Emil Trofimiuk - Academia.edu (original) (raw)
Papers by Emil Trofimiuk
Acta Neurobiologiae Experimentalis, 2005
It is recognized that chronic stress is an important risk factor for the development of several c... more It is recognized that chronic stress is an important risk factor for the development of several cognitive impairments involving working memory. Working memory refers to the memory in which the information to be remembered changes from trial-to-trial and should be assessed in a task able to detect retrieval of that information. In the present study, we tested the hypothesis that preventive administration of Hypericum perforatum (also named St John's wort) may counteract the working memory impairments caused by repeated stress. Specifically, we attempted to characterize the preventive action of longlasting treatment with St John's wort (350 mg/kg, p.o.) on the spatial working memory impairments caused by chronic restraint stress (2 h daily for 21 days) or durable medication with exogenous corticosterone (5 mg/kg, s.c.) in male Wistar rats. Spatial working memory was tested in Barnes maze (BM) and in the Morris water maze (MWM). We found that H. perforatum prevented the deleterious effects of both chronic restraint stress and prolonged corticosterone on working memory measured in both tests. The herb significantly improved hippocampus dependent spatial working memory in comparison with control (p<0.01) and alleviated some other negative effects of stress on cognitive functions.
Acta Neurobiologiae Experimentalis, 2011
Current Topics in Nutraceutical Research, 2010
Minimal anti-tobacco intervention (MIA) is an efficient and inexpensive method of smoking cessati... more Minimal anti-tobacco intervention (MIA) is an efficient and inexpensive method of smoking cessation intervention. The ability for practical use of MIA could be acquired by a majority of physicians-practitioners in Poland and other European Union countries. The objective of the work was to establish a practical knowledge in the scope of anti-tobacco strategies of senior students of the Medical Faculty of the Medical University of Bialystok after six-year education. We also assessed the prevalence of tobacco smoking among students of Medical Faculty. The percentage of tobacco smokers between the last course students of Medical Faculty average at 15% of women and 29% of men. Only 3 of all woman and half of men think they could effectively help in smoking cessation, but most of the assessed students have not acquired sufficient knowledge for an anti-tobacco intervention as well as they don't know practical tools like Fagerstöm Test for Nicotine Dependence or Schneider's Smoker C...
Acta Neurobiologiae Experimentalis, 2005
Przeglad lekarski, 2005
According to the guidelines of the National Health Program, assumed by the Polish Ministry of Hea... more According to the guidelines of the National Health Program, assumed by the Polish Ministry of Health for the years 1996 - 2005, it could be expected that, particularly at medical universities, education of future doctors in the field of problems connected to addiction to nicotine is taken into consideration. However, the research conducted in the form of anonymous survey among the sixth year students of the Faculty of Medicine at the Medical University of Białystok in the academic years 2001/2002 and 2004/2005 does not corroborate those expectations. The survey proves that the future graduates' knowledge of the aforementioned topic is fragmentary and confirms the necessity of including that issue in the educational curriculum in the area of family medicine.
Respiratory diseases are the most common cause of the child and family practice physicians are on... more Respiratory diseases are the most common cause of the child and family practice physicians are one of the main reasons for referral to a specialist clinic and hospital pediatric wards. The severity of respiratory disease in adolescence influenced by various factors, endo- and exogenous. Some of them, especially environmental factors can be eliminated or reduced and thus reduce the risk of developing this disease. The most common source of pollutants in dwellings is tobacco smoke. The aim of this study was to assess exposure to tobacco smoke in three years old children of attending local kindergartens in Białystok and its influence on the type of recovery from acute respiratory infections by the respondents. The study included 313 children from among the 1,200 who attend the local 51-kindergartens in Bialystok. Information on the structure of tobacco use in three-years-old-children's families and respiratory illnesses among random children were obtained, based on anonymous questi...
Exposure to chronic restraint stress in rats and psychosocial stress in humans has been shown to ... more Exposure to chronic restraint stress in rats and psychosocial stress in humans has been shown to alter cognitive functions such as learning and memory and has been linked to the pathophysiology of mood and anxiety disorders. Antianxiety or sedative agents used in the management of stress have several disadvantages and undesired effects. Therefore, in this study, we investigated efficacy of a natural medicine, the extract of Ginkgo biloba (EGB 761), in prevention and treatment of the post-stress memory dysfunctions. The results showed that chronic restraint stress (2 h for 21 days) or an 'equivalent' dose of exogenous corticosterone (5 mg/kg) impaired nonspatial memory as measured by an object recognition test. In control rats, EGB 761 improved spatial and nonspatial memory in Morris water maze and object recognition tests. Preventive doses of EGB 761 (100 mg/kg) normalized cognitive deficits, seen in rats chronically stressed or treated with corticosterone in object recognit...
In the years 1998-2005 in Poland a National Health Program was being implemented. One of its chie... more In the years 1998-2005 in Poland a National Health Program was being implemented. One of its chief guidelines was to reduce the popularity of tobacco smoking, recognised as a proven, single factor influencing the etiology and the course of many diseases. The objective of the work was to establish whether the program's guidelines were reflected in individual attitudes of senior students of the Faculty of Medicine and of the Division of Public Health of Medical University of Bialystok. The research was conducted with the use of an anonymous questionnaire, filled in by the students before the classes dedicated to the issues of nicotine addiction within the framework of the family medicine thematic block. The analysis of the results did not show a decrease in the number of student smokers in comparison to the previous years; however, it was observed that more than a half of the current smokers had tried and was going to try to give up smoking, and the non-smokers strongly objected t...
Experimental Lung Research
ABSTRACT Purpose: Cellular response to cigarette smoke (CS) involves activation of recognition re... more ABSTRACT Purpose: Cellular response to cigarette smoke (CS) involves activation of recognition receptors resulting in changes in immune status, oxidative stress and cell turnover. We investigated the effects of CS on sialic acid-binding immunoglobulin type lectins (Siglecs) expression and their sialylated ligands in human immune and non-immune cells. Methods: Human monocytes (THP-1) and epithelial cells (A549) were cultured in CS–conditioned medium (CSM). Expression of Siglec-8 and Siglec-5/Siglec-14 was analysed in THP-1 cells using flow cytometry. The effects of CS on immune activity was evaluated flow cytometrically in these cells by assessment of phagocytosis and intracellular expression IL-1β and IL-10. Detection and differentiation of sialic acids was analyzed by dot blot, western blot and flow cytometry using plant lectins and antibodies. Results: Exposure to CS significantly increased expression of Siglec-8 and Siglec-5/Siglec-14 in THP-1 cells. These changes were accompanied by enhanced intracellular level of IL-1β and IL-10 but reduced phagocytic activity. In THP-1 and A549 cells, the level of α2,3-sialic acids, but not α2,6-sialic acid, was significantly increased when compared to naïve cells. The level of α2,8-sialic acids increased significantly in A549 cells, but not in THP-1 cells, after exposure to CS. Conclusion: These results show that cellular response to CS involves changes in expression of Siglec receptors and sialylated ligands functionally associated with immunity.
Acta Haematologica, 2004
Acute lymphoblastic leukemia 111-myelogenous leukemia 164-nonlymphocytic leukemia 160 Allele-spec... more Acute lymphoblastic leukemia 111-myelogenous leukemia 164-nonlymphocytic leukemia 160 Allele-specific oligonucleotide polymerase chain reaction, multiple myeloma 105 Amifostine 136 Anemia 126
Acta Haematologica, 2004
Access to full text and tables of contents, including tentative ones for forthcoming issues:
Frontiers in Systems Neuroscience
Background: A considerable number of competitive antagonists/inverse agonists of histamine H3 rec... more Background: A considerable number of competitive antagonists/inverse agonists of histamine H3 receptor (H3R) have progressed to clinical assessment, with pitolisant approved for the treatment of narcolepsy. H3R, highly expressed in the CNS, is regarded as a relevant target in CNS disorders. At the same time, new compounds including ABT-239 H3R antagonist (ABT; benzonitrile, 4-[2-[2-[(2R)-2-methyl-1-pyrrolidinyl]ethyl]-5-benzofuranyl]-) are continually being tested. The study aimed to test ABT-239 as a prophylactic agent in stress-induced memory impairments.Methods: Stressed and non-stressed rats were pre-treated with ABT-239 and subsequently subjected to several behavioral tests aimed at assessing the animals’ working and spatial reference memory [Morris water maze (MWM), Barnes maze (BM)], assessing the locomotor function and anxiety-like behavior [Open field (OF), elevated “plus” maze—EPM].Results: Chronically stressed rats displayed a significant decline in spatial (working and r...
Behavioural Brain Research
Molecular and Cellular Biochemistry
, are commonly used to control tumor-induced edema in the brain tumor patients. There are increas... more , are commonly used to control tumor-induced edema in the brain tumor patients. There are increasing evidences that immunosuppressive action of Dex interferes with immune surveillance resulting in lower patients overall survival; however, the mechanisms underlying these actions remain unclear. Changes in the expression of sialic acids are critical features of many cancers that reduce their immunogenicity and increase viability. Sialoglycans can be recognized by CD33-related Siglecs that negatively regulate the immune response and thereby impair immune surveillance. In this study, we analysed the effect of Dex on cell surface sialylation pattern and recognition of these structures by Siglec-F receptor in poorly immunogenic GL261 and immunogenic SMA560 glioma cells. Relative amount of α2.3-, α2.6-and α2.8-linked sialic acids were detected by Western blot with MAA (Maackia amurensis) and SNA (Sambucus nigra) lectins, and flow cytometry using monoclonal antibody anti-PSA-NCAM. In response to Dex, α2.8 sialylation in both, GL261 and SMA560 was increased, whereas the level of α2.3-linked sialic acids remained unchanged. Moreover, we found the opposite effects of Dex on α2.6 sialylation in poorly immunogenic and immunogenic glioma cells. Furthermore, changes in sialylation pattern were accompanied by dose-dependent effects of Dex on Siglec-F binding to glioma cell membranes as well as decreased α-neuraminidase activity. These results suggest that glucocorticosteroid-induced alterations in cell surface sialylation and Siglecs recognition may dampen anti-tumor immunity, and participate in glioma-promoting process by immune cells. Our study gives new view on corticosteroid therapy in glioma patients.
Canadian Institute of Food Science and Technology Journal
Acta Neurobiologiae Experimentalis, 2005
It is recognized that chronic stress is an important risk factor for the development of several c... more It is recognized that chronic stress is an important risk factor for the development of several cognitive impairments involving working memory. Working memory refers to the memory in which the information to be remembered changes from trial-to-trial and should be assessed in a task able to detect retrieval of that information. In the present study, we tested the hypothesis that preventive administration of Hypericum perforatum (also named St John's wort) may counteract the working memory impairments caused by repeated stress. Specifically, we attempted to characterize the preventive action of longlasting treatment with St John's wort (350 mg/kg, p.o.) on the spatial working memory impairments caused by chronic restraint stress (2 h daily for 21 days) or durable medication with exogenous corticosterone (5 mg/kg, s.c.) in male Wistar rats. Spatial working memory was tested in Barnes maze (BM) and in the Morris water maze (MWM). We found that H. perforatum prevented the deleterious effects of both chronic restraint stress and prolonged corticosterone on working memory measured in both tests. The herb significantly improved hippocampus dependent spatial working memory in comparison with control (p<0.01) and alleviated some other negative effects of stress on cognitive functions.
Acta Neurobiologiae Experimentalis, 2011
Current Topics in Nutraceutical Research, 2010
Minimal anti-tobacco intervention (MIA) is an efficient and inexpensive method of smoking cessati... more Minimal anti-tobacco intervention (MIA) is an efficient and inexpensive method of smoking cessation intervention. The ability for practical use of MIA could be acquired by a majority of physicians-practitioners in Poland and other European Union countries. The objective of the work was to establish a practical knowledge in the scope of anti-tobacco strategies of senior students of the Medical Faculty of the Medical University of Bialystok after six-year education. We also assessed the prevalence of tobacco smoking among students of Medical Faculty. The percentage of tobacco smokers between the last course students of Medical Faculty average at 15% of women and 29% of men. Only 3 of all woman and half of men think they could effectively help in smoking cessation, but most of the assessed students have not acquired sufficient knowledge for an anti-tobacco intervention as well as they don't know practical tools like Fagerstöm Test for Nicotine Dependence or Schneider's Smoker C...
Acta Neurobiologiae Experimentalis, 2005
Przeglad lekarski, 2005
According to the guidelines of the National Health Program, assumed by the Polish Ministry of Hea... more According to the guidelines of the National Health Program, assumed by the Polish Ministry of Health for the years 1996 - 2005, it could be expected that, particularly at medical universities, education of future doctors in the field of problems connected to addiction to nicotine is taken into consideration. However, the research conducted in the form of anonymous survey among the sixth year students of the Faculty of Medicine at the Medical University of Białystok in the academic years 2001/2002 and 2004/2005 does not corroborate those expectations. The survey proves that the future graduates' knowledge of the aforementioned topic is fragmentary and confirms the necessity of including that issue in the educational curriculum in the area of family medicine.
Respiratory diseases are the most common cause of the child and family practice physicians are on... more Respiratory diseases are the most common cause of the child and family practice physicians are one of the main reasons for referral to a specialist clinic and hospital pediatric wards. The severity of respiratory disease in adolescence influenced by various factors, endo- and exogenous. Some of them, especially environmental factors can be eliminated or reduced and thus reduce the risk of developing this disease. The most common source of pollutants in dwellings is tobacco smoke. The aim of this study was to assess exposure to tobacco smoke in three years old children of attending local kindergartens in Białystok and its influence on the type of recovery from acute respiratory infections by the respondents. The study included 313 children from among the 1,200 who attend the local 51-kindergartens in Bialystok. Information on the structure of tobacco use in three-years-old-children's families and respiratory illnesses among random children were obtained, based on anonymous questi...
Exposure to chronic restraint stress in rats and psychosocial stress in humans has been shown to ... more Exposure to chronic restraint stress in rats and psychosocial stress in humans has been shown to alter cognitive functions such as learning and memory and has been linked to the pathophysiology of mood and anxiety disorders. Antianxiety or sedative agents used in the management of stress have several disadvantages and undesired effects. Therefore, in this study, we investigated efficacy of a natural medicine, the extract of Ginkgo biloba (EGB 761), in prevention and treatment of the post-stress memory dysfunctions. The results showed that chronic restraint stress (2 h for 21 days) or an 'equivalent' dose of exogenous corticosterone (5 mg/kg) impaired nonspatial memory as measured by an object recognition test. In control rats, EGB 761 improved spatial and nonspatial memory in Morris water maze and object recognition tests. Preventive doses of EGB 761 (100 mg/kg) normalized cognitive deficits, seen in rats chronically stressed or treated with corticosterone in object recognit...
In the years 1998-2005 in Poland a National Health Program was being implemented. One of its chie... more In the years 1998-2005 in Poland a National Health Program was being implemented. One of its chief guidelines was to reduce the popularity of tobacco smoking, recognised as a proven, single factor influencing the etiology and the course of many diseases. The objective of the work was to establish whether the program's guidelines were reflected in individual attitudes of senior students of the Faculty of Medicine and of the Division of Public Health of Medical University of Bialystok. The research was conducted with the use of an anonymous questionnaire, filled in by the students before the classes dedicated to the issues of nicotine addiction within the framework of the family medicine thematic block. The analysis of the results did not show a decrease in the number of student smokers in comparison to the previous years; however, it was observed that more than a half of the current smokers had tried and was going to try to give up smoking, and the non-smokers strongly objected t...
Experimental Lung Research
ABSTRACT Purpose: Cellular response to cigarette smoke (CS) involves activation of recognition re... more ABSTRACT Purpose: Cellular response to cigarette smoke (CS) involves activation of recognition receptors resulting in changes in immune status, oxidative stress and cell turnover. We investigated the effects of CS on sialic acid-binding immunoglobulin type lectins (Siglecs) expression and their sialylated ligands in human immune and non-immune cells. Methods: Human monocytes (THP-1) and epithelial cells (A549) were cultured in CS–conditioned medium (CSM). Expression of Siglec-8 and Siglec-5/Siglec-14 was analysed in THP-1 cells using flow cytometry. The effects of CS on immune activity was evaluated flow cytometrically in these cells by assessment of phagocytosis and intracellular expression IL-1β and IL-10. Detection and differentiation of sialic acids was analyzed by dot blot, western blot and flow cytometry using plant lectins and antibodies. Results: Exposure to CS significantly increased expression of Siglec-8 and Siglec-5/Siglec-14 in THP-1 cells. These changes were accompanied by enhanced intracellular level of IL-1β and IL-10 but reduced phagocytic activity. In THP-1 and A549 cells, the level of α2,3-sialic acids, but not α2,6-sialic acid, was significantly increased when compared to naïve cells. The level of α2,8-sialic acids increased significantly in A549 cells, but not in THP-1 cells, after exposure to CS. Conclusion: These results show that cellular response to CS involves changes in expression of Siglec receptors and sialylated ligands functionally associated with immunity.
Acta Haematologica, 2004
Acute lymphoblastic leukemia 111-myelogenous leukemia 164-nonlymphocytic leukemia 160 Allele-spec... more Acute lymphoblastic leukemia 111-myelogenous leukemia 164-nonlymphocytic leukemia 160 Allele-specific oligonucleotide polymerase chain reaction, multiple myeloma 105 Amifostine 136 Anemia 126
Acta Haematologica, 2004
Access to full text and tables of contents, including tentative ones for forthcoming issues:
Frontiers in Systems Neuroscience
Background: A considerable number of competitive antagonists/inverse agonists of histamine H3 rec... more Background: A considerable number of competitive antagonists/inverse agonists of histamine H3 receptor (H3R) have progressed to clinical assessment, with pitolisant approved for the treatment of narcolepsy. H3R, highly expressed in the CNS, is regarded as a relevant target in CNS disorders. At the same time, new compounds including ABT-239 H3R antagonist (ABT; benzonitrile, 4-[2-[2-[(2R)-2-methyl-1-pyrrolidinyl]ethyl]-5-benzofuranyl]-) are continually being tested. The study aimed to test ABT-239 as a prophylactic agent in stress-induced memory impairments.Methods: Stressed and non-stressed rats were pre-treated with ABT-239 and subsequently subjected to several behavioral tests aimed at assessing the animals’ working and spatial reference memory [Morris water maze (MWM), Barnes maze (BM)], assessing the locomotor function and anxiety-like behavior [Open field (OF), elevated “plus” maze—EPM].Results: Chronically stressed rats displayed a significant decline in spatial (working and r...
Behavioural Brain Research
Molecular and Cellular Biochemistry
, are commonly used to control tumor-induced edema in the brain tumor patients. There are increas... more , are commonly used to control tumor-induced edema in the brain tumor patients. There are increasing evidences that immunosuppressive action of Dex interferes with immune surveillance resulting in lower patients overall survival; however, the mechanisms underlying these actions remain unclear. Changes in the expression of sialic acids are critical features of many cancers that reduce their immunogenicity and increase viability. Sialoglycans can be recognized by CD33-related Siglecs that negatively regulate the immune response and thereby impair immune surveillance. In this study, we analysed the effect of Dex on cell surface sialylation pattern and recognition of these structures by Siglec-F receptor in poorly immunogenic GL261 and immunogenic SMA560 glioma cells. Relative amount of α2.3-, α2.6-and α2.8-linked sialic acids were detected by Western blot with MAA (Maackia amurensis) and SNA (Sambucus nigra) lectins, and flow cytometry using monoclonal antibody anti-PSA-NCAM. In response to Dex, α2.8 sialylation in both, GL261 and SMA560 was increased, whereas the level of α2.3-linked sialic acids remained unchanged. Moreover, we found the opposite effects of Dex on α2.6 sialylation in poorly immunogenic and immunogenic glioma cells. Furthermore, changes in sialylation pattern were accompanied by dose-dependent effects of Dex on Siglec-F binding to glioma cell membranes as well as decreased α-neuraminidase activity. These results suggest that glucocorticosteroid-induced alterations in cell surface sialylation and Siglecs recognition may dampen anti-tumor immunity, and participate in glioma-promoting process by immune cells. Our study gives new view on corticosteroid therapy in glioma patients.
Canadian Institute of Food Science and Technology Journal