Eli SELLEM - Academia.edu (original) (raw)

Papers by Eli SELLEM

Research paper thumbnail of Multi-trait genetic parameter estimates in a Tenebrio molitor reference population: high potential for breeding gains

Research paper thumbnail of Additional file 8 of Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records

Research paper thumbnail of Additional file 9 of Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records

Research paper thumbnail of Additional file 5 of Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records

Research paper thumbnail of Intergenerational effects on fertility in male and female mice after chronic exposure to environmental doses of NSAIDs and 17α-ethinylestradiol mixtures

Food and Chemical Toxicology, Nov 30, 2023

Research paper thumbnail of ŸnFABRE: design of reference populations for genomic selection in Tenebrio molitor

HAL (Le Centre pour la Communication Scientifique Directe), Aug 25, 2023

Research paper thumbnail of Bull spermatozoa DNA methylation landscape displays unique features revealed by multi-scale analysis

HAL (Le Centre pour la Communication Scientifique Directe), Jan 9, 2016

Research paper thumbnail of 118 Contribution of sperm methylome to bull fertility and interactions with DNA polymorphism

Journal of Animal Science, Dec 1, 2018

Spermatozoa have a remarkable epigenome in line with their degree of specialization, their unique... more Spermatozoa have a remarkable epigenome in line with their degree of specialization, their unique nature and different requirements for successful fertilization. While bull semen is widely used in artificial insemination, the literature describing DNA methylation in bovine spermatozoa is still scarce. In the context of genomic selection, more information on the epigenetic features transferred to the embryo alongside the paternal genetic heritage is necessary, in order to improve semen quality control procedures and bull fertility. We characterized the bull sperm methylome relative to bovine fibroblasts and monocytes using reduced restriction bisulfite sequencing (RRBS). A wide majority of differentially methylated cytosines (DMCs) were less methylated in sperm than in somatic cells. Consistent with previous studies in other species, these hypomethylated DMCs were enriched for genes relevant to the germline differentiation program and sperm functions. The most remarkable observation was a dramatic enrichment for repeats and particularly for satellites, which may partly account for the lower global methylation we observed in bull sperm relative to sperm from other species (LUminometric Methylation Assay). We next investigated whether fertility biomarkers could be identified in the sperm methylome. Based on field fertility indicators, we compared fertile ejaculates, subfertile ejaculates and ejaculates from bulls with a disappointing career in contradiction with genomic predictions (Montbéliarde breed, n=10/group). The median age at production was consistent among groups and the phenotypic characterization revealed no significant differences except for the proportion of motile sperm, which was reduced in disappointing bulls. We identified 4823 DMCs targeting genes involved in signaling pathways, angiogenesis and the response to growth factors. Since DNA methylation is affected by the CpG content of the genome and its alteration by DNA polymorphism, an integrative approach was conducted to identify CpGs and SNPs in correlation, which revealed both direct and indirect interactions between the genome and the epigenome.

Research paper thumbnail of Phéromones de l’oestrus et potentialisation de la libido chez le taureau

HAL (Le Centre pour la Communication Scientifique Directe), Oct 3, 2013

National audienceLa communication chimique joue un rôle essentiel dans la mise en place de nombre... more National audienceLa communication chimique joue un rôle essentiel dans la mise en place de nombreux comportements chez les mammifères et en particulier du comportement sexuel. Ainsi, la femelle en oestrus attire le male via l’émission de phéromones dans ses urines. Afin d’identifier, les phéromones impliquées dans ce processus, plusieurs séries de collectes d’urine ont été réalisées à différents stades d’un cycle oestral sur des vaches synchronisées (N=30). La comparaison de leur composition chimique (déterminée par GC/MS) a permis de caractériser six molécules produites spécifiquement aux stades pré-oestrus et/ou oestrus. Cinq de ces molécules (le coumarin, le squalène, l’acide oléique, la 2-butanone, le 1,2-dichloro-éthylène), disponibles commercialement, ont été testées, seules ou en mélange, pour leur activité biologique sur les taureaux. Solubilisées à concentration physiologique (25 pg/ml) dans une solution de glycérol/eau (50/50), les différentes combinaisons ont été pulvérisées dans les cavités nasales des taureaux

Research paper thumbnail of Dual role of DMXL2 in olfactory information transmission and the first wave of spermatogenesis

PLOS Genetics, Feb 8, 2019

Research paper thumbnail of Methylation levels at the GNAS locus is not associated with field fertility in Holstein bulls

HAL (Le Centre pour la Communication Scientifique Directe), Apr 26, 2015

ABSTRACT

Research paper thumbnail of Small non coding RNA from frozen bull sperm cells: biomarkers of male fertility?

HAL (Le Centre pour la Communication Scientifique Directe), Jul 16, 2017

Research paper thumbnail of Small non coding RNA from Frozen bull sperm cells

HAL (Le Centre pour la Communication Scientifique Directe), Jun 26, 2016

Research paper thumbnail of Nuclear protein contents - An epigenetic marking of sperm cells

HAL (Le Centre pour la Communication Scientifique Directe), May 27, 2018

Research paper thumbnail of Les petits ARN non codants du spermatozoïde bovin, de potentiels biomarqueurs de la fertilité mâle ?

HAL (Le Centre pour la Communication Scientifique Directe), May 22, 2017

Research paper thumbnail of Bull spermatozoa methylation landscape displays breed-specific features revealed by RRBS analysis

HAL (Le Centre pour la Communication Scientifique Directe), Sep 14, 2016

Research paper thumbnail of Long-term impact of early life nutrition on DNA methylation patterns in bull sperm

HAL (Le Centre pour la Communication Scientifique Directe), Aug 26, 2019

Research paper thumbnail of Prediction of bull fertility based on the sperm methylome

HAL (Le Centre pour la Communication Scientifique Directe), 2021

International audienc

Research paper thumbnail of Epigenetics of bovine semen: tools to DNA methylation analysis

HAL (Le Centre pour la Communication Scientifique Directe), Apr 26, 2015

ABSTRACT

Research paper thumbnail of Absence of Testicular Estrogen Leads to Defects in Spermatogenesis and Increased Semen Abnormalities in Male Rabbits

Research paper thumbnail of Multi-trait genetic parameter estimates in a Tenebrio molitor reference population: high potential for breeding gains

Research paper thumbnail of Additional file 8 of Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records

Research paper thumbnail of Additional file 9 of Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records

Research paper thumbnail of Additional file 5 of Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records

Research paper thumbnail of Intergenerational effects on fertility in male and female mice after chronic exposure to environmental doses of NSAIDs and 17α-ethinylestradiol mixtures

Food and Chemical Toxicology, Nov 30, 2023

Research paper thumbnail of ŸnFABRE: design of reference populations for genomic selection in Tenebrio molitor

HAL (Le Centre pour la Communication Scientifique Directe), Aug 25, 2023

Research paper thumbnail of Bull spermatozoa DNA methylation landscape displays unique features revealed by multi-scale analysis

HAL (Le Centre pour la Communication Scientifique Directe), Jan 9, 2016

Research paper thumbnail of 118 Contribution of sperm methylome to bull fertility and interactions with DNA polymorphism

Journal of Animal Science, Dec 1, 2018

Spermatozoa have a remarkable epigenome in line with their degree of specialization, their unique... more Spermatozoa have a remarkable epigenome in line with their degree of specialization, their unique nature and different requirements for successful fertilization. While bull semen is widely used in artificial insemination, the literature describing DNA methylation in bovine spermatozoa is still scarce. In the context of genomic selection, more information on the epigenetic features transferred to the embryo alongside the paternal genetic heritage is necessary, in order to improve semen quality control procedures and bull fertility. We characterized the bull sperm methylome relative to bovine fibroblasts and monocytes using reduced restriction bisulfite sequencing (RRBS). A wide majority of differentially methylated cytosines (DMCs) were less methylated in sperm than in somatic cells. Consistent with previous studies in other species, these hypomethylated DMCs were enriched for genes relevant to the germline differentiation program and sperm functions. The most remarkable observation was a dramatic enrichment for repeats and particularly for satellites, which may partly account for the lower global methylation we observed in bull sperm relative to sperm from other species (LUminometric Methylation Assay). We next investigated whether fertility biomarkers could be identified in the sperm methylome. Based on field fertility indicators, we compared fertile ejaculates, subfertile ejaculates and ejaculates from bulls with a disappointing career in contradiction with genomic predictions (Montbéliarde breed, n=10/group). The median age at production was consistent among groups and the phenotypic characterization revealed no significant differences except for the proportion of motile sperm, which was reduced in disappointing bulls. We identified 4823 DMCs targeting genes involved in signaling pathways, angiogenesis and the response to growth factors. Since DNA methylation is affected by the CpG content of the genome and its alteration by DNA polymorphism, an integrative approach was conducted to identify CpGs and SNPs in correlation, which revealed both direct and indirect interactions between the genome and the epigenome.

Research paper thumbnail of Phéromones de l’oestrus et potentialisation de la libido chez le taureau

HAL (Le Centre pour la Communication Scientifique Directe), Oct 3, 2013

National audienceLa communication chimique joue un rôle essentiel dans la mise en place de nombre... more National audienceLa communication chimique joue un rôle essentiel dans la mise en place de nombreux comportements chez les mammifères et en particulier du comportement sexuel. Ainsi, la femelle en oestrus attire le male via l’émission de phéromones dans ses urines. Afin d’identifier, les phéromones impliquées dans ce processus, plusieurs séries de collectes d’urine ont été réalisées à différents stades d’un cycle oestral sur des vaches synchronisées (N=30). La comparaison de leur composition chimique (déterminée par GC/MS) a permis de caractériser six molécules produites spécifiquement aux stades pré-oestrus et/ou oestrus. Cinq de ces molécules (le coumarin, le squalène, l’acide oléique, la 2-butanone, le 1,2-dichloro-éthylène), disponibles commercialement, ont été testées, seules ou en mélange, pour leur activité biologique sur les taureaux. Solubilisées à concentration physiologique (25 pg/ml) dans une solution de glycérol/eau (50/50), les différentes combinaisons ont été pulvérisées dans les cavités nasales des taureaux

Research paper thumbnail of Dual role of DMXL2 in olfactory information transmission and the first wave of spermatogenesis

PLOS Genetics, Feb 8, 2019

Research paper thumbnail of Methylation levels at the GNAS locus is not associated with field fertility in Holstein bulls

HAL (Le Centre pour la Communication Scientifique Directe), Apr 26, 2015

ABSTRACT

Research paper thumbnail of Small non coding RNA from frozen bull sperm cells: biomarkers of male fertility?

HAL (Le Centre pour la Communication Scientifique Directe), Jul 16, 2017

Research paper thumbnail of Small non coding RNA from Frozen bull sperm cells

HAL (Le Centre pour la Communication Scientifique Directe), Jun 26, 2016

Research paper thumbnail of Nuclear protein contents - An epigenetic marking of sperm cells

HAL (Le Centre pour la Communication Scientifique Directe), May 27, 2018

Research paper thumbnail of Les petits ARN non codants du spermatozoïde bovin, de potentiels biomarqueurs de la fertilité mâle ?

HAL (Le Centre pour la Communication Scientifique Directe), May 22, 2017

Research paper thumbnail of Bull spermatozoa methylation landscape displays breed-specific features revealed by RRBS analysis

HAL (Le Centre pour la Communication Scientifique Directe), Sep 14, 2016

Research paper thumbnail of Long-term impact of early life nutrition on DNA methylation patterns in bull sperm

HAL (Le Centre pour la Communication Scientifique Directe), Aug 26, 2019

Research paper thumbnail of Prediction of bull fertility based on the sperm methylome

HAL (Le Centre pour la Communication Scientifique Directe), 2021

International audienc

Research paper thumbnail of Epigenetics of bovine semen: tools to DNA methylation analysis

HAL (Le Centre pour la Communication Scientifique Directe), Apr 26, 2015

ABSTRACT

Research paper thumbnail of Absence of Testicular Estrogen Leads to Defects in Spermatogenesis and Increased Semen Abnormalities in Male Rabbits