El Sayed K A M A L A B D E L R A H M A N Mohamed (original) (raw)

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Papers by El Sayed K A M A L A B D E L R A H M A N Mohamed

Antibacterial Activity of Doxycycline against Aeromonas hydrophila in Experimentally Challenged African Catfish (Clarias gariepinus), 2019

Bacterial pathogens are the most serious agents causing diseases in both wild and cultured fish r... more Bacterial pathogens are the most serious agents causing diseases in both wild and cultured fish resulted in massive mortalities and economic losses. Motile Aeromonas Septicemia (MAS) is a prevalent bacterial disease caused by Aeromonas hydrophila (A. hydrophila) that impacts freshwater fish. This research aimed to evaluate doxycycline (DOX) antibacterial activity against A. hydrophila both in vitro and in vivo. The minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of DOX against A. hydrophila previously isolated from African catfish (Clarias gariepinus) were determined to be 0.78µg/mL and 3.9μg/mL, respectively. For in vivo experiment, a total number of 80 apparently healthy African catfish, were distributed randomly into four equal groups. Group 1 (non-infected, non-treated) was kept as control, Group 2 (non-infected and treated) was non-infected and treated with 20 mg/Kg BW of DOX for 5 successive days in feed, Group 3 (infected) was inoculated intraperitoneally (IP) with A. hydrophila (2× 10 8 CFU/ mL) and Group 4 (infected and treated) was infected with A. hydrophila then treated with 20 mg DOX/Kg BW. Our results revealed 70% mortality in African catfish experimentally challenged with A. hydrophila (Group 3). Moreover, significant elevation of serum alanine aminotransferase (ALT) (89±16.26, 54.67±6.44, 36±5.29 U/L, respectively), aspartate aminotransferase (AST) (195±7.64, 221.33±17.9, 211.33±12.72 U/L, respectively) and creatinine (0.68±0.098, 0.76±0.052, 0.58 ±0.023 mg/dL, respectively) was observed on 1 st , 7 th and 14 th days post treatment. While treatment of the infected fish (Group 4) with DOX decreased the mortality rate to 30 %, improved the clinical signs and significantly reduced serum ALT (30.67±6.01, 22.67±1.86 U/L, respectively) and AST (153±7.57, 147.67±6.7 U/L, respectively) on 7 th and 14 th days post treatment. Also, it significantly decreased creatinine (0.21±0.026, 0.25±0.047, 0.21±0.053 mg/dL, respectively) levels at 1 st , 7 th and 14 th days post treatment when compared with those of Group 3. The results showed that DOX could be used as an effective treatment against A. hydrophila infection in African catfish with little adverse effects.

Pharmacokinetic properties of single doxycycline dose orally administered in the African catfish (Clarias gariepinus), 2019

African catfish (Clarias gariepinus) aquaculture has experienced widespread production and has la... more African catfish (Clarias gariepinus) aquaculture has experienced
widespread production and has lately gained considerable interest in Egypt.
Doxycycline (DOX) is used to control certain common fish's bacterial diseases,
such as Septicemia, Fin rot, Columnaris, and Tail Rot. Therefore, our
experiment was conducted to assess the pharmacokinetic properties of single
doxycycline dose (20 mg/kg BW) orally administered in the African catfish.
DOX plasma levels were measured using HPLC with a limit of detection nearly
0.035 µg/ ml, and then were undergoing compartmental analysis; a onecompartment model was detected. The doubled-peak phenomenon was
identified after oral administration and the 1st peak concentration (Cmax1) and the
2nd peak concentration (Cmax2) in plasma were 2.29±0.46 and 1.68±0.33µg/mL
at 1st and 8th h respectively, the absorption half-life (t1/2ka) was 0.045 h, the
elimination half-life (t1/2ke) was 5.81 h, systemic total body clearance (Cl) was
0.72 mL/h/kg, volume of distribution of the central compartment (Vd/F) was
5.74±1.11 L. These findings suggested that DOX was to some extent rapidly
absorbed, widely distributed, and slowly excreted; moreover, it could be
subjected to enterohepatic recycling.

Pharmacokinetic properties of single doxycycline dose orally administered in the African catfish (Clarias gariepinus), 2019

African catfish (Clarias gariepinus) aquaculture has experienced widespread production and has la... more African catfish (Clarias gariepinus) aquaculture has experienced
widespread production and has lately gained considerable interest in Egypt.
Doxycycline (DOX) is used to control certain common fish's bacterial diseases,
such as Septicemia, Fin rot, Columnaris, and Tail Rot. Therefore, our
experiment was conducted to assess the pharmacokinetic properties of single
doxycycline dose (20 mg/kg BW) orally administered in the African catfish.
DOX plasma levels were measured using HPLC with a limit of detection nearly
0.035 µg/ ml, and then were undergoing compartmental analysis; a onecompartment model was detected. The doubled-peak phenomenon was
identified after oral administration and the 1st peak concentration (Cmax1) and the
2nd peak concentration (Cmax2) in plasma were 2.29±0.46 and 1.68±0.33µg/mL
at 1st and 8th h respectively, the absorption half-life (t1/2ka) was 0.045 h, the
elimination half-life (t1/2ke) was 5.81 h, systemic total body clearance (Cl) was
0.72 mL/h/kg, volume of distribution of the central compartment (Vd/F) was
5.74±1.11 L. These findings suggested that DOX was to some extent rapidly
absorbed, widely distributed, and slowly excreted; moreover, it could be
subjected to enterohepatic recycling.

Pharmacokinetics Doxycycline dose HPLC African catfish (Clarias gariepinus) aquaculture has exper... more Pharmacokinetics Doxycycline dose HPLC African catfish (Clarias gariepinus) aquaculture has experienced widespread production and has lately gained considerable interest in Egypt. Doxycycline (DOX) is used to control certain common fish's bacterial diseases, such as Septicemia, Fin rot, Columnaris, and Tail Rot. Therefore, our experiment was conducted to assess the pharmacokinetic properties of single doxycycline dose (20 mg/kg BW) orally administered in the African catfish. DOX plasma levels were measured using HPLC with a limit of detection nearly 0.035 µg/ ml, and then were undergoing compartmental analysis; a onecompartment model was detected. The doubled-peak phenomenon was identified after oral administration and the 1 st peak concentration (C max1) and the 2 nd peak concentration (C max2) in plasma were 2.29±0.46 and 1.68±0.33µg/mL at 1 st and 8 th h respectively, the absorption half-life (t 1/2ka) was 0.045 h, the elimination half-life (t 1/2ke) was 5.81 h, systemic total body clearance (Cl) was 0.72 mL/h/kg, volume of distribution of the central compartment (V d /F) was 5.74±1.11 L. These findings suggested that DOX was to some extent rapidly absorbed, widely distributed, and slowly excreted; moreover, it could be subjected to enterohepatic recycling.

Antibacterial Activity of Doxycycline against Aeromonas hydrophila in Experimentally Challenged African Catfish (Clarias gariepinus), 2019

Bacterial pathogens are the most serious agents causing diseases in both wild and cultured fish r... more Bacterial pathogens are the most serious agents causing diseases in both wild and cultured fish resulted in massive mortalities and economic losses. Motile Aeromonas Septicemia (MAS) is a prevalent bacterial disease caused by Aeromonas hydrophila (A. hydrophila) that impacts freshwater fish. This research aimed to evaluate doxycycline (DOX) antibacterial activity against A. hydrophila both in vitro and in vivo. The minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of DOX against A. hydrophila previously isolated from African catfish (Clarias gariepinus) were determined to be 0.78µg/mL and 3.9μg/mL, respectively. For in vivo experiment, a total number of 80 apparently healthy African catfish, were distributed randomly into four equal groups. Group 1 (non-infected, non-treated) was kept as control, Group 2 (non-infected and treated) was non-infected and treated with 20 mg/Kg BW of DOX for 5 successive days in feed, Group 3 (infected) was inoculated intraperitoneally (IP) with A. hydrophila (2× 10 8 CFU/ mL) and Group 4 (infected and treated) was infected with A. hydrophila then treated with 20 mg DOX/Kg BW. Our results revealed 70% mortality in African catfish experimentally challenged with A. hydrophila (Group 3). Moreover, significant elevation of serum alanine aminotransferase (ALT) (89±16.26, 54.67±6.44, 36±5.29 U/L, respectively), aspartate aminotransferase (AST) (195±7.64, 221.33±17.9, 211.33±12.72 U/L, respectively) and creatinine (0.68±0.098, 0.76±0.052, 0.58 ±0.023 mg/dL, respectively) was observed on 1 st , 7 th and 14 th days post treatment. While treatment of the infected fish (Group 4) with DOX decreased the mortality rate to 30 %, improved the clinical signs and significantly reduced serum ALT (30.67±6.01, 22.67±1.86 U/L, respectively) and AST (153±7.57, 147.67±6.7 U/L, respectively) on 7 th and 14 th days post treatment. Also, it significantly decreased creatinine (0.21±0.026, 0.25±0.047, 0.21±0.053 mg/dL, respectively) levels at 1 st , 7 th and 14 th days post treatment when compared with those of Group 3. The results showed that DOX could be used as an effective treatment against A. hydrophila infection in African catfish with little adverse effects.

Pharmacokinetic properties of single doxycycline dose orally administered in the African catfish (Clarias gariepinus), 2019

African catfish (Clarias gariepinus) aquaculture has experienced widespread production and has la... more African catfish (Clarias gariepinus) aquaculture has experienced
widespread production and has lately gained considerable interest in Egypt.
Doxycycline (DOX) is used to control certain common fish's bacterial diseases,
such as Septicemia, Fin rot, Columnaris, and Tail Rot. Therefore, our
experiment was conducted to assess the pharmacokinetic properties of single
doxycycline dose (20 mg/kg BW) orally administered in the African catfish.
DOX plasma levels were measured using HPLC with a limit of detection nearly
0.035 µg/ ml, and then were undergoing compartmental analysis; a onecompartment model was detected. The doubled-peak phenomenon was
identified after oral administration and the 1st peak concentration (Cmax1) and the
2nd peak concentration (Cmax2) in plasma were 2.29±0.46 and 1.68±0.33µg/mL
at 1st and 8th h respectively, the absorption half-life (t1/2ka) was 0.045 h, the
elimination half-life (t1/2ke) was 5.81 h, systemic total body clearance (Cl) was
0.72 mL/h/kg, volume of distribution of the central compartment (Vd/F) was
5.74±1.11 L. These findings suggested that DOX was to some extent rapidly
absorbed, widely distributed, and slowly excreted; moreover, it could be
subjected to enterohepatic recycling.

Pharmacokinetic properties of single doxycycline dose orally administered in the African catfish (Clarias gariepinus), 2019

African catfish (Clarias gariepinus) aquaculture has experienced widespread production and has la... more African catfish (Clarias gariepinus) aquaculture has experienced
widespread production and has lately gained considerable interest in Egypt.
Doxycycline (DOX) is used to control certain common fish's bacterial diseases,
such as Septicemia, Fin rot, Columnaris, and Tail Rot. Therefore, our
experiment was conducted to assess the pharmacokinetic properties of single
doxycycline dose (20 mg/kg BW) orally administered in the African catfish.
DOX plasma levels were measured using HPLC with a limit of detection nearly
0.035 µg/ ml, and then were undergoing compartmental analysis; a onecompartment model was detected. The doubled-peak phenomenon was
identified after oral administration and the 1st peak concentration (Cmax1) and the
2nd peak concentration (Cmax2) in plasma were 2.29±0.46 and 1.68±0.33µg/mL
at 1st and 8th h respectively, the absorption half-life (t1/2ka) was 0.045 h, the
elimination half-life (t1/2ke) was 5.81 h, systemic total body clearance (Cl) was
0.72 mL/h/kg, volume of distribution of the central compartment (Vd/F) was
5.74±1.11 L. These findings suggested that DOX was to some extent rapidly
absorbed, widely distributed, and slowly excreted; moreover, it could be
subjected to enterohepatic recycling.

Pharmacokinetics Doxycycline dose HPLC African catfish (Clarias gariepinus) aquaculture has exper... more Pharmacokinetics Doxycycline dose HPLC African catfish (Clarias gariepinus) aquaculture has experienced widespread production and has lately gained considerable interest in Egypt. Doxycycline (DOX) is used to control certain common fish's bacterial diseases, such as Septicemia, Fin rot, Columnaris, and Tail Rot. Therefore, our experiment was conducted to assess the pharmacokinetic properties of single doxycycline dose (20 mg/kg BW) orally administered in the African catfish. DOX plasma levels were measured using HPLC with a limit of detection nearly 0.035 µg/ ml, and then were undergoing compartmental analysis; a onecompartment model was detected. The doubled-peak phenomenon was identified after oral administration and the 1 st peak concentration (C max1) and the 2 nd peak concentration (C max2) in plasma were 2.29±0.46 and 1.68±0.33µg/mL at 1 st and 8 th h respectively, the absorption half-life (t 1/2ka) was 0.045 h, the elimination half-life (t 1/2ke) was 5.81 h, systemic total body clearance (Cl) was 0.72 mL/h/kg, volume of distribution of the central compartment (V d /F) was 5.74±1.11 L. These findings suggested that DOX was to some extent rapidly absorbed, widely distributed, and slowly excreted; moreover, it could be subjected to enterohepatic recycling.