Edward Krainer - Academia.edu (original) (raw)

Papers by Edward Krainer

Microbiology, May 1, 1995

ChemInform, Jun 15, 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Tetrahedron Letters, 1999

Microbiology, 1995

SUMMARY A Candida albicans peptide transport gene, CaPTR2, was cloned from a C. albicans genomic ... more SUMMARY A Candida albicans peptide transport gene, CaPTR2, was cloned from a C. albicans genomic library by functional complementation of a peptide transport deficient mutant (strain ptr2-2) of Saccharomyces cerevisiae. CaPTR2 restored peptide transport to transformants as determined by uptake of radiolabelled dileucine, growth on dipeptides as sources of required amino acids, and restoration of growth inhibition by toxic peptides. Plasmid curing experiments demonstrated that the peptide transport phenotype was plasmid borne. CaPTR2 was localized to chromosome R of C. albicans by contour-clamped homologous electric field gel chromosome blots. Deletion subclones and frameshift mutagenesis were used to narrow the peptide transport complementing region to a 5:1 kb DNA fragment. DNA sequencing of the complementing region identified an ORF of 1869 bp containing an 84 nucleotide intron. The deduced amino acid sequence predicts a protein of 70 kDa consisting of 623 amino acids with 12 hydr...

A method of preparing a stabilizer complex which comprises reacting: a) a ketone of structure: wh... more A method of preparing a stabilizer complex which comprises reacting: a) a ketone of structure: where R1 and R2 are selected from the group consisting of H, methyl, C1-C20 alkyl, C2-C20, aryl, aryl is mono- or poly-substituted with C1-C15, C1-C4alkoxy and phenyl, b) an ester of the structure: wherein R4 is selected from the group consisting of C1-C5 alkyl, aryl and aryl which is substituted with C1-C4 alkyl, and R3 is selected from the group consisting of aryl, substituted aryl, C1-C20 alkyl and C2-C20 alkenyl; c) a base selected from the group consisting of calcium oxide, calcium hydroxide, barium oxide, barium hydroxide, potassium hydroxide, potassium oxide, sodium hydroxide and sodium oxide in the presence of a solvent selected from the group consisting of an aromatic hydrocarbon, an ether alkyl or aryl, dialkylsulfoxide, or the same ester used for the reaction, to form a reaction mixture containing a stabilizer complex; d) contacting the reaction mixture containing the stabilizer...

Journal of Medicinal Chemistry, 1991

Nine analogues (1-5, 9-12) of the peptidyl nucleoside antibiotics nikkomycin and polyoxin were sy... more Nine analogues (1-5, 9-12) of the peptidyl nucleoside antibiotics nikkomycin and polyoxin were synthesized and tested for their biological properties against different strains of the pathogenic yeast Candida albicans. The tripeptidyl series of analogues (1-5) was designed to behave as prodrugs, releasing a toxic moiety upon enzymatic hydrolysis inside the cell. The dipeptidyl series (9-12) was designed as double-targeted drugs, being themselves toxic and releasing a toxic amino acid upon hydrolysis. All the analogues were prepared by coupling suitably protected amino acid p-nitrophenyl esters to 1-(5'-amino-5'-deoxy-alpha-D-allofuranuronosyl)uracil (UPOC) or the corresponding polyoxins and nikkomycins, with subsequent removal of the protecting group. Improved coupling yields were observed when DMSO was used as the solvent. Products were purified with use of reversed-phase HPLC and, in one case, diastereomeric products (compound 11) were resolved by using this procedure. One of the tripeptidyl nikkomycins behaved as a prodrug but none of the compounds, as measured by in vitro testing, proved more effective than nikkomycin as an anticandidal agent.