Ernesto Carvalho - Academia.edu (original) (raw)

Papers by Ernesto Carvalho

Phytotherapy Research, 2011

The present study isolated three major active flavonoids, two flavones named 4’,5,7‐trimethoxy‐lu... more The present study isolated three major active flavonoids, two flavones named 4’,5,7‐trimethoxy‐luteolin (1) and 6‐hydroxy‐5,7‐dimethoxyflavone (2) and the flavanone 5‐hydroxy‐6,7‐dimethoxyflavanone (3) from Zeyheria montana dichloromethane leaf extract. Isolation and purification were conducted with the application of column chromatography and structures were assigned by spectral analysis. All compounds were evaluated for cytotoxic activities against human tumor cell lines UACC‐62 (melanoma), MCF‐7 (breast), NCI‐ADR/RES (breast expressing phenotype multiple drug resistance), 786‐0 (renal), NCI‐H460 (lung, non‐small cells), PC‐3 (prostate), OVCAR‐3 (ovarian), HT‐29 (colon) and K562 (leukemia) in vitro. All compounds were active in different degrees on several tumor cell lines and flavanone 3 showed cytotoxicity against almost all cell lines, particularly against human NCI‐ADR/RES and K562 cell lines. In conclusion, three antiproliferative compounds were isolated for the first time fr...

Brazilian Journal of Health Review

Síndrome de Brugada (SBr) é uma condição arrítmica não estrutural caracterizada por anormalidades... more Síndrome de Brugada (SBr) é uma condição arrítmica não estrutural caracterizada por anormalidades eletrocardiográficas do segmento ST nas derivações precordiais direita, associada a risco elevado de arritmias ventriculares e morte súbita. O cardioversor desfibrilador implantável é a melhor conduta para prevenção desses eventos fatais, sendo a síndrome mais prevalente em homens do que em mulheres (proporção de 8-9:1). Identificar o quantitativo de artigos publicados que abordam sobre os aspectos fisiopatológicos, os métodos atuais no diagnóstico e como é realizado o tratamento dessa doença. Pesquisa desenvolvida através da revisão sistemática da literatura do quantitativo de estudo publicado sobre a fisiologia, fisiopatologia, diagnóstico e tratamento dessa síndrome. O referencial teórico foi baseado na análise de artigos publicados nos últimos cinco anos nas bases científicas Scielo, Medline e Lilacs, foram encontrados 402 artigos, sendo 31 selecionados para a escrita deste trabalho...

Acta Neurochirurgica, 2019

Brain biopsy has a well-established role in the diagnosis of CNS neoplasia. Nevertheless, despite... more Brain biopsy has a well-established role in the diagnosis of CNS neoplasia. Nevertheless, despite being essential for the diagnosis of some benign neurological diseases, little consensus exists regarding its indications for disease diagnosis and patient orientation. Our aim was to assess brain biopsy diagnostic yield in patients with neurological deterioration of unknown etiology, to identify the clinical characteristics associated with an increased likelihood of achieving a diagnostic biopsy as well as the characteristics linked to a particular diagnosis. Methods A retrospective analysis of 62 consecutive brain biopsies performed at a single tertiary care center between January 2004 and December 2015 for suspected non-neoplastic neurological disease was performed. The clinical presentation, imaging, and laboratory results were collected and compared between diagnostic groups. Results Sixty-eight percent of the biopsies led to a definitive diagnosis. The most common histological diagnosis was central nervous system lymphoma (eight cases), followed by astrocytoma, demyelinating disease, and progressive multifocal leukoencephalopathy (four cases each). No clinical characteristics were found to predict a diagnostic biopsy or to correlate with a specific diagnosis. Importantly, a distinct diagnosis from the initially suspected was achieved in 52% of cases and biopsy findings led to a change of therapeutic orientation in 78% of the cases. Conclusions Our results suggest that brain biopsies have a significant impact on patient management and should be considered early in selected cases in which less invasive testing was unable to reach a definitive diagnosis.

Arquivos Brasileiros de Neurocirurgia: Brazilian Neurosurgery, 2018

The vestibular schwannoma is the most common extra-axial tumor of the posterior fossa. Hemorrhage... more The vestibular schwannoma is the most common extra-axial tumor of the posterior fossa. Hemorrhage associated with vestibular schwannomas has been described in less than 1% of the cases. The etiology remains unknown; however, some risk factors have been suggested, such as tumor size and tumor growth rate, the vascularity and histopathology of the tumor or even previous treatment with radiosurgery. The present case is of a 74-year-old female patient, who presented to our clinic in December of 2015 after a mild traumatic brain injury. In that context, she did a brain computed tomography (CT) scan and was diagnosed with a vestibular schwannoma— an incidentaloma. It was decided at that time to treat the patient conservatively because of her comorbidities and the presentation of the disease. In March of 2017, the patient presented again to our clinic with a right peripheral facial paralysis (House-Brackmann [HB] grade IV-VI) and confusion. The CT scan revealed that bleeding around the ves...

Arquivos Brasileiros de Neurocirurgia: Brazilian Neurosurgery, 2014

ResumoA derivação ventriculoperitoneal é atualmente o método mais utilizado no tratamento da hidr... more ResumoA derivação ventriculoperitoneal é atualmente o método mais utilizado no tratamento da hidrocefalia comunicante. As complicações mais comuns são a disfunção do shunt e infecções. Este relato de caso tem como objetivo apresentar um doente com idas recorrentes ao serviço de urgência por agitação psicomotora e dor abdominal, e cujo cateter distal previamente desconectado encontrava-se alojado no interior do saco escrotal através de hérnia inguinoescrotal. A formação de hérnia inguinal com migração de cateter é um evento raro no adulto, sendo mais comum na criança, possivelmente pela maior taxa de patência do processo vaginal e aumento da pressão intra-abdominal. No momento em que este artigo foi redigido, não foi encontrado caso semelhante na literatura.

The International journal of neuroscience, Jan 2, 2017

Supratentorial hemangioblastoma is an uncommon lesion with few data available. Resemblance to oth... more Supratentorial hemangioblastoma is an uncommon lesion with few data available. Resemblance to other tumours represents a struggle concerning preoperative diagnosis and management. The aim of this study was to review the current literature, integrating two new cases with uncommon features. A search of English language peer-reviewed articles in PubMed®, Cochrane Library®, Google Scholar®, and summary of the current knowledge. A total of 162 cases, with no predominance between von Hippel Lindau disease and wild-type sporadic lesions. Usual topographic presentation on frontal lobe or pituitary stalk, with imagological resemblance to a glioma or a metastasis. From these, 20 cases revealed dural attachment, mimicking a meningioma. Symptoms are due to mass effect or epilepsy, with low haemorrhagic risk. Clinical outcome on supratentorial hemangioblastoma depends on resection extension, with no recognised complementary treatment. Postoperative follow-up is essential, even in wild-type, spor...

Journal of Spine, 2017

Sclerosing epithelioid fibrosarcoma (SEF) is a singular entity, recognized as malignant fibroblas... more Sclerosing epithelioid fibrosarcoma (SEF) is a singular entity, recognized as malignant fibroblastic sarcoma variant, which deviates from classic type due to the indolent progression and late metastasis. Typically occurring in middle aged adults, it presents a predilection for deep soft tissues, particularly skeletal muscle. It often involves the fascia or periosteum, and less frequently it may invade or arise from bone. Diagnosis and recognition are critical, as the generally bland appearance or when intraosseous similarity with an osteoid lesion, can lead to misdiagnosis. We herein present a case showing an unusual location and challenging radiological morphological and surgical features.

Arquivos Brasileiros de Neurocirurgia: Brazilian Neurosurgery, 2017

Objective The treatment of ruptured aneurysms of the posterior circulation is a controversy in ne... more Objective The treatment of ruptured aneurysms of the posterior circulation is a controversy in neurosurgery. The aim of this work is to describe the experience and results of the early surgical treatment of this pathology at Centro Hospitalar do Porto. Method We retrospectively analyzed the medical records of all patients aged over 18 who, in the period between 1999–2013, were admitted to our center with the diagnosis of ruptured saccular posterior circulation aneurysm. The patients were clinically staged at admission using the Hunt & Hess (H&H) scale. The modified Glasgow Outcome Scale (mGOS) was used to assess the outcome at discharge and after 6 months. Results Between 1999–2013, 59 patients underwent surgery for ruptured posterior circulation aneurysms. Eighty percent of the patients were female, and their average age was 58.7 years. Posterior-inferior cerebellar artery aneurysms accounted for 49.2% of surgeries, while basilar aneurysms accounted for 28.8%. Upon admission, 86.4%...

Arquivos Brasileiros de Neurocirurgia: Brazilian Neurosurgery, 2016

A fifty-five years-old patient with complaints of neck and shoulder pain for a week, without refe... more A fifty-five years-old patient with complaints of neck and shoulder pain for a week, without reference to a triggering factor, namely exercise or a traumatic event. The symptoms had increased in the 24 hours previous to the hospital observation, without any relief with immobilization or postural correction. Four hours after pain exacerbation, he described motor deficit of right side limbs, without other neurological disorder.

Cancer Epidemiology Biomarkers & Prevention, 2011

Background: The epidermal growth factor receptor (EGFR) regulates important cellular processes an... more Background: The epidermal growth factor receptor (EGFR) regulates important cellular processes and is frequently implicated in human tumors. Three EGFR polymorphisms have been described as having a transcriptional regulatory function: two single-nucleotide polymorphisms in the essential promoter region, À216G/T and À191C/A, and a polymorphic (CA) n microsatellite sequence in intron 1. We aimed to elucidate the roles of these EGFR polymorphisms in glioma susceptibility and prognosis. Methods: We conducted a case-control study with 196 patients with glioma and 168 cancer-free controls. Unconditional multivariate logistic regression models were used to calculate ORs and 95% confidence intervals. A Cox regression model was used to evaluate associations with patient survival. False-positive report probabilities were also assessed. Results: None of the EGFR À216G/T variants was significantly associated with glioma risk. The À191C/A genotype was associated with higher risk for glioma when the (CA) n alleles were classified as short for 16 or 17 repeats. Independently of the (CA) n repeat cutoff point used, shorter (CA) n repeat variants were significantly associated with increased risk for glioma, particularly glioblastoma and oligodendroglioma. In all tested models with different (CA) n cutoff points, only À191C/A genotype was consistently associated with improved survival of patients with glioblastoma. Conclusions: Our findings implicate EGFR À191C/A and the (CA) n repeat polymorphisms as risk factors for gliomas, and suggest À191C/A as a prognostic marker in glioblastoma. Impact: Our data support a role of these EGFR polymorphisms in determining glioma susceptibility, with potential relevance for molecularly based stratification of patients with glioblastoma for individualized therapies. Cancer Epidemiol Biomarkers Prev; Ó2011 AACR.

Networking and …, 1999

... University of Fortaleza - UNIFOR, DsC, BRESIL (3) ***Computer Science Department, Federal Un... more ... University of Fortaleza - UNIFOR, DsC, BRESIL (3) ***Computer Science Department, Federal University of Ceará, DsC, BRESIL Résumé / Abstract. Due to the needs and flexibility of current computer networks, more efficient and flexible management systems must be in ...

Natural Product Research, 2012

The essential oils from the leaves of Annona pickelii and Annona salzmannii (Annonaceae) were obt... more The essential oils from the leaves of Annona pickelii and Annona salzmannii (Annonaceae) were obtained by hydrodistillation using a Clevenger apparatus, and analysed by GC-MS and GC-FID. A total of 21 compounds were identified in the essential oil of A. pickelii and 23 in that of A. salzmannii; sesquiterpenes predominated in both essential oils. Bicyclogermacrene (38.0%), (E)-caryophyllene (27.8%), α-copaene (6.9%)