ELZA SUNDHANI - Academia.edu (original) (raw)

Papers by ELZA SUNDHANI

Molecules

Co-administered medicinal herbs can modify a drug’s pharmacokinetics (PK), effectiveness, and tox... more Co-administered medicinal herbs can modify a drug’s pharmacokinetics (PK), effectiveness, and toxicity. Andrographis paniculata (Burm. f.) ethanolic extract (APE) and andrographolide (AND) (a potent CYP2C9 inducer/inhibitor) can alter the pharmacokinetic parameters of glipizide (GLZ). This study aimed to determine the potential pharmacokinetics of herb–drug interactions between GLZ and APE/AND in the plasma of normal and diabetic rats using the HPLC bioanalysis method. The glipizide bioanalytical method established with RP-HPLC/UV instrument was validated following the EMA guidelines. GLZ was administered alone and in combination with APE or AND to normal and diabetic rats. The GLZ pharmacokinetic parameters were estimated according to the correlation between concentration and sampling time using the PK solver program. A simple and rapid GLZ bioanalysis technique with a lower limit of quantitation of 25 ng/mL was developed and presented the following parameters: accuracy (error ≤ 15...

Journal of Herbmed Pharmacology

Introduction: Herb–drug interactions (HDIs) in pharmacokinetics and pharmacodynamics can occur wh... more Introduction: Herb–drug interactions (HDIs) in pharmacokinetics and pharmacodynamics can occur when natural compounds are used in combination with drugs. This study aimed to review the potential interaction of Andrographis paniculata (Burm. f.) extract (APE) and its primary compound andrographolide (AND) with several drugs exhibiting various pharmacological activities.Methods: In this systematic review, articles were collected from international databases such as PubMed, Science Direct, Springer Link, and Scopus until August 2021. The following keywords were used: Andrographis paniculata, andrographolide, HDI, drug interaction, pharmacokinetics, and pharmacology. This review was written in accordance with the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA), SYRCLE’s risk of bias (RoB) tool for animal intervention studies, and Cochrane RoB 2 tool to analyze the RoB for qualitative assessment.Results: Twelve articles were included in accor...

Indonesian Journal of Chemistry

The study aims to analyze the potential Herb-Drug Interactions (HDIs) of the chemical compound in... more The study aims to analyze the potential Herb-Drug Interactions (HDIs) of the chemical compound in Andrographis paniculate Burm. f. against Constitutive Androstane Receptor (CAR) and Pregnane X Receptor (PXR). The 1XVP and 1SKX obtained from the Protein Data Bank (PDB) were used as the targeted protein. The molecular docking analysis was done using the Molecular Operating Environment (MOE) and molecular dynamics simulation using Gromacs. The results of the docking analysis showed that 14-Deoxy-11,12-didehydroandrographolide had the strongest binding energy (1XVP-21.0998 Å) with the Arene-H binding type on Tyr326 and Andrographidine A had the strongest binding energy (1SKX-24.7363 Å) with the Arene-H binding type on Trp299. While Andrographolide is the major component, it also has a high affinity for the two PDB IDs (1XVP-17.4044 Å and 1SKX-21.8881 Å). Based on the RMSD value, the radius of gyration (Rg), and MM/PBSA on molecular dynamic simulations, it shows that the ligand and prote...

Polymer-Plastics Technology and Engineering, 2017

Journal of Pharmacy And Bioallied Sciences

Asian Journal of Pharmaceutical and Clinical Research

Objective: Garcinia mangostana is a plant that can be used as a traditional medicine to treat var... more Objective: Garcinia mangostana is a plant that can be used as a traditional medicine to treat various infectious diseases for the treatment of diarrhea, skin infection, and chronic wounds. The activity as antifungal and antibacterial of juice and ethanolic extract from G. mangostana leaves were investigated. Methods: Juice and ethanolic extract were concentrated using a rotary evaporator to get concentrated extract with rendemen 2.571 and 5.647% (w/w). Juice and ethanolic extract dilution method were employed to evaluate the antifungal activity against Saccharomyces cerevisiae. Ethanolic extract dilution method was used to assess the antibacterial activity against Bacillus subtilis and Escherichia coli. Results: The results of this research showed that juice and ethanolic extract were effective against S. cerevisiae, and the minimum inhibitory concentration was 1000, and 500 mg/mL. Antibacterial activity of the G. mangostana leaves ethanolic extract showed that the action was potent...

International Journal of Applied Pharmaceutics

Objective: The objective of this research was to screen phytochemical constituents and determine ... more Objective: The objective of this research was to screen phytochemical constituents and determine the activity of latex from Musa paradisiacavar. sapientum and Carica papaya L. to the process of wound healing in NIH3T3 fibroblasts cells through observations of the proliferation andmigration of cells.Materials and Methods: Screening phytochemical compounds of latex from M. paradisiaca var. sapientum and C. papaya L. used chemical reagent.Cytotoxic activity using 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) method. The proliferation test used the doubling timemethod at a susceptible incubation time of 0, 24, 48, and 72 h with a concentration of 25 μg/mL–250 μg/mL. The migration test was carried out usingthe scratch wound healing method with a concentration of 25 μg/mL–250 μg/mL in the time range of 0, 12, 24, and 48 h.Results: Phytochemical compounds contained in the latex from M. paradisiaca var. sapientum (saponin and tannin) and C. papaya L. (saponin andalkalo...

International Journal of Applied Pharmaceutics

Objective: Electrolyzed oxidizing/reducing water is popular as health beneficial water in Indones... more Objective: Electrolyzed oxidizing/reducing water is popular as health beneficial water in Indonesia. In this study, we examined the level of antibacterial,anticancer, and antioxidant activity of the electrolyzed water.Methods: The efficacy of electrolyzed water produced by Enagic® at six level pH (2.5, 6.0, 7.0, 8.5, 9.0, and 9.5) was investigated. Antibacterialactivity was evaluated using a macrodilution method. The anticancer activity was performed against human breast cancer (T47D) cell lines using3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Moreover, the antioxidant activity was determined using antioxidant model,2,2-diphenyl-1picrylhydrazyl radical scavenging activity.Results: The results show that electrolyzed water exhibited antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis.Among six level pH, electrolyzed water at pH 2.5 showed the highest antibacterial activity. The in vitro cytotoxic activity of electrolyzed water...

JURNAL ILMU KEFARMASIAN INDONESIA

Daun Kelor (Moringa oleifera) merupakan salah satu tanaman yang mengandung senyawa fenol seperti... more Daun Kelor (Moringa oleifera) merupakan salah satu tanaman yang mengandung senyawa fenol seperti alkaloid tannin saponin dan flavonoid yang berkhasiat sebagai antioksidan dan anti aging. Penelitian ini bertujuan untuk membuktikan aktivitas antioksidan dan anti aging daun kelor serta membuat Formulasi Body Butter dari ekstrak daun kelor. Proses ekstraksi daun kelor metode Maserasi dengan pelarut etanol 70 % perbandingan 1:10 kemudian dilakukan uji aktivitas antioksidan dengan metode beta caroten bleaching (BCB) dan anti aging selanjutnya dilakukan formulasi body butter dengan bahan aktif ekstrak daun kelor 0,5 %. Hasil penetapan kadar Fenolik total dan Flavonoid total ekstrak daun kelor menunjukkan kadar yang dihasilkan yaitu sebesar 10,45 mgGAE/g ekstrak untuk fenolik total dan 5,53 % untuk Flavonoid Total. Hasil penelitian menunjukkan bahwa ekstrak daun kelor memiliki aktivitas penghambatan proses pemucatan beta karoten (inhibitor BCB) dan inhibitor enzim kolagenase dengan persen...

Pharmaciana

5-Fluorouracil (5-FU) is chemotherapeutic agents used in breast cancer treatment. However 5-FU ha... more 5-Fluorouracil (5-FU) is chemotherapeutic agents used in breast cancer treatment. However 5-FU has low effectiveness as chemotherapeutic agent after longterm used. 5-FU usually combined with other compounds to increase its effectivity. Bawang Dayak has been widely used empirically as a cancer treatment, especially breast cancer. The aim of this study was to determine the sensitivity of 5-FU as cytotoxic agent against T47D breast cancer cells and their combination with extract of Bawang Dayak (EBD), to determine apoptotic induction mechanism, and the inhibitory effect of T47D breast cancer cell cycle. The cytotoxicity properties were determined using the MTT method with the concetration series of 5-FU (0-600 μg/mL) and EBD (0-250 μg/mL). The combination between 5-FU (2-250 μg/mL and EBD (5-50 μg/mL) was used for combination treatment on T47D cells. The apoptotic cells after treatment were determined by propidium iodide/annexin V reagent using flowcytometry. The modulation effect of combination treatment between 5-FU and EBD was performed by determination of DNA content using flowcytometry. The treatment of 5-FU and EBD inhibited T47D cell growth with IC 50 value 274 μg/mL and 75 μg/mL, respectively. The combination therapy of 5-FU and EBD resulted a mild to strong synergistic effect, with combination index (CI) 0.829 and 0.779. The combined treatment (125μg/mL 5-FU and 25μg/mL EBD) induced apoptosis in T47D cells by 21.33%. This combination caused 57.11% cell accumulation in G 0-G 1 phase, that greater than control grup (41.20%). The combination treatment (5-FU and EBD) cause cell distubance and damage that lead to G 0-G 1 phase cell cycle arrest. The cell cycle can not proceed to the next phase. EBD produce synergistic effect when used as breast cancer co-chemotherapy with 5-FU. It was particularly able to induce apoptosis against T47D cells followed by G 0-G 1 phase accumulation. The extract of Bawang Dayak has a high potential to be developed as co-chemotherapy in breast cancer.

Molecules

Co-administered medicinal herbs can modify a drug’s pharmacokinetics (PK), effectiveness, and tox... more Co-administered medicinal herbs can modify a drug’s pharmacokinetics (PK), effectiveness, and toxicity. Andrographis paniculata (Burm. f.) ethanolic extract (APE) and andrographolide (AND) (a potent CYP2C9 inducer/inhibitor) can alter the pharmacokinetic parameters of glipizide (GLZ). This study aimed to determine the potential pharmacokinetics of herb–drug interactions between GLZ and APE/AND in the plasma of normal and diabetic rats using the HPLC bioanalysis method. The glipizide bioanalytical method established with RP-HPLC/UV instrument was validated following the EMA guidelines. GLZ was administered alone and in combination with APE or AND to normal and diabetic rats. The GLZ pharmacokinetic parameters were estimated according to the correlation between concentration and sampling time using the PK solver program. A simple and rapid GLZ bioanalysis technique with a lower limit of quantitation of 25 ng/mL was developed and presented the following parameters: accuracy (error ≤ 15...

Journal of Herbmed Pharmacology

Introduction: Herb–drug interactions (HDIs) in pharmacokinetics and pharmacodynamics can occur wh... more Introduction: Herb–drug interactions (HDIs) in pharmacokinetics and pharmacodynamics can occur when natural compounds are used in combination with drugs. This study aimed to review the potential interaction of Andrographis paniculata (Burm. f.) extract (APE) and its primary compound andrographolide (AND) with several drugs exhibiting various pharmacological activities.Methods: In this systematic review, articles were collected from international databases such as PubMed, Science Direct, Springer Link, and Scopus until August 2021. The following keywords were used: Andrographis paniculata, andrographolide, HDI, drug interaction, pharmacokinetics, and pharmacology. This review was written in accordance with the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA), SYRCLE’s risk of bias (RoB) tool for animal intervention studies, and Cochrane RoB 2 tool to analyze the RoB for qualitative assessment.Results: Twelve articles were included in accor...

Indonesian Journal of Chemistry

The study aims to analyze the potential Herb-Drug Interactions (HDIs) of the chemical compound in... more The study aims to analyze the potential Herb-Drug Interactions (HDIs) of the chemical compound in Andrographis paniculate Burm. f. against Constitutive Androstane Receptor (CAR) and Pregnane X Receptor (PXR). The 1XVP and 1SKX obtained from the Protein Data Bank (PDB) were used as the targeted protein. The molecular docking analysis was done using the Molecular Operating Environment (MOE) and molecular dynamics simulation using Gromacs. The results of the docking analysis showed that 14-Deoxy-11,12-didehydroandrographolide had the strongest binding energy (1XVP-21.0998 Å) with the Arene-H binding type on Tyr326 and Andrographidine A had the strongest binding energy (1SKX-24.7363 Å) with the Arene-H binding type on Trp299. While Andrographolide is the major component, it also has a high affinity for the two PDB IDs (1XVP-17.4044 Å and 1SKX-21.8881 Å). Based on the RMSD value, the radius of gyration (Rg), and MM/PBSA on molecular dynamic simulations, it shows that the ligand and prote...

Polymer-Plastics Technology and Engineering, 2017

Journal of Pharmacy And Bioallied Sciences

Asian Journal of Pharmaceutical and Clinical Research

Objective: Garcinia mangostana is a plant that can be used as a traditional medicine to treat var... more Objective: Garcinia mangostana is a plant that can be used as a traditional medicine to treat various infectious diseases for the treatment of diarrhea, skin infection, and chronic wounds. The activity as antifungal and antibacterial of juice and ethanolic extract from G. mangostana leaves were investigated. Methods: Juice and ethanolic extract were concentrated using a rotary evaporator to get concentrated extract with rendemen 2.571 and 5.647% (w/w). Juice and ethanolic extract dilution method were employed to evaluate the antifungal activity against Saccharomyces cerevisiae. Ethanolic extract dilution method was used to assess the antibacterial activity against Bacillus subtilis and Escherichia coli. Results: The results of this research showed that juice and ethanolic extract were effective against S. cerevisiae, and the minimum inhibitory concentration was 1000, and 500 mg/mL. Antibacterial activity of the G. mangostana leaves ethanolic extract showed that the action was potent...

International Journal of Applied Pharmaceutics

Objective: The objective of this research was to screen phytochemical constituents and determine ... more Objective: The objective of this research was to screen phytochemical constituents and determine the activity of latex from Musa paradisiacavar. sapientum and Carica papaya L. to the process of wound healing in NIH3T3 fibroblasts cells through observations of the proliferation andmigration of cells.Materials and Methods: Screening phytochemical compounds of latex from M. paradisiaca var. sapientum and C. papaya L. used chemical reagent.Cytotoxic activity using 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) method. The proliferation test used the doubling timemethod at a susceptible incubation time of 0, 24, 48, and 72 h with a concentration of 25 μg/mL–250 μg/mL. The migration test was carried out usingthe scratch wound healing method with a concentration of 25 μg/mL–250 μg/mL in the time range of 0, 12, 24, and 48 h.Results: Phytochemical compounds contained in the latex from M. paradisiaca var. sapientum (saponin and tannin) and C. papaya L. (saponin andalkalo...

International Journal of Applied Pharmaceutics

Objective: Electrolyzed oxidizing/reducing water is popular as health beneficial water in Indones... more Objective: Electrolyzed oxidizing/reducing water is popular as health beneficial water in Indonesia. In this study, we examined the level of antibacterial,anticancer, and antioxidant activity of the electrolyzed water.Methods: The efficacy of electrolyzed water produced by Enagic® at six level pH (2.5, 6.0, 7.0, 8.5, 9.0, and 9.5) was investigated. Antibacterialactivity was evaluated using a macrodilution method. The anticancer activity was performed against human breast cancer (T47D) cell lines using3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Moreover, the antioxidant activity was determined using antioxidant model,2,2-diphenyl-1picrylhydrazyl radical scavenging activity.Results: The results show that electrolyzed water exhibited antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis.Among six level pH, electrolyzed water at pH 2.5 showed the highest antibacterial activity. The in vitro cytotoxic activity of electrolyzed water...

JURNAL ILMU KEFARMASIAN INDONESIA

Daun Kelor (Moringa oleifera) merupakan salah satu tanaman yang mengandung senyawa fenol seperti... more Daun Kelor (Moringa oleifera) merupakan salah satu tanaman yang mengandung senyawa fenol seperti alkaloid tannin saponin dan flavonoid yang berkhasiat sebagai antioksidan dan anti aging. Penelitian ini bertujuan untuk membuktikan aktivitas antioksidan dan anti aging daun kelor serta membuat Formulasi Body Butter dari ekstrak daun kelor. Proses ekstraksi daun kelor metode Maserasi dengan pelarut etanol 70 % perbandingan 1:10 kemudian dilakukan uji aktivitas antioksidan dengan metode beta caroten bleaching (BCB) dan anti aging selanjutnya dilakukan formulasi body butter dengan bahan aktif ekstrak daun kelor 0,5 %. Hasil penetapan kadar Fenolik total dan Flavonoid total ekstrak daun kelor menunjukkan kadar yang dihasilkan yaitu sebesar 10,45 mgGAE/g ekstrak untuk fenolik total dan 5,53 % untuk Flavonoid Total. Hasil penelitian menunjukkan bahwa ekstrak daun kelor memiliki aktivitas penghambatan proses pemucatan beta karoten (inhibitor BCB) dan inhibitor enzim kolagenase dengan persen...

Pharmaciana

5-Fluorouracil (5-FU) is chemotherapeutic agents used in breast cancer treatment. However 5-FU ha... more 5-Fluorouracil (5-FU) is chemotherapeutic agents used in breast cancer treatment. However 5-FU has low effectiveness as chemotherapeutic agent after longterm used. 5-FU usually combined with other compounds to increase its effectivity. Bawang Dayak has been widely used empirically as a cancer treatment, especially breast cancer. The aim of this study was to determine the sensitivity of 5-FU as cytotoxic agent against T47D breast cancer cells and their combination with extract of Bawang Dayak (EBD), to determine apoptotic induction mechanism, and the inhibitory effect of T47D breast cancer cell cycle. The cytotoxicity properties were determined using the MTT method with the concetration series of 5-FU (0-600 μg/mL) and EBD (0-250 μg/mL). The combination between 5-FU (2-250 μg/mL and EBD (5-50 μg/mL) was used for combination treatment on T47D cells. The apoptotic cells after treatment were determined by propidium iodide/annexin V reagent using flowcytometry. The modulation effect of combination treatment between 5-FU and EBD was performed by determination of DNA content using flowcytometry. The treatment of 5-FU and EBD inhibited T47D cell growth with IC 50 value 274 μg/mL and 75 μg/mL, respectively. The combination therapy of 5-FU and EBD resulted a mild to strong synergistic effect, with combination index (CI) 0.829 and 0.779. The combined treatment (125μg/mL 5-FU and 25μg/mL EBD) induced apoptosis in T47D cells by 21.33%. This combination caused 57.11% cell accumulation in G 0-G 1 phase, that greater than control grup (41.20%). The combination treatment (5-FU and EBD) cause cell distubance and damage that lead to G 0-G 1 phase cell cycle arrest. The cell cycle can not proceed to the next phase. EBD produce synergistic effect when used as breast cancer co-chemotherapy with 5-FU. It was particularly able to induce apoptosis against T47D cells followed by G 0-G 1 phase accumulation. The extract of Bawang Dayak has a high potential to be developed as co-chemotherapy in breast cancer.