Durland Fish - Academia.edu (original) (raw)
Papers by Durland Fish
Parasites & vectors, Jan 9, 2015
Feria-Arroyo et al. had reported previously that, based on PCR analysis, 45 % of Ixodes scapulari... more Feria-Arroyo et al. had reported previously that, based on PCR analysis, 45 % of Ixodes scapularis ticks collected in Texas and Mexico were infected with the Lyme disease spirochete Borrelia burgdorferi (Parasit. Vectors 2014, 7:199). However, our analyses of their initial data (Parasit. Vectors 2014, 7:467) and a recent response by Esteve-Gassent et al. (Parasit. Vectors 2015, 8:129) provide evidence that the positive PCR results obtained from both ribosomal RNA intergenic sequences and the flagellin gene flaB are highly likely due to contamination by the B. burgdorferi B31 positive control strain.
The risk of transmission of West Nile virus (WNV) to humans is associated with the density of inf... more The risk of transmission of West Nile virus (WNV) to humans is associated with the density of infected vector mosquitoes in a given area. Current technology for estimating vector distribution and abundance is primarily based on Centers for Disease Control and Prevention (CDC) light trap collections, which provide only point data. In order to estimate mosquito abundance in areas not
Remote Sensing of Environment, 2008
We provide a method for constructing a county-level West Nile virus risk map to serve as an early... more We provide a method for constructing a county-level West Nile virus risk map to serve as an early warning system for human cases. We also demonstrate that mosquito surveillance is a more accurate predictor of human risk than monitoring dead and infected wild birds.
Oecologia, 2005
Fragmentation of the landscape has been proposed to play an important role in defining local scal... more Fragmentation of the landscape has been proposed to play an important role in defining local scale heterogeneity in Lyme disease risk through influence on mammalian host density and species composition. We tested this observed relationship in a suburban region around Lyme, Connecticut, where we collected data on the density of the tick vector, Ixodes scapularis and prevalence of the Lyme
Cell host & microbe, Jan 15, 2014
Arthopods such as Ixodes scapularis ticks serve as vectors for many human pathogens. The arthropo... more Arthopods such as Ixodes scapularis ticks serve as vectors for many human pathogens. The arthropod gut presents a pivotal microbial entry point and determines pathogen colonization and survival. We show that the gut microbiota of I. scapularis, a major vector of the Lyme disease spirochete Borrelia burgdorferi, influence spirochete colonization of ticks. Perturbing the gut microbiota of larval ticks reduced Borrelia colonization, and dysbiosed larvae displayed decreased expression of the transcription factor signal transducer and activator of transcription (STAT). Diminished STAT expression corresponded to lower expression of peritrophin, a key glycoprotein scaffold of the glycan-rich mucus-like peritrophic matrix (PM) that separates the gut lumen from the epithelium. The integrity of the I. scapularis PM was essential for B. burgdorferi to efficiently colonize the gut epithelium. These data elucidate a functional link between the gut microbiota, STAT-signaling, and pathogen coloniz...
Applied and Environmental Microbiology, 2004
In the northeastern United States, the Lyme disease agent, Borrelia burgdorferi sensu stricto, is... more In the northeastern United States, the Lyme disease agent, Borrelia burgdorferi sensu stricto, is maintained by enzoonotic transmission, cycling between white-footed mice (Peromyscus leucopus) and black-legged ticks (Ixodes scapularis). B. burgdorferi sensu stricto is genetically variable and has been divided into three major genotypes based on 16S-23S ribosomal DNA spacer (RST) analysis. To better understand how genetic differ- ences in
The distribution of human risk for West Nile virus was determined by spatial analysis of the init... more The distribution of human risk for West Nile virus was determined by spatial analysis of the initial case distri- bution for the New York City area in 1999 using remote sensing and geographic information system technologies. Cluster analysis revealed the presence of a statistically significant grouping of cases, which also indicates the area of probable virus introduction. Within the cluster,
Lasers and Electro-Optics Society, IEEE LEOS Annual Meeting, 2003
Among the recent emerging flat display technologies organic LED (OLED) is one of the most interes... more Among the recent emerging flat display technologies organic LED (OLED) is one of the most interesting. Combining the OLED with an active matrix substrate, typically made with low temperature polysilicon, it is possible to greatly reduce the power consumption. In an active matrix substrate the ability of integrating active components can be used to mitigate the OLED differential degradation.
PloS one, 2014
Babesia microti and Borrelia burgdorferi, the respective causative agents of human babesiosis and... more Babesia microti and Borrelia burgdorferi, the respective causative agents of human babesiosis and Lyme disease, are maintained in their enzootic cycles by the blacklegged tick (Ixodes scapularis) and use the white-footed mouse (Peromyscus leucopus) as primary reservoir host. The geographic range of both pathogens has expanded in the United States, but the spread of babesiosis has lagged behind that of Lyme disease. Several studies have estimated the basic reproduction number (R0) for B. microti to be below the threshold for persistence (<1), a finding that is inconsistent with the persistence and geographic expansion of this pathogen. We tested the hypothesis that host coinfection with B. burgdorferi increases the likelihood of B. microti transmission and establishment in new areas. We fed I. scapularis larva on P. leucopus mice that had been infected in the laboratory with B. microti and/or B. burgdorferi. We observed that coinfection in mice increases the frequency of B. microt...
Ticks and Tick-borne Diseases, 2014
Vector-Borne and Zoonotic Diseases, 2006
Vector-Borne and Zoonotic Diseases, 2009
This series of articles describes the first large-scale experiment designed to explore the effica... more This series of articles describes the first large-scale experiment designed to explore the efficacy of reducing the risk of tick-borne disease in highly endemic communities of the northeastern and Mid-Atlantic United States through deployment of a self-application device that treats white-tailed deer with acaricide to prevent feeding by adult Ixodes scapularis ticks and all stages of Amblyomma americanum ticks where both species occur. The results of the multicenter study are reported in the accompanying articles in this issue. This article describes the background and rationale for this experiment by reviewing relevant literature on current tick-borne disease epidemics and previous efforts to reduce the public health burden of Lyme disease and other tick-borne diseases.
Vector-Borne and Zoonotic Diseases, 2009
Vector-Borne and Zoonotic Diseases, 2008
Proceedings of the National Academy of Sciences, 2004
Proceedings of the National Academy of Sciences, 2008
Parasites & Vectors, 2010
Vector borne and zoonotic diseases (Larchmont, N.Y.), 2002
The distribution of human risk for West Nile virus was determined by spatial analysis of the init... more The distribution of human risk for West Nile virus was determined by spatial analysis of the initial case distribution for the New York City area in 1999 using remote sensing and geographic information system technologies. Cluster analysis revealed the presence of a statistically significant grouping of cases, which also indicates the area of probable virus introduction. Within the cluster, habitat suitability for potentially infective adult mosquitoes was measured by the amount of vegetation cover using satellite imagery. Logistic regression analysis revealed satellite-derived vegetation abundance to be significantly and positively associated with the presence of human cases. The logistic model was used to estimate the spatial distribution of human risk for West Nile virus throughout New York City. Accuracy of the resulting risk map was cross-validated using virus-positive mosquito sample sites. These new epidemiological methods aid in rapid entry point identification and spatial p...
Parasites & vectors, Jan 9, 2015
Feria-Arroyo et al. had reported previously that, based on PCR analysis, 45 % of Ixodes scapulari... more Feria-Arroyo et al. had reported previously that, based on PCR analysis, 45 % of Ixodes scapularis ticks collected in Texas and Mexico were infected with the Lyme disease spirochete Borrelia burgdorferi (Parasit. Vectors 2014, 7:199). However, our analyses of their initial data (Parasit. Vectors 2014, 7:467) and a recent response by Esteve-Gassent et al. (Parasit. Vectors 2015, 8:129) provide evidence that the positive PCR results obtained from both ribosomal RNA intergenic sequences and the flagellin gene flaB are highly likely due to contamination by the B. burgdorferi B31 positive control strain.
The risk of transmission of West Nile virus (WNV) to humans is associated with the density of inf... more The risk of transmission of West Nile virus (WNV) to humans is associated with the density of infected vector mosquitoes in a given area. Current technology for estimating vector distribution and abundance is primarily based on Centers for Disease Control and Prevention (CDC) light trap collections, which provide only point data. In order to estimate mosquito abundance in areas not
Remote Sensing of Environment, 2008
We provide a method for constructing a county-level West Nile virus risk map to serve as an early... more We provide a method for constructing a county-level West Nile virus risk map to serve as an early warning system for human cases. We also demonstrate that mosquito surveillance is a more accurate predictor of human risk than monitoring dead and infected wild birds.
Oecologia, 2005
Fragmentation of the landscape has been proposed to play an important role in defining local scal... more Fragmentation of the landscape has been proposed to play an important role in defining local scale heterogeneity in Lyme disease risk through influence on mammalian host density and species composition. We tested this observed relationship in a suburban region around Lyme, Connecticut, where we collected data on the density of the tick vector, Ixodes scapularis and prevalence of the Lyme
Cell host & microbe, Jan 15, 2014
Arthopods such as Ixodes scapularis ticks serve as vectors for many human pathogens. The arthropo... more Arthopods such as Ixodes scapularis ticks serve as vectors for many human pathogens. The arthropod gut presents a pivotal microbial entry point and determines pathogen colonization and survival. We show that the gut microbiota of I. scapularis, a major vector of the Lyme disease spirochete Borrelia burgdorferi, influence spirochete colonization of ticks. Perturbing the gut microbiota of larval ticks reduced Borrelia colonization, and dysbiosed larvae displayed decreased expression of the transcription factor signal transducer and activator of transcription (STAT). Diminished STAT expression corresponded to lower expression of peritrophin, a key glycoprotein scaffold of the glycan-rich mucus-like peritrophic matrix (PM) that separates the gut lumen from the epithelium. The integrity of the I. scapularis PM was essential for B. burgdorferi to efficiently colonize the gut epithelium. These data elucidate a functional link between the gut microbiota, STAT-signaling, and pathogen coloniz...
Applied and Environmental Microbiology, 2004
In the northeastern United States, the Lyme disease agent, Borrelia burgdorferi sensu stricto, is... more In the northeastern United States, the Lyme disease agent, Borrelia burgdorferi sensu stricto, is maintained by enzoonotic transmission, cycling between white-footed mice (Peromyscus leucopus) and black-legged ticks (Ixodes scapularis). B. burgdorferi sensu stricto is genetically variable and has been divided into three major genotypes based on 16S-23S ribosomal DNA spacer (RST) analysis. To better understand how genetic differ- ences in
The distribution of human risk for West Nile virus was determined by spatial analysis of the init... more The distribution of human risk for West Nile virus was determined by spatial analysis of the initial case distri- bution for the New York City area in 1999 using remote sensing and geographic information system technologies. Cluster analysis revealed the presence of a statistically significant grouping of cases, which also indicates the area of probable virus introduction. Within the cluster,
Lasers and Electro-Optics Society, IEEE LEOS Annual Meeting, 2003
Among the recent emerging flat display technologies organic LED (OLED) is one of the most interes... more Among the recent emerging flat display technologies organic LED (OLED) is one of the most interesting. Combining the OLED with an active matrix substrate, typically made with low temperature polysilicon, it is possible to greatly reduce the power consumption. In an active matrix substrate the ability of integrating active components can be used to mitigate the OLED differential degradation.
PloS one, 2014
Babesia microti and Borrelia burgdorferi, the respective causative agents of human babesiosis and... more Babesia microti and Borrelia burgdorferi, the respective causative agents of human babesiosis and Lyme disease, are maintained in their enzootic cycles by the blacklegged tick (Ixodes scapularis) and use the white-footed mouse (Peromyscus leucopus) as primary reservoir host. The geographic range of both pathogens has expanded in the United States, but the spread of babesiosis has lagged behind that of Lyme disease. Several studies have estimated the basic reproduction number (R0) for B. microti to be below the threshold for persistence (<1), a finding that is inconsistent with the persistence and geographic expansion of this pathogen. We tested the hypothesis that host coinfection with B. burgdorferi increases the likelihood of B. microti transmission and establishment in new areas. We fed I. scapularis larva on P. leucopus mice that had been infected in the laboratory with B. microti and/or B. burgdorferi. We observed that coinfection in mice increases the frequency of B. microt...
Ticks and Tick-borne Diseases, 2014
Vector-Borne and Zoonotic Diseases, 2006
Vector-Borne and Zoonotic Diseases, 2009
This series of articles describes the first large-scale experiment designed to explore the effica... more This series of articles describes the first large-scale experiment designed to explore the efficacy of reducing the risk of tick-borne disease in highly endemic communities of the northeastern and Mid-Atlantic United States through deployment of a self-application device that treats white-tailed deer with acaricide to prevent feeding by adult Ixodes scapularis ticks and all stages of Amblyomma americanum ticks where both species occur. The results of the multicenter study are reported in the accompanying articles in this issue. This article describes the background and rationale for this experiment by reviewing relevant literature on current tick-borne disease epidemics and previous efforts to reduce the public health burden of Lyme disease and other tick-borne diseases.
Vector-Borne and Zoonotic Diseases, 2009
Vector-Borne and Zoonotic Diseases, 2008
Proceedings of the National Academy of Sciences, 2004
Proceedings of the National Academy of Sciences, 2008
Parasites & Vectors, 2010
Vector borne and zoonotic diseases (Larchmont, N.Y.), 2002
The distribution of human risk for West Nile virus was determined by spatial analysis of the init... more The distribution of human risk for West Nile virus was determined by spatial analysis of the initial case distribution for the New York City area in 1999 using remote sensing and geographic information system technologies. Cluster analysis revealed the presence of a statistically significant grouping of cases, which also indicates the area of probable virus introduction. Within the cluster, habitat suitability for potentially infective adult mosquitoes was measured by the amount of vegetation cover using satellite imagery. Logistic regression analysis revealed satellite-derived vegetation abundance to be significantly and positively associated with the presence of human cases. The logistic model was used to estimate the spatial distribution of human risk for West Nile virus throughout New York City. Accuracy of the resulting risk map was cross-validated using virus-positive mosquito sample sites. These new epidemiological methods aid in rapid entry point identification and spatial p...