Farzaneh Sadat Motafeghi - Academia.edu (original) (raw)

Papers by Farzaneh Sadat Motafeghi

Toxicology Research, Dec 31, 2023

Inorganic Chemistry Communications, Dec 1, 2022

Neurotoxicity Research, Dec 16, 2022

مجله مطالعات علوم پزشکی, Jun 1, 2022

Background & Aims: In today's society, chemicals have many benefits and applications. One of thes... more Background & Aims: In today's society, chemicals have many benefits and applications. One of these chemicals is Drabkin's solution which is used in the hematology laboratory. Drabkin's solution is toxic and is harmful to skin health. In this study, considering the chemical composition of vitamin C and the phytochemical properties of ginger plant, we decided to investigate the effects of the hydroalcoholic solution of this plant on the survival of normal skin and gum cells in the presence of Drabkin solution. Materials & Methods: Protective effects of ginger extract and vitamin C (doses 0.1, 10, 50, 100, 500, and 1000 μM) on the toxicity caused by Drabkin's solution was measured by colorimetric assay (MTT) to investigate the survival and viability of normal gingival and skin cells. Prism statistical software version 8 and one-way ANOVA and Tukey tests were used for statistical analysis. A significance level of P<0.05 was considered. Results: The results of this study showed that high concentrations of vitamin C and ginger inhibit the toxicity of Drabkin's solution, so that there was a significant increase in the growth of normalized cells. Drabkin's solution in concentrations higher than 50 μM has toxicity on the studied cell lines. Conclusion: The results of this study showed that the consumption of ginger extract and vitamin C in different concentrations increased the growth of normal cells of the gums and skin, and as a result, caused cell protection from the damage caused by Drabkin's solution.

Pharmaceutical and biomedical research, Mar 7, 2023

Background::Cancer is one of the problems facing societies today and despite new advances in chem... more Background::Cancer is one of the problems facing societies today and despite new advances in chemotherapy and cancer treatment, there are still many cancers that do not respond to today's treatments. Tarragon with the scientific name of Artemisia dracunculus L. has various flavonoid and polyphenolic compounds and many therapeutic effects. Objectives: This study aimed to investigate the cytotoxicity of this plant on different categories of cancer cels. Methodes: After collecting the shoots of tarragon and extracting them by the maceration method, the weight of the extract with a yield of 22.25% was 12.9 g. After examining the presence of flavonoids and total phenol, the extract's antioxidant activity was examined using DPPH and FRAP methods. Finally, MTT tests on three cancer cell lines, MCF-7, HT-29, and MKN45, were done using different concentrations of tarragon extract (100, 200, 500, and 1000 μg/mL). Results: Total flavonoids were detected at 24±1.18 mg of quercetin per gram of extract, and total phenols were detected at 59±2.21 mg of gallic acid per gram of extract. Examining the inhibitory effect of DPPH compared to vitamin C, it was found that the hydroalcoholic extract of tarragon has a 50% inhibitory effect. According to the standard curve, the amount of iron reduced by tarragon hydroalcoholic extract is equal to 405±0.11 μg/ml. The cytotoxic effect of tarragon hydroalcoholic extract on MCF7, MKN45, and HT-29 cell lines was investigated, and their IC50 values were 1065.669, 881.19, and 743.870 μg/mL, respectively. The A. dracunculus L extract inhibits the growth of cancer cells in various cell lines. Conclusion: According to antioxidant tests, it can be said that the anti-cancer effects of tarragon are based on its antioxidant power and phenolic and flavonoid compounds.

Applied in vitro toxicology, Mar 1, 2023

Toxicology Mechanisms and Methods, Aug 8, 2022

Drug and Chemical Toxicology, May 16, 2022

Journal of Biomimetics, Biomaterials and Biomedical Engineering, May 31, 2023

طب پیشگیری طبری, Aug 22, 2021

Introduction: Various studies have shown that vincristine and permethrin have genetic toxicity on... more Introduction: Various studies have shown that vincristine and permethrin have genetic toxicity on the body's normal cells. Due to the widespread use of these drugs, preventing their toxicity is essential; Therefore, in this study, the protective effects of vitamin C and melatonin on the genetic toxicity induced by vincristine and permethrin in peripheral blood lymphocytes were investigated. Material and Methods: The protective effects of vitamin C and melatonin (doses of 50, 100, and 200 µm) on the toxicity of vincristine and permethrin induced by micronucleus test on peripheral blood lymphocytes were evaluated, and in statistical tests P <0.05 as the significant level was considered. Results: According to the results, vincristine and permethrin caused genetic disorder by 28.80±1.92 and 34±1.58 micronucleus, respectively (p<0.0001). However, by exposing vitamin c and melatonin with permethrin at concentrations of 100 μM and 200 μM, the number of micronuclei was significantly decreased by 24.80±2.91, 18.00±1.58 (Vit C) and 22.20±3.34, 15.40±1.14 (melatonin) respectively. In comparison, exposure of these two substances with vincristine in similar concentrations reduced the micronucleus by 16.60±2.07, 10.80±0.83 (Vit C) and 13.00±1.58, 6.40±1.14 (melatonin), respectively. Conclusion: As the results of this study showed, permethrin and vincristine both caused genetic toxicity. Melatonin can protect against DNA damage by purifying reactive oxygen species or stimulating the DNA repair system. Vitamin C plays an essential protective role in many toxic reactions of the body. Both antioxidants have been shown to reduce the genetic toxicity of permethrin and vincristine.

Toxicology in Vitro, Mar 1, 2023

Social Science Research Network, 2022

DOAJ (DOAJ: Directory of Open Access Journals), 2023

A new kind of nanocomposite based on silver nanoparticles (AgNPs)/graphene oxide (GO) was conveni... more A new kind of nanocomposite based on silver nanoparticles (AgNPs)/graphene oxide (GO) was conveniently achieved through a green and low-cost synthesis approach using glucose as a reducing and stabilizing agent, and the synthetic procedure can be easily used for the construction of a disposable electrochemical sensor on glassy carbon electrode (GCE). The nanocomposite was detailedly characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). The 2 experimental results demonstrated that the nanocomposite possessed the specific features of both silver nanoparticles and graphene, and the intrinsic high specific area and the fast electron transfer rate ascribed to the nanohybrid structure could improve its electrocatalytic performance greatly. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed to evaluate the electrochemical properties of AgNPs/GO/GCE towards tryptophan, and the AgNPs/GO film exhibited a distinctly higher activity for the electro-oxidation of tryptophan than GO film with tenfold enhancement of peak current. The oxidation mechanism and the kinetic parameters were investigated, and analysis operation conditions were optimized. Under the selected experimental conditions, the oxidation peak currents were proportional to tryptophan concentrations over the range of 0.01 μM to 50.0 μM and 50.0 μM to 800.0 μM, respectively. The detection limit was 2.0 nM (S/N = 3). Moreover, the proposed method is free of interference from tyrosine and other coexisting species. The resulting sensor displays excellent repeatability and long-term stability; finally it was successfully applied to detect tryptophan in real samples with good recoveries, ranging from 99.0% to 103.0%.

مجله مطالعات علوم پزشکی, Apr 1, 2023

Background & Aims: The aim of this study was to investigate, the protective effects of ginger ext... more Background & Aims: The aim of this study was to investigate, the protective effects of ginger extract and acetylcysteine were investigated on genotoxicity caused by sodium azide in peripheral blood lymphocytes. Sodium azide is known as a powerful genetic mutagen in various organisms including bacteria, plants, and animals, and is considered a genotoxic agent that widely affects many organisms. Materials & Methods: In this experimental study, the hydroalcoholic extract of ginger (0.1, 0.5, and 1 µM) and acetylcysteine (50, 100, and 500 µM) were tested for their protective effects on genotoxicity caused by sodium azide in lymphocytes. The micronucleus method was used to analyze human blood samples. Data collected from the experiment were analyzed using Graph Pad Prism v8 statistical software, with P<0.05 considered as a significant level. Results: The results showed that sodium azide induces genotoxicity in human blood lymphocytes, causing the formation of micronuclei. Treatment of lymphocytes with different concentrations of acetylcysteine and ginger reduced the production of micronuclei in a dose-dependent manner, leading to a reduction in genotoxicity (p<0.05). Conclusion: The study concluded that N-acetyl-cysteine, at concentrations of 100 and 500 µM, and ginger, at all doses, led to a dosedependent reduction in genotoxicity. This suggests that N-acetyl-cysteine and compounds found in the ginger extract have high antioxidant power, enabling them to reduce the genetic toxicity caused by sodium azide.

Pharmaceutical and biomedical research, Oct 10, 2021

Background: Cadmium is a heavy metal that can cause various injuries in the body, including nephr... more Background: Cadmium is a heavy metal that can cause various injuries in the body, including nephrotoxicity. L-Arginine is a metal chelator that can prevent oxidative damage caused by oxygen free radicals. Objectives: This study aimed to investigate the effect of L-arginine in inhibiting mitochondrial toxicity induced by subchronic cadmium exposure in the kidney of male mice. Methods: A total of 42 male mice were randomly divided into six groups (n=6): control (normal saline), cadmium (2 mg/kg), cadmium (2 mg/kg) plus three doses of L-arginine (50, 100, and 200 mg/kg) and finally cadmium (2 mg/kg) plus vitamin C (500 mg/kg). After 42 days, the animals were anesthetized with ketamine/xylazine. Their kidney tissues were removed, and mitochondrial fractions were isolated. Oxidative stress factors and mitochondrial damage parameters (MTT, swelling, and mitochondrial membrane potential) were measured in renal isolated mitochondria. Also, evaluation of Blood Urea Nitrogen (BUN) and Creatinine (Cr) tests were done. Results: Significant rise in BUN and Cr were observed in cadmium-treated mice (P<0.05). Cadmium enhanced oxidative stress in the kidney via increasing lipid peroxidation and oxidation of protein and glutathione. It caused significant mitochondrial dysfunction, mitochondrial membrane potential collapse, and swelling in isolated mitochondria (P<0.05). L-Arginine significantly ameliorated cadmium-induced oxidative stress and mitochondrial damage (P<0.05). Furthermore, a significant reduction in serum BUN and Cr were observed in L-arginine received group (P<0.05). Conclusion: The results showed that L-arginine has significant protective effects against cadmium-induced renal toxicity in male mice.

European Journal of Pharmaceutical Sciences, Nov 1, 2021

In continuation of our research to find strong and safe anticonvulsant agents, a number of (aryla... more In continuation of our research to find strong and safe anticonvulsant agents, a number of (arylalkyl)azoles (AAAs) containing naphthylthiazole and naphthyloxazole scaffolds were designed and synthesized. The in vivo anticonvulsant evaluations in BALB/c mice revealed that some of them had significant anticonvulsant activity in both maximal electroshock (MES) and pentylenetetrazole (PTZ) models of epilepsy. The best profile of activity was observed with compounds containing imidazole and triazole rings (C1, C6, G1, and G6). In particular, imidazolylmethyl-thiazole C1 with median effective dose (ED50)= 7.9 mg/kg in the MES test, ED50= 27.9 mg/kg in PTZ test, and without any sign of neurotoxicity (in the rotarod test, 100 mg/kg) was the most promising compound. The patch-clamp recording was performed to study the mechanism of action of the representative compound C1 on hippocampal dentate gyrus (DG) cells. The results did not confirm any modulatory effect of C1 on the voltage-gated ion channels (VGICs) or GABAa agonism, but suggested a significant reduction of excitatory postsynaptic currents (EPSCs) frequency on hippocampal DG neurons. Sub-acute toxicity studies revealed that administration of the most active compounds (C1, C6, G1, and G6) at 100 mg/kg bw/day for two weeks did not result in any mortality or significant toxicity as evaluated by assessment of biochemical markers such as lipid peroxidation, intracellular glutathione, total antioxidant capacity, histopathological changes, and mitochondrial functions. Other pharmacological aspects of compounds including mechanistic and ADME properties were investigated computationally and/or experimentally. Molecular docking on the NMDA and AMPA targets suggested that the introduction of the heterocyclic ring in the middle of AAAs significantly affects the affinity of the compounds. The obtained results totally demonstrated that the prototype compound C1 can be considered as a new lead for the development of anticonvulsant agents.

Applied In Vitro Toxicology

Toxicology Research

Metformin exerts its anticancer effect through two mechanisms, directly affecting the tumor and... more Metformin exerts its anticancer effect through two mechanisms, directly affecting the tumor and indirectly reducing systemic insulin levels. The anticancer effects of aspirin occur by inhibiting Cyclooxygenase (COX)-2. COX-2 is absent in many cell types under normal conditions and increases under pathological conditions such as cancer. This study aims to investigate the effect of metformin and aspirin and their combination of them on A549 and PC3 cell lines. Metformin and aspirin were investigated separately and in combination on two cancer cell lines, A549 and PC3. The examined groups include the negative control of untreated cells and the positive control of cisplatin and drugs at concentrations of 15, 10, and 20 μg/ mL to investigate the mechanism of oxidative stress factors (reactive oxygen species, lipid peroxidation, Glutathione (GSH)) and apoptosis (lactate dehydrogenase). The results showed that aspirin, metformin, and their combination could affect cancer cell growth by d...

Studies in Medical Sciences

Pharmaceutical and Biomedical Research