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Papers by Estefan Miranda

Research paper thumbnail of Poster Session 2

Journal of Veterinary Pharmacology and Therapeutics, 2012

Research paper thumbnail of Heterologus Gene Expression in a Cattle Tick Rhipicephalus microplus Embryonic Cell Culture

Journal of Animal and …, 2007

... in a Cattle Tick Rhipicephalus microplus Embryonic Cell Culture Raquel Cossio-Bayugar and Est... more ... in a Cattle Tick Rhipicephalus microplus Embryonic Cell Culture Raquel Cossio-Bayugar and Estefan Miranda-Miranda Centro Nacional ... of action which in case of pyrethroids and organophos-phates are the sodium channel and Acetylcolinesterase (AchE) respectively (French ...

Research paper thumbnail of Additional file 9: Table S7. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of detoxifying enzymes. (XLSX 3441Â kb)

Research paper thumbnail of Additional file 8: Table S6. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of redox genes. (XLSX 40Â kb)

Research paper thumbnail of Additional file 7: Table S5. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of collagen genes. (XLSX 51Â kb)

Research paper thumbnail of Additional file 6: Table S4. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of cytoskeleton related gene families. (XLSX 23Â kb)

Research paper thumbnail of Additional file 5: Table S3. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

GO category overrepresentation analysis for each two-way comparison. (XLSX 23Â kb)

Research paper thumbnail of Additional file 4: Table S2. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Top 20 upregulated (a) and downregulated (b) genes for each pairwise comparison. (XLSX 16Â kb)

Research paper thumbnail of Additional file 3: Figure S2. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

a Correlation of normalized counts and housekeeping genes. Scatterplot showing the correlation of... more a Correlation of normalized counts and housekeeping genes. Scatterplot showing the correlation of Cenapa normalized counts per gene versus their Cajamarca counterparts. b Scatterplot showing the correlation of Cenapa normalized counts per gene versus their Rubino counterparts. Full circles highlight the expression of genes belonging to housekeeping functions. Colors are as in Fig. 2. (TIFF 1042 kb)

Research paper thumbnail of Additional file 1: Table S1. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

General data processing overview. (XLSX 10Â kb)

Research paper thumbnail of Additional file 10: Table S8. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differentially expression of genes identified in proteomic study. (XLSX 19Â kb)

Research paper thumbnail of Adrenergic ligands that block oviposition in the cattle tick Rhipicephalus microplus affect ovary contraction

Scientific Reports, 2015

The tyraminergic/octopaminergic system is central for the control of arthropod oviposition. Previ... more The tyraminergic/octopaminergic system is central for the control of arthropod oviposition. Previous works demonstrated that the pharmacological perturbation of this system inhibits oviposition in the cattle tick Rhipicephalus microplus. In this work, we describe a physiologically active wholemount preparation of the contractile tick ovary that allows the quantitative videometrical analysis of ovary contraction in response to different compounds. Eight adrenergic ligands known to inhibit oviposition, including octopamine and tyramine were tested. These compounds exhibited antagonistic effects; octopamine relaxes the ovary preparation while tyramine induces a very strong contraction. The other adrenergic compounds tested were classified as able to contract or relax ovary muscle tissue. Isoprotenerol has a stronger relaxative effect than octopamine. Tyramine induces the biggest contraction observed of all the compounds tested, followed, in descending amount of contraction, by salbutamol, prazosin, epinastine, clonidine and the acaricide amitraz. The effect of these adrenergic ligands on the ovary preparation, explains why these molecules inhibit tick oviposition and suggest a regulatory mechanism for ovary contraction and relaxation during oviposition. Our results also provide a physiological explanation of the egg-laying inhibition effect of amitraz when used on the cattle tick.

Research paper thumbnail of Additional file 2: Figure S1. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression between isolate pairs. Volcano plot showing the differential expression o... more Differential expression between isolate pairs. Volcano plot showing the differential expression of transcripts between Cajamarca and Cenapa (a) and between Rubino and Cenapa (b). Red dots represent differentially expressed genes (log2 fold change > 2, P-value

Research paper thumbnail of Cloning of esterase genes from the cattle tick Boophilus microplus

Research paper thumbnail of Transcriptome assembly dataset of anthelmintic response in Fasciola hepatica

Fasciola hepatica is a worldwide distributed zoonotic parasitic trematode, which causes a severe ... more Fasciola hepatica is a worldwide distributed zoonotic parasitic trematode, which causes a severe liver disease clinically known as fasciolasis in a large number of wild animals, several livestock species as well as humans, prevention and control of fasciolasis is made by massive use of anthelmintic compounds on livestock and inevitably this practice has led to the emergence of anthelmintic resistant Fasciola hepatica and there is a great scientific effort to elucidate the molecular basis of anthelmintic resistance of parasitic helminths in general and of Fasciola hepatica in particular that may lead to improved anthelmintic compounds. In our project, we sequenced the transcriptomes obtained from the anthelmintic response to Triclabendazole and Albendazole on four samples from sensitive and resistant strains of Fasciola hepatica on Illumina HiSeq 4000 Platform and generated about 10.03 Gb per sample. The average genome-mapping rate is 81.29% and the average gene-mapping rate is 62.81...

Research paper thumbnail of Dr. estefan miranda-Miranda

Research paper thumbnail of REDVET. Revista Electrónica de Veterinaria

Resumen es: Objetivo: Determinar el efecto de la sedacion con dexmedetomidina sobre la presion in... more Resumen es: Objetivo: Determinar el efecto de la sedacion con dexmedetomidina sobre la presion intraocular (PIO) y el espesor central de la cornea (ECC) en perros Ma...

Research paper thumbnail of Identification and cloning of esterases from the tick Boophilus microplus resistant to organophosphate ixodicides

Publicacion …, 1995

... Source, [3. International seminary on Animal Parasitology. Acapulco, Guerrero (Mexico). 11-13... more ... Source, [3. International seminary on Animal Parasitology. Acapulco, Guerrero (Mexico). 11-13 Oct 1995 (Proccedings)], Rodriguez Camarillo, Sergio DarioFragoso Sanchez, Hugo (Ed.).- Mexico, DF: SAGAR, Oct 1995. ... Avenida Progreso No 5. Col. Barrio de Santa Catarina,. ...

Research paper thumbnail of Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B

Pathogens

Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of... more Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 amino acids, resulting in a predicted protein mass of 83.5 kDa and a putative carboxylesterase B enzyme. The gene coding for this enzyme was found in two reported F. hepatica complete genomes stretching 23,230 bp, containing two exons of 1282 and 1864 bp, respectively, as well as a 20,084 bp intron between the exons. The enzymatic activity was experimentally assayed on F. hepatica protein extracts by SDS-PAGE zymograms using synthetic chromogenic substrates, confirming both the theoretical molecular weight and carboxylesterase enzymatic activity. Further bioinformatics predicted that this enzyme is an integral component of the cellular membrane that should be active as a 167 kDa homodimer complex and polyacrylamide gel electrophoresis (...

Research paper thumbnail of Megaselia scalaris reared on Rhipicephalus (Boophilus) microplus laboratory cultures

Medical and Veterinary Entomology, 2011

Different laboratory cultures of the acarine tick Rhipicephalus (Boophilus) microplus (Canestrini... more Different laboratory cultures of the acarine tick Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Ixodida: Ixodidae) were infested by small Megaselia scalaris (Loew, 1866) (Diptera: Phoridae) flies. Larvae of this species exhibited opportunistic parasitism predominantly on engorged female ticks, causing severe damage to their cuticle through which the flies were able to reach R. microplus internal organs, on which they fed until developing into pupae in the tick's remains. The flies were kept by continuous propagation on fresh ticks over six generations during which the same parasitoid behaviour was observed. Here we report on an ixodid tick laboratory culture used for rearing M. scalaris.

Research paper thumbnail of Poster Session 2

Journal of Veterinary Pharmacology and Therapeutics, 2012

Research paper thumbnail of Heterologus Gene Expression in a Cattle Tick Rhipicephalus microplus Embryonic Cell Culture

Journal of Animal and …, 2007

... in a Cattle Tick Rhipicephalus microplus Embryonic Cell Culture Raquel Cossio-Bayugar and Est... more ... in a Cattle Tick Rhipicephalus microplus Embryonic Cell Culture Raquel Cossio-Bayugar and Estefan Miranda-Miranda Centro Nacional ... of action which in case of pyrethroids and organophos-phates are the sodium channel and Acetylcolinesterase (AchE) respectively (French ...

Research paper thumbnail of Additional file 9: Table S7. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of detoxifying enzymes. (XLSX 3441Â kb)

Research paper thumbnail of Additional file 8: Table S6. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of redox genes. (XLSX 40Â kb)

Research paper thumbnail of Additional file 7: Table S5. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of collagen genes. (XLSX 51Â kb)

Research paper thumbnail of Additional file 6: Table S4. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression of cytoskeleton related gene families. (XLSX 23Â kb)

Research paper thumbnail of Additional file 5: Table S3. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

GO category overrepresentation analysis for each two-way comparison. (XLSX 23Â kb)

Research paper thumbnail of Additional file 4: Table S2. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Top 20 upregulated (a) and downregulated (b) genes for each pairwise comparison. (XLSX 16Â kb)

Research paper thumbnail of Additional file 3: Figure S2. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

a Correlation of normalized counts and housekeeping genes. Scatterplot showing the correlation of... more a Correlation of normalized counts and housekeeping genes. Scatterplot showing the correlation of Cenapa normalized counts per gene versus their Cajamarca counterparts. b Scatterplot showing the correlation of Cenapa normalized counts per gene versus their Rubino counterparts. Full circles highlight the expression of genes belonging to housekeeping functions. Colors are as in Fig. 2. (TIFF 1042 kb)

Research paper thumbnail of Additional file 1: Table S1. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

General data processing overview. (XLSX 10Â kb)

Research paper thumbnail of Additional file 10: Table S8. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differentially expression of genes identified in proteomic study. (XLSX 19Â kb)

Research paper thumbnail of Adrenergic ligands that block oviposition in the cattle tick Rhipicephalus microplus affect ovary contraction

Scientific Reports, 2015

The tyraminergic/octopaminergic system is central for the control of arthropod oviposition. Previ... more The tyraminergic/octopaminergic system is central for the control of arthropod oviposition. Previous works demonstrated that the pharmacological perturbation of this system inhibits oviposition in the cattle tick Rhipicephalus microplus. In this work, we describe a physiologically active wholemount preparation of the contractile tick ovary that allows the quantitative videometrical analysis of ovary contraction in response to different compounds. Eight adrenergic ligands known to inhibit oviposition, including octopamine and tyramine were tested. These compounds exhibited antagonistic effects; octopamine relaxes the ovary preparation while tyramine induces a very strong contraction. The other adrenergic compounds tested were classified as able to contract or relax ovary muscle tissue. Isoprotenerol has a stronger relaxative effect than octopamine. Tyramine induces the biggest contraction observed of all the compounds tested, followed, in descending amount of contraction, by salbutamol, prazosin, epinastine, clonidine and the acaricide amitraz. The effect of these adrenergic ligands on the ovary preparation, explains why these molecules inhibit tick oviposition and suggest a regulatory mechanism for ovary contraction and relaxation during oviposition. Our results also provide a physiological explanation of the egg-laying inhibition effect of amitraz when used on the cattle tick.

Research paper thumbnail of Additional file 2: Figure S1. of Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Differential expression between isolate pairs. Volcano plot showing the differential expression o... more Differential expression between isolate pairs. Volcano plot showing the differential expression of transcripts between Cajamarca and Cenapa (a) and between Rubino and Cenapa (b). Red dots represent differentially expressed genes (log2 fold change > 2, P-value

Research paper thumbnail of Cloning of esterase genes from the cattle tick Boophilus microplus

Research paper thumbnail of Transcriptome assembly dataset of anthelmintic response in Fasciola hepatica

Fasciola hepatica is a worldwide distributed zoonotic parasitic trematode, which causes a severe ... more Fasciola hepatica is a worldwide distributed zoonotic parasitic trematode, which causes a severe liver disease clinically known as fasciolasis in a large number of wild animals, several livestock species as well as humans, prevention and control of fasciolasis is made by massive use of anthelmintic compounds on livestock and inevitably this practice has led to the emergence of anthelmintic resistant Fasciola hepatica and there is a great scientific effort to elucidate the molecular basis of anthelmintic resistance of parasitic helminths in general and of Fasciola hepatica in particular that may lead to improved anthelmintic compounds. In our project, we sequenced the transcriptomes obtained from the anthelmintic response to Triclabendazole and Albendazole on four samples from sensitive and resistant strains of Fasciola hepatica on Illumina HiSeq 4000 Platform and generated about 10.03 Gb per sample. The average genome-mapping rate is 81.29% and the average gene-mapping rate is 62.81...

Research paper thumbnail of Dr. estefan miranda-Miranda

Research paper thumbnail of REDVET. Revista Electrónica de Veterinaria

Resumen es: Objetivo: Determinar el efecto de la sedacion con dexmedetomidina sobre la presion in... more Resumen es: Objetivo: Determinar el efecto de la sedacion con dexmedetomidina sobre la presion intraocular (PIO) y el espesor central de la cornea (ECC) en perros Ma...

Research paper thumbnail of Identification and cloning of esterases from the tick Boophilus microplus resistant to organophosphate ixodicides

Publicacion …, 1995

... Source, [3. International seminary on Animal Parasitology. Acapulco, Guerrero (Mexico). 11-13... more ... Source, [3. International seminary on Animal Parasitology. Acapulco, Guerrero (Mexico). 11-13 Oct 1995 (Proccedings)], Rodriguez Camarillo, Sergio DarioFragoso Sanchez, Hugo (Ed.).- Mexico, DF: SAGAR, Oct 1995. ... Avenida Progreso No 5. Col. Barrio de Santa Catarina,. ...

Research paper thumbnail of Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B

Pathogens

Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of... more Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 amino acids, resulting in a predicted protein mass of 83.5 kDa and a putative carboxylesterase B enzyme. The gene coding for this enzyme was found in two reported F. hepatica complete genomes stretching 23,230 bp, containing two exons of 1282 and 1864 bp, respectively, as well as a 20,084 bp intron between the exons. The enzymatic activity was experimentally assayed on F. hepatica protein extracts by SDS-PAGE zymograms using synthetic chromogenic substrates, confirming both the theoretical molecular weight and carboxylesterase enzymatic activity. Further bioinformatics predicted that this enzyme is an integral component of the cellular membrane that should be active as a 167 kDa homodimer complex and polyacrylamide gel electrophoresis (...

Research paper thumbnail of Megaselia scalaris reared on Rhipicephalus (Boophilus) microplus laboratory cultures

Medical and Veterinary Entomology, 2011

Different laboratory cultures of the acarine tick Rhipicephalus (Boophilus) microplus (Canestrini... more Different laboratory cultures of the acarine tick Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Ixodida: Ixodidae) were infested by small Megaselia scalaris (Loew, 1866) (Diptera: Phoridae) flies. Larvae of this species exhibited opportunistic parasitism predominantly on engorged female ticks, causing severe damage to their cuticle through which the flies were able to reach R. microplus internal organs, on which they fed until developing into pupae in the tick's remains. The flies were kept by continuous propagation on fresh ticks over six generations during which the same parasitoid behaviour was observed. Here we report on an ixodid tick laboratory culture used for rearing M. scalaris.