Esra Çikler - Academia.edu (original) (raw)
Papers by Esra Çikler
TÜRKÇE TEZ BAŞLIKSudan Kaçınma Stresi Uygulanan Sıçanlarda Mesane Epiteli Üzerine Lökotrien Resep... more TÜRKÇE TEZ BAŞLIKSudan Kaçınma Stresi Uygulanan Sıçanlarda Mesane Epiteli Üzerine Lökotrien Reseptör Antagonisti Montelukastın Etkisinin Araştırılması Adı ve Soyadı : Esra ÇİKLER Danışman Adı : Şule Çetinel Kabul Tarihi : 24.8.2004 Program : Yüksek Lisans Anabilim Dalı : Histoloji ve EmbriyolojiFiziksel ve psikolojik stres türlerinin mesane epiteli üzerinde olumsuz etkileri vardır. Sebebi tam olarak anlaşılamamış olan interstisiyel sistit (İS)' in stres koşullarından etkilendiği düşünülmektedir.Bu çalışmanın amacı:Kronik olarak uygulanan sudan kaçınma stresi ile oluşturulan mesane hasarı üzerine lökotrien D4 (LTD4) reseptör antagonisti montelukastın etkisinin morfolojik ve biyokimyasal olarak araştırılması, stres sonucunda artan degranüle mast hücre sayı ve morfolojisi üzerine montelukastın etkisinin gösterilmesidir.Wistar Albino dişi sıçanlara 5 gün, 2 saat boyunca sudan kaçınma stresi (SKS) uygulanmıştır. Tedavi gruplarına her stres uygulamasından sonra intraperitoneal (i.p.) ...
ÖZETOrganizmalar yaşamlarını dış çevreleriyle dinamik bir denge içinde sürdürürler. Stres bu norm... more ÖZETOrganizmalar yaşamlarını dış çevreleriyle dinamik bir denge içinde sürdürürler. Stres bu normal homeostazı bozar ve pek çok hastalığın patogenezinde etkili olur. Şiddetli ya da aralıklı olarak uygulanan kronik stres modelleri hayvanların üreme yetenekleri üzerinde olumsuz etkiye sahiptirler.Bu çalışmanın amacı, iki farklı fizyolojik ve psikolojik stres modeli olan kronik sudan kaçınma ve hareketsizlik streslerinin ardışık uygulanmasıyla Sertoli hücrelerinde oluşturulan hasarın immünhistokimyasal olarak gösterilmesidir. Çalışmamızda iki deney grubu kullanılmıştır: 1) Kontrol grup (K), 2) Stres grup (KS). Kronik stres grubundaki Wistar Albino erkek sıçanlara 3 gün 2şer saat sudan kaçınma stresi (SKS), bunu takip eden 2 gün 2şer saat hareketsizlik stresi (HS) uygulanmıştır. Deneylerin sonunda testis örneklerine histolojik incelemeler için hematoksilen-eozin ve Masson’un trikrom boyaları ile β-aktin immünhistokimyası uygulanmıştır. Sertoli hücrelerindeki aktin dağılımının ince-yapıs...
A total of 45 male Sprague-Dawley rats were separated into 5 groups. PUO was created in all rats ... more A total of 45 male Sprague-Dawley rats were separated into 5 groups. PUO was created in all rats except the control group (1). At the 6th week after PUO, 50 μl of saline (2 sham groups) or 50 μl BONT-A (2 treatment groups) was injected in 5 divided doses of total 50 U (50 μl) in the anterior, posterior, left and right lateral walls and dome of the bladder (2). Treatment and sham groups were studied 5 and 30 days after injection and neuro-pharmacological and histo-morphological findings on bladder tissues were compared to the control group (figure1).
The Journal of Spinal Cord Medicine, 2010
Background: Oxidative stress is a mediator of secondary injury to the spinal cord following traum... more Background: Oxidative stress is a mediator of secondary injury to the spinal cord following trauma. Objective: To investigate the putative neuroprotective effect of a-lipoic acid (LA), a powerful antioxidant, in a rat model of spinal cord injury (SCI). Methods: Wistar albino rats were divided as control, vehicle-treated SCI, and LA-treated SCI groups. To induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10 was used. Injured animals were given either 50 mg/kg LA or saline at 30 minutes postinjury by intraperitoneal injection. At 7 days postinjury, neurologic examination was performed, and rats were decapitated. Spinal cord samples were taken for histologic examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, and DNA fragmentation. Formation of reactive oxygen species in spinal cord tissue samples was monitored by using a chemiluminescence (CL) technique. Results: SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in luminol CL and MDA levels, MPO activity, and DNA damage. Furthermore, LA treatment reversed all these biochemical parameters as well as SCI-induced histopathologic alterations. Conversely, impairment of the neurologic function caused by SCI remained unchanged. Conclusion: The present study suggests that LA reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, glutathione depletion, and DNA fragmentation.
Marmara Medical Journal
ABSTRACT Objective: The aim of the present study was to investigate the effects of aqueaous garli... more ABSTRACT Objective: The aim of the present study was to investigate the effects of aqueaous garlic extract (AGE) on water avoidance stress (WAS)-induced degranulation of mast cells in the dermis. Material and Methods: Wistar albino rats were exposed to WAS (WAS group). After exposing the animals to WAS (WAS plus AGE group), 250mg/kg AGE was injected i.p.. Dermal mast cells were stained with toluidine blue and investigated using light microscopy. Results: The number of both granulated and degranulated mast cells was higher in theWAS group, when compared to the control group. The number of mature granulated and degranulated mast cells was lower in the WAS plus AGE group when compared to the WAS group. Conclusion: Chronic AGE treatment reduced WAS-induced infiltration and activation of mast cells in the dermis and may provide a useful therapeutic option in stress-induced skin disorders. Keywords: Dermis, Mast cell, Water avoidence stress, Aqueaus garlic extract
Plant Foods for Human Nutrition, 2005
Urologia Internationalis, 2006
a critical role in this injury. The signifi cant decrease in MDA and increase in glutathione leve... more a critical role in this injury. The signifi cant decrease in MDA and increase in glutathione levels in the PS + AGE group was in accordance with morphological fi ndings. Based on the results, AGE treatment signifi cantly prevented PS-induced degenerative morpho logical and biochemical changes of urinary bladder mucosa.
European Urology Supplements, 2004
Marmara …, 2005
Objective: This morphological and biochemical study aims to investigate the putative protective e... more Objective: This morphological and biochemical study aims to investigate the putative protective effects of 2-mercaptoethane sulfonate (MESNA) on protamine sulfate (PS) induced bladder injury. Material and methods: Wistar albino female rats were ...
World Journal of Urology, 2006
The present study was designed to investigate the putative protective effects of taurine on prota... more The present study was designed to investigate the putative protective effects of taurine on protamine sulfate (PS) induced bladder injury. Wistar albino female rats were catheterized and intravesically infused with phosphate buffered solution (control group) or PS (PS group) dissolved in phosphate buffered solution. In the PS + taurine (PS+Tau) group, after the PS instillation, taurine (50 mg/kg) was injected intraperitoneally for 3 days. Histopathological changes were investigated by light and scanning electron microscopy. Tissue samples were also obtained to determine bladder malondialdehyde (MDA) (a biomarker of oxidative damage) and glutathione (GSH) (a biomarker of protective oxidative injury) levels. In the PS group ulcerated areas, an irregular mucus layer, inflammatory cell infiltration, and increased number of mast cells were observed. In the PS+Tau group, a relatively normal urothelial topography, glycosaminoglycan layer, and decreased number of mucosal mast cells and inflammatory cells were observed. Increased MDA levels as a result of PS induction lead us to propose that free radicals may have a critical role in this injury. The significant decrease in MDA and increase in GSH levels in the PS+Tau group compared to PS group was in accordance with morphological findings. Based on the results, taurine treatment significantly prevented PS induced degenerative morphological and biochemical changes of urinary bladder mucosa.
Pharmacology, 2005
Several studies demonstrate that taurine treatment prevents tissue damage in various models of in... more Several studies demonstrate that taurine treatment prevents tissue damage in various models of inflammation. Experiments have shown that chronic nicotine administration caused oxidant damage in various organs by increasing lipid peroxidation products and decreasing the activity of endogenous antioxidants. The aim of this study was to investigate the effects of taurine treatment on nicotine-induced oxidative changes in rat urinary bladder and kidney and to explore the possible mechanisms of action. Male Wistar albino rats were injected with nicotine hydrogen bitartrate (0.6 mg/kg i.p.) or saline for 21 days. Taurine was administered (50 mg/kg i.p.) alone or along with nicotine injections. At the end of the treatment period bladder tissue was used for in vitro contractility studies, or stored along with kidney tissue for the measurement of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen content. Tissue samples were also examined histolog...
Journal of Urology, 2005
The role of melatonin on chronic water avoidance stress (WAS) induced degeneration of bladder epi... more The role of melatonin on chronic water avoidance stress (WAS) induced degeneration of bladder epithelium (urothelium) was investigated. Wistar albino rats were exposed to WAS for 2 hours daily for 5 days (WAS group). Before exposing them to WAS 10 mg/kg melatonin (WAS plus melatonin group) was injected intraperitoneally into the animals. Bladder samples were investigated with light and electron microscopy. Lipid peroxidation and glutathione levels were also measured in the bladder. In the WAS only group an increased number of mast cells in the mucosa, ulcerated areas, vacuole formation and dilated perinuclear cisternae, and dilatation in the intercellular spaces in the urothelium were observed. In the WAS plus melatonin group relatively normal urothelial topography, a decreased number of mast cells in the mucosa, some dilatation between intercellular areas, regular perinuclear cisternae and tight junctions were observed. Increased lipid peroxidation and decreased glutathione levels in WAS rats were reversed by melatonin. The results show that melatonin reverses WAS induced degenerative changes in the bladder.
Journal of Medicinal Food, 2005
Fundamental and Clinical Pharmacology, 2005
Nicotine, a major toxic component of cigarette smoking, has long been recognized to result in oxi... more Nicotine, a major toxic component of cigarette smoking, has long been recognized to result in oxidative stress by inducing the generation of reactive oxygen species in the periphery, and central nervous system. Experiments have shown that chronic administration of nicotine caused increased lipid peroxidation products in serum and tissues of rats [1,2]. The increased concentration of lipid peroxidation products observed in nicotine treated rats was found to be associated with decreased activity of scavenging enzymes such as catalase and superoxide dismutase [2]. A decrease in the activities of these enzymes can lead to the excessive availability of superoxides and peroxyl radicals, which in turn generate hydroxyl radicals, resulting in the initiation and propagation of lipid peroxidation. Lipid peroxidation is known to cause cellular injury by inactivation of membrane enzymes and receptors, depolymerization of polysaccharide, as well as protein cross-linking and fragmentation. Since membrane lipids are vital for the maintenance and integrity of cell function, the breakdown of membrane phospholipids and lipid peroxidation are expected to change membrane structure, fluidity, transport and
Digestive Diseases and Sciences, 2006
We investigated the role of taurine, is a potent free radical scavenger, on water avoidance stres... more We investigated the role of taurine, is a potent free radical scavenger, on water avoidance stress (WAS)-induced degeneration of the gastric, ileal, and colonic mucosa and liver parenchyma. Wistar albino rats were exposed to chronic WAS (WAS group) 2 hr daily for 5 days. After exposing animals to chronic WAS (WAS + taurine group), 50 mg/kg taurine was injected IP for 3 days. Control animals received vehicle solution only. The stomach, ileum, colon, and liver samples were investigated under light microscope for histopathologic changes. To demonstrate the topography of the luminal mucosa of the stomach, ileum, and colon, scanning electron microscope was used and for hepatocyte ultastructure transmission electron microscope was used. Malondialdehyde (MDA, a biomarker of oxidative damage) and glutathione (GSH, a biomarker of protective oxidative injury) levels were also determined in all tissues. In the WAS group, the stomach epithelium showed ulceration in some areas, dilatations of the gastric glands, and degeneration of gastric glandular cells; prominent congestion of the capillaries was apparent. In the WAS group, severe vascular congestion was observed along with degeneration of ileal and colonic epithelium. Prominent vascular congestion and dilated sinusoids, activated Kupffer cells, dilated granular endoplasmic reticulum membranes, and focal pyknotic nuclei were observed in liver parenchyma. MDA levels (stomach, P < 0.01; ileum, colon, and liver P < 0.05) were increased and GSH levels (P < 0.01) were decreased in all tissues in the WAS group compared with the control group. The morphology of gastric, ileal, and colonic mucosa and liver parenchyma in the WAS + taurine group (stomach and ileum, P < 0.05; colon and liver, P < 0.01) showed a significant amelioration when compared to the WAS group. Increased MDA and decreased GSH levels in the WAS group were ameliorated with taurine treatment. Based on the results, taurine supplementation effectively attenuates the oxidative damage of gastrointestinal mucosa and liver because of WAS induction possibly by its antioxidant effects.
Digestive Diseases and Sciences, 2007
We investigated the effect of aqueous garlic extract (AGE) on water avoidance stress (WAS)-induce... more We investigated the effect of aqueous garlic extract (AGE) on water avoidance stress (WAS)-induced degeneration of the gastric and ileal mucosa and liver parenchyma. Wistar albino rats were exposed to WAS (WAS group) for 5 days. After exposure of the animals to WAS, a 1 ml/kg aqueous garlic extract (AGE) was injected i.p. (WAS+AGE group). The stomach, ileum, and liver samples were investigated under light microscope for general morphology. Topography of gastric and ileal mucosa was investigated by scanning electron microscopy, and hepatocyte ultastructure by transmission electron micsroscopy. Malondialdehyde (MDA) and glutathione (GSH) levels of all tissues were also determined. In the WAS group, the epithelium of the stomach showed ulceration in some areas, dilatations of the gastric glands, and degeneration of gastric glandular cells. Severe vascular congestion and degeneration of ileal epithelium were observed. Prominent vascular congestion and dilated sinusoids, activated Kupffer cells with prominent morphology, dilated granular endoplasmic reticulum membranes, and focal picnotic nuclei were observed in liver parenchyma. AGE treatment reduced the degeneration of the gastric and ileal mucosa and liver parenchyma. Increased MDA levels and decreased GSH levels in the WAS group were reversed to control values after AGE treatment. Based on these results, AGE treatment significantly prevented WAS-induced degeneration in both morphology and biochemistry of gastrointestinal mucosa and liver parenchyma due to its potent free radical scavenging and antioxidant properties.
Digestive Diseases and Sciences, 2005
mmj.dergisi.org
... 2-MERCAPTOETAN SÜLFONAT (MESNA)'IN, PROTAMİN SÜLFATIN TETİKLEDİĞİ MESANE HAS... more ... 2-MERCAPTOETAN SÜLFONAT (MESNA)'IN, PROTAMİN SÜLFATIN TETİKLEDİĞİ MESANE HASARI ÜZERİNDEKİ KORUYUCU ETKİLERİ. Beyhan Sağlam 1 , Esra Cikler 1 , Ali Zeybek 2 , Şule Çetinel 1 , Feriha Ercan 1 , Göksel Şener 3. ...
BJU International, 2006
To investigate the role of aqueous garlic extract (AGE) on the water-avoidance stress (WAS)-induc... more To investigate the role of aqueous garlic extract (AGE) on the water-avoidance stress (WAS)-induced degeneration of the urinary bladder in a rat model. Wistar albino rats were exposed to WAS for 2 h/day for 5 days (WAS group), after which, AGE (1 mL/kg) was injected intraperitoneally into the rats (WAS + AGE group). Urinary bladder samples were investigated with both light and scanning electron microscopy, and lipid peroxidation and glutathione levels were also measured in the samples. In the WAS group there was inflammatory cell infiltration, more mast cells and ulcerated areas in the mucosa. In the WAS + AGE group there was relatively normal urothelial alignment, moderate inflammatory cell infiltration and fewer mast cells in the mucosa. The increased lipid peroxidation and decreased glutathione levels in WAS rats were reversed by AGE treatment. These results show that AGE has a protective effect on WAS-induced degenerative changes in the urinary bladder.
mmj.dergisi.org
... 4) Huston DP, Bressler RB. Urticaria and angioedema. Med Clin North Am 1992; 76:805840. 5) O... more ... 4) Huston DP, Bressler RB. Urticaria and angioedema. Med Clin North Am 1992; 76:805840. 5) Ozdamar SO, Seckin D, Kandemir B, Turanli AY. Mast cells in psoriasis. Dermatology 1996; 192:189190. 6) Farber EM, Nickoloff BJ, Recht B, Fraki JE. ...
TÜRKÇE TEZ BAŞLIKSudan Kaçınma Stresi Uygulanan Sıçanlarda Mesane Epiteli Üzerine Lökotrien Resep... more TÜRKÇE TEZ BAŞLIKSudan Kaçınma Stresi Uygulanan Sıçanlarda Mesane Epiteli Üzerine Lökotrien Reseptör Antagonisti Montelukastın Etkisinin Araştırılması Adı ve Soyadı : Esra ÇİKLER Danışman Adı : Şule Çetinel Kabul Tarihi : 24.8.2004 Program : Yüksek Lisans Anabilim Dalı : Histoloji ve EmbriyolojiFiziksel ve psikolojik stres türlerinin mesane epiteli üzerinde olumsuz etkileri vardır. Sebebi tam olarak anlaşılamamış olan interstisiyel sistit (İS)' in stres koşullarından etkilendiği düşünülmektedir.Bu çalışmanın amacı:Kronik olarak uygulanan sudan kaçınma stresi ile oluşturulan mesane hasarı üzerine lökotrien D4 (LTD4) reseptör antagonisti montelukastın etkisinin morfolojik ve biyokimyasal olarak araştırılması, stres sonucunda artan degranüle mast hücre sayı ve morfolojisi üzerine montelukastın etkisinin gösterilmesidir.Wistar Albino dişi sıçanlara 5 gün, 2 saat boyunca sudan kaçınma stresi (SKS) uygulanmıştır. Tedavi gruplarına her stres uygulamasından sonra intraperitoneal (i.p.) ...
ÖZETOrganizmalar yaşamlarını dış çevreleriyle dinamik bir denge içinde sürdürürler. Stres bu norm... more ÖZETOrganizmalar yaşamlarını dış çevreleriyle dinamik bir denge içinde sürdürürler. Stres bu normal homeostazı bozar ve pek çok hastalığın patogenezinde etkili olur. Şiddetli ya da aralıklı olarak uygulanan kronik stres modelleri hayvanların üreme yetenekleri üzerinde olumsuz etkiye sahiptirler.Bu çalışmanın amacı, iki farklı fizyolojik ve psikolojik stres modeli olan kronik sudan kaçınma ve hareketsizlik streslerinin ardışık uygulanmasıyla Sertoli hücrelerinde oluşturulan hasarın immünhistokimyasal olarak gösterilmesidir. Çalışmamızda iki deney grubu kullanılmıştır: 1) Kontrol grup (K), 2) Stres grup (KS). Kronik stres grubundaki Wistar Albino erkek sıçanlara 3 gün 2şer saat sudan kaçınma stresi (SKS), bunu takip eden 2 gün 2şer saat hareketsizlik stresi (HS) uygulanmıştır. Deneylerin sonunda testis örneklerine histolojik incelemeler için hematoksilen-eozin ve Masson’un trikrom boyaları ile β-aktin immünhistokimyası uygulanmıştır. Sertoli hücrelerindeki aktin dağılımının ince-yapıs...
A total of 45 male Sprague-Dawley rats were separated into 5 groups. PUO was created in all rats ... more A total of 45 male Sprague-Dawley rats were separated into 5 groups. PUO was created in all rats except the control group (1). At the 6th week after PUO, 50 μl of saline (2 sham groups) or 50 μl BONT-A (2 treatment groups) was injected in 5 divided doses of total 50 U (50 μl) in the anterior, posterior, left and right lateral walls and dome of the bladder (2). Treatment and sham groups were studied 5 and 30 days after injection and neuro-pharmacological and histo-morphological findings on bladder tissues were compared to the control group (figure1).
The Journal of Spinal Cord Medicine, 2010
Background: Oxidative stress is a mediator of secondary injury to the spinal cord following traum... more Background: Oxidative stress is a mediator of secondary injury to the spinal cord following trauma. Objective: To investigate the putative neuroprotective effect of a-lipoic acid (LA), a powerful antioxidant, in a rat model of spinal cord injury (SCI). Methods: Wistar albino rats were divided as control, vehicle-treated SCI, and LA-treated SCI groups. To induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10 was used. Injured animals were given either 50 mg/kg LA or saline at 30 minutes postinjury by intraperitoneal injection. At 7 days postinjury, neurologic examination was performed, and rats were decapitated. Spinal cord samples were taken for histologic examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, and DNA fragmentation. Formation of reactive oxygen species in spinal cord tissue samples was monitored by using a chemiluminescence (CL) technique. Results: SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in luminol CL and MDA levels, MPO activity, and DNA damage. Furthermore, LA treatment reversed all these biochemical parameters as well as SCI-induced histopathologic alterations. Conversely, impairment of the neurologic function caused by SCI remained unchanged. Conclusion: The present study suggests that LA reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, glutathione depletion, and DNA fragmentation.
Marmara Medical Journal
ABSTRACT Objective: The aim of the present study was to investigate the effects of aqueaous garli... more ABSTRACT Objective: The aim of the present study was to investigate the effects of aqueaous garlic extract (AGE) on water avoidance stress (WAS)-induced degranulation of mast cells in the dermis. Material and Methods: Wistar albino rats were exposed to WAS (WAS group). After exposing the animals to WAS (WAS plus AGE group), 250mg/kg AGE was injected i.p.. Dermal mast cells were stained with toluidine blue and investigated using light microscopy. Results: The number of both granulated and degranulated mast cells was higher in theWAS group, when compared to the control group. The number of mature granulated and degranulated mast cells was lower in the WAS plus AGE group when compared to the WAS group. Conclusion: Chronic AGE treatment reduced WAS-induced infiltration and activation of mast cells in the dermis and may provide a useful therapeutic option in stress-induced skin disorders. Keywords: Dermis, Mast cell, Water avoidence stress, Aqueaus garlic extract
Plant Foods for Human Nutrition, 2005
Urologia Internationalis, 2006
a critical role in this injury. The signifi cant decrease in MDA and increase in glutathione leve... more a critical role in this injury. The signifi cant decrease in MDA and increase in glutathione levels in the PS + AGE group was in accordance with morphological fi ndings. Based on the results, AGE treatment signifi cantly prevented PS-induced degenerative morpho logical and biochemical changes of urinary bladder mucosa.
European Urology Supplements, 2004
Marmara …, 2005
Objective: This morphological and biochemical study aims to investigate the putative protective e... more Objective: This morphological and biochemical study aims to investigate the putative protective effects of 2-mercaptoethane sulfonate (MESNA) on protamine sulfate (PS) induced bladder injury. Material and methods: Wistar albino female rats were ...
World Journal of Urology, 2006
The present study was designed to investigate the putative protective effects of taurine on prota... more The present study was designed to investigate the putative protective effects of taurine on protamine sulfate (PS) induced bladder injury. Wistar albino female rats were catheterized and intravesically infused with phosphate buffered solution (control group) or PS (PS group) dissolved in phosphate buffered solution. In the PS + taurine (PS+Tau) group, after the PS instillation, taurine (50 mg/kg) was injected intraperitoneally for 3 days. Histopathological changes were investigated by light and scanning electron microscopy. Tissue samples were also obtained to determine bladder malondialdehyde (MDA) (a biomarker of oxidative damage) and glutathione (GSH) (a biomarker of protective oxidative injury) levels. In the PS group ulcerated areas, an irregular mucus layer, inflammatory cell infiltration, and increased number of mast cells were observed. In the PS+Tau group, a relatively normal urothelial topography, glycosaminoglycan layer, and decreased number of mucosal mast cells and inflammatory cells were observed. Increased MDA levels as a result of PS induction lead us to propose that free radicals may have a critical role in this injury. The significant decrease in MDA and increase in GSH levels in the PS+Tau group compared to PS group was in accordance with morphological findings. Based on the results, taurine treatment significantly prevented PS induced degenerative morphological and biochemical changes of urinary bladder mucosa.
Pharmacology, 2005
Several studies demonstrate that taurine treatment prevents tissue damage in various models of in... more Several studies demonstrate that taurine treatment prevents tissue damage in various models of inflammation. Experiments have shown that chronic nicotine administration caused oxidant damage in various organs by increasing lipid peroxidation products and decreasing the activity of endogenous antioxidants. The aim of this study was to investigate the effects of taurine treatment on nicotine-induced oxidative changes in rat urinary bladder and kidney and to explore the possible mechanisms of action. Male Wistar albino rats were injected with nicotine hydrogen bitartrate (0.6 mg/kg i.p.) or saline for 21 days. Taurine was administered (50 mg/kg i.p.) alone or along with nicotine injections. At the end of the treatment period bladder tissue was used for in vitro contractility studies, or stored along with kidney tissue for the measurement of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen content. Tissue samples were also examined histolog...
Journal of Urology, 2005
The role of melatonin on chronic water avoidance stress (WAS) induced degeneration of bladder epi... more The role of melatonin on chronic water avoidance stress (WAS) induced degeneration of bladder epithelium (urothelium) was investigated. Wistar albino rats were exposed to WAS for 2 hours daily for 5 days (WAS group). Before exposing them to WAS 10 mg/kg melatonin (WAS plus melatonin group) was injected intraperitoneally into the animals. Bladder samples were investigated with light and electron microscopy. Lipid peroxidation and glutathione levels were also measured in the bladder. In the WAS only group an increased number of mast cells in the mucosa, ulcerated areas, vacuole formation and dilated perinuclear cisternae, and dilatation in the intercellular spaces in the urothelium were observed. In the WAS plus melatonin group relatively normal urothelial topography, a decreased number of mast cells in the mucosa, some dilatation between intercellular areas, regular perinuclear cisternae and tight junctions were observed. Increased lipid peroxidation and decreased glutathione levels in WAS rats were reversed by melatonin. The results show that melatonin reverses WAS induced degenerative changes in the bladder.
Journal of Medicinal Food, 2005
Fundamental and Clinical Pharmacology, 2005
Nicotine, a major toxic component of cigarette smoking, has long been recognized to result in oxi... more Nicotine, a major toxic component of cigarette smoking, has long been recognized to result in oxidative stress by inducing the generation of reactive oxygen species in the periphery, and central nervous system. Experiments have shown that chronic administration of nicotine caused increased lipid peroxidation products in serum and tissues of rats [1,2]. The increased concentration of lipid peroxidation products observed in nicotine treated rats was found to be associated with decreased activity of scavenging enzymes such as catalase and superoxide dismutase [2]. A decrease in the activities of these enzymes can lead to the excessive availability of superoxides and peroxyl radicals, which in turn generate hydroxyl radicals, resulting in the initiation and propagation of lipid peroxidation. Lipid peroxidation is known to cause cellular injury by inactivation of membrane enzymes and receptors, depolymerization of polysaccharide, as well as protein cross-linking and fragmentation. Since membrane lipids are vital for the maintenance and integrity of cell function, the breakdown of membrane phospholipids and lipid peroxidation are expected to change membrane structure, fluidity, transport and
Digestive Diseases and Sciences, 2006
We investigated the role of taurine, is a potent free radical scavenger, on water avoidance stres... more We investigated the role of taurine, is a potent free radical scavenger, on water avoidance stress (WAS)-induced degeneration of the gastric, ileal, and colonic mucosa and liver parenchyma. Wistar albino rats were exposed to chronic WAS (WAS group) 2 hr daily for 5 days. After exposing animals to chronic WAS (WAS + taurine group), 50 mg/kg taurine was injected IP for 3 days. Control animals received vehicle solution only. The stomach, ileum, colon, and liver samples were investigated under light microscope for histopathologic changes. To demonstrate the topography of the luminal mucosa of the stomach, ileum, and colon, scanning electron microscope was used and for hepatocyte ultastructure transmission electron microscope was used. Malondialdehyde (MDA, a biomarker of oxidative damage) and glutathione (GSH, a biomarker of protective oxidative injury) levels were also determined in all tissues. In the WAS group, the stomach epithelium showed ulceration in some areas, dilatations of the gastric glands, and degeneration of gastric glandular cells; prominent congestion of the capillaries was apparent. In the WAS group, severe vascular congestion was observed along with degeneration of ileal and colonic epithelium. Prominent vascular congestion and dilated sinusoids, activated Kupffer cells, dilated granular endoplasmic reticulum membranes, and focal pyknotic nuclei were observed in liver parenchyma. MDA levels (stomach, P < 0.01; ileum, colon, and liver P < 0.05) were increased and GSH levels (P < 0.01) were decreased in all tissues in the WAS group compared with the control group. The morphology of gastric, ileal, and colonic mucosa and liver parenchyma in the WAS + taurine group (stomach and ileum, P < 0.05; colon and liver, P < 0.01) showed a significant amelioration when compared to the WAS group. Increased MDA and decreased GSH levels in the WAS group were ameliorated with taurine treatment. Based on the results, taurine supplementation effectively attenuates the oxidative damage of gastrointestinal mucosa and liver because of WAS induction possibly by its antioxidant effects.
Digestive Diseases and Sciences, 2007
We investigated the effect of aqueous garlic extract (AGE) on water avoidance stress (WAS)-induce... more We investigated the effect of aqueous garlic extract (AGE) on water avoidance stress (WAS)-induced degeneration of the gastric and ileal mucosa and liver parenchyma. Wistar albino rats were exposed to WAS (WAS group) for 5 days. After exposure of the animals to WAS, a 1 ml/kg aqueous garlic extract (AGE) was injected i.p. (WAS+AGE group). The stomach, ileum, and liver samples were investigated under light microscope for general morphology. Topography of gastric and ileal mucosa was investigated by scanning electron microscopy, and hepatocyte ultastructure by transmission electron micsroscopy. Malondialdehyde (MDA) and glutathione (GSH) levels of all tissues were also determined. In the WAS group, the epithelium of the stomach showed ulceration in some areas, dilatations of the gastric glands, and degeneration of gastric glandular cells. Severe vascular congestion and degeneration of ileal epithelium were observed. Prominent vascular congestion and dilated sinusoids, activated Kupffer cells with prominent morphology, dilated granular endoplasmic reticulum membranes, and focal picnotic nuclei were observed in liver parenchyma. AGE treatment reduced the degeneration of the gastric and ileal mucosa and liver parenchyma. Increased MDA levels and decreased GSH levels in the WAS group were reversed to control values after AGE treatment. Based on these results, AGE treatment significantly prevented WAS-induced degeneration in both morphology and biochemistry of gastrointestinal mucosa and liver parenchyma due to its potent free radical scavenging and antioxidant properties.
Digestive Diseases and Sciences, 2005
mmj.dergisi.org
... 2-MERCAPTOETAN SÜLFONAT (MESNA)'IN, PROTAMİN SÜLFATIN TETİKLEDİĞİ MESANE HAS... more ... 2-MERCAPTOETAN SÜLFONAT (MESNA)'IN, PROTAMİN SÜLFATIN TETİKLEDİĞİ MESANE HASARI ÜZERİNDEKİ KORUYUCU ETKİLERİ. Beyhan Sağlam 1 , Esra Cikler 1 , Ali Zeybek 2 , Şule Çetinel 1 , Feriha Ercan 1 , Göksel Şener 3. ...
BJU International, 2006
To investigate the role of aqueous garlic extract (AGE) on the water-avoidance stress (WAS)-induc... more To investigate the role of aqueous garlic extract (AGE) on the water-avoidance stress (WAS)-induced degeneration of the urinary bladder in a rat model. Wistar albino rats were exposed to WAS for 2 h/day for 5 days (WAS group), after which, AGE (1 mL/kg) was injected intraperitoneally into the rats (WAS + AGE group). Urinary bladder samples were investigated with both light and scanning electron microscopy, and lipid peroxidation and glutathione levels were also measured in the samples. In the WAS group there was inflammatory cell infiltration, more mast cells and ulcerated areas in the mucosa. In the WAS + AGE group there was relatively normal urothelial alignment, moderate inflammatory cell infiltration and fewer mast cells in the mucosa. The increased lipid peroxidation and decreased glutathione levels in WAS rats were reversed by AGE treatment. These results show that AGE has a protective effect on WAS-induced degenerative changes in the urinary bladder.
mmj.dergisi.org
... 4) Huston DP, Bressler RB. Urticaria and angioedema. Med Clin North Am 1992; 76:805840. 5) O... more ... 4) Huston DP, Bressler RB. Urticaria and angioedema. Med Clin North Am 1992; 76:805840. 5) Ozdamar SO, Seckin D, Kandemir B, Turanli AY. Mast cells in psoriasis. Dermatology 1996; 192:189190. 6) Farber EM, Nickoloff BJ, Recht B, Fraki JE. ...