lower in T2D patients. There were associations between elevated LOOH (OR=12.86; 95% CI 5.23-31.63), MDA (OR=8.54; 95% CI 3.15-23.18), hypertriglyceridemia (OR=3.96; 95% CI 1.49-10.48), abdominal obesity (OR=4.05; 95% CI 1.58-10.35) and T2D adjust-ing for age and gender. Increased LOOH and MDA levels, and decreased TAC levels are indi-cated oxidative stress occurred in T2D patients. Conclusion: Our results revealed that T2D patients had elevated oxidative stress enzymes that may cause oxidative damage. Therefore, LOOH, MDA and TAC assays are the good mark-ers for predicting oxidative stress in T2D patients.

Journal of the Medical Technologist Association of Thailand, Sep 1, 2013

Background-Apolipoprotein B (apoB) plasma levels reflect the concentration of proatherogenic lipo... more Background-Apolipoprotein B (apoB) plasma levels reflect the concentration of proatherogenic lipoproteins very low-density lipoprotein and low-density lipoprotein (LDL), whereas non-high-density lipoprotein cholesterol (non-HDL-C) levels reflect the concentration of cholesterol transported by these particles. Methods and Results-The aim of our study was to compare apoB, non-HDL-C, LDL cholesterol (LDL-C), and other lipid markers as predictors of coronary heart disease (CHD) in a nested case-control study among 18 225 participants in the Health Professionals Follow-up Study. Among men who were free of diagnosed cardiovascular disease at the time of blood collection, 266 had nonfatal myocardial infarction or fatal CHD during 6 years of follow-up. Through the use of risk set sampling, control subjects were selected at a 2:1 ratio and matched with regard to age, date of blood collection, and smoking status. After adjustment for matching factors, the relative risk of CHD in the highest quintile compared with the lowest quintile was 2.76 (95% confidence interval [CI], 1.66 to 4.58) for non-HDL-C, 3.01 (95% CI, 1.81 to 5.00) for apoB, 1.81 (95% CI, 1.12 to 2.93) for LDL-C, 0.31 (95% CI, 0.18 to 0.52) for HDL-C, 2.41 (95% CI, 1.43 to 4.07) for triglycerides (all P trend Ͻ0.001), and 1.42 (95% CI, 0.86 to 2.32, P trend ϭ0.19) for lipoprotein(a). When non-HDL-C and LDL-C were mutually adjusted, only non-HDL-C was predictive of CHD. When non-HDL-C and apoB were mutually adjusted, only apoB was predictive; the relative risk was 4.18 (95% CI, 1.30 to 13.49; P trend ϭ0.02) for apoB compared with 0.70 (95% CI, 0.21 to 2.27; P trend ϭ0.72) for non-HDL-C. Triglycerides added significant information to non-HDL-C but not to apoB for CHD risk prediction. Conclusions-Although non-HDL-C and apoB were both strong predictors of CHD in this male cohort, more so than LDL-C, the findings support the concept that the plasma concentration of atherogenic lipoprotein particles measured by apoB is more predictive in development of CHD than the cholesterol carried by these particles, measured by non-HDL-C. (Circulation. 2005;112:3375-3383.

PubMed, May 1, 2010

Background: The fasting serum total cholesterol (TC), triglycerides (TG), high density lipoprotei... more Background: The fasting serum total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) levels are used to calculate following lipid ratios: TC/ HDL-C, TG/HDL-C, and LDL-C/HDL-C. Cholesterol retention fraction (CRF), non-HDL-C, LDL-C, TG and waist circumference (WC) one considered as markers for the identification of individuals with an increased risk for cardiovascular diseases (CVD). These individuals frequently show insulin resistance as well. We analyzed the association of lipoprotein ratios with the homeostasis model assessment of insulin resistance (HOMA-IR). Methods: Type 2 diabetes mellitus (T2D) patients (92) and 40 age match healthy controls were randomized from the Tapho Primary Health Care Unit and the area in the same district. The HOMA-IR was used to calculate for insulin resistance. The areas under the curves (AUC) of the receiver operating characteristic curves (ROC) were used to compare the power of these serum lipoprotein ratios markers. Results: All lipoprotein ratios, lipid profile, blood pressure, and WC were significantly higher in T2D patients as compared to healthy controls (P<0.05). TC/HDL-C ratio, TG/HDL-C ratio, non-HDL-C, WC, TG, and TC were significantly correlated with HOMA-IR (P<0.05) as obtained by Spearman correlation analysis. The largest AUC of the ROC curve was obtained with the TC/HDL-C ratio as one parameter. Conclusion: TC/HDL-C ratio, TG/HDL-C, ratio, Non-HDL-C, WC, TG, and TC can be used as the markers of insulin resistance and CVD risk in T2D patients.

Journal of the Medical Technologist Association of Thailand, Apr 1, 2010

In-house hematological control products for automated blood cell analyzer were prepared from citr... more In-house hematological control products for automated blood cell analyzer were prepared from citrate-phosphate-dextrose (CPD) fresh whole blood from healthy donors that had been already screened infectious marker. These whole bloods were removed plasma then added with additive solution. After fixed with the mixture of formaldehyde and glutaraldehyde, they were divided into three different values; normal, high and low value. The control products were measured 14 hematological parameters by using Sysmex XT 1800i automatic blood cell counter for 6 weeks for evaluated accuracy, precision and stability of control products by using percentage of coefficient variation (CV). The results of preparing 3 level value of hematologic control materials of WBC count, RBC count, Hb, Hct, MCV, MCH, MCHC, platelet count, RDW, % neutrophil, % lymphocyte, % monocyte, % eosinophil and % basophil by using fresh whole blood donors illustrated that the percentages CV of high value control material of hematologic parameters were 8.82, 1.06, 1.22, 4.23, 4.8, 0.76, 4.76, 6.28, 4.89, 5.07, 10.11, 27.1 and 13.91, respectively. The percentages CV of normal value control material were 4.73, 0.85, 1.29, 4.05, 3.53, 0.84, 3.82, 9.84, 4.37, 5.20, 8.23, 10.3 and 6.33, respectively. Including the percentages CV of low value control material were 3.0, 1.47, 3.58, 4.33, 3.86, 2.43, 4.62, 7.75, 2.88, 3.11, 5.69, 9.7 and 11.37, respectively. These results indicated that the percentages CV was less than the accepted value of Sysmex XT 1800i that WBC count and platelet count were less than 10, RBC count, Hb, Hct, MCV, MCH, MCHC and RDW were less than 5, while % neutrophil, % lymphocyte, % eosinophil were less than 15 and % monocyte was less than 40. This study showed that in-house hematological control products was suitable for internal quality control material at least for 5 weeks without any significant change from the original ( P > 0.05), except WBC count, Hct, % monocyte in the high value control, Hct, MCV, MCHC, % monocyte in normal value control and Hct, MCV, MCHC, RDW, % eosinophil in low value control.

Current Pharmaceutical Design, Mar 15, 2019

The journal of automatic chemistry, 1995

The performance of Boehringer Mannheim's BM/Hitachi 911 was evaluated for three months. The mean ... more The performance of Boehringer Mannheim's BM/Hitachi 911 was evaluated for three months. The mean coeffcient of variation (CV) of the within-run and between-run imprecision of the 16 analytes were less than 1"161o (range 0"47-2"381o) and 1"35% (range 0"62-2"93,,4o), respectively. A linearity study for the various assays covered clinically important levels. No relevant drift was observed during an eight-hour assay nor was any sample-related carry-over detected. In all cases, the regression analyses (slopes) of the results obtained from BM/Hitachi 911 and 717 were between the extreme values of 0"94 and 1"05. During the three months of operation, no major problem was encountered. The BM/Hitachi 911 was found to be easily operated, to require minimal attention and simple daily maintenance during operation.

Journal of the Medical Technologist Association of Thailand, Aug 1, 2015

Chronic disease is the major health problem in elderly accompanied with physical inactivity and b... more Chronic disease is the major health problem in elderly accompanied with physical inactivity and body pain especially in lack of exercise. The present study was aimed to determine the changes of biochemical markers following stick exercise in elderly. Significant reduction in blood urea nitrogen (BUN), uric acid (UA), triglycerides (TG), TG/HDL-C ratio, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and elevation of high density lipoprotein cholesterol (HDL-C) were observed after finishing the stick exercise. These evidences provided proof that stick exercise or increased physical activity can improve quality of life and chronic disease protection.

Journal of the Medical Technologist Association of Thailand, Apr 1, 2012

Elevation of oxidized low density lipoprotein-s2-Glycoprotein ( OxLDL-s2-GPI ) and lipoprotein(a)... more Elevation of oxidized low density lipoprotein-s2-Glycoprotein ( OxLDL-s2-GPI ) and lipoprotein(a) Lp(a) plays a central role in the development of atherosclerosis. The elevation of OxLDL-s2-GPIand Lp(a) levels in patients with stable coronary artery disease (CAD) patients demonstrated as the effective risk factors for future CAD. The study included 6 diabetes mellitus patients with stable CAD complication (CAD_DM) and 6 age matched healthy controls. OxLDL-s2-GPI, Lp(a), MDA levels and other metabolic variables were measured in these participants. Comparison between groups was assessed by using Mann-Whitney U test. Bivariate correlation of variables was assessed by Spearmann rank correlation. All data analysis was performed by using SPSS version 13.0. OxLDL-s2-GPI, Lp(a), MDA and other metabolic variables were signiicantly higher in CAD_DM patients than healthy controls (p < 0.05). n bivariate correlation, OxLDL-s2-GPIhad signiicant correlation with Lp(a), MDA and other CAD risk factors in CAD_DM patients (p < 0.05). We also used the mutivariate forward stepwise linear regression analysis of the signiicant variables showed that Lp(a) (s = 0.574, R square=0.330, p < 0.001 ), HDL-C (s=0.357 , R square=0.457 , p= 0.009) and MDA (s=0.352 , R square=0.565, p=0.008) were independent predictors of OxLDL-s2-GPIin CAD_DM patients. We also set the cut-off levels of these markers by using ROC curve. Elevated OxLDL-s2-GPI, Lp(a), and MDA levels in patients with CAD despite being clinically stable and under medical treatment. These may be useful as effective risk markers for future CAD.

The Bulletin of Chiang Mai Associated Medical Sciences, 1993

International Journal of Diabetes in Developing Countries, Mar 17, 2015

The present study tested the hypothesis that simple variables, such as waist circumference, hyper... more The present study tested the hypothesis that simple variables, such as waist circumference, hypertriglyceridemia (eTG), and hypertriglyceridemic waist (eTGWC) phenotype could be used as screening tools for the identification of those at high risk in hypertension (HT) and type 2 diabetes mellitus (T2DM). Based on data from our health survey, check of 4206 participants (997 men and 3209 women) were 420 (42.1 %) and 2394 (74.6 %) AO men and women, 370 (37.1 %) and 1340 (41.8 %) eTG men and women, and 236 (23.7 %) and 1131 (35.2 %) eTGWC men and women. We demonstrated that AO, eTG, and eTGWC phenotype associated with increased risk of HT and T2DM. In men, the largest area under curve (AUC) of the receiver operating characteristic (ROC) curves demonstrated that AO was the best marker for HT and eTG was the best marker for T2DM in men, while eTGWC was the best marker for both HT and T2DM in women. In conclusion, it is suggested AO may serve as the early phenotype-associated sequel to eTG reach to eTGWC. Waist circumference measurements and fasting triglycerides can serve as inexpensive screening tools for HT and T2DM to identify those at high risk of HT and T2DM.

The erythrocyte sedimentation rate (ESR) measurement is the most widely used in laboratory test t... more The erythrocyte sedimentation rate (ESR) measurement is the most widely used in laboratory test to assess an inflammation. We try to introduce our two rapid alternative methods for ESR measurement. Four hundred forty nine blood samples were performed the ESR measurements by using Westergren, Wintrobe with centrifugation (Win-C) and capillary tube (Cap-C) with centrifugation methods. Win-C and Cap-C methods were significantly correlated with Westergren method by using Spearman rank correlation. Both Win-C and Cap-C methods were demonstrated the good % of sensitivity, % of specificity, % of positive predictive value, % of negative predictive value and cut-off point by using the receiver operating characteristic (ROC) curves analysis. Both methods demonstrated acceptable agreement with the conventional ESR measurements and appear to be a faster, easier and safety.

Objectives: Obesity is an important risk factor for type 2 diabetes mellitus (T2D), hypertention,... more Objectives: Obesity is an important risk factor for type 2 diabetes mellitus (T2D), hypertention, dyslipidemia, cardiovascular disease and cancers at several sites. The first aim of this study was to assess the prevalence of abdominal obesity (AO), hypertension, and dyslipidemia as health problems. A second aim was to determine the association between AO, hypertension and dyslipidemia as a metabolic risk factor for T2D. Materials and Methods: Throughout 2005 a small laboratory on the Mobile Medical Unit (MMU) for screening the rural populace during health check-ups was used to report health problems of the rural Thai population. A total of 1312 participants were measured for waist circumference, blood pressure, fasting blood glucose, total cholesterol, and triglyceride levels. Odds ratios of hypertension, AO and dyslipidemia and the associated risk factors of T2D and hypertension were calculated by multivariate logistic regression analysis. Results: Those with diabetes were 8.2% (10...

Journal of the Medical Technologist Association of Thailand, 2015

Type 2 diabetes mellitus (T2DM) patients have increased oxidative stress, inflammation and reduce... more Type 2 diabetes mellitus (T2DM) patients have increased oxidative stress, inflammation and reduced antioxidant defenses mechanisms in the circulatory system. This study demonstrates the progression and correlation of oxidative stress and inflammation with chronic kidney disease (CKD), pre-hemodialysis and hemodialysis in T2DM patients. A total of 292 T2DM patients participated in the present study. These T2DM patients’ diagnoses ranged from stage 1 to 5 according to their estimated glomerular filtration rate (eGFR). Serum levels of high-sensitive C-reactive protein (hs-CRP) and malondialdehyde (MDA) were significantly increased, but total antioxidant capacity (TAC) in these T2DM patients was decreased. The eGFR was inversely correlated with MDA, hs-CRP, NAG, TG/HDL-C and positively correlated with HDLC and TAC. Hs-CRP and TG/HDL-C were positively correlated with MDA, and negatively correlated with TAC and HDLC in the present study. Multiple forward stepwise linear regression analyse...

Diabetes & Obesity International Journal, 2017

Dyslipidemia is a common occurrence in type 2 diabetes mellitus (T2DM) patients and plays the maj... more Dyslipidemia is a common occurrence in type 2 diabetes mellitus (T2DM) patients and plays the major role in accelerated risk of cardiovascular disease (CVD) and possible causes chronic kidney disease (CKD), the progression of which leads to end-stage renal disease. A total of 266 T2DM patients were categorized into 2 groups according to their TG/HDL-C ratio of 2.5 and <2.5. The comparison of the clinical characteristics in these 2 groups demonstrated that Glucose, HbA1c, total Cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) levels, TG/HDL-C ratio were significantly higher, while high density lipoprotein cholesterol (HDL-C) and estimated glomerular filtration rate (eGFR) were significantly lower in the group with TG/HDL-C ratio 2.5 (p<0.05). Multiple logistic regressions demonstrated elevated TG/HDL-C ratio associated with CKD and increased HbA1c. The ORs and 95 % CIs were 4.94 (2.45, 9.96), 2.29 (1.13, 4.65) after adjusting for their covariates. Elevated TG/HDL-C ratio was associated with CKD and HbA1c and may increase the rate of disease progression and predict decline in kidney function and structural damage in these T2DM patients.