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Papers by Edward Breitschwerdt

Research paper thumbnail of <i>Bartonella quintana</i>in Cynomolgus Monkey (<i>Macaca fascicularis</i>)

Emerging Infectious Diseases, Dec 1, 2005

Research paper thumbnail of Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

Parasites & Vectors, Mar 7, 2017

Research paper thumbnail of Coinfection with Three <i>Ehrlichia</i> Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure

Journal of Clinical Microbiology, 2001

Research paper thumbnail of Isolation and identification of Mycobacterium kansasii from pleural fluid of a dog with persistent pleural effusion

Javma-journal of The American Veterinary Medical Association, May 1, 2002

Research paper thumbnail of Development and Validation of a Sensitive and Specific <i>sodB</i> -Based Quantitative PCR Assay for Molecular Detection of Ehrlichia Species

Journal of Clinical Microbiology, Nov 1, 2014

Research paper thumbnail of Single-Tube Nested PCR for Detection of <i>Tritrichomonas</i> <i>foetus</i> in Feline Feces

Journal of Clinical Microbiology, Nov 1, 2002

Research paper thumbnail of Coinfection with Three Ehrlichia Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure

Journal of Clinical Microbiology, Oct 1, 2002

Research paper thumbnail of Capnocytophaga sp. Isolated from a Cat with Chronic Sinusitis and Rhinitis

Journal of Clinical Microbiology, Nov 1, 2003

Research paper thumbnail of Differentiation of Ehrlichia platys and E. equi Infections in Dogs by Using 16S Ribosomal DNA-Based PCR

Journal of Clinical Microbiology, Dec 1, 2001

Research paper thumbnail of Differentiation of Haemobartonella canis and Mycoplasma haemofelis on the basis of comparative analysis of gene sequences

American Journal of Veterinary Research, Oct 1, 2002

Research paper thumbnail of Infection and replication of Bartonella species within a tick cell line

Experimental and Applied Acarology, Feb 26, 2009

Research paper thumbnail of Prednisolone at anti-inflammatory or immunosuppressive dosages in conjunction with doxycycline does not potentiate the severity of Rickettsia rickettsii infection in dogs

Antimicrobial Agents and Chemotherapy, 1997

Research paper thumbnail of <i>Bartonella henselae</i> and <i>Bartonella elizabethae</i> as Potential Canine Pathogens

Journal of Clinical Microbiology, Dec 1, 2002

Research paper thumbnail of Bartonellosis: One Health Perspectives for an Emerging Infectious Disease

Research paper thumbnail of Bartonella species bacteremia in association with adult psychosis

Frontiers in psychiatry, Jun 7, 2024

Research paper thumbnail of <i>Bartonella</i>spp. Infection in Healthy and Sick Horses and Foals from the Southeastern United States

Journal of Veterinary Internal Medicine, Sep 24, 2012

Bartonella species bacteremia has been identified in numerous animal species. These bacteria caus... more Bartonella species bacteremia has been identified in numerous animal species. These bacteria cause, or have been associated with, a spectrum of clinical manifestations in dogs and human patients. The frequency of exposure to or infection with Bartonella spp. among healthy and sick horses has not been reported. To test healthy and sick horses and sick foals from the southeastern United States for serological, microbiological, and molecular evidence of Bartonella infection. Forty-seven healthy horses, 15 sick foals, 22 horses with musculoskeletal manifestations, and 8 horses with colic were tested for Bartonella. IFA serology and PCR before and after BAPGM (Bartonella alpha-Proteobacteria Growth Medium) enrichment blood culture. Bartonella antibodies were not detected in foals or horses. Three Bartonella species, B. henselae, B. vinsonii subsp. berkhoffii (genotypes I and III), and a Bartonella species with closest homology to Candidatus Bartonella volans, were PCR-amplified and sequenced from blood or BAPGM enrichment blood culture samples from 1/47 healthy horses, 3/15 sick foals, 5/22 horses with musculoskeletal disease, and 0/8 horses with colic. Horses in the southeastern United States are naturally infected with B. henselae, B. vinsonii subsp. berkhofii genotypes I and III, and a bacteria most similar to Candidatus Bartonella volans. Antibodies were not detectable by indirect fluorescent antibody assay (IFA) testing in bacteremic foals or horses, and prolonged enrichment culture for periods up to 21 days were necessary to document bacteremia in most horses. Further investigation into the pathogenic potential of Bartonella spp. infection in horses is warranted.

Research paper thumbnail of Experimental Ehrlichia canis infection in the dog does not cause immunosuppression

Veterinary Immunology and Immunopathology, 2006

Research paper thumbnail of Doxycycline Clearance of Experimentally Induced Chronic Ehrlichia Canis Infection in Dogs

Journal of Veterinary Internal Medicine, 2007

Ineffective clearance of Ehrlichia canis after doxycycline administration has been reported despi... more Ineffective clearance of Ehrlichia canis after doxycycline administration has been reported despite the fact that the recommended treatment for canine ehrlichiosis is doxycycline. The effectiveness of doxycycline in clearing E canis infection from the blood and tissues of dogs requires additional evaluation. Doxycycline (5 mg/kg PO q12h), administered for 4 weeks, will eliminate E canis infection from the blood and tissues of experimentally infected dogs. Fifteen Walker hound-mixed breed dogs were inoculated subcutaneously with E canis-infected canine histiocytic cells 4 months before doxycycline treatment. Four dogs were treated with doxycycline (5 mg/kg PO q12h for 3 weeks), 5 dogs were treated with doxycycline at the same dosage for 4 weeks, and 5 control dogs were not treated. Dexamethasone (0.4 mg/kg i.v.) was given after treatment to precipitate recrudescence of any remaining E canis organisms. Platelet counts, anti-E canis immunofluorescent antibodies, and polymerase chain reaction (PCR) detection of E canis deoxyribonucleic acid (DNA) in blood and tissues were evaluated. E canis DNA was not detected in the blood and tissues of doxycycline-treated dogs after treatment. Platelet counts were within reference intervals, and E canis antibodies decreased. Spontaneous clearance of E canis infection occurred in 2 of 5 control dogs. Three control dogs had E canis DNA detected in blood and tissues, platelet counts remained low or within the reference interval, and E canis antibodies remained high. As administered in this study, doxycycline cleared E canis from the blood and tissues of experimentally infected dogs.

Research paper thumbnail of Immunodiagnosis of Ehrlichia canis Infection with Recombinant Proteins

Journal of Clinical Microbiology, 2001

Research paper thumbnail of Visceral Leishmaniasis in a New York Foxhound Kennel

Journal of Veterinary Internal Medicine, 2002

Although endemic throughout much of the world, autochthonous visceral leishmaniasis has been repo... more Although endemic throughout much of the world, autochthonous visceral leishmaniasis has been reported on only 3 previous occasions in North America. After diagnosis of visceral leishmaniasis in 4 foxhounds from a kennel in Dutchess County, New York (index kennel), serum and ethylenediamine‐tetraacetic acid (EDTA)‐anticoagulated blood were collected from the remaining 108 American or cross‐bred foxhounds in the index kennel and from 30 Beagles and Basset Hounds that were periodically housed in the index kennel. Samples were analyzed for antibodies to or DNA of tickborne disease pathogens and Leishmania spp. Most dogs had antibodies to Rickettsia spp., Ehrlichia spp., Babesia spp., or some combination of these pathogens but not to Bartonella vinsonii (berkhoffi). However, DNA of rickettsial, ehrlichial, or babesial agents was detected in only 9 dogs. Visceral leishmaniasis was diagnosed in 46 of 112 (41%) foxhounds from the index kennel but was not diagnosed in any of the Beagles and Basset Hounds. A positive Leishmania status was defined by 1 or more of the following criteria: a Leishmania antibody titer ≥1:64, positive Leishmania polymerase chain reaction (PCR), positive Leishmania culture, or identification of Leishmania amastigotes by cytology or histopathology. The species and zymodeme of Leishmania that infected the foxhounds was determined to be Leishmania infantum MON‐1 by isoenzyme electrophoresis. Foxhounds that were &gt;18 months of age or that had traveled to the southeastern United States were more likely to be diagnosed with visceral leishmaniasis. Transmission of Leishmania spp. in kennel outbreaks may involve exposure to an insect vector, direct transmission, or vertical transmission.

Research paper thumbnail of <i>Bartonella quintana</i>in Cynomolgus Monkey (<i>Macaca fascicularis</i>)

Emerging Infectious Diseases, Dec 1, 2005

Research paper thumbnail of Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

Parasites & Vectors, Mar 7, 2017

Research paper thumbnail of Coinfection with Three <i>Ehrlichia</i> Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure

Journal of Clinical Microbiology, 2001

Research paper thumbnail of Isolation and identification of Mycobacterium kansasii from pleural fluid of a dog with persistent pleural effusion

Javma-journal of The American Veterinary Medical Association, May 1, 2002

Research paper thumbnail of Development and Validation of a Sensitive and Specific <i>sodB</i> -Based Quantitative PCR Assay for Molecular Detection of Ehrlichia Species

Journal of Clinical Microbiology, Nov 1, 2014

Research paper thumbnail of Single-Tube Nested PCR for Detection of <i>Tritrichomonas</i> <i>foetus</i> in Feline Feces

Journal of Clinical Microbiology, Nov 1, 2002

Research paper thumbnail of Coinfection with Three Ehrlichia Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure

Journal of Clinical Microbiology, Oct 1, 2002

Research paper thumbnail of Capnocytophaga sp. Isolated from a Cat with Chronic Sinusitis and Rhinitis

Journal of Clinical Microbiology, Nov 1, 2003

Research paper thumbnail of Differentiation of Ehrlichia platys and E. equi Infections in Dogs by Using 16S Ribosomal DNA-Based PCR

Journal of Clinical Microbiology, Dec 1, 2001

Research paper thumbnail of Differentiation of Haemobartonella canis and Mycoplasma haemofelis on the basis of comparative analysis of gene sequences

American Journal of Veterinary Research, Oct 1, 2002

Research paper thumbnail of Infection and replication of Bartonella species within a tick cell line

Experimental and Applied Acarology, Feb 26, 2009

Research paper thumbnail of Prednisolone at anti-inflammatory or immunosuppressive dosages in conjunction with doxycycline does not potentiate the severity of Rickettsia rickettsii infection in dogs

Antimicrobial Agents and Chemotherapy, 1997

Research paper thumbnail of <i>Bartonella henselae</i> and <i>Bartonella elizabethae</i> as Potential Canine Pathogens

Journal of Clinical Microbiology, Dec 1, 2002

Research paper thumbnail of Bartonellosis: One Health Perspectives for an Emerging Infectious Disease

Research paper thumbnail of Bartonella species bacteremia in association with adult psychosis

Frontiers in psychiatry, Jun 7, 2024

Research paper thumbnail of <i>Bartonella</i>spp. Infection in Healthy and Sick Horses and Foals from the Southeastern United States

Journal of Veterinary Internal Medicine, Sep 24, 2012

Bartonella species bacteremia has been identified in numerous animal species. These bacteria caus... more Bartonella species bacteremia has been identified in numerous animal species. These bacteria cause, or have been associated with, a spectrum of clinical manifestations in dogs and human patients. The frequency of exposure to or infection with Bartonella spp. among healthy and sick horses has not been reported. To test healthy and sick horses and sick foals from the southeastern United States for serological, microbiological, and molecular evidence of Bartonella infection. Forty-seven healthy horses, 15 sick foals, 22 horses with musculoskeletal manifestations, and 8 horses with colic were tested for Bartonella. IFA serology and PCR before and after BAPGM (Bartonella alpha-Proteobacteria Growth Medium) enrichment blood culture. Bartonella antibodies were not detected in foals or horses. Three Bartonella species, B. henselae, B. vinsonii subsp. berkhoffii (genotypes I and III), and a Bartonella species with closest homology to Candidatus Bartonella volans, were PCR-amplified and sequenced from blood or BAPGM enrichment blood culture samples from 1/47 healthy horses, 3/15 sick foals, 5/22 horses with musculoskeletal disease, and 0/8 horses with colic. Horses in the southeastern United States are naturally infected with B. henselae, B. vinsonii subsp. berkhofii genotypes I and III, and a bacteria most similar to Candidatus Bartonella volans. Antibodies were not detectable by indirect fluorescent antibody assay (IFA) testing in bacteremic foals or horses, and prolonged enrichment culture for periods up to 21 days were necessary to document bacteremia in most horses. Further investigation into the pathogenic potential of Bartonella spp. infection in horses is warranted.

Research paper thumbnail of Experimental Ehrlichia canis infection in the dog does not cause immunosuppression

Veterinary Immunology and Immunopathology, 2006

Research paper thumbnail of Doxycycline Clearance of Experimentally Induced Chronic Ehrlichia Canis Infection in Dogs

Journal of Veterinary Internal Medicine, 2007

Ineffective clearance of Ehrlichia canis after doxycycline administration has been reported despi... more Ineffective clearance of Ehrlichia canis after doxycycline administration has been reported despite the fact that the recommended treatment for canine ehrlichiosis is doxycycline. The effectiveness of doxycycline in clearing E canis infection from the blood and tissues of dogs requires additional evaluation. Doxycycline (5 mg/kg PO q12h), administered for 4 weeks, will eliminate E canis infection from the blood and tissues of experimentally infected dogs. Fifteen Walker hound-mixed breed dogs were inoculated subcutaneously with E canis-infected canine histiocytic cells 4 months before doxycycline treatment. Four dogs were treated with doxycycline (5 mg/kg PO q12h for 3 weeks), 5 dogs were treated with doxycycline at the same dosage for 4 weeks, and 5 control dogs were not treated. Dexamethasone (0.4 mg/kg i.v.) was given after treatment to precipitate recrudescence of any remaining E canis organisms. Platelet counts, anti-E canis immunofluorescent antibodies, and polymerase chain reaction (PCR) detection of E canis deoxyribonucleic acid (DNA) in blood and tissues were evaluated. E canis DNA was not detected in the blood and tissues of doxycycline-treated dogs after treatment. Platelet counts were within reference intervals, and E canis antibodies decreased. Spontaneous clearance of E canis infection occurred in 2 of 5 control dogs. Three control dogs had E canis DNA detected in blood and tissues, platelet counts remained low or within the reference interval, and E canis antibodies remained high. As administered in this study, doxycycline cleared E canis from the blood and tissues of experimentally infected dogs.

Research paper thumbnail of Immunodiagnosis of Ehrlichia canis Infection with Recombinant Proteins

Journal of Clinical Microbiology, 2001

Research paper thumbnail of Visceral Leishmaniasis in a New York Foxhound Kennel

Journal of Veterinary Internal Medicine, 2002

Although endemic throughout much of the world, autochthonous visceral leishmaniasis has been repo... more Although endemic throughout much of the world, autochthonous visceral leishmaniasis has been reported on only 3 previous occasions in North America. After diagnosis of visceral leishmaniasis in 4 foxhounds from a kennel in Dutchess County, New York (index kennel), serum and ethylenediamine‐tetraacetic acid (EDTA)‐anticoagulated blood were collected from the remaining 108 American or cross‐bred foxhounds in the index kennel and from 30 Beagles and Basset Hounds that were periodically housed in the index kennel. Samples were analyzed for antibodies to or DNA of tickborne disease pathogens and Leishmania spp. Most dogs had antibodies to Rickettsia spp., Ehrlichia spp., Babesia spp., or some combination of these pathogens but not to Bartonella vinsonii (berkhoffi). However, DNA of rickettsial, ehrlichial, or babesial agents was detected in only 9 dogs. Visceral leishmaniasis was diagnosed in 46 of 112 (41%) foxhounds from the index kennel but was not diagnosed in any of the Beagles and Basset Hounds. A positive Leishmania status was defined by 1 or more of the following criteria: a Leishmania antibody titer ≥1:64, positive Leishmania polymerase chain reaction (PCR), positive Leishmania culture, or identification of Leishmania amastigotes by cytology or histopathology. The species and zymodeme of Leishmania that infected the foxhounds was determined to be Leishmania infantum MON‐1 by isoenzyme electrophoresis. Foxhounds that were &gt;18 months of age or that had traveled to the southeastern United States were more likely to be diagnosed with visceral leishmaniasis. Transmission of Leishmania spp. in kennel outbreaks may involve exposure to an insect vector, direct transmission, or vertical transmission.