Fariborz Nowzari - Academia.edu (original) (raw)
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Papers by Fariborz Nowzari
Marine Drugs
This study set out to evaluate the wound healing properties of brittle star extracts in vitro and... more This study set out to evaluate the wound healing properties of brittle star extracts in vitro and in vivo. Due to the great arm regeneration potential of the brittle star, Ophiocoma cynthiae, the present study aimed to evaluate the wound healing effect of hydroalcoholic extracts of brittle star undergoing arm regeneration in wound healing models. The brittle star samples were collected from Nayband Bay, Bushehr, Iran. After wound induction in the arm of brittle stars, hydroalcoholic extracts relating to different times of arm regeneration were prepared. The GC-MS analysis, in vitro MTT cell viability and cell migration, Western blot, and computational analysis tests were performed. Based on the in vitro findings, two BSEs were chosen for in vivo testing. Macroscopic, histopathological and biochemical evaluations were performed after treatments. The results showed positive proliferative effects of BSEs. Specifically, forty-two compounds were detected in all groups of BSEs using GC-MS...
Veterinary Medicine and Science
Effects of cottonseed flour in male and female rats’ fertility based on hormonal and histomorphom... more Effects of cottonseed flour in male and female rats’ fertility based on hormonal and histomorphometry changes were studied. Sixty-four Sprague-Dawley adult male and female rats were randomly divided into control and treatment groups. Treatment group was received diets containing cottonseed flour for 35 days. Control group was given standard rat food. Body and testis weights, epididymis semen evaluation indices and serum sex steroid hormones were determined. Histomorphometry alterations of testes and ovary were evaluated. Then, normal female and male rats were mated by rats in both groups and after 35 days, number of pups was measured. However, there was no significant difference in whole body and testes weights, sperm concentration and viability between the control and treatment groups, respectively. Moreover, sperm motility in the treatment rats was significantly lower than the control group. Serum hormones alterations were not significant, but histomorphometry evaluations of teste...
Genetics Research
Polycystic ovary syndrome (PCOS) is a common endocrinopathy in women. PCOS is characterized by an... more Polycystic ovary syndrome (PCOS) is a common endocrinopathy in women. PCOS is characterized by anovulation, hyperandrogenism, polycystic ovaries, insulin resistance, and obesity. Despite the finding that the genetic origin of PCOS is well demonstrated in previous twin and familial clustering studies, genes and factors that can exactly explain the PCOS pathophysiology are not known. Objective(s). In this review, we attempted to identify genes related to secretion and signaling of insulin aspects of PCOS and their physiological functions in order to explain the pathways that are regulated by these genes which can be a prominent function in PCOS predisposition. Materials and Methods. For this purpose, published articles and reviews dealing with genetic evaluation of PCOS in women from peer-reviewed journals in PubMed and Google Scholar databases were included in this review. Results. The genomic investigations in women of different populations identified many candidate genes and loci t...
Free of Acrylamide Sodium Fast Free-of-Acrylamide Clearing Tissue (FACT) is a developed technique... more Free of Acrylamide Sodium Fast Free-of-Acrylamide Clearing Tissue (FACT) is a developed technique using no acrylamide for clearing tissues. As the lipid removal normally is a harmful process and it causes loss of biological molecules such as proteins and on the other hand is crucial for transparency and efficient antibody staining throughout the whole tissue especially for microscopy and imaging, the FACT technique is suitable since it makes chemical bonding of membrane and intracellular proteins with the extracellular matrix creating a massive three-dimensional (3D) matrix and structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, FACT requires less labor, toxic, and harmful chemicals. Here we describe protocols encompassing every angle and dimension of the FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving the structure and increasing the image quality. The entire protocol includes tissue pe...
Stem cells have an important role in regenerative therapies, developmental biology studies and dr... more Stem cells have an important role in regenerative therapies, developmental biology studies and drug screening. Basic and translational research in stem cell technology needs more detailed imaging techniques. The possibility of cell-based therapeutic strategies has been validated in the stem cell field over recent years, a more detailed characterization of the properties of stem cells is needed for connectomics of large assemblies and structural analyses of these cells. The aim of stem cell imaging is the characterization of differentiation state, cellular function, purity and cell location. Recent progress in stem cell imaging field has included ultrasound-based technique to study living stem cells and florescence microscopy-based technique to investigate stem cell three-dimensional (3D) structures. Here, we summarized the fundamental characteristics of stem cells via 3D imaging methods and also discussed the emerging literatures on 3D imaging in stem cell research and the applicati...
Marine Drugs
This study set out to evaluate the wound healing properties of brittle star extracts in vitro and... more This study set out to evaluate the wound healing properties of brittle star extracts in vitro and in vivo. Due to the great arm regeneration potential of the brittle star, Ophiocoma cynthiae, the present study aimed to evaluate the wound healing effect of hydroalcoholic extracts of brittle star undergoing arm regeneration in wound healing models. The brittle star samples were collected from Nayband Bay, Bushehr, Iran. After wound induction in the arm of brittle stars, hydroalcoholic extracts relating to different times of arm regeneration were prepared. The GC-MS analysis, in vitro MTT cell viability and cell migration, Western blot, and computational analysis tests were performed. Based on the in vitro findings, two BSEs were chosen for in vivo testing. Macroscopic, histopathological and biochemical evaluations were performed after treatments. The results showed positive proliferative effects of BSEs. Specifically, forty-two compounds were detected in all groups of BSEs using GC-MS...
Veterinary Medicine and Science
Effects of cottonseed flour in male and female rats’ fertility based on hormonal and histomorphom... more Effects of cottonseed flour in male and female rats’ fertility based on hormonal and histomorphometry changes were studied. Sixty-four Sprague-Dawley adult male and female rats were randomly divided into control and treatment groups. Treatment group was received diets containing cottonseed flour for 35 days. Control group was given standard rat food. Body and testis weights, epididymis semen evaluation indices and serum sex steroid hormones were determined. Histomorphometry alterations of testes and ovary were evaluated. Then, normal female and male rats were mated by rats in both groups and after 35 days, number of pups was measured. However, there was no significant difference in whole body and testes weights, sperm concentration and viability between the control and treatment groups, respectively. Moreover, sperm motility in the treatment rats was significantly lower than the control group. Serum hormones alterations were not significant, but histomorphometry evaluations of teste...
Genetics Research
Polycystic ovary syndrome (PCOS) is a common endocrinopathy in women. PCOS is characterized by an... more Polycystic ovary syndrome (PCOS) is a common endocrinopathy in women. PCOS is characterized by anovulation, hyperandrogenism, polycystic ovaries, insulin resistance, and obesity. Despite the finding that the genetic origin of PCOS is well demonstrated in previous twin and familial clustering studies, genes and factors that can exactly explain the PCOS pathophysiology are not known. Objective(s). In this review, we attempted to identify genes related to secretion and signaling of insulin aspects of PCOS and their physiological functions in order to explain the pathways that are regulated by these genes which can be a prominent function in PCOS predisposition. Materials and Methods. For this purpose, published articles and reviews dealing with genetic evaluation of PCOS in women from peer-reviewed journals in PubMed and Google Scholar databases were included in this review. Results. The genomic investigations in women of different populations identified many candidate genes and loci t...
Free of Acrylamide Sodium Fast Free-of-Acrylamide Clearing Tissue (FACT) is a developed technique... more Free of Acrylamide Sodium Fast Free-of-Acrylamide Clearing Tissue (FACT) is a developed technique using no acrylamide for clearing tissues. As the lipid removal normally is a harmful process and it causes loss of biological molecules such as proteins and on the other hand is crucial for transparency and efficient antibody staining throughout the whole tissue especially for microscopy and imaging, the FACT technique is suitable since it makes chemical bonding of membrane and intracellular proteins with the extracellular matrix creating a massive three-dimensional (3D) matrix and structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, FACT requires less labor, toxic, and harmful chemicals. Here we describe protocols encompassing every angle and dimension of the FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving the structure and increasing the image quality. The entire protocol includes tissue pe...
Stem cells have an important role in regenerative therapies, developmental biology studies and dr... more Stem cells have an important role in regenerative therapies, developmental biology studies and drug screening. Basic and translational research in stem cell technology needs more detailed imaging techniques. The possibility of cell-based therapeutic strategies has been validated in the stem cell field over recent years, a more detailed characterization of the properties of stem cells is needed for connectomics of large assemblies and structural analyses of these cells. The aim of stem cell imaging is the characterization of differentiation state, cellular function, purity and cell location. Recent progress in stem cell imaging field has included ultrasound-based technique to study living stem cells and florescence microscopy-based technique to investigate stem cell three-dimensional (3D) structures. Here, we summarized the fundamental characteristics of stem cells via 3D imaging methods and also discussed the emerging literatures on 3D imaging in stem cell research and the applicati...