Fábio David Couto - Academia.edu (original) (raw)

Papers by Fábio David Couto

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2013

INTRODUCTION: Atherosclerosis and correlated cardiovascular problems, whose mechanical and physio... more INTRODUCTION: Atherosclerosis and correlated cardiovascular problems, whose mechanical and physiological disorders cause thickening and hardening of blood vessels, are among the main causes of death worldwide. OBJECTIVE: To assess plasma concentrations of biomarkers from the lipid metabolism and carotid doppler sonography results by correlating them with atherogenic carotid disease. METHODS: the study comprised 66 patients aged 57.5 ± 15.5 years (20-77), from which 63% were female. Serum markers and doppler sonography images were used to evaluate the association with atherogenic carotid disease (ACD). RESULTS: There was a higher prevalence of ACD among females (33% vs. 15%), age range 56-65, showing a relative risk (RR) of 1.56 among females (p < 0.002; Fisher, Katz). Concerning high density lipoprotein cholesterol (HDL-C) and carotid stenosis classification, North American Symptomatic Carotid Endarterectomy Trial (NASCET), 76% of patients showed HDL-C within the protection range...

Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high preva-lence wo... more Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high preva-lence worldwide. Brazil has a prevalence of 0.1 to 0.3 % of newborns with sickle cell anemia and 20.0 to 25.0 % of heterozygous α2 thalassemia among African Brazilians. In the present study, we investigated the presence of variant hemo-globins and α23.7 Kb and α24.2 Kb thalassemia in newborns from Salvador, Bahia, Brazil. Samples of umbilical cord blood from a total of 590 new-borns were analyzed, of which 57 (9.8%) were FAS; 36 (6.5%) FAC; one (0.2%) SF; and five (0.9%) FSC. One hundred fourteen (22.2%) new-borns had α23.7 Kb thalassemia, of whom 101 (19.7%) were heterozygous and 13 (2.5%) ho-mozygous, showing statistical significance for hematological data between newborns with normal α genes and α23.7 Kb thalassemia carri-ers. The α24.2 Kb thalassemia was not found. Fre-quencies found in the present study confirm that hemoglobinopathies are a public health problem in Brazil, emphasizing the n...

The C677T polymorphism in the methylenetet ra h y d rofolate reductase gene (MTHFR) is associated... more The C677T polymorphism in the methylenetet ra h y d rofolate reductase gene (MTHFR) is associated with an increase in total homocysteine serum levels (tHcy), described as a risk factor for c a rd i ovascular disease. Eight hundred fort y t h ree neonates from two different maternity h o sp i t a l s , one public and another priva t e , in Salva d o r, Ba h i a , Brazil were screened for this polymorphism by PCR and RFLP. The T-allele frequency in the total sample was 0.23, and the p re valence rates of heterozygous and homoz ygous carriers were 36.2% and 5.3%, re s p e c t i ve l y. The T-allele frequency differed and the T/T g e n otype was more pre valent at the private maternity hospital. The hemoglobin (Hb) profile was investigated by HPLC in 763 new b o r n s . The frequency of variant Hb was higher at the public than at the private maternity hospital. The association of the C677T polymorphism and the Hb profile was investigated in 683 new b o r n s , s h owing a re l a t i vel...

The homocysteine metabolism is affected by nutritional status of B12 and folate vitamins or can r... more The homocysteine metabolism is affected by nutritional status of B12 and folate vitamins or can results from genetics mutations of genes responsable for proteins expression involved in re-methylation or trans-sulphuration metabolism pathway. A common mutation, C677T, in the methylenetetrahydrofolate reductase gene (MTHFR) reduces the MTHFR activity and increase the total homocysteine serum levels (tHcy). Hyperhomocysteinaemia has been identified as a risk factor for cardiovascular disease as established for variant hemoglobins. Here we investigated the C677T mutation prevalence in MTHFR gene by PCR and RFLP techniques, in 843 neonates from Salvador-Bahia-Brazil, and its coexistence with variant hemoglobins, which was determinated by HPLC. We estimated homocysteine, B12, and folate vitamins serum levels concentrations by chemiluminescence in a subgroup of 75 neonates with hemoglobin AA pattern, carriers different genotypes for MTHFR polimorphism: 25 wide type (C/C), 25 heterozygous (...

Alexandria: Revista de Educação em Ciência e Tecnologia

Este estudo utilizou o método de análise de conteúdo para investigar a abordagem sobre a doença f... more Este estudo utilizou o método de análise de conteúdo para investigar a abordagem sobre a doença falciforme (DF) em livros didáticos (LDs) de Biologia do ensino médio publicados no Brasil. Os resultados mostraram a necessidade de reavaliar informações disponibilizadas pelos LDs para evitar estigmatização e a perpetuação de conceitos que interferem no patrimônio cultural dessas pessoas. Apesar da frequência elevada do alelo que codifica a hemoglobina S em todo o Brasil, dados sobre a epidemiologia desta mutação não foram encontrados nos livros. Além disso, conceitos sobre o autocuidado, a qualidade e a expectativa de vida das pessoas com DF precisam ser revisitados. Uma vez que os LDs constituem um dos meios de disseminação de informações mais utilizados pelos estudantes nas escolas públicas brasileiras, a atualização das informações fornecidas torna-se necessária à luz de novos conhecimentos que têm impactos no entendimento da DF e na percepção das pessoas a seu respeito, tanto aquel...

Journal of Biophysical Chemistry

End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The... more End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The study evaluates the effect of hemodialysis (HD) on HDL-remodeling between pre-and post-HD. Was conducted a cross-sectional study with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador, Brazil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3-4 hours per week, with a polysulfone low-flow basicdialyzing-membrane and unfractionated-heparin. HDL Phospholipid-incorporation was measured by 14 C-PL-scintillation-counting, expressed as % 14 C-PL/mL/hour. Paraoxonase (PON-1) activity was measured by spectrophotometry using paraoxon as substrate. Cardiovascular risk ratios and atherogenic index of plasma were calculated. Total cholesterol, HDL-C and non-HDL-C increased at post-HD on all age groups, but without triglycerides (TG) changes. TG/HDL-C decreased in 30-39 and 40-49 year (y) at post-HD (p < 0.05). LDL-C/apoB increased in >60 y, after HD (p < 0.05). HDL-C/apo-AI increased in 40-49 y (p < 0.05) and >60 (p < 0.01). On the other hand, non-HDL-C/HDL-C reduced in 40-49 and >60 y, at post-HD (p < 0.05). The linear-correlation between % 14 C-PL-incorporation and non-HDL-C/HDL-C

Revista Baiana De Saude Publica, Jul 5, 2013

O Programa de Triagem Neonatal (PTN) realizado pelo SUS na Bahia abrange o diagnóstico da fenilce... more O Programa de Triagem Neonatal (PTN) realizado pelo SUS na Bahia abrange o diagnóstico da fenilcetonúria (PKU), hipotireoidismo congênito (HC) e hemoglobinopatias, patologias que devem ser tratadas precocemente. O objetivo deste artigo é avaliar a cobertura do PTN e a incidência das doenças triadas em Cruz das Almas e Valença, Bahia. Trata-se de estudo descritivo de incidência das doenças triadas pelo Serviço de Referencia em Triagem Neonatal (SRTN)-APAE Salvador, com dados obtidos nos registros das Secretarias Municipais de Saúde (SMS) de Cruz das Almas e Valença, do Serviço de Referência em Tiragem Neonatal (SRTN) e do DATASUS, entre 2001 e 2009. Os resultados indicam que a cobertura observada foi crescente, alcançando 87,6% e 88,8%, respectivamente, em Valença e Cruz das Almas, em 2009. Nestes municípios, encontrou-se incidência de dois casos/1000 nascidos vivos (NV) para hemoglobinas variantes; 0,5 casos/1000NV para HC e nenhum caso de PKU, respectivamente. Incongruências quanto ao número de nativivos e amostras triadas pelo PTN foram observadas. Concluiu-se que houve aumento da cobertura do PTN, com incidência relevante de hemoglobinopatias nos municípios estudados. O sucesso da cobertura do programa depende da adesão dos municípios, sendo necessária a implantação de um serviço de gestão dessas informações nas SMSs para planejamento de políticas de saúde pública.

Journal of Biophysical Chemistry, Apr 23, 2014

Dyslipidemia may influence enzymes and transfer proteins needed to the lipoprotein particle remod... more Dyslipidemia may influence enzymes and transfer proteins needed to the lipoprotein particle remodeling. Calculated indices and evaluation of lipoprotein particle size have widely been used to predict cardiovascular risk. The aim of this study was to evaluate HDL particle size and LDL particle size estimate based on TG/HDL-C as well as apoB/apoA-I ratio as possible marker and atherogenic indices, respectively, of cardiovascular disease risk in the presence of dyslipidemia. We evaluated 100 individuals of both gender, without treatment with lipid-lowering drugs, 27 normolipidemic and 73 dyslipidemic, such as isolated hypercholesterolemia (n = 16), isolated A. P. C. Santos et al. 25 hypertriglyceridemia (n = 17), low HDL-C (n = 26) and mixed dyslipidemia (n = 14). The HDL particle size did not differ between groups. The TG/HDL-C ratio was higher in groups with isolated hypertriglyceridemia (4.2 ± 1.5), low HDL-C (5.2 ± 3.1) and mixed dyslipidemia (5.3 ± 1.6). The apoB/apoA-I ratio was increased in all groups of dyslipidemia (apoB/apoA-I > 0.5) when compared to normolipidemic (apoB/apoA-I = 0.5, p < 0.001). There was a positive linear correlation between the TG/HDL-C ratio and the apoB/apoA-I ratio in low HDL-C group (r = 0.507, p = 0.008, Spearman). The results suggest that the evaluations of lipoproteins particles remodeling markers and the use of calculated indices may contribute to the evaluation of cardiovascular disease risk when dyslipidemia take place.

Journal of Medical Microbiology

Introduction. Sickle cell disease (SCD) children have a high susceptibility to pneumococcal infec... more Introduction. Sickle cell disease (SCD) children have a high susceptibility to pneumococcal infection. For this reason, they are routinely immunized with pneumococcal vaccines and use antibiotic prophylaxis (AP). Hypothesis/Gap Statement. Yet, little is known about SCD children’s gut microbiota. If antibiotic-resistant Enterobacterales may colonize people on AP, we hypothesized that SCD children on AP are colonized by resistant enterobacteria species. Objective. To evaluate the effect of continuous AP on Enterobacterales gut colonization from children with SCD. Methodology. We analysed 30 faecal swabs from SCD children on AP and 21 swabs from children without the same condition. Enterobacterales was isolated on MacConkey agar plates and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (bioMérieux, Marcy l'Etoile, France). We performed the antibiogram by Vitek 2 system (bioMérieux, Marcy l'Etoile, France), and the resis...

Revista Baiana de Saúde Pública

Frente a lacuna formativa dos profissionais da atenção básica e da educação para o manejo e acomp... more Frente a lacuna formativa dos profissionais da atenção básica e da educação para o manejo e acompanhamento adequados das pessoas que vivem com a Doença Falciforme, considerada a doença genética de maior prevalência no Brasil, este trabalho teve como objetivo capacitar profissionais das áreas da saúde e da educação em nove municípios da região do Recôncavo do estado da Bahia, entre 2014 e 2015. As capacitações ocorreram com equipe multidisciplinar, envolvendo enfermeiros, médicos, dentistas, discentes e docentes da Universidade Federal do Recôncavo da Bahia, membros da Secretaria de Saúde do Estado da Bahia, Secretarias Municipais de Saúde, Núcleo Regional de Saúde Leste e apoio financeiro da Fundação de Amparo à Pesquisa do Estado da Bahia. Os resultados indicaram a participação de 702 profissionais no programa: 29 (4,1%) médicos, 101 (14,4%) enfermeiros, 43 (6,1%) dentistas, 69 (9,8%) outros profissionais graduados (psicólogos, fisioterapeutas, nutricionistas, professores) e 460 (6...

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2012

Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Pu... more Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Publicado em 20/10/12

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2011

Primeira submissão em 14/01/10 Última submissão em 24/02/11 Aceito para publicação em 28/02/11 Pu... more Primeira submissão em 14/01/10 Última submissão em 24/02/11 Aceito para publicação em 28/02/11 Publicado em 20/04/11 Razão triglicérides/HDL-C e proteína C reativa de alta sensibilidade na avaliação do risco cardiovascular Triglycerides/HDL-C ratio and high sensible C-reactive protein to the evaluation of cardiovascular risk

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2013

Cardiovascular problems, from which atherosclerosis complications are worth noting, are among the... more Cardiovascular problems, from which atherosclerosis complications are worth noting, are among the main causes of death worldwide. It is characterized by impaired mechanical and physiological conditions that cause thickening and hardening of coronary, cerebral and peripheral vessels, leading to chronic

Jornal Brasileiro De Patologia E Medicina Laboratorial, Oct 1, 2012

Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Pu... more Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Publicado em 20/10/12

Neuroscience Letters

The methylenetetrahydrofolate reductase (MTHFR) gene has been recently considered as a candidate ... more The methylenetetrahydrofolate reductase (MTHFR) gene has been recently considered as a candidate gene for Alzheimer's disease (AD). MTHFR is a key enzyme in the metabolism of homocysteine and elevated levels of that amino acid have been associated to Vascular Dementia and AD. A T-->C transition at codon 677 produces a thermolabile type of the enzyme. However, contrasting results on the distribution of the MTHFR C677T common polymorphism in AD have been published. We analyzed the distribution of the MTHFR and apolipoprotein E (APOE) polymorphisms in Italian patients with sporadic AD. The distribution of the C677T polymorphism did not differ in AD and controls. Our data suggest that the MTHFR polymorphism does not contribute to genetic susceptibility in Italian sporadic AD and does not mitigate the effect of ApoE epsilon4 allele on AD risk.

Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high prevalence wor... more Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high prevalence worldwide. Brazil has a prevalence of 0.1 to 0.3% of newborns with sickle cell anemia and 20.0 to 25.0% of heterozygous α 2 thalassemia among African Brazilians. In the present study, we investigated the presence of variant hemoglobins and α 2 3.7 Kb and α 2 4.2 Kb thalassemia in newborns from Salvador, Bahia, Brazil. Samples of umbilical cord blood from a total of 590 newborns were analyzed, of which 57 (9.8%) were FAS; 36 (6.5%) FAC; one (0.2%) SF; and five (0.9%) FSC. One hundred fourteen (22.2%) newborns had α 2 3.7 Kb thalassemia, of whom 101 (19.7%) were heterozygous and 13 (2.5%) homozygous, showing statistical significance for hematological data between newborns with normal α genes and α 2 3.7 Kb thalassemia carriers. The α 2 4.2 Kb thalassemia was not found. Frequencies found in the present study confirm that hemoglobinopathies are a public health problem in Brazil, emphasizing the need for neonatal screening and genetic counseling programs.

Cadernos de Saúde Pública

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2013

INTRODUCTION: Atherosclerosis and correlated cardiovascular problems, whose mechanical and physio... more INTRODUCTION: Atherosclerosis and correlated cardiovascular problems, whose mechanical and physiological disorders cause thickening and hardening of blood vessels, are among the main causes of death worldwide. OBJECTIVE: To assess plasma concentrations of biomarkers from the lipid metabolism and carotid doppler sonography results by correlating them with atherogenic carotid disease. METHODS: the study comprised 66 patients aged 57.5 ± 15.5 years (20-77), from which 63% were female. Serum markers and doppler sonography images were used to evaluate the association with atherogenic carotid disease (ACD). RESULTS: There was a higher prevalence of ACD among females (33% vs. 15%), age range 56-65, showing a relative risk (RR) of 1.56 among females (p < 0.002; Fisher, Katz). Concerning high density lipoprotein cholesterol (HDL-C) and carotid stenosis classification, North American Symptomatic Carotid Endarterectomy Trial (NASCET), 76% of patients showed HDL-C within the protection range...

Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high preva-lence wo... more Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high preva-lence worldwide. Brazil has a prevalence of 0.1 to 0.3 % of newborns with sickle cell anemia and 20.0 to 25.0 % of heterozygous α2 thalassemia among African Brazilians. In the present study, we investigated the presence of variant hemo-globins and α23.7 Kb and α24.2 Kb thalassemia in newborns from Salvador, Bahia, Brazil. Samples of umbilical cord blood from a total of 590 new-borns were analyzed, of which 57 (9.8%) were FAS; 36 (6.5%) FAC; one (0.2%) SF; and five (0.9%) FSC. One hundred fourteen (22.2%) new-borns had α23.7 Kb thalassemia, of whom 101 (19.7%) were heterozygous and 13 (2.5%) ho-mozygous, showing statistical significance for hematological data between newborns with normal α genes and α23.7 Kb thalassemia carri-ers. The α24.2 Kb thalassemia was not found. Fre-quencies found in the present study confirm that hemoglobinopathies are a public health problem in Brazil, emphasizing the n...

The C677T polymorphism in the methylenetet ra h y d rofolate reductase gene (MTHFR) is associated... more The C677T polymorphism in the methylenetet ra h y d rofolate reductase gene (MTHFR) is associated with an increase in total homocysteine serum levels (tHcy), described as a risk factor for c a rd i ovascular disease. Eight hundred fort y t h ree neonates from two different maternity h o sp i t a l s , one public and another priva t e , in Salva d o r, Ba h i a , Brazil were screened for this polymorphism by PCR and RFLP. The T-allele frequency in the total sample was 0.23, and the p re valence rates of heterozygous and homoz ygous carriers were 36.2% and 5.3%, re s p e c t i ve l y. The T-allele frequency differed and the T/T g e n otype was more pre valent at the private maternity hospital. The hemoglobin (Hb) profile was investigated by HPLC in 763 new b o r n s . The frequency of variant Hb was higher at the public than at the private maternity hospital. The association of the C677T polymorphism and the Hb profile was investigated in 683 new b o r n s , s h owing a re l a t i vel...

The homocysteine metabolism is affected by nutritional status of B12 and folate vitamins or can r... more The homocysteine metabolism is affected by nutritional status of B12 and folate vitamins or can results from genetics mutations of genes responsable for proteins expression involved in re-methylation or trans-sulphuration metabolism pathway. A common mutation, C677T, in the methylenetetrahydrofolate reductase gene (MTHFR) reduces the MTHFR activity and increase the total homocysteine serum levels (tHcy). Hyperhomocysteinaemia has been identified as a risk factor for cardiovascular disease as established for variant hemoglobins. Here we investigated the C677T mutation prevalence in MTHFR gene by PCR and RFLP techniques, in 843 neonates from Salvador-Bahia-Brazil, and its coexistence with variant hemoglobins, which was determinated by HPLC. We estimated homocysteine, B12, and folate vitamins serum levels concentrations by chemiluminescence in a subgroup of 75 neonates with hemoglobin AA pattern, carriers different genotypes for MTHFR polimorphism: 25 wide type (C/C), 25 heterozygous (...

Alexandria: Revista de Educação em Ciência e Tecnologia

Este estudo utilizou o método de análise de conteúdo para investigar a abordagem sobre a doença f... more Este estudo utilizou o método de análise de conteúdo para investigar a abordagem sobre a doença falciforme (DF) em livros didáticos (LDs) de Biologia do ensino médio publicados no Brasil. Os resultados mostraram a necessidade de reavaliar informações disponibilizadas pelos LDs para evitar estigmatização e a perpetuação de conceitos que interferem no patrimônio cultural dessas pessoas. Apesar da frequência elevada do alelo que codifica a hemoglobina S em todo o Brasil, dados sobre a epidemiologia desta mutação não foram encontrados nos livros. Além disso, conceitos sobre o autocuidado, a qualidade e a expectativa de vida das pessoas com DF precisam ser revisitados. Uma vez que os LDs constituem um dos meios de disseminação de informações mais utilizados pelos estudantes nas escolas públicas brasileiras, a atualização das informações fornecidas torna-se necessária à luz de novos conhecimentos que têm impactos no entendimento da DF e na percepção das pessoas a seu respeito, tanto aquel...

Journal of Biophysical Chemistry

End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The... more End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The study evaluates the effect of hemodialysis (HD) on HDL-remodeling between pre-and post-HD. Was conducted a cross-sectional study with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador, Brazil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3-4 hours per week, with a polysulfone low-flow basicdialyzing-membrane and unfractionated-heparin. HDL Phospholipid-incorporation was measured by 14 C-PL-scintillation-counting, expressed as % 14 C-PL/mL/hour. Paraoxonase (PON-1) activity was measured by spectrophotometry using paraoxon as substrate. Cardiovascular risk ratios and atherogenic index of plasma were calculated. Total cholesterol, HDL-C and non-HDL-C increased at post-HD on all age groups, but without triglycerides (TG) changes. TG/HDL-C decreased in 30-39 and 40-49 year (y) at post-HD (p < 0.05). LDL-C/apoB increased in >60 y, after HD (p < 0.05). HDL-C/apo-AI increased in 40-49 y (p < 0.05) and >60 (p < 0.01). On the other hand, non-HDL-C/HDL-C reduced in 40-49 and >60 y, at post-HD (p < 0.05). The linear-correlation between % 14 C-PL-incorporation and non-HDL-C/HDL-C

Revista Baiana De Saude Publica, Jul 5, 2013

O Programa de Triagem Neonatal (PTN) realizado pelo SUS na Bahia abrange o diagnóstico da fenilce... more O Programa de Triagem Neonatal (PTN) realizado pelo SUS na Bahia abrange o diagnóstico da fenilcetonúria (PKU), hipotireoidismo congênito (HC) e hemoglobinopatias, patologias que devem ser tratadas precocemente. O objetivo deste artigo é avaliar a cobertura do PTN e a incidência das doenças triadas em Cruz das Almas e Valença, Bahia. Trata-se de estudo descritivo de incidência das doenças triadas pelo Serviço de Referencia em Triagem Neonatal (SRTN)-APAE Salvador, com dados obtidos nos registros das Secretarias Municipais de Saúde (SMS) de Cruz das Almas e Valença, do Serviço de Referência em Tiragem Neonatal (SRTN) e do DATASUS, entre 2001 e 2009. Os resultados indicam que a cobertura observada foi crescente, alcançando 87,6% e 88,8%, respectivamente, em Valença e Cruz das Almas, em 2009. Nestes municípios, encontrou-se incidência de dois casos/1000 nascidos vivos (NV) para hemoglobinas variantes; 0,5 casos/1000NV para HC e nenhum caso de PKU, respectivamente. Incongruências quanto ao número de nativivos e amostras triadas pelo PTN foram observadas. Concluiu-se que houve aumento da cobertura do PTN, com incidência relevante de hemoglobinopatias nos municípios estudados. O sucesso da cobertura do programa depende da adesão dos municípios, sendo necessária a implantação de um serviço de gestão dessas informações nas SMSs para planejamento de políticas de saúde pública.

Journal of Biophysical Chemistry, Apr 23, 2014

Dyslipidemia may influence enzymes and transfer proteins needed to the lipoprotein particle remod... more Dyslipidemia may influence enzymes and transfer proteins needed to the lipoprotein particle remodeling. Calculated indices and evaluation of lipoprotein particle size have widely been used to predict cardiovascular risk. The aim of this study was to evaluate HDL particle size and LDL particle size estimate based on TG/HDL-C as well as apoB/apoA-I ratio as possible marker and atherogenic indices, respectively, of cardiovascular disease risk in the presence of dyslipidemia. We evaluated 100 individuals of both gender, without treatment with lipid-lowering drugs, 27 normolipidemic and 73 dyslipidemic, such as isolated hypercholesterolemia (n = 16), isolated A. P. C. Santos et al. 25 hypertriglyceridemia (n = 17), low HDL-C (n = 26) and mixed dyslipidemia (n = 14). The HDL particle size did not differ between groups. The TG/HDL-C ratio was higher in groups with isolated hypertriglyceridemia (4.2 ± 1.5), low HDL-C (5.2 ± 3.1) and mixed dyslipidemia (5.3 ± 1.6). The apoB/apoA-I ratio was increased in all groups of dyslipidemia (apoB/apoA-I > 0.5) when compared to normolipidemic (apoB/apoA-I = 0.5, p < 0.001). There was a positive linear correlation between the TG/HDL-C ratio and the apoB/apoA-I ratio in low HDL-C group (r = 0.507, p = 0.008, Spearman). The results suggest that the evaluations of lipoproteins particles remodeling markers and the use of calculated indices may contribute to the evaluation of cardiovascular disease risk when dyslipidemia take place.

Journal of Medical Microbiology

Introduction. Sickle cell disease (SCD) children have a high susceptibility to pneumococcal infec... more Introduction. Sickle cell disease (SCD) children have a high susceptibility to pneumococcal infection. For this reason, they are routinely immunized with pneumococcal vaccines and use antibiotic prophylaxis (AP). Hypothesis/Gap Statement. Yet, little is known about SCD children’s gut microbiota. If antibiotic-resistant Enterobacterales may colonize people on AP, we hypothesized that SCD children on AP are colonized by resistant enterobacteria species. Objective. To evaluate the effect of continuous AP on Enterobacterales gut colonization from children with SCD. Methodology. We analysed 30 faecal swabs from SCD children on AP and 21 swabs from children without the same condition. Enterobacterales was isolated on MacConkey agar plates and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (bioMérieux, Marcy l'Etoile, France). We performed the antibiogram by Vitek 2 system (bioMérieux, Marcy l'Etoile, France), and the resis...

Revista Baiana de Saúde Pública

Frente a lacuna formativa dos profissionais da atenção básica e da educação para o manejo e acomp... more Frente a lacuna formativa dos profissionais da atenção básica e da educação para o manejo e acompanhamento adequados das pessoas que vivem com a Doença Falciforme, considerada a doença genética de maior prevalência no Brasil, este trabalho teve como objetivo capacitar profissionais das áreas da saúde e da educação em nove municípios da região do Recôncavo do estado da Bahia, entre 2014 e 2015. As capacitações ocorreram com equipe multidisciplinar, envolvendo enfermeiros, médicos, dentistas, discentes e docentes da Universidade Federal do Recôncavo da Bahia, membros da Secretaria de Saúde do Estado da Bahia, Secretarias Municipais de Saúde, Núcleo Regional de Saúde Leste e apoio financeiro da Fundação de Amparo à Pesquisa do Estado da Bahia. Os resultados indicaram a participação de 702 profissionais no programa: 29 (4,1%) médicos, 101 (14,4%) enfermeiros, 43 (6,1%) dentistas, 69 (9,8%) outros profissionais graduados (psicólogos, fisioterapeutas, nutricionistas, professores) e 460 (6...

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2012

Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Pu... more Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Publicado em 20/10/12

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2011

Primeira submissão em 14/01/10 Última submissão em 24/02/11 Aceito para publicação em 28/02/11 Pu... more Primeira submissão em 14/01/10 Última submissão em 24/02/11 Aceito para publicação em 28/02/11 Publicado em 20/04/11 Razão triglicérides/HDL-C e proteína C reativa de alta sensibilidade na avaliação do risco cardiovascular Triglycerides/HDL-C ratio and high sensible C-reactive protein to the evaluation of cardiovascular risk

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2013

Cardiovascular problems, from which atherosclerosis complications are worth noting, are among the... more Cardiovascular problems, from which atherosclerosis complications are worth noting, are among the main causes of death worldwide. It is characterized by impaired mechanical and physiological conditions that cause thickening and hardening of coronary, cerebral and peripheral vessels, leading to chronic

Jornal Brasileiro De Patologia E Medicina Laboratorial, Oct 1, 2012

Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Pu... more Primeira submissão em 14/01/12 Última submissão em 30/01/12 Aceito para publicação em 09/05/12 Publicado em 20/10/12

Neuroscience Letters

The methylenetetrahydrofolate reductase (MTHFR) gene has been recently considered as a candidate ... more The methylenetetrahydrofolate reductase (MTHFR) gene has been recently considered as a candidate gene for Alzheimer's disease (AD). MTHFR is a key enzyme in the metabolism of homocysteine and elevated levels of that amino acid have been associated to Vascular Dementia and AD. A T-->C transition at codon 677 produces a thermolabile type of the enzyme. However, contrasting results on the distribution of the MTHFR C677T common polymorphism in AD have been published. We analyzed the distribution of the MTHFR and apolipoprotein E (APOE) polymorphisms in Italian patients with sporadic AD. The distribution of the C677T polymorphism did not differ in AD and controls. Our data suggest that the MTHFR polymorphism does not contribute to genetic susceptibility in Italian sporadic AD and does not mitigate the effect of ApoE epsilon4 allele on AD risk.

Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high prevalence wor... more Hemoglobinopathies are hereditary disorders of the hemoglobin molecule with a high prevalence worldwide. Brazil has a prevalence of 0.1 to 0.3% of newborns with sickle cell anemia and 20.0 to 25.0% of heterozygous α 2 thalassemia among African Brazilians. In the present study, we investigated the presence of variant hemoglobins and α 2 3.7 Kb and α 2 4.2 Kb thalassemia in newborns from Salvador, Bahia, Brazil. Samples of umbilical cord blood from a total of 590 newborns were analyzed, of which 57 (9.8%) were FAS; 36 (6.5%) FAC; one (0.2%) SF; and five (0.9%) FSC. One hundred fourteen (22.2%) newborns had α 2 3.7 Kb thalassemia, of whom 101 (19.7%) were heterozygous and 13 (2.5%) homozygous, showing statistical significance for hematological data between newborns with normal α genes and α 2 3.7 Kb thalassemia carriers. The α 2 4.2 Kb thalassemia was not found. Frequencies found in the present study confirm that hemoglobinopathies are a public health problem in Brazil, emphasizing the need for neonatal screening and genetic counseling programs.

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