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Papers by Endrigo Pompermayer

Research paper thumbnail of Anticorpos anti-Neospora spp. em amostras sorológicas de potros pré-colostrais pela técnica de imunofluorescência indireta

Ciência Rural, 2012

ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora s... more ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora spp. in samples of presuckle foal, as weel as determine the best dilution of serum to be used in indirect fluorescent antibody test. We analyzed serum samples from 203 presuckle foals, by indirect fluorescent antibody test in different titrations. The titrations of 16 and 50 showed 25.1% and 9.9% of presuckle foals positive, respectively. Thus, in serum samples from presuckle foals the titration 16 can be considered more appropriate to detect the occurrence of infection by the protozoan and therefore the transplacental transmission of Neospora spp. in horses.

Research paper thumbnail of Anticorpos anti-Neospora spp. em amostras sorológicas de potros pré-colostrais pela técnica de imunofluorescência indireta

Ciência Rural, 2012

ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora s... more ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora spp. in samples of presuckle foal, as weel as determine the best dilution of serum to be used in indirect fluorescent antibody test. We analyzed serum samples from 203 presuckle foals, by indirect fluorescent antibody test in different titrations. The titrations of 16 and 50 showed 25.1% and 9.9% of presuckle foals positive, respectively. Thus, in serum samples from presuckle foals the titration 16 can be considered more appropriate to detect the occurrence of infection by the protozoan and therefore the transplacental transmission of Neospora spp. in horses.

Research paper thumbnail of Claudicação em cavalos Crioulos atletas

Ciência Rural, 2011

O objetivo deste trabalho foi identificar as principais causas de claudicação em cavalos Crioulos... more O objetivo deste trabalho foi identificar as principais causas de claudicação em cavalos Crioulos em treinamento para a prova do Freio de Ouro e competições de rédeas. Foram avaliados os registros clínicos de 201 cavalos Crioulos, 5,62,1 anos de idade, submetidos a exame físico devido à presença de claudicação ou histórico de baixo rendimento atlético entre os anos de 2002 a 2009. A claudicação foi localizada nos membros torácicos e pélvicos em 47,1% (n=105) e 52,9% (n=118), respectivamente. Nos machos, houve uma predominância de claudicação nos membros pélvicos (60,0%) e nas fêmeas nos membros torácicos (57,1%). Nos membros torácicos, 17,1% (18/105) das alterações foram diagnosticadas proximais à articulação metacarpofalangeana, 14,3% (15/105) na articulação metacarpofalangeana e 68,6% (72/105) estavam localizadas distais à articulação metacarpofalangeana. Nos membros pélvicos, 78,8% (93/118) apresentaram a origem da dor na região do tarso, 17,8% (21/118) proximal ao tarso e 3,4% (4/ 118) distal ao tarso. As articulações interfalangeanas distais e intertarsiana distal/tarsometatarsiana são importantes fontes de dor e inflamação e estiveram mais frequentemente envolvidas em claudicações de membro torácico e pélvico, respectivamente. Os cavalos Crioulos apresentam problemas de claudicação semelhantes aos descritos em outras raças que participam de provas de rodeio. Palavras-chave: claudicação, cavalos crioulos, sistema locomotor. ABSTRACT This study aimed to identify the source of lameness in Criollo horses that are competing in the most important discipline for this breed in Southern Brazil and also in reining competitions. Clinical records of adult Criollo horses (n=201), 5.62.1 years of age, that underwent lameness examination due to history of poor performance or lameness between 2002/ 2009 were analyzed. Lameness was diagnosed in the front limbs in 47.1% (n=105) and in the hind limbs 52.9% of the cases (n=118). In males 60.0% of the lameness cases were located in the hind limbs. In females 57.1% of the lameness cases were located in the front limbs. In the front limbs (47.1%, n=105/201), problems proximal to the fetlock were diagnosed in 17.1% (18/105). Fetlock problems were diagnosed in 14.3% (15/105) and lesions located distal to the fetlock level were seen in 68.6% (72/105) of the lameness cases diagnosed in the front limbs. In the hind limbs, problems located proximal to the tarsus were seen in 17. 8% (21/118), 78.8% (93/118) in the tarsus and 3.4% (4/118) of the cases were seen distal to the tarsus. The distal interfalangeal joint in the front limbs and the distal tarsal joints in the hind limbs were the two most important sources of lameness. Criollo horses presented similar lameness problems as described in other show horses (reining, cutting and roping). This is the first lameness study done in Criollo horses and it will help to better understand the musculoskeletal diseases affecting this breed.

Research paper thumbnail of Serological status of mares in parturition and the levels of antibodies (IgG) against protozoan family Sarcocystidae from their pre colostral foals

Veterinary Parasitology, 2014

Protozoa from the family Sarcocystidae are agents of reproductive and neurological disorders in h... more Protozoa from the family Sarcocystidae are agents of reproductive and neurological disorders in horses. The transmission of these protozoa may occur via horizontal or vertical means, and the frequency and potential of the later is not fully elucidated in horses. Thus, the aim of study was to correlation levels of antibodies in mares with pre colostral foals seropositive and assess the level and distribuition of antibodies against Neospora spp., Sarcocystis neurona and Toxoplasma gondii, in mares and pre colostral foals at the parturition. The blood samples were collected from mares immediately after parturition and from newborns before the ingestion of colostrum, and sera were analyzed for the presence of IgG by ELISA. It was found that 21.5%, 33.7% and 27.6% of mares were seropositive for Neospora spp., S. neurona and T. gondii, respectively; foals had antibodies at a rate of 8.3%, 6.6% and 6.6% for Neospora spp., S. neurona and T. gondii, respectively. Additionally, paired samples from mares and pre-colostral foals revealed an overall negative correlation between the serum reactivity against these three parasites and suggested that seronegative mares, or those with low to intermediate antibody levels, have a higher risk of giving birth to seropositive foals.

Research paper thumbnail of Bioavailability and Tolerability of Topical and Oral Diclofenac Sodium Administration in Healthy Ponies

Journal of Equine Veterinary Science, 2013

Aiming to characterize the bioavailability and assess the safety of topical and oral treatment of... more Aiming to characterize the bioavailability and assess the safety of topical and oral treatment of diclofenac sodium in healthy ponies, 18 animals were divided in three groups: one treated with topical (group I, n ¼ 6), the second with oral diclofenac (group II, n ¼ 6) at 2.5 mg/kg for 3 days, and the third group received 2.2 mg/kg oral phenylbutazone (group III, n ¼ 6) also for 3 days to serve as positive control. To evaluate bioavailability, blood samples were collected before and at 0, 0.5, 1, 3, 36, 48, 60, 72, 96, 120, and 144 hours after starting treatment. To evaluate diclofenac sodium concentration in the synovial fluid, samples of six ponies (group I, n ¼ 3; group II, n ¼ 3) were collected at 6, 12, and 24 hours after starting the treatment.

Research paper thumbnail of The importance of vertical transmission of Neospora sp. in naturally infected horses

Veterinary Parasitology, 2012

Neospora spp. is a intracellular protozoan phylogenetically closely related to Toxoplasma gondii ... more Neospora spp. is a intracellular protozoan phylogenetically closely related to Toxoplasma gondii and Sarcocystis neurona, and it can infect horses leading to the development of reproductive or neurological diseases. We determined the presence of antibodies to Neospora sp. in mares at their parturition time and determine the frequency of vertical transmission in healthy foals to verify the importance of transplacental transmission. The samples were analyzed by indirect immunofluorescence antibody test, showing that seroprevalence in mares is higher than in foals and seropositive mares are likely to transmit the neosporosis to their offspring. This shows that endogenous challenge occurs in horses, and it suggests that this protozoan can be disseminated by means of transplacental transmission in horse species.

Research paper thumbnail of Anticorpos anti-Neospora spp. em amostras sorológicas de potros pré-colostrais pela técnica de imunofluorescência indireta

Ciência Rural, 2012

ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora s... more ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora spp. in samples of presuckle foal, as weel as determine the best dilution of serum to be used in indirect fluorescent antibody test. We analyzed serum samples from 203 presuckle foals, by indirect fluorescent antibody test in different titrations. The titrations of 16 and 50 showed 25.1% and 9.9% of presuckle foals positive, respectively. Thus, in serum samples from presuckle foals the titration 16 can be considered more appropriate to detect the occurrence of infection by the protozoan and therefore the transplacental transmission of Neospora spp. in horses.

Research paper thumbnail of Anticorpos anti-Neospora spp. em amostras sorológicas de potros pré-colostrais pela técnica de imunofluorescência indireta

Ciência Rural, 2012

ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora s... more ABSTRACT The objective of this study was to detect the frequency of antibodies against Neospora spp. in samples of presuckle foal, as weel as determine the best dilution of serum to be used in indirect fluorescent antibody test. We analyzed serum samples from 203 presuckle foals, by indirect fluorescent antibody test in different titrations. The titrations of 16 and 50 showed 25.1% and 9.9% of presuckle foals positive, respectively. Thus, in serum samples from presuckle foals the titration 16 can be considered more appropriate to detect the occurrence of infection by the protozoan and therefore the transplacental transmission of Neospora spp. in horses.

Research paper thumbnail of Claudicação em cavalos Crioulos atletas

Ciência Rural, 2011

O objetivo deste trabalho foi identificar as principais causas de claudicação em cavalos Crioulos... more O objetivo deste trabalho foi identificar as principais causas de claudicação em cavalos Crioulos em treinamento para a prova do Freio de Ouro e competições de rédeas. Foram avaliados os registros clínicos de 201 cavalos Crioulos, 5,62,1 anos de idade, submetidos a exame físico devido à presença de claudicação ou histórico de baixo rendimento atlético entre os anos de 2002 a 2009. A claudicação foi localizada nos membros torácicos e pélvicos em 47,1% (n=105) e 52,9% (n=118), respectivamente. Nos machos, houve uma predominância de claudicação nos membros pélvicos (60,0%) e nas fêmeas nos membros torácicos (57,1%). Nos membros torácicos, 17,1% (18/105) das alterações foram diagnosticadas proximais à articulação metacarpofalangeana, 14,3% (15/105) na articulação metacarpofalangeana e 68,6% (72/105) estavam localizadas distais à articulação metacarpofalangeana. Nos membros pélvicos, 78,8% (93/118) apresentaram a origem da dor na região do tarso, 17,8% (21/118) proximal ao tarso e 3,4% (4/ 118) distal ao tarso. As articulações interfalangeanas distais e intertarsiana distal/tarsometatarsiana são importantes fontes de dor e inflamação e estiveram mais frequentemente envolvidas em claudicações de membro torácico e pélvico, respectivamente. Os cavalos Crioulos apresentam problemas de claudicação semelhantes aos descritos em outras raças que participam de provas de rodeio. Palavras-chave: claudicação, cavalos crioulos, sistema locomotor. ABSTRACT This study aimed to identify the source of lameness in Criollo horses that are competing in the most important discipline for this breed in Southern Brazil and also in reining competitions. Clinical records of adult Criollo horses (n=201), 5.62.1 years of age, that underwent lameness examination due to history of poor performance or lameness between 2002/ 2009 were analyzed. Lameness was diagnosed in the front limbs in 47.1% (n=105) and in the hind limbs 52.9% of the cases (n=118). In males 60.0% of the lameness cases were located in the hind limbs. In females 57.1% of the lameness cases were located in the front limbs. In the front limbs (47.1%, n=105/201), problems proximal to the fetlock were diagnosed in 17.1% (18/105). Fetlock problems were diagnosed in 14.3% (15/105) and lesions located distal to the fetlock level were seen in 68.6% (72/105) of the lameness cases diagnosed in the front limbs. In the hind limbs, problems located proximal to the tarsus were seen in 17. 8% (21/118), 78.8% (93/118) in the tarsus and 3.4% (4/118) of the cases were seen distal to the tarsus. The distal interfalangeal joint in the front limbs and the distal tarsal joints in the hind limbs were the two most important sources of lameness. Criollo horses presented similar lameness problems as described in other show horses (reining, cutting and roping). This is the first lameness study done in Criollo horses and it will help to better understand the musculoskeletal diseases affecting this breed.

Research paper thumbnail of Serological status of mares in parturition and the levels of antibodies (IgG) against protozoan family Sarcocystidae from their pre colostral foals

Veterinary Parasitology, 2014

Protozoa from the family Sarcocystidae are agents of reproductive and neurological disorders in h... more Protozoa from the family Sarcocystidae are agents of reproductive and neurological disorders in horses. The transmission of these protozoa may occur via horizontal or vertical means, and the frequency and potential of the later is not fully elucidated in horses. Thus, the aim of study was to correlation levels of antibodies in mares with pre colostral foals seropositive and assess the level and distribuition of antibodies against Neospora spp., Sarcocystis neurona and Toxoplasma gondii, in mares and pre colostral foals at the parturition. The blood samples were collected from mares immediately after parturition and from newborns before the ingestion of colostrum, and sera were analyzed for the presence of IgG by ELISA. It was found that 21.5%, 33.7% and 27.6% of mares were seropositive for Neospora spp., S. neurona and T. gondii, respectively; foals had antibodies at a rate of 8.3%, 6.6% and 6.6% for Neospora spp., S. neurona and T. gondii, respectively. Additionally, paired samples from mares and pre-colostral foals revealed an overall negative correlation between the serum reactivity against these three parasites and suggested that seronegative mares, or those with low to intermediate antibody levels, have a higher risk of giving birth to seropositive foals.

Research paper thumbnail of Bioavailability and Tolerability of Topical and Oral Diclofenac Sodium Administration in Healthy Ponies

Journal of Equine Veterinary Science, 2013

Aiming to characterize the bioavailability and assess the safety of topical and oral treatment of... more Aiming to characterize the bioavailability and assess the safety of topical and oral treatment of diclofenac sodium in healthy ponies, 18 animals were divided in three groups: one treated with topical (group I, n ¼ 6), the second with oral diclofenac (group II, n ¼ 6) at 2.5 mg/kg for 3 days, and the third group received 2.2 mg/kg oral phenylbutazone (group III, n ¼ 6) also for 3 days to serve as positive control. To evaluate bioavailability, blood samples were collected before and at 0, 0.5, 1, 3, 36, 48, 60, 72, 96, 120, and 144 hours after starting treatment. To evaluate diclofenac sodium concentration in the synovial fluid, samples of six ponies (group I, n ¼ 3; group II, n ¼ 3) were collected at 6, 12, and 24 hours after starting the treatment.

Research paper thumbnail of The importance of vertical transmission of Neospora sp. in naturally infected horses

Veterinary Parasitology, 2012

Neospora spp. is a intracellular protozoan phylogenetically closely related to Toxoplasma gondii ... more Neospora spp. is a intracellular protozoan phylogenetically closely related to Toxoplasma gondii and Sarcocystis neurona, and it can infect horses leading to the development of reproductive or neurological diseases. We determined the presence of antibodies to Neospora sp. in mares at their parturition time and determine the frequency of vertical transmission in healthy foals to verify the importance of transplacental transmission. The samples were analyzed by indirect immunofluorescence antibody test, showing that seroprevalence in mares is higher than in foals and seropositive mares are likely to transmit the neosporosis to their offspring. This shows that endogenous challenge occurs in horses, and it suggests that this protozoan can be disseminated by means of transplacental transmission in horse species.