F. Angelini - Academia.edu (original) (raw)
Papers by F. Angelini
General and Comparative Endocrinology, 1991
Plasma vitellogenin and 17P-estradiol concentration were determined during the annual reproductiv... more Plasma vitellogenin and 17P-estradiol concentration were determined during the annual reproductive cycle of the female lizard Podarcis s. sicula Raf. living around Naples. Plasma vitellogenin was purified from estrogenized males for characterization and to raise specific immune serum. Using ELISA, plasma vitellogenin titers were determined in relation to ovary weight; plasma 17@-estradiol was measured by RIA method. Native vitellogenin was present as two polypeptide bands: a and g. The electrophoretic patterns, studied in normal male and estrogenized male and female, showed vitellogenin to be a protein present in female and in estrogenized male plasma but not in normal males. Lizard monomeric VTG, determined by SDS-PAGE, was about 200 kDa. Correlations between seasonal ovarian weight variations and plasma vitellogenin and 17S-estradiol suggest that ovarian development in Podarcis depends on plasma vitellogenin synthesis, which in turn relies on plasma estradiol levels. The two ovulatory waves observed in this study coincided with the two peak values of plasma vitellogenin and 17P-estradiol. o 1w1 Academic FWSS. IX.
Experientia, 1983
Arginine-vasotocine (AVT) injected into male adult lizards, Podarcis s. sicula Raf., inhibits the... more Arginine-vasotocine (AVT) injected into male adult lizards, Podarcis s. sicula Raf., inhibits the last phases of spermatogenesis and the endocrine activity of gonads. In female lizards the substance induces an early interruption of egg deposition.
Amphibia-Reptilia, 1989
The oviduct of Tarentola mauritanica from southern Italy can be divided cranio-caudally into 5 re... more The oviduct of Tarentola mauritanica from southern Italy can be divided cranio-caudally into 5 regions: infundibulum that opens into the coelomic cavity, tuba, isthmus, uterus and vagina which opens into the cloaca. The changing morphology of these regions during the reproductive cycle is described. Unlike the male, the genital duct (epididymis) which is fully developed and secretory throughout the year, the oviduct shows a marked annual cycle with maximum activity in May-July. Seasonality would allow optimization of the reproductive effort. This gecko shows three ovulatory waves, namely in late May, June and July; at each time one calcareous cgg is produced per ovary. There is a short period of egg retention in the oviduct. The main breeding period takes place in spring; sometimes a second optional breeding may occur in autumn. Sperm is preserved inside the crypts of the tuba until the next spring.
Bollettino di zoologia, 1986
The evolution of sex steroids in the plasma and gonads of the lizard Podarcis s. sicula during th... more The evolution of sex steroids in the plasma and gonads of the lizard Podarcis s. sicula during the postreproductive period shows that these hormones could be involved in determining refractoriness. In the male, during this phase, the oestradiol could lower the hypothalamo-hypophyseal system through a negative feedback. In the female a similar role could be assigned to progesterone.
Biotechnic & Histochemistry, 2000
We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient b... more We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient bones. The bones examined were fragments of femurs from two human individuals found in the Pompeian C. I. Polybius house and fragments of metacarpals from two horses (Equus sp.) found in the Pompeian "Casti Amanti" house. Both buildings were buried by the 79 A. D. Vesuvius eruption. Fragments of femurs from a modern horse, a modern swine and a modern amphibian also were studied as controls. Some bone sections were stained with two different DNA-specific fluorochromes, 4'-'6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA), while others were stained by the Feulgen reaction. All of the techniques gave a positive reaction within the osteocytic lacunae. Histological analysis of the undecalcified, ground and unstained sections agreed well with results of bone sections stained with either the fluorochromes or the Feulgen reaction. Bones showing good histology also were positive by our DNA-specific stain. Histochemical and histological analyses correlated well with the success of DNA extraction and amplification. Using conventional DNA-specific histochemical techniques in conjunction with histological analysis can be useful in the study of DNA extracted from ancient bone remains while reducing both the amount of time and cost.
Biochemical and Biophysical Research Communications, 1998
Preservation of archaeological asset in Pompeii site requests a systematic planning of conservati... more Preservation of archaeological asset in Pompeii site requests a systematic planning of conservation interventions based on an interdisciplinary knowledge of the built heritage, in the respect of principles of compatibility, reversibility, distinguishability and minimum intervention. Nevertheless, a specific knowledge of constructive techniques used by Roman builders in the ancient Pompeii is still lacking, especially with respect to material mechanical properties. Nowadays, structural interventions are designed based on mechanical properties provided from Italian technical Standard for existing buildings. In this study, mechanical properties of ancient mortars from Pompeii archaeological site are investigated. Eleven specimens of ancient mortars were collected. They come from masonry structures involved in the 79 A.D. Vesuvius' eruption and emerged for the first time as part of the new archaeological excavation works in Regio V, started on May 2018. Furthermore, twelve new lime-based mortars specimens by using two different aggregates were made based on literature provisions about ancient Roman construction techniques. Compressive tests were carried out on ancient and new mortars specimens and the results are herein presented and discussed.
Journal of Cellular Physiology, 2001
A new model of surgical injury for the induction and development of stenosis in common rat caroti... more A new model of surgical injury for the induction and development of stenosis in common rat carotids is described. This model differs from balloon angioplasty or vein graft systems currently applied on animals to develop stenosis, since it involves the entire vessel wall layers and mimics the injury occurring during arterial grafts, endarterectomy or organ transplantation. At different times following arterial damage, the pattern of expression of genes already known to be involved in the proliferation, differentiation, and apoptosis of smooth muscle cells (c-myc, Angiotensin II receptor 1, Bcl-2 and Bax alpha), as well as of Rb and Rb2 genes, whose pattern of expression after arterial injury has not yet been reported, was analyzed by semi-quantitative reverse transcription-polymerase chain reaction technique. Histological and histochemical analysis on carotid sections shows the morphological changes which occurred 30 days after surgical injury in the vessel wall. Molecular and histological data demonstrate that this model of surgical injury induces neointimal proliferation in about 30% of rats. In about 70% of the remaining rats, it induces the processes responsible for negative remodelling, namely the significant accumulation of extracellular matrix and fibers and disorganization of arterial tunics. This model is therefore available for further studies on the expression of genes involved in the arterial stenotic process, as well as for testing drugs aimed at limiting this recurrent pathophysiological phenomenon.
Biochemical and Biophysical Research Communications, 1998
aDNA extraction and amplification procedures have been optimized for Pompeian human bone remains ... more aDNA extraction and amplification procedures have been optimized for Pompeian human bone remains whose diagenesis has been determined by histological analysis. Single copy genes amplification (X and Y amelogenin loci and Y specific alphoid repeat sequences) have been performed and compared with anthropometric data on sexing.
Biotechnic & Histochemistry, 2000
We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient b... more We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient bones. The bones examined were fragments of femurs from two human individuals found in the Pompeian C. I. Polybius house and fragments of metacarpals from two horses (Equus sp.) found in the Pompeian "Casti Amanti" house. Both buildings were buried by the 79 A. D. Vesuvius eruption. Fragments of femurs from a modern horse, a modern swine and a modern amphibian also were studied as controls. Some bone sections were stained with two different DNA-specific fluorochromes, 4'-'6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA), while others were stained by the Feulgen reaction. All of the techniques gave a positive reaction within the osteocytic lacunae. Histological analysis of the undecalcified, ground and unstained sections agreed well with results of bone sections stained with either the fluorochromes or the Feulgen reaction. Bones showing good histology also were positive by our DNA-specific stain. Histochemical and histological analyses correlated well with the success of DNA extraction and amplification. Using conventional DNA-specific histochemical techniques in conjunction with histological analysis can be useful in the study of DNA extracted from ancient bone remains while reducing both the amount of time and cost.
General and Comparative Endocrinology, 1991
Plasma vitellogenin and 17P-estradiol concentration were determined during the annual reproductiv... more Plasma vitellogenin and 17P-estradiol concentration were determined during the annual reproductive cycle of the female lizard Podarcis s. sicula Raf. living around Naples. Plasma vitellogenin was purified from estrogenized males for characterization and to raise specific immune serum. Using ELISA, plasma vitellogenin titers were determined in relation to ovary weight; plasma 17@-estradiol was measured by RIA method. Native vitellogenin was present as two polypeptide bands: a and g. The electrophoretic patterns, studied in normal male and estrogenized male and female, showed vitellogenin to be a protein present in female and in estrogenized male plasma but not in normal males. Lizard monomeric VTG, determined by SDS-PAGE, was about 200 kDa. Correlations between seasonal ovarian weight variations and plasma vitellogenin and 17S-estradiol suggest that ovarian development in Podarcis depends on plasma vitellogenin synthesis, which in turn relies on plasma estradiol levels. The two ovulatory waves observed in this study coincided with the two peak values of plasma vitellogenin and 17P-estradiol. o 1w1 Academic FWSS. IX.
Experientia, 1983
Arginine-vasotocine (AVT) injected into male adult lizards, Podarcis s. sicula Raf., inhibits the... more Arginine-vasotocine (AVT) injected into male adult lizards, Podarcis s. sicula Raf., inhibits the last phases of spermatogenesis and the endocrine activity of gonads. In female lizards the substance induces an early interruption of egg deposition.
Amphibia-Reptilia, 1989
The oviduct of Tarentola mauritanica from southern Italy can be divided cranio-caudally into 5 re... more The oviduct of Tarentola mauritanica from southern Italy can be divided cranio-caudally into 5 regions: infundibulum that opens into the coelomic cavity, tuba, isthmus, uterus and vagina which opens into the cloaca. The changing morphology of these regions during the reproductive cycle is described. Unlike the male, the genital duct (epididymis) which is fully developed and secretory throughout the year, the oviduct shows a marked annual cycle with maximum activity in May-July. Seasonality would allow optimization of the reproductive effort. This gecko shows three ovulatory waves, namely in late May, June and July; at each time one calcareous cgg is produced per ovary. There is a short period of egg retention in the oviduct. The main breeding period takes place in spring; sometimes a second optional breeding may occur in autumn. Sperm is preserved inside the crypts of the tuba until the next spring.
Bollettino di zoologia, 1986
The evolution of sex steroids in the plasma and gonads of the lizard Podarcis s. sicula during th... more The evolution of sex steroids in the plasma and gonads of the lizard Podarcis s. sicula during the postreproductive period shows that these hormones could be involved in determining refractoriness. In the male, during this phase, the oestradiol could lower the hypothalamo-hypophyseal system through a negative feedback. In the female a similar role could be assigned to progesterone.
Biotechnic & Histochemistry, 2000
We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient b... more We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient bones. The bones examined were fragments of femurs from two human individuals found in the Pompeian C. I. Polybius house and fragments of metacarpals from two horses (Equus sp.) found in the Pompeian "Casti Amanti" house. Both buildings were buried by the 79 A. D. Vesuvius eruption. Fragments of femurs from a modern horse, a modern swine and a modern amphibian also were studied as controls. Some bone sections were stained with two different DNA-specific fluorochromes, 4'-'6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA), while others were stained by the Feulgen reaction. All of the techniques gave a positive reaction within the osteocytic lacunae. Histological analysis of the undecalcified, ground and unstained sections agreed well with results of bone sections stained with either the fluorochromes or the Feulgen reaction. Bones showing good histology also were positive by our DNA-specific stain. Histochemical and histological analyses correlated well with the success of DNA extraction and amplification. Using conventional DNA-specific histochemical techniques in conjunction with histological analysis can be useful in the study of DNA extracted from ancient bone remains while reducing both the amount of time and cost.
Biochemical and Biophysical Research Communications, 1998
Preservation of archaeological asset in Pompeii site requests a systematic planning of conservati... more Preservation of archaeological asset in Pompeii site requests a systematic planning of conservation interventions based on an interdisciplinary knowledge of the built heritage, in the respect of principles of compatibility, reversibility, distinguishability and minimum intervention. Nevertheless, a specific knowledge of constructive techniques used by Roman builders in the ancient Pompeii is still lacking, especially with respect to material mechanical properties. Nowadays, structural interventions are designed based on mechanical properties provided from Italian technical Standard for existing buildings. In this study, mechanical properties of ancient mortars from Pompeii archaeological site are investigated. Eleven specimens of ancient mortars were collected. They come from masonry structures involved in the 79 A.D. Vesuvius' eruption and emerged for the first time as part of the new archaeological excavation works in Regio V, started on May 2018. Furthermore, twelve new lime-based mortars specimens by using two different aggregates were made based on literature provisions about ancient Roman construction techniques. Compressive tests were carried out on ancient and new mortars specimens and the results are herein presented and discussed.
Journal of Cellular Physiology, 2001
A new model of surgical injury for the induction and development of stenosis in common rat caroti... more A new model of surgical injury for the induction and development of stenosis in common rat carotids is described. This model differs from balloon angioplasty or vein graft systems currently applied on animals to develop stenosis, since it involves the entire vessel wall layers and mimics the injury occurring during arterial grafts, endarterectomy or organ transplantation. At different times following arterial damage, the pattern of expression of genes already known to be involved in the proliferation, differentiation, and apoptosis of smooth muscle cells (c-myc, Angiotensin II receptor 1, Bcl-2 and Bax alpha), as well as of Rb and Rb2 genes, whose pattern of expression after arterial injury has not yet been reported, was analyzed by semi-quantitative reverse transcription-polymerase chain reaction technique. Histological and histochemical analysis on carotid sections shows the morphological changes which occurred 30 days after surgical injury in the vessel wall. Molecular and histological data demonstrate that this model of surgical injury induces neointimal proliferation in about 30% of rats. In about 70% of the remaining rats, it induces the processes responsible for negative remodelling, namely the significant accumulation of extracellular matrix and fibers and disorganization of arterial tunics. This model is therefore available for further studies on the expression of genes involved in the arterial stenotic process, as well as for testing drugs aimed at limiting this recurrent pathophysiological phenomenon.
Biochemical and Biophysical Research Communications, 1998
aDNA extraction and amplification procedures have been optimized for Pompeian human bone remains ... more aDNA extraction and amplification procedures have been optimized for Pompeian human bone remains whose diagenesis has been determined by histological analysis. Single copy genes amplification (X and Y amelogenin loci and Y specific alphoid repeat sequences) have been performed and compared with anthropometric data on sexing.
Biotechnic & Histochemistry, 2000
We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient b... more We describe histochemical techniques for detecting DNA within the osteocytic lacunae of ancient bones. The bones examined were fragments of femurs from two human individuals found in the Pompeian C. I. Polybius house and fragments of metacarpals from two horses (Equus sp.) found in the Pompeian "Casti Amanti" house. Both buildings were buried by the 79 A. D. Vesuvius eruption. Fragments of femurs from a modern horse, a modern swine and a modern amphibian also were studied as controls. Some bone sections were stained with two different DNA-specific fluorochromes, 4'-'6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA), while others were stained by the Feulgen reaction. All of the techniques gave a positive reaction within the osteocytic lacunae. Histological analysis of the undecalcified, ground and unstained sections agreed well with results of bone sections stained with either the fluorochromes or the Feulgen reaction. Bones showing good histology also were positive by our DNA-specific stain. Histochemical and histological analyses correlated well with the success of DNA extraction and amplification. Using conventional DNA-specific histochemical techniques in conjunction with histological analysis can be useful in the study of DNA extracted from ancient bone remains while reducing both the amount of time and cost.