Fatemeh Mirzaee - Academia.edu (original) (raw)

Papers by Fatemeh Mirzaee

Food Science and Nutrition, Jul 4, 2023

PubMed, Feb 1, 2018

Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species ... more Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species distributed among the world. Based on the studies of Persian medicine texts, there are some promising bioactivities for this genus that is unknown in modern medicine and some of them are still the basis of new remedies. This article presents Arnebia according to the most important ancient information by the most famous Persian medicine books like Makhzan Al Advieh, Tohfat Al-Momenin, Al-Qanun, Al-Seidaneh and Ekhtiarate Badiei. A search of electronic databases including PubMed, Scopus, Science Direct, and Google Scholar was done to find articles published between 1991 and 2017 on pharmacology and phytochemistry of Arnebia spp. In Persian medicine texts, Arnebia's different exclusive forms of preparations are effective for treatment of some disorders such as diarrhoea, amenorrhea, gout, kidney stone, jaundice, chronic fever and burn wounds. There are some activities that are the same in Persian and modern medicine research such as burn wound healing and fever amelioration. Phytochemical investigations on the title genus have led to characterization of many secondary metabolites. Naphthoquinones such as alkannins, shikonins, and their derivatives are the major constituents that have shown pharmacological activities in different Arnebia species. Among the major properties of Arnebia, only two of them (burn healing and fever amelioration) were investigated in modern medicine. The major aforementioned properties discussed in details in ancient sources might be a novel research sources leading to important discoveries in clinical usages of Arnebia.

South African Journal of Botany, May 1, 2021

Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with rema... more Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with remarkable protective activities against various chemical carcinogenesis and toxicities. Protective role of the hydroalcoholic extract and desulfo glucosinolate fraction from seeds of Lepidium perfoliatum were evaluated against CCl4-induced hepatotoxicity in BALB/c mice by determination of oxidative stress biomarkers and liver histopathological feature. The oral administration of different doses of the extract and desulfo glucosinolate fraction exhibited significant decrease in ROS and MDA contents of mitochondria in a dose-dependent manner, especially the doses of 200 mg/kg of the extract and 10 mg/kg of the fraction. GSH content was significantly increased (p

Turkish journal of pharmaceutical sciences, Feb 1, 2023

Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of disea... more Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of diseases in humans. Cytotoxicity and emergence of new strains resistance to the current anti-leishmanial drugs have encouraged researchers to focus on new resources. Glucosinolates (GSL) are found mainly in the Brassicaceae family with potential cytotoxic and anti-parasitic properties. The present study reports in vitro antileishmanial activity of the GSL fraction from Alyssum linifolium seeds against Leishmania major. Materials and Methods: The GSL fraction was prepared by ion-exchange and reversed-phase chromatography. For the assessment of antileishmanial activity, the promastigotes and amastigotes of L. major were treated with different concentrations of the fraction (75-625 μg/mL). Results: The IC 50 was 245 μg/mL for anti-promastigote effect of the GSL fraction and 250 μg/mL for its anti-amastigote effect that had a significant difference (p<0.05) with both glucantime and amphotericin B. The selectivity index of the GSL fraction (15.8), to glucantime and amphotericin B, was greater than 10, indicating the selective effect of this fraction against L. major amastigotes. Glucoiberverin was the major constituent of the GSL fraction characterized using nuclear magnetic resonance and electron ionization-mass spectrometry spectra. Based on gas chromatographymass spectrometry data, iberverin and iberverin nitrile, the hydrolysis constituents from glucoiberverin, included 76.91% of the total seed volatiles. Conclusion: The results suggest that GSLs like glucoiberverin could be considered a new promising candidate for further studies on antileishmanial activity.

DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2019

Background and objectives: Traditionally, Geum species from Rosaceae family have been used for tr... more Background and objectives: Traditionally, Geum species from Rosaceae family have been used for treating inflammatory disorders. Geum iranicum Khatamsaz is endemic to Iran. The aim of this study was to investigate the antinociceptive and anti-inflammatory activities of Geum iranicum methanol extract G. iranicum methanol extract using classical models. Methods: The methanol extract of G. iranicum roots was evaluated for antinociceptive activity by acetic acid-induced writhing, formalin and hot-plate tests in male Swiss albino mice. The anti-inflammatory effect was investigated by Carrageenan-induced paw edema method. Results: The extract significantly inhibited both the first and second phases of formalin-induced nociception in mice at the dose of 100 mg/kg compared to the control group. In acetic acid-induced writhing test and hot plate method, the extract significantly reduced pain behavior in all doses (25, 50 and 100 mg/kg). The antinociceptive activity of the extract was significantly reduced by naloxone (4 mg/kg). The anti-inflammatory activity of the extract was found to be dose dependent. The extract at the dose of 100 mg/kg exhibited significant reduction of paw edema in all surveyed times. Conclusion: The results showed that the methanol extract of G. iranicum roots possessed central analgesic activity via modulation of opioid receptors as well as antiinflammatory activity. The observed effects could be attributed to the presence of constituents like triterpenoids, eugenol, sucrose and tannins in the extract.

Food Science & Nutrition

South African Journal of Botany

Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with rema... more Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with remarkable protective activities against various chemical carcinogenesis and toxicities. Protective role of the hydroalcoholic extract and desulfo glucosinolate fraction from seeds of Lepidium perfoliatum were evaluated against CCl4-induced hepatotoxicity in BALB/c mice by determination of oxidative stress biomarkers and liver histopathological feature. The oral administration of different doses of the extract and desulfo glucosinolate fraction exhibited significant decrease in ROS and MDA contents of mitochondria in a dose-dependent manner, especially the doses of 200 mg/kg of the extract and 10 mg/kg of the fraction. GSH content was significantly increased (p

Turkish Journal of Pharmaceutical Sciences

Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of disea... more Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of diseases in humans. Cytotoxicity and emergence of new strains resistance to the current anti-leishmanial drugs have encouraged researchers to focus on new resources. Glucosinolates (GSL) are found mainly in the Brassicaceae family with potential cytotoxic and anti-parasitic properties. The present study reports in vitro antileishmanial activity of the GSL fraction from Alyssum linifolium seeds against Leishmania major. Materials and Methods: The GSL fraction was prepared by ion-exchange and reversed-phase chromatography. For the assessment of antileishmanial activity, the promastigotes and amastigotes of L. major were treated with different concentrations of the fraction (75-625 μg/mL). Results: The IC 50 was 245 μg/mL for anti-promastigote effect of the GSL fraction and 250 μg/mL for its anti-amastigote effect that had a significant difference (p<0.05) with both glucantime and amphotericin B. The selectivity index of the GSL fraction (15.8), to glucantime and amphotericin B, was greater than 10, indicating the selective effect of this fraction against L. major amastigotes. Glucoiberverin was the major constituent of the GSL fraction characterized using nuclear magnetic resonance and electron ionization-mass spectrometry spectra. Based on gas chromatographymass spectrometry data, iberverin and iberverin nitrile, the hydrolysis constituents from glucoiberverin, included 76.91% of the total seed volatiles. Conclusion: The results suggest that GSLs like glucoiberverin could be considered a new promising candidate for further studies on antileishmanial activity.

Research Journal of Pharmacognosy, 2018

Jaundice is recognized by increased bilirubin level of blood and yellow appearance in skin, scler... more Jaundice is recognized by increased bilirubin level of blood and yellow appearance in skin, sclera and mucous membrane. Natural products have been used for treatment of jaundice for a long time and Persian Medicine can be a good source of natural drugs for this purpose. This study was done to find the materia medica used for jaundice based on Persian medicine (PM) texts including The Canon of Medicine (al-qanun), Al Hawi Fi Al Tibb, Tuhfat al-Mu’minin, Hedayat al-Motealemin fi Tibb, Zakhireye Khwarazmshahi, Al-Abniah 'an Haqaeq al-Adwia, Makhzan ul-Adwia and Ikhtiyarat Badie. One hundred eleven materia medica belonging to 51 families were identified as herbal remedies for treating jaundice. Apiaceae, Asteraceae, Cucurbitaceae, Fabaceae, Lamiaceae, Rosaceae and Zingiberaceae were the most dominant families. The results of this study may be present sources for pharmacological studies and development of new herbal medicines for jaundice. Asparagus, echium, arnebia, chicory, citron ...

Annals of parasitology, 2020

Leishmaniosis is one of the most serious public health concern with a worldwide distribution. Sin... more Leishmaniosis is one of the most serious public health concern with a worldwide distribution. Since the current treatments of leishmaniosis are toxic and expensive, frequent studies have been conducted to investigate the benefits of new resources such as medicinal plants for treatment of this infectious disease. Recent studies revealed the antiparasitic potential of Rhus coriaria. Here we investigated the potential antileishmanial and antibacterial activities of the hydroalcoholic extract of R. coriaria fruits. The fruits were extracted using 80% methanol by maceration method. The concentrations of 312, 156, 78, and 37 μg/ml of the extract were added separately to the wells containing Leishmania major (L. major) promastigotes and amastigotes. Amphotericin B was considered as positive control. Finally, the death rate was determined for the extract-treated parasites as compared to the non-treated parasite. The antibacterial activity was evaluated by measurement of the minimum inhibito...

Medicinski glasnik : official publication of the Medical Association of Zenica-Doboj Canton, Bosnia and Herzegovina, 2018

Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species ... more Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species distributed among the world. Based on the studies of Persian medicine texts, there are some promising bioactivities for this genus that is unknown in modern medicine and some of them are still the basis of new remedies. This article presents Arnebia according to the most important ancient information by the most famous Persian medicine books like Makhzan Al Advieh, Tohfat Al-Momenin, Al-Qanun, Al-Seidaneh and Ekhtiarate Badiei. A search of electronic databases including PubMed, Scopus, Science Direct, and Google Scholar was done to find articles published between 1991 and 2017 on pharmacology and phytochemistry of Arnebia spp. In Persian medicine texts, Arnebia's different exclusive forms of preparations are effective for treatment of some disorders such as diarrhoea, amenorrhea, gout, kidney stone, jaundice, chronic fever and burn wounds. There are some activities that are the same ...

Journal of Mazandaran University of Medical Sciences, 2021

Background and purpose: There are few treatment options available for treatment of toxoplasmosis ... more Background and purpose: There are few treatment options available for treatment of toxoplasmosis and effective drugs have serious toxic effects. In this study, the in vivo and in vitro anti-toxoplasma activities of Heracleum persicum and Foeniculum vulgare fruits essential oils were investigated. Materials and methods: In vitro, Vero cells were incubated with different concentrations of essential oils or pyrimethamine (positive control) and the cellular viability was determined. Next, Vero cells were infected with T. gondii (RH strain) and treated with agents. Then, the CC50, IC50, and selectivity index (SI) were calculated. Moreover, in vivo, the effect of oils on survival times of Balb/c mice infected with T. gondii were determined. Results: In vitro results showed that the oils exhibited less cell toxicity than pyrimethamine. The selective index was 2.94, 6.96, and 3.06 for Heracleum persicum, Foeniculum vulgare, and pyrimethamine, respectively. Also, the infected mice treated wi...

Research Journal of Pharmacognosy, 2020

Background and objectives: Colchicum kurdicum (Bornm.) Stef. is a flowering perennial monocotyled... more Background and objectives: Colchicum kurdicum (Bornm.) Stef. is a flowering perennial monocotyledon plant that has many important bioactive compounds especially colchicine and colchicine derivatives. In this study, the ultrasound-assisted acidic-solvent extraction method coupled with response-surface method was presented as the successful method for large scale extraction of colchicine as an alkaloid compound from Colchicum kurdicum. Methods: According to the literatures, methanol/deionized water (70:30) solvent system was selected for the extraction. In addition, the response-surface method was used for analysis and optimization of colchicine extraction by ultrasonic-assisted acidic-solvent extraction method. Subsequently, colchicine was extracted using this method and the effects of solvent pH, extraction time, solvent/plant ratio, power, and temperature were evaluated. Results: After all analysis procedures, 0.99 mg colchicine/g dried corms was achieved with the following conditi...

Research Journal of Pharmacognosy, 2019

Physalis, the fifth largest genus of the Solanaceae family, consists many species which are used ... more Physalis, the fifth largest genus of the Solanaceae family, consists many species which are used as food and natural remedies. Phytochemical investigations on Physalis species have led to characterization of important secondary metabolites such as withaphysalins, physalins and phytosterols which are responsible for wide range of pharmacological effects such as potent cytotoxic activity against various type of human cancer cell lines, immunomodulatory and cancer chemopreventive effects. Theyare widely used in the indigenous system of medicine for treatment of kidney and urinary disorders, leishmaniasis, inflammations, skin infections and etc. Based on Iranian traditional medicine texts, Physalis is effective for treatment of several disorders such as asthma, liver, kidney and bladder dysfunctions, wounds, hemorrhoids, and helminthic diseases. Based on recent surveys, many of the above properties have not been investigated so far except the for the healing effects on liver dysfunction...

Research Journal of Pharmacognosy, 2019

Background and objectives:Traditionally, Geum species from Rosaceae family have been used for tre... more Background and objectives:Traditionally, Geum species from Rosaceae family have been used for treating inflammatory disorders. Geum iranicum Khatamsaz is endemic to Iran. The aim of this study was to investigate the antinociceptive and anti-inflammatory activities of Geum iranicum methanol extract G. iranicum methanol extract using classical models. Methods: The methanol extract of G. iranicum roots was evaluated for antinociceptive activity by acetic acid-induced writhing, formalin and hot-plate tests in male Swiss albino mice. The anti-inflammatory effect was investigated by Carrageenan-induced paw edema method. Results: The extract significantly inhibited both the first and second phases of formalin-induced nociception in mice at the dose of 100 mg/kg compared to the control group. In acetic acid-induced writhing test and hot plate method, the extract significantly reduced pain behavior in all doses (25, 50 and 100 mg/kg). The antinociceptive activity of the extract was significa...

Journal of Medicinal Plants, 2021

Background: Proper wound management which improves the quality of life and reduces patient costs ... more Background: Proper wound management which improves the quality of life and reduces patient costs is required. Lamium album L. has been used in traditional medicine to heal skin wounds and recent studies show anti-inflammatory, haemostatic and antimicrobial properties of this plant. Objective: The effect of the ointment containing methanol extract of L. album aerial parts was evaluated on the full-thickness wound healing in rat model. Methods: Thirty-two Wistar rats (250-300 g) were used to be created a square full-thickness wound on the dorsal cervical area and randomly divided into four groups: I; control, II; ointment base, III; 1 % phenytoin cream and IV; 5 % L. album ointment. Wound size for determination of the percent of wound healing was measured on days 3, 5, 7 and 12 of the experiment. The excisional biopsies were evaluated histopathologically on the 12 th day of treatment according to the Abramov score method. Results: The herbal ointment significantly increased fibroblast maturation, collagen deposition and neovascularization compared to the control group. The wound healing rate was significantly increased in the group treated with L. album ointment, same as phenytoin group, on days 3, 7 and 12. Based on the spectrophotometric analysis, the extract contains phenol and flavonoid compounds. Conclusion: It seems that L. album could be considered as a new candidate for further studies in the field of wound healing.

Advanced Biomedical Research, 2020

Background: Toxoplasma gondii (T. gondii) is a protozoan parasite that infects a wide range of wa... more Background: Toxoplasma gondii (T. gondii) is a protozoan parasite that infects a wide range of warm-blooded animals and humans. The conventional anti-Toxoplasma treatments cause significant toxicity. Brassicaceae family contains several medicinal plants with anti-inflammatory, chemopreventive, insecticide, antibacterial, antiviral, and antiparasitic effects. In this study, the hydroalcoholic extract of some Brassicaceae species was investigated against T. gondii in vitro. Materials and Methods: Seeds of Alyssum homolocarpum, Lepidium perfoliatum, Lepidium sativum, and aerial parts of Nasturtium officinale and Capsella bursa-pastoris were extracted by maceration method using 80% ethanol. Vero cells were treated with different concentrations (5–600 μg/mL) of the extracts and pyrimethamine (as positive control), and the cellular viability was verified. Next, Vero cells were infected by T. gondii tachyzoites (RH strain), and the viability of the infected cells was measured by a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: The 50% inhibitory concentration values were 5.1, 14.67, 32.49, 37.31, 71.35, and 2.63 μg/mL, and the selectivity indices were 8.06, 2.59, 0.74, 0.78, 0.65 (P < 0.05 compared with positive control), and 3.03 for L. sativum, L. perfoliatum, N. officinale, A. homolocarpum, C. bursa-pastoris, and pyrimethamine, respectively. Conclusion: The results of this study demonstrated that the hydroalcoholic extracts of L. sativum and L. perfoliatum have the promising anti-Toxoplasma activity by growth inhibition of T. gondii tachyzoites in infected cells.

Journal of Traditional and Complementary Medicine, 2017

Gentiana, a cosmopolitan and important genus of the Gentianaceae family, comprises 400 species di... more Gentiana, a cosmopolitan and important genus of the Gentianaceae family, comprises 400 species distributed among the world. Based on the studies of Iranian traditional medicine texts, there are some promising bioactivities for this genus that is unknown in modern medicine and some of them are still the basis of new remedies. In traditional medicine texts, Gentiana's different exclusive forms of preparations are effective for treatment of some disorders such as menstrual over-bleeding, conjunctivitis, vitiligo, animals venom poisoning, injuries, infected wounds, pain and swelling of liver, spleen, stomach and sprains of muscles. There are some activities that are the same in traditional and modern medicine such as anti-inflammatory, hepatoprotective and diuretic effects. Phytochemical investigations on the title genus have led to characterization many secondary metabolites. Secoiridoidal and iridoid glycosides such as gentiopicroside, xanthones, monoterpene alkaloid; polyphenol and flavones are the constituents that have been shown the pharmacological activities in different gentian species. This article studies the Gentiana according to the Iranian traditional and modern medicine.

Pharmacognosy Journal, 2015

Seed oil chemical composition of Platychaete aucheri (Boiss.

Journal of Medicinal Plants, 2020

Background: The genus Colchicum (Colchicaceae) is a perennial and monocotyledon flowering plant t... more Background: The genus Colchicum (Colchicaceae) is a perennial and monocotyledon flowering plant that has more than 160 species in the world. In addition, this plant is an important medicinal plant in traditional and modern medicines. Objective: The aim of this study was to evaluate the phytochemical profiles and physicochemical properties, antioxidant and anti-inflammatory activities of Colchicum kurdicum (Bornm.) Stef. corm. Methods: Phytochemical profiles includedtotal tropolone alkaloid, total phenolic/total tannin and total flavonoid contents were determined by spectrophotometric method. Moreover, tropolone alkaloid profiles was analyzed by HPLC method. Physicochemical properties including macroscopic and organoleptic properties, solubility, foreign matter, ash values and heavy metals were evaluated based on pharmacopeial protocoles. In addition, in vitro anti-inflammatory and antioxidant activities of the plant were determined. Results: Total tropolone alkaloids, phenol, tannin and flavonoid contents of the corm were estimated to be 0.652, 0.426, 0.052 and 0.325 g/100 g corm. In HPLC analysis, N-deacetyl-N-formyl colchicine, colchifoline, colchicoside and cornigerine were calculated as highest bioactive tropolone alkaloids. Physicochemical properties were determined within an acceptable range for the pharmacopoeia. Moreover, the anti-inflammatory and antioxidant activities in 10 mg/ml of methanolic extract were estimated 84.7 % and 94.8 %, respectively. Conclusion: The corm of the Colchicum kurdicum had the main bioactive compounds. In addition, these compounds have the valuable biological activities as anti-inflammatory and antioxidant activities.

Food Science and Nutrition, Jul 4, 2023

PubMed, Feb 1, 2018

Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species ... more Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species distributed among the world. Based on the studies of Persian medicine texts, there are some promising bioactivities for this genus that is unknown in modern medicine and some of them are still the basis of new remedies. This article presents Arnebia according to the most important ancient information by the most famous Persian medicine books like Makhzan Al Advieh, Tohfat Al-Momenin, Al-Qanun, Al-Seidaneh and Ekhtiarate Badiei. A search of electronic databases including PubMed, Scopus, Science Direct, and Google Scholar was done to find articles published between 1991 and 2017 on pharmacology and phytochemistry of Arnebia spp. In Persian medicine texts, Arnebia's different exclusive forms of preparations are effective for treatment of some disorders such as diarrhoea, amenorrhea, gout, kidney stone, jaundice, chronic fever and burn wounds. There are some activities that are the same in Persian and modern medicine research such as burn wound healing and fever amelioration. Phytochemical investigations on the title genus have led to characterization of many secondary metabolites. Naphthoquinones such as alkannins, shikonins, and their derivatives are the major constituents that have shown pharmacological activities in different Arnebia species. Among the major properties of Arnebia, only two of them (burn healing and fever amelioration) were investigated in modern medicine. The major aforementioned properties discussed in details in ancient sources might be a novel research sources leading to important discoveries in clinical usages of Arnebia.

South African Journal of Botany, May 1, 2021

Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with rema... more Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with remarkable protective activities against various chemical carcinogenesis and toxicities. Protective role of the hydroalcoholic extract and desulfo glucosinolate fraction from seeds of Lepidium perfoliatum were evaluated against CCl4-induced hepatotoxicity in BALB/c mice by determination of oxidative stress biomarkers and liver histopathological feature. The oral administration of different doses of the extract and desulfo glucosinolate fraction exhibited significant decrease in ROS and MDA contents of mitochondria in a dose-dependent manner, especially the doses of 200 mg/kg of the extract and 10 mg/kg of the fraction. GSH content was significantly increased (p

Turkish journal of pharmaceutical sciences, Feb 1, 2023

Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of disea... more Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of diseases in humans. Cytotoxicity and emergence of new strains resistance to the current anti-leishmanial drugs have encouraged researchers to focus on new resources. Glucosinolates (GSL) are found mainly in the Brassicaceae family with potential cytotoxic and anti-parasitic properties. The present study reports in vitro antileishmanial activity of the GSL fraction from Alyssum linifolium seeds against Leishmania major. Materials and Methods: The GSL fraction was prepared by ion-exchange and reversed-phase chromatography. For the assessment of antileishmanial activity, the promastigotes and amastigotes of L. major were treated with different concentrations of the fraction (75-625 μg/mL). Results: The IC 50 was 245 μg/mL for anti-promastigote effect of the GSL fraction and 250 μg/mL for its anti-amastigote effect that had a significant difference (p<0.05) with both glucantime and amphotericin B. The selectivity index of the GSL fraction (15.8), to glucantime and amphotericin B, was greater than 10, indicating the selective effect of this fraction against L. major amastigotes. Glucoiberverin was the major constituent of the GSL fraction characterized using nuclear magnetic resonance and electron ionization-mass spectrometry spectra. Based on gas chromatographymass spectrometry data, iberverin and iberverin nitrile, the hydrolysis constituents from glucoiberverin, included 76.91% of the total seed volatiles. Conclusion: The results suggest that GSLs like glucoiberverin could be considered a new promising candidate for further studies on antileishmanial activity.

DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2019

Background and objectives: Traditionally, Geum species from Rosaceae family have been used for tr... more Background and objectives: Traditionally, Geum species from Rosaceae family have been used for treating inflammatory disorders. Geum iranicum Khatamsaz is endemic to Iran. The aim of this study was to investigate the antinociceptive and anti-inflammatory activities of Geum iranicum methanol extract G. iranicum methanol extract using classical models. Methods: The methanol extract of G. iranicum roots was evaluated for antinociceptive activity by acetic acid-induced writhing, formalin and hot-plate tests in male Swiss albino mice. The anti-inflammatory effect was investigated by Carrageenan-induced paw edema method. Results: The extract significantly inhibited both the first and second phases of formalin-induced nociception in mice at the dose of 100 mg/kg compared to the control group. In acetic acid-induced writhing test and hot plate method, the extract significantly reduced pain behavior in all doses (25, 50 and 100 mg/kg). The antinociceptive activity of the extract was significantly reduced by naloxone (4 mg/kg). The anti-inflammatory activity of the extract was found to be dose dependent. The extract at the dose of 100 mg/kg exhibited significant reduction of paw edema in all surveyed times. Conclusion: The results showed that the methanol extract of G. iranicum roots possessed central analgesic activity via modulation of opioid receptors as well as antiinflammatory activity. The observed effects could be attributed to the presence of constituents like triterpenoids, eugenol, sucrose and tannins in the extract.

Food Science & Nutrition

South African Journal of Botany

Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with rema... more Abstract Glucosinolates are the main bioactive compounds present in Brassicaceae family with remarkable protective activities against various chemical carcinogenesis and toxicities. Protective role of the hydroalcoholic extract and desulfo glucosinolate fraction from seeds of Lepidium perfoliatum were evaluated against CCl4-induced hepatotoxicity in BALB/c mice by determination of oxidative stress biomarkers and liver histopathological feature. The oral administration of different doses of the extract and desulfo glucosinolate fraction exhibited significant decrease in ROS and MDA contents of mitochondria in a dose-dependent manner, especially the doses of 200 mg/kg of the extract and 10 mg/kg of the fraction. GSH content was significantly increased (p

Turkish Journal of Pharmaceutical Sciences

Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of disea... more Objectives: The intracellular parasitic protozoan, Leishmania spp., causes several forms of diseases in humans. Cytotoxicity and emergence of new strains resistance to the current anti-leishmanial drugs have encouraged researchers to focus on new resources. Glucosinolates (GSL) are found mainly in the Brassicaceae family with potential cytotoxic and anti-parasitic properties. The present study reports in vitro antileishmanial activity of the GSL fraction from Alyssum linifolium seeds against Leishmania major. Materials and Methods: The GSL fraction was prepared by ion-exchange and reversed-phase chromatography. For the assessment of antileishmanial activity, the promastigotes and amastigotes of L. major were treated with different concentrations of the fraction (75-625 μg/mL). Results: The IC 50 was 245 μg/mL for anti-promastigote effect of the GSL fraction and 250 μg/mL for its anti-amastigote effect that had a significant difference (p<0.05) with both glucantime and amphotericin B. The selectivity index of the GSL fraction (15.8), to glucantime and amphotericin B, was greater than 10, indicating the selective effect of this fraction against L. major amastigotes. Glucoiberverin was the major constituent of the GSL fraction characterized using nuclear magnetic resonance and electron ionization-mass spectrometry spectra. Based on gas chromatographymass spectrometry data, iberverin and iberverin nitrile, the hydrolysis constituents from glucoiberverin, included 76.91% of the total seed volatiles. Conclusion: The results suggest that GSLs like glucoiberverin could be considered a new promising candidate for further studies on antileishmanial activity.

Research Journal of Pharmacognosy, 2018

Jaundice is recognized by increased bilirubin level of blood and yellow appearance in skin, scler... more Jaundice is recognized by increased bilirubin level of blood and yellow appearance in skin, sclera and mucous membrane. Natural products have been used for treatment of jaundice for a long time and Persian Medicine can be a good source of natural drugs for this purpose. This study was done to find the materia medica used for jaundice based on Persian medicine (PM) texts including The Canon of Medicine (al-qanun), Al Hawi Fi Al Tibb, Tuhfat al-Mu’minin, Hedayat al-Motealemin fi Tibb, Zakhireye Khwarazmshahi, Al-Abniah 'an Haqaeq al-Adwia, Makhzan ul-Adwia and Ikhtiyarat Badie. One hundred eleven materia medica belonging to 51 families were identified as herbal remedies for treating jaundice. Apiaceae, Asteraceae, Cucurbitaceae, Fabaceae, Lamiaceae, Rosaceae and Zingiberaceae were the most dominant families. The results of this study may be present sources for pharmacological studies and development of new herbal medicines for jaundice. Asparagus, echium, arnebia, chicory, citron ...

Annals of parasitology, 2020

Leishmaniosis is one of the most serious public health concern with a worldwide distribution. Sin... more Leishmaniosis is one of the most serious public health concern with a worldwide distribution. Since the current treatments of leishmaniosis are toxic and expensive, frequent studies have been conducted to investigate the benefits of new resources such as medicinal plants for treatment of this infectious disease. Recent studies revealed the antiparasitic potential of Rhus coriaria. Here we investigated the potential antileishmanial and antibacterial activities of the hydroalcoholic extract of R. coriaria fruits. The fruits were extracted using 80% methanol by maceration method. The concentrations of 312, 156, 78, and 37 μg/ml of the extract were added separately to the wells containing Leishmania major (L. major) promastigotes and amastigotes. Amphotericin B was considered as positive control. Finally, the death rate was determined for the extract-treated parasites as compared to the non-treated parasite. The antibacterial activity was evaluated by measurement of the minimum inhibito...

Medicinski glasnik : official publication of the Medical Association of Zenica-Doboj Canton, Bosnia and Herzegovina, 2018

Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species ... more Arnebia, a sub cosmopolitan and important genus of the Boraginaceae family, comprises 25 species distributed among the world. Based on the studies of Persian medicine texts, there are some promising bioactivities for this genus that is unknown in modern medicine and some of them are still the basis of new remedies. This article presents Arnebia according to the most important ancient information by the most famous Persian medicine books like Makhzan Al Advieh, Tohfat Al-Momenin, Al-Qanun, Al-Seidaneh and Ekhtiarate Badiei. A search of electronic databases including PubMed, Scopus, Science Direct, and Google Scholar was done to find articles published between 1991 and 2017 on pharmacology and phytochemistry of Arnebia spp. In Persian medicine texts, Arnebia's different exclusive forms of preparations are effective for treatment of some disorders such as diarrhoea, amenorrhea, gout, kidney stone, jaundice, chronic fever and burn wounds. There are some activities that are the same ...

Journal of Mazandaran University of Medical Sciences, 2021

Background and purpose: There are few treatment options available for treatment of toxoplasmosis ... more Background and purpose: There are few treatment options available for treatment of toxoplasmosis and effective drugs have serious toxic effects. In this study, the in vivo and in vitro anti-toxoplasma activities of Heracleum persicum and Foeniculum vulgare fruits essential oils were investigated. Materials and methods: In vitro, Vero cells were incubated with different concentrations of essential oils or pyrimethamine (positive control) and the cellular viability was determined. Next, Vero cells were infected with T. gondii (RH strain) and treated with agents. Then, the CC50, IC50, and selectivity index (SI) were calculated. Moreover, in vivo, the effect of oils on survival times of Balb/c mice infected with T. gondii were determined. Results: In vitro results showed that the oils exhibited less cell toxicity than pyrimethamine. The selective index was 2.94, 6.96, and 3.06 for Heracleum persicum, Foeniculum vulgare, and pyrimethamine, respectively. Also, the infected mice treated wi...

Research Journal of Pharmacognosy, 2020

Background and objectives: Colchicum kurdicum (Bornm.) Stef. is a flowering perennial monocotyled... more Background and objectives: Colchicum kurdicum (Bornm.) Stef. is a flowering perennial monocotyledon plant that has many important bioactive compounds especially colchicine and colchicine derivatives. In this study, the ultrasound-assisted acidic-solvent extraction method coupled with response-surface method was presented as the successful method for large scale extraction of colchicine as an alkaloid compound from Colchicum kurdicum. Methods: According to the literatures, methanol/deionized water (70:30) solvent system was selected for the extraction. In addition, the response-surface method was used for analysis and optimization of colchicine extraction by ultrasonic-assisted acidic-solvent extraction method. Subsequently, colchicine was extracted using this method and the effects of solvent pH, extraction time, solvent/plant ratio, power, and temperature were evaluated. Results: After all analysis procedures, 0.99 mg colchicine/g dried corms was achieved with the following conditi...

Research Journal of Pharmacognosy, 2019

Physalis, the fifth largest genus of the Solanaceae family, consists many species which are used ... more Physalis, the fifth largest genus of the Solanaceae family, consists many species which are used as food and natural remedies. Phytochemical investigations on Physalis species have led to characterization of important secondary metabolites such as withaphysalins, physalins and phytosterols which are responsible for wide range of pharmacological effects such as potent cytotoxic activity against various type of human cancer cell lines, immunomodulatory and cancer chemopreventive effects. Theyare widely used in the indigenous system of medicine for treatment of kidney and urinary disorders, leishmaniasis, inflammations, skin infections and etc. Based on Iranian traditional medicine texts, Physalis is effective for treatment of several disorders such as asthma, liver, kidney and bladder dysfunctions, wounds, hemorrhoids, and helminthic diseases. Based on recent surveys, many of the above properties have not been investigated so far except the for the healing effects on liver dysfunction...

Research Journal of Pharmacognosy, 2019

Background and objectives:Traditionally, Geum species from Rosaceae family have been used for tre... more Background and objectives:Traditionally, Geum species from Rosaceae family have been used for treating inflammatory disorders. Geum iranicum Khatamsaz is endemic to Iran. The aim of this study was to investigate the antinociceptive and anti-inflammatory activities of Geum iranicum methanol extract G. iranicum methanol extract using classical models. Methods: The methanol extract of G. iranicum roots was evaluated for antinociceptive activity by acetic acid-induced writhing, formalin and hot-plate tests in male Swiss albino mice. The anti-inflammatory effect was investigated by Carrageenan-induced paw edema method. Results: The extract significantly inhibited both the first and second phases of formalin-induced nociception in mice at the dose of 100 mg/kg compared to the control group. In acetic acid-induced writhing test and hot plate method, the extract significantly reduced pain behavior in all doses (25, 50 and 100 mg/kg). The antinociceptive activity of the extract was significa...

Journal of Medicinal Plants, 2021

Background: Proper wound management which improves the quality of life and reduces patient costs ... more Background: Proper wound management which improves the quality of life and reduces patient costs is required. Lamium album L. has been used in traditional medicine to heal skin wounds and recent studies show anti-inflammatory, haemostatic and antimicrobial properties of this plant. Objective: The effect of the ointment containing methanol extract of L. album aerial parts was evaluated on the full-thickness wound healing in rat model. Methods: Thirty-two Wistar rats (250-300 g) were used to be created a square full-thickness wound on the dorsal cervical area and randomly divided into four groups: I; control, II; ointment base, III; 1 % phenytoin cream and IV; 5 % L. album ointment. Wound size for determination of the percent of wound healing was measured on days 3, 5, 7 and 12 of the experiment. The excisional biopsies were evaluated histopathologically on the 12 th day of treatment according to the Abramov score method. Results: The herbal ointment significantly increased fibroblast maturation, collagen deposition and neovascularization compared to the control group. The wound healing rate was significantly increased in the group treated with L. album ointment, same as phenytoin group, on days 3, 7 and 12. Based on the spectrophotometric analysis, the extract contains phenol and flavonoid compounds. Conclusion: It seems that L. album could be considered as a new candidate for further studies in the field of wound healing.

Advanced Biomedical Research, 2020

Background: Toxoplasma gondii (T. gondii) is a protozoan parasite that infects a wide range of wa... more Background: Toxoplasma gondii (T. gondii) is a protozoan parasite that infects a wide range of warm-blooded animals and humans. The conventional anti-Toxoplasma treatments cause significant toxicity. Brassicaceae family contains several medicinal plants with anti-inflammatory, chemopreventive, insecticide, antibacterial, antiviral, and antiparasitic effects. In this study, the hydroalcoholic extract of some Brassicaceae species was investigated against T. gondii in vitro. Materials and Methods: Seeds of Alyssum homolocarpum, Lepidium perfoliatum, Lepidium sativum, and aerial parts of Nasturtium officinale and Capsella bursa-pastoris were extracted by maceration method using 80% ethanol. Vero cells were treated with different concentrations (5–600 μg/mL) of the extracts and pyrimethamine (as positive control), and the cellular viability was verified. Next, Vero cells were infected by T. gondii tachyzoites (RH strain), and the viability of the infected cells was measured by a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: The 50% inhibitory concentration values were 5.1, 14.67, 32.49, 37.31, 71.35, and 2.63 μg/mL, and the selectivity indices were 8.06, 2.59, 0.74, 0.78, 0.65 (P < 0.05 compared with positive control), and 3.03 for L. sativum, L. perfoliatum, N. officinale, A. homolocarpum, C. bursa-pastoris, and pyrimethamine, respectively. Conclusion: The results of this study demonstrated that the hydroalcoholic extracts of L. sativum and L. perfoliatum have the promising anti-Toxoplasma activity by growth inhibition of T. gondii tachyzoites in infected cells.

Journal of Traditional and Complementary Medicine, 2017

Gentiana, a cosmopolitan and important genus of the Gentianaceae family, comprises 400 species di... more Gentiana, a cosmopolitan and important genus of the Gentianaceae family, comprises 400 species distributed among the world. Based on the studies of Iranian traditional medicine texts, there are some promising bioactivities for this genus that is unknown in modern medicine and some of them are still the basis of new remedies. In traditional medicine texts, Gentiana's different exclusive forms of preparations are effective for treatment of some disorders such as menstrual over-bleeding, conjunctivitis, vitiligo, animals venom poisoning, injuries, infected wounds, pain and swelling of liver, spleen, stomach and sprains of muscles. There are some activities that are the same in traditional and modern medicine such as anti-inflammatory, hepatoprotective and diuretic effects. Phytochemical investigations on the title genus have led to characterization many secondary metabolites. Secoiridoidal and iridoid glycosides such as gentiopicroside, xanthones, monoterpene alkaloid; polyphenol and flavones are the constituents that have been shown the pharmacological activities in different gentian species. This article studies the Gentiana according to the Iranian traditional and modern medicine.

Pharmacognosy Journal, 2015

Seed oil chemical composition of Platychaete aucheri (Boiss.

Journal of Medicinal Plants, 2020

Background: The genus Colchicum (Colchicaceae) is a perennial and monocotyledon flowering plant t... more Background: The genus Colchicum (Colchicaceae) is a perennial and monocotyledon flowering plant that has more than 160 species in the world. In addition, this plant is an important medicinal plant in traditional and modern medicines. Objective: The aim of this study was to evaluate the phytochemical profiles and physicochemical properties, antioxidant and anti-inflammatory activities of Colchicum kurdicum (Bornm.) Stef. corm. Methods: Phytochemical profiles includedtotal tropolone alkaloid, total phenolic/total tannin and total flavonoid contents were determined by spectrophotometric method. Moreover, tropolone alkaloid profiles was analyzed by HPLC method. Physicochemical properties including macroscopic and organoleptic properties, solubility, foreign matter, ash values and heavy metals were evaluated based on pharmacopeial protocoles. In addition, in vitro anti-inflammatory and antioxidant activities of the plant were determined. Results: Total tropolone alkaloids, phenol, tannin and flavonoid contents of the corm were estimated to be 0.652, 0.426, 0.052 and 0.325 g/100 g corm. In HPLC analysis, N-deacetyl-N-formyl colchicine, colchifoline, colchicoside and cornigerine were calculated as highest bioactive tropolone alkaloids. Physicochemical properties were determined within an acceptable range for the pharmacopoeia. Moreover, the anti-inflammatory and antioxidant activities in 10 mg/ml of methanolic extract were estimated 84.7 % and 94.8 %, respectively. Conclusion: The corm of the Colchicum kurdicum had the main bioactive compounds. In addition, these compounds have the valuable biological activities as anti-inflammatory and antioxidant activities.