Abdul Munim Farooq - Academia.edu (original) (raw)
Papers by Abdul Munim Farooq
Zea mays is an important cereal crop through out the world used as food for human and feed for li... more Zea mays is an important cereal crop through out the world used as food for human and feed for livestock. The crop yield is highly affected by biotic and abiotic stresses that include insects, pests, pathogens, weeds, drought, salinity, alkalinity, heat, cold etc. Among the biotic factors weeds caused major yield losses and affect plant growth due to nutrient, light, water, etc. competition with crop plants. The use of non-selective herbicides is highly un-safe because of the susceptibility of the corn plant to glyphosate herbicide. Therefore, there is need to develop such maize varieties and hybrids that have tolerance against glyphosate. The present review paper will contribute to understand the method of transformation of glyphosate gene in maize to develop glyphosate tolerant crop plants. [Qamar, Z, Aaliya K, Nasir IA, Farooq AM, Tabassum B, Ali Q, Awan MF, Ali A, Tariq M and Husnain T. An overview of genetic transformation of glyphosate resistant gene in Zea mays. Nat Sci 2015;...
Advancements in Life Sciences, 2019
Background: Transgenic technology reflects the incorporation of novel useful traits in crop plan... more Background: Transgenic technology reflects the incorporation of novel useful traits in crop plants like cotton for economic benefits by overcoming the problems including insects’ pests and weeds in special. The present study is the success story of the continuous effort of CEMB team started back in the 1990s. Methods: This study includes characterization of a large number of Bacillus thuringiensis (Bt) strains taken from local soil and subjected to direct transformation of isolated BT genes into local cotton cultivars. Protocols for transformation into cotton plants were optimized and validated by the development of double gene codon optimized (Cry1Ac and Cry2A) transgenic cotton varieties. Results: The resulting GMOs in the form of CEMB-33, CA-12, CEMB-66 have been approved by Punjab Seed Council in 2013 and 2016 respectively. Double Bt and weedicide resistant cotton harboring CEMB-Modified and codon optimized cp4EPSPS (GTGene). These varieties can tolerate glyphosate spray ...
In the present era the production and use of pesticides has been increased enormously and it has ... more In the present era the production and use of pesticides has been increased enormously and it has become the need to carry out such an assessment for the evaluation of pesticides effects on animals and mankind. For that, experiment has been performed to check the biosafety issue of pesticides by experimenting them on animals and also their toxicity has been analyzed on soil and plants. For this, maize crop was selected in the confined area. Pesticide used was Talstar that belongs to a pyrethroid group, and its active ingredient is bifenthrin. Plants were sprayed with this chemical and their toxicity was then checked on the animals. For such purpose four groups of chicken and two groups of rats were selected. Groups of chicken were divided as control, normal maize, high dose pesticide, and low dose pesticide whereas rats were divided as control and experimental group. They were subjected to the feeding trial of pesticide exposed feed for 45 days. Their weights were examined, and the h...
Journal of Biotechnology, 2021
Potato (Solanum tuberosum L.) is considered to be frost-susceptible as short spells of frost can ... more Potato (Solanum tuberosum L.) is considered to be frost-susceptible as short spells of frost can reduce the tuber yield and quality. Ice recrystallization inhibition (IRI) protein helps prevent growth of ice crystals in the cell apoplast during frost and help prevent damage associated with freezing stress. In this study, we investigated the in planta potential of Lolium perenne derived IRI3 transgene in improving the tolerance of transgenic potato lines for freezing stress. The codon optimized IRI3 transgene was introduced into potato cultivar Diamant through Agrobacterium mediated transformation. Three transgenic potato lines were successfully generated which were confirmed for transgene insertion and genomic integration by polymerase chain reaction and Southern blot. It was evident that the IRI3 transcript decreased in initial 24 h of cold stress treatment while the IRI3 mRNA expression up regulated in subsequent hours of cold treatment with maximum increase to 20 folds at 96 hrs post stress. A similar trend was also revealed in ion-leakage assay which showed that during cold stress, the transgenic potato lines depicted reduced ion leakage of 14 to 22% as compared to non-transgenic control plants. Further, the generated transgenic potato lines were tolerant to the frost spell in quarantine field conditions as compared to the non-transgenic potato lines. Additionally, the transgenic lines exhibited efficient recovery post frost injury in field conditions. The biochemical profiles of chlorophyll, proline and higher levels of antioxidant enzyme (superoxide dismutase, Catalase) activity and malondialdehyde levels showed that despite the phenotypic impact of low temperature, the transgenic potato lines quickly adjusted to maintain their cellular homeostasis post freezing stress by increasing the antioxidant defenses. This study suggests that up regulation of IRI3 transcript and regulatory network of cold stress response in transgenic potato lines improve frost tolerance and help stabilize yield in cultivated potato.
International Journal of Agriculture and Biology, 2017
In the evolution of geminiviruses, genetic recombination plays a key role in ecological, biochemi... more In the evolution of geminiviruses, genetic recombination plays a key role in ecological, biochemical and evolutionary processes. They evolve as results of prehistoric inter-genus recombination whereas intra-genus recombination of geminiviruses leads to the emergence of agriculturally important plant pathogens. Geminiviruses are transmitted by leafhoppers, treehoppers and whiteflies. An isolate of Chickpea chlorotic dwarf virus (CpCDV), a monopartite dicot infecting mastrevirus is transmitted by leafhopper. In contrast, Cotton leaf curl burewala virus (CLCuBuV), a monopartite begomovirus, was acquired and transmissible by whitefly to various host plants. In this study, we remove the coat protein (CP) gene of CpCDV by PCR and replaced with that of PCR amplified CLCuBuV-CP. The resultant infectious clone of chimeric CpCDV is produced later named mastrebegomo chimera (pGII0000MBC). By using Agroinfiltration technique (Agrobacterium-mediated inoculation) the chimeric clone along with wild type Clone CpCDV and CLCuBuV are injected on tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana benthamiana Domin.) seedlings and get symptoms after 21dpi (days post inoculation). Later, the symptoms appeared on plants were compared with healthy plants. This study elaborates our awareness about the genetic recombination and coexistence of mastreviruses with begomovirus globally including Pakistan and will provide the basic information regarding their management.
Pure and Applied Biology, 2019
Potato (Solanum tuberosum) is one of the largely consumed staple food crop of the world, includin... more Potato (Solanum tuberosum) is one of the largely consumed staple food crop of the world, including Pakistan. In Pakistan, potato shares a large percentage in country's agriculture GDP. In Pakistan majority of good quality virus free seed potato is imported by multinational and local seed companies. This costs a huge burden on country's economy. Potato Virus Y (PVY) belongs to Potyviridae family, severely damaging leaves of the Solanaceae family including potato. Plant lectins are the potential proteins that specifically bound and cross linked with carbohydrates and target glycans present on the cell surface of viruses through a pathway termed as lectin activation. The present in-vitro study shows the effect of lectin extracted from the seeds of Chenopodium murale against the downregulation of the CP (coat protein) gene of PVY. Stable interaction was found between PVY coat protein and CML insilico. Full length CP gene from PVY virus was amplified and cloned in mammalian expression vector. CML protein was purified to sub fractions through anion exchange chromatography on SP-sepharose gel. The non-cytotoxic effect and safe limit of CML extract was indicated by CC50 value. The hemagglutination activity of CML against the rabbit erythrocytes was revealed to be 35 µg/ml. The real-time PCR revealed the anti-PVY activity of CML extract. CML extract in concentration of 30, 60 and 90 µg/ml was found effective against PVY CP gene in comparison with control.
African Journal of Biotechnology, Oct 18, 2010
African Journal of Biotechnology Vol. 9(42), pp. 7026-7032, 18 October, 2010 Available online at ... more African Journal of Biotechnology Vol. 9(42), pp. 7026-7032, 18 October, 2010 Available online at http://www.academicjournals.org/AJB ISSN 16845315 ©2010 Academic Journals ... Viability assessment of in vitro produced synthetic ... Bushra Tabassum, Idrees Ahmad Nasir*, ...
AFRICAN JOURNAL OF AGRICULTURAL RESEEARCH, 2012
In this present study, six F1 hybrids (Maya, Roma, Rio Grande, Emotion F1 Hybrid, Tomato 55 and W... more In this present study, six F1 hybrids (Maya, Roma, Rio Grande, Emotion F1 Hybrid, Tomato 55 and Western Hybrid) were grown and their 15 crosses were evaluated. Out of 15 combination of crosses, 10 were recorded as successful viable hybrids, that is,
African Journal of Biotechnology, 2014
Planta, 1998
... trans-ferred for 10 d to P + 5Ao (instead of P + 5) supplemented with cefotaxime. ... compoun... more ... trans-ferred for 10 d to P + 5Ao (instead of P + 5) supplemented with cefotaxime. ... compounds in this phase of the experimental procedure; however, no positive effects were observed. ... Plant J 6: 271±282 Ho J, Vasil IK (1983) Somatic embryogenesis in sugarcane (Saccharum o ...
J. Agric. Res, 2010
Four tomato F1 hybrid cultivars (Roma, Maya, Rio Grande and T-55) were used to develop haploid li... more Four tomato F1 hybrid cultivars (Roma, Maya, Rio Grande and T-55) were used to develop haploid lines during 2005-08. Anthers were cytogenetically analyzed for anther size and meiotic stage. Anthers of varying sizes and meiotic stages were collected at different flowering ...
Viability assessment of in vitro produced synthetic seeds of cucumber
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2012
Potato (Solanum tuberosum L.) is one of the most economically important vegetable crops in Pakist... more Potato (Solanum tuberosum L.) is one of the most economically important vegetable crops in Pakistan. Chlorotic thickness veins spots intermingled with a dark green area, mosaic and decrease in size of the leaves were observed in the Lahore during a survey in 2009. Reverse transcriptase polymerase chain reaction (RT-PCR) based detection conditions were optimized for potato virus X using specific primers 5'-GGCGCAACTCCTGCCACAGC-3' and 5'-TTGTTGTTCCAGTGATACGA-3'. 613 bp amplicon of capsid protein (CP) gene was amplified, cloned and sequenced (Accession number HE577130). Comparisons as well as phylogenetic reconstructions of CP sequence with PVX sequences retrieved from Genebank showed that the Pakistani PVX isolates (HE577130) has close relationship with USSR isolate. This is the first report on the molecular characterization of full length PVX coat protein sequence infecting potato from Pakistan. Homology of the sequenced gene of PVX with reported genes in Gene Data Bank was observed within the range of 90 and 99.7%. Maximum homology was observed to be 99.7% with the gene (Genebank accession No. M38480 and M72416).
Zea mays is an important cereal crop through out the world used as food for human and feed for li... more Zea mays is an important cereal crop through out the world used as food for human and feed for livestock. The crop yield is highly affected by biotic and abiotic stresses that include insects, pests, pathogens, weeds, drought, salinity, alkalinity, heat, cold etc. Among the biotic factors weeds caused major yield losses and affect plant growth due to nutrient, light, water, etc. competition with crop plants. The use of non-selective herbicides is highly un-safe because of the susceptibility of the corn plant to glyphosate herbicide. Therefore, there is need to develop such maize varieties and hybrids that have tolerance against glyphosate. The present review paper will contribute to understand the method of transformation of glyphosate gene in maize to develop glyphosate tolerant crop plants. [Qamar, Z, Aaliya K, Nasir IA, Farooq AM, Tabassum B, Ali Q, Awan MF, Ali A, Tariq M and Husnain T. An overview of genetic transformation of glyphosate resistant gene in Zea mays. Nat Sci 2015;...
Advancements in Life Sciences, 2019
Background: Transgenic technology reflects the incorporation of novel useful traits in crop plan... more Background: Transgenic technology reflects the incorporation of novel useful traits in crop plants like cotton for economic benefits by overcoming the problems including insects’ pests and weeds in special. The present study is the success story of the continuous effort of CEMB team started back in the 1990s. Methods: This study includes characterization of a large number of Bacillus thuringiensis (Bt) strains taken from local soil and subjected to direct transformation of isolated BT genes into local cotton cultivars. Protocols for transformation into cotton plants were optimized and validated by the development of double gene codon optimized (Cry1Ac and Cry2A) transgenic cotton varieties. Results: The resulting GMOs in the form of CEMB-33, CA-12, CEMB-66 have been approved by Punjab Seed Council in 2013 and 2016 respectively. Double Bt and weedicide resistant cotton harboring CEMB-Modified and codon optimized cp4EPSPS (GTGene). These varieties can tolerate glyphosate spray ...
In the present era the production and use of pesticides has been increased enormously and it has ... more In the present era the production and use of pesticides has been increased enormously and it has become the need to carry out such an assessment for the evaluation of pesticides effects on animals and mankind. For that, experiment has been performed to check the biosafety issue of pesticides by experimenting them on animals and also their toxicity has been analyzed on soil and plants. For this, maize crop was selected in the confined area. Pesticide used was Talstar that belongs to a pyrethroid group, and its active ingredient is bifenthrin. Plants were sprayed with this chemical and their toxicity was then checked on the animals. For such purpose four groups of chicken and two groups of rats were selected. Groups of chicken were divided as control, normal maize, high dose pesticide, and low dose pesticide whereas rats were divided as control and experimental group. They were subjected to the feeding trial of pesticide exposed feed for 45 days. Their weights were examined, and the h...
Journal of Biotechnology, 2021
Potato (Solanum tuberosum L.) is considered to be frost-susceptible as short spells of frost can ... more Potato (Solanum tuberosum L.) is considered to be frost-susceptible as short spells of frost can reduce the tuber yield and quality. Ice recrystallization inhibition (IRI) protein helps prevent growth of ice crystals in the cell apoplast during frost and help prevent damage associated with freezing stress. In this study, we investigated the in planta potential of Lolium perenne derived IRI3 transgene in improving the tolerance of transgenic potato lines for freezing stress. The codon optimized IRI3 transgene was introduced into potato cultivar Diamant through Agrobacterium mediated transformation. Three transgenic potato lines were successfully generated which were confirmed for transgene insertion and genomic integration by polymerase chain reaction and Southern blot. It was evident that the IRI3 transcript decreased in initial 24 h of cold stress treatment while the IRI3 mRNA expression up regulated in subsequent hours of cold treatment with maximum increase to 20 folds at 96 hrs post stress. A similar trend was also revealed in ion-leakage assay which showed that during cold stress, the transgenic potato lines depicted reduced ion leakage of 14 to 22% as compared to non-transgenic control plants. Further, the generated transgenic potato lines were tolerant to the frost spell in quarantine field conditions as compared to the non-transgenic potato lines. Additionally, the transgenic lines exhibited efficient recovery post frost injury in field conditions. The biochemical profiles of chlorophyll, proline and higher levels of antioxidant enzyme (superoxide dismutase, Catalase) activity and malondialdehyde levels showed that despite the phenotypic impact of low temperature, the transgenic potato lines quickly adjusted to maintain their cellular homeostasis post freezing stress by increasing the antioxidant defenses. This study suggests that up regulation of IRI3 transcript and regulatory network of cold stress response in transgenic potato lines improve frost tolerance and help stabilize yield in cultivated potato.
International Journal of Agriculture and Biology, 2017
In the evolution of geminiviruses, genetic recombination plays a key role in ecological, biochemi... more In the evolution of geminiviruses, genetic recombination plays a key role in ecological, biochemical and evolutionary processes. They evolve as results of prehistoric inter-genus recombination whereas intra-genus recombination of geminiviruses leads to the emergence of agriculturally important plant pathogens. Geminiviruses are transmitted by leafhoppers, treehoppers and whiteflies. An isolate of Chickpea chlorotic dwarf virus (CpCDV), a monopartite dicot infecting mastrevirus is transmitted by leafhopper. In contrast, Cotton leaf curl burewala virus (CLCuBuV), a monopartite begomovirus, was acquired and transmissible by whitefly to various host plants. In this study, we remove the coat protein (CP) gene of CpCDV by PCR and replaced with that of PCR amplified CLCuBuV-CP. The resultant infectious clone of chimeric CpCDV is produced later named mastrebegomo chimera (pGII0000MBC). By using Agroinfiltration technique (Agrobacterium-mediated inoculation) the chimeric clone along with wild type Clone CpCDV and CLCuBuV are injected on tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana benthamiana Domin.) seedlings and get symptoms after 21dpi (days post inoculation). Later, the symptoms appeared on plants were compared with healthy plants. This study elaborates our awareness about the genetic recombination and coexistence of mastreviruses with begomovirus globally including Pakistan and will provide the basic information regarding their management.
Pure and Applied Biology, 2019
Potato (Solanum tuberosum) is one of the largely consumed staple food crop of the world, includin... more Potato (Solanum tuberosum) is one of the largely consumed staple food crop of the world, including Pakistan. In Pakistan, potato shares a large percentage in country's agriculture GDP. In Pakistan majority of good quality virus free seed potato is imported by multinational and local seed companies. This costs a huge burden on country's economy. Potato Virus Y (PVY) belongs to Potyviridae family, severely damaging leaves of the Solanaceae family including potato. Plant lectins are the potential proteins that specifically bound and cross linked with carbohydrates and target glycans present on the cell surface of viruses through a pathway termed as lectin activation. The present in-vitro study shows the effect of lectin extracted from the seeds of Chenopodium murale against the downregulation of the CP (coat protein) gene of PVY. Stable interaction was found between PVY coat protein and CML insilico. Full length CP gene from PVY virus was amplified and cloned in mammalian expression vector. CML protein was purified to sub fractions through anion exchange chromatography on SP-sepharose gel. The non-cytotoxic effect and safe limit of CML extract was indicated by CC50 value. The hemagglutination activity of CML against the rabbit erythrocytes was revealed to be 35 µg/ml. The real-time PCR revealed the anti-PVY activity of CML extract. CML extract in concentration of 30, 60 and 90 µg/ml was found effective against PVY CP gene in comparison with control.
African Journal of Biotechnology, Oct 18, 2010
African Journal of Biotechnology Vol. 9(42), pp. 7026-7032, 18 October, 2010 Available online at ... more African Journal of Biotechnology Vol. 9(42), pp. 7026-7032, 18 October, 2010 Available online at http://www.academicjournals.org/AJB ISSN 16845315 ©2010 Academic Journals ... Viability assessment of in vitro produced synthetic ... Bushra Tabassum, Idrees Ahmad Nasir*, ...
AFRICAN JOURNAL OF AGRICULTURAL RESEEARCH, 2012
In this present study, six F1 hybrids (Maya, Roma, Rio Grande, Emotion F1 Hybrid, Tomato 55 and W... more In this present study, six F1 hybrids (Maya, Roma, Rio Grande, Emotion F1 Hybrid, Tomato 55 and Western Hybrid) were grown and their 15 crosses were evaluated. Out of 15 combination of crosses, 10 were recorded as successful viable hybrids, that is,
African Journal of Biotechnology, 2014
Planta, 1998
... trans-ferred for 10 d to P + 5Ao (instead of P + 5) supplemented with cefotaxime. ... compoun... more ... trans-ferred for 10 d to P + 5Ao (instead of P + 5) supplemented with cefotaxime. ... compounds in this phase of the experimental procedure; however, no positive effects were observed. ... Plant J 6: 271±282 Ho J, Vasil IK (1983) Somatic embryogenesis in sugarcane (Saccharum o ...
J. Agric. Res, 2010
Four tomato F1 hybrid cultivars (Roma, Maya, Rio Grande and T-55) were used to develop haploid li... more Four tomato F1 hybrid cultivars (Roma, Maya, Rio Grande and T-55) were used to develop haploid lines during 2005-08. Anthers were cytogenetically analyzed for anther size and meiotic stage. Anthers of varying sizes and meiotic stages were collected at different flowering ...
Viability assessment of in vitro produced synthetic seeds of cucumber
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2012
Potato (Solanum tuberosum L.) is one of the most economically important vegetable crops in Pakist... more Potato (Solanum tuberosum L.) is one of the most economically important vegetable crops in Pakistan. Chlorotic thickness veins spots intermingled with a dark green area, mosaic and decrease in size of the leaves were observed in the Lahore during a survey in 2009. Reverse transcriptase polymerase chain reaction (RT-PCR) based detection conditions were optimized for potato virus X using specific primers 5'-GGCGCAACTCCTGCCACAGC-3' and 5'-TTGTTGTTCCAGTGATACGA-3'. 613 bp amplicon of capsid protein (CP) gene was amplified, cloned and sequenced (Accession number HE577130). Comparisons as well as phylogenetic reconstructions of CP sequence with PVX sequences retrieved from Genebank showed that the Pakistani PVX isolates (HE577130) has close relationship with USSR isolate. This is the first report on the molecular characterization of full length PVX coat protein sequence infecting potato from Pakistan. Homology of the sequenced gene of PVX with reported genes in Gene Data Bank was observed within the range of 90 and 99.7%. Maximum homology was observed to be 99.7% with the gene (Genebank accession No. M38480 and M72416).