Fabiana Alovero - Academia.edu (original) (raw)

Papers by Fabiana Alovero

Journal of Chemotherapy, 2010

Carbomer hydrogels 971pNf, 934pNf and 940Nf loaded with ofloxacin were characterized and their an... more Carbomer hydrogels 971pNf, 934pNf and 940Nf loaded with ofloxacin were characterized and their antimicrobial properties evaluated. bactericidal profiles show improved efficacy and prolonged activity exhibited by ofloxacin-containing hydrogels against Pseudomonas aeruginosa. Analysis of bactericidal index (BI) values after a short time of drug exposure confirms the higher potency of hydrogels compared with that of ofloxacin. Increased BI values observed after 24 h indicate prolonged action against the microorganisms evaluated. The bacterial uptake of ofloxacin from hydrogels was higher than that obtained with a solution of free ofloxacin in both fluoroquinolone-sensitive and -resistant P. aeruginosa. The improved uptake in fluoroquinolone-resistant isolates was correlated with the viscosity of hydrogels. The performance of hydrogels seems to be related to their bioadhesive properties that allow prolonged contact time and the release of an effective amount of drug close to bacterial cells. Hence, hydrogels could be used in the development of more effective formulations for topical administration of antibiotics. Improved performance of an old antibiotic can preserve the use of new generation fluoroquinolones.

Objective: To analyze the antimicrobials consumption in a private hospital of third level of care... more Objective: To analyze the antimicrobials consumption in a private hospital of third level of care of Cordoba, Argentina and its evolution between the months of August to November of 2008-2011. Methods: was performed a descriptive cross-sectional study including all patients admitted to hospital with antimicrobial treatment, without excluding pathologies. Data were obtained from the Software Galeno ® of the institution. The consumption was expressed in daily defined doses (DDD) per 100 stays-days. Results: The average consumption was 52.46. Implementing the program for rational use of antimicrobial (P-RUA) in 2009 led to a reduction in consumption that year, with no increase in nosocomial infections. However, since 2010 the DDD/100 stay-day values returned to previous years. Cephalosporins and penicillins accounted for between 68 and 73 % of total consumption of antimicrobials between 2008 and 2011. The first generation cephalosporins contributed to 80% of the consumption of this fam...

Soc Iberoamericana Informacion Cientifica-s I I C, Nov 1, 2013

Archives of Microbiology

Vancomycin (VAN) is unable to penetrate the outer membrane of Gram-negative bacteria and reach th... more Vancomycin (VAN) is unable to penetrate the outer membrane of Gram-negative bacteria and reach the target site. One approach to overcome this limitation is to associate it with compounds with permeabilizing or antimicrobial properties. EudragitE100® (Eu) is a cationic polymer insufficiently characterized for its potential antimicrobial action. Eu-VAN combinations were characterized, the antimicrobial efficacy against Pseudomonas aeruginosa was evaluated and previous studies on the effects of Eu on bacterial envelopes were extended. Time-kill assays showed eradication of P.aeruginosa within 3-6 h exposure to Eu-VAN, whilst VAN was ineffective. Eu showed regrowth in 24 h and delayed colony pigmentation. Although permeabilization of bacterial envelopes or morphological alterations observed by TEM and flow cytometry after exposure to Eu were insufficient to cause bacterial death, they allowed access of VAN to the target site, since Eu-VAN/Van-FL-treated cultures showed fluorescent staining in all bacterial cells, indicating Van-FL internalization. Consequently, Eu potentiated the activity of an otherwise inactive antibiotic against P. aeruginosa. Moreover, Eu-VAN combinations exhibited improved physicochemical properties and could be used in the development of therapeutic alternatives in the treatment of bacterial keratitis.

Antimicrobial Agents and Chemotherapy, 1998

The mode of action of sulfanilyl fluoroquinolones (NSFQs) was investigated with NSFQ-104, NSFQ-10... more The mode of action of sulfanilyl fluoroquinolones (NSFQs) was investigated with NSFQ-104, NSFQ-105, and some structurally related compounds. Evidence arising from interactions withp-aminobenzoic acid and trimethoprim suggested that a sulfonamidelike mechanism of action makes little or no contribution to the in vitro activity of NSFQs. NSFQ-105 showed an activity that inhibits gyrase-catalyzed DNA supercoiling that is similar to the activity of other fluoroquinolones. Also, NSFQ-105 uptake was decreased by the presence of Mg2+and increased by a lower pH. These results indicate that NSFQs having only one ionizable group could exhibit more favorable kinetics of access to the bacterial cell than zwitterionic fluoroquinolones.

The National Program for Human Retroviruses, AIDS and STDs was implemented in the Arturo U. Illia... more The National Program for Human Retroviruses, AIDS and STDs was implemented in the Arturo U. Illia Hospital in 2009. Using the information available in the pharmacy service on patients with syndromic treatment of sexually transmitted infections (STIs), in 2011 a cross-sectional descriptive study was conducted to learn about the situation of the Program records, analyze interventions carried out from the service and contribute to the knowledge of epidemiological trends. STI training activities were implemented during the second half of 2011, and a significant increase in the number of cases was observed at the end of that year. Pregnant women with syphilis and patients hospitalized for other causes were registered in the Program. The diagnosis of trichomoniasis increased significantly, with a higher proportion of women, while syphilis cases remained unchanged. The 21-50 age group was predominant. Although most were residents of Alta Gracia, patients from the area of influence of the H...

Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Sep 7, 2021

This article cites 14 articles, 10 of which can be accessed free at:

Drug delivery and translational research, Feb 1, 2018

This paper builds on a previous paper in which new ciprofloxacin extended-release tablets were de... more This paper builds on a previous paper in which new ciprofloxacin extended-release tablets were developed based on a ciprofloxacin-based swellable drug polyelectrolyte matrix (SDPM-CIP). The matrix contains a molecular dispersion of ciprofloxacin ionically bonded to the acidic groups of carbomer, forming the polyelectrolyte-drug complex CB-CIP. This formulation showed that the release profile of the ciprofloxacin bilayer tablets currently commercialised can be achieved with a simpler strategy. Thus, since ciprofloxacin urine concentrations are associated with the clinical cure of urinary tract infections, the goal of this work was to compare the urinary excretion of SDPM-CIP tablets with those of the CIPRO XR® bilayer tablets. A batch of SDPM-CIP tablets was manufactured by the wet granulation method and the CB-CIP ionic complex was obtained in situ. Fasted healthy volunteers received a single oral dose of 500 mg ciprofloxacin of either formulation in a randomised crossover study. Ur...

This article cites 49 articles, 41 of which can be accessed free

Fil: Rosset, Clarisa Ines. Universidad Nacional de Cordoba. Facultad de Cs.Quimicas. Departamento... more Fil: Rosset, Clarisa Ines. Universidad Nacional de Cordoba. Facultad de Cs.Quimicas. Departamento de Farmacia; Argentina;

European Journal of Pharmaceutical Sciences

Carbohydrate polymers, 2017

The bioadhesive polymeric films as topical drug delivery systems are interesting alternatives to ... more The bioadhesive polymeric films as topical drug delivery systems are interesting alternatives to improve the pharmacotherapy and patient compliances. New derivate biomaterials based on weisocyanate- dendronized PVP- crosslinked chitosan and loaded with ciprofloxacin (CIP), as model drug, were used to prepare bioadhesive films. Relevant in vitro/in vivo attributes to define main physicochemical and biopharmaceutical characteristics for topical wound-healing applications were evaluated. A high proportion of CIP, uniformly dispersed along throughout the film, was loaded. An extended release of CIP and different behaviors of release profiles, depending on the presence of dendron, were observed. The films loaded with CIP were effective in inhibiting the growth of both Gram positive and Gram negative bacteria. In addition, biocompatibility and bioadhesion into conjuntival-sacs of the rabbits suggests that these films have good properties to be applied over skin wounds for topical applicat...

Nanostructures for Oral Medicine, 2017

Materials Science and Engineering: C, 2016

The development and characterization of a novel, gel-type material based on a dendronized polymer... more The development and characterization of a novel, gel-type material based on a dendronized polymer (DP) loaded with ciprofloxacin (CIP), and the evaluation of its possible use for controlled drug release, are presented in this work. DP showed biocompatible and non-toxic behaviors in cultured cells, both of which are considered optimal properties for the design of a final material for biomedical applications. These results were encouraging for the use of the polymer loaded with CIP (as a drug model), under gel form, in the development of a new controlled-release system to be evaluated for topical administration. First, DP-CIP ionic complexes were obtained by an acid-base reaction using the high density of carboxylic acid groups of the DP and the amine groups of the CIP. The complexes obtained in the solid state were broadly characterized using FTIR spectroscopy, XRP diffraction, DSC-TG analysis and optical microscopy techniques. Gels based on the DP-CIP complexes were easily prepared and presented excellent mechanical behaviors. In addition, optimal properties for application on mucosal membranes and skin were achieved due to their high biocompatibility and acute skin non-irritation. Slow and sustained release of CIP toward simulated physiological fluids was observed in the assays (in vitro), attributed to ion exchange phenomenon and to the drug reservoir effect. An in vitro bacterial growth inhibition assay showed significant CIP activity, corresponding to 38 and 58% of that exhibited by a CIP hydrochloride solution at similar CIP concentrations, against Staphylococcus aureus and Pseudomonas aeruginosa, respectively. However, CIP delivery was appropriate, both in terms of magnitude and velocity to allow for a bactericidal effect. In conclusion, the final product showed promising behavior, which could be exploited for the treatment of topical and mucosal opportunistic infections in human or veterinary applications.