Fazal Wadood - Academia.edu (original) (raw)

Papers by Fazal Wadood

Buffalo Bulletin, Sep 26, 2022

In this study, efforts were made to investigate fresh semen parameters and to select a suitable e... more In this study, efforts were made to investigate fresh semen parameters and to select a suitable extender for buffalo semen cryopreservation. Experiment I, fresh undiluted seminal parameters were determined while Experiment II, efficacy comparison of coconut water extender (CWE) with tris citric acid extender (TCAE) and skimmed milk extender (SME) was made. Four bulls single ejaculate was collected weekly for 5 and 10 weeks for Experiment I and II, respectively however osmotic pressure replicates were 16 and 6 for semen and seminal plasma, respectively. In Experiment I, each bull spermatozoa concentration, motility (%), semen volume and pooled semen percentage NAR, PMI, viability and MTT (3-(4, 5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction rate was checked. In Experiment II, pooled semen added to extenders and then equilibrated (4 o C) and filled to obtain 20×10 6 spermatozoa/0.5 ml straws before plunging in liquid nitrogen. Percentage thawed spermatozoa viability, normal acrosomal ridge (NAR), motility, DNA damage, plasma membrane integrity (PMI) and lipid peroxidation (nM) recorded. In Experiment I, seminal parameters as spermatozoa concentration (1226.43±71.48 million/mL), semen volume (2.84±0.14 mL), viability (90.05±0.71%), motility (77.13±0.71%), PMI (86.23±0.34%), NAR (94.67±0.30%) and MTT reduction rate (0.290±0.06) while osmotic pressure of seminal plasma (294.83±3.87 mOsm/ kg) and semen (290.87±2.58 mOsm/kg) was recorded. In Experiment II, TCAE higher (P<0.05) sperm motility noted compared to CWE and SME whereas percentage viability, NAR, PMI, DNA damage was non-significant. Lipid peroxidation compared to SME and TCAE was higher (P<0.05) in CWE. In conclusion, based on sperm motility and lipid per oxidation, TCAE was more efficient for cryopreservation of buffalo semen.

Pure and Applied Biology, Jun 10, 2022

Dystocia is one of the most common complications in parturition associated with high risk in Balo... more Dystocia is one of the most common complications in parturition associated with high risk in Balochistan. This condition causes considerable economic losses to local farmers, if not properly treated. These are mostly due to mixed breeding practices among different breeds, management problem as well as inaccessibility to veterinary practitioner. An adult local nondescript doe weighing 41 kg was presented to teaching veterinary hospital at Faculty of Veterinary and Animal Sciences (FVAS), Lasbela University of Agriculture Water and Marine Sciences (LUAWMS), Uthal. Animal had a history of difficulty in giving birth with straining since more than last 20 hours. On per-vaginal examination, the ruptured foetal membrane was present with fully dilated cervix. Similarly, bilateral hock flexion (Breech presentation) was diagnosed. No any foetal reflex was present during examination. Defective postured foetus was successfully taken out after adopted the traction procedure. Treatment was given to avoid any post-parturient infection. No any remarkable complications were seemed one week follow up of treatment. The purpose of this report is providing awareness among the livestock practitioners about incidence of dystocia cases, draw backs of management malpractices to be held in the mix breed herd in the Balochistan province.

Journal of Applied Animal Research

This study was designed to determine the effect of different osmotic pressures on spermatozoa cha... more This study was designed to determine the effect of different osmotic pressures on spermatozoa characteristics of cryopreserved buffalo bull semen using tris egg yolk extender (TEYE). Semen of Nili-Ravi buffalo bulls (n = 4) housed at a semen production unit was collected at weekly intervals for 10 weeks. Three solutions of tris-citric acid-fructose with osmotic pressures of 255, 275 and 295 mOsm/kg were used in extender preparation. Semen straws containing 20 × 10 6 spermatozoa were processed and stored at −196°C in liquid nitrogen. Post-thaw analyses of spermatozoa included motility, viability, acrosomal integrity, plasma membrane integrity, DNA integrity and lipid per-oxidation. Significantly higher (P < .05) sperm motility, acrosomal and DNA integrity were recorded at osmotic pressures of 275 and 295 mOsm/kg compared to 255 mOsm/kg. However, differences in spermatozoa viability, plasma membrane integrity and lipid per-oxidation were non-significant among three osmotic pressures. It is concluded that osmotic pressure of the solution used in extender preparation plays an important role in post-thaw quality of cryopreserved buffalo bull semen.

Artificial insemination with cryopreserved semen is the most viable biotechnology for faster and ... more Artificial insemination with cryopreserved semen is the most viable biotechnology for faster and increased genetic improvement in many species allowing for improved herd performance and productivity. Pakistan has 34.6 million buffalo which have major share in total milk produced in the country. Rapid increase in human population and demand for animal products motivated the researchers to increase per animal milk production using this biotechnology. In buffalo, natural breeding practice is common in the country compared to artificial insemination, low fertility rate with cryopreserved semen is main hindrance in its propagation. It is need of the hour to disseminate the knowledge of various factors and components contributing in buffalo semen cryopreservation to improve milk and fertility rate of this breed.

Buffalo Bulletin, Sep 26, 2022

In this study, efforts were made to investigate fresh semen parameters and to select a suitable e... more In this study, efforts were made to investigate fresh semen parameters and to select a suitable extender for buffalo semen cryopreservation. Experiment I, fresh undiluted seminal parameters were determined while Experiment II, efficacy comparison of coconut water extender (CWE) with tris citric acid extender (TCAE) and skimmed milk extender (SME) was made. Four bulls single ejaculate was collected weekly for 5 and 10 weeks for Experiment I and II, respectively however osmotic pressure replicates were 16 and 6 for semen and seminal plasma, respectively. In Experiment I, each bull spermatozoa concentration, motility (%), semen volume and pooled semen percentage NAR, PMI, viability and MTT (3-(4, 5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction rate was checked. In Experiment II, pooled semen added to extenders and then equilibrated (4 o C) and filled to obtain 20×10 6 spermatozoa/0.5 ml straws before plunging in liquid nitrogen. Percentage thawed spermatozoa viability, normal acrosomal ridge (NAR), motility, DNA damage, plasma membrane integrity (PMI) and lipid peroxidation (nM) recorded. In Experiment I, seminal parameters as spermatozoa concentration (1226.43±71.48 million/mL), semen volume (2.84±0.14 mL), viability (90.05±0.71%), motility (77.13±0.71%), PMI (86.23±0.34%), NAR (94.67±0.30%) and MTT reduction rate (0.290±0.06) while osmotic pressure of seminal plasma (294.83±3.87 mOsm/ kg) and semen (290.87±2.58 mOsm/kg) was recorded. In Experiment II, TCAE higher (P<0.05) sperm motility noted compared to CWE and SME whereas percentage viability, NAR, PMI, DNA damage was non-significant. Lipid peroxidation compared to SME and TCAE was higher (P<0.05) in CWE. In conclusion, based on sperm motility and lipid per oxidation, TCAE was more efficient for cryopreservation of buffalo semen.

Pure and Applied Biology, Jun 10, 2022

Dystocia is one of the most common complications in parturition associated with high risk in Balo... more Dystocia is one of the most common complications in parturition associated with high risk in Balochistan. This condition causes considerable economic losses to local farmers, if not properly treated. These are mostly due to mixed breeding practices among different breeds, management problem as well as inaccessibility to veterinary practitioner. An adult local nondescript doe weighing 41 kg was presented to teaching veterinary hospital at Faculty of Veterinary and Animal Sciences (FVAS), Lasbela University of Agriculture Water and Marine Sciences (LUAWMS), Uthal. Animal had a history of difficulty in giving birth with straining since more than last 20 hours. On per-vaginal examination, the ruptured foetal membrane was present with fully dilated cervix. Similarly, bilateral hock flexion (Breech presentation) was diagnosed. No any foetal reflex was present during examination. Defective postured foetus was successfully taken out after adopted the traction procedure. Treatment was given to avoid any post-parturient infection. No any remarkable complications were seemed one week follow up of treatment. The purpose of this report is providing awareness among the livestock practitioners about incidence of dystocia cases, draw backs of management malpractices to be held in the mix breed herd in the Balochistan province.

Journal of Applied Animal Research

This study was designed to determine the effect of different osmotic pressures on spermatozoa cha... more This study was designed to determine the effect of different osmotic pressures on spermatozoa characteristics of cryopreserved buffalo bull semen using tris egg yolk extender (TEYE). Semen of Nili-Ravi buffalo bulls (n = 4) housed at a semen production unit was collected at weekly intervals for 10 weeks. Three solutions of tris-citric acid-fructose with osmotic pressures of 255, 275 and 295 mOsm/kg were used in extender preparation. Semen straws containing 20 × 10 6 spermatozoa were processed and stored at −196°C in liquid nitrogen. Post-thaw analyses of spermatozoa included motility, viability, acrosomal integrity, plasma membrane integrity, DNA integrity and lipid per-oxidation. Significantly higher (P < .05) sperm motility, acrosomal and DNA integrity were recorded at osmotic pressures of 275 and 295 mOsm/kg compared to 255 mOsm/kg. However, differences in spermatozoa viability, plasma membrane integrity and lipid per-oxidation were non-significant among three osmotic pressures. It is concluded that osmotic pressure of the solution used in extender preparation plays an important role in post-thaw quality of cryopreserved buffalo bull semen.

Artificial insemination with cryopreserved semen is the most viable biotechnology for faster and ... more Artificial insemination with cryopreserved semen is the most viable biotechnology for faster and increased genetic improvement in many species allowing for improved herd performance and productivity. Pakistan has 34.6 million buffalo which have major share in total milk produced in the country. Rapid increase in human population and demand for animal products motivated the researchers to increase per animal milk production using this biotechnology. In buffalo, natural breeding practice is common in the country compared to artificial insemination, low fertility rate with cryopreserved semen is main hindrance in its propagation. It is need of the hour to disseminate the knowledge of various factors and components contributing in buffalo semen cryopreservation to improve milk and fertility rate of this breed.