Erik Nedergaard Hansen - Academia.edu (original) (raw)

Papers by Erik Nedergaard Hansen

Clinical Orthopaedics and Related Research, Feb 1, 2011

Background Evidence suggests a growing incidence of revision total hip arthroplasty (THA) includi... more Background Evidence suggests a growing incidence of revision total hip arthroplasty (THA) including a subset with large acetabular defects. Revision THA for severe acetabular bone loss is associated with a relatively high rate of mechanical failure. Questions/purposes We questioned whether cementing a cage to the reconstructed acetabular defect and pelvis would improve the rate of mechanical failure for patients with Type 3 defects (Paprosky et al.) with and without pelvic discontinuity in comparison to historical controls. Methods We retrospectively collected data on 33 patients who underwent 35 revision THAs using an acetabular reconstruction cage cemented to morselized allograft and either structural allograft or trabecular metal augmentation for Type 3 defects in the presence (n = 13) and absence (n = 22) of pelvic discontinuity at a mean followup of 59 months (range, 24-92 months). The primary outcome was mechanical failure, defined as revision of the acetabular reconstruction for aseptic loosening. Results Revision surgery for mechanical failure occurred in four of the 13 patients with pelvic discontinuity and two of the 22 patients without discontinuity. Radiographic loosening occurred in one patient with and one patient without pelvic discontinuity. Seven of the 35 revisions were subsequently revised for deep infection all in patients who were immunocompromised. Conclusions Cementing the cage to the pelvis can offer an advantage for treating severe acetabular defects. Trabecular metal augmentation appears to provide better initial mechanical stability than a structural allograft, but successful allograft reconstruction may restore bone stock. Level of Evidence Level IV, therapeutic study. See Guidelines for Authors for a complete description of levels of evidence. One or more of the authors (MDR) is a consultant for Smith & Nephew. Each author certifies that his institution either has approved or waived approval for the human protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.

European Journal of Pharmacology, Nov 21, 1989

Abstract he release of vasopressin from the isolated superfused rat neurohypophysis was measured.... more Abstract he release of vasopressin from the isolated superfused rat neurohypophysis was measured. The electrically eveoked release of vasopressin after phasic submaximal stimulation was increased on exposure to the cytokine, interleukin-1β (44 pM). The release returned to its control level when the peptide was withdrawn. The results indicates a permissive role of interleukin-1β in the release of vasopressin.

Pharmacology Amp Toxicology, Jun 1, 1992

Scandinavian Journal of Gastroenterology, Mar 1, 1999

Endoscopic ultrasound (EUS) is a new technique that makes it possible to measure portal venous fl... more Endoscopic ultrasound (EUS) is a new technique that makes it possible to measure portal venous flow when transabdominal ultrasound fails. As the technique has not been evaluated previously, we compared simultaneous measurements of portal venous flow using EUS Doppler with that of transit time ultrasound (TTU) in healthy pigs. The ability of EUS to detect changes in the portal venous flow after pharmacologic intervention was also investigated. Six anaesthetized pigs were studied. Portal venous flow was measured simultaneously by EUS duplex scanning, using a Pentax FG-32UA echoendoscope connected to a Hitachi EUB 515-A ultrasound scanner, and by TTU with a Cardiomed CM 4000 flowmeter probe placed on the portal vein. Terlipressin, 1 mg, and placebo were administered in a blind, randomized, crossover design. Measurements were taken at base line and 30 min after each drug administration. Portal venous flow measured by EUS flowmetry and TTU flowmetry correlated significantly (R = 0.92, P < 0.001, n = 18). The limits of agreement in a Bland-Altman plot were from -0.262 l/min to +0.164 l/min. The change in portal venous flow after terlipressin measured by EUS and TTU correlated significantly (R = 0.88, P < 0.05, n = 6). All values used for analyses were the means of five repeated measurements. EUS measures portal venous flow with an acceptable correlation and agreement with TTU in anaesthetized pigs. The method is capable of monitoring the effects of pharmacologic intervention on the portal system.

Clinical Orthopaedics & Related Research, 2016

Background Complex revision total knee arthroplasty (TKA) often calls for endoprosthetic reconstr... more Background Complex revision total knee arthroplasty (TKA) often calls for endoprosthetic reconstruction to address bone loss, poor bone quality, and soft tissue insufficiency. Larger amounts of segmental bone loss in the setting of joint replacement may be associated with greater areas of devascularized tissue, which could increase the risk of complications and worsen functional results. Questions/purposes Are longer endoprosthetic reconstructions associated with (1) higher risk of deep infection; (2) increased risk of reoperation and decreased implant survivorship; or (3) poorer ambulatory status? Methods This is a single-institution retrospective case series of nononcologic femoral endoprosthetic reconstructions for revision TKA from 1995 to 2013 (n = 32). Cases were categorized as distal (n = 17) or diaphyseal (n = 15) femoral reconstructions based on extension to or above the supracondylar metaphyseal-diaphyseal junction, respectively. Five patients from each group were lost to followup One of the authors (MR), or a member of his or her immediate family, has or may receive payments or benefits, during the study period, an amount of more than USD 1,000,001, from Smith & Nephew, Inc (Memphis, TN, USA). All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research1 editors and board members are on file with the publication and can be viewed on request. Each author certifies that his or her institution approved the human protocol for this investigation, that all investigations were conducted in conformity with ethical principles of research, and that informed consent for participation in the study was obtained.

Journal of Clinical Pharmacy and Therapeutics, Aug 1, 2000

Pharmaceutical products containing proteins cause problems in testing for endotoxin and pyrogens.... more Pharmaceutical products containing proteins cause problems in testing for endotoxin and pyrogens. Many proteins interfere with the LAL test and the proteins are immunogenic in rabbits. The monocytic cell line Mono Mac 6 is an alternative assay for detection of endotoxin and other pyrogens. To evaluate the use of the Mono Mac 6 assay for quantitative detection of endotoxin in proteins. The quantitative detection of endotoxin in the three pharmaceutical products human albumin, gamma-globulin and somatropin was evaluated. For the three proteins the detection limit of the Mono Mac 6 assay was far below the threshold endotoxin limit described by the European Pharmacopoeia. Interference of two of the proteins with the Mono Mac 6 assay was observed, but the problems could be overcome either by dilution of the product or by comparison of the test with an endotoxin standard curve prepared in a solution of the respective pyrogen-free protein. The Mono Mac 6 assay is a reliable method for quantitative detection of endotoxin in proteins.

The Annals of occupational hygiene, 2014

The bacterium Bacillus thuringiensis (Bt) is the active organism in a variety of commercially ava... more The bacterium Bacillus thuringiensis (Bt) is the active organism in a variety of commercially available products used worldwide as biopesticides. Bt products are applied in large outdoor areas as well as in indoor environments. Even though it has been sold for decades, not much is known about the occupational exposure to Bt. The aim of this study was to obtain knowledge about the exposure to Bt subspecies israelensis (Bti) in a propagation section in a greenhouse, where Bti is applied hourly by a spray boom, and to test a preventive measure to reduce the exposure to airborne Bti. Furthermore, we wanted to study the exposure during work with potted plants treated earlier with Bti. Exposure to aerosols with Bti was measured repeatedly by personal and stationary samplers before and after the intervention. Bti was identified by polymerase chain reaction in air and soil samples. Personal exposure to inhalable Bti in the propagation section was 3×10(5) cfu m(-3) (median level, n = 22); th...

Scour and scour protection is a major issue for the construction of offshore wind farms. The engi... more Scour and scour protection is a major issue for the construction of offshore wind farms. The engineer can either include the scour in his design or he can place a scour protection on the seabed. The optimal solution is highly dependent on the maximal scour depth an unprotected foundation will experience during its lifetime. Today's design practice has not yet been defined for foundations placed in areas with breaking waves. Some engineers include possible wave breaking in the design by increasing scour depth for current alone. So that the design scour depth is considered to be more than 1.5 times the pile diameter. The present study includes some study of scour processes in physical models as well as in a numerical model, under the influence of breaking and broken waves. Only minor influence on the scour depths are observed for the breaking waves.

Pharmaceutisch Weekblad, 1989

Supplement to Pharmaceutisch Weekblad Scientific edition, volume 11, number 5, 20 October 1989 ..... more Supplement to Pharmaceutisch Weekblad Scientific edition, volume 11, number 5, 20 October 1989 ... Organized by the American College of Clinical Pharmacy, the Dutch Society of Clinical Pharmacology and Biopharmacy and the WHO Collaborating Centre for Quality Control in ...

British Journal of Nutrition, 2008

Dietary long-chain n-3 PUFA (n-3 LCPUFA) are thought to have immune-modulating effects, but the s... more Dietary long-chain n-3 PUFA (n-3 LCPUFA) are thought to have immune-modulating effects, but the specific effects and mechanisms are not fully elucidated. The aim of this study was to determine whether dietary n-3 LCPUFA could affect ex vivo oxidative burst in healthy young men. The study had a randomised 2 × 2-factorial design in which subjects were randomly assigned to 8-week supplementation with capsules containing fish oil (about 2·9 g n-3 LCPUFA/d) or olive oil (control). Subjects were also randomly assigned to household use of oils and fat spreads with a high or a low 18 : 2n-6 content. At baseline and at the end of the intervention, the fatty acid composition of peripheral blood mononuclear cells (PBMC) was analysed by GLC and oxidative burst was studied in whole blood stimulated with zymosan using luminol-enhanced chemiluminescence. The PBMC content of n-3 LCPUFA was markedly increased by the fish oil-supplementation (P < 0·001, compared to the olive oil groups). No effect...

Soil Use and Management, 2014

Crop yields and yield potentials on Danish coarse sandy soils are strongly limited due to restric... more Crop yields and yield potentials on Danish coarse sandy soils are strongly limited due to restricted root growth and poor water and nutrient retention. We investigated if biochar amendment to subsoil can improve root development in barley and significantly increase soil water retention. Spring barley (Hordeum vulgare cv. Anakin) was grown in soil columns (diameter: 30 cm) prepared with 25 cm topsoil, 75 cm biochar-amended subsoil, and 30 cm un-amended subsoil lowermost placed on an impervious surface. Low-temperature gasification straw-biochar (at 0, 0.50, 1.0, 2.0, and 4.0 wt%) and slow pyrolysis hardwood-biochar (at 2 wt%) were investigated. One wt% can be scaled up to 10 2 Mg/ha of char. After full irrigation and drainage, the in-situ moisture content at 30-80 cm depth increased linearly (R 2 = 0.99) with straw-biochar content at a rate corresponding to 0.029 m 3 /m 3 /%. The lab determined wilting point also increased linearly with char content (R 2 = 0.99) but at a much lower rate (0.003 m 3 /m 3 /%). Biochar at concentrations up to 2% significantly increased the density of roots in the 40-80 cm depth interval. Addition of 1% straw-biochar had the most positive effect on root penetration resulting in the highest average root density (54% coverage compared to 33% without biochar). This treatment also resulted in the greatest spring barley grain yield increase (22%). Improving the quality of sandy subsoils has global potentials, and incorporation of the right amount of correctly treated residues from bioenergy technologies such as straw-biochar is a promising option.

Scandinavian Journal of Gastroenterology, 2012

Carvedilol is a non-selective β-blocker with intrinsic anti-α(1)-adrenergic activity, potentially... more Carvedilol is a non-selective β-blocker with intrinsic anti-α(1)-adrenergic activity, potentially more effective than propranolol in reducing hepatic venous pressure gradient (HVPG). We compared the long-term effect of carvedilol and propranolol on HVPG and assessed whether the acute response to oral propranolol predicted the long-term HVPG response on either drug. HVPG was measured in 38 patients with cirrhosis and HVPG ≥ 12 mm Hg at baseline and then again 90 min after an oral dose of 80 mg propranolol. Patients were double-blinded randomized to either carvedilol (21 patients) or propranolol (17 patients) and after 90 days of treatment HVPG measurements were repeated. HVPG decreased by 19.3 ± 16.1% (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01) and by 12.5 ± 16.7% (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01) in the carvedilol and propranolol groups, respectively, with no significant difference between treatment regimens (p = 0.21). Although insignificant, an acute decrease in HVPG of ≥12% was the best cut-off value to predict long-term HVPG response to propranolol when using ROC curve analysis. This randomized study showed that carvedilol is at least as effective as propranolol on HVPG after long-term administration. Furthermore, a predictive value of an acute propranolol test on HVPG could not be confirmed.

Phytotherapy Research, 2010

Hyben Vital International ApS is thanked for financial support.

The major challenge of ensuring the stability of the seabed around offshore wind turbine foundati... more The major challenge of ensuring the stability of the seabed around offshore wind turbine foundations is addressed in the present paper. Full-scale, high-resolution measurements of the seabed and the scour protection around the monopile foundations in Horns Rev offshore wind farm are analysed and presented. The measured developments in the scour protection are related to wave and current conditions. The wind turbines at Horns Rev wind farm are each placed on circular monopiles at water depths ranging from 6-13 m at MSL. The seabed consists of fine to coarse sand (D50 = 0.15-1 mm). The wave conditions are severe - the largest waves are in the order of 8 m high. The max current in the wind farm area is approximately 0.88 m/s (50 year return period). The stability of the seabed around the wind turbine foundations is one of the major challenges in offshore wind farm design. Each foundation at Horns Rev is protected by a 50 cm thick filter layer (D50 = 10 cm) and a 1 m thick armour layer ...

Journal of Waterway, Port, Coastal, and Ocean Engineering, 1995

ABSTRACT

Journal of Medicinal Chemistry, 2009

We describe a novel fragment library termed fragments of life (FOL) for structure-based drug disc... more We describe a novel fragment library termed fragments of life (FOL) for structure-based drug discovery. The FOL library includes natural small molecules of life, derivatives thereof, and biaryl protein architecture mimetics. The choice of fragments facilitates the interrogation of protein active sites, allosteric binding sites, and protein-protein interaction surfaces for fragment binding. We screened the FOL library against leukotriene A4 hydrolase (LTA4H) by X-ray crystallography. A diverse set of fragments including derivatives of resveratrol, nicotinamide, and indole were identified as efficient ligands for LTA4H. These fragments were elaborated in a small number of synthetic cycles into potent inhibitors of LTA4H representing multiple novel chemotypes for modulating leukotriene biosynthesis. Analysis of the fragment-bound structures also showed that the fragments comprehensively recapitulated key chemical features and binding modes of several reported LTA4H inhibitors.

Journal of Medicinal Chemistry, 2010

In Figure 2, the chemical structure of compound 19 (DG-051) was incorrectly drawn as the (R)-enan... more In Figure 2, the chemical structure of compound 19 (DG-051) was incorrectly drawn as the (R)-enantiomer. Compound 19 is the (S)-enantiomer and is properly shown in the three-dimensional crystal structure image at the righthand side in the corrected figure below. On page 4704, right hand column, first paragraph, line 19 and second paragraph line 22, (R)-prolinol should be changed to (S)-prolinol. Supporting Information Page S16. In Table 2, the chemical structure of compound 19 (DG-051) was incorrectly drawn as the (R)-enantiomer. The correct (S)-enantiomer has been included in the revised version of Supplemental Table 2 below.

Journal of Immunological Methods, 1995

The B9 assay is known to be a specific and sensitive assay for the estimation of interleukin-6 ac... more The B9 assay is known to be a specific and sensitive assay for the estimation of interleukin-6 activity. This assay was found to be compromised by lipopolysaccharide in concentrations > or = 40 ng lipopolysaccharide per ml. The lipopolysaccharide stimulates proliferation of the B9 cell line in a dose-dependent manner both when measuring the proliferation by thymidine incorporation and when using the MTT assay. However the LPS dose-response curve is different compared to the dose-response curve for IL-6. A sample containing 100 ng LPS/ml but no IL-6 would be estimated erroneously to contain 12 pg IL-6. The interference of lipopolysaccharide is totally abolished by the addition of polymyxin B to the samples but the addition has no effect on the IL-6 induced proliferation.

Journal of Hepatology, 1999

Background/Aims: Terlipressin is used for the treatment of bleeding oesophageal varices. We evalu... more Background/Aims: Terlipressin is used for the treatment of bleeding oesophageal varices. We evaluated the effects of terlipressin on hepatic haemodynamics, with special focus on the interactions between portal venous flow and hepatic arterial flow over time. Secondly, we evaluated the estimated hepatic blood flow by the ICG clearance method against direct measurements of hepatic blood flow. Methods: Eight healthy anaesthetised pigs received terlipressin 1 mg or placebo intravenously in a randomised, blind, cross-over design. Hepatic arterial flow, portal venous flow, systemic haemodynamics, and portal vein diameter were recorded simultaneously. Portal venous flow and hepatic arterial flow were measured by transit time ultrasound flowmetry. Estimated hepatic blood flows at baseline and after terlipressin were compared with the sum of the portal venous flow and hepatic arterial flow. Results: Portal venous flow decreased significantly 5 min after administration of terlipressin (p∞0.05). At 30 min it had decreased by 34% (p∞0.01) and the