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Papers by Eloka Chidiebere

This study evaluates the rate of bone decalcification at varying temperatures, decalcification fl... more This study evaluates the rate of bone decalcification at varying temperatures, decalcification fluid and fluid
concentrations. A decalcification methodology was adopted using modern household microwave oven to accelerate the decalcification rate of rabbit compact bone sample. Bone biopsy was obtained from a rabbit limb and fixed immediately for 24hrs in 10% formalin. Traditional decalcification was carried out at room temperature (RT) with
5% Jenkins fluid (JK) and 5% Gooding and Stewart (GS) fluid as controls. Microwave oven decalcification with 5% JK and 7% JK, as well as 5% GS and 10% GS served as the tests at 300C, 400C and 500C in microwave oven. Results
showed that at 300C, 5%-GS and 10%-GS as well as 5%-JK and 7%-JK presented a decalcifying time of
11hrs:30mins and 10hrs:30mins as well as 15hrs and 13hrs respectively. At 400C, a decalcifying time of 8hrs,
6hrs:30mins, 10hrs:30mins and 12hrs were recorded for the respective concentrations of decalcifying fluid. Also, at 50C, the decalcifying times were 6hrs, 5hrs, and 9hrs:30mins and 8hrs respectively. The observed differences between RT and microwave oven decalcification was significant while the rate between the two decalcifying fluid were not. Our findings further encourage the use of microwave oven for bone decalcification.

This study was designed to determine the prevalence of soil-transmitted helminthes infections amo... more This study was designed to determine the prevalence of soil-transmitted helminthes infections among primary school pupils in Ekpoma, Edo State Nigeria. Stool samples were collected from 200 apparently healthy children between November 2011 and April 2012. The samples were examined for parasitic infections using direct smear examination and formol ether concentration techniques. Results showed an overall prevalence of 24.50% soiltransmitted helminthes infections amongst the sampled population. The various soil-transmited helminths isolated were Ascaris lumbricoides (4%) Hookworm (15%), Trichuris trichuria (0.5%) and strongyloides stercolalis (1%) respectively. Also, 4.0% of the pupils were infected by more than one species of soil-transmitted helminths. The infection was higher among the males (28%), and pupils of age group 6-10 (27.30%). Hookworm infection had the highest prevalence compared to other isolates but the associated differences in age and sex among the infected children were statistically, not significant (p > 0.05). Therefore, the relative high prevalence and diversity of soil transmitted helminthes amongst children in Ekpoma, underlines the urgent need for intervening control measures in the area. Various control measures are suggested and our findings are further discussed.

In an eight-week study on the effect of potash-tobacco dust ingestion on rats, a persistent penil... more In an eight-week study on the effect of potash-tobacco dust ingestion on rats, a persistent penile erection for days was observed amongst some of the rats under study. This study involved a total of 42 Wister rats of weights ranging between 150-300g and grouped into four groups (A, B, C and D). Group A served as control, while groups B -D served as test groups and received 2g, 4g, and 6g of tobacco-dust, as well as 0.4g, 0.8g, and 1.2g of potash respectively, with water given ad libitum. By the 4 th week, a persistent penile erection was observed in one of the rats in group C, and by the 6 th week, a similar incident occurred in group C and D respectively, prompting serial observation. By the 8 week, gangrenous changes were observed, followed by necrosis and death of the rats on the 10 th day from onset. The control rats (group A), which received normal feed and water, remained normal.

This study was designed to evaluate the effect of decalcifying fluid types on bone tissue archite... more This study was designed to evaluate the effect of decalcifying fluid types on bone tissue architecture and its staining properties following decalcification at varying temperatures. A decalcification methodology using Golding and Stewards (GS) fluid, and Jenkings fluid (JK), and a modern household microwave oven to accelerate the decalcification rate of bone tissue samples, was adopted for microscopic investigations at room temperature, 30 0 C, 40 0 C, and 50 0 C respectively. Bone biopsy was obtained from a rabbit's limb and fixed immediately for 24hrs in 10% formalin. The result showed that micrographs from bone tissue sections decalcified with 5% GS presented excellent histological architecture when compared with those decalcified with 5% JK at 30 0 C while 5% JK presented good histological architecture when compared with those decalcified with 5% GS at 40 0 C.Severe cytoarchitectural distortion was observed for bone tissues decalcified with 5% GS at 50 0 C, while bone tissues decalcified with 5% JK at 50 0 C, presented cyto-architectural distortions that were however, not as severe as those of 5% GS at 50 0 C. Our findings suggest therefore, that decalcification with 5% GS at 30 0 C, comparatively gives optimal histological results and preserves the tissues' best staining properties.