Ercument Olmez - Academia.edu (original) (raw)

Papers by Ercument Olmez

Turkiye Klinikleri Journal of Medical Sciences, 2010

Melatonin, a hormone produced by the pineal gland, has been suggested to protect against developm... more Melatonin, a hormone produced by the pineal gland, has been suggested to protect against development of hypertension and atherosclerosis. In this study, the effects of longterm melatonin deficiency for twelve months after pinealectomy on the α-adrenergic-contractions induced by phenylephrine, endothelium-dependent relaxation responses to acetylcholine and the morphological changes in the rat thoracic aorta were studied. M Ma at te er ri ia al l a an nd d M Me et to od ds s: : Rats were pinealectomized twelve months before the beginning of the vasomotor studies. Rings of arteries were mounted in isolated tissue baths for the measurements of isometric contractile force. The contractile responses to phenylephrine and endothelium-dependent relaxation responses to acetylcholine in the vessels were evaluated. Endothelial function was evaluated by vascular relaxation to acetylcholine. Histological examinations demonstrated the alterations of tunica media in the vessels of pinealectomized rats. R Re es su ul lt ts s: : Thick and thin areas were observed in the transverse sections of vessels and the ratio of the widest media thickness to the narrowest was found significantly increased in pinealectomized group (2.85 ± 0.56) when compared to the control group (1.65 ± 0.10). In addition, α-smooth muscle actin and elastic lamellae staining of the media were attenuated in pinealectomized rats. Although contractile responses of vessels to phenylephrine in pinealectomized rats were lower than control group, significant difference was found for only one concentration (3x 10-8 mol l-1) of phenylephrine. There was no difference between the relaxation responses to acetylcholine in pinealectomized and control groups. C Co on nc cl lu us si io on n: : These results show that long-term melatonin deficiency may cause some morphological changes in the tunica media of vessels. However, the function of endothelium and vascular responsiveness to ∝-adrenergic stimulus seem to be mostly protected. K Ke ey y W Wo or rd ds s: : Melatonin; aorta, thoracic; rats Ö ÖZ ZE ET T A Am ma aç ç: : Pineal bezden üretilen bir hormone olan melatoninin hipertansiyon ve ateroskleroza karşı koruyucu olduğu ileri sürülmüştür. Bu çalışmada sıçan torasik aortalarında, pinealektomi sonrası on iki ay süreyle uzun süreli melatonin eksikliğinin, fenilefrinle oluşturulan α-adrenerjik kasılmalar, asetilkolinle oluşan endotelyuma bağlı gevşemeler ve morfolojik değişiklikler üzerine etkileri çalışıldı. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Sıçanların pineal bezleri vazomotor çalışmaların başlamasından on iki ay önce çıkarıldı. Arter halkaları izometrik kasılma gücün ölçümü için izole doku banyosuna asıldı. Damarlarda fenilefrinle oluşturulan kasılma cevapları ve asetilkolinle oluşan endotelyuma bağlı gevşeme cevapları değerlendirildi. Pinealektomize sıçanların damarlarındaki tunika media tabakasının değişiklikleri histolojik olarak muayene edildi. B Bu ul lg gu ul la ar r: : Kontrol grubu (1.65 ± 0.10) ile karşılaştırıldığında damarların transvers kesitlerinden elde edilen kalın ve ince alanlar ve media tabakasının en geniş kalınlığının en dara oranı pinealektomize grupta (2.85 ± 0.56) anlamlı olarak artmış bulundu. Ayrıca, medianın α-düz kas aktin ve elastik lamel boyaması pinealektomize sıçanlarda azaldı. Her ne kadar pinealektomize sıçanlardaki arterlerin fenilefrine kasılma cevapları kontrol grubundan daha düşük olsa da fenilefrinin yanlızca bir konsantrasyonunda (3x 10-8 mol l-1) anlamlı farklılık bulundu. Pinealektomize ve kontrol grupların asetilkoline gevşeme cevapları arasında anlamlı farklılık yoktu. S So on nu uç ç: : Bu sonuçlar uzun süreli melatonin eksikliğinin damarların tunika mediasında bazı morfolojik değişikliklere neden olabileceğini göstermektedir. Bununla birlikte endotelyumun fonksiyonu ve ∝-adrenerjik uyarılara damarsal cevap verirlilik çoğunlukla korunmuş gibi görünmektedir.

Medicine Science | International Medical Journal, 2019

Breast cancer today is the most frequent cancer among women, and the second most common cause of ... more Breast cancer today is the most frequent cancer among women, and the second most common cause of cancer deaths among women. The aim of this study was to synthesize a new benzoxazole derivative, scan it for anti-cancer potential by MTT test using different breast cancer cell lines, and examine its effects on NF-κB and apopitosis-related proteins (APAF-1, cytochrome C, caspase-3, bcl-2) by the western blot method. newly-synthesized benzoxazole compound was applied to breast cancer cell lines (MDA-MB, MCF-7) and its cytotoxicity was measured quantitatively by MTT test. Later, the level of its effects on NF-κB and apopitosis-related proteins (APAF-1, cytochrome C, caspase-3, bcl-2) were examined by the western blot method. In our study, the structure of the synthesized new 5-[4-chlorobutanamido]-2-(p-methylphenyl)benzoxazole was proved by elemental analysis, 1H NMR and mass spectroscopy analysis methods. When the toxic effects of the application of the compound on the cell lines was examined by MTT, it had a greater toxic effect on MCF-7 when compared with MDA-MB, and IC50 levels were lower. When the protein was examined in immunohistochemistry with regard to VEGF, eNOS and TUNEL, it was observed that it caused a reduction in VEGF and an increase in eNOS and TUNEL. In the assay of the proteins by western blot, when benzoxazole compound was added to the MDA and MCF-7 cell line, there was no difference from the control group in Apaf-1 and BCL-2 levels, but a reduction was observed in caspase and Nfkβ levels compared with the control group. When the compound was added to the MDA-MB cell line, an increase was shown in the Cytochrome C level compared to the control group, but no difference was seen in the MCF-7 cell line. It is felt that this synthesized new benzoxazole compound increases apopitosis by reducing the activation of Nfkβ, and in this way has shown an effect of inhibiting tumor growth in cancer treatment. In addition, it is felt that this can provide hope in cancer treatment by the improved phase studies.

This review is about clinical pharmacology of histamine H2-receptor antagonists and their prophyl... more This review is about clinical pharmacology of histamine H2-receptor antagonists and their prophylactic and therapeutic effects in peptic ulser disease, Zollinger-Ellison Syndrome, gastroesophageal reflux and acute stress ulcers and erosions which are known asid-peptic disorders. H2-receptor blocker drugs (Cimetidine, Ranitidine, Famotidine, Nizatidine) using clinically shows similar effects when they are equipotent doses. Cimetidine and in a lesser extent ranitidine could interact many drugs by inhibiting metabolism of these drugs. However, famotidine and nizatidine interact with other drugs lesser than former drugs. If a therapy is planned with H2-blocker drugs, interactions with other drugs, safety and price of these drugs should be considered. [Journal of Turgut Ozal Medical Center 1(1):53-64,1994]. Key words: Histamine, H2-receptor blockers, clinical pharmacology

JPMA. The Journal of the Pakistan Medical Association, 2021

OBJECTIVE To assess the effects of antidepressant use on pregnancy outcomes. METHODS The cross-se... more OBJECTIVE To assess the effects of antidepressant use on pregnancy outcomes. METHODS The cross-sectional study was conducted at the Department of Pharmacology, Manisa Celal Bayar University, Manisa, Turkey, and comprised pregnant women who were admitted to the Department of Gynaecology between 2008 and 2017 who had been prescribed antidepressant drugs before pregnancy and continued to use them during any week of their respective pregnancies. The women were contacted by telephone after delivery to obtain information about the pregnancy outcomes. Data was analysed using SPSS 23. RESULTS There were 183 women with a mean age of 31.3 ± 5.3 years (range: 18-44 years). There were congenital defects in the newborn in 11(7.65%) cases. The most commonly used antidepressant group was selective serotonin reuptake inhibitor 138(75.4%), and escitalopram was the most frequently used drug 46(25.1%). Spontaneous abortion rate was higher with escitalopram than the other antidepressants (p=0.062). Ind...

Aim: We aimed to investigate the myocardial effects of the addition of captopril, a sulphydryl -c... more Aim: We aimed to investigate the myocardial effects of the addition of captopril, a sulphydryl -containing angiotensin converting enzyme (ACE) inhibitor and lisinopril, a non -sulphydryl-containing ACE inhibitor to the prime solution and cardioplegia. Material and Methods: Isolated rat heart model was used for the study. Rats were allocated into three groups: a control group, lisinopril group and captopril group. For all groups, cardioplegic arrest was achieved following 20 minutes of perfusion and 30 minutes of ischemia with con tinuous cardioplegic infusion and 30 min of reperfusion following ischemia were performed. According to study group, lisinopril or captopril were added to the prime solution and cardioplegic solution. Coronary blood plow, cardiac oxygen consumption, CPK, L DH, AST secretions were measured in perfusion and reperfusion periods. Results: Both drugs, compared to the plasebo group, caused statistically significant increase in coronary blood flow during perfusion...

$Research paper thumbnail of Mesenchymal Stem Cells: a Potential Treatment Approach for Refractory Chronic Spontaneous Urticaria$

Stem Cell Reviews and Reports, 2020

The etiopathogenesis of chronic spontaneous urticaria (CSU) is not fully elucidated, and almost 3... more The etiopathogenesis of chronic spontaneous urticaria (CSU) is not fully elucidated, and almost 30-40% of patients are resistant to treatments; therefore, there is still a need for the development of new and effective treatments. This study aimed to develop experimental cellular therapy for CSU patients resistant to current treatment options. Autologous adipose tissue mesenchymal stem cells (MSC) were administered to 10 refractory CSU patients who were then followed up for six months. The efficacy of treatment was evaluated according to the weekly urticaria activity scores (UAS7) and drug use scores (DUS7). To observe the effect of treatment on immune cells, CD4 + T cell subsets were analyzed by flow cytometry, and the serum IFN-γ, TNF-α, IL2,

Journal of Health Services and Education, 2020

Gastric cancer has a high incidence and is one of the leading causes of mortality both in males a... more Gastric cancer has a high incidence and is one of the leading causes of mortality both in males and females. Angiogenesis is the formation of new capillaries from existing blood vessels through which oxygen and nutrients are transported to the tissues to promote growth, and it plays a pivotal role in the invasion and metastasis of cancer caused by oncogenes. Even though this process is useful in tissue repair and normal growth, it also facilitates the growth of cancer tissues and their metastasis via the hematogenous route, and in this way, it speeds up metastasis and invasion. In the present study, we aimed to investigate the relationship between angiogenesis and markers such as VEGF, MMP, TSP-1 and Endostatin in a CAPE-administered gastric cancer cell in culture. Following CAPE administration, cytotoxicity was measured by MTT test. Angiogenic behavior of cells was examined immunohistochemically by VEGF, MMP and Endostatin, and TSP-1 level was measured by RT-PCR test. MTT test showed that CAPE administered at a concentration of 0.5 µg/ mL exerted a cytotoxic effect on a gastric cancer cell line. Gene expression depicted by RT-PCR showed that VEGF, MMP and TSP levels were decreased , while the endostatin level was increased. Changes in MMP, VEGF, TSP and endostatin suggest that CAPE has a significant therapeutic effect on gastric cancer. In the light of these findings, CAPE, which is a natural and inexpensive substance, could be effective in the treatment of cancer patients and advanced studies need to be carried out.

Cancer Research, 2016

Introduction: Benzoxazole have received considerable attention during last few decades as they ar... more Introduction: Benzoxazole have received considerable attention during last few decades as they are endowed with variety of biological activities and have wide range of therapeutic properties. The present study aimed to evaluate cytotoxic and antiapoptotic activities of new synthesize benzoxazole derivative in breast cancer cell lines. Material and Method: In this study, the breast cancer cell lines MDA-MB and MCF-7 were used. Cytotoxic activities of novel benzoxazole-derived compound were analyzed with MTT assay. Additionally, the level of its effects on NF-κB and apoptosis-related proteins were examined by the western blot. Immunohistochemical stainings were done for VEGF, eNOS proteins and Tunnel assay was performed to show DNA damage. Results: The structure of the compound synthesized in our study 5-amino-2-(p-bromophenyl)-benzoxazole was proved by elemental analysis, IR, 1H NMR and mass spectroscopy analysis methods. Novel benzoxazole analogue detected cytotoxic on breast cancer...

KLINIK PSIKOFARMAKOLOJI BULTENI-BULLETIN OF CLINICAL PSYCHOPHARMACOLOGY, 2016

Safety of psychotropic medications in pregnancy: an observational cohort study Objective: The que... more Safety of psychotropic medications in pregnancy: an observational cohort study Objective: The question of harmfulness of the psychiatric drugs creates a major dilemma for pregnant women. The risks associated with prenatal psychotropic drug exposure are often overestimated. It is unclear that psychotropic medication or disorders themselves increase the risk of adverse pregnancy outcomes. The purpose of this study is to generate data about the safety of psychotropic drugs in pregnancy and maternal characteristics of the pregnant women exposed to these drugs. Method: An observational cohort study was performed. Pregnancy outcomes of 135 pregnancies after psychotropic drug exposure are compared to a control group of 275 pregnancies. Results: There were no statistically significant differences in rates of major malformations, miscarriages, and preterm deliveries between the two groups. However, the rate of elective abortions was higher in the exposed group compared to the control group (11.1% vs. 5.1%, respectively; RR 2.18; 95% CI: 1.09-4.39), and most of them were nulliparous (45.2%). The majority of the pregnant women did not smoke cigarettes and no alcohol consumption was reported in both groups. Conclusion: Our study showed that there was a tendency to terminate pregnancy among women exposed to psychotropic drugs. An accurate risk assessment about drug safety and informing pregnant women would help to prevent unnecessary terminations of pregnancies.

Reproductive toxicology (Elmsford, N.Y.), Apr 11, 2016

Thiocolchicoside is a commonly used muscle relaxant in orthopedic, rheumatologic or musculoskelet... more Thiocolchicoside is a commonly used muscle relaxant in orthopedic, rheumatologic or musculoskeletal disorders to treat painful muscle spasms. It is contraindicated in pregnancy and lactation. There is no previously published experience with thiocolchicoside exposure during pregnancy. In this observational study, we collected and evaluated 18 pregnancy outcomes of the women referred to our prenatal consultation service for thiocolchicoside exposure between 2007-2012, and offspring were followed up until 2 years of age. There were 16 live births, 1 spontaneous abortion and 1 elective termination of pregnancy. No major birth defect was observed. The mothers and their babies were free of perinatal complications. No growth or developmental abnormalities were found during follow-up period. Our findings add information on inadvertent use of thiocolchicoside in pregnancy. Further large prospective cohort studies are required to investigate this issue.

Turkiye Klinikleri Journal of Internal Medical Sciences, 2006

Reproductive Toxicology, 2014

Pharmacological Research, 2000

The effects of chronic oral administration of ethanol 7.2% daily during 24 weeks on the Ž. contra... more The effects of chronic oral administration of ethanol 7.2% daily during 24 weeks on the Ž. contractions induced by phenylephrine Phe and the endothelium-dependent relaxation Ž. responses to acetylcholine ACh were studied in rat thoracic aorta. Ethanol pretreatment significantly attenuated the contractile responses to Phe, resulting in parallel shift of the concentration᎐response curve to the right. EC values of Phe were 64.6" 11.2 and 50 95.5" 8.5 nmol l y1 in control and ethanol-fed rats, respectively. On the other hand, either calcium-induced contractions or relaxation responses to ACh and sodium nitroprusside were similar in the vessels of the control and ethanol-treated rats. These results suggest that chronic ethanol ingestion significantly attenuates the ␣-adrenergic-induced contrac-1 tions but does not affect the relaxation responses mediated by nitric oxide in rat aortic rings.

Pharmacological Research, 1995

Pharmacological Research, 2004

Pericardial fluid reflect the composition of cardiac interstitium in myocardial ischemia. This st... more Pericardial fluid reflect the composition of cardiac interstitium in myocardial ischemia. This study investigated the value of the pericardial and serum myoglobin (MG) measurements for the diagnosis of perioperative myocardial infarction (MI) after coronary artery bypass grafting (CABG). Postoperative arterial and pericardial blood samples were taken in 64 subjects undergoing elective CABG allocated to two groups according to the 12-lead electrocardiogram (ECG) abnormalities observed during the first postoperative 24 h. Group 1=normal and nonspesific ECG abnormalities, and Group 2=perioperative Q-wave MI. The occurrence of perioperative MI was associated with a dramatic increase in both serum and pericardial cardiac troponin I (CTnI) and MG concentrations. Pericardial concentrations were higher than serum concentrations during the first postoperative 24 h in all subject. However, pericardial/serum CTnI ratio in subjects in Group 2 was not statistically different from Group 1 at the time of admission to the intensive care unit (ICU) and did not significantly change at time intervals. On the other hand, more than twofold increase in the pericardial/serum MG ratio was determined for all patients who experienced perioperative Q-wave MI with the lowest value as 2.75, whereas only 1 of 59 patients in group 1 had the ratio higher than 2 with the highest value as 2.15 at the time of admission to the ICU. In conclusion, determination of pericardial/serum MG ratio may be a useful tool for the early diagnosis of the perioperative MI after CABG.

Pharmacological Research, 1997

Attenuation of ischaemia-reperfusion induced arrhythmias by several angiotensin converting enzyme... more Attenuation of ischaemia-reperfusion induced arrhythmias by several angiotensin converting enzyme (ACE) inhibitors, such as captopril, has been demonstrated. The role of prostaglandin synthesis stimulation in this protective effect of ACE inhibition was evaluated in an in vivo rat model. To produce arrhythmia, the left main coronary artery was occluded for 7 min, followed by 7 min of reperfusion. Captopril (3 mg kg-1) and a prostaglandin synthesis inhibitor, indomethacin (2 mg kg-1) alone or together were administered by intravenous (i.v.) injection 10 min before occlusion. Captopril reduced the incidence of ventricular tachycardia (VT) and the number of ventricular ectopic beats (VEB) on ischaemia and reperfusion as well as the incidence of reversible ventricular fibrillation (VF) on reperfusion. These protective effects of captopril against ischaemia-reperfusion-induced arrhythmias were prevented by indomethacin. Captopril also caused a sustained decrease of preocclusion values in the arterial blood pressure (BP) and heart rate (HR), whereas in the presence of indomethacin, captopril had no significant effect on either HR or arterial BP values except the heart rate value just before occlusion. Indomethacin alone did not affect either the severity of arrhythmias or the haemodynamic parameters. These results suggest that, in this experimental model, the protective effects of ACE inhibitors on the arrhythmias following ischaemia-reperfusion are mediated by the stimulation of prostaglandin synthesis and the haemodynamic effects of these drugs may have a contributory role in their protective effect.

Journal of Pineal Research, 2002

Journal of Pharmacological Sciences, 2006

In this study, the effects of reduced melatonin concentrations in the long-term period of pineale... more In this study, the effects of reduced melatonin concentrations in the long-term period of pinealectomy on mean arterial blood pressure (BP) and vascular responses in isolated rat thoracic aorta were investigated. Rats were pinealectomized (Px) two months before the beginning of the studies. Rings of endothelium-intact and-denuded rat arteries were mounted in isolated tissue baths for the measurements of isometric contractile force. No significant difference was determined between the arterial BP of Px (88.1 ± 1.9 mmHg) and control (83.8 ± 1.2 mmHg) rats. All arteries isolated from control and Px rats showed essentially identical contractions in response to phenylephrine, serotonin, calcium, clonidine, vasopressin, and angiotensin-II. Only endothelin-1 (ET-1)-induced contractions in the endothelium-denuded vessels isolated from Px rats were found to be increased to some extent. Pinealectomy did not affect acetylcholine or sodium nitroprusside-induced relaxation in the rat aorta either. These data suggest that reduced melatonin levels two months after pinealectomy did not modify either the vascular reactivity to various vasoconstrictor agents except the partially increased contractile responses to ET-1 in the endothelium-denuded thoracic aortas of Px rats or the endotheliumdependent and-independent relaxations in rat thoracic aorta. Restoration of the increased vascular responses to some vasoconstrictor agents, which were reported previously, may be the reason of why the hypertension is temporary following pinealectomy.

Indian Journal of Urology, 2010

Aim: Aim: This study was designed to test the hypothesis that propofol, ketamine, and midazolam c... more Aim: Aim: This study was designed to test the hypothesis that propofol, ketamine, and midazolam could alter the contractile activity of detrusor smooth muscle. Materials and Methods: Materials and Methods: Four detrusor muscle strips isolated from each rat bladder (n = 12) were placed in 4 tissue baths containing Krebs-Henseleit solution. The carbachol (10-8 to 10-4 mol/L)-induced contractile responses as well as 5, 10, 20, 30, 40, 50 Hz electrical fi eld stimulation (EFS)-evoked contractile responses of the detrusor muscles were recorded using isometric contraction measurements. After obtaining basal responses, the in vitro effects of propofol, ketamine, midazolam (10 −5 to 10 −3 mol/L), and saline on the contractile responses of the detrusor muscle strips were recorded and evaluated. Results: Results: All the 3 drugs reduced the carbachol-induced and/or EFS-evoked contractile responses of rat detrusor smooth muscles in different degrees. Midazolam (10 −4 to 10 −3 mol/L) caused a signifi cant decrease in the contractile responses elicited by either EFS or carbachol (P=0.000-0.013). Propofol (10 −3 mol/L) caused a decrease only in EFS-evoked contractile responses (P=0.001-0.004) and ketamine (10 −3 mol/L) caused a decrease only in carbachol-induced contractile responses (P=0.001-0.034). Conclusion: Conclusion: We evaluated the effects of the 3 different intravenous anesthetics on detrusor contractile responses in vitro and found that there are possible interactions between anesthetic agents and detrusor contractile activity. The depressant effects of midazolam on the contractile activity were found to be more signifi cant than ketamine and propofol. Despite the necessity of further studies, it could be a piece of wise advice to clinicians to keep the probable alterations due to intravenous anesthetics in mind, while evaluating the results of urodynamic studies in children under sedation.