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Papers by Francisco Pérez-alfocea
Plant Physiology and Biochemistry, 2014
Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica × Prunus dulci... more Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica × Prunus dulcis) has been achieved and the action of the regeneration medium on 7 phytohormones, zeatin (Z), zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA), has been studied using High performance liquid chromatography - mass spectrometry (HPLC-MS/MS). Three scion peach cultivars, 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach × almond rootstocks 'Garnem' and 'GF677' were cultured in two different media, Murashige and Skoog supplemented with plant growth regulators (PGRs) (regeneration medium) and without PGRs (control medium), in order to study the effects of the media and/or genotypes in the endogenous hormones content and their role in organogenesis. The highest regeneration rate was obtained with the peach × almond rootstocks and showed a lower content of Z, IAA, ABA, ACC and JA. Only Z, ZR and IAA were affected by the action of the culture media. This study shows which hormones are external PGRs-dependent and what is the weight of the genotype and hormones in peach organogenesis that provide an avenue to manipulate in vitro organogenesis in peach.
Plant Cell Tissue and Organ Culture, 1998
Page 1. Plant Cell, Tissue and Organ Culture 53: 19–26, 1998. © 1998 Kluwer Academic Publishers. ... more Page 1. Plant Cell, Tissue and Organ Culture 53: 19–26, 1998. © 1998 Kluwer Academic Publishers. Printed in the Netherlands. 19 Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture ...
PROTEOMICS, 2013
Salinity is a major threat limiting the productivity of crop plants. A clear demand for improving... more Salinity is a major threat limiting the productivity of crop plants. A clear demand for improving the salinity tolerance of the major crop plants is imposed by the rapidly growing world population. This review summarizes the achievements of proteomic studies to elucidate the response mechanisms of selected model and crop plants to cope with salinity stress. We also aim at identifying research areas, which deserve increased attention in future proteome studies, as a prerequisite to identify novel targets for breeding strategies. Such areas include the impact of plant-microbial communities on the salinity tolerance of crops under field conditions, the importance of hormone signaling in abiotic stress tolerance, and the significance of control mechanisms underlying the observed changes in the proteome patterns. We briefly highlight the impact of novel tools for future proteome studies and argue for the use of integrated approaches. The evaluation of genetic resources by means of novel automated phenotyping facilities will have a large impact on the application of proteomics especially in combination with metabolomics or transcriptomics.
Theoretical and Applied Genetics, 2010
Scientia Horticulturae, 2013
Plant, Cell & Environment, 2010
Plant, Cell & Environment, 2009
Plant Signaling & Behavior, 2010
Plant Science, 1995
The effect of phlorizin, a reversible inhibitor for glucose transporters and that of PCMBS, a non... more The effect of phlorizin, a reversible inhibitor for glucose transporters and that of PCMBS, a non-permeant water soluble chemical modifier of membrane sulphydryl groups, on proline and sugar accumulation and sugar efflux have been studied in tomato leaf discs (Lycopersicon esculentum L. Mill. cv. Volgogradskij) subjected to stress conditions when incubated in 300 mM NaCl or iso-osmotic solution of non-permeant PEG 6000. The amounts of proline and sucrose found in the tissue were mainly related to the structure of the osmoticum used for the treatment: 12.5 and 56 μmol of proline per gram of dry weight were registered under saline and PEG conditions respectively, while 49.4 and 91.7 μmol of sucrose were found under these conditions. The application of inhibitors provoked a different sugar efflux to the bathing medium: PCMBS enhanced the sugars efflux up to 10 times in saline medium and up to three times under PEG conditions. Surprisingly, the sucrose content in the tissue was always similar to that of the same treatment in the absence of the inhibitor. When exogenous sucrose was supplied to the treated leaf discs, it was found that it exerts two opposite effects on proline accumulation since (1) it prevented the inhibitory effect of both phlorizin and PCMBS in some treatments and (2) it enhanced proline accumulation provoked by these inhibitors in control discs and those treated with PEG. The proline response in relation to the accumulation of sugars for osmotic adjustment and the possibility of an osmoregulated process concerning sucrose synthesis and carbohydrate export under stress conditions are discussed.
Plant Science, 1996
The effects of low (control), moderate, and high salinity on sucrose metabolism, in relation to f... more The effects of low (control), moderate, and high salinity on sucrose metabolism, in relation to fruit growth, were assayed in a commercial F 1 tomato hybrid (Lycopersicon esculentum L. Mill) Radja (GC-793). High salinity reduced both fruit growth rate and the mature fruit ...
Plant Cell Reports, 1996
If in vitro culture is to be used for evaluating the salt tolerance of tomato hybrids and segrega... more If in vitro culture is to be used for evaluating the salt tolerance of tomato hybrids and segregant populations in a breeding programme, it is previously necessary to get quick and reliable traits. In this work, growth and physiological responses to salinity of two interspecific hybrids between the cultivated tomato (Lycopersicon esculentum Mill) and its wild salt-tolerant species L pennellii are compared to those of their parents. The leaf callus of the first subculture was grown on media amended with 0, 35, 70, 105, 140, 175 and 210 mM NaCl for 40 days. Relative fresh weight growth of callus in response to increased salinity in the culture medium was much greater in L pennellii than in the tomato cultivars, and greater in the hybrids than in the wild species. Moreover, the different salt tolerance degree of hybrids was related to that of female parents. At high salt levels, only Cl(-) accumulation was higher in L pennellii than in tomato cultivars, whereas in the hybrids both Cl(-), and Na(+) accumulation were higher than in their parents. Proline increased with salinity in the callus of all genotypes; these increases were much higher in the tomato cultivars than in L pennellii, and the hybrids showed a similar response to that of the wild species. Salt-treated callus of the tomato cultivars showed significant increases in valine, isoleucine and leucine contents compared to control callus tissue. In contrast, these amino acids in callus tissues of the wild species and hybrids showed a tendency to decrease with increasing salinity.
Physiologia Plantarum, 1997
The effects of N a Cl on endogenous free levels of the poluamines putrescine, spermi dine and spe... more The effects of N a Cl on endogenous free levels of the poluamines putrescine, spermi dine and spermine, and the relationships between polyamines, K + levels and Na + accumulation were determined in leaves of the cultivated tomato (Lycopersicon esculentum Mill.) and its wild, ...
Physiologia Plantarum, 2003
Physiologia Plantarum, 2013
Physiologia Plantarum, 2009
Plant Physiology and Biochemistry, 2014
Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica × Prunus dulci... more Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica × Prunus dulcis) has been achieved and the action of the regeneration medium on 7 phytohormones, zeatin (Z), zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA), has been studied using High performance liquid chromatography - mass spectrometry (HPLC-MS/MS). Three scion peach cultivars, 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach × almond rootstocks 'Garnem' and 'GF677' were cultured in two different media, Murashige and Skoog supplemented with plant growth regulators (PGRs) (regeneration medium) and without PGRs (control medium), in order to study the effects of the media and/or genotypes in the endogenous hormones content and their role in organogenesis. The highest regeneration rate was obtained with the peach × almond rootstocks and showed a lower content of Z, IAA, ABA, ACC and JA. Only Z, ZR and IAA were affected by the action of the culture media. This study shows which hormones are external PGRs-dependent and what is the weight of the genotype and hormones in peach organogenesis that provide an avenue to manipulate in vitro organogenesis in peach.
Plant Cell Tissue and Organ Culture, 1998
Page 1. Plant Cell, Tissue and Organ Culture 53: 19–26, 1998. © 1998 Kluwer Academic Publishers. ... more Page 1. Plant Cell, Tissue and Organ Culture 53: 19–26, 1998. © 1998 Kluwer Academic Publishers. Printed in the Netherlands. 19 Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture ...
PROTEOMICS, 2013
Salinity is a major threat limiting the productivity of crop plants. A clear demand for improving... more Salinity is a major threat limiting the productivity of crop plants. A clear demand for improving the salinity tolerance of the major crop plants is imposed by the rapidly growing world population. This review summarizes the achievements of proteomic studies to elucidate the response mechanisms of selected model and crop plants to cope with salinity stress. We also aim at identifying research areas, which deserve increased attention in future proteome studies, as a prerequisite to identify novel targets for breeding strategies. Such areas include the impact of plant-microbial communities on the salinity tolerance of crops under field conditions, the importance of hormone signaling in abiotic stress tolerance, and the significance of control mechanisms underlying the observed changes in the proteome patterns. We briefly highlight the impact of novel tools for future proteome studies and argue for the use of integrated approaches. The evaluation of genetic resources by means of novel automated phenotyping facilities will have a large impact on the application of proteomics especially in combination with metabolomics or transcriptomics.
Theoretical and Applied Genetics, 2010
Scientia Horticulturae, 2013
Plant, Cell & Environment, 2010
Plant, Cell & Environment, 2009
Plant Signaling & Behavior, 2010
Plant Science, 1995
The effect of phlorizin, a reversible inhibitor for glucose transporters and that of PCMBS, a non... more The effect of phlorizin, a reversible inhibitor for glucose transporters and that of PCMBS, a non-permeant water soluble chemical modifier of membrane sulphydryl groups, on proline and sugar accumulation and sugar efflux have been studied in tomato leaf discs (Lycopersicon esculentum L. Mill. cv. Volgogradskij) subjected to stress conditions when incubated in 300 mM NaCl or iso-osmotic solution of non-permeant PEG 6000. The amounts of proline and sucrose found in the tissue were mainly related to the structure of the osmoticum used for the treatment: 12.5 and 56 μmol of proline per gram of dry weight were registered under saline and PEG conditions respectively, while 49.4 and 91.7 μmol of sucrose were found under these conditions. The application of inhibitors provoked a different sugar efflux to the bathing medium: PCMBS enhanced the sugars efflux up to 10 times in saline medium and up to three times under PEG conditions. Surprisingly, the sucrose content in the tissue was always similar to that of the same treatment in the absence of the inhibitor. When exogenous sucrose was supplied to the treated leaf discs, it was found that it exerts two opposite effects on proline accumulation since (1) it prevented the inhibitory effect of both phlorizin and PCMBS in some treatments and (2) it enhanced proline accumulation provoked by these inhibitors in control discs and those treated with PEG. The proline response in relation to the accumulation of sugars for osmotic adjustment and the possibility of an osmoregulated process concerning sucrose synthesis and carbohydrate export under stress conditions are discussed.
Plant Science, 1996
The effects of low (control), moderate, and high salinity on sucrose metabolism, in relation to f... more The effects of low (control), moderate, and high salinity on sucrose metabolism, in relation to fruit growth, were assayed in a commercial F 1 tomato hybrid (Lycopersicon esculentum L. Mill) Radja (GC-793). High salinity reduced both fruit growth rate and the mature fruit ...
Plant Cell Reports, 1996
If in vitro culture is to be used for evaluating the salt tolerance of tomato hybrids and segrega... more If in vitro culture is to be used for evaluating the salt tolerance of tomato hybrids and segregant populations in a breeding programme, it is previously necessary to get quick and reliable traits. In this work, growth and physiological responses to salinity of two interspecific hybrids between the cultivated tomato (Lycopersicon esculentum Mill) and its wild salt-tolerant species L pennellii are compared to those of their parents. The leaf callus of the first subculture was grown on media amended with 0, 35, 70, 105, 140, 175 and 210 mM NaCl for 40 days. Relative fresh weight growth of callus in response to increased salinity in the culture medium was much greater in L pennellii than in the tomato cultivars, and greater in the hybrids than in the wild species. Moreover, the different salt tolerance degree of hybrids was related to that of female parents. At high salt levels, only Cl(-) accumulation was higher in L pennellii than in tomato cultivars, whereas in the hybrids both Cl(-), and Na(+) accumulation were higher than in their parents. Proline increased with salinity in the callus of all genotypes; these increases were much higher in the tomato cultivars than in L pennellii, and the hybrids showed a similar response to that of the wild species. Salt-treated callus of the tomato cultivars showed significant increases in valine, isoleucine and leucine contents compared to control callus tissue. In contrast, these amino acids in callus tissues of the wild species and hybrids showed a tendency to decrease with increasing salinity.
Physiologia Plantarum, 1997
The effects of N a Cl on endogenous free levels of the poluamines putrescine, spermi dine and spe... more The effects of N a Cl on endogenous free levels of the poluamines putrescine, spermi dine and spermine, and the relationships between polyamines, K + levels and Na + accumulation were determined in leaves of the cultivated tomato (Lycopersicon esculentum Mill.) and its wild, ...
Physiologia Plantarum, 2003
Physiologia Plantarum, 2013
Physiologia Plantarum, 2009