Eriks Jakobsons - Academia.edu (original) (raw)

Papers by Eriks Jakobsons

Research paper thumbnail of Electrically active and 3D porous TiO2-x ceramic scaffolds for bone tissue regeneration

Journal of the European Ceramic Society, 2017

Research paper thumbnail of Follow-up of the patients after stem cell transplantation for pediatric dilated cardiomyopathy

Pediatric Transplantation, 2013

Dilated cardiomyopathy is a serious problem in pediatric cardiology. Despite the relatively low i... more Dilated cardiomyopathy is a serious problem in pediatric cardiology. Despite the relatively low incidence, the mortality is high. The conservative therapy does not improve the prognosis, and possibilities of heart transplantation are limited. There are multiple trials of use of stem cells for ischemic heart disease in the adult population. This allows us to believe that the method has perspectives in pediatric cardiology. We performed the cell therapy for seven patients, six of them had complete one yr follow-up after procedure. Five to 30 milliliters of bone marrow was aspirated from the iliac crest and 17 to 122 million BMCs were isolated. The average basal EF was 33.5%. We observed increasing of EF up to 54% (=9.54, p=0.00154) in a 6-month period and up to 54.5% (=10.82, p= 0.00315) after one yr. The changes of LVEDV also were observed. The LVEDV decreased in average per 13.05%. There were no observed side effects or heart rhythm disorders. Intramyocardial administration of bone marrow-derived progenitor cells proved to be a technically feasible and safe method. Up until now, the results obtained have been promising and we suppose that bone marrow-derived progenitor cell intramyocardial transplantation can be used.

Research paper thumbnail of First Results of using Stem Cell Transplantation for Pediatric Patients in Case of Dilated Cardiomyopathy

Acta Chirurgica Latviensis, 2010

Introduction. Dilated cardiomyopathy is a serious disorder of the myocardium in pediatric age. Co... more Introduction. Dilated cardiomyopathy is a serious disorder of the myocardium in pediatric age. Conservative therapy is limited and lethal outcome observed in one third of patients within a year. Bone marrow derived progenitor cell transplantation is becoming a promising method of treatment in adult population and there is ground to believe there are perspectives in pediatric cardiology. Aim of the Study. We present the first results of bone marrow cell transplantation in case of dilated cardiomyopathy for six patients at age four months to seventeen years. Materials and methods. We did the six Bone marrow derived progenitor cell (BMCs) intramycardial transplantations. Five to 30 mililiters of bone marrow were aspirated from iliac bone. Seventeen to 90 million BMCs were isolated and as suspension of physiologic saline given to patients by intramyocardial punction. Every patient underwent repeated examination every two month. Results. Six months following bone marrow derived progenitor stem cell intramyocardial transplantation we observed increase of ejection fraction in 4 patients, decrease of dilatation echocardiographycally and cardiothoracic ratio (CTR) at chest x-ray; decrease of the stage of heart insufficiency clinically from class IV to I-II(NYHA). Conclusions. We see the intramyocardial administration of bone marrow cells proved to be technically feasible and safe, also the procedure does not need for expensive technical equipment. The bone marrow transplantation improves the patients clinical situation and physical measurements. Our method might be used for the stabilization of the patient to get the time for further treatment.

Research paper thumbnail of The use of thermal analysis in assessing the effect of bound water content and substrate rigidity on prevention of platelet adhesion

Journal of Thermal Analysis and Calorimetry, 2014

ABSTRACT Implant surface-induced initiation of blood coagulation leading to thrombus formation is... more ABSTRACT Implant surface-induced initiation of blood coagulation leading to thrombus formation is a major problem. It is an important challenge in biomedical research to enhance thromboresistance of polymer coatings of blood-contacting implants and devices by the reduction of platelet adhesion to their surfaces. Currently, the molecular mechanisms responsible for interfacial processes related to regulation of protein adsorption and platelet adhesion in antithrombotic coatings are not yet clearly understood. We studied the role of water binding energy in chitosan molecules using differential scanning calorimetry and thermogravimetric analysis and the role of chitosan coating rigidity using dynamic mechanical thermal analysis in the prevention of platelet adhesion. It was found that the presence of loosely bound water in chitosan coatings increases platelet adhesion. The high molecular weight (HMW) chitosan coating that contains only tightly bound water prevents platelet adhesion. The high molecular weight (HMW) chitosan coating is more rigid and has higher platelet adhesion compared with more soft LMW chitosan coatings. The degree of hydration of thromboresistant coatings is significant parameter that must be taken into account in the design of blood-contacting surfaces.

Research paper thumbnail of Bone marrow mononuclear cell separation yield in myocardium infarction, coronary disease and type 2 diabetes and dilated cardiomyopathy patient groups

Research paper thumbnail of Cartilage-derived extracellular matrix extract promotes chondrocytic phenotype in three-dimensional tissue culture

Artificial cells, nanomedicine, and biotechnology, Jan 24, 2015

Cell transplantation is a promising regenerative therapy for cartilage degeneration. However, obt... more Cell transplantation is a promising regenerative therapy for cartilage degeneration. However, obtaining sufficient numbers of cells for this purpose is a challenge, due a lack of autologous donor tissue and the difficulty of culturing chondrocytes in vitro. Tissue engineering strategies that induce or maintain chondrocytic phenotype may solve these problems by (1) broadening the range of available donor tissue, and (2) facilitating the expansion of these cells while controlling phenotypic drift. In this study, bone marrow-derived mesenchymal stem cells (MSCs) and cartilage-derived cells (CDCs) were cultured on composite hydrogels containing agarose and homogenized cartilage extracellular matrix (ECM). MSCs cultured on agarose-ECM scaffolds did not show significant signs of chondrogenic differentiation in the absence of additional cues. However, CDCs cultured on agarose-ECM scaffolds proliferated more rapidly than their ECM-free counterparts and MSCs, while retaining chondrocytic mor...

Research paper thumbnail of Electrically active and 3D porous TiO2-x ceramic scaffolds for bone tissue regeneration

Journal of the European Ceramic Society, 2017

Research paper thumbnail of Follow-up of the patients after stem cell transplantation for pediatric dilated cardiomyopathy

Pediatric Transplantation, 2013

Dilated cardiomyopathy is a serious problem in pediatric cardiology. Despite the relatively low i... more Dilated cardiomyopathy is a serious problem in pediatric cardiology. Despite the relatively low incidence, the mortality is high. The conservative therapy does not improve the prognosis, and possibilities of heart transplantation are limited. There are multiple trials of use of stem cells for ischemic heart disease in the adult population. This allows us to believe that the method has perspectives in pediatric cardiology. We performed the cell therapy for seven patients, six of them had complete one yr follow-up after procedure. Five to 30 milliliters of bone marrow was aspirated from the iliac crest and 17 to 122 million BMCs were isolated. The average basal EF was 33.5%. We observed increasing of EF up to 54% (=9.54, p=0.00154) in a 6-month period and up to 54.5% (=10.82, p= 0.00315) after one yr. The changes of LVEDV also were observed. The LVEDV decreased in average per 13.05%. There were no observed side effects or heart rhythm disorders. Intramyocardial administration of bone marrow-derived progenitor cells proved to be a technically feasible and safe method. Up until now, the results obtained have been promising and we suppose that bone marrow-derived progenitor cell intramyocardial transplantation can be used.

Research paper thumbnail of First Results of using Stem Cell Transplantation for Pediatric Patients in Case of Dilated Cardiomyopathy

Acta Chirurgica Latviensis, 2010

Introduction. Dilated cardiomyopathy is a serious disorder of the myocardium in pediatric age. Co... more Introduction. Dilated cardiomyopathy is a serious disorder of the myocardium in pediatric age. Conservative therapy is limited and lethal outcome observed in one third of patients within a year. Bone marrow derived progenitor cell transplantation is becoming a promising method of treatment in adult population and there is ground to believe there are perspectives in pediatric cardiology. Aim of the Study. We present the first results of bone marrow cell transplantation in case of dilated cardiomyopathy for six patients at age four months to seventeen years. Materials and methods. We did the six Bone marrow derived progenitor cell (BMCs) intramycardial transplantations. Five to 30 mililiters of bone marrow were aspirated from iliac bone. Seventeen to 90 million BMCs were isolated and as suspension of physiologic saline given to patients by intramyocardial punction. Every patient underwent repeated examination every two month. Results. Six months following bone marrow derived progenitor stem cell intramyocardial transplantation we observed increase of ejection fraction in 4 patients, decrease of dilatation echocardiographycally and cardiothoracic ratio (CTR) at chest x-ray; decrease of the stage of heart insufficiency clinically from class IV to I-II(NYHA). Conclusions. We see the intramyocardial administration of bone marrow cells proved to be technically feasible and safe, also the procedure does not need for expensive technical equipment. The bone marrow transplantation improves the patients clinical situation and physical measurements. Our method might be used for the stabilization of the patient to get the time for further treatment.

Research paper thumbnail of The use of thermal analysis in assessing the effect of bound water content and substrate rigidity on prevention of platelet adhesion

Journal of Thermal Analysis and Calorimetry, 2014

ABSTRACT Implant surface-induced initiation of blood coagulation leading to thrombus formation is... more ABSTRACT Implant surface-induced initiation of blood coagulation leading to thrombus formation is a major problem. It is an important challenge in biomedical research to enhance thromboresistance of polymer coatings of blood-contacting implants and devices by the reduction of platelet adhesion to their surfaces. Currently, the molecular mechanisms responsible for interfacial processes related to regulation of protein adsorption and platelet adhesion in antithrombotic coatings are not yet clearly understood. We studied the role of water binding energy in chitosan molecules using differential scanning calorimetry and thermogravimetric analysis and the role of chitosan coating rigidity using dynamic mechanical thermal analysis in the prevention of platelet adhesion. It was found that the presence of loosely bound water in chitosan coatings increases platelet adhesion. The high molecular weight (HMW) chitosan coating that contains only tightly bound water prevents platelet adhesion. The high molecular weight (HMW) chitosan coating is more rigid and has higher platelet adhesion compared with more soft LMW chitosan coatings. The degree of hydration of thromboresistant coatings is significant parameter that must be taken into account in the design of blood-contacting surfaces.

Research paper thumbnail of Bone marrow mononuclear cell separation yield in myocardium infarction, coronary disease and type 2 diabetes and dilated cardiomyopathy patient groups

Research paper thumbnail of Cartilage-derived extracellular matrix extract promotes chondrocytic phenotype in three-dimensional tissue culture

Artificial cells, nanomedicine, and biotechnology, Jan 24, 2015

Cell transplantation is a promising regenerative therapy for cartilage degeneration. However, obt... more Cell transplantation is a promising regenerative therapy for cartilage degeneration. However, obtaining sufficient numbers of cells for this purpose is a challenge, due a lack of autologous donor tissue and the difficulty of culturing chondrocytes in vitro. Tissue engineering strategies that induce or maintain chondrocytic phenotype may solve these problems by (1) broadening the range of available donor tissue, and (2) facilitating the expansion of these cells while controlling phenotypic drift. In this study, bone marrow-derived mesenchymal stem cells (MSCs) and cartilage-derived cells (CDCs) were cultured on composite hydrogels containing agarose and homogenized cartilage extracellular matrix (ECM). MSCs cultured on agarose-ECM scaffolds did not show significant signs of chondrogenic differentiation in the absence of additional cues. However, CDCs cultured on agarose-ECM scaffolds proliferated more rapidly than their ECM-free counterparts and MSCs, while retaining chondrocytic mor...