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Papers by Eduardo Oyama Lins Fonseca

Research paper thumbnail of Phenotypic and genetic variation of Triatoma costalimai (Hemiptera: Reduviidae)

Revista da Sociedade Brasileira de Medicina Tropical

Introduction: We aimed to study intraspecific variation in Triatoma costalimai, a potential vecto... more Introduction: We aimed to study intraspecific variation in Triatoma costalimai, a potential vector of Chagas disease present in Brazil and Bolivia. Methods: We analyzed phenotypic (connexivum color patterns, wing morphometrics) and genetic variation (16S mtDNA) of three Brazilian T. costalimai populations. We compared 16S sequences with those of putative Bolivian T. costalimai and its sister species, T. jatai. Results: Brazilian populations had different connexivum color patterns and forewing shapes. A 16S mtDNA haplotype network showed a clear separation of Brazilian T. costalimai from both T. jatai and Bolivian T. costalimai. Conclusions: We report considerable variability in T. costalimai populations.

Research paper thumbnail of Triatomine fauna in the state of Bahia, Brazil: What changed after 40 years of the vector-control program?

Revista da Sociedade Brasileira de Medicina Tropical

MGR supervised students involved in field and laboratory research. CMMC, RFA, GMC, OMFS, CGSS, RF... more MGR supervised students involved in field and laboratory research. CMMC, RFA, GMC, OMFS, CGSS, RFS obtain the databases, GR-Jr curated the dataset and analyzed the data. GR-Jr, RG-G, and MGR drafted the first version of the manuscript. All authors contributed to the interpretation of results, read and commented on manuscript drafts, and approved the final version. Conflict of Interest: All authors disclose no conflicts of interest during the development of this study. We certify that there is no conflict of interest with any relationships or support. Financial Support: Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).

Research paper thumbnail of Aspectos Ecoepidemiológicos Da Leishmaniose Tegumentar Americana No Município De Ituberá, Baixo Sul Da Bahia

Revista Baiana de Saúde Pública, 1970

O município de Ituberá foi selecionado por compor área endêmica deLeishmaniose Tegumentar America... more O município de Ituberá foi selecionado por compor área endêmica deLeishmaniose Tegumentar Americana (LTA) no Baixo Sul da Bahia. O presente trabalhotem como objetivo discutir os aspectos ecoepidemiológicos da LTA neste município. Para aanálise do perfil epidemiológico foi consultada a base de dados do Sistema de Informaçãode Agravos de Notificação. O Inquérito Entomológico foi realizado entre 17.10 e 13.11 de2010, como preconizado pelo Ministério da Saúde. Foram capturados 11.383 exemplares,sendo identificadas 19 espécies. Acreditamos que o predomínio das espécies sinantrópicasNyssomyia whitmani e Nyssomyia intermedia decorre da interferência do homem no ambienterural de Ituberá. Sugerimos que essas sejam as principais vetoras da LTA à populaçãolocal, considerando ainda a alta antropofilia e a ocorrência em todos os pontos de coleta.Destacamos a elevada transmissão silvestre desta enfermidade, devido à exposição diáriados trabalhadores rurais ao vetor no interior da mata. A transmis...

Research paper thumbnail of Descrição De Focos Residuais De Triatoma Infestans (Klug, 1834) No Município De Novo Horizonte, Bahia

Revista Baiana de Saúde Pública, 1970

Triatoma infestans foi considerado, por anos, o principal vetor da doença deChagas no Brasil. A r... more Triatoma infestans foi considerado, por anos, o principal vetor da doença deChagas no Brasil. A redução dos índices de infestação predial associados à espécie conferiuao país, em 2006, certificação de eliminação da transmissão da doença por esse vetor.Porém, focos residuais foram encontrados no município de Novo Horizonte (BA), Brasil, apartir do ano de 2010. Diante desse panorama, foi desenvolvido um estudo com objetivode descrever os focos residuais de T. infestans no município de Novo Horizonte, área decaatinga da Bahia. As pesquisas foram realizadas na localidade de Fazenda Queimadas6ª, zona rural do município, nos anos de 2011 a 2013. Os triatomíneos coletados foramidentificados, triados e dissecados para diagnóstico molecular da infecção natural peloTrypanosoma cruzi e avaliação de suas fontes alimentares. Durante o estudo, foramcoletados 502 exemplares, distribuídos em dois focos residuais: em 2011 com 71 indivíduosno intra e peridomicílio; e, em 2013, com 431, apenas no peri...

Research paper thumbnail of Vigilância molecular de triatomíneos sinantrópicos na Bahia

Autores Gilmar José da Silva Ribeiro Júnior 1,3, Renato Reis Renato Reis , Carlos G. S. dos Santo... more Autores Gilmar José da Silva Ribeiro Júnior 1,3, Renato Reis Renato Reis , Carlos G. S. dos Santos Carlos G. S. dos Santos 6, Eduardo O. L. Fonseca Eduardo O. L. Fonseca 6, Roberto Fonseca Roberto Fonseca 6, Fernanda C. Lanza Fernanda C. Lanza 1, Rodrigo Gurgel-Gonçalves Rodrigo Gurgel-Gonçalves 2, Mitermayer G. dos Reis Mitermayer G. dos Reis 1,5,7, DIEGO LOPES PAIM MIRANDA 1,5 Instituição 1 CPqGM Fiocruz/BA Centro de Pesquisas Gonçalo Moniz, Fiocruz – BA (Rua Waldemar Falcão, 121, Candeal Salvador/BA.), 2 UNB Universidade de Brasília – Laboratório de Parasitologia Médica e Biologia de Vetores (Campus Universitário Darcy Ribeiro, Brasília-DF.), 3 FRB-DeVry Faculdade Ruy Barbosa DeVry. (Rua Theodomiro Baptista, 422 Rio Vermelho, Salvador BA.), 4 UNIFACS Universidade Salvador (Rua Doutor José Peroba, no 251, Edf. Civil Empresarial, Sobreloja – STIEP, Salvador BA), 5 UFBA Universidade Federal da Bahia (Rua Augusto Viana, s/n Palácio da Reitoria, Canela, Salvador, BA.), 6 SESAB (DIVEP-...

Research paper thumbnail of Susceptibility characterization of residual Brazilian populations of Triatoma infestans Klug, 1834 (Hemiptera: Reduviidae) to deltamethrin pyrethroid

Revista da Sociedade Brasileira de Medicina Tropical, 2015

Introduction: Despite years of efforts towards the elimination of Triatoma infestans in Brazil, r... more Introduction: Despite years of efforts towards the elimination of Triatoma infestans in Brazil, residual foci still persist in some areas of the States of Bahia and Rio Grande do Sul. The persistence of these T. infestans populations in the country has two different origins of equal concern: operational failures or insecticide resistance. Thus, the objective of this study was to characterize the susceptibility profi le of the residual Brazilian populations of T. infestans to deltamethrin. Methods: The susceptibility reference lineage was derived from Cipein/Argentina. The populations studied were manually collected using a dislodging agent in peridomiciles in the States of Bahia (Novo Horizonte) and of Rio Grande do Sul (Santa Rosa and Doutor Maurício Cardoso). Serial dilutions of deltamethrin were prepared and applied at the dorsal abdomen of fi rst instar nymphs. The control group received only pure acetone. Mortality was evaluated after 72h. Qualitative tests assessed the mortality of a diagnostic dose of 1xLD 99 (2.76ng a.i./nymph) determined for the susceptibility reference lineage. Results: The susceptibility profi le characterization of the T. infestans populations revealed an RR 50 ranging from 1.73 to 3.26. The mortality percentage in response to a diagnostic dose was 100%. The results obtained in the quantitative and qualitative assays corresponded for all populations. Conclusions: The results of this study indicate that the persistence of residual foci of T. infestans in Bahia and Rio Grande do Sul is not related to insecticide resistance but may be associated with operational failures. In Rio Grande do Sul, we must consider the possibility of continuous reinfestation by Argentinian individuals, which justifi es active and effi cient epidemiological surveillance.

Research paper thumbnail of Prevalência e fatores associados às geo-helmintíases em crianças residentes em municípios com baixo IDH no Norte e Nordeste brasileiros

Cadernos De Saude Publica, 2010

Stool surveys were conducted to estimate the prevalence and identify risk factors for geohelminth... more Stool surveys were conducted to estimate the prevalence and identify risk factors for geohelminth infections among children in ten Brazilian municipalities with low human development indices (HDI). Socioeconomic and environmental data were obtained from the children's parents or guardians, and stool samples were examined. The proportion of geohelminth infections according to target variables was calculated. Risk factors were evaluated using multilevel logistic regression. Of the 2,523 children, 36.5% were infected with one or more geohelminths (Ascaris lumbricoides, 25.1%; hookworm, 15.3%; Trichuris trichiura, 12.2%). Overall prevalence of geohelminth infections was 45.7% in rural areas and 32.2% in urban areas. Low family income (OR = 1.75; 1.38-2.23), low maternal schooling (OR = 1.69; 1.39-2.06), presence of garbage near the home (OR = 1.50; 1.22-1.84), and number of individuals in the household (OR = 1.41; 1.17-1.71) were associated with infection. In conclusion, geohelminth infections were closely related to socioeconomic conditions, thus emphasizing the importance of targeted public interventions to improve living conditions as part of sustainable prevention.

Research paper thumbnail of Phenotypic and genetic variation of Triatoma costalimai (Hemiptera: Reduviidae)

Revista da Sociedade Brasileira de Medicina Tropical

Introduction: We aimed to study intraspecific variation in Triatoma costalimai, a potential vecto... more Introduction: We aimed to study intraspecific variation in Triatoma costalimai, a potential vector of Chagas disease present in Brazil and Bolivia. Methods: We analyzed phenotypic (connexivum color patterns, wing morphometrics) and genetic variation (16S mtDNA) of three Brazilian T. costalimai populations. We compared 16S sequences with those of putative Bolivian T. costalimai and its sister species, T. jatai. Results: Brazilian populations had different connexivum color patterns and forewing shapes. A 16S mtDNA haplotype network showed a clear separation of Brazilian T. costalimai from both T. jatai and Bolivian T. costalimai. Conclusions: We report considerable variability in T. costalimai populations.

Research paper thumbnail of Triatomine fauna in the state of Bahia, Brazil: What changed after 40 years of the vector-control program?

Revista da Sociedade Brasileira de Medicina Tropical

MGR supervised students involved in field and laboratory research. CMMC, RFA, GMC, OMFS, CGSS, RF... more MGR supervised students involved in field and laboratory research. CMMC, RFA, GMC, OMFS, CGSS, RFS obtain the databases, GR-Jr curated the dataset and analyzed the data. GR-Jr, RG-G, and MGR drafted the first version of the manuscript. All authors contributed to the interpretation of results, read and commented on manuscript drafts, and approved the final version. Conflict of Interest: All authors disclose no conflicts of interest during the development of this study. We certify that there is no conflict of interest with any relationships or support. Financial Support: Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).

Research paper thumbnail of Aspectos Ecoepidemiológicos Da Leishmaniose Tegumentar Americana No Município De Ituberá, Baixo Sul Da Bahia

Revista Baiana de Saúde Pública, 1970

O município de Ituberá foi selecionado por compor área endêmica deLeishmaniose Tegumentar America... more O município de Ituberá foi selecionado por compor área endêmica deLeishmaniose Tegumentar Americana (LTA) no Baixo Sul da Bahia. O presente trabalhotem como objetivo discutir os aspectos ecoepidemiológicos da LTA neste município. Para aanálise do perfil epidemiológico foi consultada a base de dados do Sistema de Informaçãode Agravos de Notificação. O Inquérito Entomológico foi realizado entre 17.10 e 13.11 de2010, como preconizado pelo Ministério da Saúde. Foram capturados 11.383 exemplares,sendo identificadas 19 espécies. Acreditamos que o predomínio das espécies sinantrópicasNyssomyia whitmani e Nyssomyia intermedia decorre da interferência do homem no ambienterural de Ituberá. Sugerimos que essas sejam as principais vetoras da LTA à populaçãolocal, considerando ainda a alta antropofilia e a ocorrência em todos os pontos de coleta.Destacamos a elevada transmissão silvestre desta enfermidade, devido à exposição diáriados trabalhadores rurais ao vetor no interior da mata. A transmis...

Research paper thumbnail of Descrição De Focos Residuais De Triatoma Infestans (Klug, 1834) No Município De Novo Horizonte, Bahia

Revista Baiana de Saúde Pública, 1970

Triatoma infestans foi considerado, por anos, o principal vetor da doença deChagas no Brasil. A r... more Triatoma infestans foi considerado, por anos, o principal vetor da doença deChagas no Brasil. A redução dos índices de infestação predial associados à espécie conferiuao país, em 2006, certificação de eliminação da transmissão da doença por esse vetor.Porém, focos residuais foram encontrados no município de Novo Horizonte (BA), Brasil, apartir do ano de 2010. Diante desse panorama, foi desenvolvido um estudo com objetivode descrever os focos residuais de T. infestans no município de Novo Horizonte, área decaatinga da Bahia. As pesquisas foram realizadas na localidade de Fazenda Queimadas6ª, zona rural do município, nos anos de 2011 a 2013. Os triatomíneos coletados foramidentificados, triados e dissecados para diagnóstico molecular da infecção natural peloTrypanosoma cruzi e avaliação de suas fontes alimentares. Durante o estudo, foramcoletados 502 exemplares, distribuídos em dois focos residuais: em 2011 com 71 indivíduosno intra e peridomicílio; e, em 2013, com 431, apenas no peri...

Research paper thumbnail of Vigilância molecular de triatomíneos sinantrópicos na Bahia

Autores Gilmar José da Silva Ribeiro Júnior 1,3, Renato Reis Renato Reis , Carlos G. S. dos Santo... more Autores Gilmar José da Silva Ribeiro Júnior 1,3, Renato Reis Renato Reis , Carlos G. S. dos Santos Carlos G. S. dos Santos 6, Eduardo O. L. Fonseca Eduardo O. L. Fonseca 6, Roberto Fonseca Roberto Fonseca 6, Fernanda C. Lanza Fernanda C. Lanza 1, Rodrigo Gurgel-Gonçalves Rodrigo Gurgel-Gonçalves 2, Mitermayer G. dos Reis Mitermayer G. dos Reis 1,5,7, DIEGO LOPES PAIM MIRANDA 1,5 Instituição 1 CPqGM Fiocruz/BA Centro de Pesquisas Gonçalo Moniz, Fiocruz – BA (Rua Waldemar Falcão, 121, Candeal Salvador/BA.), 2 UNB Universidade de Brasília – Laboratório de Parasitologia Médica e Biologia de Vetores (Campus Universitário Darcy Ribeiro, Brasília-DF.), 3 FRB-DeVry Faculdade Ruy Barbosa DeVry. (Rua Theodomiro Baptista, 422 Rio Vermelho, Salvador BA.), 4 UNIFACS Universidade Salvador (Rua Doutor José Peroba, no 251, Edf. Civil Empresarial, Sobreloja – STIEP, Salvador BA), 5 UFBA Universidade Federal da Bahia (Rua Augusto Viana, s/n Palácio da Reitoria, Canela, Salvador, BA.), 6 SESAB (DIVEP-...

Research paper thumbnail of Susceptibility characterization of residual Brazilian populations of Triatoma infestans Klug, 1834 (Hemiptera: Reduviidae) to deltamethrin pyrethroid

Revista da Sociedade Brasileira de Medicina Tropical, 2015

Introduction: Despite years of efforts towards the elimination of Triatoma infestans in Brazil, r... more Introduction: Despite years of efforts towards the elimination of Triatoma infestans in Brazil, residual foci still persist in some areas of the States of Bahia and Rio Grande do Sul. The persistence of these T. infestans populations in the country has two different origins of equal concern: operational failures or insecticide resistance. Thus, the objective of this study was to characterize the susceptibility profi le of the residual Brazilian populations of T. infestans to deltamethrin. Methods: The susceptibility reference lineage was derived from Cipein/Argentina. The populations studied were manually collected using a dislodging agent in peridomiciles in the States of Bahia (Novo Horizonte) and of Rio Grande do Sul (Santa Rosa and Doutor Maurício Cardoso). Serial dilutions of deltamethrin were prepared and applied at the dorsal abdomen of fi rst instar nymphs. The control group received only pure acetone. Mortality was evaluated after 72h. Qualitative tests assessed the mortality of a diagnostic dose of 1xLD 99 (2.76ng a.i./nymph) determined for the susceptibility reference lineage. Results: The susceptibility profi le characterization of the T. infestans populations revealed an RR 50 ranging from 1.73 to 3.26. The mortality percentage in response to a diagnostic dose was 100%. The results obtained in the quantitative and qualitative assays corresponded for all populations. Conclusions: The results of this study indicate that the persistence of residual foci of T. infestans in Bahia and Rio Grande do Sul is not related to insecticide resistance but may be associated with operational failures. In Rio Grande do Sul, we must consider the possibility of continuous reinfestation by Argentinian individuals, which justifi es active and effi cient epidemiological surveillance.

Research paper thumbnail of Prevalência e fatores associados às geo-helmintíases em crianças residentes em municípios com baixo IDH no Norte e Nordeste brasileiros

Cadernos De Saude Publica, 2010

Stool surveys were conducted to estimate the prevalence and identify risk factors for geohelminth... more Stool surveys were conducted to estimate the prevalence and identify risk factors for geohelminth infections among children in ten Brazilian municipalities with low human development indices (HDI). Socioeconomic and environmental data were obtained from the children's parents or guardians, and stool samples were examined. The proportion of geohelminth infections according to target variables was calculated. Risk factors were evaluated using multilevel logistic regression. Of the 2,523 children, 36.5% were infected with one or more geohelminths (Ascaris lumbricoides, 25.1%; hookworm, 15.3%; Trichuris trichiura, 12.2%). Overall prevalence of geohelminth infections was 45.7% in rural areas and 32.2% in urban areas. Low family income (OR = 1.75; 1.38-2.23), low maternal schooling (OR = 1.69; 1.39-2.06), presence of garbage near the home (OR = 1.50; 1.22-1.84), and number of individuals in the household (OR = 1.41; 1.17-1.71) were associated with infection. In conclusion, geohelminth infections were closely related to socioeconomic conditions, thus emphasizing the importance of targeted public interventions to improve living conditions as part of sustainable prevention.