Ezdehar Ali - Academia.edu (original) (raw)

Papers by Ezdehar Ali

Research paper thumbnail of A New Sperm Preparation Technique by Combination of Density Gradient Centrifugation and Glass Wool Filtration Techniques Versus Each One Alone for Infertile Men with Asthenozoospermia

International Journal of Advanced Research, 2016

Background:-A variety of techniques have been developed to separate motile and morphologically no... more Background:-A variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive techniques. Objectives:-To compare the asthenozoospermic semen outcomes of three in vitro sperm activation (ISA) techniques and evaluate the efficacy, namely; Density gradient centrifugation (DGC) technique, Glass wool filtration technique and Max pure technique(combination of DGC and GWF techniques). Methods:-Forty three infertile men with asthenozoospermia were involved in this study. Sperm parameters assessed according to WHO (2010 and 1999). Post-activation of each sample divided into three aliquots, the first one using DGC, the second using GWF, and last one using Max pure technique. Results:-A significant increase (P<0.05) of sperm motility, progressive sperm motility and normal sperm morphology when using Max pure technique as compared to DGC and GWF techniques. Also, there was a significant increase (P<0.05) for the same parameters when using DGC technique as compared to the GWF technique. Conclusions:-Using Max pure technique for semen sample with decreased in the sperm motility were superior to that of DGC and GWF techniques.

Research paper thumbnail of ISSN 2320-5407 International Journal of Advanced Research (2016), Volume 4, Issue 4, 1112-1115

Ula M. R. Al-Kawaz. Background:A variety of techniques have been developed to separate motile and... more Ula M. R. Al-Kawaz. Background:A variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive techniques. Objectives:To compare the asthenozoospermic semen outcomes of three in vitro sperm activation (ISA) techniques and evaluate the efficacy, namely; Density gradient centrifugation (DGC) technique, Glass wool filtration technique and Max pure technique(combination of DGC and GWF techniques). Methods:Forty three infertile men with asthenozoospermia were involved in this study. Sperm parameters assessed according to WHO (2010 and 1999). Postactivation of each sample divided into three aliquots, the first one using DGC, the second using GWF, and last one using Max pure technique. Results:A significant increase (P<0.05) of sperm motility, progressive sperm motility and normal sperm morphology when using Max pure technique as compared to DGC and GWF techniques....

Research paper thumbnail of New Approach of Hyaluronic Acid Bound Spermatozoa-ICSI in Iraqi Infertile Patients

Indian Journal of Public Health Research & Development

Research paper thumbnail of The New Approach of Hyaluronic Binding Assay in Relevance to Sperm Activation by Direct Swim-up Technique in Iraqi Infertile Men

Iraqi Journal of Embryos and Infertility Researches

Hyaluronic acid (HA) is a polysaccharide, that composed in the extracellular matrix of the cumulu... more Hyaluronic acid (HA) is a polysaccharide, that composed in the extracellular matrix of the cumulus cells, and considered to take function in choosing mature spermatozoa. The Sperm-hyaluronic binding assay (HBA) is significant diagnostic instrument for suspected male infertility when examining the semen. The HBA slide supplies the ratio of mature binding sperm in the specimen. To evaluate sperm parameters, and to evaluate the (HBA Score percentage (%) before and after activation. Twenty-five infertile couples a sharer in this current study through their attendance to the Al Nahrain University. Males undergoing seminal fluid analysis were done according to (WHO 2010, and WHO 1999). Sperm parameters assessed and calculate the HBA Score% pre-and-post-direct swim-up technique, the results were statistically tested. In Post-activation there was considerable increment noticed in the progressive motility and morphology of spermatozoa, likewise significant improvement in the (HBA score %). Furthermore, considerable reduction in the concentration, agglutination of spermatozoa and round cells count, when compared to pre-activation. The mean of binding of spermatozoa to hyaluronan pre-activation (direct swim-up technique) was less than normal limit for normozoospermic males. However, it significantly improves post-activation.

Research paper thumbnail of A NEW SPERM PREPARATION TECHNIQUE BY COMBINATION OF DENSITY GRADIENT CENTRIFUGATION AND GLASS WOOL FILTRATION TECHNIQUES VERSUS EACH ONE ALONE FOR INFERTILE MEN WITH ASTHENOZOOSPERMIA

Background:-A variety of techniques have been developed to separate motile and morphologically no... more Background:-A variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive techniques. Objectives:-To compare the asthenozoospermic semen outcomes of three in vitro sperm activation (ISA) techniques and evaluate the efficacy, namely; Density gradient centrifugation (DGC) technique, Glass wool filtration technique and Max pure technique(combination of DGC and GWF techniques). Methods:-Forty three infertile men with asthenozoospermia were involved in this study. Sperm parameters assessed according to WHO (2010 and 1999). Post-activation of each sample divided into three aliquots, the first one using DGC, the second using GWF, and last one using Max pure technique. Results:-A significant increase (P<0.05) of sperm motility, progressive sperm motility and normal sperm morphology when using Max pure technique as compared to DGC and GWF techniques. Also, there was a significant increase (P<0.05) for the same parameters when using DGC technique as compared to the GWF technique. Conclusions:-Using Max pure technique for semen sample with decreased in the sperm motility were superior to that of DGC and GWF techniques. Copy Right, IJAR, 2016,. All rights reserved.

Research paper thumbnail of A New Sperm Preparation Technique by Combination of Density Gradient Centrifugation and Glass Wool Filtration Techniques Versus Each One Alone for Infertile Men with Asthenozoospermia

International Journal of Advanced Research, 2016

Background:-A variety of techniques have been developed to separate motile and morphologically no... more Background:-A variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive techniques. Objectives:-To compare the asthenozoospermic semen outcomes of three in vitro sperm activation (ISA) techniques and evaluate the efficacy, namely; Density gradient centrifugation (DGC) technique, Glass wool filtration technique and Max pure technique(combination of DGC and GWF techniques). Methods:-Forty three infertile men with asthenozoospermia were involved in this study. Sperm parameters assessed according to WHO (2010 and 1999). Post-activation of each sample divided into three aliquots, the first one using DGC, the second using GWF, and last one using Max pure technique. Results:-A significant increase (P<0.05) of sperm motility, progressive sperm motility and normal sperm morphology when using Max pure technique as compared to DGC and GWF techniques. Also, there was a significant increase (P<0.05) for the same parameters when using DGC technique as compared to the GWF technique. Conclusions:-Using Max pure technique for semen sample with decreased in the sperm motility were superior to that of DGC and GWF techniques.

Research paper thumbnail of ISSN 2320-5407 International Journal of Advanced Research (2016), Volume 4, Issue 4, 1112-1115

Ula M. R. Al-Kawaz. Background:A variety of techniques have been developed to separate motile and... more Ula M. R. Al-Kawaz. Background:A variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive techniques. Objectives:To compare the asthenozoospermic semen outcomes of three in vitro sperm activation (ISA) techniques and evaluate the efficacy, namely; Density gradient centrifugation (DGC) technique, Glass wool filtration technique and Max pure technique(combination of DGC and GWF techniques). Methods:Forty three infertile men with asthenozoospermia were involved in this study. Sperm parameters assessed according to WHO (2010 and 1999). Postactivation of each sample divided into three aliquots, the first one using DGC, the second using GWF, and last one using Max pure technique. Results:A significant increase (P<0.05) of sperm motility, progressive sperm motility and normal sperm morphology when using Max pure technique as compared to DGC and GWF techniques....

Research paper thumbnail of New Approach of Hyaluronic Acid Bound Spermatozoa-ICSI in Iraqi Infertile Patients

Indian Journal of Public Health Research & Development

Research paper thumbnail of The New Approach of Hyaluronic Binding Assay in Relevance to Sperm Activation by Direct Swim-up Technique in Iraqi Infertile Men

Iraqi Journal of Embryos and Infertility Researches

Hyaluronic acid (HA) is a polysaccharide, that composed in the extracellular matrix of the cumulu... more Hyaluronic acid (HA) is a polysaccharide, that composed in the extracellular matrix of the cumulus cells, and considered to take function in choosing mature spermatozoa. The Sperm-hyaluronic binding assay (HBA) is significant diagnostic instrument for suspected male infertility when examining the semen. The HBA slide supplies the ratio of mature binding sperm in the specimen. To evaluate sperm parameters, and to evaluate the (HBA Score percentage (%) before and after activation. Twenty-five infertile couples a sharer in this current study through their attendance to the Al Nahrain University. Males undergoing seminal fluid analysis were done according to (WHO 2010, and WHO 1999). Sperm parameters assessed and calculate the HBA Score% pre-and-post-direct swim-up technique, the results were statistically tested. In Post-activation there was considerable increment noticed in the progressive motility and morphology of spermatozoa, likewise significant improvement in the (HBA score %). Furthermore, considerable reduction in the concentration, agglutination of spermatozoa and round cells count, when compared to pre-activation. The mean of binding of spermatozoa to hyaluronan pre-activation (direct swim-up technique) was less than normal limit for normozoospermic males. However, it significantly improves post-activation.

Research paper thumbnail of A NEW SPERM PREPARATION TECHNIQUE BY COMBINATION OF DENSITY GRADIENT CENTRIFUGATION AND GLASS WOOL FILTRATION TECHNIQUES VERSUS EACH ONE ALONE FOR INFERTILE MEN WITH ASTHENOZOOSPERMIA

Background:-A variety of techniques have been developed to separate motile and morphologically no... more Background:-A variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive techniques. Objectives:-To compare the asthenozoospermic semen outcomes of three in vitro sperm activation (ISA) techniques and evaluate the efficacy, namely; Density gradient centrifugation (DGC) technique, Glass wool filtration technique and Max pure technique(combination of DGC and GWF techniques). Methods:-Forty three infertile men with asthenozoospermia were involved in this study. Sperm parameters assessed according to WHO (2010 and 1999). Post-activation of each sample divided into three aliquots, the first one using DGC, the second using GWF, and last one using Max pure technique. Results:-A significant increase (P<0.05) of sperm motility, progressive sperm motility and normal sperm morphology when using Max pure technique as compared to DGC and GWF techniques. Also, there was a significant increase (P<0.05) for the same parameters when using DGC technique as compared to the GWF technique. Conclusions:-Using Max pure technique for semen sample with decreased in the sperm motility were superior to that of DGC and GWF techniques. Copy Right, IJAR, 2016,. All rights reserved.