Laila Fayyaz - Academia.edu (original) (raw)

Papers by Laila Fayyaz

Sarhad Journal of Agriculture, 2022

P ulses are considered as the second most important crop after cereals fulfilling dietary require... more P ulses are considered as the second most important crop after cereals fulfilling dietary requirements for humans in many parts of the world (Maphosa and Jideani, 2017). Chickpea (Cicer arietinum L.) is valuable for its cheapest source of protein and an inexpensive meat alternative. Various types of chickpeas are commonly known as gram, garbanzo or garbanzo bean. Chickpea has a great nutritional value. Their seeds provide proteins and carbohydrates, together constituting ~ 80% of the total dry seed mass. Comparative to common beans (Phaseolus vulgaris L.) and field peas (Pisum sativum L.) chickpea has higher Abstract | Phosphorus (P) is one of the essential nutrients needed for plant growth and development. The P has a significant ecological and economic importance; therefore, its application is considered to maximize the yield of various crops, including chickpea. The current experiment was performed to assess the impact of phosphorus application on yield of 15 chickpea genotypes during the growing season of 2017-18 at the University of Agriculture, Peshawar. A randomized complete block design was used with three replications under two treatments. One treatment was a control without phosphorous application, while the second treatment was application of phosphorous at a rate of 35.3 kg (P 2 O 5) ha-1. Data were documented on plant height, pod number plant-1 , seed number pod-1 , seed number plant-1 , 100-seed weight, seed yield and harvest index. Amongst genotypes, highly significant variation was observed for the studied characters. Similarly, significant results were obtained for genotype by environment (GE) interaction for all traits except plant height. Application of phosphorus enormously improved seed yield in all chickpea genotypes. Based on mean data, chickpea plants with P application produced more seed number plant-1 (60.9), pods plant-1 (57.3) and seed yield (557.2 kg ha-1). Higher seed yields were produced by genotypes NDC-4-20-1 (1004 kg ha-1) and NKC-5-S-15 (851 kg ha-1). Seed yield had significantly negative phenotypic correlation with plant height. Similarly at genotypic level, it was significantly correlated with seed number pod-1 while negatively with 100-seed weight. Among the tested genotypes, NDC-4-20-1 and NKC-5-S-15 were identified as better performing with phosphorus application while genotype NKC-5-S-12 followed by NDC-4-20-1 performed well in both environments. Therefore, the screened lines could be used in various chickpea breeding programs for higher seed yield. Considering current findings, this study suggests that application of phosphorus to chickpea fields is recommendable for higher seed yield and hence is justified from economic perspective.

Microsatellites markers were tested for their ability to distinguish genomic distribution of the ... more Microsatellites markers were tested for their ability to distinguish genomic distribution of the Brassica species of the U Triangle and E. sativa. The objectives of the present study were to investigate the genetic diversity of six Brassica species from U-Triangle (representing three genomes, A, B, C) and one from genus Eruca and to identify promising sources of genetic variation for breeding purposes. A total of 54 SSR markers were analyzed in order to detect variation between and within the selected genomes. Three primer pairs depicted the greatest genetic diversity showing 97% polymorphism between Brassica and Eruca genomes (2.55 alleles per locus). Polymorphic Information Content (PIC) values ranged from 0.40 (SSR primer Na14-DO7) to 0.79 (NA10-G09). For comparison within Brassica genomes and Eruca, all the genomes were grouped in three modules i.e., ABE, ACE and BCE (Fig. 1). The tetraploid originating from their parental diploids alongwith Eruca was considered in the same module. For the estimation of relatedness within and among genomes, dice coefficients were computed as a measure of genetic similarity matrix. On the basis of genetic distances, dendrogram was constructed through cluster analysis. Two major clusters at coefficient of similarity level (0.47) were observed. One cluster comprised of all Brassica genomes and their accessions, while another consisting of all accessions of Eruca genome. The cluster containing Brassica genomes was further subdivided into four subgroups that contained diploid and tetraploid species in a way that tetraploid species were grouped in between their diploid parental species with varying genetic distances. Present findings confirmed the validity of SSR markers in genomic studies. Fig. 1. Diagrammatic representation of modules with their respective genomes and species.

Genetika, 2014

Brassica species are vulnerable to narrow genetic base due to the ignorance of their wild relativ... more Brassica species are vulnerable to narrow genetic base due to the ignorance of their wild relatives which possess many superior characters. This study was aimed to explore the genetic diversity in five Brassica species from U triangle as well as in their wild relative Eruca sativa. For the complete insight of genetic diversity, four accessions, each from five species of genus Brassica along with one species of Eruca collected from different geographical locations (exotic and indigenous) were selected. Six yield associated parameters viz., primary branches plant-1, plant height, main raceme length, silique length, silique width and silique main raceme-1 were studied. Highly significant variations among all species were observed. Mean performance showed that wild relative E. sativa was superior for primary branches plant -1 and plant height, which are the main yield associated traits. In case of Brassica species, B. campestris gave the lengthiest main racemes, B. nigra produced more s...

Legume Genomics and Genetics, 2013

The present studies were carried out to assess the genetic variability, heritability and genetic ... more The present studies were carried out to assess the genetic variability, heritability and genetic advance for different characters in 22 diverse genotypes of bush cowpea. The experiment was conducted in randomized complete block design with three replications during the period 2011-2012 at the Department of Olericulture, College of Agriculture, Vellayani, Kerala, India. The analysis of variance indicated the prevalence of sufficient genetic variation among the genotypes from all the characters studied. Among the genotypes VU 6 (310.41 g) was the highest yielder. Pods per plant were highest in VU 8 (70.30) and pod weight in VU 20 (12.44 g). The high phenotypic coefficient of variation and genotypic coefficient of variation were observed for pod weight, plant height, and pod length. High heritability coupled with high genetic advance were observed for all characters studied, except days to first flowering and days to first harvest indicating these characters are governed by additive gene action. Hence, direct selection may be followed for the improvement of vegetable cowpea for these characters.

and M. Afzal (2014): Genetic diversity in Brassica species and Eruca sativa for yield associated ... more and M. Afzal (2014): Genetic diversity in Brassica species and Eruca sativa for yield associated parameters- Genetika, vol., No.46, No.2, 537-543. Brassica species are vulnerable to narrow genetic base due to the ignorance of their wild relatives which possess many superior characters. This study was aimed to explore the genetic diversity in five Brassica species from U triangle as well as in their wild relative Eruca sativa. For the complete insight of genetic diversity, four accessions, each from five species of genus Brassica along with one species of Eruca collected from different geographical locations (exotic and indigenous) were selected. Six yield associated parameters viz., primary branches plant-1, plant height, main raceme length, silique length, silique width and silique main raceme-1 were studied. Highly significant variations among all species were observed. Mean performance showed that wild relative E. sativa was superior for primary branches plant-1 and plant height,...

Assessment of variability and the heritable proportion of this variability are crucial to estimat... more Assessment of variability and the heritable proportion of this variability are crucial to estimate the genetic advance in oilseed Brassica improvement, as in all crops. These may be variable in different segregating populations, including the F2 populations of different crosses and should be studied to select the appropriate segregating population for further improvement. We; therefore, report on the estimation of variability, heritability and genetic advance for ten parental lines and the four intraspecific and four interspecific F2 populations of brassica at New Developmental Farm, of the University of Agriculture, Peshawar for biochemical parameters. The experimental material studied was grown in the 1st week of October, 2010 in a randomized complete block design with three replications. In all genotypes highly significant (p≤0.01) differences were recorded for protein, glucosinolates, oleic acid, oil, erucic acid and linolenic acid content. Parental genotypes N-507, N-542 and N-...

Estimation of genetic parameters in the context of trait characterization is an essential compone... more Estimation of genetic parameters in the context of trait characterization is an essential component of future targeted crop improvement programs. Collection of knowledge about genetic behavior such as genetic variability and heritability etc., of the germplasm is the basic step for initiation of any breeding program. Genetic variability and Broad sense heritability for various seed quality traits in 10 brassica genotypes and their 12 F2 progenies comprising of introgressed hybrids were studied. The genotypes had highly significant variation for oil content, protein, glucosinolates contents, oleic, linolenic and erucic acid contents. Glucosinolates content and erucic acid showed high heritability in all F2 populations, while rest of the traits showed variable trends. The cross combination 547 x 118 (B. napus x B. campestris) proved to be a good interspecific hybrid that had high proportion of introgression and has high heritability for beneficial traits. The individual plants having ...

Genetic variability for morphological and biochemical traits among six advanced lines (F10:11) of... more Genetic variability for morphological and biochemical traits among six advanced lines (F10:11) of brassica was studied at The University of Agriculture Peshawar during crop season of 2012-13. These lines were developed through interspecific hybridization. Significant differences at (p≤0.01) for plant height, main, pods main raceme -1 , pod length, seed yield plant -1 and protein content at (p≤0.05) for 100-seed weight, oil content were recorded. The advanced line, AUP-05 produced the maximum seed yield plant -1 (19.73 g), protein content (24.56%), 100-seed weight (0.64 g). Advanced line AUP-04 had the highest erucic acid (50.31%), linolenic acid (10.60%) and was late maturing (179.33). Advanced line AUP-06 produced the high oil content (48.82%). Advanced line AUP-03 produced comparatively longer main raceme (69.32 cm). Environmental variance was smaller than genotypic variance for majority of the traits. Genotypic and phenotypic coefficients of variation ranged from 2.45 to 25.67% a...

Sarhad Journal of Agriculture

Pakistan Journal of Botany

Genetic diversity and relationship of F 2 segregating progenies of interspecific crosses between ... more Genetic diversity and relationship of F 2 segregating progenies of interspecific crosses between B. napus N-501/B. campestris C-118 were studied. A set of 90 genotypes (2 parental lines and their 88 F 2 progenies) was characterized separately using 24 microsatellite or SSR markers to cover the diversity as broadly as possibly present in them. In initial screening only 12 out of 24 SSR primers combination amplified DNA fragments, while the remaining 12 SSR primers did not amplify DNA fragment therefore those 12 SSR molecular markers were not used for further analysis. The 12 SSR primer combinations generated a total of 33 alleles, of that 32 were polymorphic loci, whereas only one was monomorphic locus. Primers BRMS-19 and BRMS-40 were highly polymorphic producing 4 bands each. Primer Ra2-D04 was less polymorphic and it produced only one band. The proportion of polymorphic loci was 95.83% which indicates high genetic diversity among the progenies. The average number of polymorphic al...

Plant Disease

The European grapevine (Vitis vinifera L.) has been cultivated in North America for about 500 yea... more The European grapevine (Vitis vinifera L.) has been cultivated in North America for about 500 years. One of the major limitations to its culture is the powdery mildew (PM) fungus, Erysiphe necator Schw. This study reports on the most extensive screening of Vitis species from the southwestern United States and northern Mexico for resistance to PM, testing 147 accessions of 13 Vitis species. In addition, Vitis vinifera cv. Carignane, a highly susceptible wine grape cultivar, was used as a reference to evaluate the effect of the inoculum 14 days postinoculation. Inoculation was done with a vacuum-operated settling tower using a broadly virulent isolate of E. necator, the C-strain. Resistant accessions (nine), moderately susceptible accessions (39), and highly susceptible accessions (99) were detected. The resistant accessions were then inoculated with an additional fungal isolate, e1-101, and they retained their resistance. Vitis species susceptibility was not associated with a North-S...

Estimation of genetic parameters in the context of trait characterization is an essential compone... more Estimation of genetic parameters in the context of trait characterization is an essential component of future targeted crop improvement programs. Collection of knowledge about genetic behavior such as genetic variability and heritability etc., of the germplasm is the basic step for initiation of any breeding program. Genetic variability and Broad sense heritability for various seed quality traits in 10 brassica genotypes and their 12 F 2 progenies comprising of introgressed hybrids were studied. The genotypes had highly significant variation for oil content, protein, glucosinolates contents, oleic, linolenic and erucic acid contents. Glucosinolates content and erucic acid showed high heritability in all F 2 populations, while rest of the traits showed variable trends. The cross combination 547 x 118 (B. napus x B. campestris) proved to be a good interspecific hybrid that had high proportion of introgression and has high heritability for beneficial traits. The individual plants having combination of desirable traits were also identified from the F 2 populations.

Sarhad Journal of Agriculture, 2022

P ulses are considered as the second most important crop after cereals fulfilling dietary require... more P ulses are considered as the second most important crop after cereals fulfilling dietary requirements for humans in many parts of the world (Maphosa and Jideani, 2017). Chickpea (Cicer arietinum L.) is valuable for its cheapest source of protein and an inexpensive meat alternative. Various types of chickpeas are commonly known as gram, garbanzo or garbanzo bean. Chickpea has a great nutritional value. Their seeds provide proteins and carbohydrates, together constituting ~ 80% of the total dry seed mass. Comparative to common beans (Phaseolus vulgaris L.) and field peas (Pisum sativum L.) chickpea has higher Abstract | Phosphorus (P) is one of the essential nutrients needed for plant growth and development. The P has a significant ecological and economic importance; therefore, its application is considered to maximize the yield of various crops, including chickpea. The current experiment was performed to assess the impact of phosphorus application on yield of 15 chickpea genotypes during the growing season of 2017-18 at the University of Agriculture, Peshawar. A randomized complete block design was used with three replications under two treatments. One treatment was a control without phosphorous application, while the second treatment was application of phosphorous at a rate of 35.3 kg (P 2 O 5) ha-1. Data were documented on plant height, pod number plant-1 , seed number pod-1 , seed number plant-1 , 100-seed weight, seed yield and harvest index. Amongst genotypes, highly significant variation was observed for the studied characters. Similarly, significant results were obtained for genotype by environment (GE) interaction for all traits except plant height. Application of phosphorus enormously improved seed yield in all chickpea genotypes. Based on mean data, chickpea plants with P application produced more seed number plant-1 (60.9), pods plant-1 (57.3) and seed yield (557.2 kg ha-1). Higher seed yields were produced by genotypes NDC-4-20-1 (1004 kg ha-1) and NKC-5-S-15 (851 kg ha-1). Seed yield had significantly negative phenotypic correlation with plant height. Similarly at genotypic level, it was significantly correlated with seed number pod-1 while negatively with 100-seed weight. Among the tested genotypes, NDC-4-20-1 and NKC-5-S-15 were identified as better performing with phosphorus application while genotype NKC-5-S-12 followed by NDC-4-20-1 performed well in both environments. Therefore, the screened lines could be used in various chickpea breeding programs for higher seed yield. Considering current findings, this study suggests that application of phosphorus to chickpea fields is recommendable for higher seed yield and hence is justified from economic perspective.

Microsatellites markers were tested for their ability to distinguish genomic distribution of the ... more Microsatellites markers were tested for their ability to distinguish genomic distribution of the Brassica species of the U Triangle and E. sativa. The objectives of the present study were to investigate the genetic diversity of six Brassica species from U-Triangle (representing three genomes, A, B, C) and one from genus Eruca and to identify promising sources of genetic variation for breeding purposes. A total of 54 SSR markers were analyzed in order to detect variation between and within the selected genomes. Three primer pairs depicted the greatest genetic diversity showing 97% polymorphism between Brassica and Eruca genomes (2.55 alleles per locus). Polymorphic Information Content (PIC) values ranged from 0.40 (SSR primer Na14-DO7) to 0.79 (NA10-G09). For comparison within Brassica genomes and Eruca, all the genomes were grouped in three modules i.e., ABE, ACE and BCE (Fig. 1). The tetraploid originating from their parental diploids alongwith Eruca was considered in the same module. For the estimation of relatedness within and among genomes, dice coefficients were computed as a measure of genetic similarity matrix. On the basis of genetic distances, dendrogram was constructed through cluster analysis. Two major clusters at coefficient of similarity level (0.47) were observed. One cluster comprised of all Brassica genomes and their accessions, while another consisting of all accessions of Eruca genome. The cluster containing Brassica genomes was further subdivided into four subgroups that contained diploid and tetraploid species in a way that tetraploid species were grouped in between their diploid parental species with varying genetic distances. Present findings confirmed the validity of SSR markers in genomic studies. Fig. 1. Diagrammatic representation of modules with their respective genomes and species.

Genetika, 2014

Brassica species are vulnerable to narrow genetic base due to the ignorance of their wild relativ... more Brassica species are vulnerable to narrow genetic base due to the ignorance of their wild relatives which possess many superior characters. This study was aimed to explore the genetic diversity in five Brassica species from U triangle as well as in their wild relative Eruca sativa. For the complete insight of genetic diversity, four accessions, each from five species of genus Brassica along with one species of Eruca collected from different geographical locations (exotic and indigenous) were selected. Six yield associated parameters viz., primary branches plant-1, plant height, main raceme length, silique length, silique width and silique main raceme-1 were studied. Highly significant variations among all species were observed. Mean performance showed that wild relative E. sativa was superior for primary branches plant -1 and plant height, which are the main yield associated traits. In case of Brassica species, B. campestris gave the lengthiest main racemes, B. nigra produced more s...

Legume Genomics and Genetics, 2013

The present studies were carried out to assess the genetic variability, heritability and genetic ... more The present studies were carried out to assess the genetic variability, heritability and genetic advance for different characters in 22 diverse genotypes of bush cowpea. The experiment was conducted in randomized complete block design with three replications during the period 2011-2012 at the Department of Olericulture, College of Agriculture, Vellayani, Kerala, India. The analysis of variance indicated the prevalence of sufficient genetic variation among the genotypes from all the characters studied. Among the genotypes VU 6 (310.41 g) was the highest yielder. Pods per plant were highest in VU 8 (70.30) and pod weight in VU 20 (12.44 g). The high phenotypic coefficient of variation and genotypic coefficient of variation were observed for pod weight, plant height, and pod length. High heritability coupled with high genetic advance were observed for all characters studied, except days to first flowering and days to first harvest indicating these characters are governed by additive gene action. Hence, direct selection may be followed for the improvement of vegetable cowpea for these characters.

and M. Afzal (2014): Genetic diversity in Brassica species and Eruca sativa for yield associated ... more and M. Afzal (2014): Genetic diversity in Brassica species and Eruca sativa for yield associated parameters- Genetika, vol., No.46, No.2, 537-543. Brassica species are vulnerable to narrow genetic base due to the ignorance of their wild relatives which possess many superior characters. This study was aimed to explore the genetic diversity in five Brassica species from U triangle as well as in their wild relative Eruca sativa. For the complete insight of genetic diversity, four accessions, each from five species of genus Brassica along with one species of Eruca collected from different geographical locations (exotic and indigenous) were selected. Six yield associated parameters viz., primary branches plant-1, plant height, main raceme length, silique length, silique width and silique main raceme-1 were studied. Highly significant variations among all species were observed. Mean performance showed that wild relative E. sativa was superior for primary branches plant-1 and plant height,...

Assessment of variability and the heritable proportion of this variability are crucial to estimat... more Assessment of variability and the heritable proportion of this variability are crucial to estimate the genetic advance in oilseed Brassica improvement, as in all crops. These may be variable in different segregating populations, including the F2 populations of different crosses and should be studied to select the appropriate segregating population for further improvement. We; therefore, report on the estimation of variability, heritability and genetic advance for ten parental lines and the four intraspecific and four interspecific F2 populations of brassica at New Developmental Farm, of the University of Agriculture, Peshawar for biochemical parameters. The experimental material studied was grown in the 1st week of October, 2010 in a randomized complete block design with three replications. In all genotypes highly significant (p≤0.01) differences were recorded for protein, glucosinolates, oleic acid, oil, erucic acid and linolenic acid content. Parental genotypes N-507, N-542 and N-...

Estimation of genetic parameters in the context of trait characterization is an essential compone... more Estimation of genetic parameters in the context of trait characterization is an essential component of future targeted crop improvement programs. Collection of knowledge about genetic behavior such as genetic variability and heritability etc., of the germplasm is the basic step for initiation of any breeding program. Genetic variability and Broad sense heritability for various seed quality traits in 10 brassica genotypes and their 12 F2 progenies comprising of introgressed hybrids were studied. The genotypes had highly significant variation for oil content, protein, glucosinolates contents, oleic, linolenic and erucic acid contents. Glucosinolates content and erucic acid showed high heritability in all F2 populations, while rest of the traits showed variable trends. The cross combination 547 x 118 (B. napus x B. campestris) proved to be a good interspecific hybrid that had high proportion of introgression and has high heritability for beneficial traits. The individual plants having ...

Genetic variability for morphological and biochemical traits among six advanced lines (F10:11) of... more Genetic variability for morphological and biochemical traits among six advanced lines (F10:11) of brassica was studied at The University of Agriculture Peshawar during crop season of 2012-13. These lines were developed through interspecific hybridization. Significant differences at (p≤0.01) for plant height, main, pods main raceme -1 , pod length, seed yield plant -1 and protein content at (p≤0.05) for 100-seed weight, oil content were recorded. The advanced line, AUP-05 produced the maximum seed yield plant -1 (19.73 g), protein content (24.56%), 100-seed weight (0.64 g). Advanced line AUP-04 had the highest erucic acid (50.31%), linolenic acid (10.60%) and was late maturing (179.33). Advanced line AUP-06 produced the high oil content (48.82%). Advanced line AUP-03 produced comparatively longer main raceme (69.32 cm). Environmental variance was smaller than genotypic variance for majority of the traits. Genotypic and phenotypic coefficients of variation ranged from 2.45 to 25.67% a...

Sarhad Journal of Agriculture

Pakistan Journal of Botany

Genetic diversity and relationship of F 2 segregating progenies of interspecific crosses between ... more Genetic diversity and relationship of F 2 segregating progenies of interspecific crosses between B. napus N-501/B. campestris C-118 were studied. A set of 90 genotypes (2 parental lines and their 88 F 2 progenies) was characterized separately using 24 microsatellite or SSR markers to cover the diversity as broadly as possibly present in them. In initial screening only 12 out of 24 SSR primers combination amplified DNA fragments, while the remaining 12 SSR primers did not amplify DNA fragment therefore those 12 SSR molecular markers were not used for further analysis. The 12 SSR primer combinations generated a total of 33 alleles, of that 32 were polymorphic loci, whereas only one was monomorphic locus. Primers BRMS-19 and BRMS-40 were highly polymorphic producing 4 bands each. Primer Ra2-D04 was less polymorphic and it produced only one band. The proportion of polymorphic loci was 95.83% which indicates high genetic diversity among the progenies. The average number of polymorphic al...

Plant Disease

The European grapevine (Vitis vinifera L.) has been cultivated in North America for about 500 yea... more The European grapevine (Vitis vinifera L.) has been cultivated in North America for about 500 years. One of the major limitations to its culture is the powdery mildew (PM) fungus, Erysiphe necator Schw. This study reports on the most extensive screening of Vitis species from the southwestern United States and northern Mexico for resistance to PM, testing 147 accessions of 13 Vitis species. In addition, Vitis vinifera cv. Carignane, a highly susceptible wine grape cultivar, was used as a reference to evaluate the effect of the inoculum 14 days postinoculation. Inoculation was done with a vacuum-operated settling tower using a broadly virulent isolate of E. necator, the C-strain. Resistant accessions (nine), moderately susceptible accessions (39), and highly susceptible accessions (99) were detected. The resistant accessions were then inoculated with an additional fungal isolate, e1-101, and they retained their resistance. Vitis species susceptibility was not associated with a North-S...

Estimation of genetic parameters in the context of trait characterization is an essential compone... more Estimation of genetic parameters in the context of trait characterization is an essential component of future targeted crop improvement programs. Collection of knowledge about genetic behavior such as genetic variability and heritability etc., of the germplasm is the basic step for initiation of any breeding program. Genetic variability and Broad sense heritability for various seed quality traits in 10 brassica genotypes and their 12 F 2 progenies comprising of introgressed hybrids were studied. The genotypes had highly significant variation for oil content, protein, glucosinolates contents, oleic, linolenic and erucic acid contents. Glucosinolates content and erucic acid showed high heritability in all F 2 populations, while rest of the traits showed variable trends. The cross combination 547 x 118 (B. napus x B. campestris) proved to be a good interspecific hybrid that had high proportion of introgression and has high heritability for beneficial traits. The individual plants having combination of desirable traits were also identified from the F 2 populations.