Fadila Benayache - Academia.edu (original) (raw)
Papers by Fadila Benayache
Mediterranean Journal of Chemistry, 2014
The aim of this study was to screen various solvent extracts of seeds of Solanum elaeagnifolium t... more The aim of this study was to screen various solvent extracts of seeds of Solanum elaeagnifolium to display the phytochemical composition, the total phenolic content, the total flavonoid content and the antioxidant capacity in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Various extracts of seeds of Solanum elaeagnifolium were obtained by maceration. The total phenolic content of the different extracts was determined by Folin-Ciocalteu method, and the total flavonoid content was quantified using a method based on the formation of a flavonoid–aluminium complex .The antioxidant activity was essayed through some in vitro models such as the antioxidant capacity by phosphomolybdenum method, radical scavenging activity using 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and reducing power assay. The acetone extract showed the highest total phenolics (580.99 ± 20.56 mg gallic acid equivalents/g of extract), and the highest total flav...
Journal of Essential Oil Bearing Plants, Nov 2, 2015
Abstract: The essential oils obtained by steam distillation of aerial parts of Santolina africana... more Abstract: The essential oils obtained by steam distillation of aerial parts of Santolina africana Jord. & Fourr. an endemic species to North Africa and Santolina chamaecyparissus L. growing in the East of Algeria were analyzed by GC and GC-MS. The main components of S. africana were β-pinene (12.78 %), 1,8-cineol (10.02 %), myrcene (6.94 %), curcumene (7.96 %), spathulenol (5.96 %) and β-eudesmol (14.58 %). These results were slightly different from a previous study which reported from the same species: terpinen-4-ol (44 %), borneol (6.73 %), camphor (15.11 %) and α-terpineol (11.31 %). Our results concerning S. chamaecyparissus showed as main components: thujene (2.46 %), camphene (6.84 %), myrcene (6.44 %), 1,8-cineol (11.22 %), terpinen-4-ol ( 4.93 %), spathulenol (7.59 %) and β-eudesmol (3.76 %).
![Research paper thumbnail of A New Synthetic Method of 1,5-Dimethyl-3-Oxabicyclo[3.1.0] Hexane-2,4-Dione](https://attachments.academia-assets.com/108628154/thumbnails/1.jpg)
](Open chemistry journal, Jun 29, 2018
Background: We have described in this reported work a new method in the synthesis of cis-1,5-dime... more Background: We have described in this reported work a new method in the synthesis of cis-1,5-dimethyl-3-oxabicyclo [3.1.0] hexane-2,4-dione in good yield. Objective: Optimization of practical conditions leads to obtain 1,3-cyclopropanedicarboxylic anhydrides as important precursors of functionalized cyclopropane derivatives. Method: The condensation of 2-chloropropanoic acid with ethyl methacrylate using (2M) LDA dissolved in hexane and THF at (-80°C), and the treatment with acetyl chloride permit to obtain the substituted 1,3-cyclopropanedicarboxylic anhydride. Results: We have proceeded to the synthesis of cis-1,5-dimethyl-3-oxabicyclo [3.1.0] hexane-2,4-dione as functionalized organic compound with high efficiency ,taking into account the regioselectivity of carbanion attack to double bond activated by an electrophilic group. Conclusion: Using (2M) LDA dissolved in hexane and THF at (-80°C) is a good way to afford the enantioselective substituted 1,3cyclopropanedicarboxylic anhydrides.
Journal of biologically active products from nature, Jul 4, 2021
Abstract The composition of the essential oil obtained from the dried aerial parts of Senecio mas... more Abstract The composition of the essential oil obtained from the dried aerial parts of Senecio massaicus was analyzed by GC/MS. Twenty-two components have been identified and represented 97.41 % of the total oil composition. The major constituents of the essential oil were m-cymene (30.58 %), n-hexadecanoic acid (14.88 %) and docosane-11-decyl (10.43 %). Four methods were used to determine the antioxidant activity: DPPH, ABTS, CUPRAC and reducing power assay. The results indicate that the essential oil extract has moderate to low activity compared to the reference antioxidant compounds. In vitro anticholinesterase activity of the essential oil has also been studied. It exhibited higher inhibitory activity against butyrylcholinesterase (BChE) than against acetylcholinesterase (AChE). Docking studies conducted for Alzheimer's disease-related enzymes have displayed that compounds docosane-11-decyl and octaethyleneglycol monododecyl ether have strong potency, and compounds 15,15’Bi1,4,7,10,13-pentaoxacyclohexadecane and n-Hexadecanoic acid have moderate inhibitory potential. In addition, these three compounds (Docosane-11-decyl, octaethyleneglycol monododecyl ether and 15,15’Bi1,4,7,10,13-pentaoxacyclohexadecane) of the essential oil displayed strong interaction against SARS-CoV-2 main protease and Nsp15 endoribonuclease. Therefore, it could be useful to provide anticholinesterase agent and anti-coronavirus candidate drugs.
Natural Product Research, Feb 12, 2015
HAL (Le Centre pour la Communication Scientifique Directe), 2014
The genus Haplophyllum A. Juss. (Rutaceae) consists of 70 species found mainly in warm, temperate... more The genus Haplophyllum A. Juss. (Rutaceae) consists of 70 species found mainly in warm, temperate, and subtropical regions of the northern hemisphere of the Old World [1, 2]. This genus, with the local name of "Sodabi," is represented in the flora of Iran by 30 species, of which 14 are endemic [3]. Various members of the genus are used in traditional medicine for the treatment of gynecological disorders, malaria, rheumatoid arthritis, herpes, warts, erysipelas, toothache, stomachache, skin diseases, and in the treatment of testicular cancer [4-6]. In the earliest scientific sources, such as the Canon Medicinae by Avicenna, it is indicated that Haplophyllum species can be used for treating different diseases [7, 8].
International journal of pharmacognosy and phytochemical research, Jan 15, 2017
The essential oil of the aerial parts of Centaurea sempervirens L. (Asteraceae), synonym : Cheiro... more The essential oil of the aerial parts of Centaurea sempervirens L. (Asteraceae), synonym : Cheirolophus sempervirens (L.) Pomel, was obtained by steam distillation and analyzed by GC-FID and GC-MS. 30 components were identified corresponding to 78.5% of the total oil. Among the identified constituents, oxygenated compounds represented 33.4%, from which 21.2% were hydrocarbons, 10.7% were sesquiterpenes. The non oxygenated compounds were hydrocarbons (9.8%). Phthalates represented 35.3% of the total oil. The major components were 6,10,14-trimethylpentadecan-2-one (12.4%) and epi-torilenol (5.1%). This is the first report on the chemical composition of the essential oil of this species.
Der Pharmacia Lettre, 2016
This study was conducted to determine the total flavonoid and flavonol contents and the estimatio... more This study was conducted to determine the total flavonoid and flavonol contents and the estimation of antioxidant activity by DPPH radical scavenging effect of crude ethanolic extracts of the aerial parts of four Algerian medicinal and aromatic plants : Juniperus oxycedrus L., Juniperus phoenicea L. (Cupressaceae), Cedrus atlantica Manetti (Pinaceae) and Marrubium vulgare L. (Lamiaceae). Total flavonoid and flavonol contents in these extracts were determined using AlCl 3 method and their amount calculated as quercetin µEQ/mg. Synthetic antioxidants such as rutin and BHA were used as standard. The results of antioxidant activity by DPPH radical scavenging indicated better activities for Cedrus atlantica Manetti and Marrubium vulgare L.
In the present study, we report the following: isolation and structural elucidation of two previo... more In the present study, we report the following: isolation and structural elucidation of two previously unknown in Thymelaea microphylla compounds, ethyl caffeate 1 and ladanein 2; evaluation of antioxidant properties and total phenolic content (TPC) of T. microphylla extracts; photoisomerization of cis/trans-tilirosides and structureantioxidant activity relationship of these two isomers. The petroleum ether and chloroform extracts were tested by DPPH • , ABTS •+ scavenging capacity (RSC) and ferric reducing antioxidant power (FRAP) assays. The results showed that there is a strong correlation between TPC and antioxidant activity of different T. microphylla extracts. Photoisomerization was induced by UV lamps at 365 nm, the percentage of cis-tiliroside was observed to increase up to 30%. Antioxidant activity was found to increase after phtoisomerization of tiliroside.
Parasitology International, Jun 1, 2021
Protozoan pathogens that cause neglected tropical diseases are a major public health concern in t... more Protozoan pathogens that cause neglected tropical diseases are a major public health concern in tropical and developing countries. In the course of our ongoing search for new lead compounds as potential antiprotozoal agents, this study aims to perform a bio-guided fractionation of Pituranthos battandieri, using an in vitro assay against Leishmania amazonensis and Trypanosoma cruzi. Two known polyacetylenes, (-)-panaxydiol (1) and (-)-falcarindiol (2) were identified from the ethanolic extract of the aerial parts of P. battandieri as the main bioactive constituents. Compounds 1 and 2 showed similar potency (IC50 values of 5.76 and 5.68 μM, respectively) against L. amazonensis to miltefosine (IC50 value of 6.48 μM), the reference drug, and low toxicity on macrophage cell lines J774. Moreover, compound 1 exhibited moderate activity (IC50 23.24 μM) against T. cruzi. In addition, three known furanocoumarins, 8-geranyloxypsoralen (3), 8-geranyloxy-5-methoxypsoralen (4), and phellopterin (5) were isolated. Their structures were elucidated by NMR and MS analysis. Compounds 1 and 2 are described for the first time in the Pituranthos genus, and this is the first report on their antiprotozoal activity. These results highlight this type of polyacetylenes as an interesting scaffold for the development of novel antiparasitic drugs.
Acta Crystallographica Section E-structure Reports Online, Jul 15, 2009
Biochemical Systematics and Ecology, Aug 1, 2007
Combinatorial Chemistry & High Throughput Screening, Dec 20, 2016
The polyphenolic compounds in wines are beneficial to human health. To better understand the effe... more The polyphenolic compounds in wines are beneficial to human health. To better understand the effect of different factors on the phenolics and antioxidant activities of wine during rice wine fermentation, 6 rice materials and 7 rice wine starters from rural areas were used to ferment Chinese rice wine. The results showed that black rice and the starter O4 were preferred for the optimum polyphenolic content and antioxidant activity, respectively. In addition, the results indicated that the polyphenolics of the rice wines were mainly derived from their plant materials and that various rice materials could result in drastically different wine quality. In contrast, the total polyphenol contents could be less significantly changed by using different starters. The antioxidant activity results of these fermented wines were consistent with the results of the total polyphenol contents. The rice wines produced using 6 different rice materials were significantly different in antioxidant activity measured by ABTS and FRAP assay. Similar to the results of the total polyphenol content and the antioxidant activity, the wine samples with different rice materials showed significantly different polyphenolic profiles compared with those of the rice wine group with different starters. These results suggested that rice materials and starters have different effects on polyphenol extraction and conversion of rice wines.
Molecules, Aug 6, 2018
A method using high-performance liquid chromatography coupled with a photodiode array detector wa... more A method using high-performance liquid chromatography coupled with a photodiode array detector was proposed for the rapid characterization of different phenolic constituents from the extracts of Atriplex mollis aerial parts. Atriplex species are known for their multiple biological activities, but no information is available in the literature about A. mollis. With the aim to firstly characterize the main secondary metabolites of this plant, so as to orient better the biological evaluation, we applied three different extraction procedures and compared the chromatographic results. Microwave-assisted extraction gave the best yield and recovery of important compounds such as gallic acid, catechin, chlorogenic acid, p-OH benzoic acid, rutin, sinapinic acid, t-ferulic acid, naringenin and benzoic acid. These constituents belong to three important chemical classes: phenolic acids, flavonoids and monoterpenes. Color evaluation and analysis of chlorophylls (a and b) and carotenoids complete the preliminary profile of this plant. From these analyses, Atriplex mollis is a source of bioactive compounds (especially rutin, t-ferulic acid and gallic acid) and could be recommended as a plant of phyto-pharmaceutical relevance, opening new perspectives on this salt-tolerant plant.
Acta Scientifica Naturalis, Nov 8, 2019
The essential oil of the aerial parts of Thymus ciliatus (Desf.
Chemistry of Natural Compounds, Mar 1, 2011
As a part of our ongoing program of research on Algerian Asteraceae [1-4], we report our results ... more As a part of our ongoing program of research on Algerian Asteraceae [1-4], we report our results on Volutaria crupinoides (Desf.) Maire (Asteraceae), an endemic species to the Sahara [5]. This work concerned the phytochemical study of the chloroform and n-butanol soluble parts of the aqueous-ethanol extract of the leaves and flowers of Volutaria crupinoides. Volutaria crupinoides was collected during the flowering stage in May 1998 in the Algerian Sahara and was authenticated by Pr. A. Kaabeche (University of Setif Algeria). Air-dried leaves and flowers (1576 g) of Volutaria crupinoides were macerated in an EtOH solution (70%) for 24 hours three times. After filtration, the filtrate was concentrated (500 mL) and dissolved in H 2 O (600 mL). The resulting solution was extracted successively with CHCl 3 and n-butanol. The organic phases were dried with Na 2 SO 4 filtered, and concentrated under low pressure at room temperature to obtain the extracts: chloroform (12.4 g) and n-butanol (25.4 g). The chloroform extract (6 g) was chromatographed on a silica gel (70-230 mesh) column eluted with a gradient of hexane-EtOAc to yield 12 fractions (F 1-F 12). Fraction F 6 (n-hexane-EtOAc 90:10) was subjected to preparative TLC on silica gel (n-hexane-EtOAc 50:50) to give compound 1 (55 mg). The n-BuOH extract (14 g) was applied to a polyamid SC6 column eluted with a gradient of toluene-MeOH to yield 15 fractions (F 1-F 15). Fraction F 6 (toluene-MeOH 80:20) was purified by preparative TLC on polyamide (toluene-MeOHmethyl ethyl ketone 4:3:3) to give compound 2. Fraction F 9 (toluene-MeOH 70:30) was submitted to preparative TLC on polyamide (water-MeOH-methyl ethyl ketone-acetylacetone 13:3:3:1) followed by purification over Sephadex LH-20 column using MeOH as eluent to give compounds 3 and 4. The structures were established by chemical and spectral analysis, mainly FAB-MS (+), IR, UV, 1 H NMR, 13 C NMR, and NMR experiments (DEPT, COSY, HSQC, and HMBC). All these data were in good agreement with the respective literature data [6-11].
Chemistry of Natural Compounds, Mar 1, 2005
Constantine, Algerie, Fax: (213) 31 62 49 12, e-mail: medjroubi@yahoo.fr; 2) Laboratoire de Pharm... more Constantine, Algerie, Fax: (213) 31 62 49 12, e-mail: medjroubi@yahoo.fr; 2) Laboratoire de Pharmacognosie UFR de medecine et de Pharmacie, 22 Boulevard Gambetta, 76183 Rouen Cedex 1; 3) Laboratoire de Pharmacognosie, Faculte de Pharmacie, Universite Rene Descartes Paris V, France. Published in Khimiya Prirodnykh Soedinenii, No. 2, p. 180, March-April, 2005. Original article submitted August 18, 2004.
Mediterranean Journal of Chemistry, 2014
The aim of this study was to screen various solvent extracts of seeds of Solanum elaeagnifolium t... more The aim of this study was to screen various solvent extracts of seeds of Solanum elaeagnifolium to display the phytochemical composition, the total phenolic content, the total flavonoid content and the antioxidant capacity in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Various extracts of seeds of Solanum elaeagnifolium were obtained by maceration. The total phenolic content of the different extracts was determined by Folin-Ciocalteu method, and the total flavonoid content was quantified using a method based on the formation of a flavonoid–aluminium complex .The antioxidant activity was essayed through some in vitro models such as the antioxidant capacity by phosphomolybdenum method, radical scavenging activity using 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and reducing power assay. The acetone extract showed the highest total phenolics (580.99 ± 20.56 mg gallic acid equivalents/g of extract), and the highest total flav...
Journal of Essential Oil Bearing Plants, Nov 2, 2015
Abstract: The essential oils obtained by steam distillation of aerial parts of Santolina africana... more Abstract: The essential oils obtained by steam distillation of aerial parts of Santolina africana Jord. & Fourr. an endemic species to North Africa and Santolina chamaecyparissus L. growing in the East of Algeria were analyzed by GC and GC-MS. The main components of S. africana were β-pinene (12.78 %), 1,8-cineol (10.02 %), myrcene (6.94 %), curcumene (7.96 %), spathulenol (5.96 %) and β-eudesmol (14.58 %). These results were slightly different from a previous study which reported from the same species: terpinen-4-ol (44 %), borneol (6.73 %), camphor (15.11 %) and α-terpineol (11.31 %). Our results concerning S. chamaecyparissus showed as main components: thujene (2.46 %), camphene (6.84 %), myrcene (6.44 %), 1,8-cineol (11.22 %), terpinen-4-ol ( 4.93 %), spathulenol (7.59 %) and β-eudesmol (3.76 %).
Open chemistry journal, Jun 29, 2018
Background: We have described in this reported work a new method in the synthesis of cis-1,5-dime... more Background: We have described in this reported work a new method in the synthesis of cis-1,5-dimethyl-3-oxabicyclo [3.1.0] hexane-2,4-dione in good yield. Objective: Optimization of practical conditions leads to obtain 1,3-cyclopropanedicarboxylic anhydrides as important precursors of functionalized cyclopropane derivatives. Method: The condensation of 2-chloropropanoic acid with ethyl methacrylate using (2M) LDA dissolved in hexane and THF at (-80°C), and the treatment with acetyl chloride permit to obtain the substituted 1,3-cyclopropanedicarboxylic anhydride. Results: We have proceeded to the synthesis of cis-1,5-dimethyl-3-oxabicyclo [3.1.0] hexane-2,4-dione as functionalized organic compound with high efficiency ,taking into account the regioselectivity of carbanion attack to double bond activated by an electrophilic group. Conclusion: Using (2M) LDA dissolved in hexane and THF at (-80°C) is a good way to afford the enantioselective substituted 1,3cyclopropanedicarboxylic anhydrides.
Journal of biologically active products from nature, Jul 4, 2021
Abstract The composition of the essential oil obtained from the dried aerial parts of Senecio mas... more Abstract The composition of the essential oil obtained from the dried aerial parts of Senecio massaicus was analyzed by GC/MS. Twenty-two components have been identified and represented 97.41 % of the total oil composition. The major constituents of the essential oil were m-cymene (30.58 %), n-hexadecanoic acid (14.88 %) and docosane-11-decyl (10.43 %). Four methods were used to determine the antioxidant activity: DPPH, ABTS, CUPRAC and reducing power assay. The results indicate that the essential oil extract has moderate to low activity compared to the reference antioxidant compounds. In vitro anticholinesterase activity of the essential oil has also been studied. It exhibited higher inhibitory activity against butyrylcholinesterase (BChE) than against acetylcholinesterase (AChE). Docking studies conducted for Alzheimer's disease-related enzymes have displayed that compounds docosane-11-decyl and octaethyleneglycol monododecyl ether have strong potency, and compounds 15,15’Bi1,4,7,10,13-pentaoxacyclohexadecane and n-Hexadecanoic acid have moderate inhibitory potential. In addition, these three compounds (Docosane-11-decyl, octaethyleneglycol monododecyl ether and 15,15’Bi1,4,7,10,13-pentaoxacyclohexadecane) of the essential oil displayed strong interaction against SARS-CoV-2 main protease and Nsp15 endoribonuclease. Therefore, it could be useful to provide anticholinesterase agent and anti-coronavirus candidate drugs.
Natural Product Research, Feb 12, 2015
HAL (Le Centre pour la Communication Scientifique Directe), 2014
The genus Haplophyllum A. Juss. (Rutaceae) consists of 70 species found mainly in warm, temperate... more The genus Haplophyllum A. Juss. (Rutaceae) consists of 70 species found mainly in warm, temperate, and subtropical regions of the northern hemisphere of the Old World [1, 2]. This genus, with the local name of "Sodabi," is represented in the flora of Iran by 30 species, of which 14 are endemic [3]. Various members of the genus are used in traditional medicine for the treatment of gynecological disorders, malaria, rheumatoid arthritis, herpes, warts, erysipelas, toothache, stomachache, skin diseases, and in the treatment of testicular cancer [4-6]. In the earliest scientific sources, such as the Canon Medicinae by Avicenna, it is indicated that Haplophyllum species can be used for treating different diseases [7, 8].
International journal of pharmacognosy and phytochemical research, Jan 15, 2017
The essential oil of the aerial parts of Centaurea sempervirens L. (Asteraceae), synonym : Cheiro... more The essential oil of the aerial parts of Centaurea sempervirens L. (Asteraceae), synonym : Cheirolophus sempervirens (L.) Pomel, was obtained by steam distillation and analyzed by GC-FID and GC-MS. 30 components were identified corresponding to 78.5% of the total oil. Among the identified constituents, oxygenated compounds represented 33.4%, from which 21.2% were hydrocarbons, 10.7% were sesquiterpenes. The non oxygenated compounds were hydrocarbons (9.8%). Phthalates represented 35.3% of the total oil. The major components were 6,10,14-trimethylpentadecan-2-one (12.4%) and epi-torilenol (5.1%). This is the first report on the chemical composition of the essential oil of this species.
Der Pharmacia Lettre, 2016
This study was conducted to determine the total flavonoid and flavonol contents and the estimatio... more This study was conducted to determine the total flavonoid and flavonol contents and the estimation of antioxidant activity by DPPH radical scavenging effect of crude ethanolic extracts of the aerial parts of four Algerian medicinal and aromatic plants : Juniperus oxycedrus L., Juniperus phoenicea L. (Cupressaceae), Cedrus atlantica Manetti (Pinaceae) and Marrubium vulgare L. (Lamiaceae). Total flavonoid and flavonol contents in these extracts were determined using AlCl 3 method and their amount calculated as quercetin µEQ/mg. Synthetic antioxidants such as rutin and BHA were used as standard. The results of antioxidant activity by DPPH radical scavenging indicated better activities for Cedrus atlantica Manetti and Marrubium vulgare L.
In the present study, we report the following: isolation and structural elucidation of two previo... more In the present study, we report the following: isolation and structural elucidation of two previously unknown in Thymelaea microphylla compounds, ethyl caffeate 1 and ladanein 2; evaluation of antioxidant properties and total phenolic content (TPC) of T. microphylla extracts; photoisomerization of cis/trans-tilirosides and structureantioxidant activity relationship of these two isomers. The petroleum ether and chloroform extracts were tested by DPPH • , ABTS •+ scavenging capacity (RSC) and ferric reducing antioxidant power (FRAP) assays. The results showed that there is a strong correlation between TPC and antioxidant activity of different T. microphylla extracts. Photoisomerization was induced by UV lamps at 365 nm, the percentage of cis-tiliroside was observed to increase up to 30%. Antioxidant activity was found to increase after phtoisomerization of tiliroside.
Parasitology International, Jun 1, 2021
Protozoan pathogens that cause neglected tropical diseases are a major public health concern in t... more Protozoan pathogens that cause neglected tropical diseases are a major public health concern in tropical and developing countries. In the course of our ongoing search for new lead compounds as potential antiprotozoal agents, this study aims to perform a bio-guided fractionation of Pituranthos battandieri, using an in vitro assay against Leishmania amazonensis and Trypanosoma cruzi. Two known polyacetylenes, (-)-panaxydiol (1) and (-)-falcarindiol (2) were identified from the ethanolic extract of the aerial parts of P. battandieri as the main bioactive constituents. Compounds 1 and 2 showed similar potency (IC50 values of 5.76 and 5.68 μM, respectively) against L. amazonensis to miltefosine (IC50 value of 6.48 μM), the reference drug, and low toxicity on macrophage cell lines J774. Moreover, compound 1 exhibited moderate activity (IC50 23.24 μM) against T. cruzi. In addition, three known furanocoumarins, 8-geranyloxypsoralen (3), 8-geranyloxy-5-methoxypsoralen (4), and phellopterin (5) were isolated. Their structures were elucidated by NMR and MS analysis. Compounds 1 and 2 are described for the first time in the Pituranthos genus, and this is the first report on their antiprotozoal activity. These results highlight this type of polyacetylenes as an interesting scaffold for the development of novel antiparasitic drugs.
Acta Crystallographica Section E-structure Reports Online, Jul 15, 2009
Biochemical Systematics and Ecology, Aug 1, 2007
Combinatorial Chemistry & High Throughput Screening, Dec 20, 2016
The polyphenolic compounds in wines are beneficial to human health. To better understand the effe... more The polyphenolic compounds in wines are beneficial to human health. To better understand the effect of different factors on the phenolics and antioxidant activities of wine during rice wine fermentation, 6 rice materials and 7 rice wine starters from rural areas were used to ferment Chinese rice wine. The results showed that black rice and the starter O4 were preferred for the optimum polyphenolic content and antioxidant activity, respectively. In addition, the results indicated that the polyphenolics of the rice wines were mainly derived from their plant materials and that various rice materials could result in drastically different wine quality. In contrast, the total polyphenol contents could be less significantly changed by using different starters. The antioxidant activity results of these fermented wines were consistent with the results of the total polyphenol contents. The rice wines produced using 6 different rice materials were significantly different in antioxidant activity measured by ABTS and FRAP assay. Similar to the results of the total polyphenol content and the antioxidant activity, the wine samples with different rice materials showed significantly different polyphenolic profiles compared with those of the rice wine group with different starters. These results suggested that rice materials and starters have different effects on polyphenol extraction and conversion of rice wines.
Molecules, Aug 6, 2018
A method using high-performance liquid chromatography coupled with a photodiode array detector wa... more A method using high-performance liquid chromatography coupled with a photodiode array detector was proposed for the rapid characterization of different phenolic constituents from the extracts of Atriplex mollis aerial parts. Atriplex species are known for their multiple biological activities, but no information is available in the literature about A. mollis. With the aim to firstly characterize the main secondary metabolites of this plant, so as to orient better the biological evaluation, we applied three different extraction procedures and compared the chromatographic results. Microwave-assisted extraction gave the best yield and recovery of important compounds such as gallic acid, catechin, chlorogenic acid, p-OH benzoic acid, rutin, sinapinic acid, t-ferulic acid, naringenin and benzoic acid. These constituents belong to three important chemical classes: phenolic acids, flavonoids and monoterpenes. Color evaluation and analysis of chlorophylls (a and b) and carotenoids complete the preliminary profile of this plant. From these analyses, Atriplex mollis is a source of bioactive compounds (especially rutin, t-ferulic acid and gallic acid) and could be recommended as a plant of phyto-pharmaceutical relevance, opening new perspectives on this salt-tolerant plant.
Acta Scientifica Naturalis, Nov 8, 2019
The essential oil of the aerial parts of Thymus ciliatus (Desf.
Chemistry of Natural Compounds, Mar 1, 2011
As a part of our ongoing program of research on Algerian Asteraceae [1-4], we report our results ... more As a part of our ongoing program of research on Algerian Asteraceae [1-4], we report our results on Volutaria crupinoides (Desf.) Maire (Asteraceae), an endemic species to the Sahara [5]. This work concerned the phytochemical study of the chloroform and n-butanol soluble parts of the aqueous-ethanol extract of the leaves and flowers of Volutaria crupinoides. Volutaria crupinoides was collected during the flowering stage in May 1998 in the Algerian Sahara and was authenticated by Pr. A. Kaabeche (University of Setif Algeria). Air-dried leaves and flowers (1576 g) of Volutaria crupinoides were macerated in an EtOH solution (70%) for 24 hours three times. After filtration, the filtrate was concentrated (500 mL) and dissolved in H 2 O (600 mL). The resulting solution was extracted successively with CHCl 3 and n-butanol. The organic phases were dried with Na 2 SO 4 filtered, and concentrated under low pressure at room temperature to obtain the extracts: chloroform (12.4 g) and n-butanol (25.4 g). The chloroform extract (6 g) was chromatographed on a silica gel (70-230 mesh) column eluted with a gradient of hexane-EtOAc to yield 12 fractions (F 1-F 12). Fraction F 6 (n-hexane-EtOAc 90:10) was subjected to preparative TLC on silica gel (n-hexane-EtOAc 50:50) to give compound 1 (55 mg). The n-BuOH extract (14 g) was applied to a polyamid SC6 column eluted with a gradient of toluene-MeOH to yield 15 fractions (F 1-F 15). Fraction F 6 (toluene-MeOH 80:20) was purified by preparative TLC on polyamide (toluene-MeOHmethyl ethyl ketone 4:3:3) to give compound 2. Fraction F 9 (toluene-MeOH 70:30) was submitted to preparative TLC on polyamide (water-MeOH-methyl ethyl ketone-acetylacetone 13:3:3:1) followed by purification over Sephadex LH-20 column using MeOH as eluent to give compounds 3 and 4. The structures were established by chemical and spectral analysis, mainly FAB-MS (+), IR, UV, 1 H NMR, 13 C NMR, and NMR experiments (DEPT, COSY, HSQC, and HMBC). All these data were in good agreement with the respective literature data [6-11].
Chemistry of Natural Compounds, Mar 1, 2005
Constantine, Algerie, Fax: (213) 31 62 49 12, e-mail: medjroubi@yahoo.fr; 2) Laboratoire de Pharm... more Constantine, Algerie, Fax: (213) 31 62 49 12, e-mail: medjroubi@yahoo.fr; 2) Laboratoire de Pharmacognosie UFR de medecine et de Pharmacie, 22 Boulevard Gambetta, 76183 Rouen Cedex 1; 3) Laboratoire de Pharmacognosie, Faculte de Pharmacie, Universite Rene Descartes Paris V, France. Published in Khimiya Prirodnykh Soedinenii, No. 2, p. 180, March-April, 2005. Original article submitted August 18, 2004.