Elaine Rodrigues - Academia.edu (original) (raw)

Uploads

Papers by Elaine Rodrigues

Revista Diálogo Educacional, 2019

Este artigo se insere no campo de pesquisa de história da educação no Paraná. A pesquisa toma a r... more Este artigo se insere no campo de pesquisa de história da educação no Paraná. A pesquisa toma a revista O Olho da Rua como fonte e objeto investigativo e o recorte temporal converge para o ciclo de vida do periódico, entre os anos de 1907 e 1911. Por meio de algumas charges veiculadas, o artigo então mostra como O Olho da Rua (1907-1911) se constituiu como um suporte material e meio de difusão do que era considerado retrógrado na instrução pública e no fazer prático da escola primária no início do século XX no estado do Paraná. Com base em quatro dessas charges sobre a instrução pública e os encaminhamentos práticos vigentes nas escolas primárias paranaenses, o principal dessa reflexão está direcionado para a seguinte problematização: − Como as charges de O Olho da Rua propuseram interpretações sobre a instrução pública paranaense e sobre o fazer prático da escola primária? Para responder a essa problematização, a história cultural baliza a manipulação do corpus documental e a semió...

Biochemical Journal, 1995

An exocellular proteinase activity has been characterized in Paracoccidioides brasiliensis cultur... more An exocellular proteinase activity has been characterized in Paracoccidioides brasiliensis culture filtrates. Chromatographic analysis showed that the activity was eluted from an anion-exchange Resource Q column at 0.08-0.1 M NaCl, and by gel filtration near ovalbumin elution, in a single peak. Purification of the proteinase, however, was hampered by the low protein yield, in contrast to the high peptidase activity. Numerous chromogenic peptidyl p-nitroanilide derivatives and internally quenched fluorescent peptides, flanked by Abz (O-aminobenzoyl) and EDDnp (ethylenediaminedinitrophenyl), were tested as substrates. Cleavage was observed with Abz-MKRLTL-EDDnp, Abz-FRLVR-EDDnp, and Abz-PLGLLGR-EDDnp at Leu-Thr, Leu-Val and Leu-Leu/Leu-Gly bonds respectively as determined by isolation of the corresponding fragments by HPLC. Leucine at P1 seemed to be restrictive for the activity of the exocellular enzyme, but threonine (P'1) and leucine (P'2) in Abz-MKRLTL-EDDnp apparently wer...

Encyclopedia of Portal Technologies and Applications

Melanoma Research, 2004

Tumour cell lines and in vivo growing tumours are heterogeneous, comprising different cell clones... more Tumour cell lines and in vivo growing tumours are heterogeneous, comprising different cell clones. To understand why some cells primarily invade a tissue, while others are more apt to metastasize, several clones from the established B16F10-Nex2 cell line were isolated and 10 viable cells of each clone were injected intravenously into C57Bl/6 and Balb/c mice. Two cell clones (Nex2B and Nex2D) showed contrasting metastatic abilities. Clone 2D rather than clone 2B colonized the lungs of both mice after intravenous injection. Surprisingly, clone 2B grew more rapidly than 2D after subcutaneous implantation, significantly reducing the survival of injected mice. Clearly, dissociation between subcutaneous growth and metastatic ability was observed in clones from the same tumour cell lineage. Clone Nex2B continuously released proteolytic activity, including cathepsin B, and showed a greater capacity to invade Matrigel than clone Nex2D. Clone Nex2D accumulated cathepsins B, D and L intracellularly and released a moderate proteolytic activity in vitro that was inhibited with the time of incubation. E-64-treated Nex2B cells injected subcutaneously showed a significant delay in tumour development and increased survival of challenged animals. A similar result was obtained on treatment of clone 2B with chagasin, a cysteine proteinase inhibitor from Trypanosoma cruzi, even at 2 microM. Clone Nex2D was less sensitive to pretreatment with inhibitors of cysteine proteases for tumour development in vivo. Our results suggest that, in a tumour cell population, cells dissociate into metastatic and non-metastatic subtypes, and that release or accumulation of cathepsins can be a differential trait of these cells.

The scope of this article is to present exploratory research into factors that influence purchasi... more The scope of this article is to present exploratory research into factors that influence purchasing frequency in virtual bookstores in Brazil. Despite the growth in volume of sales via electronic commerce and recognition of the importance of purchasing frequency by clients for the profitability of companies, little research has been conducted into the factors that motivate purchasing frequency via the Internet in Brazil. A survey was drawn up, based on an interval scale of attitude measurement, assembling 33 potentially influential variables on purchasing frequency. The data, obtained from a sample of 84 people, was analyzed using exploratory factor analysis, revealing 12 items gathered into three influential factors on purchasing frequency in virtual bookstores: (i) services and convenience of the site, (ii) promotion, and (iii) price. In conclusion, after identification of these factors, three propositions for explaining purchasing frequency in virtual bookstores in Brazil are presented in order that they can be rigorously tested in future research. INTRODUCTION The retail sector is currently experiencing a period of profound turmoil. Concerned at missing out on new business opportunities, companies are investing in the Internet as an additional sales channel. However, it is almost impossible to predict if traditional strategies will be profitable when applied to the Internet. One thing is abundantly clear, namely that electronic commerce has altered the ground rules across the board with respect to competitive advantage in the retail world (Chan et al., 2001). For the consumer the main advantages of electronic commerce over traditional commerce are convenience, information and practicality. On the other hand, the main disadvantages of electronic commerce in comparison with normal retail stores are the waiting period for receiving the product and the impossibility of any physical handling of the merchandise prior to purchase (Laudon & Traver, 2006). For Reedy et al. (2003), the Internet causes an impact on commercial activity in the following ways: (i) by reducing the transaction cost and stimulating economic activity; (ii) by creating new commercial opportunities thanks to the extent, accessibility and speed of access to information; and (iii) by transferring the power of vendors to purchasers. This new relationship between companies and clients has created different consumer habits such that for companies to remain competitive in the arena of electronic commerce they have to rethink their marketing strategies. Corporate marketing strategies in the virtual retail world are not merely focused on the client, as it is necessary to develop a relationship based more on partnership (Siegel, 1999). Joia & Rodrigues Purchasing Frequency in Virtual Bookstores in Brazil 2 This need for a medium or long-term relationship with the client is explained by the importance that purchasing frequency has for the economic success of commercialization via the Web. The acquisition of clients on the Web is time consuming and, unless these clients make repeated purchases, profits become unviable (Reichheld, 2003). Thus, the scope of this article is to present an exploratory study into the factors that influence purchasing frequency of consumers via the Internet in Brazil, as, despite the number of people who make virtual purchases being on the increase and the clear potential of electronic commerce and the challenges involved, little research in Brazil has been conducted with respect to the factors that motivate frequency of purchases via the Internet. More specifically, this research will be conducted in the sector of virtual bookstores in Brazil. This sector was chosen as one of those growing the fastest in the world in terms of volume of sales via the Internet, and the great competitiveness that characterizes it (Turban et al., 2005). BIBLIOGRAPHICAL REVIEW Consumer Behavior on the Web For Brondmo (2002), relationships between the Client and the Company fall into a predictable cycle of four stages: (i) Awareness, (ii) Permission, (iii) Involvement, and (iv) Loyalty. In order to get the client's attention, it is necessary to have promotional actions that should be aligned to the marketing planning of the company and based on the type of relationship that one seeks to develop with the client (Brondmo, 2002). By facilitating dialog between company and client, the Internet makes it even more important to get the client's attention. The content of the site should be sufficiently attractive to lead people to want to visit, highlighting the importance of making relevant information available to clients (Cheung et al., 2003). Once the company has aroused the client's awareness, it should request permission for communication. The goal at this stage is to stimulate the interest of potential clients in establishing a relationship with the company (Moustakas et al. 2005; Sipior et al., 2004; Joines et al., 2003). At this stage, the…

Revista Diálogo Educacional, 2019

Este artigo se insere no campo de pesquisa de história da educação no Paraná. A pesquisa toma a r... more Este artigo se insere no campo de pesquisa de história da educação no Paraná. A pesquisa toma a revista O Olho da Rua como fonte e objeto investigativo e o recorte temporal converge para o ciclo de vida do periódico, entre os anos de 1907 e 1911. Por meio de algumas charges veiculadas, o artigo então mostra como O Olho da Rua (1907-1911) se constituiu como um suporte material e meio de difusão do que era considerado retrógrado na instrução pública e no fazer prático da escola primária no início do século XX no estado do Paraná. Com base em quatro dessas charges sobre a instrução pública e os encaminhamentos práticos vigentes nas escolas primárias paranaenses, o principal dessa reflexão está direcionado para a seguinte problematização: − Como as charges de O Olho da Rua propuseram interpretações sobre a instrução pública paranaense e sobre o fazer prático da escola primária? Para responder a essa problematização, a história cultural baliza a manipulação do corpus documental e a semió...

Biochemical Journal, 1995

An exocellular proteinase activity has been characterized in Paracoccidioides brasiliensis cultur... more An exocellular proteinase activity has been characterized in Paracoccidioides brasiliensis culture filtrates. Chromatographic analysis showed that the activity was eluted from an anion-exchange Resource Q column at 0.08-0.1 M NaCl, and by gel filtration near ovalbumin elution, in a single peak. Purification of the proteinase, however, was hampered by the low protein yield, in contrast to the high peptidase activity. Numerous chromogenic peptidyl p-nitroanilide derivatives and internally quenched fluorescent peptides, flanked by Abz (O-aminobenzoyl) and EDDnp (ethylenediaminedinitrophenyl), were tested as substrates. Cleavage was observed with Abz-MKRLTL-EDDnp, Abz-FRLVR-EDDnp, and Abz-PLGLLGR-EDDnp at Leu-Thr, Leu-Val and Leu-Leu/Leu-Gly bonds respectively as determined by isolation of the corresponding fragments by HPLC. Leucine at P1 seemed to be restrictive for the activity of the exocellular enzyme, but threonine (P'1) and leucine (P'2) in Abz-MKRLTL-EDDnp apparently wer...

Encyclopedia of Portal Technologies and Applications

Melanoma Research, 2004

Tumour cell lines and in vivo growing tumours are heterogeneous, comprising different cell clones... more Tumour cell lines and in vivo growing tumours are heterogeneous, comprising different cell clones. To understand why some cells primarily invade a tissue, while others are more apt to metastasize, several clones from the established B16F10-Nex2 cell line were isolated and 10 viable cells of each clone were injected intravenously into C57Bl/6 and Balb/c mice. Two cell clones (Nex2B and Nex2D) showed contrasting metastatic abilities. Clone 2D rather than clone 2B colonized the lungs of both mice after intravenous injection. Surprisingly, clone 2B grew more rapidly than 2D after subcutaneous implantation, significantly reducing the survival of injected mice. Clearly, dissociation between subcutaneous growth and metastatic ability was observed in clones from the same tumour cell lineage. Clone Nex2B continuously released proteolytic activity, including cathepsin B, and showed a greater capacity to invade Matrigel than clone Nex2D. Clone Nex2D accumulated cathepsins B, D and L intracellularly and released a moderate proteolytic activity in vitro that was inhibited with the time of incubation. E-64-treated Nex2B cells injected subcutaneously showed a significant delay in tumour development and increased survival of challenged animals. A similar result was obtained on treatment of clone 2B with chagasin, a cysteine proteinase inhibitor from Trypanosoma cruzi, even at 2 microM. Clone Nex2D was less sensitive to pretreatment with inhibitors of cysteine proteases for tumour development in vivo. Our results suggest that, in a tumour cell population, cells dissociate into metastatic and non-metastatic subtypes, and that release or accumulation of cathepsins can be a differential trait of these cells.

The scope of this article is to present exploratory research into factors that influence purchasi... more The scope of this article is to present exploratory research into factors that influence purchasing frequency in virtual bookstores in Brazil. Despite the growth in volume of sales via electronic commerce and recognition of the importance of purchasing frequency by clients for the profitability of companies, little research has been conducted into the factors that motivate purchasing frequency via the Internet in Brazil. A survey was drawn up, based on an interval scale of attitude measurement, assembling 33 potentially influential variables on purchasing frequency. The data, obtained from a sample of 84 people, was analyzed using exploratory factor analysis, revealing 12 items gathered into three influential factors on purchasing frequency in virtual bookstores: (i) services and convenience of the site, (ii) promotion, and (iii) price. In conclusion, after identification of these factors, three propositions for explaining purchasing frequency in virtual bookstores in Brazil are presented in order that they can be rigorously tested in future research. INTRODUCTION The retail sector is currently experiencing a period of profound turmoil. Concerned at missing out on new business opportunities, companies are investing in the Internet as an additional sales channel. However, it is almost impossible to predict if traditional strategies will be profitable when applied to the Internet. One thing is abundantly clear, namely that electronic commerce has altered the ground rules across the board with respect to competitive advantage in the retail world (Chan et al., 2001). For the consumer the main advantages of electronic commerce over traditional commerce are convenience, information and practicality. On the other hand, the main disadvantages of electronic commerce in comparison with normal retail stores are the waiting period for receiving the product and the impossibility of any physical handling of the merchandise prior to purchase (Laudon & Traver, 2006). For Reedy et al. (2003), the Internet causes an impact on commercial activity in the following ways: (i) by reducing the transaction cost and stimulating economic activity; (ii) by creating new commercial opportunities thanks to the extent, accessibility and speed of access to information; and (iii) by transferring the power of vendors to purchasers. This new relationship between companies and clients has created different consumer habits such that for companies to remain competitive in the arena of electronic commerce they have to rethink their marketing strategies. Corporate marketing strategies in the virtual retail world are not merely focused on the client, as it is necessary to develop a relationship based more on partnership (Siegel, 1999). Joia & Rodrigues Purchasing Frequency in Virtual Bookstores in Brazil 2 This need for a medium or long-term relationship with the client is explained by the importance that purchasing frequency has for the economic success of commercialization via the Web. The acquisition of clients on the Web is time consuming and, unless these clients make repeated purchases, profits become unviable (Reichheld, 2003). Thus, the scope of this article is to present an exploratory study into the factors that influence purchasing frequency of consumers via the Internet in Brazil, as, despite the number of people who make virtual purchases being on the increase and the clear potential of electronic commerce and the challenges involved, little research in Brazil has been conducted with respect to the factors that motivate frequency of purchases via the Internet. More specifically, this research will be conducted in the sector of virtual bookstores in Brazil. This sector was chosen as one of those growing the fastest in the world in terms of volume of sales via the Internet, and the great competitiveness that characterizes it (Turban et al., 2005). BIBLIOGRAPHICAL REVIEW Consumer Behavior on the Web For Brondmo (2002), relationships between the Client and the Company fall into a predictable cycle of four stages: (i) Awareness, (ii) Permission, (iii) Involvement, and (iv) Loyalty. In order to get the client's attention, it is necessary to have promotional actions that should be aligned to the marketing planning of the company and based on the type of relationship that one seeks to develop with the client (Brondmo, 2002). By facilitating dialog between company and client, the Internet makes it even more important to get the client's attention. The content of the site should be sufficiently attractive to lead people to want to visit, highlighting the importance of making relevant information available to clients (Cheung et al., 2003). Once the company has aroused the client's awareness, it should request permission for communication. The goal at this stage is to stimulate the interest of potential clients in establishing a relationship with the company (Moustakas et al. 2005; Sipior et al., 2004; Joines et al., 2003). At this stage, the…