Eduardo Almansa - Academia.edu (original) (raw)
Papers by Eduardo Almansa
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2004
To establish the changes which occur during embryogenesis and early larvae development, eggs, yol... more To establish the changes which occur during embryogenesis and early larvae development, eggs, yolk-sac larvae (one day old larvae) and absorbed yolk-sac larvae (three day old larvae) of white sea bream were examined for lipid class and fatty acid composition. The development was characterized by a decrease in all lipid classes with the exception of phosphatidylserine (PS) and fatty free acids (FFA) which increased, and sphingomyelin (SM) which remained unchanged. The changes observed in lipid class content and the decrease in fatty acids in total lipid (TL) reflect the utilization and mobilization of lipids during both embryogenesis and early larvae development. Fluctuations in the relative composition of fatty acids in phosphatidylcholine (PC) during development suggest a selective bulk uptake and catabolism of fatty acids in this lipid class. Unlike PC, catabolism of triacylglycerol (TG) fatty acid appears to be non-selective. During development, the decrease in levels of polyunsaturated fatty acids (PUFA) eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) in total lipid denotes their utilization as energy substrate by Diplodus sargus larvae.
To establish the changes which occur during embryogenesis and early larvae development, eggs, yol... more To establish the changes which occur during embryogenesis and early larvae development, eggs, yolk-sac larvae (one day old larvae) and absorbed yolk-sac larvae (three day old larvae) of white sea bream were examined for lipid class and fatty acid composition. The development was characterized by a decrease in all lipid classes with the exception of phosphatidylserine (PS) and fatty free acids (FFA) which increased, and sphingomyelin (SM) which remained unchanged. The changes observed in lipid class content and the decrease in fatty acids in total lipid (TL) reflect the utilization and mobilization of lipids during both embryogenesis and early larvae development. Fluctuations in the relative composition of fatty acids in phosphatidylcholine (PC) during development suggest a selective bulk uptake and catabolism of fatty acids in this lipid class. Unlike PC, catabolism of triacylglycerol (TG) fatty acid appears to be non-selective. During development, the decrease in levels of polyunsaturated fatty acids (PUFA) eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) in total lipid denotes their utilization as energy substrate by Diplodus sargus larvae.
ABSTRACT Brill (Scophthalmus rhombus L.) is a flatfish considered of special interest for aquacul... more ABSTRACT Brill (Scophthalmus rhombus L.) is a flatfish considered of special interest for aquaculture diversification, but the high mortality observed during the early larval rearing is the main obstacle to commercial culture. The objective of this study was to contribute to the knowledge of nutrient utilization of early‐hatched larvae, characterizing the changes in lipid and protein contents during embryogenesis and the yolk‐sac larval stage of S. rhombus. Total lipid, lipid classes and fatty acid contents remained constant during embryogenesis and yolk‐sac larval development, except for phosphatidylethanolamine and phosphatidylinositol, which increased in quantity during the yolk‐sac larval stage. On the other hand, total protein (including non‐protein nitrogen) and amino acids decreased their contents in both periods, especially at hatching. The decrease only in the serine, glutamic acid, proline and lysine contents during embryogenesis suggests a selective use of amino acids during this phase. Unlike embryogenesis, amino acids loss during hatching appears to be non‐selective, and almost all amino acids (essential and non‐essential) decreased. Our results suggest that there is higher catabolism of protein vs. lipid during embryogenesis and the yolk‐sac larval stage of S. rhombus.
Journal of Neuroscience Methods, 2006
Most studies aimed at exploring the molecular and cellular properties of plasma membranes in neur... more Most studies aimed at exploring the molecular and cellular properties of plasma membranes in neural tissues make use of cell lines. However, cell membrane lipid composition of cell lines is notably different from that of brain tissues where they presumably derive from. Using septal-derived SN56 cells and hippocampal-derived HT22 cells, we demonstrated that cell lines exhibit lower contents of saturated
Communications in agricultural and applied biological sciences
Artemia sp. is widely used in fish culture and has been used in octopus paralarval culture. Howev... more Artemia sp. is widely used in fish culture and has been used in octopus paralarval culture. However, it lacks n-3 long-chain polyunsaturated fatty acids (LC-PUFA), which are essential for marine organisms. Therefore, lipid enrichment is performed to improve Artemia lipid profile. This study determined the “in vivo” capability of Artemia nauplii to metabolize 14C-labelled 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA), and 22:6n-3 (DHA). Total incorporation of C18 fatty acids was similar, with esterification into triacylglycerols, and phosphatidylcholine (PC) and phosphatidylethanolamine (PE). In contrast, incorporation of LC-PUFA varied with fatty acid, with DHA presenting the lowest and ARA the highest values. EPA and ARA were primarily incorporated into PC, PE, phosphatidylinositol and phosphatidylserine. Further studies are being conducted to elucidate the esterification of DHA into the different lipid classes.
This in vivo study shows that esterification pattern of radiolabeled ARA, EPA and DHA, into the d... more This in vivo study shows that esterification pattern of radiolabeled ARA, EPA and DHA, into the different lipid classes of Octopus vulgaris hatchlings and Artemia sp. nauplii, is highly different, with a greater specificity in octopus for esterification of DHA into phosphatidylcholine and EPA and ARA into phosphatidylethanolamine. This fact should be taken into to account in octopus nutrition since it may influence rearing success, particularly if live prey metabolism commits availability of essential nutrients.
Communications in agricultural and applied biological sciences
Journal of Fish Biology, 2001
In female sea bream Sparus aurata fed a control diet (C), ovarian levels of neutral lipids (NL) a... more In female sea bream Sparus aurata fed a control diet (C), ovarian levels of neutral lipids (NL) and polar lipids (PL) remained constant between November and March, while a decrease in NL content was observed in liver and muscle. In the same period, liver PL content increased, while no changes were observed in muscle. Between March and June ovarian NL and PL showed a strong decrease, while NL remained constant in liver and muscle. When fish were fed a diet lacking in n-3 highly unsaturated fatty acids, n-3 HUFA (D), the pattern observed was similar to that found in the fish fed diet C, with the exception of liver NL, which increased between March and June. In general, the changes in fatty acid content, in both groups of fish, were highly influenced by the diet given to the broodstock, although these effects were greater on ovarian NL and PL than on liver and muscle lipids. Despite the fact that gilthead seabream females continue feeding during the spawning season, they probably make use of their liver and muscle reserves during the gonadal maturation process. Furthermore, the fatty acid composition of the broodstock diet was reflected in the body composition, especially in the ovaries.
Journal of Comparative Physiology B-biochemical Systemic and Environmental Physiology, 2003
The temperature dependence for Na+-K+-ATPase has been examined in the proximal-distal axis of the... more The temperature dependence for Na+-K+-ATPase has been examined in the proximal-distal axis of the intestine of gilthead seabream (Sparus aurata), i.e. pyloric caeca (PC), anterior intestine (AI) and posterior intestine (PI). Data derived from the Arrhenius plots showed differences in terms of temperature discontinuity points (Td) (13.29 °C, 16.39 °C and 17.48 °C for PC, AI and PI, respectively) and
Journal of Comparative Physiology B-biochemical Systemic and Environmental Physiology, 2001
The activity of the Na+-K+-ATPase along the intestinal mucosa of the gilthead seabream has been e... more The activity of the Na+-K+-ATPase along the intestinal mucosa of the gilthead seabream has been examined. Under optimal assay conditions, found at 35 °C, pH 7.5, 2-5 mM MgCl2, 5 mM ATP, 10 mM K+ and 200 mM Na+, maximal Na+-K+-ATPase activities were found in the microsomal fraction of pyloric caeca (PC) and anterior intestine (AI), which were more than
Journal of Shellfish Research, 2014
Aquaculture Nutrition, 2009
The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Pro... more The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Procambarus clarkii) and fish (Sardina pilchardus) and two semi-humid artificial diets (based on fish or shrimp powder) to the cuttlefish, Sepia officinalis, were analysed. Growth rate and feeding rate [FR; % body weight (BW) day−1] and food conversions (FC, %) were determined. Cuttlefish fed shrimp grew larger (3.8% BW day−1) and had the highest FC, followed by those fed crayfish, and sardine. The highest FR was obtained for cuttlefish fed crayfish (10.5% BW day−1). Although both artificial diets were accepted, none produced growth. Digestive gland-to-body weight ratio (DG/BW ratio) was calculated for animals fed each diet. A positive correlation (r = 0.94) between cuttlefish ingestion FR and DG weight was obtained. Mortality occurred mainly during the last week, and some cannibalism occurred among cuttlefish fed artificial diets. Finally, lipid composition of diets, DG and mantle of each group were analysed. Sardine diet was characterized by high levels of triacylglycerol (TG), whereas the main difference between shrimp and crayfish was the higher n-3/n-6 ratio found in shrimp. Changes in the lipid composition of DG were related to diet, but did not correlate with growth data. A strong loss of TG in the DG of artificial diets groups was notable. No differences in mantle lipid composition among the natural diets were found, but artificial diet groups showed higher contents of neutral lipids in their mantle respect to natural diets. According to results obtained, crayfish (P. clarkii) could be used as an alternative prey for rearing S. officinalis compared with shrimp. Artificial diets showed the worst effects in growth and mortality as well as the stronger influence on DG and mantle lipid composition of cuttlefish.
Journal of Molluscan Studies, 2007
Journal of Biotechnology, 2010
Helgoland Marine Research, 2013
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2004
To establish the changes which occur during embryogenesis and early larvae development, eggs, yol... more To establish the changes which occur during embryogenesis and early larvae development, eggs, yolk-sac larvae (one day old larvae) and absorbed yolk-sac larvae (three day old larvae) of white sea bream were examined for lipid class and fatty acid composition. The development was characterized by a decrease in all lipid classes with the exception of phosphatidylserine (PS) and fatty free acids (FFA) which increased, and sphingomyelin (SM) which remained unchanged. The changes observed in lipid class content and the decrease in fatty acids in total lipid (TL) reflect the utilization and mobilization of lipids during both embryogenesis and early larvae development. Fluctuations in the relative composition of fatty acids in phosphatidylcholine (PC) during development suggest a selective bulk uptake and catabolism of fatty acids in this lipid class. Unlike PC, catabolism of triacylglycerol (TG) fatty acid appears to be non-selective. During development, the decrease in levels of polyunsaturated fatty acids (PUFA) eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) in total lipid denotes their utilization as energy substrate by Diplodus sargus larvae.
To establish the changes which occur during embryogenesis and early larvae development, eggs, yol... more To establish the changes which occur during embryogenesis and early larvae development, eggs, yolk-sac larvae (one day old larvae) and absorbed yolk-sac larvae (three day old larvae) of white sea bream were examined for lipid class and fatty acid composition. The development was characterized by a decrease in all lipid classes with the exception of phosphatidylserine (PS) and fatty free acids (FFA) which increased, and sphingomyelin (SM) which remained unchanged. The changes observed in lipid class content and the decrease in fatty acids in total lipid (TL) reflect the utilization and mobilization of lipids during both embryogenesis and early larvae development. Fluctuations in the relative composition of fatty acids in phosphatidylcholine (PC) during development suggest a selective bulk uptake and catabolism of fatty acids in this lipid class. Unlike PC, catabolism of triacylglycerol (TG) fatty acid appears to be non-selective. During development, the decrease in levels of polyunsaturated fatty acids (PUFA) eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) in total lipid denotes their utilization as energy substrate by Diplodus sargus larvae.
ABSTRACT Brill (Scophthalmus rhombus L.) is a flatfish considered of special interest for aquacul... more ABSTRACT Brill (Scophthalmus rhombus L.) is a flatfish considered of special interest for aquaculture diversification, but the high mortality observed during the early larval rearing is the main obstacle to commercial culture. The objective of this study was to contribute to the knowledge of nutrient utilization of early‐hatched larvae, characterizing the changes in lipid and protein contents during embryogenesis and the yolk‐sac larval stage of S. rhombus. Total lipid, lipid classes and fatty acid contents remained constant during embryogenesis and yolk‐sac larval development, except for phosphatidylethanolamine and phosphatidylinositol, which increased in quantity during the yolk‐sac larval stage. On the other hand, total protein (including non‐protein nitrogen) and amino acids decreased their contents in both periods, especially at hatching. The decrease only in the serine, glutamic acid, proline and lysine contents during embryogenesis suggests a selective use of amino acids during this phase. Unlike embryogenesis, amino acids loss during hatching appears to be non‐selective, and almost all amino acids (essential and non‐essential) decreased. Our results suggest that there is higher catabolism of protein vs. lipid during embryogenesis and the yolk‐sac larval stage of S. rhombus.
Journal of Neuroscience Methods, 2006
Most studies aimed at exploring the molecular and cellular properties of plasma membranes in neur... more Most studies aimed at exploring the molecular and cellular properties of plasma membranes in neural tissues make use of cell lines. However, cell membrane lipid composition of cell lines is notably different from that of brain tissues where they presumably derive from. Using septal-derived SN56 cells and hippocampal-derived HT22 cells, we demonstrated that cell lines exhibit lower contents of saturated
Communications in agricultural and applied biological sciences
Artemia sp. is widely used in fish culture and has been used in octopus paralarval culture. Howev... more Artemia sp. is widely used in fish culture and has been used in octopus paralarval culture. However, it lacks n-3 long-chain polyunsaturated fatty acids (LC-PUFA), which are essential for marine organisms. Therefore, lipid enrichment is performed to improve Artemia lipid profile. This study determined the “in vivo” capability of Artemia nauplii to metabolize 14C-labelled 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA), and 22:6n-3 (DHA). Total incorporation of C18 fatty acids was similar, with esterification into triacylglycerols, and phosphatidylcholine (PC) and phosphatidylethanolamine (PE). In contrast, incorporation of LC-PUFA varied with fatty acid, with DHA presenting the lowest and ARA the highest values. EPA and ARA were primarily incorporated into PC, PE, phosphatidylinositol and phosphatidylserine. Further studies are being conducted to elucidate the esterification of DHA into the different lipid classes.
This in vivo study shows that esterification pattern of radiolabeled ARA, EPA and DHA, into the d... more This in vivo study shows that esterification pattern of radiolabeled ARA, EPA and DHA, into the different lipid classes of Octopus vulgaris hatchlings and Artemia sp. nauplii, is highly different, with a greater specificity in octopus for esterification of DHA into phosphatidylcholine and EPA and ARA into phosphatidylethanolamine. This fact should be taken into to account in octopus nutrition since it may influence rearing success, particularly if live prey metabolism commits availability of essential nutrients.
Communications in agricultural and applied biological sciences
Journal of Fish Biology, 2001
In female sea bream Sparus aurata fed a control diet (C), ovarian levels of neutral lipids (NL) a... more In female sea bream Sparus aurata fed a control diet (C), ovarian levels of neutral lipids (NL) and polar lipids (PL) remained constant between November and March, while a decrease in NL content was observed in liver and muscle. In the same period, liver PL content increased, while no changes were observed in muscle. Between March and June ovarian NL and PL showed a strong decrease, while NL remained constant in liver and muscle. When fish were fed a diet lacking in n-3 highly unsaturated fatty acids, n-3 HUFA (D), the pattern observed was similar to that found in the fish fed diet C, with the exception of liver NL, which increased between March and June. In general, the changes in fatty acid content, in both groups of fish, were highly influenced by the diet given to the broodstock, although these effects were greater on ovarian NL and PL than on liver and muscle lipids. Despite the fact that gilthead seabream females continue feeding during the spawning season, they probably make use of their liver and muscle reserves during the gonadal maturation process. Furthermore, the fatty acid composition of the broodstock diet was reflected in the body composition, especially in the ovaries.
Journal of Comparative Physiology B-biochemical Systemic and Environmental Physiology, 2003
The temperature dependence for Na+-K+-ATPase has been examined in the proximal-distal axis of the... more The temperature dependence for Na+-K+-ATPase has been examined in the proximal-distal axis of the intestine of gilthead seabream (Sparus aurata), i.e. pyloric caeca (PC), anterior intestine (AI) and posterior intestine (PI). Data derived from the Arrhenius plots showed differences in terms of temperature discontinuity points (Td) (13.29 °C, 16.39 °C and 17.48 °C for PC, AI and PI, respectively) and
Journal of Comparative Physiology B-biochemical Systemic and Environmental Physiology, 2001
The activity of the Na+-K+-ATPase along the intestinal mucosa of the gilthead seabream has been e... more The activity of the Na+-K+-ATPase along the intestinal mucosa of the gilthead seabream has been examined. Under optimal assay conditions, found at 35 °C, pH 7.5, 2-5 mM MgCl2, 5 mM ATP, 10 mM K+ and 200 mM Na+, maximal Na+-K+-ATPase activities were found in the microsomal fraction of pyloric caeca (PC) and anterior intestine (AI), which were more than
Journal of Shellfish Research, 2014
Aquaculture Nutrition, 2009
The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Pro... more The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Procambarus clarkii) and fish (Sardina pilchardus) and two semi-humid artificial diets (based on fish or shrimp powder) to the cuttlefish, Sepia officinalis, were analysed. Growth rate and feeding rate [FR; % body weight (BW) day−1] and food conversions (FC, %) were determined. Cuttlefish fed shrimp grew larger (3.8% BW day−1) and had the highest FC, followed by those fed crayfish, and sardine. The highest FR was obtained for cuttlefish fed crayfish (10.5% BW day−1). Although both artificial diets were accepted, none produced growth. Digestive gland-to-body weight ratio (DG/BW ratio) was calculated for animals fed each diet. A positive correlation (r = 0.94) between cuttlefish ingestion FR and DG weight was obtained. Mortality occurred mainly during the last week, and some cannibalism occurred among cuttlefish fed artificial diets. Finally, lipid composition of diets, DG and mantle of each group were analysed. Sardine diet was characterized by high levels of triacylglycerol (TG), whereas the main difference between shrimp and crayfish was the higher n-3/n-6 ratio found in shrimp. Changes in the lipid composition of DG were related to diet, but did not correlate with growth data. A strong loss of TG in the DG of artificial diets groups was notable. No differences in mantle lipid composition among the natural diets were found, but artificial diet groups showed higher contents of neutral lipids in their mantle respect to natural diets. According to results obtained, crayfish (P. clarkii) could be used as an alternative prey for rearing S. officinalis compared with shrimp. Artificial diets showed the worst effects in growth and mortality as well as the stronger influence on DG and mantle lipid composition of cuttlefish.
Journal of Molluscan Studies, 2007
Journal of Biotechnology, 2010
Helgoland Marine Research, 2013