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Papers by Eliana Endo

Journal de Mycologie Médicale

Ketoconazole-loaded poly-(lactic acid) nanoparticles: characterization and improvement of antifun... more Ketoconazole-loaded poly-(lactic acid) nanoparticles: characterization and improvement of antifungal efficacy in vitro against Candida and dermatophytes

Brazilian Journal of Microbiology

Endophytes are microorganisms that form symbiotic relationships with their host. These microorgan... more Endophytes are microorganisms that form symbiotic relationships with their host. These microorganisms can produce a variety of secondary metabolites, some of which have inhibitory effects on pests and pathogens or even act to promote plant growth. Due to these characteristics, these microorganisms are used as sources of biologically active substances for a wide range of biotechnological applications. Based on that, the aim of this study was to evaluate the production of metabolites of the endophytic Aspergillus flavus CL7 isolated from Chromolaena laevigata, in four different cultivation conditions, and to determine the antimicrobial, cytotoxic, antiviral, and antioxidant potential of these extracts. The multiphasic approach used to identify this strain was based on morphology and ITS gene sequence analysis. The chemical investigation of A. flavus using potato dextrose and minimal medium, using both stationary and agitated methods, resulted in the isolation of kojic acid, α-cyclopiazonic acid, and 20,25-dihydroxyaflavinine. Another 18 compounds in these extracts were identified by UHPLC-HRMS/MS, of which dideacetyl parasiticolide A has been described for the first time from A. flavus. Aflatoxins, important chemomarkers of A. flavus, were not detected in any of the extracts, thus indicating that the CL7 strain is non-aflatoxigenic. The biological potential of all extracts was evaluated, and the best results were observed for the extract obtained using minimal medium against Trichophyton rubrum and Mycobacterium tuberculosis.

Phytochemical investigation of Chromolaena laevigata led to the isolation of a... more Phytochemical investigation of Chromolaena laevigata led to the isolation of a new cadinene-sesquiterpene, chromolaevigone glucoside (1), along with nine known compounds: daucosterol (2), stigmasterol glycoside (3), stigmasterol (4), β-sitosterol (5), pilloin (6), gonzalitosin I (7), quercetin-3-O-α-rhamnopyranoside (8), 7,7-dihydroxy-calamen-12-oic acid lactone (9) and trachelanthic acid (10). Others 11 known compounds were identified by UHPLC-HRMS/MS. These compounds are being described for the first time in this species, with the exception of cadinene 9. Furthermore, due to the limitation of pharmacological studies, antiproliferative, antiviral, and antimicrobial activities of C. laevigata were evaluated. The best resu...

Research, Society and Development, 2021

Alicyclobacillus spp. is composed of Gram-positive, aerobic, thermoacidophilic, endospore-forming... more Alicyclobacillus spp. is composed of Gram-positive, aerobic, thermoacidophilic, endospore-forming bacteria that cause food and beverage spoilage. The presence of Alicyclobacillus spp. may result in the production of guaiacol, which leads to sensory changes in the odour and taste of citrus juices and acidic foods. Tabernaemontana catharinensis (A. DC) is a plant belonging to the family Apocynaceae that produces milky latex with several biological activities described as antioxidant, antiviral, antimicrobial, trypanocidal and anti-leishmanicidal. Therefore, this study aims to evaluate the antimicrobial activity of the crude latex of T. catharinensis (A. DC) against microorganisms of the genus Alicyclobacillus spp. The minimum inhibitory concentration of latex was 7.81 μg/ml for the five Alicyclobacillus species analysed. The minimum bactericidal concentration for the species Alicyclobacillus acidoterrestris 0244T, A. hesperidum 0298T, A. acidiphilus 0247T and A. cycloheptanicus 0297T ...

Letters in Applied Microbiology, 2020

Significance and Impact of the Study: Alicyclobacillus sp. are responsible for the deterioration ... more Significance and Impact of the Study: Alicyclobacillus sp. are responsible for the deterioration of orange juice, mainly due to their capacity to form spores. In the search for new control alternatives, this study evaluated the antimicrobial activity of rosemary essential oil and its combination with nisin against the vegetative cells and spores of Alicyclobacillus acidoterrestris in orange juice. The phenolic compound content and antioxidant activity of orange juice were also evaluated after the addition of these compounds. Rosemary oil, when associated with nisin, resulted in a sporicidal effect, thus demonstrating its potential application in the prevention of Alicyclobacillus sp. in orange juice.

Journal de Mycologie Médicale, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Postharvest Biology and Technology, 2018

The occurrence of mycotoxins synthesized by spoilage fungi has been reported in various crops. De... more The occurrence of mycotoxins synthesized by spoilage fungi has been reported in various crops. Despite being a crop susceptible to attack by microorganisms, such as Alternaria alternata, there are no reports on the production of mycotoxins in Hylocereus undatus (Haw.). The objective of the present study was to identify mycotoxins produced by A. alternata previously isolated from Hylocereus undatus (Haw.) fruit in the postharvest phase, and A. alternata ATCC 46,582, in vitro and in vivo. The compounds were identified using liquid chromatography-mass spectrometry (LC-MS/MS). Alternariol (AOH), alternariol monomethyl ether (AME), tentoxin (TEN), altertoxins (ATX) I, II and III were detected in vitro for A. alternata, and ATX I and II were identified for the reference strain, A. alternata ATCC 46,582, which was used as a positive control. Mycotoxins characteristic of A. alternata were not detected in vivo in the fruit stored for 8 d, which is its estimated shelf life. The use of LC-MS/MS facilitated rapid and efficient mycotoxin identification, contributing significantly to the evaluation of food safety.

Journal of the Brazilian Chemical Society, 2016

Copaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicin... more Copaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 μg mL-1 (250 μg mL-1), 500 μg mL-1 (500 μg mL-1) and 250 μg mL-1 (250 μg mL-1), respectively. For copalic acid, the MIC and MFC were 50 μg mL-1 (100 μg mL-1), 100 μg mL-1 (100 μg mL-1) and 50 μg mL-1 (100 μg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs.

Industrial Crops and Products, 2017

This study isolated and identified the fungus that causes postharvest disease in dragon fruit (Hy... more This study isolated and identified the fungus that causes postharvest disease in dragon fruit (Hylocereus undatus Haw.). The in vitro and in vivo antifungal activity of some essential oils were evaluated against the fungus. Morphophysiological and molecular identification confirmed the fungus was Alternaria alternata. The essential oils of Cinnamomum zeylanicum, Cymbopogon flexuosus, Eucalyptus globulus, Eugenia caryophyllus, and Rosmarinus officinalis were evaluated by the microdilution broth technique, disc diffusion, scanning electron microscopy, and fluorescence microscopy. Evaluation of the composition of the essential oils by gas chromatography/mass spectrometry revealed substantial amounts of eugenol as a major constituent of E. caryophyllus and C. zeylanicum (90.50% and 80.70%, respectively). The other essential oils of R. officinalis contained ␣-pinene (24.5%) and camphor (22.0%) as major components. E. globulus contained 1,8-cineole (78.9%). C. flexuosus contained neral (35.1%) and geranial (42.6%). C. zeylanicum and E. caryophyllus were the most active against the isolated fungi at minimum inhibitory concentrations of 250 and 500 g/ml, respectively, causing morphological changes in the hyphae. The in vivo assay indicated that the fruits that were treated with E. caryophyllus at concentrations of 500 and 1000 g/ml exhibited a 31% reduction of mycelial growth compared with the control. These results suggest that the essential oils of C. zeylanicum and E. caryophyllus are active against A. alternata both in vitro and in vivo, which may be promising for control of the microorganism.

Revista Brasileira de Farmacognosia, 2017

Many infections worldwide are associated with bacterial biofilms. The effects of isolated neolign... more Many infections worldwide are associated with bacterial biofilms. The effects of isolated neolignans (conocarpan and eupomathenoid-5) and the dichloromethane extract of Piper regnellii (Miq.) C. DC., Piperaceae, were tested against isolates of methicillin-resistant Staphylococcus aureus and methicillinsensitive S. aureus biofilms and S. aureus planktonic cells. The dichloromethane extract presented better results than isolated neolignans against all of the biofilms tested, with a minimum inhibitory concentration <400 g/ml for preformed biofilms and minimal biofilm inhibitory concentration of 15.6 g/ml for biofilm formation. The minimum inhibitory concentration to planktonic cells was <12.5 g/ml. These results indicate a good effect of the dichloromethane extract against methicillin-resistant S. aureus and methicillin-sensitive S. aureus biofilms and efficient prophylaxis.

Journal de mycologie médicale, 2015

Rosmarinus officinalis and Tetradenia riparia are used in folk medicine for the treatment of dise... more Rosmarinus officinalis and Tetradenia riparia are used in folk medicine for the treatment of disease, including infectious diseases and skin disorders. The purpose of this study was to investigate the antifungal activity of hydroalcoholic extracts from R. officinalis and T. riparia against strains of Trichophyton rubrum, T. mentagrophytes and Microsporum gypseum. Hydroalcoholic extracts prepared with dried leaves from R. officinalis, Psidium guajava and T. riparia were assayed against dermatophyte species by the microdilution technique and by microscopy. R. officinalis and T. riparia were the most active against dermatophytes, as determined from the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC), and were investigated further. Fluorescence microscopy and scanning electron microscopy were used to investigate inhibition of hyphal growth by the two extracts, and showed a strong inhibition and an irregular growth pattern. Both extracts showed good acti...

Journal of Applied Microbiology, 2017

This study aims to improve characteristics of Piper regnellii extract to make it applicable in fo... more This study aims to improve characteristics of Piper regnellii extract to make it applicable in formulations to treat dermatophytosis, also known as ringworm. Methods and results: Microparticles were produced by spray-drying with gelatin, alginate and chitosan as encapsulating agents, characterized by scanning electron microscopy, encapsulation efficiency, thermal analyses, X-ray diffraction, and tested against Trichophyton rubrum by broth microdilution. Produced microparticles had a mean diameter less than 2 µm, an increase in stability and release of the extract and good results for encapsulation Accepted Article This article is protected by copyright. All rights reserved. efficiency, being 85.6 % to gelatin microparticle, 71.3% to chitosan microparticle and 60.6 % to alginate. Microparticles preserved the antifungal activity of P. regnellii extract Trichophyton rubrum. Conclusion: Microencapsulation provided a significant improvement in the stability of the P. regnellii extract e better solubilization of chemical compounds, maintaining the antifungal effect against T. rubrum. Significance: These results are useful for developing a formulation to treat fungal infections caused by dermatophyte species.

Journal of Herbal Medicine

The therapeutic use of plant derivatives has increased in recent years, providing major advances ... more The therapeutic use of plant derivatives has increased in recent years, providing major advances in chemical and pharmacological aspects. In this study, aqueous and hydroalcoholic extracts of Eugenia uniflora, Piper diospyrifolium, Piper hispidum, Psidium guajava, Rosmarinus officinalis, Senna spectabilis and Tetradenia riparia were investigated for antimicrobial activity, employing the broth microdilution technique. The microorganisms tested were Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida parapsilosis and Candida tropicalis. All species showed some antimicrobial activity against bacteria and/or yeast species. S. aureus, B. subtilis and Candida species were most susceptible to the extracts. Five species were active against biofilm cells of C. albicans and S. aureus, and six species acted synergistically with fluconazole and/or nystatin against C. albicans, with FIC indexes less than 0.5. This study showed that these plant species could be useful sources for new antimicrobials.

The Journal of Prosthetic Dentistry, 2012

The level of passive fit on the interface structure/abutment may be affected by how Co-Cr prosthe... more The level of passive fit on the interface structure/abutment may be affected by how Co-Cr prosthetic superstructures are joined. However, despite their differences, the 3 joining methods are all capable of producing assemblies with a mean marginal misfit of less than 100 µm. Statement of problem. The influence of different joining techniques on passive fit at the interface structure/abutment of cobalt-chromium (Co-Cr) superstructures has not yet been clearly established.

Molecules, 2009

The chemical composition of the essential oil obtained from the leaves of Piper ovatum Vahl by hy... more The chemical composition of the essential oil obtained from the leaves of Piper ovatum Vahl by hydrodistillation was analyzed by GC-MS. The main constituents found were δ-amorphene (16.5 %), cis-muurola-4(14),5-diene (14.29 %) and γ-muurolene (13.26%). The crude extracts and isolated compounds were screened for their antimicrobial activity. Hydroalcoholic extracts of different parts of Piper ovatum Vahl, essential oil and amides isolated from leaves were tested against Gram-positive and Gram-negative bacteria and Candida species. All extracts and amides were active against Bacillus subtilis and Candida tropicalis, including clinical strains. Essential oil was active against C. tropicalis. These amides showed an inhibitory effect on the adherence of C. tropicalis ATCC 28707 on cover glasses at 10 µg/mL, but did not show morphological alterations at the tested concentrations. Amides were identified as piperovatine and piperlonguminine, and showed MIC values of 15.6 and 31.2 µg/mL to B. subtilis and 3.9 µg/mL to C. tropicalis, and low toxic effects to Vero cells and macrophages.