Evgeny Petrov - Academia.edu (original) (raw)
Papers by Evgeny Petrov
Medical Gas Research, 2022
Buffer solution was prepared by dissolving 3.2 g of ammonium dihydrophosphate (JSC VEKTON, St. Pe... more Buffer solution was prepared by dissolving 3.2 g of ammonium dihydrophosphate (JSC VEKTON, St. Petersburg, Russia) and 250 μL of concentrated phosphoric acid in 1 L of water. The mobile phase was prepared by mixing 980 mL of buffer solution and 20 mL of acetonitrile (PanReac AppliChem GmbH, Darmstadt, Germany). Flow rate was 1.0 mL/min with isocratic elution. The column temperature was set to 30°C. The detection wavelength was set to 210 nm. Injected volume was 20 µL. Standard solution and calibration solutions were prepared by dissolving a dry sample (niacinamide; DSM Nutritional Products Ltd., Basel, Switzerland) of the standard in deionized water. Chromatography for xenon A Chromatec-Crystall 5000.1 gas chromatograph (Chromatec, Yoshkar-Ola, Russia) with a thermal conductivity detector was used for the analysis. Sample with xenon (Akela
The magnetic field of the Earth has for long been known to influence the behaviour and orientatio... more The magnetic field of the Earth has for long been known to influence the behaviour and orientation of a variety of living organisms. Experimental studies of the magnetic sense have however, been impaired by the lack of a plausible cellular and/or molecular mechanism providing meaningful explanation for detection of magnetic fields by these organisms. Recently, mechanosensitive (MS) ion channels have been implied to play a role in magnetoreception. In this study we have investigated the effect of static magnetic fields (SMFs) of moderate intensity on the activity and gadolinium block of MscL, the bacterial MS channel of large conductance, which has served as a model channel to study the basic physical principles of mechanosensory transduction in living cells. In addition to showing that direct application of the magnetic field decreased the activity of the MscL channel our study demonstrates for the first time that SMFs can reverse the effect of gadolinium, a well known blocker of MS channels. The results of our study are consistent with a notion that (i) the effects of SMFs on the MscL channels may result from changes in physical properties of the lipid bilayer due to diamagnetic anisotropy of phospholipid molecules and consequently (ii) cooperative superdiamagnetism of phospholipid molecules under influence of SMFs could cause displacement of Gd3+ ions from the membrane bilayer and thus remove the MscL channel block. Supported by the Australian Research Council.
Biophysical Journal, Apr 1, 2013
Based on sequence similarity, the sp7 gene product, MscSP, of the sulfur-compound-decomposing Gra... more Based on sequence similarity, the sp7 gene product, MscSP, of the sulfur-compound-decomposing Gram-negative marine bacterium Silicibacter pomeroyi belongs to the family of MscS-type mechanosensitive channels. To investigate MscSP channel properties, we measured its response to membrane tension using the patch-clamp technique on either a heterologous expression system using giant spheroplasts of MJF465 Escherichia coli strain (devoid of mechanosensitive channels MscL, MscS, and MscK), or on purified MscSP protein reconstituted in azolectin liposomes. These experiments showed typical pressure-dependent gating properties of a stretch-activated channel with a current/voltage plot indicating a rectifying behavior and weak preference for anions similar to the MscS channel of E. coli. However, the MscSP channel exhibited functional differences with respect to conductance and desensitization behavior, with the most striking difference between the two channels being the lack of inactivation in MscSP compared with MscS. This seems to result from the fact that although MscSP has a Gly in an equivalent position to MscS (G113), a position that is critical for inactivation, MscSP has a Glu residue instead of an Asn in a position that was recently shown to allosterically influence MscS inactivation, N117. To our knowledge, this study describes the first electrophysiological characterization of an MscS-like channel from a marine bacterium belonging to sulfur-degrading a-proteobacteria.
MATEC Web of Conferences, 2016
We have developed an agent for external use to deliver xenon into the skin. Using the model of sk... more We have developed an agent for external use to deliver xenon into the skin. Using the model of skin wound we have studied the effect of xenon on the process of wound healing in rats. It has been shown that the application of xenon-containing cream on wound surface significantly accelerates the healing process as compared to control animals where cream without xenon has been applied.
MATEC Web of Conferences, 2016
This study is aimed to describe the technology of making xenoncontaining drinking water and its i... more This study is aimed to describe the technology of making xenoncontaining drinking water and its impact on health and psychophysiological characteristics of 26 volunteers after regular admission. Testing conducted after 2 months of daily use such water showed significant improvement of emotional state and health of volunteers. This technology allows obtaining a large amount of drinking water saturated with xenon, which can be used in therapeutic and prophylactic purposes.
Journal of Neurophysiology, 2010
The enzyme nitric oxide (NO) synthase, that produces the signaling molecule NO, has been identifi... more The enzyme nitric oxide (NO) synthase, that produces the signaling molecule NO, has been identified in several cell types in the inner ear. However, it is unclear whether a measurable quantity of NO is released in the inner ear to confer specific functions. Indeed, the functional significance of NO and the elementary cellular mechanism thereof are most uncertain. Here, we demonstrate that the sensory epithelia of the frog saccule release NO and explore its release mechanisms by using self-referencing NO-selective electrodes. Additionally, we investigated the functional effects of NO on electrical properties of hair cells and determined their underlying cellular mechanism. We show detectable amounts of NO are released by hair cells (>50 nM). Furthermore, a hair-cell efferent modulator acetylcholine produces at least a threefold increase in NO release. NO not only attenuated the baseline membrane oscillations but it also increased the magnitude of current required to generate the c...
Bulletin of Experimental Biology and Medicine, 1995
The contribution of calmodulin and protein kinase C to the regulation of epithelial relaxing fact... more The contribution of calmodulin and protein kinase C to the regulation of epithelial relaxing factor production by the tracheal epithelium and the role of adenylate and guanylate cyclase in the realization of the effect of this factor on airway smooth muscles are studied by the mechanographic method with cascade perfusion. Calmodulin and protein kinase C are shown to participate in the production of relaxing factor by epitheliocytes, guanylate cyclase being the principal target in exposure of smooth muscles to epithelial relaxing factor.
PloS one, 2018
Xenon is frequently used as a general anesthetic in humans, but the mechanism remains an issue of... more Xenon is frequently used as a general anesthetic in humans, but the mechanism remains an issue of debate. While for some membrane proteins, a direct interaction of xenon with the protein has been shown to be the inhibitory mechanism, other membrane protein functions could be affected by changes of membrane properties due to partitioning of the gas into the lipid bilayer. Here, the effect of xenon on a mechanosensitive ion channel and a copper ion-translocating ATPase was compared under different conditions. Xenon inhibited spontaneous gating of the Escherichia coli mechano-sensitive mutant channel MscL-G22E, as shown by patch-clamp recording techniques. Under high hydrostatic pressure, MscL-inhibition was reversed. Similarly, the activity of the Enterococcus hirae CopB copper ATPase, reconstituted into proteoliposomes, was inhibited by xenon. However, the CopB ATPase activity was also inhibited by xenon when CopB was in a solubilized state. These findings suggest that xenon acts by ...
European Biophysics Journal, 2007
The magnetic field of the Earth has for long been known to influence the behaviour and orientatio... more The magnetic field of the Earth has for long been known to influence the behaviour and orientation of a variety of living organisms. Experimental studies of the magnetic sense have, however, been impaired by the lack of a plausible cellular and/or molecular mechanism providing meaningful explanation for detection of magnetic fields by these organisms. Recently, mechanosensitive (MS) ion channels have been implied to play a role in magnetoreception. In this study we have investigated the effect of static magnetic fields (SMFs) of moderate intensity on the activity and gadolinium block of MscL, the bacterial MS channel of large conductance, which has served as a model channel to study the basic physical principles of mechanosensory transduction in living cells. In addition to showing that direct application of the magnetic field decreased the activity of the MscL channel, our study demonstrates for the first time that SMFs can reverse the effect of gadolinium, a well-known blocker of MS channels. The results of our study are consistent with a notion that (1) the effects of SMFs on the MscL channels may result from changes in physical properties of the lipid bilayer due to diamagnetic anisotropy of phospholipid molecules and consequently (2) cooperative superdiamagnetism of phospholipid molecules under influence of SMFs could cause displacement of Gd 3+ ions from the membrane bilayer and thus remove the MscL channel block.
European Biophysics Journal, 2005
The mechanosensitive channel of large conductance (MscL) plays an important role in the survival ... more The mechanosensitive channel of large conductance (MscL) plays an important role in the survival of bacterial cells to hypo-osmotic shock. This channel has been extensively studied and its sequence, structure and electrophysiological characteristics are well known.
Antioxidants & Redox Signaling, 2014
Significance: Sensations of touch and hearing are manifestations of mechanical contact and air pr... more Significance: Sensations of touch and hearing are manifestations of mechanical contact and air pressure acting on touch receptors and hair cells of the inner ear, respectively. In bacteria, osmotic pressure exerts a significant mechanical force on their cellular membrane. Bacteria have evolved mechanosensitive (MS) channels to cope with excessive turgor pressure resulting from a hypo-osmotic shock. MS channel opening allows the expulsion of osmolytes and water, thereby restoring normal cellular turgor and preventing cell lysis. Recent Advances: As biological force-sensing systems, MS channels have been identified as the best examples of membrane proteins coupling molecular dynamics to cellular mechanics. The bacterial MS channel of large conductance (MscL) and MS channel of small conductance (MscS) have been subjected to extensive biophysical, biochemical, genetic, and structural analyses. These studies have established MscL and MscS as model systems for mechanosensory transduction. Critical Issues: In recent years, MS ion channels in mammalian cells have moved into focus of mechanotransduction research, accompanied by an increased awareness of the role they may play in the pathophysiology of diseases, including cardiac hypertrophy, muscular dystrophy, or Xerocytosis. Future Directions: A recent exciting development includes the molecular identification of Piezo proteins, which function as nonselective cation channels in mechanosensory transduction associated with senses of touch and pain. Since research on Piezo channels is very young, applying lessons learned from studies of bacterial MS channels to establishing the mechanism by which the Piezo channels are mechanically activated remains one of the future challenges toward a better understanding of the role that MS channels play in mechanobiology. Antioxid. Redox
Biophysical Journal, 2011
protoplast formation increases the probability of mechanosensitive activity in a patch. Furthermo... more protoplast formation increases the probability of mechanosensitive activity in a patch. Furthermore, a new mechanosensitive channel conductance (~1nS) is revealed in~40% of patches. Tests so far indicate that 0.5M KCl incubations do not cause the same effect. Further analysis of these novel activities will enhance our knowledge of mechanosensitive channels in bacteria.
Fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk
The rat trachea epithelium was found to affect a catecholamine-induced relaxation of the trachea ... more The rat trachea epithelium was found to affect a catecholamine-induced relaxation of the trachea smooth muscles, to release a relaxing factor, the producing of which depends on calcium and is regulated by calmoduline and proteinkinase C. The epithelium-induced relaxation seems to depend on the animal age.
Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk, 1997
The rat aorta smooth muscles were contracted and depolarised with high potassium or phenylephrin ... more The rat aorta smooth muscles were contracted and depolarised with high potassium or phenylephrin solution which was followed by a relaxation and repolarisation after sodium nitroprusside administration. The latter effect was decreased az a combined action of phenylephrin and high potassium solution. Nitroprusside seems to act through a cGMP-dependent potassium transient activation of the smooth muscle cell membrane.
Medical Gas Research, 2022
Buffer solution was prepared by dissolving 3.2 g of ammonium dihydrophosphate (JSC VEKTON, St. Pe... more Buffer solution was prepared by dissolving 3.2 g of ammonium dihydrophosphate (JSC VEKTON, St. Petersburg, Russia) and 250 μL of concentrated phosphoric acid in 1 L of water. The mobile phase was prepared by mixing 980 mL of buffer solution and 20 mL of acetonitrile (PanReac AppliChem GmbH, Darmstadt, Germany). Flow rate was 1.0 mL/min with isocratic elution. The column temperature was set to 30°C. The detection wavelength was set to 210 nm. Injected volume was 20 µL. Standard solution and calibration solutions were prepared by dissolving a dry sample (niacinamide; DSM Nutritional Products Ltd., Basel, Switzerland) of the standard in deionized water. Chromatography for xenon A Chromatec-Crystall 5000.1 gas chromatograph (Chromatec, Yoshkar-Ola, Russia) with a thermal conductivity detector was used for the analysis. Sample with xenon (Akela
The magnetic field of the Earth has for long been known to influence the behaviour and orientatio... more The magnetic field of the Earth has for long been known to influence the behaviour and orientation of a variety of living organisms. Experimental studies of the magnetic sense have however, been impaired by the lack of a plausible cellular and/or molecular mechanism providing meaningful explanation for detection of magnetic fields by these organisms. Recently, mechanosensitive (MS) ion channels have been implied to play a role in magnetoreception. In this study we have investigated the effect of static magnetic fields (SMFs) of moderate intensity on the activity and gadolinium block of MscL, the bacterial MS channel of large conductance, which has served as a model channel to study the basic physical principles of mechanosensory transduction in living cells. In addition to showing that direct application of the magnetic field decreased the activity of the MscL channel our study demonstrates for the first time that SMFs can reverse the effect of gadolinium, a well known blocker of MS channels. The results of our study are consistent with a notion that (i) the effects of SMFs on the MscL channels may result from changes in physical properties of the lipid bilayer due to diamagnetic anisotropy of phospholipid molecules and consequently (ii) cooperative superdiamagnetism of phospholipid molecules under influence of SMFs could cause displacement of Gd3+ ions from the membrane bilayer and thus remove the MscL channel block. Supported by the Australian Research Council.
Biophysical Journal, Apr 1, 2013
Based on sequence similarity, the sp7 gene product, MscSP, of the sulfur-compound-decomposing Gra... more Based on sequence similarity, the sp7 gene product, MscSP, of the sulfur-compound-decomposing Gram-negative marine bacterium Silicibacter pomeroyi belongs to the family of MscS-type mechanosensitive channels. To investigate MscSP channel properties, we measured its response to membrane tension using the patch-clamp technique on either a heterologous expression system using giant spheroplasts of MJF465 Escherichia coli strain (devoid of mechanosensitive channels MscL, MscS, and MscK), or on purified MscSP protein reconstituted in azolectin liposomes. These experiments showed typical pressure-dependent gating properties of a stretch-activated channel with a current/voltage plot indicating a rectifying behavior and weak preference for anions similar to the MscS channel of E. coli. However, the MscSP channel exhibited functional differences with respect to conductance and desensitization behavior, with the most striking difference between the two channels being the lack of inactivation in MscSP compared with MscS. This seems to result from the fact that although MscSP has a Gly in an equivalent position to MscS (G113), a position that is critical for inactivation, MscSP has a Glu residue instead of an Asn in a position that was recently shown to allosterically influence MscS inactivation, N117. To our knowledge, this study describes the first electrophysiological characterization of an MscS-like channel from a marine bacterium belonging to sulfur-degrading a-proteobacteria.
MATEC Web of Conferences, 2016
We have developed an agent for external use to deliver xenon into the skin. Using the model of sk... more We have developed an agent for external use to deliver xenon into the skin. Using the model of skin wound we have studied the effect of xenon on the process of wound healing in rats. It has been shown that the application of xenon-containing cream on wound surface significantly accelerates the healing process as compared to control animals where cream without xenon has been applied.
MATEC Web of Conferences, 2016
This study is aimed to describe the technology of making xenoncontaining drinking water and its i... more This study is aimed to describe the technology of making xenoncontaining drinking water and its impact on health and psychophysiological characteristics of 26 volunteers after regular admission. Testing conducted after 2 months of daily use such water showed significant improvement of emotional state and health of volunteers. This technology allows obtaining a large amount of drinking water saturated with xenon, which can be used in therapeutic and prophylactic purposes.
Journal of Neurophysiology, 2010
The enzyme nitric oxide (NO) synthase, that produces the signaling molecule NO, has been identifi... more The enzyme nitric oxide (NO) synthase, that produces the signaling molecule NO, has been identified in several cell types in the inner ear. However, it is unclear whether a measurable quantity of NO is released in the inner ear to confer specific functions. Indeed, the functional significance of NO and the elementary cellular mechanism thereof are most uncertain. Here, we demonstrate that the sensory epithelia of the frog saccule release NO and explore its release mechanisms by using self-referencing NO-selective electrodes. Additionally, we investigated the functional effects of NO on electrical properties of hair cells and determined their underlying cellular mechanism. We show detectable amounts of NO are released by hair cells (>50 nM). Furthermore, a hair-cell efferent modulator acetylcholine produces at least a threefold increase in NO release. NO not only attenuated the baseline membrane oscillations but it also increased the magnitude of current required to generate the c...
Bulletin of Experimental Biology and Medicine, 1995
The contribution of calmodulin and protein kinase C to the regulation of epithelial relaxing fact... more The contribution of calmodulin and protein kinase C to the regulation of epithelial relaxing factor production by the tracheal epithelium and the role of adenylate and guanylate cyclase in the realization of the effect of this factor on airway smooth muscles are studied by the mechanographic method with cascade perfusion. Calmodulin and protein kinase C are shown to participate in the production of relaxing factor by epitheliocytes, guanylate cyclase being the principal target in exposure of smooth muscles to epithelial relaxing factor.
PloS one, 2018
Xenon is frequently used as a general anesthetic in humans, but the mechanism remains an issue of... more Xenon is frequently used as a general anesthetic in humans, but the mechanism remains an issue of debate. While for some membrane proteins, a direct interaction of xenon with the protein has been shown to be the inhibitory mechanism, other membrane protein functions could be affected by changes of membrane properties due to partitioning of the gas into the lipid bilayer. Here, the effect of xenon on a mechanosensitive ion channel and a copper ion-translocating ATPase was compared under different conditions. Xenon inhibited spontaneous gating of the Escherichia coli mechano-sensitive mutant channel MscL-G22E, as shown by patch-clamp recording techniques. Under high hydrostatic pressure, MscL-inhibition was reversed. Similarly, the activity of the Enterococcus hirae CopB copper ATPase, reconstituted into proteoliposomes, was inhibited by xenon. However, the CopB ATPase activity was also inhibited by xenon when CopB was in a solubilized state. These findings suggest that xenon acts by ...
European Biophysics Journal, 2007
The magnetic field of the Earth has for long been known to influence the behaviour and orientatio... more The magnetic field of the Earth has for long been known to influence the behaviour and orientation of a variety of living organisms. Experimental studies of the magnetic sense have, however, been impaired by the lack of a plausible cellular and/or molecular mechanism providing meaningful explanation for detection of magnetic fields by these organisms. Recently, mechanosensitive (MS) ion channels have been implied to play a role in magnetoreception. In this study we have investigated the effect of static magnetic fields (SMFs) of moderate intensity on the activity and gadolinium block of MscL, the bacterial MS channel of large conductance, which has served as a model channel to study the basic physical principles of mechanosensory transduction in living cells. In addition to showing that direct application of the magnetic field decreased the activity of the MscL channel, our study demonstrates for the first time that SMFs can reverse the effect of gadolinium, a well-known blocker of MS channels. The results of our study are consistent with a notion that (1) the effects of SMFs on the MscL channels may result from changes in physical properties of the lipid bilayer due to diamagnetic anisotropy of phospholipid molecules and consequently (2) cooperative superdiamagnetism of phospholipid molecules under influence of SMFs could cause displacement of Gd 3+ ions from the membrane bilayer and thus remove the MscL channel block.
European Biophysics Journal, 2005
The mechanosensitive channel of large conductance (MscL) plays an important role in the survival ... more The mechanosensitive channel of large conductance (MscL) plays an important role in the survival of bacterial cells to hypo-osmotic shock. This channel has been extensively studied and its sequence, structure and electrophysiological characteristics are well known.
Antioxidants & Redox Signaling, 2014
Significance: Sensations of touch and hearing are manifestations of mechanical contact and air pr... more Significance: Sensations of touch and hearing are manifestations of mechanical contact and air pressure acting on touch receptors and hair cells of the inner ear, respectively. In bacteria, osmotic pressure exerts a significant mechanical force on their cellular membrane. Bacteria have evolved mechanosensitive (MS) channels to cope with excessive turgor pressure resulting from a hypo-osmotic shock. MS channel opening allows the expulsion of osmolytes and water, thereby restoring normal cellular turgor and preventing cell lysis. Recent Advances: As biological force-sensing systems, MS channels have been identified as the best examples of membrane proteins coupling molecular dynamics to cellular mechanics. The bacterial MS channel of large conductance (MscL) and MS channel of small conductance (MscS) have been subjected to extensive biophysical, biochemical, genetic, and structural analyses. These studies have established MscL and MscS as model systems for mechanosensory transduction. Critical Issues: In recent years, MS ion channels in mammalian cells have moved into focus of mechanotransduction research, accompanied by an increased awareness of the role they may play in the pathophysiology of diseases, including cardiac hypertrophy, muscular dystrophy, or Xerocytosis. Future Directions: A recent exciting development includes the molecular identification of Piezo proteins, which function as nonselective cation channels in mechanosensory transduction associated with senses of touch and pain. Since research on Piezo channels is very young, applying lessons learned from studies of bacterial MS channels to establishing the mechanism by which the Piezo channels are mechanically activated remains one of the future challenges toward a better understanding of the role that MS channels play in mechanobiology. Antioxid. Redox
Biophysical Journal, 2011
protoplast formation increases the probability of mechanosensitive activity in a patch. Furthermo... more protoplast formation increases the probability of mechanosensitive activity in a patch. Furthermore, a new mechanosensitive channel conductance (~1nS) is revealed in~40% of patches. Tests so far indicate that 0.5M KCl incubations do not cause the same effect. Further analysis of these novel activities will enhance our knowledge of mechanosensitive channels in bacteria.
Fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk
The rat trachea epithelium was found to affect a catecholamine-induced relaxation of the trachea ... more The rat trachea epithelium was found to affect a catecholamine-induced relaxation of the trachea smooth muscles, to release a relaxing factor, the producing of which depends on calcium and is regulated by calmoduline and proteinkinase C. The epithelium-induced relaxation seems to depend on the animal age.
Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk, 1997
The rat aorta smooth muscles were contracted and depolarised with high potassium or phenylephrin ... more The rat aorta smooth muscles were contracted and depolarised with high potassium or phenylephrin solution which was followed by a relaxation and repolarisation after sodium nitroprusside administration. The latter effect was decreased az a combined action of phenylephrin and high potassium solution. Nitroprusside seems to act through a cGMP-dependent potassium transient activation of the smooth muscle cell membrane.