Eulogia Hinako Kairiyama - Academia.edu (original) (raw)
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Papers by Eulogia Hinako Kairiyama
Radiation Physics and Chemistry, 2002
Samples of nine different papers in use in the library, including two mould-contaminated papers, ... more Samples of nine different papers in use in the library, including two mould-contaminated papers, were irradiated with 14.4 kGy in a Co-60 facility. Five of the samples were subsequently subjected to an accelerated UV-ageing process. mechanical (tensile energy ...
Radiation Physics and Chemistry, 1997
Dehydrated pancreatin, packed in polyethylene bags, was gamma irradiated with doses of 5, 10, 15 ... more Dehydrated pancreatin, packed in polyethylene bags, was gamma irradiated with doses of 5, 10, 15 and 20 kGy. Control and irradiated samples were stored at 19 ± 4 C and 70 ± 10% RH during 14 months. Microbiological and chemical tests were performed. Samples irradiated with 5 kGy showed two log cycles reduction of sporulated and aerobic mesophilic bacteria, and one log cycle reduction of moulds and yeasts, which fulfilled the microbiological requirements. This situation was maintained throughout the storage period. The irradiation did not significantly affect water content, fat, and total volatile basic nitrogen. Protease and amylase activities decreased with storage time and radiation dose. However, irradiation with 5 and 10 kGy caused no significant alteration on either of these activities as compared with control samples.
LWT - Food Science and Technology, 1993
ABSTRACT
Journal of Food Safety, 1989
The feasibility of inactivating microJora offoreign spices by means of gamma radiation, without c... more The feasibility of inactivating microJora offoreign spices by means of gamma radiation, without causing signijicant organoleptic or chemical changes, was studied. Ground cinnamon, clove, coriander, nutmeg, white and black pepper were irradiated with doses of 7 and I0 kCy' of 6oCo, Chemical analysis were performed so as to verifi the Argentine Alimentary Codex specijications and the effect of radiation on those characteristics by which spices are appreciated, such as color and volatiles. No differences between control and irradiated samples could be found in any of these parameters. Microbiological results showed that aerobic plate count was reduced by 2.5 to 4.0 log cycles with 7 kCy. At 10 kGy survivors could only be found in cinnamon Cinnamon and clove were sensorily evaluated. No differences due to irradia-So, it can be concluded that 10 kGy of gamma radiation sufJlce the purpose (I .7 log cfulg)'. tion were found. of this work. This good market condition was held at least during one year. 'Gray = Gy radiation dose unit. 1 Gy = 1 Joule ikg. 1 kGy = 10' Gy. 'cfuig: colony forming unitsigram.
Journal of Food Safety, 1992
Egg powder was treated with 0, 2, 5 and 10 kGy of gamma radiation at 20C to inactivate Salmonella... more Egg powder was treated with 0, 2, 5 and 10 kGy of gamma radiation at 20C to inactivate Salmonella and to stabilize its microbial load. Microbial, physicochemical and sensory determinations were pe flormed during 4 months of storage to select the optimal radiation dose to attain the objective without signijkantly reducing egg quality. Microbial results show that 2.0 kGy inactivated Salmonella and reduced microbial load to levels below those stipulated by the Argentine regulations. Physicochemicat determinations of egg powder extracts for peroxide number, spectrophotometric measurements in the visible and ultraviolet regions, functional properties on sponge cakes made with egg powder (height, comprssion-relaxation cycle parameters), foam stability and viscosity showed that gamma radiation at the dose of 2 kGy, did not cause signi$cant changes in these parameters. Higher radiation doses (5 and 10 kGy) did increase rancidity, pigment loss and protein chain scission. Sensory determinations performed on egg powder, and on cakes manufactured with it, agreed with the physicochemical results. Ajer 110 storage days, 2 kGy was the most suitable of the tested doses.
Zeitschrift für allgemeine Mikrobiologie, 1980
Zeitschrift für allgemeine Mikrobiologie, 1981
For characterizing the radiation-resistant Acinetobacter strain FO-1 reported in the previous pap... more For characterizing the radiation-resistant Acinetobacter strain FO-1 reported in the previous paper (1980), we investigated the structure of cell wall, the cellular fatty acid composition, and the detailed taxonomic characteristics. The results denoted that the cell division occurred by simple constriction with the formation of a slight septum and the intermediate dense layer between the outer membrane and the plasma membrane was seen, the main components of the cellular fatty acids were oleic acid, palmitic acid, and palmitoleic acid, and that the strain was tolerant to salt and could not produce acids from cellobiose, melibiose, lactose, and ribose.
The Journal of General and Applied Microbiology, 1979
Radiation Physics and Chemistry, 2009
ABSTRACT In the treatment of burns or accidental loss of skin, cadaveric skin allografts provide ... more ABSTRACT In the treatment of burns or accidental loss of skin, cadaveric skin allografts provide an alternative to temporarily cover a wounded area. The skin bank facility is indispensable for burn care. The first human skin bank was established in Argentina in 1989; later, 3 more banks were established.A careful donor selection is carried out according to the national regulation in order to prevent transmissible diseases. As cadaveric human skin is naturally highly contaminated, a final sterilization is necessary to reach a sterility assurance level (SAL) of 10−6.The sterilization dose for 106 batches of processed human skin was determined on the basis of the Code of Practice for the Radiation Sterilization of Tissue Allografts: Requirements for Validation and Routine Control (2004) and ISO 11137-2 (2006). They ranged from 17.6 to 33.4 kGy for bioburdens of >10–162.700 CFU/100 cm2. The presence of Gram negative bacteria was checked for each produced batch. From the analysis of the experimental results, it was observed that the bioburden range was very wide and consequently the estimated sterilization doses too. If this is the case, the determination of a tissue-specific dose per production batch is necessary to achieve a specified requirement of SAL. Otherwise if the dose of 25 kGy is preselected, a standardized method for substantiation of this dose should be done to confirm the radiation sterilization process.
Radiation Physics and Chemistry, 2009
The irradiation treatment of fresh fruits and vegetables for phytosanitary purposes is a satisfac... more The irradiation treatment of fresh fruits and vegetables for phytosanitary purposes is a satisfactory alternative method to others like fumigation and cold and hot treatments. Its use is increasing in several countries, and at present its approval is under revision by the National Regulatory Authorities. To verify the control process, apart from irradiation and dosimetry certificates, National Authorities require complementary evidence to show the efficacy of this treatment, especially when the documentation is not clear. The irradiation of fresh fruits produces single and double fragmentation in the DNA molecule, which can be measured using the microgel electrophoresis of individual cell (comet assay). The purpose of this work was to evaluate if it is possible to identify the irradiated apples for phytosanitary purposes from the others that were not treated. The possibility to estimate the absorbed dose was also evaluated. The methodology was carried out on the cell suspension obtained from irradiated seed cells with incremental doses (100, 200 and 300 Gy). The irradiation treatment for phytosanitary purposes to avoid emergency of codling moth (Cydia pomonella) is 200 Gy. The fragmentation produced in the irradiated samples was proportional with the incremental doses applied. These results show that with this methodology it can be determined if the apple was irradiated or not. This comet assay is a simple, economical and interesting method that can be used, in case of necessity, by the National Authorities.
The fruit fly Bactrocera tau (Walker) is an important quarantine pest that damages fruits and veg... more The fruit fly Bactrocera tau (Walker) is an important quarantine pest that damages fruits and vegetables throughout Asian regions. Host commodities shipped from infested areas should undergo phytosanitary measures to reduce the risk of shipping viable flies. The dose–response tests with 1-d-old eggs and 3-, 5-, 7-, 8-d-old larvae were initiated to determine the most resistant stages in fruits, and the minimum dose for 99.9968% prevention of adult eclosion at 95% confidence level was validated in the confirmatory tests. The results showed that 1) the pupariation rate was not affected by gamma radiation except for eggs and first instars, while the percent of eclosion was reduced significantly in all instars at all radiation dose; 2) the tolerance to radiation increased with increasing age and developmental stage; 3) the estimated dose to 99.9968% preventing adult eclosion from late third instars was 70.9 Gy (95% CL: 65.6–78.2, probit model) and 71.8 Gy (95% CL: 63.0–87.3, logit model)...
Radiation Physics and Chemistry, 2002
Samples of nine different papers in use in the library, including two mould-contaminated papers, ... more Samples of nine different papers in use in the library, including two mould-contaminated papers, were irradiated with 14.4 kGy in a Co-60 facility. Five of the samples were subsequently subjected to an accelerated UV-ageing process. mechanical (tensile energy ...
Radiation Physics and Chemistry, 1997
Dehydrated pancreatin, packed in polyethylene bags, was gamma irradiated with doses of 5, 10, 15 ... more Dehydrated pancreatin, packed in polyethylene bags, was gamma irradiated with doses of 5, 10, 15 and 20 kGy. Control and irradiated samples were stored at 19 ± 4 C and 70 ± 10% RH during 14 months. Microbiological and chemical tests were performed. Samples irradiated with 5 kGy showed two log cycles reduction of sporulated and aerobic mesophilic bacteria, and one log cycle reduction of moulds and yeasts, which fulfilled the microbiological requirements. This situation was maintained throughout the storage period. The irradiation did not significantly affect water content, fat, and total volatile basic nitrogen. Protease and amylase activities decreased with storage time and radiation dose. However, irradiation with 5 and 10 kGy caused no significant alteration on either of these activities as compared with control samples.
LWT - Food Science and Technology, 1993
ABSTRACT
Journal of Food Safety, 1989
The feasibility of inactivating microJora offoreign spices by means of gamma radiation, without c... more The feasibility of inactivating microJora offoreign spices by means of gamma radiation, without causing signijicant organoleptic or chemical changes, was studied. Ground cinnamon, clove, coriander, nutmeg, white and black pepper were irradiated with doses of 7 and I0 kCy' of 6oCo, Chemical analysis were performed so as to verifi the Argentine Alimentary Codex specijications and the effect of radiation on those characteristics by which spices are appreciated, such as color and volatiles. No differences between control and irradiated samples could be found in any of these parameters. Microbiological results showed that aerobic plate count was reduced by 2.5 to 4.0 log cycles with 7 kCy. At 10 kGy survivors could only be found in cinnamon Cinnamon and clove were sensorily evaluated. No differences due to irradia-So, it can be concluded that 10 kGy of gamma radiation sufJlce the purpose (I .7 log cfulg)'. tion were found. of this work. This good market condition was held at least during one year. 'Gray = Gy radiation dose unit. 1 Gy = 1 Joule ikg. 1 kGy = 10' Gy. 'cfuig: colony forming unitsigram.
Journal of Food Safety, 1992
Egg powder was treated with 0, 2, 5 and 10 kGy of gamma radiation at 20C to inactivate Salmonella... more Egg powder was treated with 0, 2, 5 and 10 kGy of gamma radiation at 20C to inactivate Salmonella and to stabilize its microbial load. Microbial, physicochemical and sensory determinations were pe flormed during 4 months of storage to select the optimal radiation dose to attain the objective without signijkantly reducing egg quality. Microbial results show that 2.0 kGy inactivated Salmonella and reduced microbial load to levels below those stipulated by the Argentine regulations. Physicochemicat determinations of egg powder extracts for peroxide number, spectrophotometric measurements in the visible and ultraviolet regions, functional properties on sponge cakes made with egg powder (height, comprssion-relaxation cycle parameters), foam stability and viscosity showed that gamma radiation at the dose of 2 kGy, did not cause signi$cant changes in these parameters. Higher radiation doses (5 and 10 kGy) did increase rancidity, pigment loss and protein chain scission. Sensory determinations performed on egg powder, and on cakes manufactured with it, agreed with the physicochemical results. Ajer 110 storage days, 2 kGy was the most suitable of the tested doses.
Zeitschrift für allgemeine Mikrobiologie, 1980
Zeitschrift für allgemeine Mikrobiologie, 1981
For characterizing the radiation-resistant Acinetobacter strain FO-1 reported in the previous pap... more For characterizing the radiation-resistant Acinetobacter strain FO-1 reported in the previous paper (1980), we investigated the structure of cell wall, the cellular fatty acid composition, and the detailed taxonomic characteristics. The results denoted that the cell division occurred by simple constriction with the formation of a slight septum and the intermediate dense layer between the outer membrane and the plasma membrane was seen, the main components of the cellular fatty acids were oleic acid, palmitic acid, and palmitoleic acid, and that the strain was tolerant to salt and could not produce acids from cellobiose, melibiose, lactose, and ribose.
The Journal of General and Applied Microbiology, 1979
Radiation Physics and Chemistry, 2009
ABSTRACT In the treatment of burns or accidental loss of skin, cadaveric skin allografts provide ... more ABSTRACT In the treatment of burns or accidental loss of skin, cadaveric skin allografts provide an alternative to temporarily cover a wounded area. The skin bank facility is indispensable for burn care. The first human skin bank was established in Argentina in 1989; later, 3 more banks were established.A careful donor selection is carried out according to the national regulation in order to prevent transmissible diseases. As cadaveric human skin is naturally highly contaminated, a final sterilization is necessary to reach a sterility assurance level (SAL) of 10−6.The sterilization dose for 106 batches of processed human skin was determined on the basis of the Code of Practice for the Radiation Sterilization of Tissue Allografts: Requirements for Validation and Routine Control (2004) and ISO 11137-2 (2006). They ranged from 17.6 to 33.4 kGy for bioburdens of >10–162.700 CFU/100 cm2. The presence of Gram negative bacteria was checked for each produced batch. From the analysis of the experimental results, it was observed that the bioburden range was very wide and consequently the estimated sterilization doses too. If this is the case, the determination of a tissue-specific dose per production batch is necessary to achieve a specified requirement of SAL. Otherwise if the dose of 25 kGy is preselected, a standardized method for substantiation of this dose should be done to confirm the radiation sterilization process.
Radiation Physics and Chemistry, 2009
The irradiation treatment of fresh fruits and vegetables for phytosanitary purposes is a satisfac... more The irradiation treatment of fresh fruits and vegetables for phytosanitary purposes is a satisfactory alternative method to others like fumigation and cold and hot treatments. Its use is increasing in several countries, and at present its approval is under revision by the National Regulatory Authorities. To verify the control process, apart from irradiation and dosimetry certificates, National Authorities require complementary evidence to show the efficacy of this treatment, especially when the documentation is not clear. The irradiation of fresh fruits produces single and double fragmentation in the DNA molecule, which can be measured using the microgel electrophoresis of individual cell (comet assay). The purpose of this work was to evaluate if it is possible to identify the irradiated apples for phytosanitary purposes from the others that were not treated. The possibility to estimate the absorbed dose was also evaluated. The methodology was carried out on the cell suspension obtained from irradiated seed cells with incremental doses (100, 200 and 300 Gy). The irradiation treatment for phytosanitary purposes to avoid emergency of codling moth (Cydia pomonella) is 200 Gy. The fragmentation produced in the irradiated samples was proportional with the incremental doses applied. These results show that with this methodology it can be determined if the apple was irradiated or not. This comet assay is a simple, economical and interesting method that can be used, in case of necessity, by the National Authorities.
The fruit fly Bactrocera tau (Walker) is an important quarantine pest that damages fruits and veg... more The fruit fly Bactrocera tau (Walker) is an important quarantine pest that damages fruits and vegetables throughout Asian regions. Host commodities shipped from infested areas should undergo phytosanitary measures to reduce the risk of shipping viable flies. The dose–response tests with 1-d-old eggs and 3-, 5-, 7-, 8-d-old larvae were initiated to determine the most resistant stages in fruits, and the minimum dose for 99.9968% prevention of adult eclosion at 95% confidence level was validated in the confirmatory tests. The results showed that 1) the pupariation rate was not affected by gamma radiation except for eggs and first instars, while the percent of eclosion was reduced significantly in all instars at all radiation dose; 2) the tolerance to radiation increased with increasing age and developmental stage; 3) the estimated dose to 99.9968% preventing adult eclosion from late third instars was 70.9 Gy (95% CL: 65.6–78.2, probit model) and 71.8 Gy (95% CL: 63.0–87.3, logit model)...