Esra Saglam - Academia.edu (original) (raw)

Papers by Esra Saglam

Biologia Futura, 2019

Introduction Hepatocellular carcinoma (HCC) is found within the first five most common tumors wor... more Introduction Hepatocellular carcinoma (HCC) is found within the first five most common tumors worldwide. Sorafenib is an approved agent in HCC treatment. Sertraline is a selective serotonin reuptake inhibitor. The aim of the study is to investigate the combination of sertraline and sorafenib at hepatocellular cancer cell proliferation and death. Methods HepG2 cells were treated with drugs and viability test XTT was performed. Cells were stained with hematoxylin and eosin for histological examination or with anti-Bcl-2 antibody and Hoechst 33258 for immunofluorescence. Results Viability results supported dose-dependent antiproliferative effect for both sertraline and sorafenib. Microscopic evaluation of stained cells exerts morphological changes. Discussion This is the first study to show that sorafenib and sertraline have synergistic effect in hepatocellular cancer.

Turkiye Klinikleri Journal of Case Reports, 2014

Turkiye Klinikleri Journal of Case Reports, 2015

Journal of Hazardous Materials, 2012

In this study pillar[5]arene (P5) and a quinoline-functionalized pillar[5]arene (P5-6Q) which is ... more In this study pillar[5]arene (P5) and a quinoline-functionalized pillar[5]arene (P5-6Q) which is used for detecting radioactive element, gas adsorption and toxic ions were synthesized. These materials were characterized by Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared (FTIR), elemental analysis, melting point, Mass Spectroscopy, Scanning Electron Microscopy (SEM) and Zeta Potential. The cytotoxic and genotoxic potential of P5 and P5-6Q at distinct concentrations of 12.5, 25, 50, and 100 μg/mL were also investigated by Allium ana-telophase and comet assays on Allium cepa roots and Drosophila melanogaster haemocytes. P5 and P5-6Q showed dose dependent cytotoxic effect by decreasing mitotic index (MI) and genotoxic effect by increasing chromosomal aberrations (CAs such as disturbed anaphase-telophase, polyploidy, stickiness, chromosome laggards and bridges) and DNA damage at the exposed concentrations. These changes in P5-6Q were lower than P5. Further research is necessary to clarify the cytotoxic and genotoxic action mechanisms of P5 and P5-6Q at molecular levels.

IEEE Transactions on Plasma Science, 2006

Background: Interest in the use of extremely low-frequency (ELF) electromagnetic field (EMF) for ... more Background: Interest in the use of extremely low-frequency (ELF) electromagnetic field (EMF) for the treatment of pain and inflammation is increasing due to the ability of this promising therapy to compete with pharmaceuticals without the adverse effects caused by drugs. However, there continues to be concerns regarding cytotoxic and genotoxic effects that may occur as a result of exposure to EMF. Objective: To investigate this concern, we tested the effect of our known therapeutic 5 Hz, 0.4 milliTesla (mT) EMF on a human mesenchymal stromal cell (hMSC) line to determine whether ELF-EMF exposure would cause cytotoxic or genotoxic effects. Methods: Treated samples along with controls were exposed to 5 Hz, 0.4 mT ELF-EMF for 20 min/day, 3Â/week for 2 weeks and then assayed for cell viability, proliferation rates, and chromosome breaks. Results: Cytogenetic analysis of the viability and proliferation rates along with analysis of morphological genome stability showed no cytotoxicity, and no chromosome breaks per karyotype analysis-therefore no genotoxicity. Conclusion: Exposure to an ELF-EMF of 5 Hz, 0.4 mT for 20 min/day, 3Â/week for 2 weeks does not cause cytotoxic or genotoxic effects in hMSCs.

European Neuropsychopharmacology, 2003

European Neuropsychopharmacology, 2003

The present study was designed to investigate the effects of fluoxetine, a selective serotonin re... more The present study was designed to investigate the effects of fluoxetine, a selective serotonin reuptake inhibitor, on ethanol withdrawal syndrome in rats. Adult male Wistar rats (218-255 g) were subjects. Ethanol (7.2%, v/v) was given to rats by a liquid diet for 21 days. Control rats were pair fed an isocaloric liquid diet containing sucrose as a caloric substitute to ethanol. Fluoxetine (2.5, 5 and 10 mg/kg) and saline were injected to rats intraperitoneally just before ethanol withdrawal. After 2nd, 4th and 6th hour of ethanol withdrawal, rats were observed for 5 min, and withdrawal signs that included locomotor hyperactivity, agitation, stereotyped behavior, wet dog shakes and tremor were recorded or rated. A second series of injections was given at 6 h after the first one, and subjects were then tested for audiogenic seizures. Fluoxetine produced some dose-dependent and significant inhibitory effects on all the signs of ethanol withdrawal during ethanol withdrawal period. Our results suggest that acute fluoxetine treatment has some beneficial effects on ethanol withdrawal in rats. Thus, this drug may be useful for treatment of ethanol withdrawal syndrome.

In view of the difficulties (1) in using antidepressant agents as training drugs in drug discrimi... more In view of the difficulties (1) in using antidepressant agents as training drugs in drug discrimination research, it was reasoned that tianeptine, which is a unique type of antidepressant (2) producing its effect by enhancing serotonin reuptake (3), because of its short duration of action and the lack of toxicity associated with its long-term administration, would be well-suited to establish a discriminative stimulus cue in rats and, hence a valuable tool in the investigation of neural basis of depression. Additionally, the discriminative stimulus properties of tianeptine has never been investigated before. A drug discrimination procedure was used to determine whether the tianeptine was associated with a specific discriminative stimulus effect; and, substitution tests were conducted to determine whether this effect was mediated by serotonergic mechanisms.

Folates are the vitamin B derivatives that play important roles on cell metabolism. They function... more Folates are the vitamin B derivatives that play important roles on cell metabolism. They function in cell proliferation, cell growth, amino acid and purine biosynthesis. Methylenetetrahydrofolate reductase enzyme is crucial for folate metabolism and some variations in the gene that encode the enzyme may cause some problems affecting enzyme kinetics. Absence of folates causes a range of pathologies from neural tube defects to congenital anomalies. Recently, variations in the methylenetetrahydrofolate reductase gene are associated with neurological and psychiatric diseases like Parkinson‟s, Alzheimer‟s and bipolar diseases and the role of these variations in these diseases were better diagnosed and described. In this review, upon giving brief information about folates, we aim to specify the association of methylenetetrahydrofolate reductase gene with Parkinson‟s, Alzheimer‟s, bipolar and schizophrenic diseases. (Anatolian Journal of Psychiatry 2013; 14:378-382)

Annals of Medical Research, 2020

Medical Journal of Bakirkoy, 2020

Objective: Medication errors are important concerns in terms of patient safety. Dose calculation ... more Objective: Medication errors are important concerns in terms of patient safety. Dose calculation skills contribute to medication errors. The aim of this study is to evaluate the calculation skills and self-ratings of nursing students. Method: Four multiple-choice questions with five alternative responses were asked and an electronic questionnaire form was used for the assessment of their perceptions of their self-competencies. Results: The rates of correct answers to the questions varied between 20% and 63%. In their self-assessments, 26.4% of them stated that they had sufficient dose calculation skills. Conclusion: The results of the study showed that skills should be improved. Drug dose calculation skills should be improved, and further education should be provided on this issue.

Turkiye Klinikleri Journal of Internal Medical Sciences, 2007

Toxicology and industrial health, Jan 23, 2015

In this study, the mutagenicity and genotoxicity of indium tin oxide (ITO) nanomaterial were asse... more In this study, the mutagenicity and genotoxicity of indium tin oxide (ITO) nanomaterial were assessed using two standard genotoxicity assays, the Salmonella reverse mutation assay (Ames test) and the in vitro micronucleus (MN) assay. Seven different concentrations (12.5, 25, 50, 75, 100, 125, and 150 µg/plate) of this nanomaterial were tested using the Ames test on the TA98 and TA100 strains in the presence and absence of the S9 mixture. At all the concentrations tested, this substance did not significantly increase the number of revertant colonies compared with the control with or without S9 mixture. The genotoxic effects of ITO were investigated in human peripheral lymphocytes treated with 125, 250, 500, and 750 µg/ml concentrations of this substance for 24- and 48-h treatment periods using an MN test. Nuclear division index (NDI) was also calculated in order to determine the cytotoxicity of ITO. It was determined that ITO increased MN frequency in the 750 µg/ml concentration in 2...

Transcranial direct current stimulation (TDCS) is a promising neuromodulatory treatment option in... more Transcranial direct current stimulation (TDCS) is a promising neuromodulatory treatment option in neuropsychiatry. However, such a novel neuromodulation application also brings challenges regarding safety. In this paper, we aimed to provide a retrospective evaluation of adverse effects related to TDCS implementation in a group of psychiatric patients. Twenty-nine patients who were on TDCS with different psychiatric diagnosis were evaluated retrospectively. Occurrence and severity of adverse events monitored with a questionnaire. No subjects experienced seizures or other adverse effects requiring medical intervention. During TDCS an itching sensation was the most common reported adverse effect (48 %, n=12) whereas after TDCS fatigue occurred in 32% of cases (n=8). Also one patient reported mild nausea during the first 3 sessions and one patient reported numbness in the electrode area. Transient redness at the site of stimulation was reported by 3 (12%) of patients. None of the report...

Cytotechnology, 2014

Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and gen... more Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and genotoxic effects of this pesticide on human peripheral blood lymphocytes and Allium root meristematic cells were investigated by chromosomal aberrations (CAs) and Allium test. Human lymphocytes were treated with 62.5, 125, 250 and 500 ppm doses of dioxacarb for CAs. CA/cell, abnormal cell % and mitotic index % (MI %) data were obtained from these concentrations in 24 and 48 h treatment periods. Dioxacarb did not increase the CA/cell frequency significantly, so this insecticide was not identified as genotoxic. But it was found cytotoxic especially at 250 and 500 ppm concentrations because of the reduced the MI % and increased the abnormal cell %. In Allium test, 25 ppm (EC50/2), 50 ppm (EC50) and 100 ppm (EC50 9 2) concentrations were used for root growth inhibition (EC50 determination) and Allium mitotic index (MI) determination tests. The used concentrations of dioxacarb induced dose-dependent inhibition of MI and root growth on root meristems. Mitotic inhibition of dioxacarb was found significantly higher than for the positive control. These Allium results indicated the high cytotoxicity of dioxacarb. The present study is the first research on cytotoxicity and genotoxicity of dioxacarb by human lymphocyte CAs and Allium test.

Therapeutic Drug Monitoring, 2015

There are limited studies investigating the relationship between oral release osmotic system-meth... more There are limited studies investigating the relationship between oral release osmotic system-methylphenidate (OROS-MPH) doses and plasma methylphenidate (MPH) concentrations in children and adolescents. The aim of the present study was to investigate the relationship between the doses of OROS-MPH and the plasma levels of the drug. We also examined the effects of the other drugs including aripiprazole, risperidone, fluoxetine, and sertraline on the levels of the MPH in the plasma. The files of 100 ADHD subjects (76 male, 24 female) who were diagnosed as ADHD according to the DSM-IV criteria, were screened. The ages of subjects were between 6-18 years (mean= 11.5±3.8 years). Plasma MPH levels were determined by high-performance liquid chromatography-tandem mass spectrometry assay (HPLC-MS/MS). Daily mean OROS MPH dose used in ADHD children was 0.7±0.2 mg/kg (range: 0.3-1.3 mg/kg). The mean plasma OROS MPH was 11.6 ± 7.3 ng/mL (range: 0.5-43.4 ng/mL). There was no group difference in the mean plasma MPH and dose-related MPH levels between the groups that used any additional drug including aripiprazole (n=25), risperidone (n=10), fluoxetine (n=16) sertraline (n=10) and not used these drugs (p>.05). There was a positive correlation between the OROS MPH doses (mg/kg) and the blood MPH levels (Pearson correlation= 0.40; p<.001). The plasma levels of MPH were found to be less than 13 ng/mL in 65% of the subjects. Our findings point to the fact that plasma levels of MPH show a wide range of changes at similar doses, correlate positively with the doses and, as expected, are not affected by using risperidone, sertraline, fluoxetine, and aripiprazole. Therapeutic drug monitoring (TDM) may help to optimize MPH dose in patients not responding to treatment or in those experiencing serious side effects, but not in routine clinical practice. The presence of intermediate dose formulations such as 45 mg tablets for OROS MPH may contribute to the optimization.

Psychogeriatrics, 2014

Several studies have reported that depression and anxiety are very common in atrial fibrillation ... more Several studies have reported that depression and anxiety are very common in atrial fibrillation due to impaired quality of life. Dabigatran is an anti-aggregation agent used for the treatment of atrial fibrillation. In terms of drug interactions during treatment with dabigatran, patients suffering from minor depression are reported to be a population at risk. This report is about a 68-year-old man whose depressive symptoms were aggravated after taking dabigatran for atrial fibrillation. The case is discussed in terms of his aggravated depressive symptoms and the interaction between his prescription medications.

NPAKADEMI, 2012

Duloksetin çifte etki mekanizmasına sahip (seçici serotonin ve norepinefrin geri alım inhibitörü)... more Duloksetin çifte etki mekanizmasına sahip (seçici serotonin ve norepinefrin geri alım inhibitörü) bir antidepresandır. Araştırmalar, duloksetin için önerilen kan düzeyini 20-80 ng/mL olarak vermektedir. Bu çalışmada 30-90 mg/gün dozlarında kullanılmış olan oral duloksetinin farmakokinetiğinin incelenmesi amaçlanmıştır. Yöntem: Ayaktan izlenen depresyon hastalarında kararlı düzeye ulaşmış olan duloksetin kan düzeyleri sıvı kromatografi-kütle spektroskopi tekniği ile ölçülmüştür. Hastalar 30-90 mg/gün oral duloksetini günde tek doz olarak kullanmaktaydı. Hastaların klinik iyileşmesi ve ilacı tolere etmelerine göre, duloksetin dozları esnek biçimde klinisyence ayarlanmıştı. Çalışma grubunda 60 kadın ve 53 erkek bulunmaktaydı. Duloksetinin kan düzeyleri tedavinin 7.-180. Günleri arasında bir dönemde, son ilaç dozundan 24 saat sonra ölçüldü. Bulgular: Sigara içenlerde günlük duloksetin dozu daha yüksekken, duloksetinin kan düzeylerinde sigara içmeyenlere göre farklılık saptanmadı. Vücut ağırlığı ve yaşın duloksetinin farmakokinetik parametrelerine etkisi gözlenmedi. Sonuç: Araştırma sonuçlarına göre duloksetin kan düzeyi ile klinik yanıtı ilişkili değildir. Ancak sigara içenler içmeyenlere göre daha yüksek dozlara gereksinme duymaktadır.

Turkish Journal of Trauma and Emergency Surgery, 2014

Biologia Futura, 2019

Introduction Hepatocellular carcinoma (HCC) is found within the first five most common tumors wor... more Introduction Hepatocellular carcinoma (HCC) is found within the first five most common tumors worldwide. Sorafenib is an approved agent in HCC treatment. Sertraline is a selective serotonin reuptake inhibitor. The aim of the study is to investigate the combination of sertraline and sorafenib at hepatocellular cancer cell proliferation and death. Methods HepG2 cells were treated with drugs and viability test XTT was performed. Cells were stained with hematoxylin and eosin for histological examination or with anti-Bcl-2 antibody and Hoechst 33258 for immunofluorescence. Results Viability results supported dose-dependent antiproliferative effect for both sertraline and sorafenib. Microscopic evaluation of stained cells exerts morphological changes. Discussion This is the first study to show that sorafenib and sertraline have synergistic effect in hepatocellular cancer.

Turkiye Klinikleri Journal of Case Reports, 2014

Turkiye Klinikleri Journal of Case Reports, 2015

Journal of Hazardous Materials, 2012

In this study pillar[5]arene (P5) and a quinoline-functionalized pillar[5]arene (P5-6Q) which is ... more In this study pillar[5]arene (P5) and a quinoline-functionalized pillar[5]arene (P5-6Q) which is used for detecting radioactive element, gas adsorption and toxic ions were synthesized. These materials were characterized by Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared (FTIR), elemental analysis, melting point, Mass Spectroscopy, Scanning Electron Microscopy (SEM) and Zeta Potential. The cytotoxic and genotoxic potential of P5 and P5-6Q at distinct concentrations of 12.5, 25, 50, and 100 μg/mL were also investigated by Allium ana-telophase and comet assays on Allium cepa roots and Drosophila melanogaster haemocytes. P5 and P5-6Q showed dose dependent cytotoxic effect by decreasing mitotic index (MI) and genotoxic effect by increasing chromosomal aberrations (CAs such as disturbed anaphase-telophase, polyploidy, stickiness, chromosome laggards and bridges) and DNA damage at the exposed concentrations. These changes in P5-6Q were lower than P5. Further research is necessary to clarify the cytotoxic and genotoxic action mechanisms of P5 and P5-6Q at molecular levels.

IEEE Transactions on Plasma Science, 2006

Background: Interest in the use of extremely low-frequency (ELF) electromagnetic field (EMF) for ... more Background: Interest in the use of extremely low-frequency (ELF) electromagnetic field (EMF) for the treatment of pain and inflammation is increasing due to the ability of this promising therapy to compete with pharmaceuticals without the adverse effects caused by drugs. However, there continues to be concerns regarding cytotoxic and genotoxic effects that may occur as a result of exposure to EMF. Objective: To investigate this concern, we tested the effect of our known therapeutic 5 Hz, 0.4 milliTesla (mT) EMF on a human mesenchymal stromal cell (hMSC) line to determine whether ELF-EMF exposure would cause cytotoxic or genotoxic effects. Methods: Treated samples along with controls were exposed to 5 Hz, 0.4 mT ELF-EMF for 20 min/day, 3Â/week for 2 weeks and then assayed for cell viability, proliferation rates, and chromosome breaks. Results: Cytogenetic analysis of the viability and proliferation rates along with analysis of morphological genome stability showed no cytotoxicity, and no chromosome breaks per karyotype analysis-therefore no genotoxicity. Conclusion: Exposure to an ELF-EMF of 5 Hz, 0.4 mT for 20 min/day, 3Â/week for 2 weeks does not cause cytotoxic or genotoxic effects in hMSCs.

European Neuropsychopharmacology, 2003

European Neuropsychopharmacology, 2003

The present study was designed to investigate the effects of fluoxetine, a selective serotonin re... more The present study was designed to investigate the effects of fluoxetine, a selective serotonin reuptake inhibitor, on ethanol withdrawal syndrome in rats. Adult male Wistar rats (218-255 g) were subjects. Ethanol (7.2%, v/v) was given to rats by a liquid diet for 21 days. Control rats were pair fed an isocaloric liquid diet containing sucrose as a caloric substitute to ethanol. Fluoxetine (2.5, 5 and 10 mg/kg) and saline were injected to rats intraperitoneally just before ethanol withdrawal. After 2nd, 4th and 6th hour of ethanol withdrawal, rats were observed for 5 min, and withdrawal signs that included locomotor hyperactivity, agitation, stereotyped behavior, wet dog shakes and tremor were recorded or rated. A second series of injections was given at 6 h after the first one, and subjects were then tested for audiogenic seizures. Fluoxetine produced some dose-dependent and significant inhibitory effects on all the signs of ethanol withdrawal during ethanol withdrawal period. Our results suggest that acute fluoxetine treatment has some beneficial effects on ethanol withdrawal in rats. Thus, this drug may be useful for treatment of ethanol withdrawal syndrome.

In view of the difficulties (1) in using antidepressant agents as training drugs in drug discrimi... more In view of the difficulties (1) in using antidepressant agents as training drugs in drug discrimination research, it was reasoned that tianeptine, which is a unique type of antidepressant (2) producing its effect by enhancing serotonin reuptake (3), because of its short duration of action and the lack of toxicity associated with its long-term administration, would be well-suited to establish a discriminative stimulus cue in rats and, hence a valuable tool in the investigation of neural basis of depression. Additionally, the discriminative stimulus properties of tianeptine has never been investigated before. A drug discrimination procedure was used to determine whether the tianeptine was associated with a specific discriminative stimulus effect; and, substitution tests were conducted to determine whether this effect was mediated by serotonergic mechanisms.

Folates are the vitamin B derivatives that play important roles on cell metabolism. They function... more Folates are the vitamin B derivatives that play important roles on cell metabolism. They function in cell proliferation, cell growth, amino acid and purine biosynthesis. Methylenetetrahydrofolate reductase enzyme is crucial for folate metabolism and some variations in the gene that encode the enzyme may cause some problems affecting enzyme kinetics. Absence of folates causes a range of pathologies from neural tube defects to congenital anomalies. Recently, variations in the methylenetetrahydrofolate reductase gene are associated with neurological and psychiatric diseases like Parkinson‟s, Alzheimer‟s and bipolar diseases and the role of these variations in these diseases were better diagnosed and described. In this review, upon giving brief information about folates, we aim to specify the association of methylenetetrahydrofolate reductase gene with Parkinson‟s, Alzheimer‟s, bipolar and schizophrenic diseases. (Anatolian Journal of Psychiatry 2013; 14:378-382)

Annals of Medical Research, 2020

Medical Journal of Bakirkoy, 2020

Objective: Medication errors are important concerns in terms of patient safety. Dose calculation ... more Objective: Medication errors are important concerns in terms of patient safety. Dose calculation skills contribute to medication errors. The aim of this study is to evaluate the calculation skills and self-ratings of nursing students. Method: Four multiple-choice questions with five alternative responses were asked and an electronic questionnaire form was used for the assessment of their perceptions of their self-competencies. Results: The rates of correct answers to the questions varied between 20% and 63%. In their self-assessments, 26.4% of them stated that they had sufficient dose calculation skills. Conclusion: The results of the study showed that skills should be improved. Drug dose calculation skills should be improved, and further education should be provided on this issue.

Turkiye Klinikleri Journal of Internal Medical Sciences, 2007

Toxicology and industrial health, Jan 23, 2015

In this study, the mutagenicity and genotoxicity of indium tin oxide (ITO) nanomaterial were asse... more In this study, the mutagenicity and genotoxicity of indium tin oxide (ITO) nanomaterial were assessed using two standard genotoxicity assays, the Salmonella reverse mutation assay (Ames test) and the in vitro micronucleus (MN) assay. Seven different concentrations (12.5, 25, 50, 75, 100, 125, and 150 µg/plate) of this nanomaterial were tested using the Ames test on the TA98 and TA100 strains in the presence and absence of the S9 mixture. At all the concentrations tested, this substance did not significantly increase the number of revertant colonies compared with the control with or without S9 mixture. The genotoxic effects of ITO were investigated in human peripheral lymphocytes treated with 125, 250, 500, and 750 µg/ml concentrations of this substance for 24- and 48-h treatment periods using an MN test. Nuclear division index (NDI) was also calculated in order to determine the cytotoxicity of ITO. It was determined that ITO increased MN frequency in the 750 µg/ml concentration in 2...

Transcranial direct current stimulation (TDCS) is a promising neuromodulatory treatment option in... more Transcranial direct current stimulation (TDCS) is a promising neuromodulatory treatment option in neuropsychiatry. However, such a novel neuromodulation application also brings challenges regarding safety. In this paper, we aimed to provide a retrospective evaluation of adverse effects related to TDCS implementation in a group of psychiatric patients. Twenty-nine patients who were on TDCS with different psychiatric diagnosis were evaluated retrospectively. Occurrence and severity of adverse events monitored with a questionnaire. No subjects experienced seizures or other adverse effects requiring medical intervention. During TDCS an itching sensation was the most common reported adverse effect (48 %, n=12) whereas after TDCS fatigue occurred in 32% of cases (n=8). Also one patient reported mild nausea during the first 3 sessions and one patient reported numbness in the electrode area. Transient redness at the site of stimulation was reported by 3 (12%) of patients. None of the report...

Cytotechnology, 2014

Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and gen... more Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and genotoxic effects of this pesticide on human peripheral blood lymphocytes and Allium root meristematic cells were investigated by chromosomal aberrations (CAs) and Allium test. Human lymphocytes were treated with 62.5, 125, 250 and 500 ppm doses of dioxacarb for CAs. CA/cell, abnormal cell % and mitotic index % (MI %) data were obtained from these concentrations in 24 and 48 h treatment periods. Dioxacarb did not increase the CA/cell frequency significantly, so this insecticide was not identified as genotoxic. But it was found cytotoxic especially at 250 and 500 ppm concentrations because of the reduced the MI % and increased the abnormal cell %. In Allium test, 25 ppm (EC50/2), 50 ppm (EC50) and 100 ppm (EC50 9 2) concentrations were used for root growth inhibition (EC50 determination) and Allium mitotic index (MI) determination tests. The used concentrations of dioxacarb induced dose-dependent inhibition of MI and root growth on root meristems. Mitotic inhibition of dioxacarb was found significantly higher than for the positive control. These Allium results indicated the high cytotoxicity of dioxacarb. The present study is the first research on cytotoxicity and genotoxicity of dioxacarb by human lymphocyte CAs and Allium test.

Therapeutic Drug Monitoring, 2015

There are limited studies investigating the relationship between oral release osmotic system-meth... more There are limited studies investigating the relationship between oral release osmotic system-methylphenidate (OROS-MPH) doses and plasma methylphenidate (MPH) concentrations in children and adolescents. The aim of the present study was to investigate the relationship between the doses of OROS-MPH and the plasma levels of the drug. We also examined the effects of the other drugs including aripiprazole, risperidone, fluoxetine, and sertraline on the levels of the MPH in the plasma. The files of 100 ADHD subjects (76 male, 24 female) who were diagnosed as ADHD according to the DSM-IV criteria, were screened. The ages of subjects were between 6-18 years (mean= 11.5±3.8 years). Plasma MPH levels were determined by high-performance liquid chromatography-tandem mass spectrometry assay (HPLC-MS/MS). Daily mean OROS MPH dose used in ADHD children was 0.7±0.2 mg/kg (range: 0.3-1.3 mg/kg). The mean plasma OROS MPH was 11.6 ± 7.3 ng/mL (range: 0.5-43.4 ng/mL). There was no group difference in the mean plasma MPH and dose-related MPH levels between the groups that used any additional drug including aripiprazole (n=25), risperidone (n=10), fluoxetine (n=16) sertraline (n=10) and not used these drugs (p>.05). There was a positive correlation between the OROS MPH doses (mg/kg) and the blood MPH levels (Pearson correlation= 0.40; p<.001). The plasma levels of MPH were found to be less than 13 ng/mL in 65% of the subjects. Our findings point to the fact that plasma levels of MPH show a wide range of changes at similar doses, correlate positively with the doses and, as expected, are not affected by using risperidone, sertraline, fluoxetine, and aripiprazole. Therapeutic drug monitoring (TDM) may help to optimize MPH dose in patients not responding to treatment or in those experiencing serious side effects, but not in routine clinical practice. The presence of intermediate dose formulations such as 45 mg tablets for OROS MPH may contribute to the optimization.

Psychogeriatrics, 2014

Several studies have reported that depression and anxiety are very common in atrial fibrillation ... more Several studies have reported that depression and anxiety are very common in atrial fibrillation due to impaired quality of life. Dabigatran is an anti-aggregation agent used for the treatment of atrial fibrillation. In terms of drug interactions during treatment with dabigatran, patients suffering from minor depression are reported to be a population at risk. This report is about a 68-year-old man whose depressive symptoms were aggravated after taking dabigatran for atrial fibrillation. The case is discussed in terms of his aggravated depressive symptoms and the interaction between his prescription medications.

NPAKADEMI, 2012

Duloksetin çifte etki mekanizmasına sahip (seçici serotonin ve norepinefrin geri alım inhibitörü)... more Duloksetin çifte etki mekanizmasına sahip (seçici serotonin ve norepinefrin geri alım inhibitörü) bir antidepresandır. Araştırmalar, duloksetin için önerilen kan düzeyini 20-80 ng/mL olarak vermektedir. Bu çalışmada 30-90 mg/gün dozlarında kullanılmış olan oral duloksetinin farmakokinetiğinin incelenmesi amaçlanmıştır. Yöntem: Ayaktan izlenen depresyon hastalarında kararlı düzeye ulaşmış olan duloksetin kan düzeyleri sıvı kromatografi-kütle spektroskopi tekniği ile ölçülmüştür. Hastalar 30-90 mg/gün oral duloksetini günde tek doz olarak kullanmaktaydı. Hastaların klinik iyileşmesi ve ilacı tolere etmelerine göre, duloksetin dozları esnek biçimde klinisyence ayarlanmıştı. Çalışma grubunda 60 kadın ve 53 erkek bulunmaktaydı. Duloksetinin kan düzeyleri tedavinin 7.-180. Günleri arasında bir dönemde, son ilaç dozundan 24 saat sonra ölçüldü. Bulgular: Sigara içenlerde günlük duloksetin dozu daha yüksekken, duloksetinin kan düzeylerinde sigara içmeyenlere göre farklılık saptanmadı. Vücut ağırlığı ve yaşın duloksetinin farmakokinetik parametrelerine etkisi gözlenmedi. Sonuç: Araştırma sonuçlarına göre duloksetin kan düzeyi ile klinik yanıtı ilişkili değildir. Ancak sigara içenler içmeyenlere göre daha yüksek dozlara gereksinme duymaktadır.

Turkish Journal of Trauma and Emergency Surgery, 2014