Eva Prosecká - Academia.edu (original) (raw)
Papers by Eva Prosecká
The International journal of artificial organs
Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca, 2013
Articular cartilage defects arise due to injury or osteochondral disease such as osteonecrosis or... more Articular cartilage defects arise due to injury or osteochondral disease such as osteonecrosis or osteochondritis dissecans. In adult patients cartilage has minimal ability to repair itself and the lesions develop into degenerative arthritis. Overcoming the low regenerative capacity of the cartilage cells and the Hayflick limit poses a challenge for the therapy of osteochondral defects. Composite scaffolds with appropriate biomechanical properties combined with a suitable blend of proliferation and differentiation factors could be a solution. The aim of this in vitro study was to develop a novel functionalised hydrogel with an integrated drug delivery system stimulating articular cartilage regeneration. Injectable collagen/ hyaluronic acid/fibrin composite hydrogel was mixed with nanofibre-based microparticles. These were loaded with ascorbic acid and dexamethasone. In addition, the effect of thrombocyte-rich solution (TRS) was studied. The gels seeded with mesenchymal stem cells (M...
Physiological research / Academia Scientiarum Bohemoslovaca, 2010
Non-woven textile mesh from polyglycolic acid (PGA) was found as a proper material for chondrocyt... more Non-woven textile mesh from polyglycolic acid (PGA) was found as a proper material for chondrocyte adhesion but worse for their proliferation. Neither hyaluronic acid nor chitosan nor polyvinyl alcohol (PVA) increased chondrocyte adhesion. However, chondrocyte proliferation suffered from acidic byproducts of PGA degradation. However, the addition of PVA and/or chitosan into a wet-laid non-woven textile mesh from PGA improved chondrocyte proliferation seeded in vitro on the PGA-based composite scaffold namely due to a diminished acidification of their microenvironment. This PVA/PGA composite mesh used in combination with a proper hydrogel minimized the negative effect of PGA degradation without dropping positive parameters of the PGA wet-laid non-woven textile mesh. In fact, presence of PVA and/or chitosan in the PGA-based wet-laid non-woven textile mesh even advanced the PGA-based wet-laid non-woven textile mesh for chondrocyte seeding and artificial cartilage production due to a po...
Journal of Biomedical Materials Research Part A, 2014
Key Engineering Materials, 2013
Our aim was to use stereology to quantify the volume fraction of osteocyte lacunes, volume fracti... more Our aim was to use stereology to quantify the volume fraction of osteocyte lacunes, volume fraction of large blood vessels, numerical density of osteocyte lacunes, volume of osteocyte lacunae and bone surface in series of micro-CT images representing samples of spongy and compact bone of human tibia. The spongy bone had a smaller volume fraction of osteocyte lacunes, a greater numerical density of bone lacunes, a smaller volume of the lacunes within the same bone volume and a greater bone surface density when compared to the compact bone. Stereology provided us with data on hierarchical organization of bone structural heterogeneity with reasonable time costs.
International Journal of Pharmaceutics, 2013
The aim of the study was to evaluate the effect of a cell-free hyaluronate/type I collagen/fibrin... more The aim of the study was to evaluate the effect of a cell-free hyaluronate/type I collagen/fibrin composite scaffold containing polyvinyl alcohol (PVA) nanofibers enriched with liposomes, basic fibroblast growth factor (bFGF) and insulin on the regeneration of osteochondral defects.
International Journal of Nanomedicine, 2014
Nanomedicine, 2013
Platelet derivatives serve as an efficient source of natural growth factors. In the current study... more Platelet derivatives serve as an efficient source of natural growth factors. In the current study, α-granules were incorporated into coaxial nanofibers. A nanofiber scaffold containing α-granules was prepared by coaxial electrospinning. The biological potential of the nanofiber scaffold was evaluated in chondrocyte and mesenchymal stem cell cultivation studies. Additionally, the concentration of TGF-β1 was determined. Microscopy studies showed that intact α-granules were incorporated into the coaxial nanofibers. The cultivation tests showed that the novel scaffold stimulated viability and extracellular matrix production of chondrocytes and mesenchymal stem cells. In addition, the concentration of growth factors necessary for the induction of cell proliferation significantly decreased. The system preserved α-granule bioactivity and stimulated cell viability and chondrogenic differentiation of mesenchymal stem cells. Core/shell nanofibers incorporating α-granules are a promising system for tissue engineering, particularly cartilage engineering.
Journal of Biomedicine and Biotechnology, 2012
Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxia... more Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ε-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
Cell Proliferation, 2011
Objectives: The aim of this study was to develop functionalized nanofibres as a simple delivery s... more Objectives: The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cellseeded scaffold implants a one-step intervention. Materials and methods: We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocytecoated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds. Results: Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs. Conclusions: These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.
Cell Proliferation, 2013
Objectives: We prepared 3D poly (ε-caprolactone) (PCL) nanofibrous scaffolds and tested them for ... more Objectives: We prepared 3D poly (ε-caprolactone) (PCL) nanofibrous scaffolds and tested them for the seeding, proliferation, differentiation, and migration of mesenchymal stem cell (MSCs).
Cell Adhesion & Migration, 2013
Cell infiltration is a critical parameter for the successful development of 3D matrices for tissu... more Cell infiltration is a critical parameter for the successful development of 3D matrices for tissue engineering. Application of electrospun nanofibers in tissue engineering has recently attracted much attention. Notwithstanding several of their advantages, small pore size and small thickness of the electrospun layer limit their application for development of 3D scaffolds. Several methods for the pore size and/or electrospun layer thickness increase have been recently developed. Nevertheless, tissue engineering still needs emerging of either novel nanofiber-enriched composites or new techniques for 3D nanofiber fabrication. Forcespinning(®) seems to be a promising alternative. The potential of the Forcespinning(®) method is illustrated in preliminary experiment with mesenchymal stem cells.
ASAIO Journal, 2010
Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) w... more Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) were found to be suitable scaffolds for chondrocyte seeding and pig knee cartilage regeneration. Collagen in the hydrogels is not necessary for the formation of biomechanically stable tissue. Regenerated cartilage showed very good biomechanical and histological properties only 6 months after implantation. Notably, the quality of the healing process was dependent on the initial chondrocyte concentration of the scaffolds. These experiments were performed according to good laboratory practice (GLP).
International journal of nanomedicine, 2015
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients ... more Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thromboc...
The International journal of artificial organs
Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca, 2013
Articular cartilage defects arise due to injury or osteochondral disease such as osteonecrosis or... more Articular cartilage defects arise due to injury or osteochondral disease such as osteonecrosis or osteochondritis dissecans. In adult patients cartilage has minimal ability to repair itself and the lesions develop into degenerative arthritis. Overcoming the low regenerative capacity of the cartilage cells and the Hayflick limit poses a challenge for the therapy of osteochondral defects. Composite scaffolds with appropriate biomechanical properties combined with a suitable blend of proliferation and differentiation factors could be a solution. The aim of this in vitro study was to develop a novel functionalised hydrogel with an integrated drug delivery system stimulating articular cartilage regeneration. Injectable collagen/ hyaluronic acid/fibrin composite hydrogel was mixed with nanofibre-based microparticles. These were loaded with ascorbic acid and dexamethasone. In addition, the effect of thrombocyte-rich solution (TRS) was studied. The gels seeded with mesenchymal stem cells (M...
Physiological research / Academia Scientiarum Bohemoslovaca, 2010
Non-woven textile mesh from polyglycolic acid (PGA) was found as a proper material for chondrocyt... more Non-woven textile mesh from polyglycolic acid (PGA) was found as a proper material for chondrocyte adhesion but worse for their proliferation. Neither hyaluronic acid nor chitosan nor polyvinyl alcohol (PVA) increased chondrocyte adhesion. However, chondrocyte proliferation suffered from acidic byproducts of PGA degradation. However, the addition of PVA and/or chitosan into a wet-laid non-woven textile mesh from PGA improved chondrocyte proliferation seeded in vitro on the PGA-based composite scaffold namely due to a diminished acidification of their microenvironment. This PVA/PGA composite mesh used in combination with a proper hydrogel minimized the negative effect of PGA degradation without dropping positive parameters of the PGA wet-laid non-woven textile mesh. In fact, presence of PVA and/or chitosan in the PGA-based wet-laid non-woven textile mesh even advanced the PGA-based wet-laid non-woven textile mesh for chondrocyte seeding and artificial cartilage production due to a po...
Journal of Biomedical Materials Research Part A, 2014
Key Engineering Materials, 2013
Our aim was to use stereology to quantify the volume fraction of osteocyte lacunes, volume fracti... more Our aim was to use stereology to quantify the volume fraction of osteocyte lacunes, volume fraction of large blood vessels, numerical density of osteocyte lacunes, volume of osteocyte lacunae and bone surface in series of micro-CT images representing samples of spongy and compact bone of human tibia. The spongy bone had a smaller volume fraction of osteocyte lacunes, a greater numerical density of bone lacunes, a smaller volume of the lacunes within the same bone volume and a greater bone surface density when compared to the compact bone. Stereology provided us with data on hierarchical organization of bone structural heterogeneity with reasonable time costs.
International Journal of Pharmaceutics, 2013
The aim of the study was to evaluate the effect of a cell-free hyaluronate/type I collagen/fibrin... more The aim of the study was to evaluate the effect of a cell-free hyaluronate/type I collagen/fibrin composite scaffold containing polyvinyl alcohol (PVA) nanofibers enriched with liposomes, basic fibroblast growth factor (bFGF) and insulin on the regeneration of osteochondral defects.
International Journal of Nanomedicine, 2014
Nanomedicine, 2013
Platelet derivatives serve as an efficient source of natural growth factors. In the current study... more Platelet derivatives serve as an efficient source of natural growth factors. In the current study, α-granules were incorporated into coaxial nanofibers. A nanofiber scaffold containing α-granules was prepared by coaxial electrospinning. The biological potential of the nanofiber scaffold was evaluated in chondrocyte and mesenchymal stem cell cultivation studies. Additionally, the concentration of TGF-β1 was determined. Microscopy studies showed that intact α-granules were incorporated into the coaxial nanofibers. The cultivation tests showed that the novel scaffold stimulated viability and extracellular matrix production of chondrocytes and mesenchymal stem cells. In addition, the concentration of growth factors necessary for the induction of cell proliferation significantly decreased. The system preserved α-granule bioactivity and stimulated cell viability and chondrogenic differentiation of mesenchymal stem cells. Core/shell nanofibers incorporating α-granules are a promising system for tissue engineering, particularly cartilage engineering.
Journal of Biomedicine and Biotechnology, 2012
Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxia... more Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ε-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
Cell Proliferation, 2011
Objectives: The aim of this study was to develop functionalized nanofibres as a simple delivery s... more Objectives: The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cellseeded scaffold implants a one-step intervention. Materials and methods: We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocytecoated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds. Results: Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs. Conclusions: These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.
Cell Proliferation, 2013
Objectives: We prepared 3D poly (ε-caprolactone) (PCL) nanofibrous scaffolds and tested them for ... more Objectives: We prepared 3D poly (ε-caprolactone) (PCL) nanofibrous scaffolds and tested them for the seeding, proliferation, differentiation, and migration of mesenchymal stem cell (MSCs).
Cell Adhesion & Migration, 2013
Cell infiltration is a critical parameter for the successful development of 3D matrices for tissu... more Cell infiltration is a critical parameter for the successful development of 3D matrices for tissue engineering. Application of electrospun nanofibers in tissue engineering has recently attracted much attention. Notwithstanding several of their advantages, small pore size and small thickness of the electrospun layer limit their application for development of 3D scaffolds. Several methods for the pore size and/or electrospun layer thickness increase have been recently developed. Nevertheless, tissue engineering still needs emerging of either novel nanofiber-enriched composites or new techniques for 3D nanofiber fabrication. Forcespinning(®) seems to be a promising alternative. The potential of the Forcespinning(®) method is illustrated in preliminary experiment with mesenchymal stem cells.
ASAIO Journal, 2010
Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) w... more Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) were found to be suitable scaffolds for chondrocyte seeding and pig knee cartilage regeneration. Collagen in the hydrogels is not necessary for the formation of biomechanically stable tissue. Regenerated cartilage showed very good biomechanical and histological properties only 6 months after implantation. Notably, the quality of the healing process was dependent on the initial chondrocyte concentration of the scaffolds. These experiments were performed according to good laboratory practice (GLP).
International journal of nanomedicine, 2015
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients ... more Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thromboc...