FABIO ALESSANDRO PIERI - Academia.edu (original) (raw)

Papers by FABIO ALESSANDRO PIERI

BMC Veterinary Research, 2016

Background: Periodontal disease is a highly prevalent illness that affects many dogs, reaching up... more Background: Periodontal disease is a highly prevalent illness that affects many dogs, reaching up to 85 % prevalence in individuals over the age of 4 years. Currently the drug of choice for combating the formation of dental plaque in these animals, the etiologic agent of the disease, is chlorhexidine, which has several side effects reported. Thus, surveys are conducted throughout the world in order to identify potential substitutes for antimicrobial therapy and prevention of periodontal disease. The objective of the work was to evaluate the antimicrobial activity of β-caryophyllene against bacteria from dog's dental plaque in vitro and in vivo. The minimum inhibitory concentration was evaluated by agar microdilution assay, the induction or inhibition of bacterial adherence by sub-inhibitory concentrations in 96-well plates, and reduction of dental plaque formation in mongrel dogs subjected to topical solution with β-caryophyllene for 15 days. Results: Results showed minimum inhibitory concentrations above 100 mg/mL for 25 % of the isolates, 100 mg/mL for 3 %, 50 mg/mL for 25 %, 25 mg/mL for 12 %, 12.5 mg/mL for 19 % and 6.25 mg/mL for 16 %. Bacterial adherences of three Enterococcus sp., one Streptococcus sp., one Haemophilus sp., one Aerococcus sp., one Bacillus sp. and one Lactococcus sp. isolates were inhibited by subinhibitory concentration. One Lactococcus sp., one Bacillus sp. and one Streptococcus sp. were stimulated to adhere by concentrations of 0.19, 1.56 and 0.78 mg/mL, respectively. In vivo assay showed reduction in dental plaque formation by β-caryophyllene, with final plaque coverage of 23.3 ± 2.6 % of the total area of the teeth, with significant difference compared with chlorhexidine group (37.5 ± 3.7 %-p < 0.05) and negative control group (65.5 ± 2.5 %-p < 0.001). Conclusions: The results showed that β-caryophyllene has antimicrobial activity against the proliferation of dog's dental plaque-forming bacteria representing a suitable alternative to the use of chlorhexidine in prophylaxis and treatment of periodontal disease of dogs.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2012

Antimicrobial profile screening of two oils of Copaifera genus [Avaliação do perfil antimicrobian... more Antimicrobial profile screening of two oils of Copaifera genus [Avaliação do perfil antimicrobiano de dois óleos do gênero Copaifera]

Arquivos do Mudi

O ensino público brasileiro enfrenta atualmente inúmeros problemas, desde a falta de infraestrutu... more O ensino público brasileiro enfrenta atualmente inúmeros problemas, desde a falta de infraestrutura das escolas até índices inadequados de Alfabetização Científica (AC) dos estudantes. Portanto, ensinar os conteúdos relacionados a Biologia, principalmente a Microbiologia de maneira contextualizada tem sido desafiador. Na expectativa de contribuir para o ensino de Microbiologia no ensino médio público, o trabalho visou apresentar novas propostas de ensino através do E-book: “A Biologia que a gente não vê: aulas práticas para escolas sem laboratórios”. A obra foi elaborada após a realização de levantamentos bibliográficos sobre tipos de metodologias ativas e práticas experimentais relacionadas a Microbiologia. A metodologia Ensino por Investigação foi selecionada pela capacidade de proporcionar o desenvolvimento de atividades de aprendizagem ativas para a promoção da AC e do pensamento crítico dos alunos, por meio da resolução de problemas contextualizados ao cotidiano, além da viabil...

Frontiers in microbiology, 2018

Mastitis is an inflammation of the mammary gland that causes major losses in the dairy industry. ... more Mastitis is an inflammation of the mammary gland that causes major losses in the dairy industry. spp. are among the main agents of this disease. Increased resistance to antibiotics is one of the causes of therapeutic failure. Plants, due to their broad chemodiversity, are an interesting source of new molecules with antibacterial activity. Using these compounds along with traditional antibiotics is a possible method for reversing resistance. The objective of this work was to determine the interactions between the activities of guttiferone-A and 7-epiclusianone, two active substances isolated from the fruits of , and traditional antibiotics against spp. isolated from bovine mastitis and known to be resistant to them. First, the MIC for the antibiotics and bioactive compounds was determined, followed by their activities, alone and in combination. Then, their cytotoxicity was measured in bovine mammary epithelial cells. Finally, molecular docking simulations were performed to elucidate ...

Foodborne pathogens and disease, Jan 29, 2016

The aim of this study was to characterize and compare Staphylococcus spp. isolated from hospitali... more The aim of this study was to characterize and compare Staphylococcus spp. isolated from hospitalized patients and beef marketed in the city of Porto Velho-RO, Brazil. The isolates were subjected to antibiogram tests, adherence capacity tests, detection of the mecA gene, and epidemiological investigation by the random amplified polymorphic DNA (RAPD) technique, using the primers M13 and H12. Among the 123 Staphylococcus spp. isolates, 50 were identified as S. aureus and 73 as coagulase-negative Staphylococcus; among the latter, 7 species were identified. It was observed that the coagulase-negative Staphylococcus isolates showed greater adhesion ability than S. aureus. The profile of antimicrobial susceptibility was different among isolates, all of which were susceptible to vancomycin and linezolid, and had high penicillin resistance rates, varying according to the bacterial class and the source. In this study, all strains were negative for mecA gene detection; however, 36% of S. aure...

Semina: Ciências Agrárias, 2011

O objetivo deste estudo foi avaliar o efeito antimicrobiano de dois diferentes óleos de copaíba s... more O objetivo deste estudo foi avaliar o efeito antimicrobiano de dois diferentes óleos de copaíba sobre isolados de Escherichia coli obtidos de leite mastítico. O ensaio antimicrobiano foi realizado utilizando o teste de difusão em agar, testando três soluções: um controle negativo e duas soluções dos diferentes óleos de copaíba. O teste foi realizado com 27 isolados de E. coli. Oito isolados (29.63%) foram inibidos pela solução com óleo de C. langsdorffii e sete pela solução com óleo de C. officinalis. Os resultados do presente estudo sugerem o óleo de copaiba como uma potencial fonte de novos e seletivos agentes para o tratamento da mastite, após posteriores testes em modelos in vivo.

BioMed Research International, 2014

Shigellosis is a global human health problem and the incidence is highest among children. In the ... more Shigellosis is a global human health problem and the incidence is highest among children. In the present work, mainShigellavirulence genes was examined by PCR and compared to symptoms of pediatric shigellosis. ThirtyShigellaisolates were identified from an etiologic study at which 1,339 children ranging 0–10 years old were enrolled.S. flexneriwas the most frequent species reaching 60.0% of isolates, 22.2% wereS. sonnei, and 6.6% were bothS. dysenteriaeandS. boydii. AllShigellainfected children had diarrhea, but not all were accompanied by others symptoms of bacillary dysentery. Among major virulence genes, the PCR typing revealedipaBCDwas present in all isolates, followed byIpaH7.8,set-1A,set-1B, sen/ospD3, virF,andinvE. The pathogenic potential of the ShET-1B subunit was observed in relation to dehydration (P<0.001) and ShET-2 related to the intestinal injury (P=0.033) evidenced by the presence of bloody diarrhea. Our results show associations among symptoms of shigellosis and v...

BioMed Research International, 2014

This study aims to assess and compare copaiba oleoresin ofCopaifera multijugaand 0.5% silver nitr... more This study aims to assess and compare copaiba oleoresin ofCopaifera multijugaand 0.5% silver nitrate for the induction of pleurodesis in an experimental model. Ninety-six male Wistar rats were divided into three groups: control (0.9% saline solution), copaiba (copaiba oil), and silver nitrate (0.5% silver nitrate). The substances were injected into the right pleural cavity and the alterations were observed macroscopically and microscopically at 24, 48, 72, and 504 h. The value of macroscopic alterations grade and acute inflammatory reaction grade means was higher in the 24 h copaiba group in relation to silver nitrate. Fibrosis and neovascularization means in the visceral pleura were higher in 504 h copaiba group in relation to the silver nitrate group. The grade of the alveolar edema mean was higher in the silver nitrate group in relation to the copaiba group, in which this alteration was not observed. The presence of bronchopneumonia was higher in the 24 h silver nitrate group (n ...

Foodborne Pathogens and Disease, 2014

This study aimed to assess raw milk consumption habits in the urban population of Viçosa, Minas G... more This study aimed to assess raw milk consumption habits in the urban population of Viçosa, Minas Gerais, Brazil, and the microbiological safety and quality of the fluid milk available in retail sales outlets in the same region. A simplified questionnaire regarding raw milk consumption was applied to the persons responsible for food acquisition in 411 residences. The regular consumption of raw milk was observed by 18.5% of the interviewers, and lack of knowledge of possible risks related to this food product. Microbiological safety and quality were assessed for raw (n = 69), pasteurized (n = 80), and ultra-high-temperature (UHT)-treated milk (n = 80) by analyzing the counts of mesophilic aerobes, coliforms, and Escherichia coli, and detection of Listeria monocytogenes and Salmonella spp.; raw milk samples were also subjected to enumeration of coagulasepositive Staphylococcus. Concerning raw milk, 59.4% of the samples were considered as produced in inadequate hygienic conditions, 5.8% of the samples presented counts of coagulase-positive Staphylococcus lower than 100 colony-forming units (CFU)/mL, and no samples presented with positive results for L. monocytogenes or Salmonella spp. All pasteurized and UHT milk samples presented with low counts of mesophilic aerobes and coliforms, while L. monocytogenes and Salmonella spp. were absent. The data demonstrated that raw milk was consumed by the population studied. Despite the absence of potential hazards, raw milk was of poor hygienic quality, in contrast with the processed fluid milk available in retail sales outlets that was safe and of good hygienic quality, highlighting the suitability of pasteurized and UHT milk for human consumption.

Acta Amazonica, 2014

Honeys are described possessing different properties including antimicrobial. Many studies have p... more Honeys are described possessing different properties including antimicrobial. Many studies have presented this activity of honeys produced by Apis mellifera bees, however studies including activities of stingless bees honeys are scarce. The aim of this study was to compare the antimicrobial activity of honeys collected in the Amazonas State from Melipona compressipes, Melipona seminigra and Apis mellifera against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Chromobacterium violaceum, and Candida albicans. Minimum inhibitory concentrations were determined using the agar dilution method with Müller-Hinton agar (for bacteria) or Saboraud agar (for yeast). Staphylococcus aureus and E. faecalis were inhibited by all honeys at concentrations below 12%, while E. coli and C. violaceum were inhibited by stingless bee honeys at concentrations between 10 and 20%. A. mellifera honey inhibited E. coli at a concentration of 7% and Candida violaceum at 0.7%. C. albicans were inh...

Natural Resources Management and Biological Sciences, 2020

Currently, the number of diseases has been increasing and reaching the population directly, and t... more Currently, the number of diseases has been increasing and reaching the population directly, and the deliberate use of drugs is creating resistance of pathogens in several drugs, a fact evidenced by the increased ineffectiveness of drugs and the persistence of infections in the body. Given this, it is necessary to search for new alternative drugs that can effectively promote effective therapy. It is possible to highlight, in Brazil, the diversity of the Amazonian flora, which has several species with considerable potential as a source of new molecules with identified biological activity. Thus, a literature review was conducted in order to describe the applications of some Amazonian extracts and their chemical characteristics and biological activity. The Amazon rain forest has considerable diversity of plant species with biological properties that may be useful to public health. Further research is needed to identify new compounds with health benefits.

BMC Veterinary Research, 2016

Background: Periodontal disease is a highly prevalent illness that affects many dogs, reaching up... more Background: Periodontal disease is a highly prevalent illness that affects many dogs, reaching up to 85 % prevalence in individuals over the age of 4 years. Currently the drug of choice for combating the formation of dental plaque in these animals, the etiologic agent of the disease, is chlorhexidine, which has several side effects reported. Thus, surveys are conducted throughout the world in order to identify potential substitutes for antimicrobial therapy and prevention of periodontal disease. The objective of the work was to evaluate the antimicrobial activity of β-caryophyllene against bacteria from dog's dental plaque in vitro and in vivo. The minimum inhibitory concentration was evaluated by agar microdilution assay, the induction or inhibition of bacterial adherence by sub-inhibitory concentrations in 96-well plates, and reduction of dental plaque formation in mongrel dogs subjected to topical solution with β-caryophyllene for 15 days. Results: Results showed minimum inhibitory concentrations above 100 mg/mL for 25 % of the isolates, 100 mg/mL for 3 %, 50 mg/mL for 25 %, 25 mg/mL for 12 %, 12.5 mg/mL for 19 % and 6.25 mg/mL for 16 %. Bacterial adherences of three Enterococcus sp., one Streptococcus sp., one Haemophilus sp., one Aerococcus sp., one Bacillus sp. and one Lactococcus sp. isolates were inhibited by subinhibitory concentration. One Lactococcus sp., one Bacillus sp. and one Streptococcus sp. were stimulated to adhere by concentrations of 0.19, 1.56 and 0.78 mg/mL, respectively. In vivo assay showed reduction in dental plaque formation by β-caryophyllene, with final plaque coverage of 23.3 ± 2.6 % of the total area of the teeth, with significant difference compared with chlorhexidine group (37.5 ± 3.7 %-p < 0.05) and negative control group (65.5 ± 2.5 %-p < 0.001). Conclusions: The results showed that β-caryophyllene has antimicrobial activity against the proliferation of dog's dental plaque-forming bacteria representing a suitable alternative to the use of chlorhexidine in prophylaxis and treatment of periodontal disease of dogs.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2012

Antimicrobial profile screening of two oils of Copaifera genus [Avaliação do perfil antimicrobian... more Antimicrobial profile screening of two oils of Copaifera genus [Avaliação do perfil antimicrobiano de dois óleos do gênero Copaifera]

Arquivos do Mudi

O ensino público brasileiro enfrenta atualmente inúmeros problemas, desde a falta de infraestrutu... more O ensino público brasileiro enfrenta atualmente inúmeros problemas, desde a falta de infraestrutura das escolas até índices inadequados de Alfabetização Científica (AC) dos estudantes. Portanto, ensinar os conteúdos relacionados a Biologia, principalmente a Microbiologia de maneira contextualizada tem sido desafiador. Na expectativa de contribuir para o ensino de Microbiologia no ensino médio público, o trabalho visou apresentar novas propostas de ensino através do E-book: “A Biologia que a gente não vê: aulas práticas para escolas sem laboratórios”. A obra foi elaborada após a realização de levantamentos bibliográficos sobre tipos de metodologias ativas e práticas experimentais relacionadas a Microbiologia. A metodologia Ensino por Investigação foi selecionada pela capacidade de proporcionar o desenvolvimento de atividades de aprendizagem ativas para a promoção da AC e do pensamento crítico dos alunos, por meio da resolução de problemas contextualizados ao cotidiano, além da viabil...

Frontiers in microbiology, 2018

Mastitis is an inflammation of the mammary gland that causes major losses in the dairy industry. ... more Mastitis is an inflammation of the mammary gland that causes major losses in the dairy industry. spp. are among the main agents of this disease. Increased resistance to antibiotics is one of the causes of therapeutic failure. Plants, due to their broad chemodiversity, are an interesting source of new molecules with antibacterial activity. Using these compounds along with traditional antibiotics is a possible method for reversing resistance. The objective of this work was to determine the interactions between the activities of guttiferone-A and 7-epiclusianone, two active substances isolated from the fruits of , and traditional antibiotics against spp. isolated from bovine mastitis and known to be resistant to them. First, the MIC for the antibiotics and bioactive compounds was determined, followed by their activities, alone and in combination. Then, their cytotoxicity was measured in bovine mammary epithelial cells. Finally, molecular docking simulations were performed to elucidate ...

Foodborne pathogens and disease, Jan 29, 2016

The aim of this study was to characterize and compare Staphylococcus spp. isolated from hospitali... more The aim of this study was to characterize and compare Staphylococcus spp. isolated from hospitalized patients and beef marketed in the city of Porto Velho-RO, Brazil. The isolates were subjected to antibiogram tests, adherence capacity tests, detection of the mecA gene, and epidemiological investigation by the random amplified polymorphic DNA (RAPD) technique, using the primers M13 and H12. Among the 123 Staphylococcus spp. isolates, 50 were identified as S. aureus and 73 as coagulase-negative Staphylococcus; among the latter, 7 species were identified. It was observed that the coagulase-negative Staphylococcus isolates showed greater adhesion ability than S. aureus. The profile of antimicrobial susceptibility was different among isolates, all of which were susceptible to vancomycin and linezolid, and had high penicillin resistance rates, varying according to the bacterial class and the source. In this study, all strains were negative for mecA gene detection; however, 36% of S. aure...

Semina: Ciências Agrárias, 2011

O objetivo deste estudo foi avaliar o efeito antimicrobiano de dois diferentes óleos de copaíba s... more O objetivo deste estudo foi avaliar o efeito antimicrobiano de dois diferentes óleos de copaíba sobre isolados de Escherichia coli obtidos de leite mastítico. O ensaio antimicrobiano foi realizado utilizando o teste de difusão em agar, testando três soluções: um controle negativo e duas soluções dos diferentes óleos de copaíba. O teste foi realizado com 27 isolados de E. coli. Oito isolados (29.63%) foram inibidos pela solução com óleo de C. langsdorffii e sete pela solução com óleo de C. officinalis. Os resultados do presente estudo sugerem o óleo de copaiba como uma potencial fonte de novos e seletivos agentes para o tratamento da mastite, após posteriores testes em modelos in vivo.

BioMed Research International, 2014

Shigellosis is a global human health problem and the incidence is highest among children. In the ... more Shigellosis is a global human health problem and the incidence is highest among children. In the present work, mainShigellavirulence genes was examined by PCR and compared to symptoms of pediatric shigellosis. ThirtyShigellaisolates were identified from an etiologic study at which 1,339 children ranging 0–10 years old were enrolled.S. flexneriwas the most frequent species reaching 60.0% of isolates, 22.2% wereS. sonnei, and 6.6% were bothS. dysenteriaeandS. boydii. AllShigellainfected children had diarrhea, but not all were accompanied by others symptoms of bacillary dysentery. Among major virulence genes, the PCR typing revealedipaBCDwas present in all isolates, followed byIpaH7.8,set-1A,set-1B, sen/ospD3, virF,andinvE. The pathogenic potential of the ShET-1B subunit was observed in relation to dehydration (P<0.001) and ShET-2 related to the intestinal injury (P=0.033) evidenced by the presence of bloody diarrhea. Our results show associations among symptoms of shigellosis and v...

BioMed Research International, 2014

This study aims to assess and compare copaiba oleoresin ofCopaifera multijugaand 0.5% silver nitr... more This study aims to assess and compare copaiba oleoresin ofCopaifera multijugaand 0.5% silver nitrate for the induction of pleurodesis in an experimental model. Ninety-six male Wistar rats were divided into three groups: control (0.9% saline solution), copaiba (copaiba oil), and silver nitrate (0.5% silver nitrate). The substances were injected into the right pleural cavity and the alterations were observed macroscopically and microscopically at 24, 48, 72, and 504 h. The value of macroscopic alterations grade and acute inflammatory reaction grade means was higher in the 24 h copaiba group in relation to silver nitrate. Fibrosis and neovascularization means in the visceral pleura were higher in 504 h copaiba group in relation to the silver nitrate group. The grade of the alveolar edema mean was higher in the silver nitrate group in relation to the copaiba group, in which this alteration was not observed. The presence of bronchopneumonia was higher in the 24 h silver nitrate group (n ...

Foodborne Pathogens and Disease, 2014

This study aimed to assess raw milk consumption habits in the urban population of Viçosa, Minas G... more This study aimed to assess raw milk consumption habits in the urban population of Viçosa, Minas Gerais, Brazil, and the microbiological safety and quality of the fluid milk available in retail sales outlets in the same region. A simplified questionnaire regarding raw milk consumption was applied to the persons responsible for food acquisition in 411 residences. The regular consumption of raw milk was observed by 18.5% of the interviewers, and lack of knowledge of possible risks related to this food product. Microbiological safety and quality were assessed for raw (n = 69), pasteurized (n = 80), and ultra-high-temperature (UHT)-treated milk (n = 80) by analyzing the counts of mesophilic aerobes, coliforms, and Escherichia coli, and detection of Listeria monocytogenes and Salmonella spp.; raw milk samples were also subjected to enumeration of coagulasepositive Staphylococcus. Concerning raw milk, 59.4% of the samples were considered as produced in inadequate hygienic conditions, 5.8% of the samples presented counts of coagulase-positive Staphylococcus lower than 100 colony-forming units (CFU)/mL, and no samples presented with positive results for L. monocytogenes or Salmonella spp. All pasteurized and UHT milk samples presented with low counts of mesophilic aerobes and coliforms, while L. monocytogenes and Salmonella spp. were absent. The data demonstrated that raw milk was consumed by the population studied. Despite the absence of potential hazards, raw milk was of poor hygienic quality, in contrast with the processed fluid milk available in retail sales outlets that was safe and of good hygienic quality, highlighting the suitability of pasteurized and UHT milk for human consumption.

Acta Amazonica, 2014

Honeys are described possessing different properties including antimicrobial. Many studies have p... more Honeys are described possessing different properties including antimicrobial. Many studies have presented this activity of honeys produced by Apis mellifera bees, however studies including activities of stingless bees honeys are scarce. The aim of this study was to compare the antimicrobial activity of honeys collected in the Amazonas State from Melipona compressipes, Melipona seminigra and Apis mellifera against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Chromobacterium violaceum, and Candida albicans. Minimum inhibitory concentrations were determined using the agar dilution method with Müller-Hinton agar (for bacteria) or Saboraud agar (for yeast). Staphylococcus aureus and E. faecalis were inhibited by all honeys at concentrations below 12%, while E. coli and C. violaceum were inhibited by stingless bee honeys at concentrations between 10 and 20%. A. mellifera honey inhibited E. coli at a concentration of 7% and Candida violaceum at 0.7%. C. albicans were inh...

Natural Resources Management and Biological Sciences, 2020

Currently, the number of diseases has been increasing and reaching the population directly, and t... more Currently, the number of diseases has been increasing and reaching the population directly, and the deliberate use of drugs is creating resistance of pathogens in several drugs, a fact evidenced by the increased ineffectiveness of drugs and the persistence of infections in the body. Given this, it is necessary to search for new alternative drugs that can effectively promote effective therapy. It is possible to highlight, in Brazil, the diversity of the Amazonian flora, which has several species with considerable potential as a source of new molecules with identified biological activity. Thus, a literature review was conducted in order to describe the applications of some Amazonian extracts and their chemical characteristics and biological activity. The Amazon rain forest has considerable diversity of plant species with biological properties that may be useful to public health. Further research is needed to identify new compounds with health benefits.