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Papers by Fadhel Chaabane

Research paper thumbnail of On-site cellulase production for cost-effective manufacturing of 2nd-generation biofuels

Research paper thumbnail of Process for the Production of an Enzymatic Cocktail Using Solid Residues from a Process for the Biochemical Coversion of Lignocellulosic Materials

Research paper thumbnail of Optimisation of a dedicated cellulolytic enzymatic cocktail using on site available raw materials

Research paper thumbnail of Kinetic Characterization of Trichoderma reesei CL847 TR3002： An Engineered Strain Producing Highly Improved Cellulolytic Cocktail

: Enzymatic hydrolysis of lignocellulose is often considered to be the major economic bottleneck ... more : Enzymatic hydrolysis of lignocellulose is often considered to be the major economic bottleneck of the production process of bioethanol from lignocellulose. It is generally admitted that the most efficient organism for the production of cellulolytic enzymes is the fungus Trichoderma reesei, mostly due to its high secretion capacity. Unfortunately, this fungus secretes very low concentrations of β-glucosidase, thereby often requiring β-glucosidase supplementation for complete cellulose hydrolysis. It is especially important to have sufficient quantities of β-glucosidase in order to prevent inhibition of cellobiohydrolases by cellobiose. In order to optimize the produced cocktail, a more efficient β-glucosidase was cloned into T. reesei CL847 strain. The new strain, called CL847 TR3002, secretes the evolved β-glucosidase and was tested for cellulase production in laboratory-scale reactors. Its growth kinetics and cellulase production were characterized using fed-batch and chemostat m...

Research paper thumbnail of Upgrading the Hemicellulosic Fraction of Biomass into Biofuel

Oil & Gas Science and Technology – Revue d’IFP Energies nouvelles, 2013

Cet article fait partie du dossier thématique ci-dessous publié dans la revue OGST, Vol. 68, n°4,... more Cet article fait partie du dossier thématique ci-dessous publié dans la revue OGST, Vol. 68, n°4, pp. 621-783 et téléchargeable ici D o s s i e r DOSSIER Edited by/Sous la direction de : A. Daudin et A. Quignard PART 1 Second and Third Generation Biofuels: Towards Sustainability and Competitiveness Deuxième et troisième génération de biocarburants : développement durable et compétitivité

Research paper thumbnail of Intensification de la production d'éthanol biocarburant dans un bioréacteur bi-étagé avec recyclage cellulaire: modélisation et stratégie de conduite

... Ezzedine, Arnold, Yvette, Sadki, Sadok, Fatma, toute la famille Ben Chaabane et notamment Tat... more ... Ezzedine, Arnold, Yvette, Sadki, Sadok, Fatma, toute la famille Ben Chaabane et notamment Tata Noura, Khali Elias, Kraiem, Michelle, Hédi, Lassad, Saïda, Mehdi, Radhi et la famille Zouaoui :Ommi Leïla, Faïza, Hager, Houcine, Hassen, Lazhar, Taïeb, Amel, Habiba, Sleh ...

Research paper thumbnail of Scale-down for scale-up of aerobic filamentous fungal fermentations producing cellulases

HAL (Le Centre pour la Communication Scientifique Directe), May 4, 2017

Research paper thumbnail of Scale-up of the culture of filamentous fungi : relationship between agitation, morphology, rheology, mass transfer and fermentation yields

HAL (Le Centre pour la Communication Scientifique Directe), Oct 1, 2017

Research paper thumbnail of Application of the sale‐down methodology to study the effect of mixing on Trichoderma reesei physiology and enzyme production

HAL (Le Centre pour la Communication Scientifique Directe), Sep 15, 2019

Highlights  Trichoderma reesei is sensitive to anaerobiosis  Oxygen gradients in T. reesei cult... more Highlights  Trichoderma reesei is sensitive to anaerobiosis  Oxygen gradients in T. reesei cultures lead to a complex metabolic response  Long exposure of T.reesei to anaerobiosis decrease the cellulases production

Research paper thumbnail of Scale-down approach: tools for the understanding of cellulase production for 2G-bioethanol

HAL (Le Centre pour la Communication Scientifique Directe), May 11, 2015

Research paper thumbnail of The impact of fluid-dynamic stress in stirred tank bioreactors on the synthesis of cellulases by Trichoderma reesei at the intracellular and extracellular levels

Chemical Engineering Science

The impact of fluid-dynamic stress in stirred tank bioreactors on the synthesis of cellulases by ... more The impact of fluid-dynamic stress in stirred tank bioreactors on the synthesis of cellulases by Trichoderma reesei at the intracellular and extracellular levels

Research paper thumbnail of Scale-down studies related to agitation intensity for the scale-up of Trichoderma reesei fermentations producing cellulases in stirred bioreactors

HAL (Le Centre pour la Communication Scientifique Directe), Jul 16, 2017

Research paper thumbnail of Scale-down methodology to study the effect of fluid dynamic stress and oxygen gradients on filamentous fungal fermentations producing cellulases

HAL (Le Centre pour la Communication Scientifique Directe), Nov 29, 2021

Research paper thumbnail of Modeling fixed bed bioreactors for isopropanol and butanol production using Clostridium beijerinckii DSM 6423 immobilized on polyurethane foams

Biochemical Engineering Journal, 2022

Research paper thumbnail of Image Analysis Method for the Characterization of Trichoderma reesei During Fermentations

Trichoderma reesei, 2020

This chapter describes the use of a specific image analysis method with the plug im! software for... more This chapter describes the use of a specific image analysis method with the plug im! software for the characterization of filamentous fungus morphology. It details an application of this method with samples obtained from a fermentation process of a Trichoderma reesei strain. This fully automated and accurate image analysis method provides quantitative and representative data for morphological and topological analyses.

Research paper thumbnail of Rhéologie des champignons filamenteux : un outil pour la compréhension d’un procédé de production de biocatalyseurs utilisés pour la production de bioéthanol

La production d’ethanol a partir de biomasse lignocellulosique necessite la transformation preala... more La production d’ethanol a partir de biomasse lignocellulosique necessite la transformation prealable de cette biomasse en sucres fermentescibles. Cette transformation est realisee par voie biologique grâce a des biocatalyseurs appeles cellulases. La production de ces dernieres represente un verrou economique majeur au developpement du procede. Les cellulases sont generalement produites industriellement par le champignon filamenteux Trichoderma reesei, doue d’une forte capacite secretoire. Ces cultures sont realisees en bioreacteurs aeres et agites mecaniquement qui impliquent la maitrise des conditions physico-chimiques pour etre optimises. Or, la culture correspond a une suspension de champignon dont la viscosite augmente lors de la croissance, avec un comportement rheofluidifiant. Les champignons se developpent en filaments et forment des structures tridimensionnelles allant du micrometre au millimetre rendant la mesure avec un rheometre rotatif a mobile de type plan/plan ou Couet...

Research paper thumbnail of Identification of fluid dynamic stress response of T. reesei during continuous fermentations conducted at high agitation rate

Research paper thumbnail of Viability of mono-specie biofilm formed by the solvent producer Clostridium beijerinckii during continuous fermentation in packed bed bioreactor

Research paper thumbnail of Scale-up agitation criteria for Trichoderma reesei fermentation

Chemical Engineering Science, 2017

Scale-up of aerobic fungal fermentation processes still remains a challenging issue for the biote... more Scale-up of aerobic fungal fermentation processes still remains a challenging issue for the biotechnology industry. This difficulty arises due to the complex interactions between operating conditions (agitation, aeration, etc.), the physicochemical state of the broth (viscosity, the dissolved oxygen concentration, etc.) and the biology of fungi (growth, production, morphology, etc.). Because of their size, filamentous fungi are affected by fluid dynamic stresses but quantification of this complex parameter is a difficult task. In general, indirect criteria are used for the effect of fluid dynamic stresses on scale-up (tip speed, power draw or the energy dissipation/circulation function (EDCF)). In order to better understand the impact of such criteria on the fermentation of the fungus Trichoderma reesei, a wide range of agitation conditions has been explored. The morphology of T. reesei fungus, its specific growth rate and the rheological properties of the broth have all been measured both at bench 2 scale (~ 2.5 L) and for the first time, at a typical commercial scale. These three aspects of the fermentation at both scales were then compared with respect to tip speed, specific power and EDCF. This work has shown that tip speed as a correlator of any of these parameters is totally ineffective whilst the EDCF is clearly the best for extrapolating laboratory data to the commercial scale.

Research paper thumbnail of Process for the Continuous Production of Cellulases by a Filamentous Fungus Using a Carbon Substrate Obtained from an Acid Pretreatment

Research paper thumbnail of On-site cellulase production for cost-effective manufacturing of 2nd-generation biofuels

Research paper thumbnail of Process for the Production of an Enzymatic Cocktail Using Solid Residues from a Process for the Biochemical Coversion of Lignocellulosic Materials

Research paper thumbnail of Optimisation of a dedicated cellulolytic enzymatic cocktail using on site available raw materials

Research paper thumbnail of Kinetic Characterization of Trichoderma reesei CL847 TR3002： An Engineered Strain Producing Highly Improved Cellulolytic Cocktail

: Enzymatic hydrolysis of lignocellulose is often considered to be the major economic bottleneck ... more : Enzymatic hydrolysis of lignocellulose is often considered to be the major economic bottleneck of the production process of bioethanol from lignocellulose. It is generally admitted that the most efficient organism for the production of cellulolytic enzymes is the fungus Trichoderma reesei, mostly due to its high secretion capacity. Unfortunately, this fungus secretes very low concentrations of β-glucosidase, thereby often requiring β-glucosidase supplementation for complete cellulose hydrolysis. It is especially important to have sufficient quantities of β-glucosidase in order to prevent inhibition of cellobiohydrolases by cellobiose. In order to optimize the produced cocktail, a more efficient β-glucosidase was cloned into T. reesei CL847 strain. The new strain, called CL847 TR3002, secretes the evolved β-glucosidase and was tested for cellulase production in laboratory-scale reactors. Its growth kinetics and cellulase production were characterized using fed-batch and chemostat m...

Research paper thumbnail of Upgrading the Hemicellulosic Fraction of Biomass into Biofuel

Oil & Gas Science and Technology – Revue d’IFP Energies nouvelles, 2013

Cet article fait partie du dossier thématique ci-dessous publié dans la revue OGST, Vol. 68, n°4,... more Cet article fait partie du dossier thématique ci-dessous publié dans la revue OGST, Vol. 68, n°4, pp. 621-783 et téléchargeable ici D o s s i e r DOSSIER Edited by/Sous la direction de : A. Daudin et A. Quignard PART 1 Second and Third Generation Biofuels: Towards Sustainability and Competitiveness Deuxième et troisième génération de biocarburants : développement durable et compétitivité

Research paper thumbnail of Intensification de la production d'éthanol biocarburant dans un bioréacteur bi-étagé avec recyclage cellulaire: modélisation et stratégie de conduite

... Ezzedine, Arnold, Yvette, Sadki, Sadok, Fatma, toute la famille Ben Chaabane et notamment Tat... more ... Ezzedine, Arnold, Yvette, Sadki, Sadok, Fatma, toute la famille Ben Chaabane et notamment Tata Noura, Khali Elias, Kraiem, Michelle, Hédi, Lassad, Saïda, Mehdi, Radhi et la famille Zouaoui :Ommi Leïla, Faïza, Hager, Houcine, Hassen, Lazhar, Taïeb, Amel, Habiba, Sleh ...

Research paper thumbnail of Scale-down for scale-up of aerobic filamentous fungal fermentations producing cellulases

HAL (Le Centre pour la Communication Scientifique Directe), May 4, 2017

Research paper thumbnail of Scale-up of the culture of filamentous fungi : relationship between agitation, morphology, rheology, mass transfer and fermentation yields

HAL (Le Centre pour la Communication Scientifique Directe), Oct 1, 2017

Research paper thumbnail of Application of the sale‐down methodology to study the effect of mixing on Trichoderma reesei physiology and enzyme production

HAL (Le Centre pour la Communication Scientifique Directe), Sep 15, 2019

Highlights  Trichoderma reesei is sensitive to anaerobiosis  Oxygen gradients in T. reesei cult... more Highlights  Trichoderma reesei is sensitive to anaerobiosis  Oxygen gradients in T. reesei cultures lead to a complex metabolic response  Long exposure of T.reesei to anaerobiosis decrease the cellulases production

Research paper thumbnail of Scale-down approach: tools for the understanding of cellulase production for 2G-bioethanol

HAL (Le Centre pour la Communication Scientifique Directe), May 11, 2015

Research paper thumbnail of The impact of fluid-dynamic stress in stirred tank bioreactors on the synthesis of cellulases by Trichoderma reesei at the intracellular and extracellular levels

Chemical Engineering Science

The impact of fluid-dynamic stress in stirred tank bioreactors on the synthesis of cellulases by ... more The impact of fluid-dynamic stress in stirred tank bioreactors on the synthesis of cellulases by Trichoderma reesei at the intracellular and extracellular levels

Research paper thumbnail of Scale-down studies related to agitation intensity for the scale-up of Trichoderma reesei fermentations producing cellulases in stirred bioreactors

HAL (Le Centre pour la Communication Scientifique Directe), Jul 16, 2017

Research paper thumbnail of Scale-down methodology to study the effect of fluid dynamic stress and oxygen gradients on filamentous fungal fermentations producing cellulases

HAL (Le Centre pour la Communication Scientifique Directe), Nov 29, 2021

Research paper thumbnail of Modeling fixed bed bioreactors for isopropanol and butanol production using Clostridium beijerinckii DSM 6423 immobilized on polyurethane foams

Biochemical Engineering Journal, 2022

Research paper thumbnail of Image Analysis Method for the Characterization of Trichoderma reesei During Fermentations

Trichoderma reesei, 2020

This chapter describes the use of a specific image analysis method with the plug im! software for... more This chapter describes the use of a specific image analysis method with the plug im! software for the characterization of filamentous fungus morphology. It details an application of this method with samples obtained from a fermentation process of a Trichoderma reesei strain. This fully automated and accurate image analysis method provides quantitative and representative data for morphological and topological analyses.

Research paper thumbnail of Rhéologie des champignons filamenteux : un outil pour la compréhension d’un procédé de production de biocatalyseurs utilisés pour la production de bioéthanol

La production d’ethanol a partir de biomasse lignocellulosique necessite la transformation preala... more La production d’ethanol a partir de biomasse lignocellulosique necessite la transformation prealable de cette biomasse en sucres fermentescibles. Cette transformation est realisee par voie biologique grâce a des biocatalyseurs appeles cellulases. La production de ces dernieres represente un verrou economique majeur au developpement du procede. Les cellulases sont generalement produites industriellement par le champignon filamenteux Trichoderma reesei, doue d’une forte capacite secretoire. Ces cultures sont realisees en bioreacteurs aeres et agites mecaniquement qui impliquent la maitrise des conditions physico-chimiques pour etre optimises. Or, la culture correspond a une suspension de champignon dont la viscosite augmente lors de la croissance, avec un comportement rheofluidifiant. Les champignons se developpent en filaments et forment des structures tridimensionnelles allant du micrometre au millimetre rendant la mesure avec un rheometre rotatif a mobile de type plan/plan ou Couet...

Research paper thumbnail of Identification of fluid dynamic stress response of T. reesei during continuous fermentations conducted at high agitation rate

Research paper thumbnail of Viability of mono-specie biofilm formed by the solvent producer Clostridium beijerinckii during continuous fermentation in packed bed bioreactor

Research paper thumbnail of Scale-up agitation criteria for Trichoderma reesei fermentation

Chemical Engineering Science, 2017

Scale-up of aerobic fungal fermentation processes still remains a challenging issue for the biote... more Scale-up of aerobic fungal fermentation processes still remains a challenging issue for the biotechnology industry. This difficulty arises due to the complex interactions between operating conditions (agitation, aeration, etc.), the physicochemical state of the broth (viscosity, the dissolved oxygen concentration, etc.) and the biology of fungi (growth, production, morphology, etc.). Because of their size, filamentous fungi are affected by fluid dynamic stresses but quantification of this complex parameter is a difficult task. In general, indirect criteria are used for the effect of fluid dynamic stresses on scale-up (tip speed, power draw or the energy dissipation/circulation function (EDCF)). In order to better understand the impact of such criteria on the fermentation of the fungus Trichoderma reesei, a wide range of agitation conditions has been explored. The morphology of T. reesei fungus, its specific growth rate and the rheological properties of the broth have all been measured both at bench 2 scale (~ 2.5 L) and for the first time, at a typical commercial scale. These three aspects of the fermentation at both scales were then compared with respect to tip speed, specific power and EDCF. This work has shown that tip speed as a correlator of any of these parameters is totally ineffective whilst the EDCF is clearly the best for extrapolating laboratory data to the commercial scale.

Research paper thumbnail of Process for the Continuous Production of Cellulases by a Filamentous Fungus Using a Carbon Substrate Obtained from an Acid Pretreatment