Andre Felix - Academia.edu (original) (raw)

Papers by Andre Felix

Extraprensa, Jul 1, 2015

A construção da noção de campo da produção de bens simbólicos por Pierre Bourdieu, ao longo de su... more A construção da noção de campo da produção de bens simbólicos por Pierre Bourdieu, ao longo de sua trajetória, parte do princípio de que ele é um espaço de oposições e disputas internas protagonizadas por seus agentes. Ao mesmo tempo, esses integrantes do campo estabelecem relações de recusas e aderências ao modus operandi de outros campos, especialmente o campo econômico que apresenta-se, em muitos casos, como dominante na relação com os demais, todos inseridos no mesmo espaço social.

L'invention concerne un panneau 1 qui permet de fixer sans collage des plaques minces de reve... more L'invention concerne un panneau 1 qui permet de fixer sans collage des plaques minces de revetement de facades, en particulier des plaques de verre ou de pierre, avec un joint creux 5 entre les panneaux adjacents. Le panneau comporte un cadre 54' en metal leger qui entoure la plaque exterieure 50 du revetement. Ce cadre comprend au moins un profile 54 ayant un rebord continu 12 qui s'etend devant une zone peripherique de la surface exterieure 14 de la plaque. De preference, cette zone est en biseau 13. Le panneau est entierement fabrique en usine et il est equipe d'organes de fixation permettant un eventuel demontage ulterieur. Application aux revetements de facades des bâtiments, ainsi qu'aux fenetres.

Applied and Environmental Microbiology, 1982

Methylobacterium sp. strain CRL-26 grown in a fermentor contained methane monooxygenase activity ... more Methylobacterium sp. strain CRL-26 grown in a fermentor contained methane monooxygenase activity in soluble fractions. Soluble methane monooxygenase catalyzed the epoxidation/hydroxylation of a variety of hydrocarbons, including terminal alkenes, internal alkenes, substituted alkenes, branched-chain alkenes, alkanes (C 1 to C 8 ), substituted alkanes, branched-chain alkanes, carbon monoxide, ethers, and cyclic and aromatic compounds. The optimum pH and temperature for the epoxidation of propylene by soluble methane monooxygenase were found to be 7.0 and 40°C, respectively. Among various compounds tested, only NADH 2 or NADPH 2 could act as an electron donor. Formate and NAD + (in the presence of formate dehydrogenase contained in the soluble fraction) or 2-butanol in the presence of NAD + and secondary alcohol dehydrogenase generated the NADH 2 required for the methane monooxygenase. Epoxidation of propylene catalyzed by methane monooxygenase was not inhibited by a range of potentia...

Revista Extraprensa, 2015

European Journal of Biochemistry, 1979

Cell-free extracts derived from yeasts Candida utilis ATCC 26387, Hansenula polymorpha ATCC 26012... more Cell-free extracts derived from yeasts Candida utilis ATCC 26387, Hansenula polymorpha ATCC 26012, Pichia sp. NRRL-Y-11328 Torulopsis sp. strain A1 and Kloeckeva sp. strain A2 catalyzed an NAD '-dependent oxidation of secondary alcohols (2-propano1, 2-butanol, 2-pentano1, 2-hexanol) to the corresponding methyl ketones (acetone, 2-butanone, 2-pentanone, 2-hexanone). We have purified a NAD '-specific secondary alcohol dehydrogenase from methanol-grown yeast, Pichia sp. The purified enzyme is homogenous as judged by polyacrylamide gel electrophoresis. The purified enzyme catalyzed the oxidation of secondary alcohols to the corresponding methyl ketones in the presence of NAD' as an electron acceptor. Primary alcohols were not oxidized by the purified enzyme. The optimum pH for oxidation of secondary alcohols by the purified enzyme is 8.0. The molecular weight of the purified enzyme as determined by gel filtration is 98000 and subunit size as determined by sodium dodecyl sulfate gel electrophoresis is 48000. The activity of the purified secondary alcohol dehydrogenase was inhibited by sulfhydryl inhibitors and metal-binding agents.

The Journal of General and Applied Microbiology, 1979

Soluble cytochrome c from Methylomonas methanica was purified to homogeneity and its properties w... more Soluble cytochrome c from Methylomonas methanica was purified to homogeneity and its properties were characterized. The molecular weight as determined by gel filtration is 18,000 and the subunit size as determined by sodium dodecyl sulfate gel electrophoresis is 17,000. The amino acid composition and spectral properties of cytochrome c are presented. Antisera prepared against the purified cytochrome from Methylomonas methanica cross-reacted with cytochrome c in cell extracts of various obligate methane-utilizing bacteria.

Canadian Journal of Microbiology, 1981

Cell suspensions of methane-utilizing bacteria oxidized n-alkanes (propane, butane, pentane, and ... more Cell suspensions of methane-utilizing bacteria oxidized n-alkanes (propane, butane, pentane, and hexane) to their corresponding alcohols and methyl ketones. The product alcohols and methyl ketones accumulated extracellularly. Methanol-grown cells of methane-utilizing bacteria did not oxidize n-alkanes. The product primary alcohol was detected in a cell-free system but only in a trace amount in the whole cell system due to further oxidation. The optimum conditions for in vivo formation of secondary alcohol and methyl ketone from n-alkanes were compared between two distinct types of C1-utilizing microbes: Methylococcus capsulatus M1 (type I membrane) and Methylosinus trichosporium OB3b (type II membrane). The production of acetone or 2-butanone from n-alkanes ceased after 3 h of incubation for strain OB3b and 5 h for strain M1. The amount of these methyl ketones did not decline during 30 h of incubation. The optimum pH for the in vivo production of methyl ketones from n-alkanes by bot...

Bioorganic Chemistry, 1978

Abstract Circular dichroism (CD) spectra of catechol 1,2-dioxygenase from Acinetobacter calcoacet... more Abstract Circular dichroism (CD) spectra of catechol 1,2-dioxygenase from Acinetobacter calcoaceticus exhibit three positive ellipticity bands between 240 and 300 nm (250, 283, and 292 nm), two negative bands at 327 and 480 nm, and a low-intensity positive band at 390 nm. The fractions of helix β-form, and unordered form of the enzyme are 8, 38, and 54%, respectively. The circular dichroic bands at 327 and 480 nm and a part of the positive bands at 292 and 390 nm are associated with enzyme activity. Significant changes in absorption and CD spectra of the enzyme were observed when the temperature of the enzyme preparation was increased to 47°C, coinciding with the sharp decrease in enzyme activity observed at this temperature.

Archives of Microbiology, 1979

Procedures for the purification of an aldehyde dehydrogenase from extracts of the obligate methyl... more Procedures for the purification of an aldehyde dehydrogenase from extracts of the obligate methylotroph, Methylomonas methylovora are described. The purified enzyme is homogeneous as judged from polyacrylamide gel electrophoresis. In the presence of an artificial electron acceptor (phenazine methosulfate), the purified enzyme catalyzes the oxidation of straight chain aldehydes (C1--C10 tested), aromatic aldehydes (benzaldehyde, salicylaldehyde), glyoxylate, and glyceraldehyde. Biological electron acceptors such as NAD+, NADP+, FAD, FMN, pyridoxal phosphate, and cytochrome c cannot act as electron carriers. The activity of the enzyme is inhibited by sulfhydryl agents [p-chloromercuribenzoate, N-ethylmaleimide and 5,5-dithiobis (2-nitrobenzoic acid)], cuprous chloride, and ferrour nitrate. The molecular weight of the enzyme as estimated by gel filtration is approximately 45000 and the subunit size determined by sodium dodecyl sulfate-gel electrophoresis is approximately 23000. The purified enzyme is light brown and has an absorption peak at 410 nm. Reduction of enzyme with sodium dithionite or aldehyde substrate resulted in the appearance of peaks at 523 nm and 552nm. These results suggest that the enzyme is a hemoprotein. There was no evidence that flavins were present as prosthetic group. The amino acid composition of the enzyme is also presented.

… para América Latina, 2006

... André Félix; André Santos; Anna Cristina; Carina Elizabeth Velozo Schmiedecke; Débora Pláton;... more ... André Félix; André Santos; Anna Cristina; Carina Elizabeth Velozo Schmiedecke; Débora Pláton; Fernanda Cunha; Liege; Mariah ... da subjetividade contemporânea; tendo como dispositivos de análises, filósofos e teóricos modernos (Focault, Deleuze, Bauman)ea Psicanálise ...

Journal of Bacteriology, 1976

Obligate methylotrophs are divisible into two types on the basis of ultrastructural biochemical c... more Obligate methylotrophs are divisible into two types on the basis of ultrastructural biochemical characteristics. Both groups possess a soluble phenazine methosulfate (PMS)-dependent methanol dehydrogenase. In addition, particulate PMS-dependent methanol dehydrogenase and PMS-independent methanol oxidase have been found in the type I membrane group. A procedure was developed for the crystallization of methanol dehydrogenase from the soluble fraction of the type II obligate methylotroph Methylosinus sporium. This is the first report of a crystalline methanol dehydrogenase from a methylotrophic bacterium. The crystallized enzyme is homogeneous as judged by ultracentrifugation and by acrylamide gel electrophoresis. In the presence of an electron acceptor (phenazine or phenazinium compound) and an activator (ammonium compound), the crystallized enzyme catalyzed the oxidation of primary alcohols and formaldehyde. Secondary, tertiary, and aromatic alcohols were not oxidized. The molecular ...