Farshad Safari - Academia.edu (original) (raw)
Papers by Farshad Safari
Biotechnology progress, May 16, 2024
BioMed Research International
Background. Nowadays, due to various inherent properties, graphene-based nanoparticles are widely... more Background. Nowadays, due to various inherent properties, graphene-based nanoparticles are widely used in drug delivery research. On the other hand, folate receptors are highly expressed on the surface of human tumor cells. In this work, to enhance the 5-fluorouracil (5FU) and curcumin (Cur) effects on colon cancer, we constructed a folic acid- (FA-) modified codelivery carrier based on graphene nanoparticles (GO-Alb-Cur-FA-5FU). Materials and Methods. The HUVEC and HT-29 were selected for evaluating the antitumor effect of the prepared nanocarriers. The structure of nanocarriers was characterized by FTIR spectroscopy, X-ray diffraction analysis, TEM microscopy, and a DLS analyzer. The efficiency of the prepared carrier was evaluated by fluorescence microscopy using Annexin V and the PI kit. The cytotoxicity of the carrier’s component individually and the efficacy of the drug carrier GO-Alb-Cur-FA-5FU were assessed by MTT. Results. The results of the pharmacological tests indicated ...
Background Successful gene therapy relies on carriers to transfer genetic materials with high eff... more Background Successful gene therapy relies on carriers to transfer genetic materials with high efficiency and low toxicity in a targeted manner. To improve targeted cell binding and uptake, we developed and synthesized a new gene delivery vector based on graphene oxide (GO) modified by branched polyethyleneimine (BPEI) and folic acid (FA). Methods and Results The GO-PEI-FA nanocarriers exhibit less toxicity as compared to the unmodified PEI, as well as having potential efficacy in compressing and protecting pDNA. Interestingly, by increasing the polymer content in the polyplex formulation, the plasmid transfer ability increased. Graphene oxide substitution of PEI at N/P:10 on HepG2 cell line, improved hIL-12 expression by up to around eight folds relative to the simple PEI, which is 2-fold higher than Go-PEI-FA on Hek293 at the same N/P ratio. Conclusions Hence, the GO-PEI-FA described in this study might introduce as a targeting nanocarrier for delivery of hIL-12 plasmid into the ce...
![Research paper thumbnail of Interleukin-12 Plasmid DNA Delivery by N-[(2-Hydroxy-3-trimethylammonium)propyl]chitosan-Based Nanoparticles](https://attachments.academia-assets.com/89790231/thumbnails/1.jpg)
](Polymers
Cationic polysaccharides are capable of forming polyplexes with nucleic acids and are considered ... more Cationic polysaccharides are capable of forming polyplexes with nucleic acids and are considered promising polymeric gene carriers. The objective of this study was to evaluate the transfection efficiency and cytotoxicity of N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan salt (HTCS), a quaternary ammonium derivative of chitosan (CS), which benefits from non-ionizable positive charges. In this work, HTCS with a full quaternization of amino groups and a molar mass of 130,000 g·mol−1 was synthesized to use for delivery of a plasmid encoding the interleukin-12 (IL-12) gene. Thus, a polyplex based on HTCS and the IL-12 plasmid was prepared and then was characterized in terms of particle size, zeta potential, plasmid condensation ability, and protection of the plasmid against enzymatic degradation. We showed that HTCS was able to condense the IL-12 plasmid by the formation of polyplexes in the range of 74.5 ± 0.75 nm. The level of hIL-12 production following the transfection of the cel...
Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in ... more Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in different tissues. Glycine (Amino acetic acid) is the simplest amino acid incorporated in the structure of proteins. Several investigations indicate the hepatoprotective properties of these amino acids. On the other hand, antineoplastic agents-induced serum transaminase elevation and liver injury is a clinical complication. The current investigation was designed to screen the possible hepatoprotective properties of taurine and glycine against antineoplastic drugs-induced hepatic injury in an ex vivo model of isolated perfused rat liver. Rat liver was perfused with different concentration (10 µM, 100 µM and 1000 µM) of antineoplastic drugs (Mitoxantrone, Cyclophosphamide, Cisplatin, 5‑Fluorouracil, Doxorubicin and Dacarbazine) via portal vein. Taurine and glycine were administered to drug-treated livers and liver perfusate samples were collected for biochemical measurements (ALT, LDH, AST...
Background: Coronary artery disease (CAD) is considered to be one of the most pivotal causes of d... more Background: Coronary artery disease (CAD) is considered to be one of the most pivotal causes of death in the world. Over the past two decades, significant changes occurred in diagnosis, prognosis, and treatment of CAD, which has helped reduce mortality rates. miRNAs are a class of more than 5000 non-encoding RNA molecules (21 to 25 nucleotides across the length) that regulate the complex biological processes. Today, miRNAs are used to study cardiovascular diseases. In the present study, the expression of miR-146a،miR-27a ،miR-149 and miR-34a in plasma suffering from CAD and control group were investigated. Methods and Results: The present research was performed on 30 men with coronary artery stenosis (CAD) and 30 healthy men as controls. The expression levels of miR-146a, miR-27a, miR-149 and miR-34a in the plasma of patients with CAD and control group were measured using real-time PCR. Also, the correlation between the expression of circulating miRNAs levels and biochemical LDL-C, ...
Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in ... more Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in different tissues. Glycine (Amino acetic acid) is the simplest amino acid incorporated in the structure of proteins. Several investigations indicate the hepatoprotective properties of these amino acids. On the other hand, antineoplastic agents-induced serum transaminase elevation and liver injury is a clinical complication. The current investigation was designed to screen the possible hepatoprotective properties of taurine and glycine against antineoplastic drugs-induced hepatic injury in an ex vivo model of isolated perfused rat liver. Rat liver was perfused with different concentration (10 µM, 100 µM and 1000 µM) of antineoplastic drugs (Mitoxantrone, Cyclophosphamide, Cisplatin, 5‑Fluorouracil, Doxorubicin and Dacarbazine) via portal vein. Taurine and glycine were administered to drug-treated livers and liver perfusate samples were collected for biochemical measurements (ALT, LDH, AST...
Biotechnology progress, May 16, 2024
BioMed Research International
Background. Nowadays, due to various inherent properties, graphene-based nanoparticles are widely... more Background. Nowadays, due to various inherent properties, graphene-based nanoparticles are widely used in drug delivery research. On the other hand, folate receptors are highly expressed on the surface of human tumor cells. In this work, to enhance the 5-fluorouracil (5FU) and curcumin (Cur) effects on colon cancer, we constructed a folic acid- (FA-) modified codelivery carrier based on graphene nanoparticles (GO-Alb-Cur-FA-5FU). Materials and Methods. The HUVEC and HT-29 were selected for evaluating the antitumor effect of the prepared nanocarriers. The structure of nanocarriers was characterized by FTIR spectroscopy, X-ray diffraction analysis, TEM microscopy, and a DLS analyzer. The efficiency of the prepared carrier was evaluated by fluorescence microscopy using Annexin V and the PI kit. The cytotoxicity of the carrier’s component individually and the efficacy of the drug carrier GO-Alb-Cur-FA-5FU were assessed by MTT. Results. The results of the pharmacological tests indicated ...
Background Successful gene therapy relies on carriers to transfer genetic materials with high eff... more Background Successful gene therapy relies on carriers to transfer genetic materials with high efficiency and low toxicity in a targeted manner. To improve targeted cell binding and uptake, we developed and synthesized a new gene delivery vector based on graphene oxide (GO) modified by branched polyethyleneimine (BPEI) and folic acid (FA). Methods and Results The GO-PEI-FA nanocarriers exhibit less toxicity as compared to the unmodified PEI, as well as having potential efficacy in compressing and protecting pDNA. Interestingly, by increasing the polymer content in the polyplex formulation, the plasmid transfer ability increased. Graphene oxide substitution of PEI at N/P:10 on HepG2 cell line, improved hIL-12 expression by up to around eight folds relative to the simple PEI, which is 2-fold higher than Go-PEI-FA on Hek293 at the same N/P ratio. Conclusions Hence, the GO-PEI-FA described in this study might introduce as a targeting nanocarrier for delivery of hIL-12 plasmid into the ce...
Polymers
Cationic polysaccharides are capable of forming polyplexes with nucleic acids and are considered ... more Cationic polysaccharides are capable of forming polyplexes with nucleic acids and are considered promising polymeric gene carriers. The objective of this study was to evaluate the transfection efficiency and cytotoxicity of N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan salt (HTCS), a quaternary ammonium derivative of chitosan (CS), which benefits from non-ionizable positive charges. In this work, HTCS with a full quaternization of amino groups and a molar mass of 130,000 g·mol−1 was synthesized to use for delivery of a plasmid encoding the interleukin-12 (IL-12) gene. Thus, a polyplex based on HTCS and the IL-12 plasmid was prepared and then was characterized in terms of particle size, zeta potential, plasmid condensation ability, and protection of the plasmid against enzymatic degradation. We showed that HTCS was able to condense the IL-12 plasmid by the formation of polyplexes in the range of 74.5 ± 0.75 nm. The level of hIL-12 production following the transfection of the cel...
Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in ... more Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in different tissues. Glycine (Amino acetic acid) is the simplest amino acid incorporated in the structure of proteins. Several investigations indicate the hepatoprotective properties of these amino acids. On the other hand, antineoplastic agents-induced serum transaminase elevation and liver injury is a clinical complication. The current investigation was designed to screen the possible hepatoprotective properties of taurine and glycine against antineoplastic drugs-induced hepatic injury in an ex vivo model of isolated perfused rat liver. Rat liver was perfused with different concentration (10 µM, 100 µM and 1000 µM) of antineoplastic drugs (Mitoxantrone, Cyclophosphamide, Cisplatin, 5‑Fluorouracil, Doxorubicin and Dacarbazine) via portal vein. Taurine and glycine were administered to drug-treated livers and liver perfusate samples were collected for biochemical measurements (ALT, LDH, AST...
Background: Coronary artery disease (CAD) is considered to be one of the most pivotal causes of d... more Background: Coronary artery disease (CAD) is considered to be one of the most pivotal causes of death in the world. Over the past two decades, significant changes occurred in diagnosis, prognosis, and treatment of CAD, which has helped reduce mortality rates. miRNAs are a class of more than 5000 non-encoding RNA molecules (21 to 25 nucleotides across the length) that regulate the complex biological processes. Today, miRNAs are used to study cardiovascular diseases. In the present study, the expression of miR-146a،miR-27a ،miR-149 and miR-34a in plasma suffering from CAD and control group were investigated. Methods and Results: The present research was performed on 30 men with coronary artery stenosis (CAD) and 30 healthy men as controls. The expression levels of miR-146a, miR-27a, miR-149 and miR-34a in the plasma of patients with CAD and control group were measured using real-time PCR. Also, the correlation between the expression of circulating miRNAs levels and biochemical LDL-C, ...
Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in ... more Taurine (2-aminoethane sulfonic acid) is a non-protein amino acid found in high concentration in different tissues. Glycine (Amino acetic acid) is the simplest amino acid incorporated in the structure of proteins. Several investigations indicate the hepatoprotective properties of these amino acids. On the other hand, antineoplastic agents-induced serum transaminase elevation and liver injury is a clinical complication. The current investigation was designed to screen the possible hepatoprotective properties of taurine and glycine against antineoplastic drugs-induced hepatic injury in an ex vivo model of isolated perfused rat liver. Rat liver was perfused with different concentration (10 µM, 100 µM and 1000 µM) of antineoplastic drugs (Mitoxantrone, Cyclophosphamide, Cisplatin, 5‑Fluorouracil, Doxorubicin and Dacarbazine) via portal vein. Taurine and glycine were administered to drug-treated livers and liver perfusate samples were collected for biochemical measurements (ALT, LDH, AST...