Durna Aliyeva - Academia.edu (original) (raw)

Papers by Durna Aliyeva

Zenodo (CERN European Organization for Nuclear Research), Oct 25, 2015

Abşeron ərazisinə təşkil edilən fitopatoloji monitorinqlər zamanı xarakterik tabomovirus əlamətlə... more Abşeron ərazisinə təşkil edilən fitopatoloji monitorinqlər zamanı xarakterik tabomovirus əlamətlərinə malik tomat bitkiləri aşkar edilmişdir. Toplanmış nümunələrdən total RNT ayrılmış və tobamoviruslar üçün universal olan tobamo 1 və tobamo 2 praymer cütlükəri ilə aparılan RT-PCR metodu və serioloji analizlərlə simptomatik bitkilərin tobamoviruslarla yoluxması təsdiq edilmişdir. Eyni zamanda, sağlam və xəstə bitkilərin yarpaqlarında müqayisəli şəkildə malondialdehidin miqdarı, qvayakol peroksidaza (QPO) və benzidin (BPO) fermentlərinin fəallığı və izoferment tərkibi tədqiq edilmişdir. Müəyyən edilmişdir ki, patogenez zamanı malondialdehidin miqdarı artır, peroksidazaların fəallığı yüksəlir və onların molekulyar formalarında kəmiyyət və keyfiyyət dəyişiklikləri baş verir.

Zenodo (CERN European Organization for Nuclear Research), Sep 30, 2013

Устойчивость растений к засухе обеспечивается многокомпонентной антиоксидантной системой, вызываю... more Устойчивость растений к засухе обеспечивается многокомпонентной антиоксидантной системой, вызывающей перестройки на физиологическом, клеточном и молекулярном уровне. В данной работе изучено влияние почвенной засухи на изменение активности и субклеточной локализации изоформ супероксиддисмутазы, уровни малонового диальдегида, глицин бетаина, а также содержание общих белков у двух контрастных генотипов твердой пшеницы (Triticum durum Desf.). Обнаружена зависимость уровня активности ферментов от генотипа пшеницы, продолжительности засухи и этапов развития растений. Реакция растений пшеницы на водный дефицит проявляется в накоплении глицинбетаина в листьях. При усилении засухи у неустойчивого генотипа Гарагылчыг-2 в отличие от устойчивого Баракатли-95, происходило значительное накопление МДА. Нативный электрофорез на полиакриламидном геле показал наличие 9 изоформ супероксиддисмутазы в листьях пшеницы во время засухи. Листья пшеницы содержат три различных типа СОД (Mn-, Fe-, Cu/Zn-СОД). Mn-СОД был обнаружен в митохондриальных фракциях, Fe-СОД-в хлоропластных фракциях, а Cu/Zn-СОД локализуется во всех субклеточных фракциях. Долговременная почвенная засуха сопровождалась экспрессией уровней этих изоформ. С использованием ингибиторного анализа (3мM KCN и 5 мМ Н 2 О 2) в листьях пшеницы наблюдалось три изоформы Mn-СОД, одна изоформа Fe-СОД и пять изоформ Cu/Zn-СОД. Установлено, что в растениях подвергнутых водному стрессу в повышение активности СОД наибольший вклад вносит Mn-СОД. Вероятно, основная роль в детоксикации супероксид радикала во время долговременной почвенной засухи принадлежит этой изоформе.

Zenodo (CERN European Organization for Nuclear Research), Oct 8, 2012

Biochemical Society Transactions, 2000

The strategically important wheat plant is the most cultivated of cereals. Drought is one of the ... more The strategically important wheat plant is the most cultivated of cereals. Drought is one of the main factors adversely affecting its productivity and quality of grain. Components of the ascorbate-glutathione cycle play an important role in the antioxidant defence system. The diurnal dynamics of temperature-related glutathione reductase activity (GR), one of the main components of the antioxidant defence system, were studied in durum (Barakatli 95, Garagylchyg 2) and bread (Gobustan, Tale 38) wheat genotypes of contrasting tolerance, when exposed to sustained soil drought. Glutathione reductase is highly sensitive to glutathione. Leaf samples were taken at the end of the wax ripening phase at three-hourly intervals (800, 1100, 1400, 1700), frozen in liquid N2, and kept at -800C. The experiments indicated that GR activity increased in stressed, tolerant, (Barakatli 95, Gobustan) and decreased in stressed sensitive varieties (Garagylchyg 2, Tale 38) compared with watered variants. In ...

Visnyk of Dnipropetrovsk University. Biology, medicine, 2010

The effect of elevated NaCl concentrations (0.5–4.0 M) on the proline content and activity of fre... more The effect of elevated NaCl concentrations (0.5–4.0 M) on the proline content and activity of free peroxidase in green alga Dunaliella salina have been investigated. It was revealed that the tolerance of the alga to NaCl effect was determined by the functioning of the complex of metabolic reactions including both the necessary stress-induced accumulation of proline and high activity of the free peroxidase. The obtained data demonstrate testify to a deep reorganization of metabolic processes, occurring in alga cells during the adaptation to elevated salinity conditions.

Photosynthetica, 2017

Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to eval... more Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to evaluate the incidence of main Tobamoviruses. According to results of serological and molecular tests, Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), and Pepper mild mottle virus (PMMoV) were detected as single and mixed infections (TMV + PMMoV; ToMV + PMMoV) in various tomato samples. It was found that Tobamovirus infection caused an increase in the content of malondialdehyde, alterations in the activities of peroxidase enzymes and quantitative and qualitative changes in their molecular isoforms. A comparison of thylakoid membrane polypeptides from virus-infected leaves indicated a decrease in the content of the thylakoid membrane polypeptides with molecular masses of 123, 55, 47, 33, 28-24, 17, and 15 kD. PSII efficiency and the content of chlorophylls (a and b) were significantly lower in the virus-infected leaves.

Indian Journal of Science and Technology, 2021

Objective: Grapevine (Vitis vinifera L.) is one of the major crops widely cultivated for the wine... more Objective: Grapevine (Vitis vinifera L.) is one of the major crops widely cultivated for the wine industry, as well as for the production of fresh and dried fruit in Azerbaijan. Grapevine leafroll disease (GLD) affects the vines throughout the world and is considered the most economically destructive among all virus and virus-like diseases. Vineyard surveys were conducted to determine the virus infection in the major grapevine growing region of Azerbaijan during 2019-2020. Methods/Statistical analysis: Forty-six samples were collected from grapevine fields and screened by rapid one-step assay AgriStrip and double-antibody sandwich Enzyme-linked immunosorbent assay (DAS-ELISA). In our study, the levels of malondialdehyde (MDA), hydrogen peroxide (H 2 O 2), pigments, relative water content (RWC), alterations in the activities of peroxidase enzymes (the activities of ascorbate peroxidase (APO), benzidine peroxidase (BPO) and guaiacol peroxidase (GPO) were investigated. Findings: The results revealed that tested samples were infected with Grapevine leafrollassociated virus 3 (GLRaV-3), however, no sample was found infected with other Grapevine leafroll-associated viruses. Changes in the levels of MDA, H 2 O 2 , pigments, RWC, alterations in the activities of peroxidase enzymes were studied. Significant reduction in green pigments like chlorophylls (a, b and total) and a gradual reduction in carotenoids were observed in all infected species. Obtained results showed that the level of RWC and the amount of hydrogen peroxide had increased in all infected leaves. The activities of APO, BPO and GPO were observed to increase in virus-infected leaves compared to the healthy control. Based on the results, it can be concluded that the effect of GLRaV-3 was more destructive in the N2 sample. The antioxidant defense system works more effectively in the N5 sample and this plant is more resistant to viral infection. Novelty: These results indicate that GLRaV-3 was the only most prevalent endemic viral pathogen of grapevine in the dominantly warm

Photosynthetica, 2020

Land plants shorten their vegetation period during water deficiency. Under water stress, wheat pl... more Land plants shorten their vegetation period during water deficiency. Under water stress, wheat plants undergo several changes at the morphophysiological level. We tried to elucidate the role of the morphophysiological parameters and the photosynthetic machinery in response to drought followed by rewatering. Therefore, some morphophysiological traits of two bread wheat genotypes (drought-tolerant Gobustan, drought-sensitive Tale 38) were studied. The H2O2 content increased under drought in both genotypes but recovered in the Gobustan genotype after rewatering. The isozymes of peroxidase manifested dynamic changes under drought. The electron transport rate and the maximum photochemical quantum efficiency of PSII showed similar responses to drought with subsequent rewatering in both genotypes. However, the amount of the photosynthetic pigments changed drastically resulting in structural changes of thylakoid membranes. In Gobustan, the thylakoid membrane structure almost completely recovered after rewatering. Thus, the drought-tolerant genotype shows a more dynamic response of photosynthetic machinery and antioxidant capacity.

Functional Plant Biology, 2020

A characteristic feature of C4 plants is the differentiation of the photosynthetic leaf tissues i... more A characteristic feature of C4 plants is the differentiation of the photosynthetic leaf tissues into two distinct cell types: mesophyll (M) and bundle sheath (BS) cells. We have investigated several biochemical parameters, including pigment composition, polypeptide patterns, fluorescence at 77K, the activity of photosystems and ultrastructure of mesophyll and bundle sheath chloroplasts of maize (Zea mays L.) plants. It is shown that the BS chloroplasts have ~2-fold higher chlorophyll a/b ratio than M chloroplasts, 6.15 and 3.12 respectively. The PSI apoprotein (68 kDa) was more abundant in BS than in M thylakoids. Polypeptides belonging to PSII core antenna, are in similar amounts in both types of membranes, but the 45kDa band is more intensive in M thylakoids. Polypeptides in the region of 28–24 kDa of the light-harvesting complex of PSII (LHCII) are also present in both types of chloroplasts, though their amounts are reduced in BS thylakoids. The chlorophyll fluorescence emission ...

Net Journal of Agricultural Science, 2017

Photosynthesis Research, 2016

Water deficit is a key factor influencing the yield and quality of crops. In the present study, t... more Water deficit is a key factor influencing the yield and quality of crops. In the present study, the photosynthetic responses by means of chlorophyll fluorescence of chloroplasts, thylakoid membrane proteins, and antioxidant components were analyzed in wheat (Triticum durum Desf.) plants differing in their tolerance to drought. Two durum winter wheat varieties, Barakatli 95 (drought tolerant) and Garagylchyg 2 (drought sensitive) were grown under field well-watered and drought conditions. It was found that contents of the PS I core (CPI) with Mr of 123 kD and apoprotein P700 with Mr of 63 kD were relatively higher in Barakatli 95 variety under drought stress compared with the control plants. Synthesis of aand b-subunits of CF 1 ATP-synthase complex with Mr of 55 and 53.5 kD also slightly increased in the tolerant Barakatli 95 and decreased in the drought sensitive variety Garagylchyg 2. A decrease in the intensity of 30 kD band and a significant increase were found in the content of the 25-16 kD region in Garagylchyg 2 variety. The synthesis of 60 kD and content of low molecular mass polypeptides (21.5 and 12 kD) were increased in the tolerant genotype Barakatli 95. The intensity of peaks at 687, 695, and 742 nm considerably increases in the fluorescence spectra (77 K) of chloroplasts isolated from the sensitive variety Garagylchyg 2, and there is a stimulation of the ratio of fluorescence band intensity F687/F740. At the same time, higher level of glycine betaine was found in the drought tolerant variety compared with the control one throughout the different periods of growth.

Photosynthesis Research, 2015

The dynamics of the activity of catalase, ascorbate peroxidase, guaiacol peroxidase, and benzidin... more The dynamics of the activity of catalase, ascorbate peroxidase, guaiacol peroxidase, and benzidine peroxidase, as well as the level of hydrogen peroxide in the vegetative organs of durum wheat (Triticum durum Desf.) cultivars was studied under long-term soil drought conditions. It was established that hydrogen peroxide generation occurred at early stages of stress in the tolerant variety Barakatli-95, whereas in the susceptible variety Garagylchyg-2 its significant amounts were accumulated only at later stages. Garagylchyg-2 shows a larger reduction of photochemical activity of PS II in both genotypes at all stages of ontogenesis under drought stress than Barakatli-95. The highest activity of catalase which plays a leading role in the neutralization of hydrogen peroxide was observed in the leaves and roots of the drought-tolerant variety Barakatli-95. Despite the fact that the protection system also includes peroxidases, the activity of these enzymes even after synthesis of their new portions is substantially lower compared with catalase. Native PAGE electrophoresis revealed the presence of one isoform of CAT, seven isoforms of APX, three isoforms of GPO, and three isoforms of BPO in the leaves, and also three isoforms of CAT, four isoforms of APX, two isoforms of GPO, and six isoforms of BPO in the roots of wheat. One isoform of CAT was found in the roots when water supply was normal and three isoforms were observed under drought conditions. Stress associated with long-term soil drought in the roots of wheat has led to an increase in the heterogeneity due to the formation of two new sedentary forms of catalase: CAT2 and CAT3.

Biosystems Diversity, 2009

Исследовано влияние повышение концентрации NaCl (от 0,5 до 4,0 м) на пигментный состав, кислородн... more Исследовано влияние повышение концентрации NaCl (от 0,5 до 4,0 м) на пигментный состав, кислородный обмен и активность каталазы клеток зеленой водоросли Dunaliella salina. Установлено оптимальную концентрацию NaCl (2,0 М), при которой отмечено интенсивное биосинтез зеленых пигментов и функционирования фотосинтетического аппарата. При повышенных концентрациях NaCl (3,0 и 4,0 м) растет активность каталазы в 5,8 раза в пересчете на 1 мг белка по сравнению с контролем, что составляло 0,5703 мкмоль / мин. на мг белка. Активность каталазы может быть использована как показатель устойчивости клеток водоросли в условиях солевого стресса

Plant Physiology and Biochemistry, 2014

Water is a key factor influencing the yield and quality of crops. One of the parameters of plant ... more Water is a key factor influencing the yield and quality of crops. One of the parameters of plant biological tolerance to constantly changing environmental conditions is the change of activities and numerous molecular forms of antioxidant enzymes. Two durum (Triticum durum Desf.) wheat varieties contrasting for drought tolerance, such as Barakatli-95 (drought tolerant) and Garagylchyg-2 (drought sensitive) were grown over a wide area in the field. Experiments were carried out to study the effect of soil drought on changes in activities and subcellular localization of superoxide dismutase isoforms. The levels of malondialdehyde, glycine betaine and total proteins were also analyzed. The level of the enzyme activity appeared to depend on the wheat varieties, duration of drought and stages of leaf development. Native polyacrylamide gel electrophoresis (PAGE) revealed the presence of 9 isoenzymes of superoxide dismutase in wheat leaves during drought. Mn-SOD was found in the mitochondrial fractions, Fe-SOD in the chloroplast fraction and Cu/Zn-SOD is localized in all subcellular fractions. Wheat leaves contain three different isoforms of SOD (Mn-, Fe-, Cu/Zn-SOD). Three isoforms of Mn-SOD, one isoform of Fe-SOD and five of Cu/Zn-SOD were observed in wheat leaves using 3 mM KCN and 5 mM H2O2 as selective inhibitors. The expression of Mn-SOD was preferentially enhanced by drought stress. It seems that Mn-SOD isoforms more than SOD ones play a major role in the scavenging of superoxide radicals. The observed data showed that status of antioxidant enzymes such as SOD could provide a meaningful tool for depicting drought tolerance of wheat genotype.

A Sustainable Approach, 2016

Drought Stress Tolerance in Plants, Vol 1, 2016

Drought tolerance is considered to be a quantitative trait manifesting complex phenotypic and gen... more Drought tolerance is considered to be a quantitative trait manifesting complex phenotypic and genetic control (McWilliam 1989; Saleh et al. 2014). Because of global environmental changes and increase in the world population, maintaining plant productivity under drought conditions is of great importance (Takeda and Matsuoka 2008). Interest in research of physiological and biochemical processes improving plant tolerance against adverse environmental factors has been increasing recently (Bray et al. 2000; Wang et al. 2003). From this point of view the study of stress effects on physiological and biochemical processes occurring in higher plants is considered to be actual.

Functional Plant Biology

The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H2O2 scavenging in plants. The ef... more The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H2O2 scavenging in plants. The effect of diurnal variations in hydrogen peroxide (H2O2) content, the intensity of lipid peroxidation (malondialdehyde, MDA), photosynthesis, antioxidants and antioxidative enzyme activities involved in AsA-GSH metabolism has been studied comparatively in leaves of durum (Triticum durum Desf.) and bread (Triticum aestivum L.) wheat genotypes exposed to soil drought. Drought stress caused an increase in the content of H2O2, MDA, alterations in the activities of AsA-GSH cycle enzymes and quantitative changes in AsA and GSH content during the day. PSII efficiency was significantly lower in the control and drought exposed leaves at the highest temperature in the afternoon. The ascorbate peroxidase activity was found to increase and ascorbic acid amount decreased with increasing temperature during the day. Further, the glutathione amount and glutathione reductase activity increased at the expen...

Physiological and Molecular Plant Pathology