Elena Esimbekova - Academia.edu (original) (raw)

Papers by Elena Esimbekova

Siberian Medical Review, 2016

Journal of Biotechnology, Oct 1, 2008

With the rapid development of life and environmental science, people are increasingly concerned a... more With the rapid development of life and environmental science, people are increasingly concerned about the development and application of various biological sensors based on life materials. Enzyme biosensor is one of them; owing to extensive applications, enhancing its analytical capabilities is being paid more and more attention (Wang et al., 2006). Biosensor immobilization technology is the core of its production, which not only maintains the inherent characteristics of biological activity units, but also avoids application defects of free modules. The immobilization technology of biological activity unit determines the stability, sensitivity and selectivity as well as the research and application value of biosensor. Recently, the immobilizing sol-gel technology has provided a new approach to the immobilization of enzymes (Jing et al., 2003). To study the fiber-sensor film applied in detecting Organophosphorus pesticide, methyltriethoxysilane was used as precursor, which was doped by FITC-AchE. Consequently, the excellent Acetylcholinesterase biology sensitivity film was prepared. Physical characteristics of the film and the sensitivity of organophosphorus pesticide were researched. In organophosphorus pesticide residue testing, the experimental results indicated that the linear measurement range could arrive at mol/L, moreover the detection limit is mol/L. Compared to the existing electrochemical biosensors and optical biosensors, it is higher by 1-2 grades (Doong and Tsai, 2001; Gao et al., 2005). Fiber sensor will be fabricated by transplanting the film on photonic crystal fiber. The sensor could be applied in biological/chemical research, clinical medicines, environmental protection, food inspection, biochemical preventive war field and so on. References

Luminescence, Jun 21, 2018

In the present study, we demonstrate a disposable luciferasebased microfluidic bioassay chip for ... more In the present study, we demonstrate a disposable luciferasebased microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after introduction of water sample and thus activate bioluminescent reaction. Limits of detection for copper(II) sulfate, 1,3-dihydroxybenzene and 1,4-benzoquinone of the proposed microfluidic biosensor measured 3 µM, 15 mM and 2 µM respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers.

Life, May 24, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Doklady Akademii Nauk, Nov 10, 2019

A concept of the comprehensive assessment of soil contamination is proposed. In this concept, the... more A concept of the comprehensive assessment of soil contamination is proposed. In this concept, the conclusion regarding the presence of toxic substances in a sample, which is being analyzed, is connected with the inhibition of enzymatic reactions that are responsible for various functions of a live organism, such as luminescence, respiration, etc., these functions are taken as test functions in classical bioassays, which are using live objects (luminous bacteria, daphnia, algae, etc.). The regularities of the impact of different classes of toxicants on the activity of individual enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects-markers of pollution. The set of enzymatic bioassays consists of three enzyme systems with maximum sensitivity to different classes of toxicants: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. The possibility of using enzymes instead of living organisms in the bioassay of natural complex systems is shown.

Ecology & Safety, 2018

This paper examines the general principles of bioluminescent enzymatic toxicity bioassays and des... more This paper examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent Enzyme System Technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH:FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multi-component reagent 'Enzymolum' has been developed, which contains the bacterial luciferase, NADH:FMN-oxidoreductase and their substrates, co-immobilized in starch or gelatin gel. 'Enzymolum' can be integrated as a biological module into the portable biodetectorbiosensor originally constructed for personal use. Based on the example of 'Enzymolum' and the algorithm of creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 minutes), high sensitivity, simplicity and safety of procedure, possibility of automation of ecological monitoring; required luminometer is easily available.

International Journal of Biological Macromolecules, Jun 1, 2019

The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90... more The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90 (Hsp90) without additional co-chaperone supplementation; intended for high-throughput screening of anti-cancer drugs and prognosis of stress. In this regard, Hsp90 and adenosine 5′-triphosphate (ATP) mediated firefly luciferase (FLuc) kinetics was investigated using apyrase and FLuc as client proteins. Bioluminescent assay containing Hsp90, ATP, and apyrase led to complete loss of luminescence at 50°C which indicates the protective role of Hsp90 against thermal denaturation. Similarly, the assay sample comprising Hsp90, ATP, and FLuc showed 2 fold increments in luminescence than their counterparts. Introduction of bovine serum albumin (BSA) to the pre-incubated assay mixture led to an initial rise in the luminescence (28%) in comparison to the sample containing Hsp90, ATP and FLuc. Therefore, FLuc based HTS assays are not suitable for clinical samples which may contain stabilizing agents. However, thermally denatured FLuc and apyrase could not regain their active conformation even when Hsp90 and ATP were introduced in the assay system. This observation justifies the role of Hsp90 to be protective rather than a reparation agent when acts without co-chaperones.

Doklady Biochemistry and Biophysics, 2017

A new method for assessing biotoxicity of nanomaterials, based on the use of soluble bioluminesce... more A new method for assessing biotoxicity of nanomaterials, based on the use of soluble bioluminescent coupled enzyme system NAD(P)⋅H:FMN oxidoreductase and luciferase, is proposed. The results of this study indicate a significant adverse biological effect exerted by nanoparticles at the molecular level. It was found that the most toxic nanoparticles the nanoparticles are based on copper and copper oxide, as well as single-walled carbon nanotubes and multi-walled carbon nanofibers, which are referred to hazard class II.

Doklady Biological Sciences, Nov 1, 2019

Sensors and Actuators B-chemical, Jul 1, 2015

ABSTRACT The biomodule of bioluminescent biosensor based on a coupled enzyme system NADH:FMN-oxid... more ABSTRACT The biomodule of bioluminescent biosensor based on a coupled enzyme system NADH:FMN-oxidoreductase and luciferase, co-immobilized with substrates in dried starch or gelatin gels, has been developed. We studied the impact of several stabilizers—dithiothreitol (DTT), bovine serum albumin (BSA) and mercaptoethanol (ME) on the biomodule's activity, storage stability and sensitivity to toxic substances. The inclusion of stabilizers increases the activity of the biological module by more than 150%. To achieve the combination of high activity, prolonged storage time and acute sensitivity to toxic substances within maximum permissible concentration we used starch gel as a carrier adding 100 μM DTT to the immobilized preparation. The gelatin-based biological module had greater storage stability than the starch-based one but demonstrated less sensitivity to toxic substances.

Food Chemistry, Nov 1, 2017

The present manuscript proposes a novel approach to assess the impact of food additives on human ... more The present manuscript proposes a novel approach to assess the impact of food additives on human metabolism by analysing their effect on biomarker enzyme activity. Alterations in the activity of pancreatic enzymes, such as chymotrypsin and trypsin, which are affected by the most common food preservatives, sodium benzoate (E211), potassium sorbate (E202) and sorbic acid (E200), have been evaluated. The proteinase activity was analysed with a bioluminescent method using the light intensity decay constant. Our study revealed that the preservatives reduce proteinase activity by 50% (EC 50) at a much lower concentration than their acceptable daily intake (ADI). Thus, sodium benzoate and sorbic acid have an inhibition effect on chymotrypsin at concentrations 14 times lower and 70 times lower than their ADI and this increases with exposure time. Food preservative consumption impacts negatively on protein digestion, which is especially dangerous for patients with pancreatitis.

Analytica Chimica Acta, Jun 1, 2017

Nanomaterials significantly differ from their bulk counterparts. Strong and pronounced photophysi... more Nanomaterials significantly differ from their bulk counterparts. Strong and pronounced photophysical effects at the metal surface provide opportunities for designing novel biosensors. Metal-enhanced luminescence increases the quantum yield of luminescent reactions. Under optimal conditions, plasmon coupling enhances the optical effects at the nanometal surface.

Biosensors and Bioelectronics, 2017

The present review critically discusses the latest developments in the field of smart diagnostic ... more The present review critically discusses the latest developments in the field of smart diagnostic systems for cancer biomarkers. A wide coverage of recent biosensing approaches involving aptamers, enzymes, DNA probes, fluorescent probes, interacting proteins and antibodies in vicinity to transducers such as electrochemical, optical and piezoelectric is presented. Recent advanced developments in biosensing approaches for cancer biomarker owes much credit to functionalized nanomaterials due to their unique opto-electronic properties and enhanced surface to volume ratio. Biosensing methods for a plenty of cancer biomarkers has been summarized emphasizing the key principles involved.

Журнал Сибирского федерального университета, Jun 1, 2017

The bioluminescent enzymatic bioassays for assessment of nanomaterial biotoxicity using the solub... more The bioluminescent enzymatic bioassays for assessment of nanomaterial biotoxicity using the soluble or immobilized coupled enzyme system of luminous bacteria NAD(P)Н:FMN-oxidoreductase + luciferase (Red + Luc) as a test system were employed in this study. This method specifically detects the toxic properties of substances based on their effect on the parameters of the bioluminescent enzyme reactions. The commercially available metal nanoparticles (MNPs), including silver nanoparticles (Ag), nanoparticles of silicon dioxide (SiO 2), and titanium dioxide (TiO 2), of different sizes were tested in the study. The inhibitory effects of MNPs on the bioluminescent Red + Luc enzyme system were measured. Results indicated that the soluble Red + Luc coupled enzyme system was more sensitive to the inhibition effect of MNPs than its immobilized form. The inhibitory activity of MNPs decreased in the following order: Ag > TiO 2 > SiO 2. That correlated well with results of other biological methods. Due to substantial advantages such as technical simplicity, short response time and high sensitivity to analysis, this bioluminescent enzymatic bioassay has the potential to be developed as a general bioassay for safety assessment of a wide variety of nanomaterials.

Catalysts, Apr 13, 2021

Combinatorial Chemistry & High Throughput Screening, Nov 3, 2015

This review describes the principle and applications of bioluminescent enzymatic toxicity bioassa... more This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMNoxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.

Micromachines, Jul 18, 2023