Dragan Gvozdic - Academia.edu (original) (raw)
Papers by Dragan Gvozdic
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Glas. Srpska akademija nauka i umetnosti. Odeljenje medicinskih nauka, 1992
The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the se... more The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the serum levels of thyroid hormones (T3 and T4) has been studied in gilts at various stages of reproductive cycle, as well as on the their reproductive performances. The gilts were the crossbred of Swedish Landrace x Big Yorkshire x German Landrace, selected for reproduction. The serum concentrations of T3 and T4 in four month old gilts (at the beginning of experiment) were normal: 1.48 +/- 0.28 and 46.57 +/- 11.37, respectively. Approximately the same levels of serum thyroid hormones were found in all 3 experimental groups of the gilts at the first oestrus (about 6.5 months) when they were artificially inseminated, at the end of the first third of the pregnancy, at the end of lactation and on the day of first postlactational oestrus. Significantly lower concentrations of serum T4 were only at 1-3 days before parturition, 6-12 hours after parturition and during lactation in all groups of gilts. There were no significant differences in T3 and T4 concentrations as well as in reproductive performances between gilts on the different iodine diet. The authors concluded that 1 p.p.m. of dietary iodine, which is regularly applied in this pig farm, is sufficient to provide normal levels thyroid hormones and normal reproduction. There is not economical and physiological reasons to increase this level of iodine to 2 and 3 p.p.m., since the obtained results showed that these changes don't demonstrated neither positive or negative effects.
Acta Veterinaria-beograd, 2011
The aim of this stady was to examine the relationship between triiodothyronine (T 3), thyroxin (T... more The aim of this stady was to examine the relationship between triiodothyronine (T 3), thyroxin (T 4), insulin-like growth factor-I (IGF-I) and blood serum liver function biomarkers concentrations in Holstein and Red Holstein heifers during the peripartal period. In the first experimental group (I) were heifers of Holstein Friesian breed (n=10), and in the second experimental group (II) were Red Holstein heifers (n=10). Blood samples were taken from the vena coccygea three times during the short peripartal period (5 days before delivery, at delivery and 5 days after delivery), and blood serum samples were separated after spontaneous coagulation. Blood serum T 3 , T 4 and IGF-I concentrations were determinated using radioimmunoassay (RIA). Blood serum concentrations of glucose (G), total protein (TP), albumin, (Alb), urea, total bilirubin (tBil) and aspartate aminotrasferase (AST) were determined using commercial ELISA kits. The statistical significance between means was determined using Student t-test. Blood serum T 3 concentration in Group II was significantly lower after delivery, and T4 concentration was significantly higher before delivery (p<0.05), compared to Group I. Mean blood serum IGF-I concentrations between the two experimental groups were not significantly different at all time intervals. Relationships between the determined hormones showed a median positive correlation between T 3 , and T 4 (r=0.74) in Group I and highly positive correlation in Group II (r=0.81). Blood serum thyroid gland hormones and IGF-I concentrations in healthy heifers during peripartal period vary considerably, the lowest values being determined immediately after calving in both experimental groups. Blood serum G, TP, Alb and urea concentrations were significantly higher in Group I at all investigated time intervals. Blood serum tBil concentration was significantly higher after partus in Group I (11.02 ± 3.33 : 8.49 ± 3.35 mmol/L, Group I vs. II, respectively), and AST activity was not significantly different at all intervals.
PubMed, 1992
The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the se... more The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the serum levels of thyroid hormones (T3 and T4) has been studied in gilts at various stages of reproductive cycle, as well as on the their reproductive performances. The gilts were the crossbred of Swedish Landrace x Big Yorkshire x German Landrace, selected for reproduction. The serum concentrations of T3 and T4 in four month old gilts (at the beginning of experiment) were normal: 1.48 +/- 0.28 and 46.57 +/- 11.37, respectively. Approximately the same levels of serum thyroid hormones were found in all 3 experimental groups of the gilts at the first oestrus (about 6.5 months) when they were artificially inseminated, at the end of the first third of the pregnancy, at the end of lactation and on the day of first postlactational oestrus. Significantly lower concentrations of serum T4 were only at 1-3 days before parturition, 6-12 hours after parturition and during lactation in all groups of gilts. There were no significant differences in T3 and T4 concentrations as well as in reproductive performances between gilts on the different iodine diet. The authors concluded that 1 p.p.m. of dietary iodine, which is regularly applied in this pig farm, is sufficient to provide normal levels thyroid hormones and normal reproduction. There is not economical and physiological reasons to increase this level of iodine to 2 and 3 p.p.m., since the obtained results showed that these changes don't demonstrated neither positive or negative effects.
Acta Veterinaria-beograd, 2010
The aim of this study was to test a new experimental design of venous system arterialization on t... more The aim of this study was to test a new experimental design of venous system arterialization on the rabbit ear arterialized venous flap (AVF) model. Total number of 10 "Big Chinchila" rabbits were divided in two experimental groups. On both ears of the five rabbits (Group 1) we have performed our original method of venous system arterialization with microsurgical arterialization of the central artery and vein with the preservation of central and peripheral vascular perfusion; at both ears of five rabbits (Group 2) we have performed AVF according to Byan et al., (1995). Vital AVF surface and necrosis percentage were determined in both experimental groups at day 1 and day 14 and results were compared using Student t-test. The results of our experiment indicate that our new experimental design of the AVF on rabbit ear model has better hemodynamic conditions, improves AVF survival and gives significantly bigger vital flap surface at 14 days after venous system arterialization.
Acta Veterinaria-beograd, 2004
The blood plasma concentrations of selenium and plasma and whole blood GSH-Px activities were det... more The blood plasma concentrations of selenium and plasma and whole blood GSH-Px activities were determined in 30 sheep on three farms (A =Ka}, B =E~ka and C=^oka) in marginally deficient areas of Vojvodina. Selenium concentrations in feedstuffs from the territories surrounding the studied farms were lower than 0.10 ppm in more than 90% of the samples. Plasma selenium concentrations in sheep from farms A, B and C were 83.
Journal of Dairy Research
This research paper addresses the hypothesis that an oral supplementation with organically modifi... more This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 ± 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 ± 4 and 4 ± 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at f...
The aim of this work was to determine incidence of calving and postcalving reproduction disorders... more The aim of this work was to determine incidence of calving and postcalving reproduction disorders and their effect on the major parameters of cows reproductive efficiency at dairy farm in the R. Serbia. Total number of 1835 high-milking dairy cows (av. milk production >8500L/305d of lactation) records has been reviewed during one year period, at the large dairy farm in area of Vojvodina (R. Serbia). Normal calving and puerperal period without health problems was observed at 583 cows (27,6%), while different abnormalities during and after calving were recorded in 1252 cows (72,4%). The highest percentages of registered peripartal disorders were dystocia and endometritis (62% and 64%, respectively), affecting almost the same percentage of cows. Peripartal disorders had statistically significant influence (P lt 0.05) on the increase of number of services/conception (3,5 vs. 2,7, reproductive disorders vs. healthy cows, respectively), prolongation of the interval from first postparta...
Acta Veterinaria, 2017
Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormon... more Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual influence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium deficiency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 m...
Acta Veterinaria, 2015
The aim of this investigation was to determine the influence of oxidative stress upon general str... more The aim of this investigation was to determine the influence of oxidative stress upon general stress in dairy cows on parturition and whether the indicators of stress can be reduced by selenium (Se) supplementation.A total of 36 animals were divided into 3 groups 21 days prepartum and treated with a single-term intramuscular supplement of sodium selenite: Control group - 0 mg; group Se10 - 10 mg; group Se20 - 20 mg.Se supplementation significantly raised blood Se content and glutathione peroxidase (GPx) activity in groups Se10 and Se20, compared to Control, although there was no marked difference between supplemented groups. Plasma malondialdehyde (MDA) and cortisol concentrations were significantly reduced in supplemented groups Se10 and Se20, compared to Control. A negative correlation was detected between blood GPx activity and plasma MDA, while a positive correlation was determined between plasma MDA and cortisol concentrations.These results indicate that prepartum Se supplement...
Veterinary Immunology and Immunopathology, 2012
Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' br... more Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc␥ and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecules were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc␥ receptors.
Acta veterinaria, 2006
The involvement of adrenocortical and thyroid hormones in the pathogenesis of ketosis, as well as... more The involvement of adrenocortical and thyroid hormones in the pathogenesis of ketosis, as well as the ovarian consequences of this metabolic disorder, were studied in ³2 parity cows (n=199) in 3 large scale dairy herds. To compare the plasma/serum concentrations of certain hormones Šcortisol, thyroxin (T 4), triiodothyronine (T 3), insulin, insulin-like growth factor-1 (IGF-1)¹ and metabolites Šglucose (G), acetoacetic acid (ACAC), bOH-butyrate (BHB), non-esterified fatty acid (NEFA), trigliceride (TG), total cholesterol (TCh)¹, and the activity of aspartate aminotransferase (AST), blood samples were taken 1 to 3 days after calving and again 4 times 7 days apart. The ACTH-challenged cortisol responsiveness and the TRH-induced T 4 /T 3 increase were determined between days 1 to 3 and again between days 28 to 35. The resumption of ovarian cyclicity was followed up by individual progesterone (P4) profiles based on milk samples taken 3 times a week for about 80 to 85 days. BHB level of 1 mmol/L was estimated as a border line between hyper-(>1 mmol/L) and normoketonaemic (<1 mmol/L) conditions. Five different ketone patterns were distinguished: (1) non-ketotic (n=98; normoketonaemia in all samples), (2) early type ketosis (n=45; hyperketonemia was detected only in the first week after calving), (3) late type (lactational) ketosis (n=11; after a normoketonaemic period increasing hyperketonaemia was detected in the 5 th , or in the 4 th and 5 th weeks), (4) temporary ketosis (n=11; hyperketonaemia was detected for 1-2 weeks in the 2 nd and 3 rd or in the 3 rd and 4 th weeks); (5) long-lasting ketosis (n=34; hyperketonaemia has been detected since calving for 4 to 5 weeks or until dying / emergency slaughtering). Simultaneously with the hyperketonaemic stage increased NEFA, ACAC, depressed TCh, glucose and decreased insulin, IGF-1, T 4 and T 3 concentrations were detected in almost all the cases. Obvious metabolic and endocrine alterations were found, however, only in long-lasting ketosis. The TRH-stimulated T 4 and T 3 responses remained almost unaffected proving intact thyroid function in early and late type as well as in temporary ketosis. Depressed thyroid
Acta veterinaria, 2010
Modern intensive pig production demands an increasing number of insemination doses per ejaculate ... more Modern intensive pig production demands an increasing number of insemination doses per ejaculate of genetically superior boars. In order to achieve such a result the possibility of producing insemination doses in both reduced volume and spermatozoa count without decreasing the fertility of sows is studied. In this trial we studied the effect of insemination with reduced volumes of semen (50 mL) and varied spermatozoa count (4, 2 or 1x10 9). Insemination was performed by the classical (intracervical) or by the new (intrauterine) techniques and the basic fertility parameters (farrowing % and litter size) were measured. The farrowing value decreased with decreasing spermatozoa numbers after intracervical and intrauterine insemination. However, the farrowing value, regardless of spermatozoa numbers, was significantly higher after intrauterine insemination (83.3%, 76.7%, 66.7%) compared to the classical intracervical insemination (73.3%, 66.7% and 50%). Litters size did not vary significantly depending on the applied insemination procedure or spermatozoa number. These results indicate that application of the new intrauterine insemination procedure enables the use of doses with a smaller volume and spermatozoa number, at the same time obtaining a satisfactory farrowing and litter size. This opens the possibility of obtaining a significantly higher number of doses per ejaculate when compared to the classical intracervical insemination procedure. In such a way can be increased significantly the degree of reproductive exploitation of genetically superior boars.
Acta veterinaria, 2012
The aim of this work was to investigate the impact of two energy supplements based on propylene g... more The aim of this work was to investigate the impact of two energy supplements based on propylene glycol in dairy cows diet on ovarian and follicular morphology, conception, insemination index and length of service period. A total number of 60 Holstein Friesian dairy cows, parity between 2-8, with an average milk production of 7000 kg/305 days of lactation were divided into three experimental groups (20 dairy cows per group). The first group of dairy cows was supplemented daily with "Energy-plus" (O1 group; 200 mL propylene-glycol supplement) and the second group was supplemented with "Ketal" (O2 group; 160 mL propylene-glycol supplement), two weeks before partus until 30 days post partum. The third experimental group were non supplemented dairy cows (O3, control group). Ultrasound examination of the reproductive system using real time echo camera Falco VET 100 (ESAOTE PieMedical, Holland, B-shaped scan with linear-array endorectal 5-8 MHz probe) was conducted on every animal starting from day 40 postpartum. The diameters of the ovaries (left and right) and of the dominant follicle(s) were recorded. Ultrasound testing was repeated on day 50 and 60 postpartum only in cows which in the meantime were not inseminated. Reproduction efficiency parameters (conception rate, number of inseminations and length of service period) were recorded individually. The statistical significance of the differences between groups was tested using ANOVA with LSD test at the level of significance p<0.05, chi-square test and Kaplan-Meier survival analysis (the length of service period). There was no significant impact of the propylene glycol supplementation on the ovarian and follicular morphology at the first ultrasound examination. At the second ultrasound examination there was a significant difference between left ovarian dominant follicle diameter in the control and supplemented dairy cows (1.67±0.53 vs 1.12±0.29 and 1.11±0.35 cm, p<0.05, O3 vs O1 and O2, respectively). The cumulative percentage of conception after the first
Acta veterinaria, 2013
The objective of this study was to determine the levels of insulin, insulin like growth factor I ... more The objective of this study was to determine the levels of insulin, insulin like growth factor I (IGF-I) and thyroid hormones in relation to the body condition score (BCS) of periparturient dairy cows. The study was carried out on twenty Holstein-Friesian dairy cows with average milk production of 7000 L/305 days in the previous lactation, parity ranging from 2-4. All cows were BCS scored during the early dry period, 7±3 days before and after parturition. Based on the BCS at the early dry period, cows were divided in two groups: cows with high BCS (3.75-4.25, HBCS, n=10), and cows with moderate BCS (2.75-3.75, MBCS, n=10). Blood samples were taken at the time of BCS evaluation. Concentrations of insulin, IGF-I, triiodothyroinine (T 3) and thyroxine (T 4) were determined by radioimmunoassay (RIA, INEP-Zemun, Serbia). Statistical differences between mean values were determined using Student t-test (p<0.01). Mean BCS values at early dry period were significantly different compared to two other periods (p<0.01) within both groups, but BCS was similar in both groups at 7±3 days after calving (2.69±0.67:2.62± 0.27, p>0.05). IGF-I level in HBCS cows at days 7±3 before calving was significantly higher (16.28±3.07:11.76±2.28, p<0.01), with a reverse relationship after calving (3.77±1.64:8.46±2.37, p<0.01). Insulin level was significantly lower at 7±3 days before calving in HBCS cows (16.26±4.60:20.18±4.96mIU/L, p<0.05). Thyroid hormones levels were significantly lower in HBCS group et all examined periods.
Veterinarski glasnik, 2020
Introduction. In order to provide the most favorable conditions for bone healing following implan... more Introduction. In order to provide the most favorable conditions for bone healing following implant loading, new techniques such as the use of growth factors to directly or indirectly regulate cell and tissue growth are becoming increasingly popular. Simplicity in producing concentrated thrombocytes derived from platelet-rich plasma has led to increasing application of growth factors derived from thrombocytes, such as PDGF, TGF-?, IGF and VEGF, in order to increase the percentage of bone-to-implant contact that provides better implant stability and possibly earlier functional loading. The aim of the study was to determine the effects of platelet-rich plasma and guided tissue regeneration on bone regeneration in the case of bone defects in early dental implant insertion. Materials and Methods. This experimental study was conducted on 10 study dogs that received 40 BCT implants, i.e., 4 implants per dog. The study included early dental implant placement in the sites of the extracted pr...
Research in Veterinary Science, 2016
In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia hav... more In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; viability. The strongest effect of inhibition of cows&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
Animal science papers and reports
The aim of this study was to investigate the relationship between the loss in body condition scor... more The aim of this study was to investigate the relationship between the loss in body condition score (BCS) and loss (ABCS), energy balance (EB), hepatic lipidosis and blood serum concentration of non esterified fatty acids (NEFA), glucose, triacylglycerol (TAG) and total bilirubin (tBIL) in healthy dairy cows during transition period. Twenty healthy Holstein cows were included and categorized into groups based on BCS loss (Delta BCS) between dry period and early lactation (Delta BCS <0.75 and >= 0.75). Significant differences between groups (p<0.05) were observed for blood serum NEFA, glucose and tBIL. Cows with high Delta BCS (>= 0.75) between dry period and early lactation showed increased blood serum NEFA, TAG and tBIL concentrations and lower blood serum glucose concentration
Slovenian Veterinary Research
The objective was to determine the effects of Regumate ® alone or Regumate ® +eCG treatment on th... more The objective was to determine the effects of Regumate ® alone or Regumate ® +eCG treatment on the synchronization of estrus and fertility in delayed puberty (n=30+30) and normal cyclic gilts (n=30+30). Gilts were fed daily a complete diet containing 20 mg/gilt Regumate ® for 18 days. Twenty-four hours after the last feeding of Regumate ® , all gilts received an i.m. injection of 1,000 IU eCG. Gilts were inseminated artificially (AI) 12 and 24 h after first detection of standing estrus. More normal cyclic gilts (84.6%) than delayed puberty gilts (66.7%) expressed estrus by d 4.8 after Regumate ® alone treatment (p < 0.05). However, similar proportion of normal cyclic (90%) and delayed puberty gilts (93%) were in estrus by d 4.2 after Regumate ® + eCG treatment (p > 0.05). These findings indicate that about 70% of delayed puberty gilts had established cyclic ovarian activity (i.e. sexually mature, cyclic gilts), while about 30% were truly delayed puberty gilts (sexually immatur...
Journal of Veterinary Medicine, 2013
The incidence of retained placenta (RP) in cows increases in cases of parturition induced by pros... more The incidence of retained placenta (RP) in cows increases in cases of parturition induced by prostaglandin F2α. We analyzed the effects of different doses of supplemental selenium and vitamin E on the incidence of RP, blood selenium, plasma thyronines, and malondialdehyde concentration. Thirty-three clinically healthy, multiparous Holstein-Frisian cows were assigned to 3 groups and supplemented with a single intramuscular injection of sodium selenite (SS) and tocopherol acetate (TAc) between days 250 to 255 of gestation: control—unsupplemented; group A—10 mg SS + 400 mg TAc; group B—20 mg SS + 800 mg TAc. Parturition was induced using PGF2α not before day 275 of gestation. The RP incidence was reduced from 66.7% in the control to 38.2 and 30.8% in groups A and B, respectively. Blood selenium and glutathione peroxidase activity in treated groups were significantly higher compared to control, with no significant difference between groups A and B. Plasma malondialdehyde in group B was ...
Veterinarski glasnik, 2010
The work presents the results of investigations of the effect of moderate heat stress on the acid... more The work presents the results of investigations of the effect of moderate heat stress on the acidobasal status in high-yield dairy cows in early lactation. Improving performance in high-yield dairy cows increases their inclination toward metabolic disorders. The most likely to be affected is the acid-base balance, in particular when cows are exposed to heat stress. Investigations so far have shown that the taking of urine samples and their analysis is the best and fastest way to diagnose disorders in the acid-base balance. Investigations were carried out on 7 clinically healthy cows of the Holstein- Friesian breed in the phase of early lactation, 30 to 40 days (on days 30, 33 and 40 ) following calving during the summer period, during the month of July, when there were significant variations in daily and nightly temperatures. The cows were in the second and fourth lactation, the annual milk yield was 8000 L milk per cow. The average daily milk production in the early phase of lactat...
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Glas. Srpska akademija nauka i umetnosti. Odeljenje medicinskih nauka, 1992
The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the se... more The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the serum levels of thyroid hormones (T3 and T4) has been studied in gilts at various stages of reproductive cycle, as well as on the their reproductive performances. The gilts were the crossbred of Swedish Landrace x Big Yorkshire x German Landrace, selected for reproduction. The serum concentrations of T3 and T4 in four month old gilts (at the beginning of experiment) were normal: 1.48 +/- 0.28 and 46.57 +/- 11.37, respectively. Approximately the same levels of serum thyroid hormones were found in all 3 experimental groups of the gilts at the first oestrus (about 6.5 months) when they were artificially inseminated, at the end of the first third of the pregnancy, at the end of lactation and on the day of first postlactational oestrus. Significantly lower concentrations of serum T4 were only at 1-3 days before parturition, 6-12 hours after parturition and during lactation in all groups of gilts. There were no significant differences in T3 and T4 concentrations as well as in reproductive performances between gilts on the different iodine diet. The authors concluded that 1 p.p.m. of dietary iodine, which is regularly applied in this pig farm, is sufficient to provide normal levels thyroid hormones and normal reproduction. There is not economical and physiological reasons to increase this level of iodine to 2 and 3 p.p.m., since the obtained results showed that these changes don't demonstrated neither positive or negative effects.
Acta Veterinaria-beograd, 2011
The aim of this stady was to examine the relationship between triiodothyronine (T 3), thyroxin (T... more The aim of this stady was to examine the relationship between triiodothyronine (T 3), thyroxin (T 4), insulin-like growth factor-I (IGF-I) and blood serum liver function biomarkers concentrations in Holstein and Red Holstein heifers during the peripartal period. In the first experimental group (I) were heifers of Holstein Friesian breed (n=10), and in the second experimental group (II) were Red Holstein heifers (n=10). Blood samples were taken from the vena coccygea three times during the short peripartal period (5 days before delivery, at delivery and 5 days after delivery), and blood serum samples were separated after spontaneous coagulation. Blood serum T 3 , T 4 and IGF-I concentrations were determinated using radioimmunoassay (RIA). Blood serum concentrations of glucose (G), total protein (TP), albumin, (Alb), urea, total bilirubin (tBil) and aspartate aminotrasferase (AST) were determined using commercial ELISA kits. The statistical significance between means was determined using Student t-test. Blood serum T 3 concentration in Group II was significantly lower after delivery, and T4 concentration was significantly higher before delivery (p<0.05), compared to Group I. Mean blood serum IGF-I concentrations between the two experimental groups were not significantly different at all time intervals. Relationships between the determined hormones showed a median positive correlation between T 3 , and T 4 (r=0.74) in Group I and highly positive correlation in Group II (r=0.81). Blood serum thyroid gland hormones and IGF-I concentrations in healthy heifers during peripartal period vary considerably, the lowest values being determined immediately after calving in both experimental groups. Blood serum G, TP, Alb and urea concentrations were significantly higher in Group I at all investigated time intervals. Blood serum tBil concentration was significantly higher after partus in Group I (11.02 ± 3.33 : 8.49 ± 3.35 mmol/L, Group I vs. II, respectively), and AST activity was not significantly different at all intervals.
PubMed, 1992
The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the se... more The effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the serum levels of thyroid hormones (T3 and T4) has been studied in gilts at various stages of reproductive cycle, as well as on the their reproductive performances. The gilts were the crossbred of Swedish Landrace x Big Yorkshire x German Landrace, selected for reproduction. The serum concentrations of T3 and T4 in four month old gilts (at the beginning of experiment) were normal: 1.48 +/- 0.28 and 46.57 +/- 11.37, respectively. Approximately the same levels of serum thyroid hormones were found in all 3 experimental groups of the gilts at the first oestrus (about 6.5 months) when they were artificially inseminated, at the end of the first third of the pregnancy, at the end of lactation and on the day of first postlactational oestrus. Significantly lower concentrations of serum T4 were only at 1-3 days before parturition, 6-12 hours after parturition and during lactation in all groups of gilts. There were no significant differences in T3 and T4 concentrations as well as in reproductive performances between gilts on the different iodine diet. The authors concluded that 1 p.p.m. of dietary iodine, which is regularly applied in this pig farm, is sufficient to provide normal levels thyroid hormones and normal reproduction. There is not economical and physiological reasons to increase this level of iodine to 2 and 3 p.p.m., since the obtained results showed that these changes don't demonstrated neither positive or negative effects.
Acta Veterinaria-beograd, 2010
The aim of this study was to test a new experimental design of venous system arterialization on t... more The aim of this study was to test a new experimental design of venous system arterialization on the rabbit ear arterialized venous flap (AVF) model. Total number of 10 "Big Chinchila" rabbits were divided in two experimental groups. On both ears of the five rabbits (Group 1) we have performed our original method of venous system arterialization with microsurgical arterialization of the central artery and vein with the preservation of central and peripheral vascular perfusion; at both ears of five rabbits (Group 2) we have performed AVF according to Byan et al., (1995). Vital AVF surface and necrosis percentage were determined in both experimental groups at day 1 and day 14 and results were compared using Student t-test. The results of our experiment indicate that our new experimental design of the AVF on rabbit ear model has better hemodynamic conditions, improves AVF survival and gives significantly bigger vital flap surface at 14 days after venous system arterialization.
Acta Veterinaria-beograd, 2004
The blood plasma concentrations of selenium and plasma and whole blood GSH-Px activities were det... more The blood plasma concentrations of selenium and plasma and whole blood GSH-Px activities were determined in 30 sheep on three farms (A =Ka}, B =E~ka and C=^oka) in marginally deficient areas of Vojvodina. Selenium concentrations in feedstuffs from the territories surrounding the studied farms were lower than 0.10 ppm in more than 90% of the samples. Plasma selenium concentrations in sheep from farms A, B and C were 83.
Journal of Dairy Research
This research paper addresses the hypothesis that an oral supplementation with organically modifi... more This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 ± 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 ± 4 and 4 ± 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at f...
The aim of this work was to determine incidence of calving and postcalving reproduction disorders... more The aim of this work was to determine incidence of calving and postcalving reproduction disorders and their effect on the major parameters of cows reproductive efficiency at dairy farm in the R. Serbia. Total number of 1835 high-milking dairy cows (av. milk production >8500L/305d of lactation) records has been reviewed during one year period, at the large dairy farm in area of Vojvodina (R. Serbia). Normal calving and puerperal period without health problems was observed at 583 cows (27,6%), while different abnormalities during and after calving were recorded in 1252 cows (72,4%). The highest percentages of registered peripartal disorders were dystocia and endometritis (62% and 64%, respectively), affecting almost the same percentage of cows. Peripartal disorders had statistically significant influence (P lt 0.05) on the increase of number of services/conception (3,5 vs. 2,7, reproductive disorders vs. healthy cows, respectively), prolongation of the interval from first postparta...
Acta Veterinaria, 2017
Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormon... more Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual influence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium deficiency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 m...
Acta Veterinaria, 2015
The aim of this investigation was to determine the influence of oxidative stress upon general str... more The aim of this investigation was to determine the influence of oxidative stress upon general stress in dairy cows on parturition and whether the indicators of stress can be reduced by selenium (Se) supplementation.A total of 36 animals were divided into 3 groups 21 days prepartum and treated with a single-term intramuscular supplement of sodium selenite: Control group - 0 mg; group Se10 - 10 mg; group Se20 - 20 mg.Se supplementation significantly raised blood Se content and glutathione peroxidase (GPx) activity in groups Se10 and Se20, compared to Control, although there was no marked difference between supplemented groups. Plasma malondialdehyde (MDA) and cortisol concentrations were significantly reduced in supplemented groups Se10 and Se20, compared to Control. A negative correlation was detected between blood GPx activity and plasma MDA, while a positive correlation was determined between plasma MDA and cortisol concentrations.These results indicate that prepartum Se supplement...
Veterinary Immunology and Immunopathology, 2012
Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' br... more Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc␥ and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecules were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc␥ receptors.
Acta veterinaria, 2006
The involvement of adrenocortical and thyroid hormones in the pathogenesis of ketosis, as well as... more The involvement of adrenocortical and thyroid hormones in the pathogenesis of ketosis, as well as the ovarian consequences of this metabolic disorder, were studied in ³2 parity cows (n=199) in 3 large scale dairy herds. To compare the plasma/serum concentrations of certain hormones Šcortisol, thyroxin (T 4), triiodothyronine (T 3), insulin, insulin-like growth factor-1 (IGF-1)¹ and metabolites Šglucose (G), acetoacetic acid (ACAC), bOH-butyrate (BHB), non-esterified fatty acid (NEFA), trigliceride (TG), total cholesterol (TCh)¹, and the activity of aspartate aminotransferase (AST), blood samples were taken 1 to 3 days after calving and again 4 times 7 days apart. The ACTH-challenged cortisol responsiveness and the TRH-induced T 4 /T 3 increase were determined between days 1 to 3 and again between days 28 to 35. The resumption of ovarian cyclicity was followed up by individual progesterone (P4) profiles based on milk samples taken 3 times a week for about 80 to 85 days. BHB level of 1 mmol/L was estimated as a border line between hyper-(>1 mmol/L) and normoketonaemic (<1 mmol/L) conditions. Five different ketone patterns were distinguished: (1) non-ketotic (n=98; normoketonaemia in all samples), (2) early type ketosis (n=45; hyperketonemia was detected only in the first week after calving), (3) late type (lactational) ketosis (n=11; after a normoketonaemic period increasing hyperketonaemia was detected in the 5 th , or in the 4 th and 5 th weeks), (4) temporary ketosis (n=11; hyperketonaemia was detected for 1-2 weeks in the 2 nd and 3 rd or in the 3 rd and 4 th weeks); (5) long-lasting ketosis (n=34; hyperketonaemia has been detected since calving for 4 to 5 weeks or until dying / emergency slaughtering). Simultaneously with the hyperketonaemic stage increased NEFA, ACAC, depressed TCh, glucose and decreased insulin, IGF-1, T 4 and T 3 concentrations were detected in almost all the cases. Obvious metabolic and endocrine alterations were found, however, only in long-lasting ketosis. The TRH-stimulated T 4 and T 3 responses remained almost unaffected proving intact thyroid function in early and late type as well as in temporary ketosis. Depressed thyroid
Acta veterinaria, 2010
Modern intensive pig production demands an increasing number of insemination doses per ejaculate ... more Modern intensive pig production demands an increasing number of insemination doses per ejaculate of genetically superior boars. In order to achieve such a result the possibility of producing insemination doses in both reduced volume and spermatozoa count without decreasing the fertility of sows is studied. In this trial we studied the effect of insemination with reduced volumes of semen (50 mL) and varied spermatozoa count (4, 2 or 1x10 9). Insemination was performed by the classical (intracervical) or by the new (intrauterine) techniques and the basic fertility parameters (farrowing % and litter size) were measured. The farrowing value decreased with decreasing spermatozoa numbers after intracervical and intrauterine insemination. However, the farrowing value, regardless of spermatozoa numbers, was significantly higher after intrauterine insemination (83.3%, 76.7%, 66.7%) compared to the classical intracervical insemination (73.3%, 66.7% and 50%). Litters size did not vary significantly depending on the applied insemination procedure or spermatozoa number. These results indicate that application of the new intrauterine insemination procedure enables the use of doses with a smaller volume and spermatozoa number, at the same time obtaining a satisfactory farrowing and litter size. This opens the possibility of obtaining a significantly higher number of doses per ejaculate when compared to the classical intracervical insemination procedure. In such a way can be increased significantly the degree of reproductive exploitation of genetically superior boars.
Acta veterinaria, 2012
The aim of this work was to investigate the impact of two energy supplements based on propylene g... more The aim of this work was to investigate the impact of two energy supplements based on propylene glycol in dairy cows diet on ovarian and follicular morphology, conception, insemination index and length of service period. A total number of 60 Holstein Friesian dairy cows, parity between 2-8, with an average milk production of 7000 kg/305 days of lactation were divided into three experimental groups (20 dairy cows per group). The first group of dairy cows was supplemented daily with "Energy-plus" (O1 group; 200 mL propylene-glycol supplement) and the second group was supplemented with "Ketal" (O2 group; 160 mL propylene-glycol supplement), two weeks before partus until 30 days post partum. The third experimental group were non supplemented dairy cows (O3, control group). Ultrasound examination of the reproductive system using real time echo camera Falco VET 100 (ESAOTE PieMedical, Holland, B-shaped scan with linear-array endorectal 5-8 MHz probe) was conducted on every animal starting from day 40 postpartum. The diameters of the ovaries (left and right) and of the dominant follicle(s) were recorded. Ultrasound testing was repeated on day 50 and 60 postpartum only in cows which in the meantime were not inseminated. Reproduction efficiency parameters (conception rate, number of inseminations and length of service period) were recorded individually. The statistical significance of the differences between groups was tested using ANOVA with LSD test at the level of significance p<0.05, chi-square test and Kaplan-Meier survival analysis (the length of service period). There was no significant impact of the propylene glycol supplementation on the ovarian and follicular morphology at the first ultrasound examination. At the second ultrasound examination there was a significant difference between left ovarian dominant follicle diameter in the control and supplemented dairy cows (1.67±0.53 vs 1.12±0.29 and 1.11±0.35 cm, p<0.05, O3 vs O1 and O2, respectively). The cumulative percentage of conception after the first
Acta veterinaria, 2013
The objective of this study was to determine the levels of insulin, insulin like growth factor I ... more The objective of this study was to determine the levels of insulin, insulin like growth factor I (IGF-I) and thyroid hormones in relation to the body condition score (BCS) of periparturient dairy cows. The study was carried out on twenty Holstein-Friesian dairy cows with average milk production of 7000 L/305 days in the previous lactation, parity ranging from 2-4. All cows were BCS scored during the early dry period, 7±3 days before and after parturition. Based on the BCS at the early dry period, cows were divided in two groups: cows with high BCS (3.75-4.25, HBCS, n=10), and cows with moderate BCS (2.75-3.75, MBCS, n=10). Blood samples were taken at the time of BCS evaluation. Concentrations of insulin, IGF-I, triiodothyroinine (T 3) and thyroxine (T 4) were determined by radioimmunoassay (RIA, INEP-Zemun, Serbia). Statistical differences between mean values were determined using Student t-test (p<0.01). Mean BCS values at early dry period were significantly different compared to two other periods (p<0.01) within both groups, but BCS was similar in both groups at 7±3 days after calving (2.69±0.67:2.62± 0.27, p>0.05). IGF-I level in HBCS cows at days 7±3 before calving was significantly higher (16.28±3.07:11.76±2.28, p<0.01), with a reverse relationship after calving (3.77±1.64:8.46±2.37, p<0.01). Insulin level was significantly lower at 7±3 days before calving in HBCS cows (16.26±4.60:20.18±4.96mIU/L, p<0.05). Thyroid hormones levels were significantly lower in HBCS group et all examined periods.
Veterinarski glasnik, 2020
Introduction. In order to provide the most favorable conditions for bone healing following implan... more Introduction. In order to provide the most favorable conditions for bone healing following implant loading, new techniques such as the use of growth factors to directly or indirectly regulate cell and tissue growth are becoming increasingly popular. Simplicity in producing concentrated thrombocytes derived from platelet-rich plasma has led to increasing application of growth factors derived from thrombocytes, such as PDGF, TGF-?, IGF and VEGF, in order to increase the percentage of bone-to-implant contact that provides better implant stability and possibly earlier functional loading. The aim of the study was to determine the effects of platelet-rich plasma and guided tissue regeneration on bone regeneration in the case of bone defects in early dental implant insertion. Materials and Methods. This experimental study was conducted on 10 study dogs that received 40 BCT implants, i.e., 4 implants per dog. The study included early dental implant placement in the sites of the extracted pr...
Research in Veterinary Science, 2016
In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia hav... more In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; viability. The strongest effect of inhibition of cows&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
Animal science papers and reports
The aim of this study was to investigate the relationship between the loss in body condition scor... more The aim of this study was to investigate the relationship between the loss in body condition score (BCS) and loss (ABCS), energy balance (EB), hepatic lipidosis and blood serum concentration of non esterified fatty acids (NEFA), glucose, triacylglycerol (TAG) and total bilirubin (tBIL) in healthy dairy cows during transition period. Twenty healthy Holstein cows were included and categorized into groups based on BCS loss (Delta BCS) between dry period and early lactation (Delta BCS <0.75 and >= 0.75). Significant differences between groups (p<0.05) were observed for blood serum NEFA, glucose and tBIL. Cows with high Delta BCS (>= 0.75) between dry period and early lactation showed increased blood serum NEFA, TAG and tBIL concentrations and lower blood serum glucose concentration
Slovenian Veterinary Research
The objective was to determine the effects of Regumate ® alone or Regumate ® +eCG treatment on th... more The objective was to determine the effects of Regumate ® alone or Regumate ® +eCG treatment on the synchronization of estrus and fertility in delayed puberty (n=30+30) and normal cyclic gilts (n=30+30). Gilts were fed daily a complete diet containing 20 mg/gilt Regumate ® for 18 days. Twenty-four hours after the last feeding of Regumate ® , all gilts received an i.m. injection of 1,000 IU eCG. Gilts were inseminated artificially (AI) 12 and 24 h after first detection of standing estrus. More normal cyclic gilts (84.6%) than delayed puberty gilts (66.7%) expressed estrus by d 4.8 after Regumate ® alone treatment (p < 0.05). However, similar proportion of normal cyclic (90%) and delayed puberty gilts (93%) were in estrus by d 4.2 after Regumate ® + eCG treatment (p > 0.05). These findings indicate that about 70% of delayed puberty gilts had established cyclic ovarian activity (i.e. sexually mature, cyclic gilts), while about 30% were truly delayed puberty gilts (sexually immatur...
Journal of Veterinary Medicine, 2013
The incidence of retained placenta (RP) in cows increases in cases of parturition induced by pros... more The incidence of retained placenta (RP) in cows increases in cases of parturition induced by prostaglandin F2α. We analyzed the effects of different doses of supplemental selenium and vitamin E on the incidence of RP, blood selenium, plasma thyronines, and malondialdehyde concentration. Thirty-three clinically healthy, multiparous Holstein-Frisian cows were assigned to 3 groups and supplemented with a single intramuscular injection of sodium selenite (SS) and tocopherol acetate (TAc) between days 250 to 255 of gestation: control—unsupplemented; group A—10 mg SS + 400 mg TAc; group B—20 mg SS + 800 mg TAc. Parturition was induced using PGF2α not before day 275 of gestation. The RP incidence was reduced from 66.7% in the control to 38.2 and 30.8% in groups A and B, respectively. Blood selenium and glutathione peroxidase activity in treated groups were significantly higher compared to control, with no significant difference between groups A and B. Plasma malondialdehyde in group B was ...
Veterinarski glasnik, 2010
The work presents the results of investigations of the effect of moderate heat stress on the acid... more The work presents the results of investigations of the effect of moderate heat stress on the acidobasal status in high-yield dairy cows in early lactation. Improving performance in high-yield dairy cows increases their inclination toward metabolic disorders. The most likely to be affected is the acid-base balance, in particular when cows are exposed to heat stress. Investigations so far have shown that the taking of urine samples and their analysis is the best and fastest way to diagnose disorders in the acid-base balance. Investigations were carried out on 7 clinically healthy cows of the Holstein- Friesian breed in the phase of early lactation, 30 to 40 days (on days 30, 33 and 40 ) following calving during the summer period, during the month of July, when there were significant variations in daily and nightly temperatures. The cows were in the second and fourth lactation, the annual milk yield was 8000 L milk per cow. The average daily milk production in the early phase of lactat...