Fasihi Ramandi - Academia.edu (original) (raw)
Papers by Fasihi Ramandi
Ssu_Journals, Sep 15, 2010
Ssu_Journals, Jun 15, 2014
BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important ro... more BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important role in physiology of myocardial adaptation to ischemia. In cardiomyocytes, the pore-forming subunits of these channels are coded by KCNJ11 gene. It was reported that ...
Artificial Cells, Nanomedicine, and Biotechnology, 2016
Introduction The early secreted antigenic target 6-kDa protein (ESAT-6) plays an important role i... more Introduction The early secreted antigenic target 6-kDa protein (ESAT-6) plays an important role in immune protection against Tuberculosis. Owing to its great potential to increase immune response, chitosan can be considered as a suitable biodegradable polymer for intranasal administration. Methods The physiochemical properties of the nanoparticle were measured in vitro. Two weeks after the last intranasal administration, blood samples were collected and specific IgG, IFN-gama, and IL-4 levels were measured by ELISA. Results Chitosan nanoparticles containing ESAT-6 demonstrated stronger ability to induce IFN-gama, IL-4, and IgG antibody level than the control groups. Conclusion Administration of chitosan nanoparticles can be a suitable method to induce more appropriate immune responses against low inherent immunogenic tuberculosis proteins through intranasal routs.
Iranian biomedical journal, Jan 2, 2016
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an infectious zoonotic pathogen causing huma... more Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an infectious zoonotic pathogen causing human infections. These infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. The first intimate bacterial adhesion, intimin (I), with its own receptor translocated intimin receptor (Tir) and E. coli secreted protein A, acting as Tir conduit, are highly immunogenic proteins for vaccine development against E. coli O157:H7. A chimeric trivalent recombinant protein was previously found to be a suitable strategy for developing vaccines against E. coli O157:H7. In this study, the recombinant EIT (rEIT) was used to design a protective EHEC nasal nanovaccine. Chitosan and its water-soluble derivative, trimethylated chitosan (TMC), as muco-adhesive biopolymers, are good candidates for preparation of nanovaccines. Using the electrospraying technique, as a novel method, we could obtain particles of rEIT loaded with chitosan an...
Veterinary Research Communications, 2015
Brucellosis is the most common zoonotic bacterial disease. Prevention of human brucellosis is ach... more Brucellosis is the most common zoonotic bacterial disease. Prevention of human brucellosis is achieved through pasteurization of dairy products, appropriate sanitation and vaccination of domestic animals against the Brucella species. B. abortus unlipidated 19 kDa outer membrane protein (U-Omp19) is a promising candidate for a subunit vaccine against brucellosis. This study investigates immunogenicity of Omp19 alone and with Freund's adjuvant (Omp19-IFA) and Ntrimethyl chitosan (TMC/Omp19) nanoparticles, as well as the effect of Omp19 administration route on immunological responses and protection. The omp19 gene was expressed in E. coli BL21 (DE3). After purification, the recombinant Omp19 was loaded onto TMC nanoparticles by ionic gelation with tripolyphosphate. Particle size and loading efficiency of the nanoparticles were determined. Omp19-IFA was administered intraperitoneally while TMC/Omp19 nanoparticles were administered orally and intraperitoneally. The results indicated that intraperitoneal (i.p.) immunization by Omp19-IFA and TMC/Omp19 nanoparticles induced Th1 and Th2 immune responses, respectively, whereas oral immunization of TMC/ Omp19 nanoparticles induced a mixed Th1/Th17 immune response. Moreover, oral immunization increased IgA levels in feces. Immunized mice were challenged with virulent B. melitensis 16 M and B. abortus 544. Oral immunization with TMC/Omp19 nanoparticles induced a remarkably high protection level against B. melitensis and B. abortus. The results showed that immunization route has a pivotal role in immune response polarization and protective efficiency of Omp19 antigen. Also, it was deduced that the higher protection level achieved through oral administration of TMC/Omp19 nanoparticles may be due to the elicited Th17 response.
Iranian Journal of Biotechnology, 2014
Asia-Pacific psychiatry : official journal of the Pacific Rim College of Psychiatrists, Jan 23, 2015
Serotonin transporter gene linked polymorphic region, also called 5HTTLPR, is a candidate in the ... more Serotonin transporter gene linked polymorphic region, also called 5HTTLPR, is a candidate in the genetics of bipolar disorder; however, the results of previous association studies are inconsistent. Several explanations have been proposed for that inconsistency; among them are the existing differences both in the genetic basis of bipolar disorder subtypes and the genetic backgrounds of the studied populations. We aimed to investigate the association of 5HTTLPR with bipolar disorder type I (BP-1) in Iranian population. In this case-control study, 146 patients with BP-1 and 165 controls were recruited. The patients were selected through the Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders, 4th edition. It was required that the patients do not have any present history of general medical conditions, substance abuse, and concurrent major psychiatric disorders. The polymorphism was evaluated by blood sampling and subsequent DNA extraction, polymerase ...
Journal of Drug Delivery Science and Technology, 2014
Nasal Shigellosis vaccine is quite attractive for inducing a protective immune response. The reas... more Nasal Shigellosis vaccine is quite attractive for inducing a protective immune response. The reason is the invading effect of Shigella spp. to body through mucosal surfaces. Chitosan is a mucoadhesive polysaccharide with great potential for nasal vaccine delivery regarding to the exclusive features including biocompatibility, biodegradability, high charge density and non-toxicity. In this study, the potential of chitosan nanofibrous membrane as a novel carrier system for the nasal delivery of shigella subunit vaccine was investigated. The antigen-containing chitosan nanofibrous membrane was prepared by electrospinning of chitosan/AcOH solution. Guinea pigs were vaccinated by direct administration of antigen-containing chitosan nanofibers into the nasal cavity. The guinea pigs immunized intranasal administered N-IpaD/NFs showed higher serum and mucosal antibody responses comparing to those of other groups. All immunized animals were subsequently protected against a challenge with wild-type S. flexneri 2a in a keratoconjunctivitis Sereny test that N-IpaD/NFs group was protective.
Laboratory Medicine, 2011
Laboratory Medicine, 2011
Koomesh, 2010
ﺪﻤﺤﻣ ﻜﺸﻣ ﻦﺴﺣ ﻲ فﺎﺑ 1 (Ph.D) ، ﻬﻠﻟاﺪﺒﻋ سﺎﺒﻋ ﻲ 2 (M.Sc)* ، ﺪﻬﻣ ي ﺼﻓ ﻴﺤ ﻲ ﺪﻨﻣار ي 3 (Ph.D) ،ﺳ ﻴﺪ ﺮﻔﻌ... more ﺪﻤﺤﻣ ﻜﺸﻣ ﻦﺴﺣ ﻲ فﺎﺑ 1 (Ph.D) ، ﻬﻠﻟاﺪﺒﻋ سﺎﺒﻋ ﻲ 2 (M.Sc)* ، ﺪﻬﻣ ي ﺼﻓ ﻴﺤ ﻲ ﺪﻨﻣار ي 3 (Ph.D) ،ﺳ ﻴﺪ ﺮﻔﻌﺟ ﻧﺎﻧﺪﻋ ﻲ ﺗادﺎﺳ ﻲ 2 ) M.Sc ( ، ... ﻠﻋ ﻲ جوﺮﻣ 2 ) M.Sc (، ﺷﻴ اﻮ ﻤﺗﺎﺣ ﻲ 2 ) B.Sc ( ... 1-ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﺎﺴﻓ ، هوﺮﮔ ﺑﻴ ﺷﻮﻴ ﻤﻲ ... 2-ﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲ ﺴﻓ ﮔ ،ﺎ ﻣ هوﺮ ﻴ بوﺮﻜ ﺳﺎﻨﺷ ﻲ ... 3-ﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲ ﻘﺑﻴﻪ ﷲا ) ﺞﻋ ( ، هوﺮﮔ ﺑﻴ ...
ARYA …, 2010
BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important ro... more BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important role in physiology of myocardial adaptation to ischemia. In cardiomyocytes, the pore-forming subunits of these channels are coded by KCNJ11 gene. It was reported that ...
World Journal of Microbiology and Biotechnology, 2014
Ssu_Journals, Sep 15, 2010
Ssu_Journals, Jun 15, 2014
BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important ro... more BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important role in physiology of myocardial adaptation to ischemia. In cardiomyocytes, the pore-forming subunits of these channels are coded by KCNJ11 gene. It was reported that ...
Artificial Cells, Nanomedicine, and Biotechnology, 2016
Introduction The early secreted antigenic target 6-kDa protein (ESAT-6) plays an important role i... more Introduction The early secreted antigenic target 6-kDa protein (ESAT-6) plays an important role in immune protection against Tuberculosis. Owing to its great potential to increase immune response, chitosan can be considered as a suitable biodegradable polymer for intranasal administration. Methods The physiochemical properties of the nanoparticle were measured in vitro. Two weeks after the last intranasal administration, blood samples were collected and specific IgG, IFN-gama, and IL-4 levels were measured by ELISA. Results Chitosan nanoparticles containing ESAT-6 demonstrated stronger ability to induce IFN-gama, IL-4, and IgG antibody level than the control groups. Conclusion Administration of chitosan nanoparticles can be a suitable method to induce more appropriate immune responses against low inherent immunogenic tuberculosis proteins through intranasal routs.
Iranian biomedical journal, Jan 2, 2016
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an infectious zoonotic pathogen causing huma... more Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an infectious zoonotic pathogen causing human infections. These infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. The first intimate bacterial adhesion, intimin (I), with its own receptor translocated intimin receptor (Tir) and E. coli secreted protein A, acting as Tir conduit, are highly immunogenic proteins for vaccine development against E. coli O157:H7. A chimeric trivalent recombinant protein was previously found to be a suitable strategy for developing vaccines against E. coli O157:H7. In this study, the recombinant EIT (rEIT) was used to design a protective EHEC nasal nanovaccine. Chitosan and its water-soluble derivative, trimethylated chitosan (TMC), as muco-adhesive biopolymers, are good candidates for preparation of nanovaccines. Using the electrospraying technique, as a novel method, we could obtain particles of rEIT loaded with chitosan an...
Veterinary Research Communications, 2015
Brucellosis is the most common zoonotic bacterial disease. Prevention of human brucellosis is ach... more Brucellosis is the most common zoonotic bacterial disease. Prevention of human brucellosis is achieved through pasteurization of dairy products, appropriate sanitation and vaccination of domestic animals against the Brucella species. B. abortus unlipidated 19 kDa outer membrane protein (U-Omp19) is a promising candidate for a subunit vaccine against brucellosis. This study investigates immunogenicity of Omp19 alone and with Freund's adjuvant (Omp19-IFA) and Ntrimethyl chitosan (TMC/Omp19) nanoparticles, as well as the effect of Omp19 administration route on immunological responses and protection. The omp19 gene was expressed in E. coli BL21 (DE3). After purification, the recombinant Omp19 was loaded onto TMC nanoparticles by ionic gelation with tripolyphosphate. Particle size and loading efficiency of the nanoparticles were determined. Omp19-IFA was administered intraperitoneally while TMC/Omp19 nanoparticles were administered orally and intraperitoneally. The results indicated that intraperitoneal (i.p.) immunization by Omp19-IFA and TMC/Omp19 nanoparticles induced Th1 and Th2 immune responses, respectively, whereas oral immunization of TMC/ Omp19 nanoparticles induced a mixed Th1/Th17 immune response. Moreover, oral immunization increased IgA levels in feces. Immunized mice were challenged with virulent B. melitensis 16 M and B. abortus 544. Oral immunization with TMC/Omp19 nanoparticles induced a remarkably high protection level against B. melitensis and B. abortus. The results showed that immunization route has a pivotal role in immune response polarization and protective efficiency of Omp19 antigen. Also, it was deduced that the higher protection level achieved through oral administration of TMC/Omp19 nanoparticles may be due to the elicited Th17 response.
Iranian Journal of Biotechnology, 2014
Asia-Pacific psychiatry : official journal of the Pacific Rim College of Psychiatrists, Jan 23, 2015
Serotonin transporter gene linked polymorphic region, also called 5HTTLPR, is a candidate in the ... more Serotonin transporter gene linked polymorphic region, also called 5HTTLPR, is a candidate in the genetics of bipolar disorder; however, the results of previous association studies are inconsistent. Several explanations have been proposed for that inconsistency; among them are the existing differences both in the genetic basis of bipolar disorder subtypes and the genetic backgrounds of the studied populations. We aimed to investigate the association of 5HTTLPR with bipolar disorder type I (BP-1) in Iranian population. In this case-control study, 146 patients with BP-1 and 165 controls were recruited. The patients were selected through the Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders, 4th edition. It was required that the patients do not have any present history of general medical conditions, substance abuse, and concurrent major psychiatric disorders. The polymorphism was evaluated by blood sampling and subsequent DNA extraction, polymerase ...
Journal of Drug Delivery Science and Technology, 2014
Nasal Shigellosis vaccine is quite attractive for inducing a protective immune response. The reas... more Nasal Shigellosis vaccine is quite attractive for inducing a protective immune response. The reason is the invading effect of Shigella spp. to body through mucosal surfaces. Chitosan is a mucoadhesive polysaccharide with great potential for nasal vaccine delivery regarding to the exclusive features including biocompatibility, biodegradability, high charge density and non-toxicity. In this study, the potential of chitosan nanofibrous membrane as a novel carrier system for the nasal delivery of shigella subunit vaccine was investigated. The antigen-containing chitosan nanofibrous membrane was prepared by electrospinning of chitosan/AcOH solution. Guinea pigs were vaccinated by direct administration of antigen-containing chitosan nanofibers into the nasal cavity. The guinea pigs immunized intranasal administered N-IpaD/NFs showed higher serum and mucosal antibody responses comparing to those of other groups. All immunized animals were subsequently protected against a challenge with wild-type S. flexneri 2a in a keratoconjunctivitis Sereny test that N-IpaD/NFs group was protective.
Laboratory Medicine, 2011
Laboratory Medicine, 2011
Koomesh, 2010
ﺪﻤﺤﻣ ﻜﺸﻣ ﻦﺴﺣ ﻲ فﺎﺑ 1 (Ph.D) ، ﻬﻠﻟاﺪﺒﻋ سﺎﺒﻋ ﻲ 2 (M.Sc)* ، ﺪﻬﻣ ي ﺼﻓ ﻴﺤ ﻲ ﺪﻨﻣار ي 3 (Ph.D) ،ﺳ ﻴﺪ ﺮﻔﻌ... more ﺪﻤﺤﻣ ﻜﺸﻣ ﻦﺴﺣ ﻲ فﺎﺑ 1 (Ph.D) ، ﻬﻠﻟاﺪﺒﻋ سﺎﺒﻋ ﻲ 2 (M.Sc)* ، ﺪﻬﻣ ي ﺼﻓ ﻴﺤ ﻲ ﺪﻨﻣار ي 3 (Ph.D) ،ﺳ ﻴﺪ ﺮﻔﻌﺟ ﻧﺎﻧﺪﻋ ﻲ ﺗادﺎﺳ ﻲ 2 ) M.Sc ( ، ... ﻠﻋ ﻲ جوﺮﻣ 2 ) M.Sc (، ﺷﻴ اﻮ ﻤﺗﺎﺣ ﻲ 2 ) B.Sc ( ... 1-ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﺎﺴﻓ ، هوﺮﮔ ﺑﻴ ﺷﻮﻴ ﻤﻲ ... 2-ﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲ ﺴﻓ ﮔ ،ﺎ ﻣ هوﺮ ﻴ بوﺮﻜ ﺳﺎﻨﺷ ﻲ ... 3-ﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲ ﻘﺑﻴﻪ ﷲا ) ﺞﻋ ( ، هوﺮﮔ ﺑﻴ ...
ARYA …, 2010
BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important ro... more BACKGROUND: It has been shown that ATP-sensitive potassium (K ATP ) channels play an important role in physiology of myocardial adaptation to ischemia. In cardiomyocytes, the pore-forming subunits of these channels are coded by KCNJ11 gene. It was reported that ...
World Journal of Microbiology and Biotechnology, 2014