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Papers by Edisson Tello Camacho
Marine Drugs, 2019
Soft corals (Cnidaria, Anthozoa, Octocorallia) are a diverse group of marine invertebrates that i... more Soft corals (Cnidaria, Anthozoa, Octocorallia) are a diverse group of marine invertebrates that inhabit various marine environments in tropical and subtropical areas. Several species are recognized as prolific sources of compounds with a wide array of biological activities. Recent advances in analytical techniques, supported by robust statistical analyses, have allowed the analysis and characterization of the metabolome present in a single living organism. In this study, a liquid chromatography-high resolution mass spectrometry metabolomic approach was applied to analyze the metabolite composition of 28 soft corals present in the Caribbean coast of Colombia. Multivariate data analysis was used to correlate the chemical fingerprints of soft corals with their cytotoxic activity against tumor cell lines for anticancer purpose. Some diterpenoids were identified as specific markers to discriminate between cytotoxic and non-cytotoxic crude extracts of soft corals against tumor cell lines....
BMC microbiology, Jan 8, 2017
Water evaporation in solar salterns creates salinity gradients that promote the adaptation of mic... more Water evaporation in solar salterns creates salinity gradients that promote the adaptation of microbial species to different salinities. This competitive habitat challenges the metabolic capabilities of microorganisms and promotes alterations in their production of secondary metabolites. Thus, solar salterns are a potentially important source of new natural products. In Colombia, the most important and representative solar saltern is located in Manaure (La Guajira) in the north of Colombia. The aim of this study was to develop an alternative screening strategy to select halophilic bacteria as producers of bioactive compounds from mixed microbial cultures rather than individual environmental isolates. Brine and sediment samples from different ponds (across a salinity gradient) were inoculated in seven different culture media to grow bacteria and archaea, allowing for a total of 40 different mixed cultures. An organic extract from each mixed culture was obtained and tested against mul...
Tetrahedron, 2011
Eight cembranoid epimers at C-8 (1e8) were isolated from the organic extract of the Colombian Car... more Eight cembranoid epimers at C-8 (1e8) were isolated from the organic extract of the Colombian Caribbean octocoral Pseudoplexaura flagellosa. Compounds 2, 4, and 6 are reported for the first time. Although compounds 1, 3, 5, 7, and 8 have been reported previously, their structures and NMR assignments are revised, completed or corrected. The structures of these compounds were established on the basis of detailed analysis of their spectroscopic data. Furthermore, the relative configurations of compounds 1e3 and 7 were confirmed by single-crystal X-ray diffraction. The absolute configurations of compounds 1e8 were determined using a combination of the modified Mosher method and unambiguous chemical interconversions. Evaluation of their antifouling properties using quorum sensing inhibition (QSI) and biofilm inhibition bioassays showed that compounds 3, 6, and 7 have excellent QSI activity against Chromobacterium violaceum, as measured by inhibition of the production of violacein pigment, without interfering with its growth. Furthermore, compounds 3, 5, 6, and 8 exhibited inhibition of biofilm maturation without interfering in the growth of Pseudomonas aeruginosa, Vibrio harveyi, and Staphylococcus aureus. This is the first report of cembranoids as inhibitors of bacterial biofilm and as compounds that interfere with QS in C. violaceum.
Deseo expresar mis agradecimientos: A la UNIVERSIDAD NACIONAL DE COLOMBIA por permitirme realizar... more Deseo expresar mis agradecimientos: A la UNIVERSIDAD NACIONAL DE COLOMBIA por permitirme realizar mis estudios a nivel de Doctorado. A COLCIENCIAS por la beca del programa de formación doctoral "Francisco José de Caldas"-2009 para el desarrollo de mi Doctorado y por la financiación parcial de esta investigación.
International Journal of Agricultural and Biological Engineering, 2018
Microalgae are widely used in the pharmaceutical and energy industries, therefore the conditions ... more Microalgae are widely used in the pharmaceutical and energy industries, therefore the conditions for their cultivation and extraction methods play an important role in the profiling and acquisition of lipids. The efficiency of lipid extraction from microalgae has attracted great interest from industry because of the wide variety of lipids and amounts that can be obtained. Acutodesmus obliquus (Scenedesmus obliquus UTEX 393) was used in this study. It was cultivated in Bold 3N medium modified with 75% nitrogen at 25°C, pH 6.8, 125 r/min and a photoperiod of 18/6 h and illuminated with white light provided by a Light-Emitting Diode Surface Mount Device extensions (LED SMD) with an intensity of 1200 µE/(m 2 • s). The cells were stained with the Red Nile (RN) technique to indicate lipid production. Four extraction methods were compared, classical, microwave (MW), Soxhlet, and ultrasound (US), using the same solvent proportions (hexane: chloroform: methanol=1 : 2 : 3). All samples were analyzed with Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography coupled to Mass Spectrometry (GC-MS). The results showed: 1) lipid production detected by RN was consistent with microalgal growth; 2) the MW technique was the best extraction method, according to the statistical analysis through Randomized Complete Block (RCB) design and performance of 4.6%; and 3) the presence of saturated and unsaturated acids was indicated by FTIR spectra. GC-MS was able to identify palmitic and linoleic acids as the likely major constituents of the sample.
Journal of Chemical Technology & Biotechnology, 2019
In-situ DRIFTS study of CO2 capture and methanation over Al2O3, 5% Ru/Al2O3, 6.1% "Na2O"... more In-situ DRIFTS study of CO2 capture and methanation over Al2O3, 5% Ru/Al2O3, 6.1% "Na2O"/Al2O3 sorbent phase and 5% Ru-6.1% "Na2O"/Al2O3 DFM. Each experiment was carried out using 35 mg of the sample and in-situ pre-reduce with 20% H2/N2 for 2 hours. The sheets are named according to samples name and data type (raw and processed). Final DRIFTS were obtain by substracting backgroud spectra of the sample at 320 °C under N2.
Marine Drugs, 2019
Soft corals (Cnidaria, Anthozoa, Octocorallia) are a diverse group of marine invertebrates that i... more Soft corals (Cnidaria, Anthozoa, Octocorallia) are a diverse group of marine invertebrates that inhabit various marine environments in tropical and subtropical areas. Several species are recognized as prolific sources of compounds with a wide array of biological activities. Recent advances in analytical techniques, supported by robust statistical analyses, have allowed the analysis and characterization of the metabolome present in a single living organism. In this study, a liquid chromatography-high resolution mass spectrometry metabolomic approach was applied to analyze the metabolite composition of 28 soft corals present in the Caribbean coast of Colombia. Multivariate data analysis was used to correlate the chemical fingerprints of soft corals with their cytotoxic activity against tumor cell lines for anticancer purpose. Some diterpenoids were identified as specific markers to discriminate between cytotoxic and non-cytotoxic crude extracts of soft corals against tumor cell lines....
BMC microbiology, Jan 8, 2017
Water evaporation in solar salterns creates salinity gradients that promote the adaptation of mic... more Water evaporation in solar salterns creates salinity gradients that promote the adaptation of microbial species to different salinities. This competitive habitat challenges the metabolic capabilities of microorganisms and promotes alterations in their production of secondary metabolites. Thus, solar salterns are a potentially important source of new natural products. In Colombia, the most important and representative solar saltern is located in Manaure (La Guajira) in the north of Colombia. The aim of this study was to develop an alternative screening strategy to select halophilic bacteria as producers of bioactive compounds from mixed microbial cultures rather than individual environmental isolates. Brine and sediment samples from different ponds (across a salinity gradient) were inoculated in seven different culture media to grow bacteria and archaea, allowing for a total of 40 different mixed cultures. An organic extract from each mixed culture was obtained and tested against mul...
Tetrahedron, 2011
Eight cembranoid epimers at C-8 (1e8) were isolated from the organic extract of the Colombian Car... more Eight cembranoid epimers at C-8 (1e8) were isolated from the organic extract of the Colombian Caribbean octocoral Pseudoplexaura flagellosa. Compounds 2, 4, and 6 are reported for the first time. Although compounds 1, 3, 5, 7, and 8 have been reported previously, their structures and NMR assignments are revised, completed or corrected. The structures of these compounds were established on the basis of detailed analysis of their spectroscopic data. Furthermore, the relative configurations of compounds 1e3 and 7 were confirmed by single-crystal X-ray diffraction. The absolute configurations of compounds 1e8 were determined using a combination of the modified Mosher method and unambiguous chemical interconversions. Evaluation of their antifouling properties using quorum sensing inhibition (QSI) and biofilm inhibition bioassays showed that compounds 3, 6, and 7 have excellent QSI activity against Chromobacterium violaceum, as measured by inhibition of the production of violacein pigment, without interfering with its growth. Furthermore, compounds 3, 5, 6, and 8 exhibited inhibition of biofilm maturation without interfering in the growth of Pseudomonas aeruginosa, Vibrio harveyi, and Staphylococcus aureus. This is the first report of cembranoids as inhibitors of bacterial biofilm and as compounds that interfere with QS in C. violaceum.
Deseo expresar mis agradecimientos: A la UNIVERSIDAD NACIONAL DE COLOMBIA por permitirme realizar... more Deseo expresar mis agradecimientos: A la UNIVERSIDAD NACIONAL DE COLOMBIA por permitirme realizar mis estudios a nivel de Doctorado. A COLCIENCIAS por la beca del programa de formación doctoral "Francisco José de Caldas"-2009 para el desarrollo de mi Doctorado y por la financiación parcial de esta investigación.
International Journal of Agricultural and Biological Engineering, 2018
Microalgae are widely used in the pharmaceutical and energy industries, therefore the conditions ... more Microalgae are widely used in the pharmaceutical and energy industries, therefore the conditions for their cultivation and extraction methods play an important role in the profiling and acquisition of lipids. The efficiency of lipid extraction from microalgae has attracted great interest from industry because of the wide variety of lipids and amounts that can be obtained. Acutodesmus obliquus (Scenedesmus obliquus UTEX 393) was used in this study. It was cultivated in Bold 3N medium modified with 75% nitrogen at 25°C, pH 6.8, 125 r/min and a photoperiod of 18/6 h and illuminated with white light provided by a Light-Emitting Diode Surface Mount Device extensions (LED SMD) with an intensity of 1200 µE/(m 2 • s). The cells were stained with the Red Nile (RN) technique to indicate lipid production. Four extraction methods were compared, classical, microwave (MW), Soxhlet, and ultrasound (US), using the same solvent proportions (hexane: chloroform: methanol=1 : 2 : 3). All samples were analyzed with Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography coupled to Mass Spectrometry (GC-MS). The results showed: 1) lipid production detected by RN was consistent with microalgal growth; 2) the MW technique was the best extraction method, according to the statistical analysis through Randomized Complete Block (RCB) design and performance of 4.6%; and 3) the presence of saturated and unsaturated acids was indicated by FTIR spectra. GC-MS was able to identify palmitic and linoleic acids as the likely major constituents of the sample.
Journal of Chemical Technology & Biotechnology, 2019
In-situ DRIFTS study of CO2 capture and methanation over Al2O3, 5% Ru/Al2O3, 6.1% "Na2O"... more In-situ DRIFTS study of CO2 capture and methanation over Al2O3, 5% Ru/Al2O3, 6.1% "Na2O"/Al2O3 sorbent phase and 5% Ru-6.1% "Na2O"/Al2O3 DFM. Each experiment was carried out using 35 mg of the sample and in-situ pre-reduce with 20% H2/N2 for 2 hours. The sheets are named according to samples name and data type (raw and processed). Final DRIFTS were obtain by substracting backgroud spectra of the sample at 320 °C under N2.