Elin Julianti - Academia.edu (original) (raw)
Papers by Elin Julianti
Acta Pharmaceutica Indonesia
ABSTRACTFungi are eukaryotic organisms that consist of unicellular organisms, namely molds and ye... more ABSTRACTFungi are eukaryotic organisms that consist of unicellular organisms, namely molds and yeasts, and multicellular organism known as mushrooms. In the medical field, fungi have a significant contribution as they are widely used as sources for discovering a lot of novel antibiotics. As the preliminary study, this paper presents the isolation of soil-derived fungi from Malaysian forest as resources for finding the new antibiotics and their test of antibacterial activity against Bacillus subtilis and Escherichia coli. The fungi their selves were isolated by using Sabouraud dextrose agar (SDA) medium. The pure fungi isolates were screened for their antibacterial activity by using disk diffusion method. The active fungi were fermented in Sabouraoud dextrose broth (SDB) medium for 21 days. Culture media and mycelium were separated by filtration method. The culture broth was extracted by liquid-liquid extraction and mycelium was extracted by maceration method using ethyl acetate. The...
Telah dilakukan penelitian pembentukan zat warna Monascus purpureus hasil fermentasi padat dengan... more Telah dilakukan penelitian pembentukan zat warna Monascus purpureus hasil fermentasi padat dengan dua variasi sampel limbah ampas kelapa sebagai substrat. Dilakukan pengukuran pembentukan zat warna dengan cara mengekstraksi sampel menggunakan etanol 95% pada fermentasi hari ke-7 dan ke-14 dilanjutkan dengan pengujian kromatografi lapis tipis menggunakan pengembang etanol:etilasetat (7:3). Serapan zat warna diukur dengan spektrofotometer UV-Vis pada panjang gelombang 400, 406, 498, 500, 511 dan 512 nm. Hasil menunjukkan serapan zat warna mengalami peningkatan dengan rentang serapan antara 0,128–0,269 dan 0,212-1,019 dan Sampel A menunjukkan serapan lebih tinggi dibandingkan Sampel B. Pengujian aktivitas antimikroba ekstrak zat warna ke dua sampel dilakukan terhadap Escherichia coli dan Candida albicans menunjukkan adanya aktivitas antimikroba. Kesetaraan aktivitas berdasarkan kurva baku Tetrasiklin HCl terhadap Escherichia coli dan kurva baku Nistatin terhadap Candida albicans menunj...
Journal of Mathematical and Fundamental Sciences, 2019
Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is wid... more Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freezethawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.
Biocatalysis and Agricultural Biotechnology, 2017
Microbiology Monographs, 2015
Traditional processed food in Asia is famous worldwide. For many years, some fermentative food-gr... more Traditional processed food in Asia is famous worldwide. For many years, some fermentative food-grade color pigments produced by microorganisms have been used to enhance their appearance. In Asia, mostly in China, India, Japan, and Indonesia, although natural colorants extracted from vegetables and fruits, such as saffron, turmeric, and suji leaf, have been widely used as food colorants, microbial color pigment is now intensely studied to produce a high-standard, high-yield, and stable food colorant, for example, more stable against light and heat, and with a wider range of pH than the natural food colorants. Some examples of the pigment molecules are carotenoids, melanin, flavin, quinones, monascins, and xanthophylls. Colorant-producing microorganisms, such as , , , and , can produce a large number of color pigments (Table 1). They grow mostly in soil and some of them grow on coast or in deep sea.
Marine Drugs, 2022
Colorectal cancer is one of the most common cancers diagnosed in the world. Chemotheraphy is one ... more Colorectal cancer is one of the most common cancers diagnosed in the world. Chemotheraphy is one of the most common methods used for the pharmacological treatment of this cancer patients. Nevertheless, the adverse effect of chemotherapy is not optimized for improving the quality of life of people who are older, who are the most vulnerable subpopulation. This review presents recent updates regarding secondary metabolites derived from marine fungi and actinobacteria as novel alternatives for cytotoxic agents against colorectal cancer cell lines HCT116, HT29, HCT15, RKO, Caco-2, and SW480. The observed marine-derived fungi were from the species Aspergillus sp., Penicillium sp., Neosartorya sp., Dichotomomyces sp., Paradendryphiella sp., and Westerdykella sp. Additionally, Streptomyces sp. and Nocardiopsis sp. are actinobacteria discussed in this study. Seventy one compounds reviewed in this study were grouped on the basis of their chemical structures. Indole alkaloids and diketopiperaz...
IOP Conference Series: Earth and Environmental Science
The search for new antibiotics from a variety of sources that have the potential to produce bioac... more The search for new antibiotics from a variety of sources that have the potential to produce bioactive metabolite compounds is essential to solve the increasing problem of microbial resistance. Recently, one of the most widely studied for new antimicrobial sources is marine derived fungi. The aims of this study were to isolate endophytic fungi of marine sponges collected from Sekotong Beach Lombok, Indonesia and determine their antimicrobial activity. Fungi isolation were conducted in yeast extract, peptone, and dextrose (YPD) agar medium and each pure fungi was fermented for 21 days in YPD broth medium. Culture media and mycelium were separated by filtration method. The culture media was extracted by liquid liquid extraction and mycelium was extracted by maceration method using ethyl acetate. The dried extracts then tested for their antibacterial activity against Escherichia coli ATCC8739, Staphylococcus aureus ATCC6538, and Candida albicans ATCC 10231 using paper disk diffusion method. Extracts which showed antimicrobial activities on 250 ug/disk content were determined their minimum inhibitory concentration (MIC) and minimum bactericidal or Fungicidal concentration (MBC or MFC). The results of this research were obtained 12 fungi isolates that were coded as FSKT1 until FSKT12. The lowest MIC and MBC value against E. coli was showed by mycelium extract of FSKT10 with concentration of 128 and 256 ug/mL, respectively. The lowest MIC and MBC value against S. aureus was showed by medium extract of FSKT9 with concentration of 64 ug/mL and against C. albicans with concentration of 128 and 512 ug/mL, respectively. The antimicrobial activity observed in this research indicates that the endophytyc fungi of the marine sponge could be considered as new sources of antibiotics.
Marine Research in Indonesia
Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowaday... more Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA wer...
Tetrahedron Letters, 2012
ABSTRACT
Tetrahedron Letters, 2013
ABSTRACT
Phytochemistry, 2012
Ten sesquiterpenes, together with 12 known compounds were isolated from leaves of Laurus nobilis ... more Ten sesquiterpenes, together with 12 known compounds were isolated from leaves of Laurus nobilis L. Based on spectroscopic analyses, the 10 compounds were determined to be eudesmane lactones and their corresponding methyl esters. Most of these compounds exhibited moderate-to-significant cytotoxicity towards K562 leukemia cells. One compound had a higher cytotoxicity than doxorubicin, while other compounds had moderate to no activity.
Organic Letters, 2013
Herqueiazole (1), herqueioxazole (2), and herqueidiketal (3), polyaromatic metabolites with a nov... more Herqueiazole (1), herqueioxazole (2), and herqueidiketal (3), polyaromatic metabolites with a novel skeletal class, were isolated from the marine-derived fungus Penicillium sp. Based on the combined spectroscopic analyses, the structures of 1 and 2 were determined to be the first examples of pyrrole- and oxazole-containing phenalenone compounds, respectively, whereas 3 possessed a novel skeleton with a highly oxidized naphthoquinone moiety. Compound 3 exhibited moderate cytotoxicity and significant inhibitory activity against sortase A.
Journal of Natural Products, 2011
The novel natural product acremostrictin was isolated from the culture broth of Acremonium strict... more The novel natural product acremostrictin was isolated from the culture broth of Acremonium strictum, a marine fungus collected from a Choristida sponge off the coast of Korea. Structurally, acremostrictin is a tricyclic lactone of an unprecedented skeletal class based on combined spectroscopic and X-ray crystallographic analyses. The new compound exhibited weak antibacterial and moderate antioxidant activities.
Acta Pharmaceutica Indonesia
ABSTRACTFungi are eukaryotic organisms that consist of unicellular organisms, namely molds and ye... more ABSTRACTFungi are eukaryotic organisms that consist of unicellular organisms, namely molds and yeasts, and multicellular organism known as mushrooms. In the medical field, fungi have a significant contribution as they are widely used as sources for discovering a lot of novel antibiotics. As the preliminary study, this paper presents the isolation of soil-derived fungi from Malaysian forest as resources for finding the new antibiotics and their test of antibacterial activity against Bacillus subtilis and Escherichia coli. The fungi their selves were isolated by using Sabouraud dextrose agar (SDA) medium. The pure fungi isolates were screened for their antibacterial activity by using disk diffusion method. The active fungi were fermented in Sabouraoud dextrose broth (SDB) medium for 21 days. Culture media and mycelium were separated by filtration method. The culture broth was extracted by liquid-liquid extraction and mycelium was extracted by maceration method using ethyl acetate. The...
Telah dilakukan penelitian pembentukan zat warna Monascus purpureus hasil fermentasi padat dengan... more Telah dilakukan penelitian pembentukan zat warna Monascus purpureus hasil fermentasi padat dengan dua variasi sampel limbah ampas kelapa sebagai substrat. Dilakukan pengukuran pembentukan zat warna dengan cara mengekstraksi sampel menggunakan etanol 95% pada fermentasi hari ke-7 dan ke-14 dilanjutkan dengan pengujian kromatografi lapis tipis menggunakan pengembang etanol:etilasetat (7:3). Serapan zat warna diukur dengan spektrofotometer UV-Vis pada panjang gelombang 400, 406, 498, 500, 511 dan 512 nm. Hasil menunjukkan serapan zat warna mengalami peningkatan dengan rentang serapan antara 0,128–0,269 dan 0,212-1,019 dan Sampel A menunjukkan serapan lebih tinggi dibandingkan Sampel B. Pengujian aktivitas antimikroba ekstrak zat warna ke dua sampel dilakukan terhadap Escherichia coli dan Candida albicans menunjukkan adanya aktivitas antimikroba. Kesetaraan aktivitas berdasarkan kurva baku Tetrasiklin HCl terhadap Escherichia coli dan kurva baku Nistatin terhadap Candida albicans menunj...
Journal of Mathematical and Fundamental Sciences, 2019
Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is wid... more Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freezethawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.
Biocatalysis and Agricultural Biotechnology, 2017
Microbiology Monographs, 2015
Traditional processed food in Asia is famous worldwide. For many years, some fermentative food-gr... more Traditional processed food in Asia is famous worldwide. For many years, some fermentative food-grade color pigments produced by microorganisms have been used to enhance their appearance. In Asia, mostly in China, India, Japan, and Indonesia, although natural colorants extracted from vegetables and fruits, such as saffron, turmeric, and suji leaf, have been widely used as food colorants, microbial color pigment is now intensely studied to produce a high-standard, high-yield, and stable food colorant, for example, more stable against light and heat, and with a wider range of pH than the natural food colorants. Some examples of the pigment molecules are carotenoids, melanin, flavin, quinones, monascins, and xanthophylls. Colorant-producing microorganisms, such as , , , and , can produce a large number of color pigments (Table 1). They grow mostly in soil and some of them grow on coast or in deep sea.
Marine Drugs, 2022
Colorectal cancer is one of the most common cancers diagnosed in the world. Chemotheraphy is one ... more Colorectal cancer is one of the most common cancers diagnosed in the world. Chemotheraphy is one of the most common methods used for the pharmacological treatment of this cancer patients. Nevertheless, the adverse effect of chemotherapy is not optimized for improving the quality of life of people who are older, who are the most vulnerable subpopulation. This review presents recent updates regarding secondary metabolites derived from marine fungi and actinobacteria as novel alternatives for cytotoxic agents against colorectal cancer cell lines HCT116, HT29, HCT15, RKO, Caco-2, and SW480. The observed marine-derived fungi were from the species Aspergillus sp., Penicillium sp., Neosartorya sp., Dichotomomyces sp., Paradendryphiella sp., and Westerdykella sp. Additionally, Streptomyces sp. and Nocardiopsis sp. are actinobacteria discussed in this study. Seventy one compounds reviewed in this study were grouped on the basis of their chemical structures. Indole alkaloids and diketopiperaz...
IOP Conference Series: Earth and Environmental Science
The search for new antibiotics from a variety of sources that have the potential to produce bioac... more The search for new antibiotics from a variety of sources that have the potential to produce bioactive metabolite compounds is essential to solve the increasing problem of microbial resistance. Recently, one of the most widely studied for new antimicrobial sources is marine derived fungi. The aims of this study were to isolate endophytic fungi of marine sponges collected from Sekotong Beach Lombok, Indonesia and determine their antimicrobial activity. Fungi isolation were conducted in yeast extract, peptone, and dextrose (YPD) agar medium and each pure fungi was fermented for 21 days in YPD broth medium. Culture media and mycelium were separated by filtration method. The culture media was extracted by liquid liquid extraction and mycelium was extracted by maceration method using ethyl acetate. The dried extracts then tested for their antibacterial activity against Escherichia coli ATCC8739, Staphylococcus aureus ATCC6538, and Candida albicans ATCC 10231 using paper disk diffusion method. Extracts which showed antimicrobial activities on 250 ug/disk content were determined their minimum inhibitory concentration (MIC) and minimum bactericidal or Fungicidal concentration (MBC or MFC). The results of this research were obtained 12 fungi isolates that were coded as FSKT1 until FSKT12. The lowest MIC and MBC value against E. coli was showed by mycelium extract of FSKT10 with concentration of 128 and 256 ug/mL, respectively. The lowest MIC and MBC value against S. aureus was showed by medium extract of FSKT9 with concentration of 64 ug/mL and against C. albicans with concentration of 128 and 512 ug/mL, respectively. The antimicrobial activity observed in this research indicates that the endophytyc fungi of the marine sponge could be considered as new sources of antibiotics.
Marine Research in Indonesia
Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowaday... more Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA wer...
Tetrahedron Letters, 2012
ABSTRACT
Tetrahedron Letters, 2013
ABSTRACT
Phytochemistry, 2012
Ten sesquiterpenes, together with 12 known compounds were isolated from leaves of Laurus nobilis ... more Ten sesquiterpenes, together with 12 known compounds were isolated from leaves of Laurus nobilis L. Based on spectroscopic analyses, the 10 compounds were determined to be eudesmane lactones and their corresponding methyl esters. Most of these compounds exhibited moderate-to-significant cytotoxicity towards K562 leukemia cells. One compound had a higher cytotoxicity than doxorubicin, while other compounds had moderate to no activity.
Organic Letters, 2013
Herqueiazole (1), herqueioxazole (2), and herqueidiketal (3), polyaromatic metabolites with a nov... more Herqueiazole (1), herqueioxazole (2), and herqueidiketal (3), polyaromatic metabolites with a novel skeletal class, were isolated from the marine-derived fungus Penicillium sp. Based on the combined spectroscopic analyses, the structures of 1 and 2 were determined to be the first examples of pyrrole- and oxazole-containing phenalenone compounds, respectively, whereas 3 possessed a novel skeleton with a highly oxidized naphthoquinone moiety. Compound 3 exhibited moderate cytotoxicity and significant inhibitory activity against sortase A.
Journal of Natural Products, 2011
The novel natural product acremostrictin was isolated from the culture broth of Acremonium strict... more The novel natural product acremostrictin was isolated from the culture broth of Acremonium strictum, a marine fungus collected from a Choristida sponge off the coast of Korea. Structurally, acremostrictin is a tricyclic lactone of an unprecedented skeletal class based on combined spectroscopic and X-ray crystallographic analyses. The new compound exhibited weak antibacterial and moderate antioxidant activities.