F. Nabatchian - Academia.edu (original) (raw)

Papers by F. Nabatchian

KAUMS Journal, 2013

Background: Silver is one of the heavy metals in environment and it,s amount in the earth shell i... more Background: Silver is one of the heavy metals in environment and it,s amount in the earth shell is about 0.1 g per ton. Several studies have proven silver nitrate can produce ROS (oxygen reactive species) and harm cells through a variety of mechanisms including interactions with enzymes and proteins sulphydryl groups. Major sources of Silver in surface waters is natural leaching, human activities, such as mining, manufacture of silverware and jewellery, photografic manufacturing and processing which can elevate silver levels in aquatic environments. Materials and Methods: In This study horseradish peroxidase (HRP) activity was determinded under steady-state kinetic conditions. Incubation of the enzyme with 1-100mM silver nitrate for 1-60 min in room temperature resulted in dual effects. Results: Progressive inhibition of the enzymatic activity was observed after 30-60 min enzyme incubation with 0.05-100 mM of Ag+. After 30 and 60 min incubation time, enzyme inhibition was (35-60%) a...

Payavard Salamat, Jan 15, 2013

Payavard Salamat, Jul 15, 2013

Journal of Breast Cancer, 2014

European Journal of Endocrinology, 2000

OBJECTIVE: To investigate in normal-weight premenopausal women the relationship between circulati... more OBJECTIVE: To investigate in normal-weight premenopausal women the relationship between circulating leptin and serum gonadotropins and gonadal steroids, during both spontaneous and pharmacologically induced menstrual cycles. DESIGN: Clinical longitudinal study. METHODS: Two groups of age-matched, normal-weight premenopausal volunteer women (groups I and II) were enrolled in this study. Women in group I were free of any hormonal treatment, while women in group II were taking a triphasic estrogen--progestin contraceptive preparation. Blood samples were collected daily in both groups after an overnight fast throughout a complete menstrual cycle. RESULTS: In the spontaneously cycling women, serum leptin concentration positively correlated with estradiol…

Payavard Salamat, Mar 15, 2015

Objective: The association of ApoE allele frequencies with coronary artery disease (CAD) remains ... more Objective: The association of ApoE allele frequencies with coronary artery disease (CAD) remains unknown in developing countries. Paraoxonase 1 (PON1) is an enzyme that is associated with high density lipoprotein (HDL) and hydrolyzes oxidized lipids in oxidized low density lipoprotein (LDL) and thus protects against the development of atherosclerosis. In this study the association of apoE polymorphism and PON1 with premature CAD was determined. Methodology: The frequency of apo E alleles and the activity of the PON1 among 162 patients with early and late onset CAD was examined. Results: The mean age in the early and late-onset groups were 44.9 ± 4.9 and 71.7±2.5 years respectively. Patients with early-onset CAD had significantly lower serum levels of HDL-C and higher LDL-C/HDL-C ratio than the late-onset group. There was no difference in serum levels of triglyceride, total cholesterol and LDL-C between the two groups. The e2 allele was significantly higher in the late-onset group. 3...

American Journal of Clinical Dermatology, 2016

Increased reactive oxygen species (ROS) and oxidative stress (OS) has been reported in many aller... more Increased reactive oxygen species (ROS) and oxidative stress (OS) has been reported in many allergic and inflammatory skin diseases, including urticaria, psoriasis, and atopic dermatitis (AD). Melatonin is a hormone secreted from the pineal gland and is a potent antioxidant. The aim of the study was to measure serum antioxidant melatonin, oxidants of nitric oxide (NO), and malondialdehyde levels to calculate the serum oxidant-antioxidant balance based on the NO/melatonin and malondialdehyde/melatonin ratios and to determine the correlation with the disease severity in children with AD. Seventy-three children with AD and 67 healthy controls were included in the study. The clinical diagnosis of AD was based on the diagnostic criteria of Hanifin-Rajka. The severity of AD was evaluated by the scoring AD (SCORAD) index, and atopy was determined by skin prick tests (SPTs) with commercial extracts. The OS-related parameters of serum melatonin, NO, malondialdehyde, and the NO/melatonin and malondialdehyde/melatonin ratios were calculated and compared with the results of healthy controls. Serum melatonin levels were higher (p < 0.0001) and serum NO levels and the NO/melatonin and malondialdehyde/melatonin ratios were lower in children with AD than in healthy controls (p = 0.045, p < 0.0001, p < 0.0001, respectively). There was no difference between children with AD and healthy controls in terms of serum malondialdehyde levels (p = 0.119). Serum melatonin levels were significantly lower in severe AD than in mild AD (p = 0.012). However, in terms of serum melatonin levels, there was no difference between mild and moderate AD (p = 0.742) and moderate to severe AD (p = 0.301). There was no significant difference in serum NO and malondialdehyde levels and NO/melatonin and malondialdehyde/melatonin ratios among children with mild, moderate, and severe AD (p > 0.05). A negative correlation was found between serum melatonin levels and the SCORAD index (r = -0.252, p = 0.031), and a positive correlation was found between NO/melatonin and malondialdehyde/melatonin ratios (r = 0.511, p < 0.0001). There was no statistically significant relationship between age (≤24 or >24 months), disease duration (≤6 or >6 months), and sex for the OS-related parameters (p > 0.05). The serum oxidant-antioxidant balance was impaired in children with AD. Serum melatonin levels were higher in children with AD; however, this was negatively correlated with disease severity. Serum NO levels and NO/melatonin and malondialdehyde/melatonin ratios were lower in children with AD than in healthy controls. Melatonin might be used as a promising antioxidant to evaluate disease severity in children with AD. Thus, further studies are needed to clarify the role of melatonin in AD pathogenesis.

Introduction: Oxidative stress has a major role in atherogenesis. There are multiple antioxidants... more Introduction: Oxidative stress has a major role in atherogenesis. There are multiple antioxidants and oxidants in the living organisms. Several studies have shown the effect of oxidative stress in coronary artery disease; So determination of balance between prooxidants and antioxidants which has functional relevance during this disease. In this study the relationship of prooxidant – antioxidant balance (PAB) with coronary artery disease (CAD) has been investigated. Materials and Methods: In this study 68 patients with coronary artery disease and 72 healthy volunteers were included. Prooxidant – antioxidant balace (PAB) assayed based on 3, 3', 5, 5' – tetramethyl – benzidine and its cation as a redox indicator. Also a complete lipid profile and uric acid assay were determind by common biochemical methods for each sample. Results: The PAB values of CAD patients and control group were 70.01 ± 3.36 and 66.40 ± 2.84 respectively. Lipid profile showed significant difference for tr...

Payavard Salamat, Jul 15, 2013

Acta medica Iranica, 2012

Protein- energy malnutrition, wasting and inflammation are frequent complication among patients w... more Protein- energy malnutrition, wasting and inflammation are frequent complication among patients with end-stage renal disease (ESRD). Malnutrition is associated with cardiac co-morbidity, inflammation and poor survival in ESRD patients. Serum albumin is a well-known marker of nutrition in ESRD patients. Serum albumin is still the most commonly used nutritional marker in ESRD patients. C-reactive protein (CRP), the major acute phase response (APR) protein is elevated in these patients. High CRP levels are linked to the degree of atherosclerosis in coronary, peripheral, and extracranial brain arteries. The aim of the present study was to investigate nutritional factor (albumin) and CRP levels in ESRD patients. In this cross- sectional study a total of 300 patients who had ESRD and had been on hemodialysis treatment for at least 6 months were selected. The laboratory tests consisted of measurement of CRP and albumin using high sensitive ELISA kits. The study patients included 157 males ...

Payavard Salamat, Jan 15, 2013

International Journal of Cancer Research, 2009

Blood transfusion = Trasfusione del sangue, 2011

Thalassaemia is a genetic disease in which there is a relative or complete lack of alpha or beta ... more Thalassaemia is a genetic disease in which there is a relative or complete lack of alpha or beta globin chains. Patients with moderate to severe forms of thalassaemia need transfusions from the early years of life. Antibody production against blood group antigens may cause many problems in preparing compatible blood units for transfusion. The identification of definite blood group phenotypes by the haemagglutination method can be difficult because of the mixed population of red blood cells from the donor and recipient. Forty multiply transfused thalassaemic patients and ten healthy controls with no history of blood transfusion were enrolled in this study. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) and haemagglutination methods were used to determine the presence of Rhesus (Rh) C, c, E and e antigens. In this study four primer sets were used for ASO-PCR amplification of RhC/c and RhE/e. Although PCR assays for RhC/c and RHE/e genotyping have been described pr...

KAUMS Journal, 2013

Background: Silver is one of the heavy metals in environment and it,s amount in the earth shell i... more Background: Silver is one of the heavy metals in environment and it,s amount in the earth shell is about 0.1 g per ton. Several studies have proven silver nitrate can produce ROS (oxygen reactive species) and harm cells through a variety of mechanisms including interactions with enzymes and proteins sulphydryl groups. Major sources of Silver in surface waters is natural leaching, human activities, such as mining, manufacture of silverware and jewellery, photografic manufacturing and processing which can elevate silver levels in aquatic environments. Materials and Methods: In This study horseradish peroxidase (HRP) activity was determinded under steady-state kinetic conditions. Incubation of the enzyme with 1-100mM silver nitrate for 1-60 min in room temperature resulted in dual effects. Results: Progressive inhibition of the enzymatic activity was observed after 30-60 min enzyme incubation with 0.05-100 mM of Ag+. After 30 and 60 min incubation time, enzyme inhibition was (35-60%) a...

Payavard Salamat, Jan 15, 2013

Payavard Salamat, Jul 15, 2013

Journal of Breast Cancer, 2014

European Journal of Endocrinology, 2000

OBJECTIVE: To investigate in normal-weight premenopausal women the relationship between circulati... more OBJECTIVE: To investigate in normal-weight premenopausal women the relationship between circulating leptin and serum gonadotropins and gonadal steroids, during both spontaneous and pharmacologically induced menstrual cycles. DESIGN: Clinical longitudinal study. METHODS: Two groups of age-matched, normal-weight premenopausal volunteer women (groups I and II) were enrolled in this study. Women in group I were free of any hormonal treatment, while women in group II were taking a triphasic estrogen--progestin contraceptive preparation. Blood samples were collected daily in both groups after an overnight fast throughout a complete menstrual cycle. RESULTS: In the spontaneously cycling women, serum leptin concentration positively correlated with estradiol…

Payavard Salamat, Mar 15, 2015

Objective: The association of ApoE allele frequencies with coronary artery disease (CAD) remains ... more Objective: The association of ApoE allele frequencies with coronary artery disease (CAD) remains unknown in developing countries. Paraoxonase 1 (PON1) is an enzyme that is associated with high density lipoprotein (HDL) and hydrolyzes oxidized lipids in oxidized low density lipoprotein (LDL) and thus protects against the development of atherosclerosis. In this study the association of apoE polymorphism and PON1 with premature CAD was determined. Methodology: The frequency of apo E alleles and the activity of the PON1 among 162 patients with early and late onset CAD was examined. Results: The mean age in the early and late-onset groups were 44.9 ± 4.9 and 71.7±2.5 years respectively. Patients with early-onset CAD had significantly lower serum levels of HDL-C and higher LDL-C/HDL-C ratio than the late-onset group. There was no difference in serum levels of triglyceride, total cholesterol and LDL-C between the two groups. The e2 allele was significantly higher in the late-onset group. 3...

American Journal of Clinical Dermatology, 2016

Increased reactive oxygen species (ROS) and oxidative stress (OS) has been reported in many aller... more Increased reactive oxygen species (ROS) and oxidative stress (OS) has been reported in many allergic and inflammatory skin diseases, including urticaria, psoriasis, and atopic dermatitis (AD). Melatonin is a hormone secreted from the pineal gland and is a potent antioxidant. The aim of the study was to measure serum antioxidant melatonin, oxidants of nitric oxide (NO), and malondialdehyde levels to calculate the serum oxidant-antioxidant balance based on the NO/melatonin and malondialdehyde/melatonin ratios and to determine the correlation with the disease severity in children with AD. Seventy-three children with AD and 67 healthy controls were included in the study. The clinical diagnosis of AD was based on the diagnostic criteria of Hanifin-Rajka. The severity of AD was evaluated by the scoring AD (SCORAD) index, and atopy was determined by skin prick tests (SPTs) with commercial extracts. The OS-related parameters of serum melatonin, NO, malondialdehyde, and the NO/melatonin and malondialdehyde/melatonin ratios were calculated and compared with the results of healthy controls. Serum melatonin levels were higher (p < 0.0001) and serum NO levels and the NO/melatonin and malondialdehyde/melatonin ratios were lower in children with AD than in healthy controls (p = 0.045, p < 0.0001, p < 0.0001, respectively). There was no difference between children with AD and healthy controls in terms of serum malondialdehyde levels (p = 0.119). Serum melatonin levels were significantly lower in severe AD than in mild AD (p = 0.012). However, in terms of serum melatonin levels, there was no difference between mild and moderate AD (p = 0.742) and moderate to severe AD (p = 0.301). There was no significant difference in serum NO and malondialdehyde levels and NO/melatonin and malondialdehyde/melatonin ratios among children with mild, moderate, and severe AD (p > 0.05). A negative correlation was found between serum melatonin levels and the SCORAD index (r = -0.252, p = 0.031), and a positive correlation was found between NO/melatonin and malondialdehyde/melatonin ratios (r = 0.511, p < 0.0001). There was no statistically significant relationship between age (≤24 or >24 months), disease duration (≤6 or >6 months), and sex for the OS-related parameters (p > 0.05). The serum oxidant-antioxidant balance was impaired in children with AD. Serum melatonin levels were higher in children with AD; however, this was negatively correlated with disease severity. Serum NO levels and NO/melatonin and malondialdehyde/melatonin ratios were lower in children with AD than in healthy controls. Melatonin might be used as a promising antioxidant to evaluate disease severity in children with AD. Thus, further studies are needed to clarify the role of melatonin in AD pathogenesis.

Introduction: Oxidative stress has a major role in atherogenesis. There are multiple antioxidants... more Introduction: Oxidative stress has a major role in atherogenesis. There are multiple antioxidants and oxidants in the living organisms. Several studies have shown the effect of oxidative stress in coronary artery disease; So determination of balance between prooxidants and antioxidants which has functional relevance during this disease. In this study the relationship of prooxidant – antioxidant balance (PAB) with coronary artery disease (CAD) has been investigated. Materials and Methods: In this study 68 patients with coronary artery disease and 72 healthy volunteers were included. Prooxidant – antioxidant balace (PAB) assayed based on 3, 3', 5, 5' – tetramethyl – benzidine and its cation as a redox indicator. Also a complete lipid profile and uric acid assay were determind by common biochemical methods for each sample. Results: The PAB values of CAD patients and control group were 70.01 ± 3.36 and 66.40 ± 2.84 respectively. Lipid profile showed significant difference for tr...

Payavard Salamat, Jul 15, 2013

Acta medica Iranica, 2012

Protein- energy malnutrition, wasting and inflammation are frequent complication among patients w... more Protein- energy malnutrition, wasting and inflammation are frequent complication among patients with end-stage renal disease (ESRD). Malnutrition is associated with cardiac co-morbidity, inflammation and poor survival in ESRD patients. Serum albumin is a well-known marker of nutrition in ESRD patients. Serum albumin is still the most commonly used nutritional marker in ESRD patients. C-reactive protein (CRP), the major acute phase response (APR) protein is elevated in these patients. High CRP levels are linked to the degree of atherosclerosis in coronary, peripheral, and extracranial brain arteries. The aim of the present study was to investigate nutritional factor (albumin) and CRP levels in ESRD patients. In this cross- sectional study a total of 300 patients who had ESRD and had been on hemodialysis treatment for at least 6 months were selected. The laboratory tests consisted of measurement of CRP and albumin using high sensitive ELISA kits. The study patients included 157 males ...

Payavard Salamat, Jan 15, 2013

International Journal of Cancer Research, 2009

Blood transfusion = Trasfusione del sangue, 2011

Thalassaemia is a genetic disease in which there is a relative or complete lack of alpha or beta ... more Thalassaemia is a genetic disease in which there is a relative or complete lack of alpha or beta globin chains. Patients with moderate to severe forms of thalassaemia need transfusions from the early years of life. Antibody production against blood group antigens may cause many problems in preparing compatible blood units for transfusion. The identification of definite blood group phenotypes by the haemagglutination method can be difficult because of the mixed population of red blood cells from the donor and recipient. Forty multiply transfused thalassaemic patients and ten healthy controls with no history of blood transfusion were enrolled in this study. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) and haemagglutination methods were used to determine the presence of Rhesus (Rh) C, c, E and e antigens. In this study four primer sets were used for ASO-PCR amplification of RhC/c and RhE/e. Although PCR assays for RhC/c and RHE/e genotyping have been described pr...