Esad Dzajic - Academia.edu (original) (raw)

Papers by Esad Dzajic

Research paper thumbnail of Investigation of possible clonal transmission of carbapenemase-producing Klebsiella pneumoniae complex member isolates in Denmark using core genome MLST and National Patient Registry Data

International Journal of Antimicrobial Agents, 2020

Research paper thumbnail of Community-acquired meningitis caused by beta-haemolytic streptococci in adults: a nationwide population-based cohort study

European Journal of Clinical Microbiology & Infectious Diseases, 2019

Research paper thumbnail of Azole resistance in Aspergillus fumigatus. The first 2‐year's Data from the Danish National Surveillance Study, 2018–2020

Mycoses, 2022

BackgroundAzole resistance complicates treatment of patients with invasive aspergillosis with an ... more BackgroundAzole resistance complicates treatment of patients with invasive aspergillosis with an increased mortality. Azole resistance in Aspergillus fumigatus is a growing problem and associated with human and environmental azole use. Denmark has a considerable and highly efficient agricultural sector. Following reports on environmental azole resistance in A. fumigatus from Danish patients, the ministry of health requested a prospective national surveillance of azole‐resistant A. fumigatus and particularly that of environmental origin.ObjectivesTo present the data from the first 2 years of the surveillance programme.MethodsUnique isolates regarded as clinically relevant and any A. fumigatus isolated on a preferred weekday (background samples) were included. EUCAST susceptibility testing was performed and azole‐resistant isolates underwent cyp51A gene sequencing.ResultsThe azole resistance prevalence was 6.1% (66/1083) at patient level. The TR34/L98H prevalence was 3.6% (39/1083) an...

Research paper thumbnail of A decade of nation-wide fungaemia surveillance in Denmark: Current status and major trends

M.C. Arendrup1, R.H. Jensen1, H.K. Johansen2, J.D. Knudsen3, L.E. Lemming4, B.L. Røder5, F.S. Ros... more M.C. Arendrup1, R.H. Jensen1, H.K. Johansen2, J.D. Knudsen3, L.E. Lemming4, B.L. Røder5, F.S. Rosenvinge6, L. Kristensen7, L. Nielsen8, B. Olesen8, E. Dzajic9, P. Kjældgård10, H.C. Schønheyder11 1Statens Serum Institut, Copenhagen, Denmark 2Rigshospitalet, Copenhagen, Denmark 3Hvidovre University Hospital, Hvidovre, Denmark 4Århus University Hospital, Århus, Denmark 5Slagelse Sygehus, Slagelse, Denmark 6Vejle Sygehus, Vejle, Denmark 7Herning Centralsygehus, Herning, Denmark 8Herlev University Hospital, Herlev, Denmark 9Esbjerg Sygehus, Esbjerg, Denmark 10Sønderborg Hospital, Sønderborg, Denmark 11Ålborg University Hospital, Alborg, Denmark

Research paper thumbnail of P08.37 Epidemiological trends in chlamydia testing in denmark 1991 to 2011 and formation of a retrospective, population-based cohort: the danish chlamydia study

Sexually Transmitted Infections, 2015

Introduction Despite intensive efforts Chlamydia trachomatis (Ct) remains the most common bacteri... more Introduction Despite intensive efforts Chlamydia trachomatis (Ct) remains the most common bacterial sexually transmitted infection worldwide. Therefore, to gain more insight into the epidemiology and transmission of Ct, multilocus sequence typing (MLST) schemes have been developed. However, there is no consensus regarding the use of an MLST scheme for epidemiological studies. Therefore, the objective of this study was to investigate the clustering of Ct strains using two MLST schemes with differing discriminatory capacities. Methods We selected and tested samples from Ct infected men having sex with man (MSM) and heterosexual women using two existing MLST schemes. One MLST scheme was based on 6 highly variable targets in the chlamydial genome, also referred to as high-resolution MLST (hr-MLST-6) and the other scheme was based on 7 housekeeping genes (MLST-7). For this study, the existing MLST-7 scheme was modified to a nested PCR enabling to test clinical samples. Sequences obtained from both MLST schemes were analysed for cluster analysis and phylogenetic relationships. Results Full profiles were obtained for 175 samples; 84 (48%) were from MSM and 91 (52%) from heterosexuals. We detected 32 sequence types (STs) using the MLST-7scheme and 78 STs using the hr-MLST-6scheme. Cluster analysis using hr-MLST-6data showed segregation between isolates from MSM and heterosexuals. However, cluster analysis using the MLST-7 data did not show this segregation between isolates from MSM and heterosexuals. Conclusion The MLST-7 scheme was not able to discern closely related Ct strains over a small time-frame confirming that it is more suitable for evolutionary studies. For short-term cluster analysis the hr-MLST-6scheme is considered more suitable to study the epidemiology and transmission of Ct in various populations.

Research paper thumbnail of Investigation of possible clonal transmission of carbapenemase-producing Klebsiella pneumoniae complex member isolates in Denmark using core genome MLST and National Patient Registry Data

International Journal of Antimicrobial Agents, 2020

Research paper thumbnail of Community-acquired meningitis caused by beta-haemolytic streptococci in adults: a nationwide population-based cohort study

European Journal of Clinical Microbiology & Infectious Diseases, 2019

Research paper thumbnail of Azole resistance in Aspergillus fumigatus. The first 2‐year's Data from the Danish National Surveillance Study, 2018–2020

Mycoses, 2022

BackgroundAzole resistance complicates treatment of patients with invasive aspergillosis with an ... more BackgroundAzole resistance complicates treatment of patients with invasive aspergillosis with an increased mortality. Azole resistance in Aspergillus fumigatus is a growing problem and associated with human and environmental azole use. Denmark has a considerable and highly efficient agricultural sector. Following reports on environmental azole resistance in A. fumigatus from Danish patients, the ministry of health requested a prospective national surveillance of azole‐resistant A. fumigatus and particularly that of environmental origin.ObjectivesTo present the data from the first 2 years of the surveillance programme.MethodsUnique isolates regarded as clinically relevant and any A. fumigatus isolated on a preferred weekday (background samples) were included. EUCAST susceptibility testing was performed and azole‐resistant isolates underwent cyp51A gene sequencing.ResultsThe azole resistance prevalence was 6.1% (66/1083) at patient level. The TR34/L98H prevalence was 3.6% (39/1083) an...

Research paper thumbnail of A decade of nation-wide fungaemia surveillance in Denmark: Current status and major trends

M.C. Arendrup1, R.H. Jensen1, H.K. Johansen2, J.D. Knudsen3, L.E. Lemming4, B.L. Røder5, F.S. Ros... more M.C. Arendrup1, R.H. Jensen1, H.K. Johansen2, J.D. Knudsen3, L.E. Lemming4, B.L. Røder5, F.S. Rosenvinge6, L. Kristensen7, L. Nielsen8, B. Olesen8, E. Dzajic9, P. Kjældgård10, H.C. Schønheyder11 1Statens Serum Institut, Copenhagen, Denmark 2Rigshospitalet, Copenhagen, Denmark 3Hvidovre University Hospital, Hvidovre, Denmark 4Århus University Hospital, Århus, Denmark 5Slagelse Sygehus, Slagelse, Denmark 6Vejle Sygehus, Vejle, Denmark 7Herning Centralsygehus, Herning, Denmark 8Herlev University Hospital, Herlev, Denmark 9Esbjerg Sygehus, Esbjerg, Denmark 10Sønderborg Hospital, Sønderborg, Denmark 11Ålborg University Hospital, Alborg, Denmark

Research paper thumbnail of P08.37 Epidemiological trends in chlamydia testing in denmark 1991 to 2011 and formation of a retrospective, population-based cohort: the danish chlamydia study

Sexually Transmitted Infections, 2015

Introduction Despite intensive efforts Chlamydia trachomatis (Ct) remains the most common bacteri... more Introduction Despite intensive efforts Chlamydia trachomatis (Ct) remains the most common bacterial sexually transmitted infection worldwide. Therefore, to gain more insight into the epidemiology and transmission of Ct, multilocus sequence typing (MLST) schemes have been developed. However, there is no consensus regarding the use of an MLST scheme for epidemiological studies. Therefore, the objective of this study was to investigate the clustering of Ct strains using two MLST schemes with differing discriminatory capacities. Methods We selected and tested samples from Ct infected men having sex with man (MSM) and heterosexual women using two existing MLST schemes. One MLST scheme was based on 6 highly variable targets in the chlamydial genome, also referred to as high-resolution MLST (hr-MLST-6) and the other scheme was based on 7 housekeeping genes (MLST-7). For this study, the existing MLST-7 scheme was modified to a nested PCR enabling to test clinical samples. Sequences obtained from both MLST schemes were analysed for cluster analysis and phylogenetic relationships. Results Full profiles were obtained for 175 samples; 84 (48%) were from MSM and 91 (52%) from heterosexuals. We detected 32 sequence types (STs) using the MLST-7scheme and 78 STs using the hr-MLST-6scheme. Cluster analysis using hr-MLST-6data showed segregation between isolates from MSM and heterosexuals. However, cluster analysis using the MLST-7 data did not show this segregation between isolates from MSM and heterosexuals. Conclusion The MLST-7 scheme was not able to discern closely related Ct strains over a small time-frame confirming that it is more suitable for evolutionary studies. For short-term cluster analysis the hr-MLST-6scheme is considered more suitable to study the epidemiology and transmission of Ct in various populations.