Falguni Modi - Academia.edu (original) (raw)

Papers by Falguni Modi

Veterinarski arhiv

Baicalein is a bioactive flavone originally isolated from the roots of Scutellaria baicalensis, S... more Baicalein is a bioactive flavone originally isolated from the roots of Scutellaria baicalensis, Scutellariala teriflora and Oroxylum indicum. The in vitro and in vivo anti-inflammatory and antibacterial properties of baicalein and pharmacokinetics after its single intramuscular administration were studied in Wistar rats. The in vitro anti-inflammatory activity of baicalein (10, 50 and 100 µM) was tested for its ability to inhibit the COX-2 enzyme by measuring PGE2 levels and determination of nitric oxide (NO) production in lipopolysaccharide (LPS) treated RAW 264.7 macrophage cells, in which baicalein was found to have significant inhibition of NO and PGE2 production in RAW 264.7 macrophage cells as compared with the LPS control group. The in vivo anti-inflammatory activity of baicalein (200 mg/kg) was assessed using the carrageenan-induced rat paw oedema model, following intramuscular injection. A significant percentage of inhibition of oedema volume was observed when compared with...

Journal of Phytonanotechnology and Pharmaceutical Sciences, Dec 1, 2021

The current study was carried out for evaluation of antibacterial activity of the endophytic fung... more The current study was carried out for evaluation of antibacterial activity of the endophytic fungi isolated from Bixa orellana L. plant leaves. Schizophyllum commune and Fusarium incarnatum were isolated and identified through molecular analysis of the internal transcribed region (ITS) of the ribosomal DNA from B. orellana. For large scale crude extraction of different endophytes grown on PDA was carried out and ethyl acetate was used for extraction. Antibacterial activity of ethyl acetate extract of endophyte Schizophyllum commune revealed MIC values against Staphylococcus aureus (0.16 µg/ml), Pseudomonas aerugonosa (2.56 µg/ml), Streptococcus pyogenus (5.12 µg/ml), Proteus mirabilis (0.08 µg/ml) and Bacillus subtillis (0.08 µg/ml), respectively and no effect was found against Escherichia coli and Salmonella typhimurium.

International Journal of Current Microbiology and Applied Sciences, Oct 10, 2018

Research Journal of Pharmacy and Technology, 2018

ISRN veterinary science, 2012

This study describes disposition of long-acting moxifloxacin and conventional formulations of mox... more This study describes disposition of long-acting moxifloxacin and conventional formulations of moxifloxacin in sheep after intravenous administration in five male sheep. Long acting moxifloxacin solution (10% moxifloxacin in solution with L-arginine, N-butyl alcohol, and benzyl alcohol) and conventional moxifloxacin (10%) were injected in jugular vein. Blood samples were collected from contralateral jugular vein in test tubes containing 30-50 IU heparin (anticoagulant) periodically from 0.083 to 72 h of drug administration. Drug concentrations in plasma were determined using High-Performance Liquid Chromatography (HPLC) with fluorescence detector. The mobile phase consisted of a mixture of buffer (10 gm of tetrabutyl ammonium hydrogen sulphate per liter-deionised water) and acetonitrile (80 : 20). The buffer was 0.067M of disodium hydrogen phosphate with pH of 7.5. The flow rate was 1 mL·min(-1) at ambient temperature. The effluent was monitored at 296 nm excitation and 504 nm emissi...

International Journal of Current Microbiology and Applied Sciences, Oct 10, 2018

International Journal of Current Microbiology and Applied Sciences, 2017

Annals of Phytomedicine: An International Journal, 2021

The current study was carried out to estimate pharmacokinetics of protocatechuic acid following o... more The current study was carried out to estimate pharmacokinetics of protocatechuic acid following oral administration of protocatechuic ethyl ester alone and in combination with piperine in rats. Following oral administration of protocatechuic ethyl ester, the mean peak plasma protocatechuic acid concentration of 1.50 ± 0.08 g/ml was achieved at 0.25 h. The pharmacokinetic parameters like mean absorption halflife (t ½) (0.16 ± 0.07), elimination half-life (t ½) (0.43 ± 0.02 h), apparent volume of distribution (Vdarea) (102.4 ± 5.76), area under plasma drug concentration-time curve (AUC 0-) (0.92 ± 0.02 µg h/ml), area under first moment curve (AUMC) (0.56 ± 0.01 µg h 2 /ml), total body clearance (Cl B) (163.7 ± 3.49 l/h/kg) and mean residence time (MRT) (0.61 ± 0.02) were observed for protocatechuic acid. Following oral administration of piperine with protocatechuic ethyl ester in rats, plasma concentrations of protocatechuic acid at all time point and pharmacokinetic parameters did not differ significantly in comparison to protocatechuic ethyl ester administered alone.

Annals of Phytomedicine: An International Journal, 2021

In vitro antibacterial effect of catechin was evaluated by determining MIC using microbroth dilut... more In vitro antibacterial effect of catechin was evaluated by determining MIC using microbroth dilution method against various gram-positive and negative typed culture and found MIC ranges from 1.25 to 10.0 mg/ml. In vivo antibacterial efficacy was measured in neutropenic rat thigh infection model and showed significant antibacterial effect. In vitro anti-inflammatory effect of catechin (10, 50 and 100 µM) was assessed by measuring COX-2 enzyme inhibition via detection of PGE2 concentration and NO production inhibition in LPS treated RAW 264.7 macrophage cells; whereas, in vivo anti-inflammatory efficacy of catechin was measured in carrageenan induced rat paw oedema model. Both in vitro and in vivo protocols revealed significant anti-inflammatory effect. Concentrations and pharmacokinetic profile of catechin were determined in rat plasma by high performance liquid chromatography (HPLC) after single intramuscular administration at dose of 200 mg/kg body weight. The mean peak plasma drug concentration of 16.36 ± 0.69 µg/ml was observed at 0.75 h. Pharmacokinetic parameters, viz., elimination half life (t ½), apparent volume of distribution (Vd (area)), total body clearance (Cl (B)) and mean residence time (MRT) were calculated as 1.79 ± 0.17 h, 18.62 ± 1.54 l/kg, 7.23 ± 0.15 l/h/kg and 1.78 ± 0.03 h, respectively. These results with pharmacokinetic profile suggest that catechin may be good candidate for antibacterial and antiinflammatory herbal formulation. Moreover, catechin may be used concurrent with available antibacterial and anti-inflammatory drugs to reduce resistance of microbes against drugs and side effects.

International Journal of Current Microbiology and Applied Sciences, 2020

The present study was planned to determine in vitro and in vivo antibacterial and antiinflammator... more The present study was planned to determine in vitro and in vivo antibacterial and antiinflammatory properties of Linalool. In vitro antibacterial activity of Linalool was determined by micro broth dilution technique and MICs were observed as1.25, 1.25, 1.25, 0.63, 1.25, 0.63 and 1.25 mg/ml against Staphylococcus aureus, Streptococcus pyogenus, Bacillus subtillis, Escherichia coli, Salmonella typhimurium, Pseudomonas aerugonosa and Proteus mirabilis, respectively. In vivo antibacterial efficacy of Linalool was determined in neutropenic rat thigh infection model and significant antibacterial efficacy was observed as compared to growth control with bacterial colony count 4.07 ± 0.05 Log 10 cfu/ml. In vitro anti-inflammatory activity was tested for their ability to inhibit COX-2 enzyme by measuring PGE 2 level and determination of NO production in LPS treated RAW264.7 macrophage cells. At all concentrations of Linalool (10, 50 and 100 µM) found to have significant inhibition of NO and PGE 2 production compared with LPS control group. In vivo anti-inflammatory activity of Linalool (100 mg/kg) was assessed using the carrageenan-induced rat paw edema model at different time intervals following intramuscular injection and observed significant percent inhibition of edema volume compared with carrageenan control group.

Indian Journal of Animal Health, 2018

A study was conducted on twenty (20) Surti buffaloes during their transient period categorized in... more A study was conducted on twenty (20) Surti buffaloes during their transient period categorized into two groups; treatment (n=10) and control (n=10) groups. In treatment group of animals to which 10 mL DL- tocopheryl acetate I.P. equivalent to tocopherol (vitamin E) base-50mg, sodium selenite U.S.P. equivalent to selenium base-1.5mg in each mL (E-CARE Se) and in control group animals 10 mL normal saline injected i/m on 60 th , 45 th , 30 th and 15 th day before expected date of parturition and after parturition on 15 th and 30 th day. The mean expulsion time of fetal membranes in the treatment group was found to be significantly shorter than that of control group. The mean placental weight in the treatment group was found to be non-significantly lower than that of control group. The calf weight in the treatment and control group did not differ significantly.

This study was conducted to evaluate the effect of calves sex on reproductive performance. A tota... more This study was conducted to evaluate the effect of calves sex on reproductive performance. A total 20 pregnant animals were selected. The expulsion time of foetal membranes, placental weight, calf weight, involution of uterus, first postpartum estrous, number of services per conception and service period of Surti buffaloes bearing male & female calves did not differ significantly.

The Indian Journal of Animal Reproduction, 2016

The cervical mucus and blood serum of normal fertile (n=10) and repeat breeder (n=20) Kankrej cat... more The cervical mucus and blood serum of normal fertile (n=10) and repeat breeder (n=20) Kankrej cattle revealed 8 to 10 protein bands by SDS-PAGE. The cervical mucus of repeat breeder cattle contained proteins of comparatively higher molecular weight than their normal fertile counterparts, whereas, the serum of normal fertile cattle contained proteins of higher molecular weight than the repeat breeder Kankrej cattle.

International Journal of Current Microbiology and Applied Sciences, 2018

Annals of Phytomedicine: An International Journal, 2019

The present study was undertaken to evaluate pharmacokinetics of ferulic acid following oral admi... more The present study was undertaken to evaluate pharmacokinetics of ferulic acid following oral administration of ethyl ferulate alone and in combination with piperine in rats. Following oral administration of ethyl ferulate and in combination with piperine, the mean peak plasma ferulic acid concentration of 18.38  1.38 vs 15.27  1.18 g/ ml was achieved at 0.25 h. Plasma concentration of ferulic acid at 0.5 h differ significantly (p <0.05) and plasma concentration of ferulic acid at 0.08 h, 0.25 h, 0.75 h and 1 h did not differ significantly. All pharmacokinetic parameter of ferulic acid did not differ significantly except volume of distribution (1.25 ± 0.12 vs 2.85 ± 0.57 L/ kg) and total body clearance (7.35 ± 0.57 vs 17.19 ± 1.59 L/h/kg).The study indicates rapid absorpti on and clearance of ferulic acid from body following oral administration of ethyl ferulate alone and in combination with piperine in rats.

Journal of Medicinal Chemistry, 2019

The identification of a novel class of potent pan-genotypic NS5A inhibitors with good pharmacokin... more The identification of a novel class of potent pan-genotypic NS5A inhibitors with good pharmacokinetic profile suitable for potential use in treating HCV infections is disclosed here. The present series of compounds are with less complex tricyclic central core, identified through a systematic SAR study carried out on biphenyl moiety. The SAR outcome has confirmed the requirement of near planar and linear conformation of the molecule to achieve the best pangenotypic activity. In addition, SAR with substituted imidazoles on improvement of antiviral activity is disclosed. The newly identified compounds 12, 16, 19−21 have shown desirable pharmacokinetic profiles with a favorable uptake of compounds in liver and maintained a significant concentration for up to 8 h in the liver. In addition, compounds 20 and 21 have shown superior pan-genotypic anti-HCV activity compared to ledipasvir and daclatasvir. Additional characterization and preliminary safety assessment resulted in the identification of compound 20 as a potential clinical candidate.

Annals of Phytomedicine: An International Journal, 2019

The stu dy was planned to evaluate pharmacokinetic profile and in vivo anti-inflammatory property... more The stu dy was planned to evaluate pharmacokinetic profile and in vivo anti-inflammatory property of rutin following intramuscular administration (100 mg/kg, intramuscular) in rats. Carrageenan-induced paw edema assay was carried out separately to study in vivo anti-inflammatory property of rutin. The plasma rutin concentration was assayed using High Performance Liquid Chromatography (HPLC). The pharmacokinetic parameters like the maximum plasma drug concentration (C max), time for maximal concentration (T max), elimination half-life (t ½), apparent volume of distribution (Vd (area)), total body clearance (Cl (B)) and mean residence time (MRT) were 21.11  0.46 g/ml, 1.83 ± 0.17 h, 9.11  1.50 h, 16.34  2.32 l/kg, 1.27  0.04 l/h/kg and 4.79  0.55 h, respectively. The drug concentration of 0.21  0.02 g/ml in plasma was detected at 24 h. In carrageenan-induced paw edema assay, rutin (100 mg/kg) significantly decreased edema volume from 1 to 6 h in comparison to carrageenan group and vehicle group. Per cent inhibition of inflammation after 6 h of rutin administration was 29.94 ± 1.49. Intramuscular administration of rutin produced satisfactory pharmacokinetic profile with promising in vivo anti-inflammatory activity in rats.

Scholars Journal of Agriculture and Veterinary Sciences, 2016

Reproductive problems in buffaloes are of significant economic concern in dairy farming. Maximizi... more Reproductive problems in buffaloes are of significant economic concern in dairy farming. Maximizing reproductive efficiency is the goal in dairy herds for better economics return. So, present work was conducted with an aim to know the incidence of periparturient problems usually found in buffaloes during the transition period. Out of total 99 adult head of Surti buffaloes on the farm, 79 calving were noted which revealed no incidence (0.00 %) of abortion, dystocia, retention of foetal membrane and postpartum genital prolapse, however, prepartum prolapse and still birth were observed in 2 (2.53 %) and 7 (8.86 %) animals, respectively, thus overall periparturient disorders was recorded as 11.39 per cent in Surti buffaloes.

Veterinary World, 2013

Aim: The study was carried out with an idea to ascertain involvement of trace minerals in failure... more Aim: The study was carried out with an idea to ascertain involvement of trace minerals in failure of conception in Knakrej cow. Materials and Methods: The animals under study were comprised of 10 normal (Group-I) and 20 repeat breeder (Group-II) Kankrej cows. Blood and estrual mucus of each repeat breeding and normally cycling cows were collected aseptically before breeding. The cervical mucus was diluted with triple glass distilled water at the rate of 1: 3 using vertex machine. Serum and diluted mucus was used for the trace minerals estimation. Results: The values observed for copper, iron, zinc and manganese in blood serum of normal and repeat breeding animals was 2.

International Journal for Agro Veterinary and Medical Sciences, 2011

A case of Dermal Melanocytoma in a Mehsana buffalo and its management has been reported.

Veterinarski arhiv

Baicalein is a bioactive flavone originally isolated from the roots of Scutellaria baicalensis, S... more Baicalein is a bioactive flavone originally isolated from the roots of Scutellaria baicalensis, Scutellariala teriflora and Oroxylum indicum. The in vitro and in vivo anti-inflammatory and antibacterial properties of baicalein and pharmacokinetics after its single intramuscular administration were studied in Wistar rats. The in vitro anti-inflammatory activity of baicalein (10, 50 and 100 µM) was tested for its ability to inhibit the COX-2 enzyme by measuring PGE2 levels and determination of nitric oxide (NO) production in lipopolysaccharide (LPS) treated RAW 264.7 macrophage cells, in which baicalein was found to have significant inhibition of NO and PGE2 production in RAW 264.7 macrophage cells as compared with the LPS control group. The in vivo anti-inflammatory activity of baicalein (200 mg/kg) was assessed using the carrageenan-induced rat paw oedema model, following intramuscular injection. A significant percentage of inhibition of oedema volume was observed when compared with...

Journal of Phytonanotechnology and Pharmaceutical Sciences, Dec 1, 2021

The current study was carried out for evaluation of antibacterial activity of the endophytic fung... more The current study was carried out for evaluation of antibacterial activity of the endophytic fungi isolated from Bixa orellana L. plant leaves. Schizophyllum commune and Fusarium incarnatum were isolated and identified through molecular analysis of the internal transcribed region (ITS) of the ribosomal DNA from B. orellana. For large scale crude extraction of different endophytes grown on PDA was carried out and ethyl acetate was used for extraction. Antibacterial activity of ethyl acetate extract of endophyte Schizophyllum commune revealed MIC values against Staphylococcus aureus (0.16 µg/ml), Pseudomonas aerugonosa (2.56 µg/ml), Streptococcus pyogenus (5.12 µg/ml), Proteus mirabilis (0.08 µg/ml) and Bacillus subtillis (0.08 µg/ml), respectively and no effect was found against Escherichia coli and Salmonella typhimurium.

International Journal of Current Microbiology and Applied Sciences, Oct 10, 2018

Research Journal of Pharmacy and Technology, 2018

ISRN veterinary science, 2012

This study describes disposition of long-acting moxifloxacin and conventional formulations of mox... more This study describes disposition of long-acting moxifloxacin and conventional formulations of moxifloxacin in sheep after intravenous administration in five male sheep. Long acting moxifloxacin solution (10% moxifloxacin in solution with L-arginine, N-butyl alcohol, and benzyl alcohol) and conventional moxifloxacin (10%) were injected in jugular vein. Blood samples were collected from contralateral jugular vein in test tubes containing 30-50 IU heparin (anticoagulant) periodically from 0.083 to 72 h of drug administration. Drug concentrations in plasma were determined using High-Performance Liquid Chromatography (HPLC) with fluorescence detector. The mobile phase consisted of a mixture of buffer (10 gm of tetrabutyl ammonium hydrogen sulphate per liter-deionised water) and acetonitrile (80 : 20). The buffer was 0.067M of disodium hydrogen phosphate with pH of 7.5. The flow rate was 1 mL·min(-1) at ambient temperature. The effluent was monitored at 296 nm excitation and 504 nm emissi...

International Journal of Current Microbiology and Applied Sciences, Oct 10, 2018

International Journal of Current Microbiology and Applied Sciences, 2017

Annals of Phytomedicine: An International Journal, 2021

The current study was carried out to estimate pharmacokinetics of protocatechuic acid following o... more The current study was carried out to estimate pharmacokinetics of protocatechuic acid following oral administration of protocatechuic ethyl ester alone and in combination with piperine in rats. Following oral administration of protocatechuic ethyl ester, the mean peak plasma protocatechuic acid concentration of 1.50 ± 0.08 g/ml was achieved at 0.25 h. The pharmacokinetic parameters like mean absorption halflife (t ½) (0.16 ± 0.07), elimination half-life (t ½) (0.43 ± 0.02 h), apparent volume of distribution (Vdarea) (102.4 ± 5.76), area under plasma drug concentration-time curve (AUC 0-) (0.92 ± 0.02 µg h/ml), area under first moment curve (AUMC) (0.56 ± 0.01 µg h 2 /ml), total body clearance (Cl B) (163.7 ± 3.49 l/h/kg) and mean residence time (MRT) (0.61 ± 0.02) were observed for protocatechuic acid. Following oral administration of piperine with protocatechuic ethyl ester in rats, plasma concentrations of protocatechuic acid at all time point and pharmacokinetic parameters did not differ significantly in comparison to protocatechuic ethyl ester administered alone.

Annals of Phytomedicine: An International Journal, 2021

In vitro antibacterial effect of catechin was evaluated by determining MIC using microbroth dilut... more In vitro antibacterial effect of catechin was evaluated by determining MIC using microbroth dilution method against various gram-positive and negative typed culture and found MIC ranges from 1.25 to 10.0 mg/ml. In vivo antibacterial efficacy was measured in neutropenic rat thigh infection model and showed significant antibacterial effect. In vitro anti-inflammatory effect of catechin (10, 50 and 100 µM) was assessed by measuring COX-2 enzyme inhibition via detection of PGE2 concentration and NO production inhibition in LPS treated RAW 264.7 macrophage cells; whereas, in vivo anti-inflammatory efficacy of catechin was measured in carrageenan induced rat paw oedema model. Both in vitro and in vivo protocols revealed significant anti-inflammatory effect. Concentrations and pharmacokinetic profile of catechin were determined in rat plasma by high performance liquid chromatography (HPLC) after single intramuscular administration at dose of 200 mg/kg body weight. The mean peak plasma drug concentration of 16.36 ± 0.69 µg/ml was observed at 0.75 h. Pharmacokinetic parameters, viz., elimination half life (t ½), apparent volume of distribution (Vd (area)), total body clearance (Cl (B)) and mean residence time (MRT) were calculated as 1.79 ± 0.17 h, 18.62 ± 1.54 l/kg, 7.23 ± 0.15 l/h/kg and 1.78 ± 0.03 h, respectively. These results with pharmacokinetic profile suggest that catechin may be good candidate for antibacterial and antiinflammatory herbal formulation. Moreover, catechin may be used concurrent with available antibacterial and anti-inflammatory drugs to reduce resistance of microbes against drugs and side effects.

International Journal of Current Microbiology and Applied Sciences, 2020

The present study was planned to determine in vitro and in vivo antibacterial and antiinflammator... more The present study was planned to determine in vitro and in vivo antibacterial and antiinflammatory properties of Linalool. In vitro antibacterial activity of Linalool was determined by micro broth dilution technique and MICs were observed as1.25, 1.25, 1.25, 0.63, 1.25, 0.63 and 1.25 mg/ml against Staphylococcus aureus, Streptococcus pyogenus, Bacillus subtillis, Escherichia coli, Salmonella typhimurium, Pseudomonas aerugonosa and Proteus mirabilis, respectively. In vivo antibacterial efficacy of Linalool was determined in neutropenic rat thigh infection model and significant antibacterial efficacy was observed as compared to growth control with bacterial colony count 4.07 ± 0.05 Log 10 cfu/ml. In vitro anti-inflammatory activity was tested for their ability to inhibit COX-2 enzyme by measuring PGE 2 level and determination of NO production in LPS treated RAW264.7 macrophage cells. At all concentrations of Linalool (10, 50 and 100 µM) found to have significant inhibition of NO and PGE 2 production compared with LPS control group. In vivo anti-inflammatory activity of Linalool (100 mg/kg) was assessed using the carrageenan-induced rat paw edema model at different time intervals following intramuscular injection and observed significant percent inhibition of edema volume compared with carrageenan control group.

Indian Journal of Animal Health, 2018

A study was conducted on twenty (20) Surti buffaloes during their transient period categorized in... more A study was conducted on twenty (20) Surti buffaloes during their transient period categorized into two groups; treatment (n=10) and control (n=10) groups. In treatment group of animals to which 10 mL DL- tocopheryl acetate I.P. equivalent to tocopherol (vitamin E) base-50mg, sodium selenite U.S.P. equivalent to selenium base-1.5mg in each mL (E-CARE Se) and in control group animals 10 mL normal saline injected i/m on 60 th , 45 th , 30 th and 15 th day before expected date of parturition and after parturition on 15 th and 30 th day. The mean expulsion time of fetal membranes in the treatment group was found to be significantly shorter than that of control group. The mean placental weight in the treatment group was found to be non-significantly lower than that of control group. The calf weight in the treatment and control group did not differ significantly.

This study was conducted to evaluate the effect of calves sex on reproductive performance. A tota... more This study was conducted to evaluate the effect of calves sex on reproductive performance. A total 20 pregnant animals were selected. The expulsion time of foetal membranes, placental weight, calf weight, involution of uterus, first postpartum estrous, number of services per conception and service period of Surti buffaloes bearing male & female calves did not differ significantly.

The Indian Journal of Animal Reproduction, 2016

The cervical mucus and blood serum of normal fertile (n=10) and repeat breeder (n=20) Kankrej cat... more The cervical mucus and blood serum of normal fertile (n=10) and repeat breeder (n=20) Kankrej cattle revealed 8 to 10 protein bands by SDS-PAGE. The cervical mucus of repeat breeder cattle contained proteins of comparatively higher molecular weight than their normal fertile counterparts, whereas, the serum of normal fertile cattle contained proteins of higher molecular weight than the repeat breeder Kankrej cattle.

International Journal of Current Microbiology and Applied Sciences, 2018

Annals of Phytomedicine: An International Journal, 2019

The present study was undertaken to evaluate pharmacokinetics of ferulic acid following oral admi... more The present study was undertaken to evaluate pharmacokinetics of ferulic acid following oral administration of ethyl ferulate alone and in combination with piperine in rats. Following oral administration of ethyl ferulate and in combination with piperine, the mean peak plasma ferulic acid concentration of 18.38  1.38 vs 15.27  1.18 g/ ml was achieved at 0.25 h. Plasma concentration of ferulic acid at 0.5 h differ significantly (p <0.05) and plasma concentration of ferulic acid at 0.08 h, 0.25 h, 0.75 h and 1 h did not differ significantly. All pharmacokinetic parameter of ferulic acid did not differ significantly except volume of distribution (1.25 ± 0.12 vs 2.85 ± 0.57 L/ kg) and total body clearance (7.35 ± 0.57 vs 17.19 ± 1.59 L/h/kg).The study indicates rapid absorpti on and clearance of ferulic acid from body following oral administration of ethyl ferulate alone and in combination with piperine in rats.

Journal of Medicinal Chemistry, 2019

The identification of a novel class of potent pan-genotypic NS5A inhibitors with good pharmacokin... more The identification of a novel class of potent pan-genotypic NS5A inhibitors with good pharmacokinetic profile suitable for potential use in treating HCV infections is disclosed here. The present series of compounds are with less complex tricyclic central core, identified through a systematic SAR study carried out on biphenyl moiety. The SAR outcome has confirmed the requirement of near planar and linear conformation of the molecule to achieve the best pangenotypic activity. In addition, SAR with substituted imidazoles on improvement of antiviral activity is disclosed. The newly identified compounds 12, 16, 19−21 have shown desirable pharmacokinetic profiles with a favorable uptake of compounds in liver and maintained a significant concentration for up to 8 h in the liver. In addition, compounds 20 and 21 have shown superior pan-genotypic anti-HCV activity compared to ledipasvir and daclatasvir. Additional characterization and preliminary safety assessment resulted in the identification of compound 20 as a potential clinical candidate.

Annals of Phytomedicine: An International Journal, 2019

The stu dy was planned to evaluate pharmacokinetic profile and in vivo anti-inflammatory property... more The stu dy was planned to evaluate pharmacokinetic profile and in vivo anti-inflammatory property of rutin following intramuscular administration (100 mg/kg, intramuscular) in rats. Carrageenan-induced paw edema assay was carried out separately to study in vivo anti-inflammatory property of rutin. The plasma rutin concentration was assayed using High Performance Liquid Chromatography (HPLC). The pharmacokinetic parameters like the maximum plasma drug concentration (C max), time for maximal concentration (T max), elimination half-life (t ½), apparent volume of distribution (Vd (area)), total body clearance (Cl (B)) and mean residence time (MRT) were 21.11  0.46 g/ml, 1.83 ± 0.17 h, 9.11  1.50 h, 16.34  2.32 l/kg, 1.27  0.04 l/h/kg and 4.79  0.55 h, respectively. The drug concentration of 0.21  0.02 g/ml in plasma was detected at 24 h. In carrageenan-induced paw edema assay, rutin (100 mg/kg) significantly decreased edema volume from 1 to 6 h in comparison to carrageenan group and vehicle group. Per cent inhibition of inflammation after 6 h of rutin administration was 29.94 ± 1.49. Intramuscular administration of rutin produced satisfactory pharmacokinetic profile with promising in vivo anti-inflammatory activity in rats.

Scholars Journal of Agriculture and Veterinary Sciences, 2016

Reproductive problems in buffaloes are of significant economic concern in dairy farming. Maximizi... more Reproductive problems in buffaloes are of significant economic concern in dairy farming. Maximizing reproductive efficiency is the goal in dairy herds for better economics return. So, present work was conducted with an aim to know the incidence of periparturient problems usually found in buffaloes during the transition period. Out of total 99 adult head of Surti buffaloes on the farm, 79 calving were noted which revealed no incidence (0.00 %) of abortion, dystocia, retention of foetal membrane and postpartum genital prolapse, however, prepartum prolapse and still birth were observed in 2 (2.53 %) and 7 (8.86 %) animals, respectively, thus overall periparturient disorders was recorded as 11.39 per cent in Surti buffaloes.

Veterinary World, 2013

Aim: The study was carried out with an idea to ascertain involvement of trace minerals in failure... more Aim: The study was carried out with an idea to ascertain involvement of trace minerals in failure of conception in Knakrej cow. Materials and Methods: The animals under study were comprised of 10 normal (Group-I) and 20 repeat breeder (Group-II) Kankrej cows. Blood and estrual mucus of each repeat breeding and normally cycling cows were collected aseptically before breeding. The cervical mucus was diluted with triple glass distilled water at the rate of 1: 3 using vertex machine. Serum and diluted mucus was used for the trace minerals estimation. Results: The values observed for copper, iron, zinc and manganese in blood serum of normal and repeat breeding animals was 2.

International Journal for Agro Veterinary and Medical Sciences, 2011

A case of Dermal Melanocytoma in a Mehsana buffalo and its management has been reported.